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ZEBRAFISHVolume 4, Number 1, 2007© Mary Ann Liebert, Inc.DOI: 10.1089/zeb.2006.9997
Zebrafish in the Wild: A Review of Natural History andNew Notes from the Field
RAYMOND E. ENGESZER, LARISSA B. PATTERSON, ANDREW A. RAO, and DAVID M. PARICHY
ABSTRACT
The zebrafish, Danio rerio, has emerged as a major model organism for biomedical research, yet little is knownabout its natural history. We review the literature pertaining to the geographic range, biotic and abiotic habitats,and life cycle of the zebrafish. We also report our own field study to document several aspects of zebrafish nat-ural history across sites in northeast India. We found zebrafish particularly abundant in silt-bottomed, well-veg-etated pools and rice paddies adjacent to slow moving streams at a range of elevations. We further identified co-occurring fishes likely to be zebrafish competitors and predators. Finally, we present observations that indicatesubstantial habitat degradation and loss, and suggest guidelines for documenting and preserving natural zebrafishpopulations.
21
INTRODUCTION
AKNOWLEDGE OF ZEBRAFISH NATURAL HISTORY
and ecology is critical for interpreting itsbehavior and physiology, extant genetic andphenotypic variation, and the evolutionary his-tory of embryonic, larval, and adult traits. Yetwe know surprisingly little about the naturalenvironment of the zebrafish or how it interactswith that environment,1 despite humans andzebrafish having shared waterways around theriver Ganges for tens of thousands of years.2
Like most other biomedical model organ-isms, the zebrafish was chosen for particulartraits that make it convenient for laboratorystudy, not for a broad understanding of the or-ganism in its native environment.3–6 Despitethe wealth of information on developmentaland genetic mechanisms and the arsenal of re-sources and techniques for studying zebrafish,this species remains underexploited for organ-ismal research, comprising studies of ecology,
evolution, and behavior. More fully realizingthe potential of zebrafish for organismal biol-ogy, as well as integrative studies spanningmultiple levels of organization, requires someknowledge of zebrafish natural history: its ge-ographical distribution, physical habitat, diet,and its competitors and predators.
We review the scant literature on zebrafishin the wild and document our own recent workaimed at developing a deeper understandingof zebrafish natural history. We present a hy-pothesis for zebrafish life history in the wild,suggest how studies of natural populations caninform research in the laboratory, and makerecommendations for future work in this area.
LITERATURE REVIEW
An extensive literature exists on Central Asianfishes and specifically on Indian fishes.7–13 Yet,these works focus on taxonomy and regional
Department of Biology, University of Washington, Seattle, Washington.
species lists, and contain little or no informa-tion on natural history, development and lifehistory, ecology, or behavior, except for someeconomically important fishes and sport fishes.For zebrafish, there is only meager publishedinformation, aside from studies of taxonomyand phylogenetic relationships.14–21
Even the geographic range of zebrafish is amatter of conjecture.22,23 From original collec-tions data, we can reconstruct a range extend-ing from Pakistan in the west to Myanmar(Burma) in the east, and from Nepal in the northto the Indian state of Karnataka in the south(Fig. 1) (Appendix). Nevertheless, records forthe extremes of this distribution are mostly out-dated. For example, the last recorded collectionof zebrafish in Myanmar occurred in 1926, de-spite successful collections of similar fishes inthat country more recently. Given the rapidlyexpanding human population in Central Asiasince the early twentieth century and the con-comitant negative anthropogenic effects onfreshwater ecosystems, historical records maynot reflect the current zebrafish range. More-over, some specimens are likely to have beenmisidentified, particularly at the extremes of therange (T. Roberts, personal communication).
What is the typical habitat of wild zebrafish?Several sources agree that zebrafish are foundin rivers, small streams and other channels,stagnant or slow-moving pools near streams,and rice paddies.1,12,23,24 More precise infor-mation is mostly lacking. For example, severalworks on regional Indian fauna include waterquality data for river systems that contain ze-brafish, yet these measurements cannot be re-lated to the particular microhabitats where ze-brafish or other species reside (e.g., fast flowingmain river channels vs. slow flowing or stag-nant streamside pools).24-26 However, a re-cently published study presents data collectedin September and October 1995 from three siteswhere zebrafish were found in the northernand northeastern India states of Uttar Pradeshand West Bengal.27 Although data from indi-vidual sites were not presented, pooled dataacross all three sites associate zebrafish withrelatively still water (currents, 0 m–sec to 0.1m–sec) at 27°C to 34°C and pH 7.9–8.2; widthsof water bodies ranged from 1 to 12 m, anddepths ranged from 16 to 57 cm; water was rel-
atively clear (transparent to �35 cm), over sub-strates of clay, silt, cobble, or boulders. Thus,previous works suggest some general featuresof zebrafish habitats in the wild, though it isnot possible to associate data for multiple pa-rameters with specific zebrafish localities.
Biotic features of the zebrafish habitat aresimilarly undocumented. Extensive collectionsdata exist, yet they are not sufficiently detailedto indicate the extent to which zebrafish co-oc-curred with particular species at particularsites. Thus, it is virtually impossible to inferwhich species might be predators or competi-tors of zebrafish in its native environment. Ze-brafish themselves are known to feed on mos-quito larvae28 and, presumably, other insects,though the precise species are not known.27 Al-though vegetation can provide cover from ter-restrial and aquatic predators, and microhabi-tats for spawning and foraging, the types andextent of vegetation in the zebrafish environ-ment is not known, although canopy coverranged from 0% to 50% across the three sitesmentioned above.27
A final and critical aspect of zebrafish nat-ural history is its behavior and life cycle. Thebreeding season is reportedly between Apriland August,1 presumably varying somewhatby latitude, elevation, and prevailing climaticconditions. Egg laying is thought to occur in small pools adjacent to streams.23 Spawningbehavior itself has been described only in thelaboratory.29,30 Anecdotal comments suggestthat individuals hatch within 3 days postfertil-ization, but may take as long as 5–6 months toreach reproductive maturity in the wild.1,23
This brief review indicates that much of ze-brafish natural history awaits discovery, andthere are exciting opportunities to learn basicfeatures of this species’ behavior, ecology, andevolution. Such information is valuable in itsown right, and also sets the stage for integra-tive research programs made feasible by themany resources accompanying this biomedicalmodel organism.
OBSERVATIONS FROM THE FIELD
To document the abiotic and biotic habitat ofzebrafish and to better understand its natural
ENGESZER ET AL.22
history, we sought wild populations across arange of geographical and elevational localitiesin the northeast Indian states of West Bengal,Assam, Meghalaya, and Orissa (Table 1). At alllocalities, we recorded global positioning sys-tem (GPS) coordinates and elevation; we tookphotographs and sketched geographical fea-tures; we measured water temperature, pH,and conductivity; and we made qualitative ob-servations of water clarity, substrate type, rateof flow, and extent of vegetation. To assess thefish communities in which zebrafish occurs, wecollected and imaged fishes at each locality,and we identified them to genus and specieswhen possible, or to genus alone for species notyet formally described.
Throughout the trip we worked with localfisherman, who typically used box seines. Atsome sites these were supplemented withlarger seines, gill nets, or minnow traps. Dif-ferent fishing methods bias collections towardsparticular types of fishes, and our seines andtraps frequently were most suitable for smallerspecies, like zebrafish. Thus, our collections arenot exhaustive, although we did often recover
juveniles of much larger species that would nototherwise have been trapped using our meth-ods. We employed local fisherman partly fortheir regional expertise, but primarily for theirfamiliarity with the inhabitants of the localfarms and villages. Many sites are used for sub-sistence fishing of Barilius barilius, Notopterusnotopterus, Tor progenius, and other species, andfor this reason access is guarded jealously, re-gardless of the intended catch. Sites were onlyfished after we received the blessings of localleaders. Fish were returned alive to their sitesof capture immediately after imaging and iden-tification.
Itinerary
We began our expedition in Kolkata (for-merly Calcutta), the capital of West Bengal, inearly July 2006 during the monsoon season.Our goal was visit as wide a range of sites ascould be achieved in 17 days.
West Bengal and Assam. After one night inKolkata, we traveled by air to Bagdogra in thenortheast corner of West Bengal (Fig. 2A, B and
ZEBRAFISH IN THE WILD 23
FIG. 1. Historical collections of zebrafish in India and neighboring countries since 1868.
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/06
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55
a Onl
y si
tes
wit
h ze
braf
ish
or o
ther
fis
hes
are
liste
d.
b Ele
vati
on in
met
ers.
c Con
duc
tivi
ty.
dC
ond
itio
ns in
Jan
uary
200
6, m
id-m
orni
ng: 2
0.5°
C, p
H 7
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P. C
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e, p
erso
nal e
-mai
l com
mun
icat
ion
on 8
/16
/06
).e C
ond
itio
ns in
Jan
uary
200
6, m
id-m
orni
ng: 1
8.9°
C, p
H 7
.9 (
P. C
ottl
e, p
erso
nal e
-mai
l com
mun
icat
ion
on 8
/16
/06
).
Table 1). Localities here were relatively low-ly-ing, with elevations ranging from 39 m to 63 mabove sea level. Our first day of field work be-gan in the Buxa Tiger Reserve, about 10 kmnortheast of Barovisha, a “village” with over500,000 people, yet too small to appear on ourmaps. After hiking past stinging nettles largerthan dinner plates, we reached the Jorai river(sites 1 and 2). During the monsoon, seasonalstreams known as nalas feed into larger riverssuch as the Jorai, and it was in one of these trib-utaries that we netted our first zebrafish. At thissite we also collected terrestrial leeches, thoughunintentionally.
The same day we stopped at the Suthimaririver (sites 3–6, Fig. 2B), a larger tributary ofthe Jorai. While recording water quality in theSuthimari (Fig. 3A), we observed runoff fromnearby rice paddies that markedly raised thetemperature in that area of the river (Fig. 3B).Further examination of these paddies revealedvast numbers of zebrafish juveniles and adults.Despite a water temperature of 38.6°C, six de-grees higher than the main river a few metersaway, zebrafish were active, seemingly healthy,and showed no obvious signs of stress such aslabored gilling or gasping at the surface. Hereand elsewhere, we found zebrafish only in rice paddies with crops that were mature or al-ready harvested; fish were not found in freshlyplanted paddies. The Suthimari also had sur-prises such as freshwater pufferfish (Tetraodoncutcutia) and gravid male pipefish (Microphisdeocata) (see below). That afternoon we experi-enced our first, and only, monsoon shower inthe field.
Additional sites in West Bengal yielded awide variety of species, including more ze-brafish. A trip to Lefraguri swamp (site 11, Fig.3E), in the Buxa Tiger Reserve, 8 km north ofBarovisha, rewarded us with zebrafish, a pre-viously seen but undescribed loach (Lepido-cephalus), and an entirely new species of pis-civorous snakehead (Channa), unusual in itscomplete lack of pelvic fins. We also collectedtwo larger species related to zebrafish, D. dan-gila and Devario devario (see below). Adults ofboth species are considerably larger than ze-brafish and seem likely to compete with oneanother for food or habitat. Larvae and juve-niles of D. dangila also are nearly indistin-
guishable from zebrafish (unpublished data),and might compete with equivalently stagedzebrafish; we never found D. dangila at siteswith abundant zebrafish.
As the sun set on our final day in West Ben-gal, we visited the Ghotimari river (site 12, Fig.3C, D), a slow moving, silt-bottomed tributaryof Rydak II, 15 km northwest of Barovisha. TheGhotimari and adjacent pools contained ze-brafish at higher abundance than any other sitewe visited in West Bengal, and our catch in-cluded both juveniles and adults. This sitelacked obvious piscivorous fishes or otherDanio or Devario species.
A single locality in the adjacent state of As-sam (site 13, Fig. 2C), 90 km east of Barovisha,was known by local fisherman to harbor ze-brafish, though we found none on the occasionwe visited. Political unrest in Assam made fur-ther exploration unwise and we continued on.
Meghalaya. To assess the elevational distri-bution of zebrafish, we continued by car toGuwahati, the capital of Assam, and then to thehilltop city of Shillong, capital of the state ofMeghalaya. Our localities here ranged from1234 m to 1795 m above sea level. After stiflingheat in the plains, the cooler, mist-shroudedhills of Meghalaya were a welcome respite.Seinpoh stream (sites 14–16, Fig. 2D) yieldedtremendous numbers of zebrafish, includingjuveniles and adults (Fig. 4A). Though close toa road and to farms, the site appeared other-wise relatively free of human disturbance.Seinpoh stream itself had a gentle flow and arock bottom; we found only small numbers ofzebrafish in the stream proper. By contrast, aswampy pool connected to the stream had lit-tle or no flow, a silt bottom, and abundant veg-etation (Fig. 4C); we observed vast numbers ofjuvenile and adult zebrafish shoaling in thispool (Fig. 4D). We also found large numbers ofzebrafish in nearby rice paddies. Notably, ze-brafish in the water were easily distinguishablefrom other species by their cranial patch ofreflective iridophores (Fig. 4D). Although wedid not find Channa or other piscivorous fishes,we did collect the large zebrafish relative, D.meghalayensis.
Despite the abundance of zebrafish at Sein-poh, most of the high elevation streams and
ENGESZER ET AL.26
rivers in Meghalaya had faster flows, with rockor gravel bottoms, and relatively little vegeta-tion. We found zebrafish at only one other lo-cality, the Dukan river (site 18, Fig. 4B), whichhad a gentler flow and some overhangingvegetation, similar to Seinpoh. After severaldays in Meghalaya, we drove from Shillongback to Guwahati, and then returned by air toKolkata.
Orissa. To expand our latitudinal range, wetraveled by car southwest from Kolkata toTarania village (sites 23–26, Fig. 2E) in the stateof Orissa near the border with West Bengal.Like the areas we visited in West Bengal, Tara-nia is at low elevation with extensive agricul-ture and numerous rice paddies. Sites in andaround the village included ponds and flooded
rice paddies. These ponds were silt bottomedwith some submerged vegetation, similar tosites where we had previously found zebrafish.In contrast to other sites, however, visibility inthe ponds was very low (�3 cm) owing to al-gae as well as recent heavy rains (Fig. 5). Whilezebrafish were caught in 2 of the 4 bodies ofwater we fished, they were less abundant thanat Seinpoh in Meghalaya or Ghotimari in WestBengal. In addition to finding zebrafish, wealso caught the largest fish of the trip, an adultpiscivore, Notopterus notopterus, �35 cm stan-dard length.
Abiotic environment summary
The 14 of 28 sites where we found zebrafishhad slow or still waters at 24.6°C to 38.6°C, pH
ZEBRAFISH IN THE WILD 27
FIG. 2. Localities visited during July 2006 survey of zebrafish habitat and natural history. (A) Overview of regionin northeast India, bordered by Bhutan, Bangladesh, and Myanmar. Scale bar, 100 km. Details of boxed regions areshown in figures B–E. Legend: Orange circles, sites where zebrafish were found. Blue circles, sites without zebrafish.Site numbers reflect the approximate sequence in which sites were visited; locality details and GPS coordinates arelisted in Table 1. (B) Northern West Bengal low elevation localities with zebrafish found at 8 of 12 sites. The locationof Barovisha is approximate. Scale bar, 5 km. (C) Assam low elevation locality in which no zebrafish were found,though local fisherman had found them there within the previous year. Scale bar, 2 km. (D) Meghalaya high eleva-tion localities. Zebrafish were found at only 2 of 7 sites visited, probably owing to habitat loss. Scale bar, 10 km. (E)Orissa and southern West Bengal low elevation localities. Zebrafish were found in 2 of 4 closely situated sites in Orissabut were not found at sites just outside of Kolkata in West Bengal, where they have been found within the past 30years. Scale bar, 20 km.
5.9–8.1, and conductivities of 10 �S to 271 �S.All but two of these sites had silt-covered bot-toms with submerged or overhanging vegeta-tion. On the two occasions we located zebrafishin rocky bottomed streams (sites 14 and 18),they were found in small numbers, shoalingonly near the bank under overhanging vegeta-tion. We found zebrafish in relatively clear wa-ters, except for the turbid pools in Orissa.
Biotic environment summary
Zebrafish co-occurred with a wide variety offishes, from brightly colored barbs to the drolland bewhiskered loaches. The fish species col-lected at sites with zebrafish are shown in Fig.6A. Selected fishes collected at sites where ze-brafish was absent but other Danio or Devariowere present are shown in Fig. 6B and fishesfrom all sites are listed in Table 2. While oursampling was not intended to be exhaustive,our observations nevertheless suggest candi-date competitors and predators.
The fishes most likely to compete with ze-brafish are other minnows of the same familyas zebrafish, Cyprinidae. A wide variety ofcyprinids co-occur with zebrafish, both smallfishes like the barbs, Puntius, and larger fisheslike the hill trouts, Barilius. We regularly foundother Danio or Devario species and, in somecases, both, syntopic with zebrafish. Esomusdanricus may be a principal competitor with ze-brafish for food, as both species are similarlysized, with similar gapes, and occupy similar,high positions in the water column; when wefound E. danricus, we found them in large num-bers. Aplocheilus panchax, though slightly largerthan zebrafish, also swims high in the watercolumn and could compete with zebrafish forfood. Morever, E. danricus, A. panchax, and ze-brafish all feed on insects.27,28 If there is com-petition for breeding sites and larval habitats,then Puntius shalynius would be an obviouscandidate for such a role, as we observed thesefish spawning in rice paddies that were swarm-ing with juvenile zebrafish.
A number of species we collected are likelyto prey on zebrafish, though analyses of gutcontents will be needed to test these inferences.The snakeheads, Channa, the needlefish, Xe-nentodon cancila, the catfish, Mystus bleekeri, and
the knifefish, N. notopterus, all co-occur with ze-brafish and have gapes sufficient to swallowadults. Previous gut content analyses showedthat a large proportion of the diet of these fishesconsists of small fishes31; at sites with these pis-civorous fishes, zebrafish were either absent oroccurred at very low abundance. We speculatethat tire-track eel, Mastacembelus armatus, mayfeed incidentally on zebrafish embryos orhatchlings: other mastacembelids are oopha-gous32 and presumably find eggs while prob-ing the substrate for benthic invertebrates.While the long-tailed catfish, Olyra longicau-data, certainly has a gape large enough to takeadult zebrafish, this species tends to occupy rif-fles and faster moving waters where zebrafishare not found. Another potential predator, theswamp eel, Monopterus cuchia, has a tiny mouthfor its size and would be less likely to feed suc-cessfully on zebrafish adults.
We did not find large predatory fishes in ricepaddies or shallow seasonal waters where juve-nile zebrafish were abundant. Nevertheless,even these apparent refuges harbored aquaticdragonfly larvae. Odonate larvae are voracious,visually oriented predators of larval fish in fresh-water environments and could play a significantrole in juvenile zebrafish mortality.33–36 Adultdragonflies were plentiful at every site we vis-ited and we often collected their aquatic larvaewith zebrafish. Other aquatic invertebrates maybe predatory on larval zebrafish as well.37,38
Habitat degradation and loss
An unanticipated finding of our field surveywas the degree to which anthropogenic factorshave affected zebrafish habitat, both in the dis-tant past and quite recently. An historical in-fluence has surely been rice cultivation. Aswaterways were dammed to allow irrigation,many seasonal streams and wetlands wouldhave been lost. Fortunately for zebrafish, thespecies appears to reproduce just as well in ricepaddies as in seasonal nalas. Nevertheless,these changes probably have altered the bioticcommunity, as some competitors or predatorswould have been more adept than others at col-onizing these habitats. A second agriculturalpractice that affects zebrafish is the productionof jute, Corchours capsularis, a crop that is the
ENGESZER ET AL.28
FIG. 3. Zebrafish localities in northern West Bengal. (A) At the Suthimari river (sites 3–5), a wide variety of fish specieswere collected from the main portion of the river. (B) Adjacent rice paddies that drain into the main waterway of theSuthimari river (site 6). Paddies are in different states of cultivation. Numerous juvenile zebrafish were present in thepaddy at the upper left. This shallow water reached 38.6°C in the afternoon sun. (C, D) The Ghotimari river (site 12)had zebrafish in the greatest abundance of all West Bengal sites. (C) The main stream had a slow current and extensivevegetation on the banks. Left to right, L. Patterson, local fisherman, R. Engeszer, A. Rao. (D) Shallow pools adjacent tothe stream harbored juvenile and adult zebrafish in similar or greater abundance. Fisherman “drive” fish towards a tri-angular box seine. (E) Lefraguri swamp (site 11), a swampy habitat with no discernable water flow, yielded small num-bers of zebrafish. Images in Figures 3–7 were recorded with a Nikon D200 digital single lens reflex camera equippedwith a Nikon 105 mm f/2.8G ED-IF AF-S VR Micro-Nikkor or a Nikon 18–200 f/3.5–5.6G DX VR Zoom-Nikkor, mountedon a Gitzo tripod. Additional images can be seen at http://protist.biology.washington.edu/dparichy.
FIG. 4. Zebrafish localities in Megha-laya. (A) Seinpoh stream (site 14), asmall, slow moving stream in relativelyundisturbed habitat of meadow andtrees, is a high elevation site in north-ern Meghalaya. (B) Dukan river (site18) is a high elevation site in the south-ern hills of Meghalaya, immediately tothe north of much lower-lyingBangladesh. Only a few adult zebrafishwere collected in this small but swiftlymoving stream. (C) A pool (site 16) ad-jacent to the Seinpoh stream in A, withabundant vegetation, virtually stagnantwater, and numerous juvenile andadult zebrafish. (D) Close-up of shoal-ing zebrafish in C. A movie of thesesame fish can be found at http://protist.biology.washington.edu/dparichy.
ZEBRAFISH IN THE WILD 29
source of fiber used in clothing, sacks, twine,and other items. Once harvested, jute is curedin small streams, where it acidifies the water,causing fish mortality. More recently, the agri-cultural lands and native wetlands are them-selves being converted rapidly for housing andindustrial use around cities such as Kolkataand other population centers.
We observed several other dramatic examplesof habitat degradation and loss in Meghalaya.At one site, for example, strip mining for limeresulted in extensive pollution in a neighboringstream (Fig. 7A). Although we had collected ze-brafish in an adjoining clear stream (Fig. 4B), wefound no animal or plant life downstream of theconfluence of the two streams. Coal mining andcoal storage appear to have similar detrimentaleffects, as we found streams black with coal dust
and completely free of fish (Fig. 7B). Yet, evenseemingly more innocuous practices appear todegrade zebrafish habitat. One example is theintroduction into streams of detergents used forwashing laundry, a common practice that deci-mates the local fish fauna (Fig. 7C). Another dis-turbing practice is the relatively recent and un-sustainable method of fishing for food speciesby poisoning entire stretches of streams with in-dustrial reagents.
Having come to view the zebrafish as a hardyspecies in the laboratory, we had assumed itwould be equally resilient in the field, andmany of our observations were consistent withthis assumption: we found zebrafish at hightemperatures and across a broad pH range. Yeteven a fish as robust as zebrafish faces seriouschallenges from habitat degradation and loss.
ENGESZER ET AL.30
FIG. 5. Zebrafish localities in Orissa. (A) Tarania village (sites 24 and 25): ponds kept flooded by local villagers serveas habitat for food fish such as N. notopterus. Zebrafish were found in the pond to the left of the mud walkway. Notethe proximity to human habitation. (B) Local fisherman using a seine net to catch zebrafish at site 24. One fishermanswims to the far end, and will drive fish back towards the net, moving in the opposite direction. The pond was cov-ered with algae and had essentially no visibility, yet it yielded numerous zebrafish. (C) Fishermen examining thecatch at site 25.
FIG. 6. Fish species identified across all sites. Species are grouped by family (listed above images). (A) Species co-occuring in the same water bodies as zebrafish. (B) Selected species not found with zebrafish but co-occurring withother species of Danio or Devario. Overall, species in the family Cyprinidae were caught most often, although nu-merous species of loaches (Cobitidae) and snakeheads (Channidae) were found as well. A detail is shown of teeth inthe belonid Xenentodon cancila, a presumptive predator of zebrafish. The individual shown is a juvenile. Another de-tail is shown of the syngnathid pipefish Microphis deocata, revealing developing embryos in the pouch of a pregnantmale. Devario acuticephela was obtained in Kolkata and was probably collected in the state of Minapur. Scale bars inall images, 10 mm.
ZEBRAFISH IN THE WILD 31
TA
BL
E2.
SPE
CIE
SO
CC
UR
RE
NC
EB
YSI
TE
Site
s w
ith
zebr
afis
hSi
tes
wit
hout
zeb
rafis
h
Fam
ily/s
peci
es1
34
56
911
1214
1516
1824
262
78
1013
1719
2021
2223
2527
28
Cyp
rini
dae
Dan
io r
erio
XX
XX
XX
XX
XX
XX
XX
Am
blyp
hary
ngod
on m
ola
X
Bar
ilius
bar
ilaX
Bar
ilius
bar
naX
XX
Bar
ilius
ben
delis
isX
Che
la l
aubu
caX
Dan
io d
angi
laX
XX
Dan
io m
egha
laye
nsis
XX
X
Dan
ione
lla s
p.X
Dev
ario
dev
ario
XX
X
Dev
ario
ass
amen
sis
XX
Dev
ario
aeq
uipi
nnat
usX
X
Eso
mus
dan
ricu
sX
XX
Gar
ra l
isso
rhyn
chus
X
Labe
o ba
taX
Labe
o ro
hita
X
Ore
icht
hys
cosu
atis
X
Psi
lorh
ynch
us s
ucat
ioX
Pun
tius
cho
laX
Pun
tius
con
chon
ius
X
Pun
tius
phu
tino
XX
Pun
tius
sha
lyni
usX
X
Pun
tius
sop
hore
XX
Pun
tius
sp.
XX
Pun
tius
tic
toX
X
Tor
pro
geni
us
X
Ad
rian
icht
hyid
ae
Ory
zias
sp.
XX
X
Ana
bant
idae
Ana
bas
test
udin
eus
XX
X
Apl
oche
ilid
ae
Apl
oche
ilus
panc
hax
XX
XX
Bad
idae
Bad
is b
adis
X
Bad
is b
losy
rus
X
Bad
is s
p.X
Dar
io d
ario
XX
X
Dar
io s
p.X
Bag
rid
ae
Mys
tus
blee
keri
X
Mys
tus
teng
ara
X
Mys
tus
vitt
atus
X
Bal
ator
idae
Aca
ntho
cobi
tis
boti
aX
Lepi
doce
phal
us a
nnan
dale
iX
X
Lepi
doce
phal
us g
unte
aX
X
Lepi
doce
phal
us s
p. 1
X
XX
X
Lepi
doce
phal
us s
p. 2
X
X
Schi
stur
a ba
rapa
nien
sis
Schi
stur
a sa
vona
X
Schi
stur
a sp
.X
Bel
onid
ae
Xen
ento
don
canc
ilaX
X
Cha
nnid
aeX
Cha
nna
gach
uaX
XX
Cha
nna
punc
tata
X
Cha
nna
sp. 1
X
Cha
nna
sp. 2
X
Cha
nna
stew
arti
iX
Cha
nna
stri
ata
X
(con
tinu
ed)
Gob
iidae
Glo
ssog
obiu
s gi
uris
X
Mas
tace
mbe
lidae
Mac
rogn
athu
s ar
alX
Mac
rogn
athu
s pa
ncal
usX
X
Mac
rogn
athu
s sp
. X
Mas
tace
mbe
lus
arm
atus
X
Not
opte
rid
ae
Not
opte
rus
noto
pter
usX
Oly
rid
ae
Oly
ra l
ongi
caud
ata
X
Osp
hron
emid
ae
Col
isa
fasc
iata
XX
Col
isa
lalia
XX
XX
Col
isa
sota
X
Cte
nops
nob
ilis
X
Para
mba
ssid
ae
Par
amba
ssis
lal
aX
Par
amba
ssis
ran
gaX
XX
Siso
rid
ae
Har
a je
rdon
iX
Syng
nath
idae
Mic
roph
is d
eoca
taX
X
Synb
ranc
hid
ae
Mon
opte
rus
cuch
iaX
Tet
raod
onit
idae
Tet
raod
on c
utcu
tia
X
TA
BL
E2.
SPE
CIE
SO
CC
UR
RE
NC
EB
YSI
TE
(CO
NT.)
Site
s w
ith
zebr
afis
hSi
tes
wit
hout
zeb
rafis
h
Fam
ily/s
peci
es1
34
56
911
1214
1516
1824
262
78
1013
1719
2021
2223
2527
28
ZEBRAFISH IN THE WILD 35
DISCUSSION
Our limited field observations allow us tohypothesize the following life history for ze-brafish. During the year, adults occupy shallow
vegetated areas and areas shaded by over-hanging vegetation in streams proper. While inthe streams, they feed primarily on insects andare themselves susceptible to a variety of fishessuch as Channa. With the onset of the rainy sea-son, adults move into nalas and nearby floodedareas, including rice paddies. Spawning thenoccurs amid flooded vegetation in relativelystill, shallow waters, with silt-covered bottoms.Larvae and juveniles remain in these seasonalwaters as they develop, likely gaining somerefuge from piscivorous fishes, though experi-encing predation by invertebrates and particu-larly odonate larvae. Subsequently, young ze-brafish move back into the streams proper asthe seasonal waters recede. This model of ze-brafish natural history and the zebrafish life cy-cle should be testable with additional study inthe field.
Naturally occurring populations offer awealth of information and the tools to addressa variety of questions in both organismal andbiomedical research. For example, geneticallydistinct populations can provide insights intogenes responsible for natural phenotypic vari-ation39–41 and the effects of domestication.42,43
Such populations also are a valuable sourcefrom which to create inbred lines for geneticmapping. Moreover, natural populations har-bor mutant phenotypes (unpublished data)that supplement genetic screens for inducedmutations.44 Indeed, even mutant phenotypesisolated in the laboratory can vary in pene-trance and expressivity across genetic back-grounds,45–49 and such variability allows mod-ifier loci to be identified. Wild populations arean important reservoir for such genetic varia-tion. More generally, any attempt to under-stand the function or evolution of a phenotypictrait must address the environment in whichthe organism exists.50–52
As developmental genetic studies of ze-brafish increasingly address larval and adultphenotypes, an understanding of natural his-tory will provide a critical perspective on theforms taken by such traits within zebrafish, aswell as variations in form both within zebrafishand between species.6 For example, teleostadult pigment patterns influence shoaling,schooling, mate recognition, mate choice, andpredator avoidance.53–57 The form of any par-
FIG. 7. Pollution and loss of zebrafish habitat. Sitesshown here are in Meghalaya. (A) Runoff from a limequarry (arrowheads) joins with clean water (arrow) at theDukan river (site 18, immediately downstream of Fig. 4B).While zebrafish were found upstream of the confluence,no fish or other species were found in water downstream.(B) Coal mining and runoff from piles of coal (arrow-heads) at a site that appeared otherwise suitable for ze-brafish. No living organisms were found in the water atthis location. (C) Streams serve many purposes includingclothes washing. Numerous streams showed evidence ofdetergent use or other chemical or waste contamination.Here, otherwise prime zebrafish habitat, where thisspecies was found within the past 10 years, has been de-nuded of zebrafish and other fauna.
ticular pigment pattern likely reflects interac-tions among these biotic factors (e.g., conspic-uousness to mates vs. predators) as well as theabiotic environment (e.g., ambient light levelsand colors).58,59 The distinctive stripes of ze-brafish presumably reflect the relative impor-tance of such selective factors, and should be in-terpretable with additional reference to thevisual ecology of these fish. In turn, the dis-parate spots, bars, and uniform pigment pat-terns of closely related danios may reflectspecies differences in the relative importance ofthese factors.14,15,60 Similarly, interspecific andintraspecific variation in the lateral line sensorysystem might reflect differences in currentspeeds and predation regimes.21,61–64 Finally,variation in jaws and teeth may reflect differentrequirements for capturing and handling par-ticular kinds of prey.16,65 In this regard it willbe especially interesting to see how competitionamong danios and other syntopic species mayhave shaped the evolutionary history of thesetraits. As more is learned about zebrafish nat-ural history, such insights can be combinedwith developmental and genetic tools availablefor zebrafish, to allow studies spanning levelsof organization from the species level, to selec-tion within populations, to form and variationin form among individuals, to the cell behav-iors and gene activities underlying these forms.
Even limited field observations such as oursalso have implications for research in the labo-ratory. For example, the range of temperaturesin which we found zebrafish suggests that lab-oratory screens for temperature-sensitive mu-tant alleles66–69 with appropriate stocks couldexploit even wider temperature ranges than aretypically used. This thermal range also raisesquestions of how such extremes are toleratedphysiologically. Our finding that zebrafish ap-pear to breed in silt-bottomed, well-vegetatedpools suggests that spawning some “difficult”stocks, particularly if recently wild-caught,might be facilitated by conditions that mimicthose in the field. Finally, our observations sug-gest that historical anthropogenic factors, suchas converting natural water bodies to rice cul-tivation, should be considered when interpret-ing zebrafish ecology and behavior; more re-cent anthropogenic factors, such as habitatdegradation by industrial and other pollutants,
should be seen as lending a certain urgency tofurther studies of zebrafish natural history.
Given the importance of natural zebrafishpopulations, both for their own sake and for research, we suggest a greater emphasis ondocumentation and preservation. Minimally,future field studies should provide GPS coor-dinates and other detailed locality data, suchas distances to nearest town centers or otherlandmarks. This information is critical for lon-gitudinal analyses of populations and habitats,but is lacking from most publications to date.Likewise, population isolates of zebrafishshould receive specific designations, by anal-ogy with nomenclature conventions for thenaming of mutant lines, and the salient datashould be deposited in publicly accessible data-bases such as the Zebrafish Information Net-work. When possible, living fish or DNAshould be made available to the research com-munity through the Zebrafish International Re-sources Center or through other mechanisms.
ACKNOWLEDGMENTS
We thank local fisherman from throughoutour survey region for their assistance and hos-pitality as well as M. Mills, M. Cooper and twoanonymous referees for comments on the man-uscript. Supported by University of Washing-ton set-up funds and National Science Foun-dation IOB-0541733 (to author DMP).
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ENGESZER ET AL.38
AP
PE
ND
IX.
HIS
TO
RIC
AL
CO
LL
EC
TIO
NS
OF
ZE
BR
AFI
SHIN
IND
IAA
ND
NE
IGH
BO
RIN
GC
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NT
RIE
SSI
NC
E18
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alog
num
ber
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lect
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lity
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ntry
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iaB
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rsha
ll A
1983
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ne R
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iaC
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erre
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1930
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ram
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tral
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1940
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rive
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ihar
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teIn
dia
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186
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erts
TR
1975
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taw
an V
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y, i
nclu
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ri K
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nd s
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ley,
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abar
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epal
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348
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erts
TR
1975
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taw
an V
alle
y, 1
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iles
wes
t of
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anga
rN
epal
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S 62
038
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erts
TR
1985
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nata
ka, N
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f M
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eIn
dia
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a SL
1938
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impo
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and
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guri
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aiIn
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286
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s D
1996
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flue
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of 3
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ers
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udha
r, B
ahun
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alR
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dia
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s A
1998
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1998
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, Wes
t A
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, Kha
si H
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MH
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n M
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hang
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ka H
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dia
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lect
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ig. 1
as
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m o
n-lin
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atab
ases
. Spe
cim
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wer
e or
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ally
cla
ssif
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as
zebr
afis
h, D
. rer
io, a
nd w
ere
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ical
ly r
e-ex
amin
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re.
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NH
, Bri
tish
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eum
of
Nat
ural
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tory
; CA
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alif
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cad
emy
of S
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ctio
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ow h
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at
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cad
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s; K
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RM
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, Zoo
logi
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eum
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burg
; ZSI
, Zoo
logi
cal
Surv
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f In
dia
. GPS
co-
ord
inat
es o
r th
eir
appr
oxim
atio
ns a
re a
vaila
ble
thro
ugh
the
onlin
e d
atab
ases
for
eac
h co
llect
ion.
AP
PE
ND
IX.
HIS
TO
RIC
AL
CO
LL
EC
TIO
NS
OF
ZE
BR
AFI
SHIN
IND
IAA
ND
NE
IGH
BO
RIN
GC
OU
NT
RIE
SSI
NC
E18
68a
(CO
NT.)
Cat
alog
num
ber
Col
lect
orY
ear
Loca
lity
Cou
ntry