Zebrafish in studies of human health and disease (Monte Westerfield)

8/20/2019 Zebrafish in studies of human health and disease (Monte Westerfield)

1/36

Glktc Rcstcrefcio, Skfvcrsfty le Lrcblk, Cubckc, SQ@

Qplkslrco dy thc Leefac le thc Ofrcatlr K`tflkì Fkstftutcs le Hcìth,thc K`tflkì Hug`k Bcklgc Tcsc`rah Fkstftutc,

thc K`tflkì Fkstftutc le Ahfio Hcìth & Ocvcilpgckt,

thc K`tflkì Fkstftutc lk Ocèkcss & Lthcr Alggukfa`tflk Ofslrocrs,thc K`tflkì Cyc Fkstftutc,thc Sshcr 0E Alii`dlr`tfvc, ̀ ko thc Gcb`k ̀ ko Ufsflk elr ̀ Aurc Eluko`tflks

]cdrèfsh fk stuofcs le

hug`k hcìth `ko ofsc`sc

[email protected]


2/36

• Cxpcrfgcktì òv`kt`bcs le zcdrèfsh

• ]cdrèfsh `s glocis le hug`k ofsc`sc

• Bckc coftfkb tl bckcr`tc prcafsc glocis le

hug`k bckltypcs

]cdrèfsh fk stuofcs le hug`k hcìth `ko ofsc`sc


3/36

08 hlur zcdr`ffsh

48 o`y hug`k

Ucrtcdr`tc cgdryls


4/36

Qtuoy ocvcilpgckt ofrcatiy fktr`ksp`rckt cgdryls

(J`ristrlg & J`kc)


5/36

acii-spcafefa prlgltcr BE^_li4 _li4_li4jft

Fkmcat fktl zcdr`efsh

Tcplrtcr alkstruats & hfbhiyceefafckt tr`ksbckcsfs

Qarcck elr rcsauc @ss`ys= LJT, CTB, t`p tcst, ̀ plptlsfs,cta.

Glocis elr tr`ksi`tflk`i stuofcs


6/36

Qtuoy physflilby ofrcatiy


7/36

(Rfijfkslk i`dlr`tlry)


8/36

Qtuoy dillo eilw ofrcatiy fk ifvc `kfg`is

(Q`ktlrl i`dlr`tlry)


9/36

R`tah ocvcilpgckt ofrcatiy fk ifvc `kfg`i

(Ifkj i`dlr`tlry)


10/36

Algpicx dch`vflrs - prcy a`pturc

(Ckbcrt i`dlr`tlry)


11/36

(Jciicr & @hrcks, 4>01)

Qtuoy `atfvfty le cvcry kcurlk fk thcdr`fk sfguit`kclusiy


12/36

Hfbh-thrlubhput lptfa`i sarcckfkb

(^`rol-G`rtfk ct `i., 4>0>)


13/36

Rfio-typc

Gut`kt

kl t`fi (kti )

(Jfggci i`dlr`tlry)

Dloy `xfs ocvcilpgckt


14/36

Rfio-typc

Gut`kt

.

Acrcdciiug

`acrcdcii`r (`ac )

Dr`fk ocvcilpgckt

(Kùssicfk-Ulih`ro i`dlr`tlry)


15/36

Klrg`i Ayailps gut`kt

Cyc ocvcilpgckt

(Jfggci i`dlr`tlry)


16/36

_hlus`kos le gut`kts- ` - f =

\-

"

"\\c

:eef

r'trff..-'=.

, . . ' e ' .

t ' = , '

0ps5

-{s..$

-=mt**i '

"Y{-{*Q*r

iE

\\7

-r:f1--

eef

t.*

."* *Y..

I

ry"u.iQr.

tqy

*

"

.0,-"=

. ,.

"f-".*l*

t

rgeefr*&c

ruru`:#

.i=****:*cbgl*


17/36

Jklaj lut le `ii prltcfk alofkb bckcs


18/36

Tcvcrsc bckctfa tcahklilby

ATFQ^Ts - Aiustcrco Tcbui`riyFktcrsp`aco Qhlrt ^`ifkorlgfa Tcpc`ts

GLT^HLIFKLQ- `ktfscksc lifblkuaicltfocs


19/36

Glrphlifkls vs bckc coftfkb

(Jlj ct ì., 4>03)

Elr 8>% le bckcs, glrphlifkl phckltypcs

`rc klt ldscrvco fk gut`kts

Glrphlifkl ATFQ^T/_@ICK@ov`kt`bcs Ofsòv`kt`bcs

Qtuoy ofrcatiy fkfkmcatco `kfgìs

Qtuoy fk st`dic ifkcs

C`sy tl usc Tcqufrcs drccofkb &

bckltypfkbDilajs g`tcrkìgcss`bc

Tcqufrcs g`tcrkì zybltfagut`kts tl dilaj g`tcrkìprlouats

Ofsòv`kt`bcs @ov`kt`bcs

Ofiutco lvcr tfgc ‑ifgftco tl c`riy

ocvcilpgcktì st`bcs

Bckctfaìiy st`dic ‑ a`kstuoy `t ìi ocvcilpgcktì

st`bcs fkaiuofkb òuits

Fkouacs p31ocpckockt `plptlsfs

Ilw tlxfafty

Lee t`rbct ceecats Hfbhiy spcafefa


20/36

Glrphlifkls vs bckc coftfkb

(Jlj ct ì., 4>03)

Elr 8>% le bckcs, glrphlifkl phckltypcs

`rc klt ldscrvco fk gut`kts

Glrphlifkl ATFQ^T/_@ICK@ov`kt`bcs Ofsòv`kt`bcs

Qtuoy ofrcatiy fkfkmcatco `kfgìs

Qtuoy fk st`dic ifkcs

C`sy tl usc Tcqufrcs drccofkb &

bckltypfkbDilajs g`tcrkìgcss`bc

Tcqufrcs g`tcrkì zybltfagut`kts tl dilaj g`tcrkìprlouats

Ofsòv`kt`bcs @ov`kt`bcs

Ofiutco lvcr tfgc ‑ifgftco tl c`riy

ocvcilpgcktì st`bcs

Bckctfaìiy st`dic ‑ a`kstuoy `t ìi ocvcilpgcktì

st`bcs fkaiuofkb òuits

Fkouacs p31ocpckockt `plptlsfs

Ilw tlxfafty

Lee t`rbct ceecats Hfbhiy spcafefa


21/36

Bckc coftfkb fs dcalgfkb ` prfg`rybckctfa tcahklilby fk zcdr`efsh rcsc`rah

_@ICKs

ATFQ^Ts

Glrphlifkls (x >.4)

^ u d i f a ` t f l k s

>

0>

4>

1>

?>

3>

Vc`r

4>0> 4>00 4>04 4>01 4>0? 4>03


22/36




hug`k bckltypcs



23/36

zcdr`efsh eiy

hug`k

zcdr`efsh


24/36

@autc ròf`tflk sykorlgc

@bc-rci`tco gàui`r ocbckcr`tflk

@ialhlifsg

@ialhlifa ifvcr ofsc`sc @izhcfgcrs ofsc`sc @klphthìgf`

@kxfcty ofslrocrs

@thcrlsaicrlsfs @utfsg

@utlslgì olgfk`kt hc`rfkb ofsc`sc @xckecio-Tfcbcr sykorlgc

D`roct-Dfcoi sykorlgc

D`rth sykorlgcDfauspfo `lrtfa vìvc ofsc`sc

Dfif`ry ̀ trcsf`

A`kacrAlilrcatì a`kacr

Hcò `ko kcaj squ`glus acii a`rafklg`Biflg`

Fkv`sfvc a`rafklgÌCL^@TO sykorlgc

Icujcgf`

Ifvcr a`kacrIygphldi`stfa icujcgf`

Gci`klg`Gylilbcklus icucjgf`

Kcurldi`stlg`

^`karc`tfa a`kacrTh`dolgyls`ralg`

Acktrlkuaic`r gylp`thfcsAcrcdrì a`vcrklus gìelrg`tflks

Ahlicr`

Ahrlkfa br`kuilg`tlus ofsc`scAhrlkfa jfokcy ofsc`sc

Afraui`tlry ofsc`scsAliftfs

Alkbckftì a`t`ràt

Alkbckftì biyalsiy`tflk ofsc`scsAlrc dfkofkb eàtlr icujcgf`

Alrkcì oystrlphfcs

Ar`kfì eàfì ofsc`scs

Arlhks ofsc`sc

Autfs i`xÀystfa efdrlsfsOci`yco pudcrty

Of`dctcs

Of`glko Diàje`k `kcgfÒuahckkc gusaui`r oystrlphy

Cko st`bc rckì ofsc`scCgphyscg`

Cpficpsy

Cxtrfksfa ̀ sthg`Cyc ofsc`scs

Eàflsa`puilhugcrì gusaui`r oystrlphy

E`kalkf `kcgfÈ`tty ifvcr ofsc`scEctì ìalhli sykorlgc

Elaì scbgcktì bilgcruilsaicrlsfs

Er`scr sykorlgcB`kbiflsfoc sfìfo`sc ocefafckay ofsc`sc

B@^L prlbcrlfo sykorlgcBiùalg`

Bifldi`stlg`

Hc`rt ofsc`sc @trfì efdrfii`tflk

A`rofà ̀ rrhythgf`

A`rofà alkouatflk ofsc`scsA`roflgcbìy

Alkbcstfvc hc`rt e`fiurcHypcrtrlphfa a`roflgylp`thy

Quoock a`rofà `rrcstHcp`tfa stc`tlsfs

Hcrcoft`ry rctfkì ocbckcr`tflks

Hfrsahsprukb ofsc`scHFU@FOQ

Hlit Lr`g sykorlgcHypcr`gglkcgf`

Hypcrtcksflk

Fkei`gg`tlry dlwci ofsc`sc

Fktràr`kfì ̀ kcurysg

Mludcrt sykorlgc

Muvckfic hyorlacphìusJfokcy ofsc`scsIlkb P_ sykorlgc

Iyslslgì ofsc`sc gualifpfolsfs FF

Gfarlphth`gf`Gfoeàfì aicet ofsc`sc

Guitfpic saicrlsfsGualifpfolsfs typc FU

Gusaui`r oystrlphy

Gyltlkfa oystrlphyKcphrlklphthfsfs

Klksykorlgfa aicet ifp `ko pì`tc

Kllk`k sykorlgcLdcsfty

Lrì aicet ofsc`scsLstclplrlsfs

^`rjfkslk ofsc`sc^liyaystfa jfokcy ofsc`sc

^scuolx`kthlg` ci`stfaug

Tceràtlry ̀ kcgf`Qahfzlphrckf`

QaliflsfsQjcictì ofsc`scs

Qiccp ofslrocrs

Qpfkì alro rcbckcr`tflkQpfkì gusaui`r `trlphy

Qtrljc_rcàhcr Aliifks sykorlgc

_udcrauilsfs

Sshcr sykorlgcSvcì alildlg`

U`saui r̀ thrlgdlsfsvlk Rfiicdr`ko ofsc`sc

Hug`k ofsc`scs stuofco wfth zcdrèfsh glocis

(KFH eukoco br`kts, 4>01)


25/36



@ialhlifsg


@kxfcty ofslrocrs





Dfif`ry ̀ trcsf`




Icujcgf`



Kcurldi`stlg`



Ahlicr`



Alkbckftì a`t`ràt


Alrkcì oystrlphfcs


Arlhks ofsc`sc


Of`dctcs



Cpficpsy







Bifldi`stlg`









Hypcrtcksflk



Mludcrt sykorlgc





Gusaui`r oystrlphy









Qiccp ofslrocrs



_udcrauilsfs





@izhcfgcrs Ofsc`sc


26/36


27/36



@ialhlifsg


@kxfcty ofslrocrs





Dfif`ry ̀ trcsf`




Icujcgf`



Kcurldi`stlg`



Ahlicr`



Alkbckftì a`t`ràt


Alrkcì oystrlphfcs


Arlhks ofsc`sc


Of`dctcs



Cpficpsy







Bifldi`stlg`









Hypcrtcksflk



Mludcrt sykorlgc





Gusaui`r oystrlphy









Qiccp ofslrocrs



_udcrauilsfs





A`kacrs


28/36



@ialhlifsg


@kxfcty ofslrocrs





Dfif`ry ̀ trcsf`




Icujcgf`



Kcurldi`stlg`



Ahlicr`



Alkbckftì a`t`ràt


Alrkcì oystrlphfcs


Arlhks ofsc`sc


Of`dctcs



Cpficpsy







Bifldi`stlg`









Hypcrtcksflk



Mludcrt sykorlgc





Gusaui`r oystrlphy









Qiccp ofslrocrs



_udcrauilsfs





Ldcsfty


29/36



@ialhlifsg


@kxfcty ofslrocrs





Dfif`ry ̀ trcsf`




Icujcgf`



Kcurldi`stlg`



Ahlicr`



Alkbckftì a`t`ràt


Alrkcì oystrlphfcs


Arlhks ofsc`sc


Of`dctcs



Cpficpsy







Bifldi`stlg`









Hypcrtcksflk



Mludcrt sykorlgc





Gusaui`r oystrlphy









Qiccp ofslrocrs



_udcrauilsfs





Qahfzlphrckf`


30/36



@ialhlifsg


@kxfcty ofslrocrs





Dfif`ry ̀ trcsf`




Icujcgf`



Kcurldi`stlg`



Ahlicr`



Alkbckftì a`t`ràt


Alrkcì oystrlphfcs


Arlhks ofsc`sc


Of`dctcs



Cpficpsy







Bifldi`stlg`









Hypcrtcksflk



Mludcrt sykorlgc





Gusaui`r oystrlphy









Qiccp ofslrocrs



_udcrauilsfs





Sshcr sykorlgc


31/36

Sshcr sykorlgc - thc icòfkb aùsc le ocèdifkokcss

• ^rcvìckac ! 0 pcr 5,>>> dfrths fk thc SQ

(glrc algglk th`k @IQ lr Huktfkbtlk‖s Ofsc`sc)

• Alkbckftì ocèkcss (~?% le ocè h`vc Sshcr)

Qckslrfkcurì hc`rfkb ilssUcstfdui`r oyseukatflk

• Tctfkftfs pfbgcktlsÌlss le rlo phltlrcacptlrs

^rlbrcssfvc tukkci vfsflk `s alkcs ofc


32/36

T4?3X gut`tflk truka`tcs thc ÂOH03 prltcfkaùsfkb Sshcr sykorlgc typc 0E

T4?3X fs algglk fk thc @shjck`zf Mcwfsh alggukfty- àalukts elr ~53% le Sshcr 0 p`tfckts


33/36

Cxlk sjfppfkb `s str`tcby elr thcr`py

020 dp(32`` - fk er`gc)

04 Jd 8> Jd

sftc le prcg`turc tcrgfk`tflk

alolk fk T4?3X p`tfckts

`sl0 `sl4

C2 C8 C6

C2 C6

Cxlk 8 ocictco erlg tr`ksarfpt

^AOH03 prc-gTK@

^AOH03 g`turc gTK@


34/36

Cxlk sjfppfkb `s str`tcby elr thcr`py

04 Jd 8> Jd

T4?3X

C2 C6C8

]cdr`efsh bckc

^rcafsc gloci lehug`k gut`tflk

Hug`k cxlk +

Ei`kjfkb fktrlk scquckacs

ATFQ^T

C8

04 Jd 8> Jd

C2 C6C8

T4?3X

`sl0 `sl4

C8

]cdr`efsh hlglilby`rgs

+


35/36




hug`k bckltypcs - 122 bckcs t`rbctco sfkac 4>00



36/36

Glktc Rcstcrefcio, Skfvcrsfty le Lrcblk, Cubckc, SQ@

Qplkslrco dy thc Leefac le thc Ofrcatlr K`tflkì Fkstftutcs le Hcìth,thc K`tflkì Hug`k Bcklgc Tcsc`rah Fkstftutc,

thc K`tflkì Fkstftutc le Ahfio Hcìth & Ocvcilpgckt,thc K`tflkì Fkstftutc lk Ocèkcss & Lthcr Alggukfa`tflk Ofslrocrs,

thc K`tflkì Cyc Fkstftutc,thc Sshcr 0E Alii`dlr`tfvc, ̀ ko thc Gcb`k ̀ ko Ufsflk elr ̀ Aurc Eluko`tflks

]cdrèfsh fk stuofcs le

hug`k hcìth `ko ofsc`sc

[email protected]

Documents

Zebrafish in studies of human health and disease (Monte Westerfield)