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Your new alternative to LC-MS
MASS SPEC COMPATIBLE CHARGE VARIANT ANALYSIS
Many current mAb characterization techniques either provide high-resolution charge variant separations
or mass spec data. Not both. This makes identification of mAb variants difficult.
No more either/orZipChip® can perform high-resolution charge variant separations of intact antibodies backed by the power
of Native-MS characterization. In a single run you can get mass information, assess charge heterogeneity,
characterize glycoforms, identify basic and acidic variants, and more. The added layer of mass spec
information means you can finally pinpoint the identity of each peak.
Get the unfair advantageWhen compared to traditional methods for charge variant analysis of antibodies, ZipChip separates more
and allows mass spec identification for each charge variant.
Charge Variant Separation
MS CompatibleCharge Variant
MassesCharge Variant ID
ZipChip HHHHH HHHHH HHHHH HHHHH
Isoelectric focusing HHHHH HH
CE (UV) HHHHH HH
RPLC-MS H HHHHH HH HH
APPLICATION SNAPSHOT
Charge Variant Analysis
Z I P C H I P I S F O R R E S E A R C H U S E O N LY ( R U O )
Charge variant analysis of NIST mAb
Charge variant analysis of NIST mAb stressed at pH 8 for 72 hours
Kit Type: Native Antibody
Chip Type: ZipChip HRN
Sample Prep: Dilution from formulation. Buffer exchange may be necessary
MS Type: Thermo Scientific Orbitraps with Biopharma or EMR options
Native Mass Spectrum
+26 Charge State
7.0 7.5 8.0 8.5 9.0 9.5 10.0 10.5 11.0Time (min)
05
101520253035404550556065707580859095
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ecnadnubA evitaleR
Main
Basic 2
Basic 1
Basic 2Deamidated Main
Deamidated
Main
5680 5700 5720 5740 5760m/z
0102030405060708090100
Rel
ativ
e Ab
unda
nce
4000 4500 5000 5500 6000 6500 7000 7500 8000m/z
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101520253035404550556065707580859095
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e Ab
unda
nce
7.0 7.5 8.0 8.5 9.0 9.5 10.0 10.5Time (min)
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ecnadnubA evitaleR
Main
Basic 2
Basic 1 Acidic
Charge Variant Analysis
www.908devices.com
Z I P C H I P I S F O R R E S E A R C H U S E O N LY Zip Chip is subject to export controls including those of the Export Administration Regulations of the U.S. Department of Commerce, which may restrict or require licenses for the export of product from the United States and their re-export to and from other countries. Patented technology www.908devices/patents © 2019 908 Devices
WHIZ THROUGH INTACT MASS
APPLICATION SNAPSHOT
Intact Mass
Z I P C H I P I S F O R R E S E A R C H U S E O N LY ( R U O )
Intact mass analysis is routinely used to characterize proteins and provide sequence confirmation. The
system used for this analysis should be high-throughput, simple, and tolerant of many different sample
matrices so that sample prep is easy, and data is generated quickly.
Accelerate intact analysisZipChip® provides a fast, accurate, and flexible approach to intact mass analysis. Rapidly screen for the
ultimate in high-throughput analysis, or tailor the method to suit a flexible mAb characterization approach
without having to change conditions or instrumentation. Queue it all up, ignore your salt and polymeric
buffer components, and hit go.
Get the unfair advantageWhen compared to traditional methods for intact mass determination, ZipChip exploits the advantages
of microfluidic CE-MS to provide a sensitive, very simple, highly flexible approach to getting the answer
you need in less time with less hassle.
Speed of Sample Prep
Speed of Analysis
Detergents
Allowed
Compatible w/ Formulation Buffer
Compounds
ZipChip MS HHHHH HHHHH HHHHH HHHHH
MALDI-TOF H HHHHH HH H
RP-LC/MS HHHH HHH H HHHH
CE-MS HH HH — HH
Intact Mass
www.908devices.com
Z I P C H I P I S F O R R E S E A R C H U S E O N LY Zip Chip is subject to export controls including those of the Export Administration Regulations of the U.S. Department of Commerce, which may restrict or require licenses for the export of product from the United States and their re-export to and from other countries. Patented technology www.908devices/patents © 2019 908 Devices
3140 3160 3180m/z
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Rel
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High Throughput Intact Mass
Run Times: Less than 1 minute
MS Types: Thermo QE-HF and Fusion Lumos
Chip Type: ZipChip HS
Kit Type: ZipChip Peptide Kit
High Res Data
NIST mAbZipChip HS - 1000 V/cm Peptide BGE
NIST mAbZipChip HR - 500 V/cm
Peptide BGE
Standard Intact Mass
Run Times: Less than 3 minutes
MS Types: All mass spectrometers capable of intact mass analysis
Chip Type: ZipChip HR
Kit Type: ZipChip Peptide Kit
0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2 3.4Time (min)
0102030405060708090
100
0102030405060708090
100
ecnadnubA evitaleR
0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2 3.4Time (min)
2500 3000 3500 4000 4500m/z
0102030405060708090
100
Rel
ativ
e Ab
unda
nce
2500 3000 3500 4000m/z
0102030405060708090
100
ecnadnubA evitaleR
3140 3150 3160 3170 3180m/z
0102030405060708090
100
Rel
ativ
e Ab
unda
nce High Res
Data
ACCELERATE CHARGE VARIANT ANALYSIS
APPLICATION SNAPSHOT
High Throughput Charge Variant Analysis
Z I P C H I P I S F O R R E S E A R C H U S E O N LY ( R U O )
Charge variant analysis is routinely used to characterize proteins and provide critical information
on the charge heterogeneity of recombinant protein. The system used for this analysis should be high
throughput, simple, and able to tolerant many different sample matrices so that sample prep is easy, and
data is generated quickly.
Accelerate charge variant analysisZipChip® provides a fast, accurate, and flexible approach to charge variant analysis. Rapidly screen for
the ultimate in high-throughput analysis, or tailor the method to suit a flexible mAb characterization
approach without having to change conditions or instrumentation. Queue it all up, ignore your salt and
polymeric buffer components, and hit go.
Get the unfair advantageCurrent methodologies for charge variant analysis are typically done with conventional capillary-based
CE systems using UV detection. ZipChip can not only perform native charge variant analysis coupled to
high resolution mass spectrometry, but now has the added capability of choosing between high resolution
OR high throughput approaches.
Intact mAb ChargeVariant Separations
Native ESI-MS Spectra
Superior SpeedSuperior Separation
Resolution
HSN
HRN
High Throughput Charge Variant Analysis
www.908devices.com
Z I P C H I P I S F O R R E S E A R C H U S E O N LY Zip Chip is subject to export controls including those of the Export Administration Regulations of the U.S. Department of Commerce, which may restrict or require licenses for the export of product from the United States and their re-export to and from other countries. Patented technology www.908devices/patents © 2019 908 Devices
High Throughput Charge Variant Analysis
Run Times: As fast as 2 minutes
Chip Type: ZipChip HSN
Kit Type: Native Antibodies Kit
Standard Charge Variant Analysis
Run Times: About 10 minutes (molecule dependent)
Chip Type: ZipChip HRN
Kit Type: Native Antibodies Kit
ZipChip® can be used to perform an intact charge variant assay under native-ESI conditions. These
conditions generate an intact mass spectrum suitable for characterizing the intact mass and major
(charged) modifications to the protein, including deamidation. By changing the type of chip used, the
speed of the analysis can be easily tailored to the best suit the experiment and MS instrument used.
NIST mAbZipChip HSN – 1000 V/cm
NIST mAbZipChip HRN – 500 V/cm
GIVE YOUR WORKFLOW SOME ZIP
Analysis of amino acids during spent media analysis can provide important metabolic information about
the cell culture’s health, but it is difficult to do using conventional techniques. Current methods for cell
culture media analysis are slow, involve complicated sample preparation (e.g., derivatization), and can be
expensive.
Give your workflow some zipCell culture analysis is easy and fast with ZipChip®. Samples are prepared through a simple dilution and
there’s no labeling or derivatization. Small polar analyte assays take as little as 2 minutes. Add internal
standards for full quantitation on a variety of analytes.
Get the unfair advantageWhen compared to traditional methods for cell culture analysis of antibodies, ZipChip provides the only
dilute-and-shoot, label-free analysis that is fast and simple to use.
Simple Sample Prep Label Free Fast Analysis
ZipChip HHHHH HHHHH HHHHH
HPLC/UPLC H H HH
LC-MS HHH HHH HHH
GC H H H
Z I P C H I P I S F O R R E S E A R C H U S E O N LY ( R U O )
APPLICATION SNAPSHOT
Cell Culture Analysis
ZipChip separation of spent growth media
The amino acids migrate through the chip in less than 3 minutes. Isomers, such as isoleucine and leucine, are resolved and detected as separate peaks for confident identification and quantitation.
RT :0.00 - 3.00
0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8Time (min)
05
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ecnadnubA evitaleR
132.10116.07
132.10147.11
147.08120.98175.12 133.06
120.07165.09148.06192.11
134.0494.06105.00 79.04 132.07
Isoleucine
LeucineRT :1.33 - 1.62
1.35 1.40 1.45 1.50 1.55 1.60Time (min)
0
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0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
10.0
)M
m( noitartnecnoC dicA oni
mA
Valine Methionine Asparagine
Characterize the components in your growth media, or collect timepoints during cell culture to track nutrient metabolism and optimize growth.
Cell Culture Analysis
www.908devices.com
Z I P C H I P I S F O R R E S E A R C H U S E O N LY Zip Chip is subject to export controls including those of the Export Administration Regulations of the U.S. Department of Commerce, which may restrict or require licenses for the export of product from the United States and their re-export to and from other countries. Patented technology www.908devices/patents © 2019 908 Devices
SPECIFICATIONS
Your mass spec’s sidekick.
H A R D W A R E S P E C S H E E T
Put Your Mass Into It
Specifications
Sample Format Vials or standard well plates (47 mm max height)
Sample Capacity Two sample trays that hold 48 vial racks or 96-well plates
Sample Tray Cooling• Integrated Peltier Cooling • Range: 4 ºC to ambient – 3 ºC (at 80% relative humidity and 25 ºC ambient temperature)
Dimensions 14 in (h) x 12 in (w) x 22 in (d)
Weight 46.3 lbs
Power Requirements 110/240V
Specifications
Software• Standalone ZipChip acquisition software • Integrates with your MS software
Dimensions 8 in (h) x 8 in (w) x 11 in (d)
Weight 16 lbs
Power Requirements 110/240V
Laser Type Class 1 Laser Product
MS Compatibility
Thermo Scientific
• Orbitrap Exactive and Q Exactive™ Series • Orbitrap Fusion Lumos Tribrid• Orbitrap Exploris 480• Orbitrap Eclipse Tribrid LTQ and Orbitrap Elite Series• TSQ2 Series Triple Quads
SCIEX• QTRAP® 6500+, 6500, 5500, 4500 • Triple Quad 6500+, 6500, 5500, 4500, 3500 • TripleTOF 6600+, 6600, 5600
BRUKER
• compact™
• impact™
• maXis™
• timsTOF (pro)
The Interface
The compact interface seamlessly integrates all fluidics and electronics. Just clip it on your mass spec and off you go!
Optional Autosampler
Achieve unattended sample runs from sample vials or 96-well plates. The platform allows you to choreograph sample runs using ZipChip software.
ZipChip is for Research Use Only (RUO)
H A R D WA R E S P E C S H E E T
ZipChip is subject to export controls including those of the Export Administration Regulations of the U.S. Department of Commerce, which may restrict or require licenses for the export of product from the United States and their re-export to and from other countries. Patented technology www.908devices/patents. © 2020 908 Devices.
ZipChip [email protected] | www.908devices.com
Put Your Mass Into It
Consumables to simplify your workflow.
C O N S U M A B L E S S P E C S H E E T
ACTUAL SIZE
C O N S U M A B L E S S P E C S H E E T
Capacity 625 samples per kit
Contents5x 125 mL bottles of background electrolyte 5x 15 mL bottles of sample diluent
UsageCompatible with manual and autosampler ZipChip interface types.
Storage Conditions Room temperature, 10-40 ºC
The Kits
Premixed background electrolyte and sample diluent reduce preparation time for your samples. With ZipChip you just dilute and shoot!
Available Kits
Native Antibodies: Excellent charge variant separation of antibodies and other constructs to generate native spectra.
Charge Variant TOF: Formulated to provide excellent charge variant separations and MS sensitivity for antibodies and other constructs on time-of-flight mass spectrometers.
Intact Antibody: Fast, high sensitivity separations of intact antibodies without full denaturation.
Peptides: Fast, efficient separations of protein digests and peptides utilizing low pH.
Metabolites: Streamlined sample prep and high-speed analysis for any size molecule in denaturing conditions at low pH.
The Chips
Choose from these chip options to optimize your workflow. Each chip can be used for up to 125 injections. Available Chips
HR: Our standard High Resolution chip for applications demanding maximum separation performance for analytes such as large molecules and many more. To be paired with the Intact Antibody Kit, Peptides Kit, or Metabolites Kit.
HS: Our standard High Speed chip to give you the fastest separations for applications such as small molecules, metabolites and other applications where throughput is critical. To be paired with the Intact Antibody Kit, Peptides Kit, or Metabolites Kit.
HRN: A twist on our HR chip that provides ultimate performance for native protein analysis. To be paired with the Native Antibodies Kit and Charge Variant TOF Kit.
HSN: The High Speed version of our native chip for high throughput analysis of proteins analyzed under native conditions. To be paired with the Native Antibodies Kit and Charge Variant TOF Kit.
HRX and HSX: Double the run count of our standard HR and HS chips. Use these chips for up to 250 injections.
ZipChip is subject to export controls including those of the Export Administration Regulations of the U.S. Department of Commerce, which may restrict or require licenses for the export of product from the United States and their re-export to and from other countries. Patented technology www.908devices/patents. © 2020 908 Devices.
ZipChip [email protected] | www.908devices.com
ZipChip is for Research Use Only (RUO)