Symposium Schedule TAC Building Auditorium N107

Tuesday June 26th / Optogenetics I

2:00 pm “Conditional Control of Protein Activity Using Optical Dimerizers” Chandra Tucker, PhD

3:00 pm “Acute and Local Manipulations of Plasma Membrane Phosphoinositides” Olof Hagren, PhD

4:00 pm “Peeking and Poking at RhoGTPase Networks in Living Systems” Klaus Hahn , PhD

Wednesday June 27th / Optogenetics II

2:00 pm “Imaging Voltage with Microbial Rhodopsins” Adam Cohen, PhD

3:00 pm “Combining Optogenetics and 2-photon Microscopy to Reveal Inhibitory Control of Dendritic Signaling Chiayu Chiu, PhD

4:00 pm “Do-it-yourself Optogenetics in Freely Behaving Worms” Hang Lu, PhD

5–6:00 pm HAPPY HOUR (each day)

Open Access Equipment & Software TAC 2nd Floor Med Student Teaching Labs

Tuesday – Thursday

Software Walk-in Clinics for Post Acquisition Image Analysis Imaris, Volocity, TissueGnostics

Continued Access to Instrumentation throughout the day (9–5) TAC N221 - N239

Lectures, Practicals & Technical Demos TAC North 2nd Floor / Other Locations

Tuesday June 26th

10:30 am Small group practical with optogenetic fluorophore (group I)

11:30 am CYTOO micropatterned substrates CYTOO presentation – FMB 201 12:15 am “Emerging camera technologies: a guide to CCDs and sCMOS cameras” Hamamatsu – FMB 201

1:00 pm Demo of Leica Ti:Sapphire STED microscope Leica – SHM IE wing

1:00 pm Spinning Disk Demonstration BioVision CARV II – TAC N239

Wednesday June 27th 10:30 am Small group practical with optogenetic fluorophore (group II)

11:00 am Columbus software for remote high volume image management Perkin Elmer TAC N209

12:30 pm Demo of Leica GSD ground state depletion microscope Leica – TAC N221

1:00 pm “Basic fluorescence widefield deconstructed: Understanding the Building Blocks of Fluorescence Microscopes “ Interactive Nikon session – TAC N223

Thursday June 28th 10:30 am Small group practical with optogenetic fluorophore (group III)

1:00 pm “Latest Confocal Technologies from Leica” Leica – TAC N221

1:00 pm Demo of InjectMan programmable micromanipulator Eppendorf – TAC N223

2:00 pm TIRF demonstration Leica – TAC N221

2:30 pm TisueGnostics integrated “image to data” system TissueGnostics presentation – TAC N239

3:30 pm Demo of two-photon laser scanning microscope LaVision BioTec demo – TAC S614

Supported by the Yale Rheumatic Disease Research Core Center & YSOM Office for Academic & Scientific Affairs. Please contact organizers if interested in bringing your own live samples.

Co-organizers: Ann Haberman ann.haberman@yale.edu / Derek Toomre derek.toomre@yale.edu / Joerg Bewersdorf joerg.bewersdorf@yale.edu

Details at www.microscopy.med.yale.edu

Yale Microscopy Workshop

Two-day symposium and three days of open access to state-of-the art instruments 9–6 PM each day

Fully functional microscopes representing all modalities – confocal / two-photon LSM / spinning- disk Post acquisition Image analysis / Demonstrations and practicals

Free registration online or on site in the lobby of the TAC Building

June 26 – 28, 2012300 Cedar StreetNew Haven, CT

OPTOGENETICS SYMPOSIUM THEME

NIAMSNational Institute of Arthritis and Musculoskeletal and Skin Diseases

KAVLISPONSORED BY THE

INSTITUTE AT YALE