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Xray crystallography
Lasse Mårtensson, IFM-Chemistry
Ribosome
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Diffraction pattern from a protein obtained by xray crystallography
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Electrondensity map
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• Requirements:
• Pure protein of high concentration 10-50 mg/ml AND…
• Protein crystals
How to obtain protein crystals??
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Crystallization robot
Terms used in Xray crystallography
• Crystal system
• Spacegroup
• Unit cell
• Assymmetric unit
• Miller indices hkl
• Braggs law
• Ewald sphere
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Ewald sphere with a radius of 1/All lattice points that touch the lattice obey Bragg´s law
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Refinement of the structure
Quality index
R-factor:
Ramachandran plot
