WP7. Radiobiology-KI (B5) - CERN · 7-M4 Effectiveness of another ion species on clonogenic cell survival and modes of cell death. RBE and LET effects m24 Irradiations with a lighter

WP7WP7.Radiobiology-KI (B5)

Medical Radiation PhysicsDepartment of Oncology-PathologyKarolinska InstitutetStockholm, Sweden

16-17th of October 2008

WP7. Milestones and DeliverablesNo. Name Expected date Nature7-D1 Research plan m3 Report-PP

7-M1 Effectiveness of photons on clonogenic cell survival and modes of cell death

m12 Irradiations at CCK, Stockholm, Swedenand modes of cell death Stockholm, Sweden

7-D2 Effects of photon irradiations on cell survival m12 Report-PP

7-M2 Effectiveness of one ion species on clonogenic cell survival and modes of cell death. RBE and LET effects

m24 Irradiations with carbon and or nitrogen ions at TSL,Uppsala Swedeneffects Uppsala, Sweden

7-M3 Effectiveness of the same ion species as above on clonogenic cell survival and modes of cell death. RBE and LET effects

m18 Irradiations with carbon ions at GSI, Darmstadt, Germany

7-D3 Effects of carbon ions on cell survival m18 Report-PP

7-M4 Effectiveness of another ion species on clonogenic cell survival and modes of cell death. RBE and LET effects

m24 Irradiations with a lighter ion at e.g. GANIL, Caen, France and/or HIMAC, Chiba, Japan

7-D4 Effects of a lighter ion on cell survival m24 Report-PP

7-M5 Effectiveness of micro-beams on modes of cell death

m36 Irradiations with micro-beams at the University of Surrey, UK

Annelie E. Meijer 2

7-D5 Effects of micro beams on cell survival m36 Report-PP

7-M6 Finishing thesis and publications m42

7-D6 Publications and thesis m42 Report-PP















Annelie E. Meijer 3




Cell death measurementsl i ll i lclonogenic cell survival

Annelie E. Meijer 4

Cell death measurementsdifferent modes of cell death

Human melanoma cells; AA, exposed to boron ionsHuman SCLC; U-1690

exposed to boron and nitrogen ions

different modes of cell death

Meijer et al. 2005

Human glioma cells; M059J and M059Kexposed to nitrogen ions

Meijer et al. 2008

Holgersson et al. 2003

j















Annelie E. Meijer 6




Cell deathl i ll i lclonogenic cell survival

100

CellsDose(Gy) Colonies PE SF

10-1

100 0 108 106 117 1,10333 1,00000

100 0,5 82 91 88 0,87000 0,78852

10-2

rviv

ing

fract

ion

100 1 85 86 75 0,82000 0,74320

200 2 144 141 151 0,72667 0,65861

1000 5 150 148 158 0 15200 0 13776

10-3

Surv1000 5 150 148 158 0,15200 0,13776

6000 8 127 140 99 0,02033 0,01843

10000 10 60 60 56 0,00587 0,00532

0 2 4 6 8 10 12 14 16 18

10-4

Dose (Gy)

12000 12 18 17 14 0,00136 0,00123

15000 14 12 5 7 0,00053 0,00048

Annelie E. Meijer 7

Dose (Gy)

20000 16 3 2 2 0,00012 0,00011

Edgren et al. 2005

Cell deathi d ti f t ie.g. induction of apoptosis

50

60 1-5 h 24 h 48 h 72 h 144 h

a)

osis

(%)

30

40

216 h

-indu

ced

apop

to

10

20

Rad

iatio

n

0 1 2 3 4 5 6

0

Dose (Gy)

Annelie E. Meijer 8

Meijer et al. 2005















Annelie E. Meijer 9


















Annelie E. Meijer 10


































































































































Documents

WP7. Radiobiology-KI (B5) - CERN · 7-M4 Effectiveness of another ion species on clonogenic cell survival and modes of cell death. RBE and LET effects m24 Irradiations with a lighter