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WormBase -- one Web site, many roles. Caenorhabditis elegans. Diverse data (from 3,739 papers). Prior to July, 2006:. 127 phenotype objects in WormBase. three-tiered organization (specialization_of or generalization_of) redundancy existed between terms - PowerPoint PPT Presentation
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PATO, December 2006
WormBase -- one Web site, many roles WormBase -- one Web site, many roles
PATO, December 2006
Caenorhabditis elegansCaenorhabditis elegans
PATO, December 2006
Diverse data (from 3,739 papers)Diverse data (from 3,739 papers)
1129
834
610 598529 503 479
419351 344 326
278
193150 130 124
85 58 58 57 43 26 20 12
0
200
400
600
800
1000
1200
Expression data
RNAi
Gene function
Sequence change
Transgene
Gene product interactions
Antibody
Gene-gene interactions
Gene-seq, gene name, synonym
Structure correctionMutant phenotype
New allele
Overexpression
Site of action analysisSequence features
Mapping data
Cell (name,function,ablation)
Mosaic analysisStructural info
Protein functions in vitro
Microarray
Covalent modification
SNPs
Functional complementation
PATO, December 2006
Prior to July, 2006:Prior to July, 2006:≈ 127 phenotype objects in WormBase.≈ three-tiered organization (specialization_of or
generalization_of)≈ redundancy existed between terms≈ no phenotype term definitions, references≈ many RNAi experiments annotated to ‘Unclassified’
phenotype term≈ ‘Not’ phenotype associations were not captured≈ Phenotype vocabulary was not used for annotation of
alleles and transgene objects
PATO, December 2006
A controlled and structured A controlled and structured vocabulary for phenotypes:vocabulary for phenotypes:
≈ allows complex data queries, and expedites analysis of genes that act in the same processes or pathways.
≈ helps to integrate a massive array of data from many different sources into a common body of knowledge.
≈ provides the option of linking phenotype data with other data in WormBase or with data from other databases.
≈ facilitates communication within and outside of the C. elegans community
PATO, December 2006
Expansion of the phenotype Expansion of the phenotype ontology, source for term names:ontology, source for term names:
≈ text descriptions in WormBase ≈ free text phenotype descriptions associated with alleles ≈ text associated with RNAi objects annotated to ‘Unclassified’
phenotype
≈ prior phenotype terms in WormBase≈ GO ontology≈ WormBase anatomy ontology≈ Life stage ontology
Term names and synonyms reflect the language of researchers.
PATO, December 2006
The WormBase phenotype ontology The WormBase phenotype ontology is a pre-coordinated ontology:is a pre-coordinated ontology:
1348 terms, ~20% of terms are defined
PATO, December 2006
Current term usage: Current term usage:
1
2
40% used for annotation
60% not associated with an annotation
PATO, December 2006
RNAi-phenotype data:RNAi-phenotype data:
≈ 272,759 total RNAi-phenotype connections≈ 63,439 RNAi experiments≈ 19,692 genes associated with phenotypes
via RNAi experiments:≈ 19,185 genes connected via “Not” associations≈ 4,577 genes connected directly
PATO, December 2006
Allele-phenotype data:Allele-phenotype data:
≈ Most phenotype connections are to knockout alleles (NBP).
≈ Ongoing:≈ Continuing to collect phenotype data from the
community.≈ Starting to annotate early papers describing large
collections of mutants -> many high-level phenotype annotations.
≈ Starting to annotate new papers.
Currently, 4,401 total allele-phenotype connections to 2585 alleles, defining 1296 genes.
PATO, December 2006
Lots of RNAi data -> dense Lots of RNAi data -> dense early_embryonic_lethal node:early_embryonic_lethal node:
PATO, December 2006
Vague collections of phenotypes Vague collections of phenotypes present challenges for present challenges for ontology/annotation:ontology/annotation:
pleiotropic_defects_severe_early_emb:“Often multiple pronuclei, aberrant cytoplasmic texture, drop in overall pace of development, osmotic sensitivity.”
complex_phenotype_early_emb“Complex combination of defects that does not match other class definitions.”
PATO, December 2006
Looking ahead to an entity-quality Looking ahead to an entity-quality compatible schema:compatible schema:
≈ Within OBO-Edit we store relevant GO term names within primary names or synonym names (GO ID stored in relevant dbxref field)
≈ Phenotype ontology is developed using existing anatomy and life stage term names
PATO, December 2006
Phenotype data integration:Phenotype data integration:≈ Phenotype annotations are associated with
molecular information for alleles, transgenes, and RNAi objects that permit mapping these objects to the genome.
≈ High-level phenotype annotations associated with RNAi objects are automatically converted to GO terms (RNAi2GO) and associated with gene objects.
≈ Phenotype annotations describing gene regulation (‘transgene_expression_abnormal’) linked with detailed gene regulation information.
≈ Phenotypes linked to life stage and anatomy term
PATO, December 2006
RNAi summary on gene page:RNAi summary on gene page:
PATO, December 2006
Sample detailed RNAi report:Sample detailed RNAi report:
PATO, December 2006
Sample allele report:Sample allele report:
PATO, December 2006
Immediate future plans:Immediate future plans:
≈ Ontology:≈ Define terms, further refine ontology
(expansion will be dictated by community feedback and curation needs)
≈ Solicit more expert community feed-back
≈ Web site:≈ Enhance phenotype search tools
PATO, December 2006
Ontology browser to be integrated Ontology browser to be integrated into WormBase:into WormBase:
http://elbrus.caltech.edu/cgi-bin/igor/ontology/ontology.cgi
PATO, December 2006
WormBase = ~30 people, 4 centersWormBase = ~30 people, 4 centersCold Spring Harbor Laboratory
Payan CanaranJack ChenTristan FiedlerTodd HarrisSheldon McKayWill SpoonerLincoln Stein
California Institute of Technology
Igor AntoshechkinCarol BastianiJuancarlos ChanWen ChenRanjana KishoreRaymond LeeHans-Michael MüllerCecilia NakamuraAndrei PetcherskiGary SchindelmanErich SchwarzPaul SternbergKimberly Van AukenDaniel WangXiaodong Wang
Washington University at St. Louis
Tamberlyn BieriDarin BlasiarPhil OzerskyJohn Spieth
Wellcome Trust Sanger Institute
Paul DavisRichard DurbinMichael HanAnthony RogersMary Ann TuliGary Williams
PATO, December 2006
≈ NIH/NHGRI≈ C. elegans research community
Other acknowledgements:Other acknowledgements: