NanoBrook 173Protein Sizer

Polymer SizeParticle Size of Proteins

NanoBrook 173Protein Sizer

Brookhaven’s NanoBrook 173 instrument is the go-to-solution for characterization of proteins, antibodies, nanoparticles, macromolecular assemblies, and other molecules or particles of small sizes. The NanoBrook 173 uses backscattering detection for the highest sensitivity and accuracy optimized for molecules and particles less than a few tens of nanometers.

Reliable and Accurate Analysis

The new NanoBrook 173 protein and particle size analyzer incorporates all you need for fast, routine, sub-micron measurements. Based on the principles of Dynamic Light Scattering (DLS) for particle sizing and distribution, most measurements only take a minute or two.

Nanoparticles

Dilute suspensions, on the order of 0.0001 to 1.0% v/v, are prepared using suitable wetting and/or dispersing agents, if required. A small ultrasonicator is sometimes useful in breaking up loosely-held agglomerates. Sample volumes as small as 50 µL are used. Sample is recoverable. Disposable, polystyrene U-shaped cells are used for aqueous and simple alcohol suspensions. Just a few minutes are required for the sample and cell to equilibrate with the actively controlled temperature environment inside the NanoBrook 173.

• Rapid and accurate protein & nanoparticle size distributions• Protein molecular weight• Polymer size & molecular weight• Multimodal & unimodal size distribution software• ISO 13321 and ISO 22412 compliant results• Range: 0.3 nm to 10 µm• Customizable reports• Ideal for fast, routine sizing applications in research or quality

control• High power 40 mW temperature-controlled semiconductor laser• Avalanche Photodiode detector with highest Quantum Efficiency

and low dead time• Dynamic light scattering at 173°• Temperature control, -5 °C to 110 °C• Compact bench top unit, USB connection

Speed and low concentration/small size capabilities

The NanoBrook 173 utilizes backscattering to size the proteins/particles by accumulating the correlation function. The smoother the function, the better the results are. For small molecules, the scattered intensity is typically low. The measurement takes longer to aquire enough statistics from the photons counted to obtain a good results. The backscattering angle makes for a larger scattering volume, measuring more proteins/particles for a quick measurement of their sizes. The advantage of the backscattering is clear for sizes below 10 nm.

Data Presentation

The NanoBrook 173 protein and particle size analyzer offers many choices for data analysis. One choice is the lognormal distribution by: intensity, volume, surface and number. Another choice, the full size distribution (MSD) can be displayed by the same four weighting factors. In the example above, the MSD is presented by volume for lysozyme. The size distribution and cumulative distribution are shown in the table on the right. Lysozyme has a very low polydispersity in this case.

The new NanoBrook 173Plus particle size analyzer incorporates all you need for fast, routine, sub-micron measurements. Based on the principles of Dynamic Light Scattering (DLS) for particle sizing and distribution, most measurements only take a minute or two. The bimodal analysis to the rigt demonstrates the power of the 173Plus.

Accurate and Reliable

NanoBrook 173Protein Sizer

Specifications

Sample Type Most globular proteins, nano particles, and small polymers in any non-absorbing liquid

Sample Cells 50 µL disposable, 1 to 3 mL disposable plastic, glass, or quartz cells

Concentration Range 0.1 ppm to 50 mg/mL, depending on refractive index and concentration

Signal Processing Dynamic Light Scattering, DLS

Correlator Brookhaven’s TurboCorr, multitau, research grade with 522 channels, covering the equivalent of 1010 linearly-spaced channels, 100% efficiency, real-time operation over the entire delay-time range.

Precision Sizing: ± 1% typical

Temperature Control -5 °C to 110 °C, ± 0.1 °C, active control. No external circulator required.

Condensation Control Purge facility using dry air, nitrogen preferred

Standard Laser

Scattering Angle 173°

Data Presentation Average & width, lognormal fit, and multimodal size distribution standard

Compliance ISO13321 and ISO22412 compliant results for sizing

Power Requirements 100/115/220/240 VAC, 50/60 Hz, 150 Watts

Dimensions 23.3 x 42.7 x 48.1 cm (HWD)

Weight 15 kg

Environmental Characteristics Temperature 10 °C to 75 °C Humidity 0% to 95%, non-condensing

CE Certificate Class I laser product, EN 60825-1:2001, CDRH

750 Blue Point Road Holtsville, NY 11742-1832 USA

info@brookhaveninstruments.com www.BrookhavenInstruments.com Telephone: +1 631.758.3200Fax: +1 631.758.3255

Size Range

40 mW 640 nm temperature-controlled red semiconductor laser. Alternative wavelengths available.

Sizing: 0.3 nm to 10 µm diameter, depending on refractive index and concentration Molecular Weight: ~980 Da to 20 MDa, sample dependent