Upload
nidia-maradiaga
View
217
Download
0
Embed Size (px)
Citation preview
8/3/2019 Worksafe Blood Culture Collection Procedure 1broch
http://slidepdf.com/reader/full/worksafe-blood-culture-collection-procedure-1broch 1/1
1. Skin PreparationPDI® Chlorascrub™ Swabstick
• Afterlocationofthevein,vigorouslyscrubthevenipuncture sitewithPDI®Chlorascrub™Swabstickfor30seconds.1
• Allowthesitetoairdrybeforevenipuncture.
•Do not re-palpate the vein.
2.BottlePreparation • Inspecteachbloodculturebottlebeforeusetoensureintegrity
ofbottleandsensoronbottomofbottleisintact.Thesensor isnormallyauniformgrayish-greencolorandayellowcolor wouldindicatecontaminationofthebroth.Discardanybottle foundtobedamagedorwithasensorthatisyellow.
• Removeprotectiveiptopovercap.
•NOTE:Theseptumisnotsterileandmustbedisinfected.
• Cleansetheseptumwith70%alcohol.
•Thebottlehasbeenpre-markedwith5mlincrements. Markthedesiredllvolumelevelonthebottlefor10ml.
3.VenipunctureandBottleInoculation Direct Draw with Blood Collection Set or WorkSafe™ Blood Culture Collection Kit
• Collectallnecessarymaterialsforadirectdraw:
from diagnosis,the seeds of better health
BacT/ALERT®
Blood Culture Collection Procedure
Availability WorkSafeBloodCultureCollectionKit 279102-279105*
Saf-THolder®BloodCultureDevice(AdaptorCapandInsert) 100/case 96004**
*seebioMérieuxcatalogue
**seeSmithsMedicalcatalogue
bioMérieux, Inc.100RodolpheStreetDurham,NC27712U.S.A.Tel: (800)682-2666Fax: (800)968-9494
bioMérieux Canada, Inc.7815Henri-BourassaBoulevardOuestSt.Laurent(Québec)Canada H4S 1P7Tel:(514)336-7321Fax:(514)807-0015
1-800-361-7321ext.221 www.biomérieux-usa.com
© 2
0 0 8 B I O M É R I E U X ,
I N C .
B A C T / A L E R T ® A
N D
B A C T / A L E R T ® 3
D A R E R E G I S T E R E D T R A D E M A R K S O F B I O M É R I E U X .
S A F - T
H O L D E R ® I S
A R E G I S T E R E D T R A D E M A R K O F T H E S M I T H S M E D I C A L F A M I L y O F C O M p A N I E S .
C H L O R A S C R U B ™ I S A T R A D E M A R K O F p D I . p R I N T E D I N
U S A .
B T A - 1 2 5 - 0 8 .
Tips ’n’ Hints•Recommendedbloodtobrothratiois1:5to1:10.Asthevolumeofblooddrawnisincreased,theyieldofpositiveculturesincreases.Optimally,20mlofbloodshouldbedrawnfromadults(10mlperbottle).
•Whenlabelingthebottles,donotcoverthe peel-offsectionofthebarcodelabelsorthe lotnumbers.
•Orientadditionalpatientlabelverticallyfor scanningefciency.
•Forbestvolumecontrol,marklllevelonsideofbottlepriortocollection.
•Donotoverllthebottles,asthismaycause falsepositivereadings.
•WhenusingtheSaf-THolder®BloodCultureDevice(AdaptorCapandInsert),besureluerisconnectedrmlyandtheneedleisstraightwhenenteringandleavingtheseptum.Twistingorturningthebottlemayincreasethechancethatthesheathmaynotretractandreseal.
•Toavoidcontaminationofthebloodculturesample,inoculatebloodculturebottlesrst. Thenlladditionalbloodcollectiontubes.
Septicemiaoccursinapproximately200,000patientseachyear,andbetween30and50%ofthosepatientsdie.Becausethe consequencesofsepticemiaaresoserious,rapidandaccuratediagnosisofthisdisorderiscritical.
TheBloodCultureisthebestdiagnostictoolfordetectingsepticemia,andit’sabsolutelyimperativethatthebloodspecimenbecollectedproperlytoprovideclinicallyvaluableresults. BacT/ALERT®3Dinstrumentsareautomatedsystemsthatincubate,shakeandmonitorculturebottlesforsignsofmicrobialgrowth. SamplesfrompatientssuspectedforsepticemiaareinoculatedintoBacT/ALERTculturebottlesformonitoring.Thebottlesarecolor codedforeasyidenticationofmediatypeandaremadeofplasticforaddedsafety.
Performvenipuncture. Whentheneedleisinthevein,secureitwith
tapeorholditinplace.
2.
PlaceAdaptercapontheaerobic(blueorgreenlabel)BacT/ALERTculturebottle
septumandpressdowntopenetrateandobtainbloodow.HoldtheAdapterCapdownon thebottle.
3.
E CI E G
F RZ E REFRIGERTE
R T E P ER T U RE
I H
Usingthellindicatorlinesonthelabel,obtain8-10mlofblood.Move
theAdapterCapfromtheaerobicbottletotheanaerobic(purpleororangelabel)bottle(ifrequired)andcontinuethecollection.
4.
E CI E G
F RZ E RE F RI GE RTE
R T E P ER T U RE
I H
Ifadditionalbloodisrequiredforothertests,placetheAdapterInsert
intotheAdapterCapandsnapintoplace.Thismakesthecapcompatiblewithvacuumcollectiontubes.
5.
Whennaltubeislled,removetourniquet.Terminatethe
venipuncture,activatethesafetyshield,grabthewingwithonehandandslidethewingsbackuntilitlocksinplace.
6. E CI E G
F RZ E RE F RIGE RTE
R T E P ER T U RE
I H
Disposeofwing andadaptercapin asharpscontainer.
7. SPECIMENBAG
F RO ZE N RE F RIGE RA TE
ROOM TEMPERATURE
BIOHAZARD
Labelspecimensandplaceinplasticbiohazardziplockbag.Bottlescannow
besafelytransportedviapneumatictubesystem.
8.
OR
GatherTourniquet,BacT/ALERT®bottles, Saf-THolder®Adapter,wing,Chlorascrub™Swabstick,gauze,alcoholpreppads.
1perbioMérieux/Cumitechguidelinesasthemanufacturercannotmakethispreptimerecommendation
1a.
OpentheWorkSafeBloodCultureCollectionKitwhichcontainsallthenecessarymaterialsinasingle,convenientpackage.
1b.