Wheaton Scientific Products

P R O V E N T O O L S™ F O R R E S E A R C H

2 0 0 8 - 0 9 P R O D U C T C A T A L O G

2008-09 CATALOG

9833 WHT front cov.:Layout 1 1/16/08 9:13 AM Page 1

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WHEATON 1.800.225.1437 (U.S. & Canada only) | +1.856.825.1100 | +1.856.825.1368 (F) | www.wheatonsci.com

For biological sample preservationAllows for storage at low temperaturesCan be used in the gas phase of liquid nitrogen

These glass cryogenic vials are designed for preserving biological materials with liquid nitrogen. They are made of Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM Type I, Class A requirements. The special design allows for storage at low temperatures as well as rapid thawing. Cat. No. Size (mL) Dia x H (mm) *OD at Top (mm) Qty/Case

651462 1 10.5 x 61 5 144651463 1.2 11.8 x 58 5 144651464 1.5 11.5 x 61 5 144651466 2 11.5 x 70 5 144651469 5 16.5 x 98 8 144651470 10 19.5 x 114 9 144

* Approximate OD

Pre-scored to eliminate filingFor biological sample preservationAllows for storage at low temperaturesCan be used in the gas phase of liquid nitrogen

These glass cryogenic vials are designed for preserving biological materials with liquid nitrogen. They are made of Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM Type I, Class A requirements. Their special design allows for storage at low temperatures as well as rapid thawing. Pre-scored ampules eliminate the need for filing. Cat. No. Size (mL) Dia x H (mm) *OD at Top (mm) Qty/Case

651482 1 10.5 x 61 5 144651483 1.2 11.8 x 58 5 144651486 2 11.5 x 70 5 144

* Approximate OD

Ideal for small volume lyophilization samplesHeat or stopper sealed

This small volume lyophilization ampule can be flame-sealed or stored with a stopper in place. They are made of Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM Type I, Class A requirements. Stoppers can be purchased separately.Cat. No. Description Dia x H (mm) *OD at Top (mm) Qty/Case

651446 2 mL Vacule 12 x 86 10 144W651450 11 mm Stopper — — 144

* Approximate OD

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Wheaton ampules are all glass containers that can be hermetically sealed to preserve sample purity and extend sample shelf life. Wheaton ampules are ideal for standards packaging, environmental and diagnostic standards, lyophilization and cryogenic storage. These ampules protect against sample contamination due to their tamper-evident seal. A gold band on the stem of the ampule indicates that the ampule is pre-scored to facilitate snapping off the top of the ampule eliminating the need to file the ampule.

Wheaton ampules are offered in sizes ranging from 1 to 50 mL capacity. Our line includes pre-scored, funnel-top, standard, glass Cryule® cryogenic ampules and Vacule® lyophilization ampules. Wheaton can also design a custom ampule to fit your special requirements.

Standard Ampules

Tip Seal Method: While holding the ampule body, insert the tip of the ampule stem into the sealing flame. Heat the tip approximately 3-4 mm from the top. Gently rotate the ampule while heating the tip until the melted glass fuses into a smooth dome.

Funnel-Top Ampules

Pull Seal Method: In addition to easier filling, the funnel-top ampule is easier to seal using the Pull Seal method. Hold the funnel top with one hand and the body of the ampule with the other hand. Insert the center of the ampule stem into the sealing flame. As the glass softens, rotate the ampule while pulling the funnel top off. This will require very little force and will allow the glass to seal itself. This method can be used for all ampule configurations.

651462 Series 651482 Series Pre-Scored

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Ideal for freeze dryingPre-scored to eliminate filingHeat sealed only

These ampules are designed for freeze drying, protein hydrolysis, and micro-determination of ketones and amino acids. The long stem provides a conve-nient means for heat sealing under vacuum. The pre-scored feature allows for easy opening. They are made of Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM Type I, Class A requirements.Cat. No. Size (mL) Dia x H (mm) *OD at Top (mm) Qty/Case

651502 1 10 x 108 10 144651506 2 11.8 x 122 11.75 144651509 5 16.5 x 133 9 144651510 10 19.5 x 154 10 144

* Approximate OD

Pre-scored to eliminate filingAvailable in clear or amber glassStems can be pull or tip sealed

The stem can be pull sealed or tip sealed to seal the ampule (See sealing methods on previous page). The pre-scored feature saves time by eliminat-ing filing. The clear glass ampules are made of Wheaton 33 expansion low extractable borosilicate glass that conforms to USP Type I and also ASTM Type I, Class A requirements. The amber ampules are manufactured from Wheaton 320 amber borosilicate glass that conforms to USP Type I requirements and protects light-sensitive samples.Cat. No. Size (mL) Dia x H (mm) Qty/Case

Clear 176772 1 10.5 x 67 144176776 2 12 x 75 144176779 5 16.5 x 84 144176780 10 19 x 107 144176782 20 22.5 x 130 144176784 50 29.5 x 175 144

Amber176792 1 10.5 x 67 144176796 2 12 x 75 144176799 5 16.5 x 84 144

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Funnel top provides easier fillingStem is pull sealedAvailable in clear glass only

Pre-scored ampules eliminate the need for filing. Ampules are made from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and also ASTM Type I, Class A to prevent change of pH and to maintain the purity of contents. Cat. No. Size (mL) Dia x H (mm) Qty/Case

176732 1 10.5 x 88 144176740 10 19 x 130 144

Commonly used for packaging certified standardsConsistent with low background counts

These ampules are utilized for sealed standards used in calibrating liquid scintillation spectrometers. Manufactured from Wheaton 180 low potassium borosilicate glass that meets USP Type I and ASTM Type I, Class A requirements for consistent and low background counts. Cat. No. Size (mL) Dia x H (mm) Qty/Case

176759 7 17 x 100 25176762 20 28 x 100 25

Protects against glass splintersPrevents cross contamination

This disposable plastic fracture and safety collar protects against glass splinters when used to break open pre-scored ampules. Just slip the Ampule Snapper over the top of the ampule, slide it down to the pre-scored position, and snap it. The Ampule Snapper and ampule top can be discarded to prevent cross-contamination.

Cat. No. For Ampule Size Qty/Case

177105 1 & 2 mL 144

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Wheaton BOD Bottles are ideal for incubating diluted samples of sewage, sewage effluents, polluted waters, and industrial wastes to determine the amount of oxygen required during the stabilization of the decomposable organic matter by aerobic biochemical action. We recommend the 300 mL capacity bottles for the five-day BOD test as referenced in Standard Methods for the Examination of Water and Wastewater, 21st Edition, 5210B, 2005.

These BOD bottles have a specially designed shoulder radius that sweeps all air from inside the bottle during filling. The interchangeable stoppers have a tapered bottom that also prevents air entrapment. The bottles have a flared mouth to form a water seal around the stopper that prevents air from being drawn into the bottle during incubation. The stopper joint is compatible with the probes of the leading meters for BOD and dissolved oxygen.

All of the BOD bottles have large, permanently screened-on marking areas. The numbered versions of the 300 mL size bottles are supplied with bar codes for quick and accurate recording of bottle numbers. All of the bottles are manufactured from Type I borosilicate glass.

For 60 mL and 300 mL BOD Bottles

Cat. No. Description Qty/Case

227670 Robotic Stopper, Glass 12227672 Pennyhead Stopper, Glass 12

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Cat. No. Number Sequence Dia x H (mm) Qty/Case

With Glass Pennyhead Stopper227494-00G Un-numbered 43 x 115 36227494-01G 01-36 43 x 115 36227494-02G 37-72 43 x 115 36227494-03G 73-108 43 x 115 36227494-99G Specials 43 x 115 36

With Glass Robotic Stopper227494-00 Un-numbered 43 x 115 36227494-01 01-36 43 x 115 36227494-02 37-72 43 x 115 36227494-03 73-108 43 x 115 36227494-99* Specials 43 x 115 36

Cat. No. Number Sequence Dia x H (mm) Qty/Case

With Glass Pennyhead Stopper 227497-00G Un-numbered 69 x 165 24227497-01G 01-24 69 x 165 24227497-02G 25-48 69 x 165 24227497-03G 49-72 69 x 165 24227497-04G 73-96 69 x 165 24227497-05G 97-120 69 x 165 24227497-06G 121-144 69 x 165 24227497-07G 145-168 69 x 165 24227497-08G 169-192 69 x 165 24227497-09G 193-216 69 x 165 24227497-10G 217-240 69 x 165 24227497-11G 241-264 69 x 165 24227497-12G 265-288 69 x 165 24227497-13G 289-312 69 x 165 24227497-14G 313-336 69 x 165 24227497-15G 337-360 69 x 165 24227497-16G 361-384 69 x 165 24227497-17G 385-408 69 x 165 24227497-18G 409-432 69 x 165 24227497-99G* Specials 69 x 165 24

With Glass Robotic Stopper227497-00 Un-numbered 69 x 165 24227497-01 01-24 69 x 165 24227497-02 25-48 69 x 165 24227497-03 49-72 69 x 165 24227497-04 73-96 69 x 165 24227497-05 97-120 69 x 165 24227497-06 121-144 69 x 165 24227497-07 145-168 69 x 165 24227497-08 169-192 69 x 165 24227497-09 193-216 69 x 165 24227497-10 217-240 69 x 165 24227497-11 241-264 69 x 165 24227497-12 265-288 69 x 165 24227497-13 289-312 69 x 165 24227497-14 313-336 69 x 165 24227497-15 337-360 69 x 165 24227497-16 361-384 69 x 165 24227497-17 385-408 69 x 165 24227497-18 409-432 69 x 165 24227497-99* Specials 69 x 165 24

Bottle Without Stopper227498 Un-numbered 69 x 143 24

*For specials contact Wheaton Customer Service.

60 mL BOD Bottle 300 mL BOD bottle

Pennyhead Stopper Robotic Stopper

For environmental testing apparatus see pages 65-82. For sampling devices see pages 129-132.

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Polyethylene cap prevents evaporationIncludes foam insert

This polyethylene cap, complete with a closed cell foam insert, snaps firmly over the flared mouth of Wheaton BOD bottles. This closure prevents the evaporation of the water seal during the five-day BOD incubation period. The foam insert fits into the cap and exerts pressure on the bottle stopper to hold it securely in place.


227723 BOD Bottle Cap 50

Conveniently transports bottles safelyRacks are stackable

These stackable, rugged wire racks are plastic-coated for corrosion resistance and quietness. They provide a convenient way to transport large amounts of bottles in the lab or in the field. The rack comes in three different sizes, which hold the 60 mL or 300 mL bottles.

Cat. No. For Bottle Size Holds L x W x H (in/cm) Qty/Case

227728 60 mL 20 Bottles 13 x 10 x 7 in (33.03 x 25.4 x 17.78 cm) 1227730 300 mL 12 Bottles 13 x 10 x 7 in (33.03 x 25.4 x 17.78 cm) 1227735 300 mL 24 Bottles 19 x 12 x 7 in (48.26 x 30.48 x 17.78 cm) 1

Never lose a stopper againKeeps stopper secure to the neck of bottle

This rubber leash ensures that the stoppers do not become lost when collecting samples in from the field. The 10 mm diameter holes at each end of the leash expand to fit securely around both the stoppers and the bottle necks.


776580 Stopper Leash 25

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For light sensitive samplesWith glass Robotic stopper

This is a standard 300 mL BOD bottle with black PVC plastic coating that blocks all visible light up to 800 nm. This inhibits the production of oxygen by algae. We recommend this bottle for use in marine photosynthesis projects in which you need to compare oxygen in a light and a dark bottle. This bottle is supplied complete with a glass robotic stopper and an opaque black cap. To replace stopper use catalog number 227670.Cat. No. Dia x H (mm) Qty/Case

227667 73 x 167 20

With glass Robotic stopperLarge capacity

We recommend this 2 Liter BOD Bottle for long-term BOD and ultimate BOD analysis. This bottle is manufactured from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and also ASTM E-438 Type I, Class A requirements and comes complete with a glass robotic stopper. To replace stopper use catalog number 227670.Cat. No. Dia x H (mm) Qty/Case

227580 128 x 272 1

40-60 micron porosity glass fritLow extractable borosilicate glassFor use with 300 mL bottleEPA Method 245.1 Manual Cold Vapor

Technique

This unit is useful in determining inorganic forms of mercury and organic mercurials in drinking, surface, and saline waters, as well as domestic and industrial waste. The Aerator has a 40-60 micron porosity cylindrical glass frit that provides even bubble distribution. It is manufactured from Wheaton 200 low extractable borosilicate glass that conforms to USP Type I and also ASTM E-438 Type I, Class A requirements. Reference: U.S. EPA Method 245.1, “Manual Cold Vapor Technique.”


227700 BOD Aerator 1

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Used to store and dispense liquidsPerfect for educational use30 mL capacityAverage drop size is 46 μLGlass pipette holds approximately 480 μL

This clear, square shaped bottle is manufactured from Wheaton 400 borosilicate glass that conforms to USP Type I requirements. The lower portion of the isoprene rubber bulb forms a tightly fitting stopper. The bottle is supplied with the bulb and pipette. Replacement bulbs and pipettes are available separately.

Cat. No. Description Dia x H (mm) Qty/Case

W211523 30 mL capacity 35 x 103 6211523 30 mL capacity 35 x 103 36211530 Replacement bulbs — 100211533 Replacement bulbs & pipettes — 100

Delivers stains or indicator solutions drop by dropAvailable in clear or amber glassGround glass stopper50 and 100 mL capacity

These bottles deliver stains and indicator solutions drop by drop from a projecting drip-tip on the side of the stopper. A partial turn of the ground glass stopper seals the bottle. Amber bottles are perfect for storing light-sensitive materials. Bottles are manufactured from soda-lime glass that conforms USP Type III requirements. Not autoclavable.

Cat. No. Capacity (mL) Dia x H (mm) Qty/Case

ClearW211734 50 45 x 95 6W211735 100 56 x 120 6

AmberW211754 50 45 x 95 6W211755 100 56 x 120 6

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W211734, W211755

Use these bottles to determine if any of 40 contaminants are in waste mate-rial. Heavy-duty borosilicate bottles are used in EPA methods 1310 Extraction Procedure, 1311 Toxicity Characteristic Leaching Procedure (TCLP,) and 1312 Synthetic Precipitation Leaching Procedure (SPLP) for those substances subject to regulation under Subtitle C of the Resource Conservation and Recovery Act (RCRA). The EPA recommends that borosilicate bottles be used. All TCLP bottles have GPI 100-400 thread finish. Pre-cleaned TCLP bottles are available on special order. Contact Wheaton Science Products for more information.

Cat. No. Capacity (L) Description OD x Length (mm) Qty/Case

216711 2.2 Single-End Opening 110 x 325 1216712 2.2 Double-End Opening 110 x 334 1216731 3.2 Single-End Opening 110 x 450 1

100-400 mm polypropylene screw capFor use with TCLP Extraction Bottles Available in 3 stylesPurchase liners separately


240202 White heavy duty PP screw cap; resilient to mechanical and chemical attack 4240203 White heavy duty PP screw cap with center hole. Use with Zitex® Cap Liner (Cat. No. 240555) to self-vent the bottle. 4240204 White light duty PP screw cap with a shorter skirt 4

For TCLP Extraction BottlesZitex® liner provides unique

venting propertiesPTFE / Silicone liner provides

excellent chemical resistance

The Zitex® liner consist of a laminate that combines Zitex®, a porous form of PTFE with unique venting properties, to a resilient foam backing. Zitex® also provides chemical resistance and a hydrophobic barrier that prevents pressure buildup in a container. The PTFE/Silicone liner consists of 0.005" PTFE bonded to 0.060" silicone and provides excellent chemical resistance.

Cat. No. Description For Cap Size (mm) Qty/Case

240555 Zitex® Cap Liner 100 - 400 4240557 PTFE/Silicone Cap Liner 100 - 400 4

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240202 240203 240204

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HAZCOM label allows for ID of content and safety notificationLabels provided in each case of bottles with the exception of bulk packsTamper Evident Seal keeps bottle sealed, protecting contents from

accidental misuse

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Bottles manufactured from soda-lime glass that conforms to USP Type III requirements

Available in clear glass or amber glass for light sensitive samplesNarrow mouth bottles are ideal for liquidsWide mouth bottles are ideal for dry and viscous samplesValumetric bottle are for measuring while you sample White polypropylene or black phenolic screw capsScrew caps are pre-attached to the containers or are available separatelyChoice of cap liners: Teflon® faced foamed polyethylene liner, Poly-Vinyl

liner, Rubber liner, Teflon® faced rubber, or Poly-Seal® (cone) linerTamper Evident Seal/HAZCOM Label provided in each case of bottles with

the exception of bulk packs

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Packaged in convenience packs with caps attached

Convenience Bulk Packs provide larger quantities with or without caps attached for high use items or facilities with centralized stockrooms

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Actual Label Size

Material Description ApplicationsPoly-Vinyl General purpose: Suitable for wide range of applications. Chemical

resistance: Good for mild acids, alkalis, solvents, alcohols, oils and aqueous products. Poor for active hydrocarbons and bleaches.

One mil poly-vinyl film bonded to one mil HDPE on a #30 white pulp paper backing. Superior to plain pulp paper because it provides an excellent moisture barrier.

Typical applications: analytical lab samples, high purity chemicals, strong acids, solvents. Excellent for environmental samples, pharmaceuticals and diagnostic reagents.

Teflon faced foamed polyethylene liner that offers the excellent chemical resistance of Teflon with the compressibility and sealing properties of polyethylene foam.

Teflon® Faced Foamed Polyethylene

Unique problem solving type of liner. This liner is stress crack resistant and offers superior torque retention and excellent sealing characteris-tics. It is recommended that this liner be tested prior to use for leak seal.

Manufactured from polyethylene (LDPE). The unique cone design provides a wedge type seal that not only seals across the top but also across the inside diameter.

Poly-Seal®

Excellent properties of resilience, resistant to moisture vapor. Satisfactory for most moderate chemicals. Not good for oils, strong acids and hydrocarbons. Autoclavable.

The 14B white rubber lining material consists of homogeneous sulfur cured styrene-butadiene rubber. FDA Status complies with 21CFR 177.26, “Rubber articles intended for repeated use.”

Styrene-Butadiene Rubber (14B)

Designed for the ultimate in product safety. PTFE provides totally inert inner seal and surface facing the sample or product. Autoclavable.

The white rubber/0.005" PTFE liner consists of virign PTFE bonded to the white sulfur cured styrene-butadiene rubber. Complies with the FDA 21CFR 177.1550.

Teflon® Faced Rubber

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30-1000 mL (1 oz-32 oz) sizesPolypropylene caps with choice of linersClear or Amber glass for light sensitive samples

Narrow Mouth Bottles are available in both clear and amber Type III soda-lime glass and conform to USP Type III requirements. These bottles are ideal for solvent, chemical and sample storage and are packaged in convenience packs with caps attached or bulk packs with or without caps. Caps may also be purchased separately.

Cat. No. Capacity (oz) Capacity (mL)* Dia x H (mm) Cap Size Qty/Case

With White Polypropylene Cap/Poly-Vinyl LinerW216800 1 30 31 x 79 20-400 48W216801 2 60 39 x 94 20-400 24W216802 4 125 48 x 112 22-400 24W216803 8 250 60 x 136 24-400 12W216804 16 500 75 x 168 28-400 12W216805 32 1000 94 x 206 33-400 12

With White Polypropylene Cap/Teflon® Faced Foamed Polyethylene LinerW216806 1 30 31 x 79 20-400 48W216807 2 60 39 x 94 20-400 24W216808 4 125 48 x 112 22-400 24W216809 8 250 60 x 136 24-400 12W216810 16 500 75 x 168 28-400 12W216811 32 1000 94 x 206 33-400 12

With Black Phenolic Cap/Poly-Seal® LinerW216812 1 30 31 x 79 20-400 48W216813 2 60 39 x 94 20-400 24W216814 4 125 48 x 112 22-400 24W216815 8 250 60 x 136 24-400 12W216816 16 500 75 x 168 28-400 12W216817 32 1000 94 x 206 33-400 12

With Black Phenolic Cap/Rubber LinerW216818 1 30 31 x 79 20-400 48W216819 2 60 39 x 94 20-400 24W216820 4 125 48 x 112 22-400 24W216821 8 250 60 x 136 24-400 12W216822 16 500 75 x 168 28-400 12W216823 32 1000 94 x 206 33-400 12


Bottles OnlyW216830 1 30 31 x 79 20-400 432W216831 2 60 39 x 94 20-400 288W216832 4 125 48 x 112 22-400 160W216833 8 250 60 x 136 24-400 108W216834 16 500 75 x 168 28-400 60W216835 32 1000 94 x 206 33-400 12

*Approximate capacity

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Clear USP Type III soda-lime glass30-500 mL (1 oz-16 oz) sizes

AC Round Bottles have a taller and narrower profile than Straight Sided Jars. These bottles come packaged in convenience packs with caps attached or bulk packs without caps. Caps may also be purchased separately.


With White Polypropylene Cap/Poly-Vinyl LinerW216995 1 30 34 x 68 33-400 48W216996 2 60 42 x 83 38-400 48W216997 4 125 51 x 102 48-400 24W216998 8 250 62 x 127 58-400 24W216999 16 500 76 x 145 70-400 24

With White Polypropylene Cap/Teflon® Faced Foamed Polyethylene LinerW217000 1 30 34 x 68 33-400 48W217001 2 60 42 x 83 38-400 48W217002 4 125 51 x 102 48-400 24W217003 8 250 62 x 127 58-400 24W217004 16 500 76 x 145 70-400 24

Bottles OnlyW217005 1 30 34 x 68 33-400 432W217006 2 60 42 x 83 38-400 288W217007 4 125 51 x 102 48-400 144W217008 8 250 62 x 127 58-400 96W217009 16 500 76 x 145 70-400 48

*Approximate capacity.

Cat. No. Cap Size Qty/Pack Qty/Case

White Polypropylene/Teflon® Faced Foamed Polyethylene Liner239236 33-400 72 144239237 38-400 72 72239240 48-400 72 72239242 58-400 72 72239244 70-400 24 48

White Polypropylene/Poly-Vinyl Liner239212 33-400 72 144239213 38-400 72 72239216 48-400 72 72239218 58-400 72 72239220 70-400 24 48

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Tamper Evident Seal / HAZCOM Label Labels provided in each case of bottles with the exception of bulk packs

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15-500 mL (0.5 oz-16 oz) sizesSquare shape maximizes storage spaceClear for sample observation4 different cap liners

French Square Bottles are made with USP Type III soda-lime glass and conform to USP Type III requirements. The square design allows for full utilization of any storage area, maximizing space efficiency. Bottles are packaged in convenience packs with caps attached or bulk packs with or without caps. Caps may also be purchased separately.


With White Polypropylene Cap/Poly-Vinyl LinerW216871 .5 15 26 x 62 20-400 48W216872 1 30 31 x 72 24-400 48W216873 2 60 39 x 87 28-400 48W216874 4 125 45 x 111 33-400 24W216875 8 250 56 x 137 43-400 24W216876 16 500 68 x 167 48-400 24

With White Polypropylene Cap/Teflon® Faced Foamed Polyethylene LinerW216877 .5 15 26 x 62 20-400 48W216878 1 30 31 x 72 24-400 48W216879 2 60 39 x 87 28-400 48W216880 4 125 45 x 111 33-400 24W216881 8 250 56 x 137 43-400 24W216882 16 500 68 x 167 48-400 24

With Black Phenolic Cap/Rubber LinerW216883 .5 15 26 x 62 20-400 48W216884 1 30 31 x 72 24-400 48W216885 2 60 39 x 87 28-400 48W216886 4 125 45 x 111 33-400 24W216887 8 250 56 x 137 43-400 24W216888 16 500 68 x 167 48-400 24

With Black Phenolic Cap/Poly-Seal® LinerW216889 .5 15 26 x 62 20-400 48W216890 1 30 31 x 72 24-400 48W216891 2 60 39 x 87 28-400 48W216892 4 125 45 x 111 33-400 24


Bottles Only W216898 1 30 31 x 72 24-400 280W216899 2 60 39 x 87 28-400 240W216900 4 125 45 x 111 33-400 120W216901 8 250 56 x 137 43-400 84W216902 16 500 68 x 167 48-400 40


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With Black Phenolic Cap/Poly-Seal® LinerW216848 1 30 31 x 79 20-400 48W216849 2 60 39 x 94 20-400 24W216850 4 125 48 x 112 22-400 24W216851 8 250 60 x 136 24-400 12W216852 16 500 75 x 168 28-400 12W216853 32 1000 94 x 206 33-400 12

With Black Phenolic Cap/Rubber LinerW216854 2 60 39 x 94 20-400 24W216855 4 125 48 x 112 22-400 24W216856 8 250 60 x 136 24-400 12W216857 16 500 75 x 168 28-400 12W216858 32 1000 94 x 206 33-400 12





Black Phenolic with Rubber LinerW239298 20-400 72 144W239299 22-400 72 144W239300 24-400 72 144W239301 28-400 72 144W239302 33-400 72 144

Black Phenolic/PE Poly-Seal® Liner239253 20-400 72 144239255 22-400 72 144239257 24-400 72 144239259 28-400 72 144239260 33-400 72 144



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Clear USP Type III soda-lime glass60-1000 mL (2 oz-32 oz) sizesWide mouth design is great for large solid samplesWalls allow for complete removal of contentsIdeal for soil sampling and environmental applications

Straight Sided Jars are packaged in convenience packs with caps attached or bulk packs with or without caps. Caps may also be purchased separately.




With Black Phenolic Cap/Rubber LinerW216913 4 125 60 x 68 58-400 24

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Black Phenolic with Rubber LinerW239298 20-400 72 144W239300 24-400 72 144W239301 28-400 72 144W239302 33-400 72 144W239304 43-400 72 72W239306 48-400 72 72

Black Phenolic/PE Poly-Seal® Liner239253 20-400 72 144239257 24-400 72 144239259 28-400 72 144239260 33-400 72 144

White Polypropylene/Teflon® Faced Foamed Polyethylene Liner239229 20-400 72 144239233 24-400 72 144239235 28-400 72 144239236 33-400 72 144239238 43-400 72 72239240 48-400 72 72

White Polypropylene with Poly-Vinyl Liner239205 20-400 72 144239209 24-400 72 144239211 28-400 72 144239212 33-400 72 144239214 43-400 72 72239216 48-400 72 72

Clear, Type III soda-lime glass125-4000 mL (4 oz-130 oz) sizesLargest bottles available Ready-to-use storage container

Standard Wide Mouth Bottles are ideal for general small to large volume storage. Caps available with the choice of two different liners. Bottles are packaged in convenience packs with or without caps attached. Caps may also be purchased separately.




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Standard Wide Mouth Bottle, continued...Cat. No. Capacity (oz) Capacity (mL)* Dia x H (mm) Cap Size Qty/Case






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Straight Sided Jar, continued...Cat. No. Capacity (oz) Capacity (mL)* Dia x H (mm) Cap Size Qty/Case





White Polypropylene/Teflon® Faced Foamed Polyethylene Liner239241 53-400 72 72239242 58-400 72 72239244 70-400 24 48239246 89-400 24 48

White Polypropylene/Poly-Vinyl Liner239217 53-400 72 72239218 58-400 72 72239220 70-400 24 48239222 89-400 24 48

Valumetric Bottle, continued...Cat. No. Capacity (oz) Capacity (mL)* Dia x H (mm) Cap Size Qty/Case

With Black Phenolic Cap/Rubber LinerW216978 1 30 34 x 68 33-400 48W216979 2 60 42 x 83 38-400 48W216980 4 125 51 x 102 48-400 24W216981 8 250 62 x 127 58-400 24


With Black Phenolic Cap/Poly-Seal® LinerW216987 1 30 34 x 68 33-400 432W216988 2 60 42 x 83 38-400 288




Black Phenolic/PE Poly-Seal® Liner239260 33-400 72 144



Clear, Type III soda-lime glassBottles come with cork inserted

Cat. No. Capacity (oz) Capacity (mL)* Dia x H (mm) Qty/Case

W216994 4 125 37 x 163 144


••Clear USP Type III soda-lime glass

30-500 mL (1 oz-16 oz) sizesEasy removal of contentsGraduated in mL and oz

Graduated bottles are ideal for general storage. With the choice of two different liners, these bottles are excellent for storage of liquid or dry materials. They come packaged in convenience packs with caps attached or bulk packs with or without caps. Caps may also be purchased separately.




With Black Phenolic Cap/Poly-Seal® LinerW216976 1 30 34 x 68 33-400 48W216977 2 60 42 x 83 38-400 48

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Wide Mouth Packer, continued...Cat. No. Capacity (oz) Capacity (mL)* Dia x H (mm) Cap Size Qty/Case

With Black Phenolic Cap/Poly-Seal LinerW216951 1 30 37 x 65 28-400 24W216952 2 60 44 x 75 33-400 24W216953 4 125 54 x 95 38-400 24





Black Phenolic/PE Poly-Seal® Liner239259 28-400 72 144239260 33-400 72 144


White Polypropylene/ Poly-Vinyl Liner239211 28-400 72 144239212 33-400 72 144239213 38-400 72 72239215 45-400 72 72239217 53-400 72 72

30-1000 mL (1 oz-32 oz) sizesIdeal for storage of dry powdersEasy removal of contentsFor light-sensitive samplesAmber, USP Type III soda-lime glass

Amber Wide Mouth Packers can be used to store light-sensitive samples. The wide mouth design allows for easier handling of dry or solid samples. These bottles are packaged in convenience packs with caps attached or bulk packs with or without caps. Caps may also be purchased separately.




•••••

Bottles fit most standard hybridization ovensFive bottle sizes accommodate a variety of membrane sizesAcrylic storage rack available separately

•••

BulkBulk

BulkBulk

Use Wheaton Hybridization Bottles in nucleic acid and protein blotting procedures that require a minimum of probe solution. Wheaton hybridization bottles eliminate the need for messy bags and allow hybridization and wash solutions to be quickly poured in and out. They fit many hybridization ovens such as Barnstead, Hybaid and Bellco. We offer a variety of bottle sizes to ensure proper membrane fit.

Our hybridization bottles are manufactured from Wheaton 200 borosilicate glass that conforms to USP Type I requirements and feature heavy 3.2 mm sidewalls for added safety. In addition, safety coated bottles are available with a strong, slip-resistant plastisol coating to reduce potential breakage and exposure to hazardous materials. The 35 mm ID bottle caps are Polybutylene Terephthalate (PBT) with Teflon®/silicone liners. The 70 mm ID bottle caps are Polypropylene with Teflon®/silicone liners. An acrylic rack is also available for safe transporting or laboratory storage. The rack holds up to 6 bottles of size ID 35 mm.

Cat. No. Size ID x L (mm) Cap Size (mm) Qty/Case

Hybridization Bottles805000 35 x 75 45 1805001 35 x 100 45 1805003 35 x 150 45 1805007 35 x 300 45 1805011 70 x 300 70-400 1

Safety Coated Hybridization Bottles805020 35 x 75 45 1805021 35 x 100 45 1805023 35 x 150 45 1805027 35 x 300 45 1805031 70 x 300 70-400 1805015 Acrylic Storage Rack — 1

12


Hand

GripsHand

Grips

125 – 1000 mL size with narrow mouthSpecial pour lip facilitates pouringHand grips on 1 Liter bottle provides easy handlingAvailable with or without graduationsBlack phenolic screw caps with choice of three linersPackaged in partitioned carton

Wheaton Media bottles are manufactured from Wheaton 400 borosilicate glass that conforms to USP Type I requirements and has a low alkali content to help prevent changes in pH to maintain the purity of its contents. The pour lip and sloped shoulders facilitate pouring and cleaning and the hand grips on the 1 Liter bottle makes for easy handling. Bottles come with or without graduations and have a large writing patch. Bottles are available with or without caps. Pre-attached black phenolic screw caps include the following liners:

14B white rubber liner widely used for mixing and storing culture media. Autoclavable.

Polyethylene (LDPE) liner used for distilled water, analytical standards and reagents.

Teflon® faced rubber liner inert to most chemicals. Autoclavable.

••••••

•

•

•

Clear borosilicate glass bottle with graduations and marking patchAmber borosilicate glass bottle in 125 mL size onlyPre-attached black phenolic screw caps with choice of three linersHand grips on 1 L bottle provide easy handling

Cap On Cap Off Cat. No. Capacity (mL) Dia x H (mm) Dia x H (mm) Cap Size Qty/Case

Without Cap219435 125 55 x 123 55 x 119 33-430 48219437 250 67 x 152 67 x 148 33-430 48219439 500 88 x 192 88 x 188 33-430 24219440 1000 103 x 235 103 x 231 38-430 24

With Polyethylene (LDPE) Lined Phenolic Cap219715 125 55 x 123 55 x 119 33-430 48219717 250 67 x 152 67 x 148 33-430 48219719 500 88 x 192 88 x 188 33-430 24219720 1000 103 x 235 103 x 231 38-430 24

With (Autoclavable) Rubber Lined Phenolic Cap 219755 125 55 x 123 55 x 119 33-430 48219757 250 67 x 152 67 x 148 33-430 48219759 500 88 x 192 88 x 188 33-430 24219760 1000 103 x 235 103 x 231 38-430 24

••••

Media Lab Bottles, Graduated, continued... Cap On Cap Off Cat. No. Capacity (mL) Dia x H (mm) Dia x H (mm) Cap Size Qty/Case

With (Autoclavable) Teflon® Faced Rubber Lined Phenolic Cap 219815 125 55 x 123 55 x 119 33-430 48219817 250 67 x 152 67 x 148 33-430 48219819 500 88 x 192 88 x 188 33-430 24219820 1000 103 x 235 103 x 231 38-430 24


Without Cap219845 125 55 x 123 55 x 119 33-430 48

With Polyethylene (LDPE) Lined Phenolic Cap219855 125 55 x 123 55 x 119 33-430 48

With (Autoclavable) Rubber Lined Phenolic Cap219865 125 55 x 123 55 x 119 33-430 48

With (Autoclavable) Teflon® Faced Rubber Lined Phenolic Cap 219875 125 55 x 123 55 x 119 33-430 48

Media bottles are ideal for holding your bottle top dispenser. For a complete selection of Bottle Top Dispensers see pages 99-100.

See replacement caps on page 13.

13


Borosilicate glass helps prevent pH changesAvailable in 100 mL - 2 L size with wide mouthGraduated with writing patchSpecial pour lip facilitates pouringProvided with or without screw capsColor coded caps can be purchased separately

Wheaton Lab 45 Media Bottles are manufactured from USP Type I borosilicate glass. These versatile bottles have a 45 mm screw thread finish, a large writing area and approximate volume graduations. The wide 45 mm finish facilitates filling and retrieval of contents. Sloping shoulders allow for easy cleaning and complete drainage while the polypropylene pour ring eliminates dripping. The Lab 45 is available with a white polypropylene cap and pour ring pre-attached. Caps and pour rings may be purchased separately. The cap has a built-in wedge shaped sealing ring, eliminating the need for a liner. Lab 45 is also ideal for freeze-drying when used with 224100-503 or 224100-507 slotted stoppers and a 240736 screw cap. The bottles, caps, and pour rings are autoclavable.

Cat. No. Capacity (mL) Dia x H (mm) Cap Size (mm) Qty/Case

Bottles with Caps and Pour Rings Attached219925 100 56 x 109 45 12219927 250 70 x 144 45 12219929 500 86 x 186 45 12219930 1000 101 x 233 45 12219931 2000 137 x 267 45 6

Bottles without Caps or Pour Rings219915 100 56 x 103 45 12219917 250 70 x 138 45 12219919 500 86 x 180 45 12219920 1000 101 X 227 45 12 219921 2000 137 X 261 45 6

45 mm screw cap with or without sealing ringHigh temperature screw caps Caps and stoppers for lyophilizationPour rings

The 45 mm polypropylene screw cap with sealing ring are replacements for caps that are provided with Lab 45 media bottles. For high temperature applications use the PBT (polybutylene terephthalate) screw caps and the ETFE (ethylene tetrafluoroethylene) pour lips which can withstand temperatures up to 180ºC. For lyophilization applications use the solid or open top polypropylene screw cap without the sealing ring and use the black lyophilization stopper.

Cat. No. Description Size (mm) Qty/Case

240726 White PP cap with sealing ring 45 12240726-03 Red PP cap with sealing ring 45 12240726-04 Blue PP cap with sealing ring 45 12240736 White PP cap without sealing ring 45 12240740 White PP cap with Teflon® faced silicone liner 45 12240746 White PP cap with open top 45 12240750 Red PBT cap with Teflon® Faced Silicone Liner 45 10240756 Natural Polypropylene Pour Rings 45 12240760 Red ETFE Pour Rings 45 10224100-503 Halobutyl/60 Black Lyophilization Stoppers 43 12224100-507 EPDM Black Lyophilization Stoppers 43 12

••••••

••••

Clear borosilicate glass bottle without graduationsAmber borosilicate glass bottle in 125 mL size onlyPre-attached black phenolic screw caps with choice of linerHand grips on 1 Liter bottle provide easy handling


Clear Glass Bottle Without Cap219415 125 55 x 123 55 x 119 33-430 48219417 250 67 x 152 67 x 148 33-430 48219419 500 88 x 192 88 x 188 33-430 24219420 1000 103 x 235 103 x 231 38-430 24

Clear Glass Bottle With Polyethylene (LDPE) Lined Phenolic Cap219495 125 55 x 123 55 x 119 33-430 48219497 250 67 x 152 67 x 148 33-430 48219499 500 88 x 192 88 x 188 33-430 24219500 1000 103 x 235 103 x 231 38-430 24

Clear Glass Bottle With (Autoclavable) Rubber Lined Phenolic Cap219575 125 55 x 123 55 x 119 33-430 48219577 250 67 x 152 67 x 148 33-430 48219579 500 88 x 192 88 x 188 33-430 24219580 1000 103 x 235 103 x 231 38-430 24

Amber Glass Bottle without Cap 219885 125 55 x 123 55 x 119 33-430 48

For Wheaton media bottlesBlack phenolic caps with 33 and 38 mm screw threads

Cat. No. Cap Size Qty/Case

With Polyethylene (LDPE) Liner240080 33-430 200240081 38-430 200

With Rubber Liner (Autoclavable) 240280 33-430 200240281 38-430 200

With Teflon® Faced Rubber Liner (Autoclavable)240480 33-430 100240481 38-430 100

Open Top Cap With Gray Chlorobutyl/50 Septa and Flange (Autoclavable)240680 33-430 100240683 33-430 1000

••••

••

For Sterile PET Square Media Bottles and Individually Wrapped Media Bottles see pages 26 and 30.

For Safety Coated Media Bottles see page 17.

Solid Top Cap with Liner Open Top Cap with Septa

14


Borosilicate glass bottle and stopperFeatures no-drip pour lipGround glass stopper fits securelyNarrow mouth ideal for liquidsAutoclavable

Narrow mouth reagent bottles with ground glass stoppers feature a no-drip pour lip for easy pouring of contents. An octagon-shaped ground-glass stopper, which protects contents from airborne contaminants, is included. These bottles are manufactured from borosilicate glass and are available in clear or amber glass. Use amber bottles to protect light-sensitive samples during storage.

Cat. No. Capacity (mL) Dia x H (mm) Stopper Size Qty/Case

Clear Glass BottlesW215235 100 53 x 115 14/23 6215237 250 72 x 145 19/26 10215239 500 88 x 175 24/29 10215240 1000 110 x 215 29/32 10215241 2000 135 x 265 29/32 6215243 5000 175 x 320 45/40 1

Amber Glass BottlesW215255 100 53 x 115 14/23 6215257 250 72 x 145 19/26 10215259 500 88 x 175 24/29 10

•••••

Borosilicate glass bottle and stopperFeatures no-drip pour lipGround glass stopper fits securelyWide mouth ideal for solids and viscous liquidsAutoclavable

Wide mouth reagent bottles with ground glass stoppers feature a no-drip pour lip for easy pouring of contents. The bottles are ideal for solids and viscous liquids. These bottles feature a unique design that makes them easy to clean with a lab brush or in a glassware washer. The ground stoppers are octagon-shaped and interchangeable. The bottle’s specially designed rim will not allow fluids to run down the outside of the bottle. The bottles are made from borosilicate glass.

Cat. No. Capacity (mL) Dia x H (mm) Stopper Size Qty/Case

W216014 50 43 x 90 24/20 6W216015 100 54 x 110 29/22 6216017 250 72 x 140 34/35 10216019 500 89 x 175 45/40 10216020 1000 109 x 215 60/46 10216021 2000 135 x 260 60/46 6

Borosilicate glass bottleFeatures no-drip pour lipPre-attached polypropylene screw cap with Teflon®

linerLarge writing patchAutoclavable

These bottles feature a no-drip pour lip and a large screened writing patch for content identification, eliminating the need for manual label application. The pre-attached polypropylene caps have a solid Teflon® liner. The bottles’ sloping shoulders allow for easy filling, cleaning, and safer pouring without liquid surge. The product is manufactured from Wheaton 400 borosilicate glass that conforms to USP Type I requirements to help prevent changes in pH levels and maintain content purity.

Cat. No. Size (mL) Dia x H (mm) Cap Size Qty/Case

220163 125 55 x 120 33-430 6220223 250 67 x 150 33-430 6220283 500 88 x 190 33-430 6

•••••

•••

••

Wheaton borosilicate glass reagent bottles with ground glass stoppers are ideal when needing an all glass bottle. Bottles are available in clear or amber glass with a narrow

or wide mouth. Bottles are autoclavable.

All Glass

BottleAll Glass

Bottle

15


These reservoirs can be used for a variety of applications including mobile phase and filtration reservoirs and for general sample and buffer storage. The reservoirs have a plastic safety coating with a UV blocking agent on the outside. This coating helps retain the liquid contents and glass fragments in case of breakage and blocks virtually all UV light up to 385 nm. The safety coating is also autoclavable up to 121°C at 15-20 psig for 15-20 minutes. The reservoir is manufactured from Wheaton 33 low extractable borosilicate glass that conforms to ASTM Type I, Class A and USP Type I requirements.

Cat. No. Size (mL) Cap Size (mm) Dia x H (mm) Qty/Case

264710 2000 45 138 x 246 1264711 5000 45 186 x 318 1

45 mm white polypropylene screw cap43 mm Halobutyl/60 stopperFits high capacity reservoirs 264710, 264711Teflon faced silicon liner is ultrasonically bonded

to eliminate toxicity


240736 45 mm Screw Cap without Sealing Ring 12224100-503* 43 mm Halobutyl/60 stopper 12240740 45 mm Screw Cap with Teflon Faced Silicone Liner 12

*43 mm stopper can only be used with 240736 cap

••••

ReusableStrong borosilicate glass to withstand

repeated wet or dry sterilization38, 45 or 51 mm screw threadAutoclavable

••

••

Sterile cap with 0.2 μm Gore-Tex® filterFits 348272 and 348273 bottles

Cat. No. Description Cap Size (mm) Qty/Case

W240751 Vented Cap 45 4W240752 Vented Cap 45 12

••

Ideal as mobile phase or filtration reservoirSafety coated glass bottles provide an

added measure of safetyUV protectant coating

Manufactured from 33 low extractable borosilicate glass

Screw cap size 45 mm

••

••

•

These culture vessels are made to exacting tolerances in order to provide higher cell yields. The glass is specially selected for optical clarity, interior surface smoothness, and the absence of glass imperfections. The neck area is tapered for easy cleaning and removal of cells. Glass wall thickness is 2.6 mm. The wider opening vessels provide easier access to the inside for removing cells or clean-ing. These vessels are made of Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and also ASTM E-438 Type I, Class A requirements.

Dia x Flat Overall Usable Approx Cat. No. Length (mm) Surface Area (cm2) Surface (cm2) Capacity (mL) Qty/Case

Roller bottle with 38 mm deep skirted, black phenolic screw cap with white styrene-butadiene rubber liner (G.P.I. 38-415) 348252 110 x 240 550 680 1380 4348253 110 x 285 700 840 1760 4348256 110 x 480 1320 1500 3450 4348258 110 x 550 1555 1680 4230 4

Roller bottle with 45 mm white polypropylene screw cap with inner sealing ring, and polypropylene pour ring. This bottle can also be used with the Vented Cap (Cat. No. W240751)348272 110 x 240 550 680 1380 4348273 110 x 285 700 840 1760 4

Roller bottle with 51 mm black phenolic screw cap with shallow skirt and white styrene-butadiene rubber liner (G.P.I. 51-400)348522 110 x 270 700 840 1760 4348524 110 x 355 940 1070 2500 4348528 110 x 535 1555 1680 4230 4

Glass Roller

BottleGlass R

oller

Bottle

For a complete listing of Wheaton Roller Culture Apparatus see pages 58-63.

Safety

Safety

CoatedCoated

16


Plastisol coating for safetyMade from clear soda-lime glass that conforms to USP Type III requirementsAvailable with or without caps8, 16 & 32 oz Boston Round style80 oz with jug handle

Dia x H Dia x H Cat. No. Approx Size (oz) cap on (mm) cap off (mm) Cap Size Qty/Case

With Aluminum Foil Lined Black Phenolic Cap220735 16 76 x 172 76 x 170 28-400 24220736 32 96 x 210 96 x 208 33-400 12220738 80* 135 x 295 135 x 291 38-439 6

With Polyethylene LDPE Lined Black Phenolic Cap220745 16 76 x 172 76 x 170 28-400 24220746 32 96 x 210 96 x 208 33-400 12

With Poly-Seal® LDPE Cone Lined Black Phenolic Cap 220755 16 76 x 172 76 x 170 28-400 24220756 32 96 x 210 96 x 208 33-400 12

With Teflon® Faced Rubber Lined Black Phenolic Cap (Autoclavable)220775 16 76 x 172 76 x 170 28-400 24220776 32 96 x 210 96 x 208 33-400 12

Without Cap (Autoclavable)220724 8 62 x 142 62 x 140 24-400 48220725 16 76 x 172 76 x 170 28-400 24220726 32 96 x 210 96 x 208 33-400 12220728 80* 135 x 295 135 x 291 38-439 6

*Manufactured with jug handle

•••••

Promotes safety by retaining contents if bottle is accidentally broken PVC-based plastisol coats exterior of bottle Sizes from 4 oz to 5 gallonClear or amber glassAvailable with or without caps

Create a safe work environment with Wheaton’s safety coated bottles. If a bottle is accidentally broken, the PVC based plastisol coating on the bottle retains the contents long enough for proper disposal. Ideal for lab or field applications, the bottle’s non-slip coating allows for greater ease and stability when handling wet or dry. Most bottles are manufactured from soda-lime glass that conforms to USP Type III requirements. The media and reagent bottles are manufactured from Type I borosilicate glass which conforms to USP Type I requirements.

•••••

Plastisol coating for safetyMade from amber soda-lime glass that conforms to USP Type III requirementsAvailable with or without caps8, 16 & 32 oz Boston Round style4 Liter with jug handle


With Aluminum Foil Lined Black Phenolic Cap220935 16 76 x 172 76 x 170 28-400 24220936 32 96 x 210 96 x 208 33-400 12220939 4L 160 x 340 160 x 336 38-430 4

With Polyethylene (LDPE) Lined Black Phenolic Cap220945 16 76 x 172 76 x 170 28-400 24220946 32 96 x 210 96 x 208 33-400 12220949 4L 160 x 340 160 x 336 38-430 4

With Poly-Seal® LDPE Cone Lined Black Phenolic Cap220955 16 76 x 172 76 x 170 28-400 24220956 32 96 x 210 96 x 208 33-400 12220959 4L 160 x 340 160 x 336 38-430 4

With Teflon® Faced Rubber Lined Black Phenolic Cap (Autoclavable)220975 16 76 x 172 76 x 170 28-400 24220976 32 96 x 210 96 x 208 33-400 12220979 4L 160 x 340 160 x 336 38-430 4

Without Cap (Autoclavable)220924 8 62 x 142 62 x 140 24-400 48220925 16 76 x 172 76 x 170 28-400 24220926 32 96 x 210 96 x 208 33-400 12220929 4L* 160 x 340 160 x 336 38-430 4

*Manufactured with jug handle

•••••

17


Plastisol coating for safetyMade from clear soda-lime glass that conforms to USP Type III requirementsWide mouth for use with solid samplesAvailable with or without caps


With Poly-Vinyl Lined Black Phenolic Cap216627 8 75 x 92 75 x 90 70-400 12216629 16 78 x 147 78 x 146 70-400 12216631 32 95 x 182 95 x 180 70-400 12

Without Cap (Autoclavable)216637 8 75 x 92 75 x 90 70-400 12216639 16 78 x 147 78 x 146 70-400 12216641 32 95 x 182 95 x 180 70-400 12

Plastisol coating for safetyMade from clear borosilicate glass 45 mm white polypropylene screw cap attachedPour ring eliminates drips100 to 1000 mL sizeAutoclavableColor coded caps available separately

Cat. No. Size (mL) Approx Dia x H (mm) Cap Size (mm) Qty/Case

219935 100 56 x 109 45 12W219937 250 70 x 144 45 12219939 500 86 x 186 45 12219940 1000 101 x 233 45 12


240726 White PP, w/ Wedge-Shaped Inner Sealing Ring 45 12240726-03 Red PP, w/ Wedge-Shaped Inner Sealing Ring 45 12240726-04 Blue PP, w/ Wedge-Shaped Inner Sealing Ring 45 12

••••

•••••••

Plastisol coating for safetyMade from clear borosilicate glass that conforms to USP Type I requirements125 & 250 mL sizeProvided with graduations and writing patch33-430 mm screw cap can be purchased separatelyAutoclavable

Cat. No. Size (mL) Approx Dia x H (mm) Cap Size Qty/Case

219455 125 55 x 119 33-430 48219457 250 67 x 148 33-430 48

Available with your choice of three liners 14B rubber lined caps autoclavable

Cat. No. Cap Size Liner Qty/Case

240280 33-430 14B White Rubber Liners 200240080 33-430 Polyethylene (LDPE) Liners* 200240480 33-430 PTFE Faced (14B) Styrene-Butadiene 100

* Not autoclavable

Plastisol coating for safetyClear borosilicate glass with writing patch33-430 screw cap attachedPolypropylene cap with Teflon® liner125 & 250 mL sizeAutoclavable

Cat. No. Size (mL) Approx Dia x H (mm) Cap Size Qty/Case

221014 125 58 x 122 33-430 6221017 250 69 x 151 33-430 6

Plastisol coating for safetyMade from low extractable borosilicate glass Serum finish takes rubber stopper closure

Cat. No. Size Approx Dia x H (mm) Stopper Size Qty/Case

221029 5 gallon 294 x 502 12 1

••••••

••

••••••

•••

Safety

Safety

CoatedCoated

18


Alternative to serum bottles and vialsCan be used for lyophilizationFits with I-Loc™ closure or screw cap with stopperSuperior chemical resistanceAutoclavable

Screw Neck diagnostic bottles are an alternative to serum bottles and vials. Each bottle has a controlled ID which allows for fit with the I-Loc™ closure or with a 20-400 screw cap with thin flange stopper. The product is made from Wheaton 400 borosilicate glass that conforms to USP Type I requirements.

Cat. No. Capacity (mL) Dia x H (mm) Cap Size Qty/Case

Clear219360 5 21 x 48 20-400 480219361 10 25 x 61 20-400 480219362 20 32 x 66 20-400 480219364 50 42 x 80 20-400 288

Amber219370 5 21 x 48 20-400 480219371 10 25 x 61 20-400 480219372 20 32 x 66 20-400 480219374 50 42 x 80 20-400 288

For use with Screw Neck Diagnostic BottlesAdvantages of an aluminum seal with the convenience of a screw capPolypropylene screw cap with gray bromobutyl/50 stopperAutoclavable

These closures are specifically designed for diagnostic applications and offer all the advantages of an aluminum seal and stopper with the convenience of a screw cap. The unique combination of a stopper and screw cap ensures excellent sealing characteristics making them ideal for liquid reagents. The contents can be accessed using a syringe through the stopper or with a pipette after removing the screw cap. These polypropylene closures are available in black, white, red, blue, and yellow. Tolerances in the stoppers are maintained to +/-.010.

Cat. No. Color Cap Size Qty/Case

240676-01 Black 20-400 100240676-02 White 20-400 100240676-03 Red 20-400 100240676-04 Blue 20-400 100240676-05 Yellow 20-400 100

•••••

••••

Solid Top Screw Cap Open Top Screw Cap

Polypropylene screw caps in 5 colorsUse with 224100-203 thin flange stopperScrew cap size 20-400Autoclavable

Use these unlined polypropylene caps with screw neck diagnostic bottles. These solid and open topped caps come in five colors and can be used with thin flange lyophilization stoppers. Screw cap thread size 20-400.

Cat. No. Color Qty/Case

Solid Top Screw Caps240706-01 Black 300240706-02 White 300240706-03 Red 300240706-04 Blue 300240706-05 Yellow 300

Open Top Screw Caps 240716-01 Black 300240716-02 White 300240716-03 Red 300240716-04 Blue 300240716-05 Yellow 300

Use with screw caps for Screw Neck Diagnostic BottlesGray chlorobutyl/55 3-leg lyophilization stopperAutoclavable

Use this gray chlorobutyl/55 three-leg lyophilization stopper with Wheaton screw neck diagnostic bottles, in conjunction with open top caps (240716-01 series) or solid top caps (240706-01 series).

Cat. No. Stopper Size Qty/Case

224100-203 20 mm 300

••••

•••

NewNew

19


Made from Type I borosilicate glassLarge capacity 250 & 500 mLGraduations in mLAccepts standard stoppers and seals

These bottles are manufactured from Wheaton 400 borosilicate glass that conforms to USP Type I requirements. Raised graduations indicate approximate capacities in milliliters.

Cat. No. Size (mL) Mouth ID x OD (mm) Dia x H (mm) Qty/Case

223950 250 15 x 30 60 x 158 35223952 500 15 x 30 75 x 190 24

Made from low extractable borosilicate glassSpecial design adds strength for freeze-drying applicationsShrink-wrapped modules reduce particulate contamination

The blowback feature in the neck and specially designed bottom radius, for added strength, are important for lyophilization applications. The clear vials are manufactured from low extractable borosilicate glass that conforms to ASTM Type I, Class A and USP Type I requirements. The amber vials are manufactured from low extractable borosilicate glass that conforms to USP Type I requirements. Vials are packaged in shrink-wrapped modules to greatly reduce particulate contamination.

Catalog No. Size (mL) Mouth ID x OD (mm) Dia x H (mm) Fits Rack Qty/Case

Clear Tubing Vials223683 2 7 x 13 15 x 32 868804 144 223684 3 7 x 13 17 x 38 868810 144 223685 5 13 x 20 22 x 40 868805 144 223686 10 13 x 20 24 x 50 — 144 223687 20 13 x 20 30.5 x 58 — 120

Amber Tubing Vials223693 2 7 x 13 15 x 32 868804 144 223695 5 13 x 20 22 x 40 868805 144223696 10 13 x 20 24 x 50 — 144

3 mL size used extensively for diluent solutionsAccepts rubber stoppers and aluminum sealsAutoclavable at 121°C for 20 minutes


224007 3 7 x 13 17 x 38 500

••••

•••

•••

Fits most lyophilization applications Borosilicate glass conforms to USP Type I requirementsShrink-wrapped partitioned modules reduce breakage

Serum bottles are ideal for long and short term sample storage, lyophilization and vaccine/injectable drug containers. These molded bottles have excellent wall strength and chemical tolerances, which are important for storage applications. Bottles are manufactured from Wheaton 400 borosilicate molded glass that conforms to USP Type I requirements. Bottles are package in shrink-wrapped partitioned modules with the exception of 2 mL size, which has no partition.

Cat. No. Size (mL) Mouth ID x OD (mm) Dia x H (mm) Fits Rack Qty/Case

Clear Serum Bottles223712 2 7 x 13 17.5 x 35 868805 288223738 5 13 x 20 23 x 47 868805 288223739 10 13 x 20 25 x 54 868806 288223742 20 13 x 20 32 x 58 868615 288223743 30 13 x 20 37 x 63 868615 288223745 50 13 x 20 43 x 73 868615 288223746 60 13 x 20 41 x 91 868615 144223747 100 13 x 20 52 x 95 868617 144223748 125 13 x 20 54 x 107 — 144

Amber Serum Bottles223760 5 13 x 20 23 x 47 868805 288223761 10 13 x 20 25 x 54 868806 288223762 20 13 x 20 32 x 58 868615 288223763 30 13 x 20 37 x 63 868615 288223764 50 13 x 20 43 x 73 868615 288223766 100 13 x 20 52 x 95 868617 144

Note: Select aluminum seal and stopper size by using the mouth OD dimension of the bottle or vial.

•••

For Crimpers and Decappers see pages 35-36.

Conforms to USP Type I Requirements

Borosilicate

GlassBorosilic

ate

Glass

PP Serum Bottle HDPE Serum Bottle

20


Uni-Dose Glass Bottle Uni-Dose PET Vial

Available in amber glass or amber PETMouth OD 28.5 mmUse with Wheaton tamper evident safety cap

These products feature a large labeling surface for easy product identification. The bottles are made from Wheaton 900 soda-lime amber glass that conforms to USP Type III requirements and are packaged in convenient trays of 100. The plastic PET (Polyethylene Terephthalate) vials provide an alternative to glass and they cost less to ship. Use the 224316 Crimper to secure the tamper-evident safety caps (Cat. No. 224195) to the bottle or vial. Tamper-evident safety caps can be purchased separately. For sterilization information, see our Technical Data Section.

Cat. No. Size (mL) Dia x H (mm) Trays/Case Qty/Case

Amber Glass Bottle226732 15 32 x 42 5 500226733 30 36 x 58 5 500226734 60 43 x 83 2 200

Amber PET Vial226772 15 32 x 42 450226773 30 34 x 55 300

For use with Wheaton Uni-Dose® Bottles and Vials

These three-piece lined aluminum caps have unique metal inserts. Contents cannot be contaminated without removing cap or leaving evidence of puncture or insert. A metal tab with a rolled safety edge "perks up" to provide visual proof of tight seal. The caps remove quickly and easily in one piece. They fit all Wheaton Uni-Dose bottles and vials. Tamper-Evident Safety Cap catalog number 224196 is stamped inside "For Oral Use." Safety Caps can be attached to Uni-Dose Bottle & Vials by using the Wheaton Crimper (Cat. No. 224316).

Cat. No. Description For Mouth OD (mm) Qty/Case

224195 Cap for Uni-Dose Bottle and Vial 28.5 2500224196 Cap with “For Oral Use” Imprint 28.5 5000224316 Crimper 28.5 1

•••

•

Serum Bottles, continued...

15, 30, 60 & 120 mL size Manufactured from high density polyethyleneWidely used for animal health products and storage of non-medical productsAccepts standard rubber stoppers and aluminum seals


224031 15 13 x 20 28 x 56 300224033 30 13 x 20 32 x 67 200224036 60 13 x 20 38 x 84 100224037 120 13 x 20 47 x 103 100

Create a dropper bottle using a serum bottle with 20 mm OD

Dropper tip is made from PVC with PP capDispenses 40 μL drop using water

The Dropper Tip is made from PVC with a white polypropylene overcap to protect contents and fits all 20 mm serum finish containers. It provides

an economical way to convert a glass serum bottle to a dropping bottle where glass packaging is mandatory. The dropper dispenses 40 μL per drop when using distilled water at room temperature.


224080 PVC Dropper Tip 23 x 44 100

4 to 120 mL sizePre-attached low density polyethylene snap caps

These bottles are ideal for collecting, storing, and exhibiting a wide variety of specimens in the lab or field. The caps snap over a special sealing ring to form an effective moisture barrier. The bottles are packed in a carton constructed of corrugated trays with partitions. They are made from Wheaton 800 soda-lime clear glass that conforms to USP Type III requirements.


225532 4 23 x 27 22 144225534 8 22 x 39 22 144225535 12 22 x 51 22 144225536 16 30 x 40 30 144225538 24 30 x 52 30 144225539 30 30 x 63 30 144225540 37 31 x 73 30 72225541 44 32 x 83 30 72225543 30 37 x 50 34 72225544 60 45 x 60 45 72225546 120 50 x 95 45 72


242612 22 200242615 30 200242616 34 200242619 45 200

••••

•

••

••

For Aluminum Seals & Rubber Stoppers for serum bottles and vials see pages 32-34. Also see the new Wheaton Premium Stoppers on page 33.

21


P lastic containers have been developed for a variety of applications across many different industries over the years. There are many different types of Polymers used in the creation of these containers to help fill the demands for the various applications.

Polymers offer a variety of properties, each having different levels of importance with different users depending on the application. Some users may have flexibility within their product formula or filling process and therefore focus on economical containers while others may need containers that are stronger, autoclavable, transparent, sterilized, etc., therefore requiring more specifications. Wheaton Science Products can help with polymer selection through comprehension of the customer’s product, goals, and adaptability. Several questions should be posed to gather this understanding. Some examples include:

What is the container size and physical design. Narrow mouth vs. wide mouth, tall vs. short, etc.?

Must the package be transparent, translucent, opaque or colored for either marketing or light protection?

Are there specific shipment and storage conditions such as refrigeration, freezing, exclusion of light, etc.?

Are there governmental regulations pertaining to the product?

How is the product going to be dispensed by the user?

Have any tests been run in plastic? Was it unsuccessful and why? What type of plastics?

•

•

•

•

•

•

Many things govern polymer suitability for package use. These include:

Permeation/BarrierSorption CharacteristicsChemical ResistanceStress Crack ResistanceRigidity/FlexibilityImpact ResistanceSterilizabilityRecyclabilityTemperature Resistance

•••••••••

22


High Density Polyethylene (HDPE)Flexible but more rigid than LDPE. Natural color is milky white, semi-translucent depending on density. Good impact strength and stress crack resistance. Good chemical resistance. Good vapor barrier but poor gas barrier. Sterilizable via ethylene oxide (EtO) or gamma radiation. SPI resin identification code number

Low Density Polyethylene (LDPE)Very flexible, natural milky color, translucent with high impact strength. Excellent for mild and strong buffers, good chemical resistance. Good water vapor and alcohol barrier properties. Poor gas barrier, sterilizable with EtO or gamma radiation. Good stress crack and impact resistance. SPI resin identification code number

Polyethylene Terephthalate (PET)Semi-rigid to rigid depending on wall thickness. Natural color - clear and transparent. Good alcohol and solvent barrier; good gas and fair moisture barrier. Good to fair chemical barrier; not good for strong acids or bases. Good moldability. Sterilizable through EtO and gamma radiation. Good stress crack and impact resistance at room temperature and above. SPI resin identification code number

Polypropylene (PP)Rigid, solid, durable in container or cap forms. Opaque, natural grayish yellow in natural form. Excellent stress crack and impact resistance. Excellent moisture barrier, good oil and alcohol barrier, poor gas barrier properties. Good chemical resistance. Sterilizable with EtO or autoclaving. SPI resin identification code number

Polystyrene (PS)PS is a transparent, rigid and glasslike polymer. Good resistance to inorganic chemicals. Light and heat stable, biologically inert and non-toxic. Poor impact and stress crack resistance, poor barrier properties. EtO or gamma sterilizable. SPI resin identification code number

Ideal for reagents and buffer solutionsSterilized and sold with white caps attachedClear and amber availableA variety of sterile, polypropylene caps are offered separately

Wheaton PET diagnostic bottles and caps are gamma radiation sterilized. These bottles are ideal for storage and shipment of reagents and buffer solutions. They are lightweight and break resistant. They come with pre-attached white polypropylene caps lined with F217 foamed LDPE. The bottles are packaged in 20 per module and 5 modules per case. Sterile, colored caps can also be purchased separately. Assorted caps come with 1 pack each of blue, red, yellow, green, and black caps in a case of 100. Bottles and caps can be autoclaved.*

Cat. No. Size (mL) Color Qty/Pk Qty/Cs

ClearW219981 5 Clear 20 100W219982 10 Clear 20 100W219983 20 Clear 20 100

AmberW219985 5 Amber 20 100W219986 10 Amber 20 100W219987 20 Amber 20 100

Polypropylene Screw CapsCat. No. Cap Size (mm) Color Qty/Pk Qty/Cs

W219990 20-410 Blue 20 100W219991 20-410 Red 20 100W219992 20-410 Yellow 20 100W219993 20-410 Green 20 100W219994 20-410 Black 20 100W219995 20-410 White 20 100W219996 20-410 Assorted 20 100

*Autoclavable for 15 minutes at 121ºC at 15 psi.

Made from Low Density PolyethyleneTranslucent colorVery flexible with high impact strengthExcellent for mild and strong buffers,

good chemical resistanceRaise the spout to dispense contents

and depress spout to seal bottle60 – 500 mL capacity

Cat. No. Size (mL) Dia x H (mm) Cap Size Qty/Pack Qty/Case

211194 60 39 x 92 20-410 12 144211195 125 43 x 130 20-410 12 72211199 500 68 x 180 28-410 6 36

••••

••••

•

•

Resin Identification CodesWheaton follows the Society of Plastics Industry guidelines for marking plastic containers with the appropriate code numbers as shown below:

Wheaton marks their containers on the bottom with the appropriate resin recycle code.

PETE HDPE V LDPE

PP PS OTHER

for more information on chemical compatibility, properties

of plastics, permeability of plastics, and sterilization of plastics.

NewNew

23


Bottle made of natural or white LDPE (Low Density Polyethylene) White LDPE bottle helps protect UV light sensitive samplesPackaged in cases of 100, 1000 or bulk

Size (mL) Cap Size Cat. No. Cat. No. Cat. No. Qty

White Dropping Bottles 100/Case 1,000/Case Bulk/Case Bulk/Case1 8-425 W242830 W242830-A W242830-X 30003 8-425 W242831 W242831-A W242831-X 30006 13-425 W242832 W242832-A W242832-X 24007 15-415 W242833 W242833-A W242833-X 180010 15-415 W242834 W242834-A W242834-X 140015 15-415 W242835 W242835-A W242835-X 800 30 20-410 W242836 W242836-A W242836-X 150060 20-410 W242837 — W242837-X 800125 20-410 W242838 — W242838-X 450

Natural Dropping Bottles 100/Case 1,000/Case Bulk/Case Bulk/Case1 8-425 W242820 W242820-A W242820-X 30003 8-425 W242821 W242821-A W242821-X 30006 13-425 W242822 W242822-A W242822-X 26007 15-415 W242823 W242823-A W242823-X 180010 15-415 W242824 W242824-A W242824-X 140015 15-415 W242825 W242825-A W242825-X 800 30 20-410 W242826 W242826-A W242826-X 150060 20-410 W242827 — W242827-X 800125 20-410 W242828 — W242828-X 450

•••

Wheaton LDPE (Low Density Polyethylene) Dropping Bottles are available in natural or white to protect UV light sensitive products. The controlled dropping tip is easy to attach and remove from the bottle. Dropping bottles, tips and caps are manufactured to strict quality control standards to ensure the best product for your application. Many different colors and sizes are available to meet your requirements. Special sizes, colors, resins and configurations are available upon request.

Controlled dropper tips are tips which have their orifices pierced during a secondary operation. A .020 inch needle is used to pierce the tips. The general tolerance for the hole size in a pierced part is +/- 0.003 inches. The average drop size for stock, controlled round tips (8 mm – 20 mm) is 41 μL +/- 5 μL*. The Wheaton line of dropper bottles, tips and caps offer quality and versatility in a variety of dispensing applications.*Average drop size was calculated through statistical analysis of randomly selected dropper tips using distilled water at room temperature of approximately 78ºF.

24


Tip made of LDPE (Low Density Polyethylene) Extended controlled dropper tip dispenses one drop at a timeAverage drop size is 41 μL +/- 5 μL Packaged in case of 100, 1000 or bulk

Color Cat. No. Cat. No. Cat. No.

8 mm Tip 100/Case 1,000/Case 20,000/Case Natural 242401-01 W242401-01-A W242401-01-XPink 242401 W242401-A W242401 -X Red 242402 W242402-A W242402 -X Orange 242403 W242403-A W242403 -X Blue 242404 W242404-A W242404 -X Gray 242405 W242405-A W242405 -X Green 242406 W242406-A W242406 -X Purple 242407 W242407-A W242407 -X Yellow 242408 W242408-A W242408 -X Brown — — — White 242410 W242410-A W242410 -X

13 mm Tip 100/Case 1,000/Case 10,000/CaseNatural 242411-01 W242411-01-A W242411-01-XPink 242411 W242411-A W242411 -X Red 242412 W242412-A W242412 -X Orange 242413 W242413-A W242413 -X Blue 242414 W242414-A W242414 -X Gray 242415 W242415-A W242415 -X Green 242416 W242416-A W242416 -X Purple 242417 W242417-A W242417 -X Yellow 242418 W242418-A W242418 -X White 242420 W242420-A W242420 -X

15 mm Tip 100/Case 1,000/Case 9,000/CaseNatural 242421-01 W242421-01-A W242421-01-XPink 242421 W242421-A W242421 -X Red 242422 W242422-A W242422 -X Orange 242423 W242423-A W242423 -X Blue 242424 W242424-A W242424 -X Gray 242425 W242425-A W242425 -X Green 242426 W242426-A W242426 -X Purple 242427 W242427-A W242427 -X Yellow 242428 W242428-A W242428 -X Brown — — — White 242430 W242430-A W242430 -X

20 mm Tip 100/Case 1,000/Case 5,000/Case Natural 242431-01 W242431-01-A W242431-01-XPink 242431 W242431-A W242431 -X Red 242432 W242432-A W242432 -X Orange 242433 W242433-A W242433 -X Blue 242434 W242434-A W242434 -X Gray 242435 W242435-A W242435 -X Green 242436 W242436-A W242436 -X Purple 242437 W242437-A W242437 -X Yellow 242438 W242438-A W242438 -X White 242440 W242440-A W242440 -X

••••

Screw cap made of radiation grade polypropyleneVariety of colorsPackaged in case of 100, 1000 or bulk

Color Cat. No. Cat. No. Cat. No.

Size 8-425 100/Case 1,000/Case 26,000/CaseNatural 242501-01 W242501-01-A W242501-01-XPink 242501 W242501-A W242501 -X Red 242502 W242502-A W242502 -X Orange 242503 W242503-A W242503 -X Blue 242504 W242504-A W242504 -X Gray 242505 W242505-A W242505 -X Green 242506 W242506-A W242506 -X Purple 242507 W242507-A W242507 -X Yellow 242508 W242508-A W242508 -X Brown 242509 W242509-A — White 242510 W242510-A W242510 -X

Size 13-425 100/Case 1,000/Case 15,000/CaseNatural 242531-01 W242531-01-A W242531-01-XPink 242531 W242531-A W242531 -X Red 242532 W242532-A W242532 -X Orange 242533 W242533-A W242533 -X Blue 242534 W242534-A W242534 -X Gray 242535 W242535-A W242535 -X Green 242536 W242536-A W242536 -X Purple 242537 W242537-A W242537 -X Yellow 242538 W242538-A W242538 -X White 242540 W242540-A W242540 -X

Size 15-415 100/Case 1,000/Case 8,000/CaseNatural 242511-01 W242511-01-A W242511-01-XPink 242511 W242511-A W242511 -X Red 242512 W242512-A W242512 -X Orange 242513 W242513-A W242513 -X Blue 242514 W242514-A W242514 -X Gray 242515 W242515-A W242515 -X Green 242516 W242516-A W242516 -X Purple 242517 W242517-A W242517 -X Yellow 242518 W242518-A W242518 -X Brown 242519 W242519-A — White 242520 W242520-A W242520 -X

Size 20-410 100/Case 1,000/Case 4,500/CaseNatural 242521-01 W242521-01-A W242521-01-XPink 242521 W242521-A W242521 -X Red 242522 W242522-A W242522 -X Orange 242523 W242523-A W242523 -X Blue 242524 W242524-A W242524 -X Gray 242525 W242525-A W242525 -X Green 242526 W242526-A W242526 -X Purple 242527 W242527-A W242527 -X Yellow 242528 W242528-A W242528 -X White 242530 W242530-A W242530 -X

•••

See page 25 for dropping bottles, tips, and caps packaged together.

25


Bottle made of LDPE (Low Density Polyethylene) Extended controlled dropper tip contains filterFiltered tip removes particulates when delivering liquidPolypropylene caps packaged separately

The bottles are manufactured from natural LDPE (Low Density Polyethylene). The filter tip has a pore size of 20 to 30 microns. The filter tip is designed to remove particulates when delivering liquids. The average drop size for a filtered tip is 18 μL +/- 1 μL. The white polypropylene screw cap is autoclavable. The bottle is not autoclavable. Dropper bottles, tips and caps are packaged separately.


211590 1 12 x 36 8-425 12 144211592 3 14 x 49 8-425 12 144

Bottle made of LDPE (Low Density Polyethylene) Tip dispenses a stream of liquidRadiation grade polypropylene cap packaged separately

The bottles are manufactured from natural LDPE (Low Density Polyethylene). The tip is designed to dispense a stream of liquid. Use it when a controlled drop is not desired. The tip snaps into place and is easily removed for filling. The white polypropylene screw cap is autoclavable. The bottle is not autoclavable. Dropper bottles, tips and caps are packaged separately.


211602 6 17 x 58 13-425 12 144211603 7 19 x 63 15-415 12 144211604 15 25 x 78 15-415 12 144211605 30 32 x 85 20-410 12 144211606 60 39 x 101 20-410 12 144211607 125 47 x 125 20-410 6 72

••••

•••

Bottle made of LDPE (Low Density Polyethylene) Extended controlled dropper tip dispenses one drop at a timeAverage drop size is 41 μL+/- 5 μL Radiation grade polypropylene screw caps and tips packaged separately

The bottles are manufactured from natural LDPE (Low Density Polyethylene). The white pigmentation in the white dropping bottle helps protect UV light-sensitive samples. The tip is designed to dispense one drop at a time. Average drop size is 41 μL +/- 5 μL. The tip snaps into place and is easily removed for filling. The white polypropylene screw cap is autoclavable. The bottle is not autoclavable. Dropper bottles, tips and caps are packaged separately.


Natural Dropping Bottles211628 1 12 x 36 8-425 12 144 211630 3 14 x 49 8-425 12 144 211631 6 17 x 58 13-425 12 144 211632 7 19 x 63 15-415 12 144 211633 15 25 x 78 15-415 12 144 211634 30 32 x 85 20-410 12 144 211635 60 39 x 101 20-410 12 144 211636 125 47 x 125 20-410 6 72

White Dropping Bottles211618 1 12 x 36 8-425 12 144 211620 3 14 x 49 8-425 12 144 211621 6 17 x 58 13-425 12 144 211622 7 19 x 63 15-415 12 144 211623 15 25 x 78 15-415 12 144 211624 30 32 x 85 20-410 12 144211625 60 39 x 101 20-410 12 144211626 125 47 x 125 20-410 6 72

3 mL LDPE (Low Density Polyethylene) bottlesAssembled with dropper tip and cap attached to bottleAverage drop size is 41 μL +/- 5 μL Packaged in a reusable plastic caseExtended controlled dropper tip dispenses one drop at a time

Vial Files are sized to provide optimum storage in minimum space. Alphanumerical indexing of contents provides quick identification and location of samples. Each case contains 40 vials arranged in four rows of 10.

Cat. No. Size (mL) Dia x H (mm) Cap Size Vials/File Qty/Case

211641 3 14 x 49 8-425 40 1

••••

•••••

Controlled D

ropper

Tip with FilterContro

lled Droppe

r

Tip with Filter

Dispenses S

tream

of LiquidDispenses S

tream

of Liquid

Dispenses

One Drop

at a Time

Dispenses

One Drop

at a Time

See pages 23-24 to purchase dropping bottles, tips, and caps separately.

26


High Density Polyethylene, natural colorGood chemical resistanceNarrow mouth ideal for liquids30 – 500 mL sizesPre-attached white polypropylene screw cap with foamed polyethylene liner

Cat. No. Size (oz) Size (mL)* Dia x H (mm) Cap Size Qty/Pack Qty/Case

221153 1 30 27 x 78 20-410 12 72221154 2 60 35 x 98 20-410 12 72221155 4 125 41 x 123 20-410 12 72221159 16 500 67 x 176 28-410 8 48


High Density Polyethylene, white colorWhite HDPE helps protect light sensitive samplesGood chemical resistanceNarrow mouth ideal for liquids30 – 120 mL sizesPre-attached white polypropylene screw cap with foamed polyethylene liner


221163 30 27 x 78 20-410 12 72221164 60 35 x 98 20-410 12 72221165 120 41 x 123 24-410 12 72

High Density Polyethylene, natural colorGood chemical resistanceNarrow mouth ideal for liquids1 gallon sizePre-attached white polypropylene screw cap with polyvinyl liner

Cat. No. Size Cross Section x H (mm) Cap Size Qty/Case

222333 1 gallon 143 x 285 38-400 4

•••••

••••••

•••••

Low Density Polyethylene, translucent colorLDPE is very flexible with high impact strengthExcellent for mild and strong buffers, good chemical resistanceNarrow mouth ideal for liquids7 – 125 mL sizesPre-attached white polypropylene screw cap with foamed polyethylene liner


221140 7 19 x 63 15-415 12 144221142 15 25 x 78 15-415 12 144221143 30 33 x 73 20-410 12 72221144 60 39 x 88 20-410 12 72221145 125 47 x 109 20-410 12 72

PET Bottle Sterile PET Bottle

Polyethylene Terephthalate, transparentPET offers clarity of glass with the safety of plasticGood alcohol and solvent barrier; not good for strong acids and basesSterilizable through EtO and gamma radiation120 mL cylinder round style, smaller sizes have sloped shouldersNarrow mouth ideal for liquids22 – 120 mL sizePre-attached white polypropylene screw cap with foamed polyethylene liner


221131 22 29 x 64 20-415 12 72221133 45 36 x 76 20-400 12 72221135 120 41 x 120 20-410 12 72

Sterile and individually wrappedExcellent for sensitive cell culture or production samplingGood chemical resistance against mild acids, oils and alcoholPET offers clarity of glass with the safety of plasticNarrow mouth ideal for liquids125 mL sizePre-attached white polypropylene screw cap with foamed polyethylene liner


219955 125 52 x 115 33-430 12

••••••

••••••••

•••••••

Natural Cylinder Round White Cylinder Round

27


High Density Polyethylene, natural Good chemical resistanceBlake design maximizes storage space Wide mouth ideal for solids120 – 1000 mL sizePre-attached white polypropylene screw cap with foamed polyethylene liner

Cat. No. Size (oz) Size (mL)* Dia x H (mm) Cap Size Qty/Case

209683 4 120 41 x 50 x 94 38-400 72209685 16 500 62 x 77 x 149 43-400 48209686 32 1000 79 x 96 x 192 53-400 24


••••••

High Density Polyethylene, natural Good chemical resistanceRound styleWide mouth ideal for solids60 – 1000 mL sizePre-attached white polypropylene screw cap with foamed polyethylene liner

Cat. No. Size (oz) Size (mL)* Dia x H (mm) Cap Size Qty/Case

209672 2 60 39 x 77 33-400 72209673 4 120 50 x 88 38-400 72209674 8 250 65 x 102 53-400 72209675 16 500 77 x 142 53-400 48209676 32 1000 95 x 179 53-400 24


••••••

General Purpose Bottles, continued...

28


Wide mouth design is ideal for large solid samplesSafer to use than glass jarsChoice of resins: HDPE, polypropylene or polystyreneClear polystyrene offers clarity of glass Polypropylene is autoclavable30 to 3840 mL sizeOptional foam-lined caps help prevent leaks

The Wheaton line of wide mouth plastic containers are ideal for use with powders and solids. The large surface area of these containers makes it easy to label, either on the wide-mouth cap or directly on the side of the container. Unlike glass jars, plastic jars will not break easily when bumped or dropped. This makes these jars an excellent choice for lab or field sampling. Only the polypropylene containers are autoclavable.

Durable container with good chemical resistance30 to 1000 mL sizePre-attached white polypropylene screw cap unlined or with foamed

polyethylene linerPolypropylene container with linerless cap is autoclavable

Cat. No. Size (mL) Cap Size Qty/Case

Polypropylene with Linerless Cap Attached (Autoclavable)W209900 30 43-400 72W209901 60 53-400 48W209902 125 58-400 36W209903 250 89-400 36W209904 500 89-400 24W209905 1000 120-400 24

Polypropylene with Polyethylene Lined Cap Attached W209906 30 43-400 72W209907 60 53-400 48W209908 125 58-400 36W209909 250 89-400 36W209910 500 89-400 24W209911 1000 120-400 24

•••••••

•••

•

NewNew

Clear polystyrene offers clarity of glass and safety of plasticGood resistance to inorganic chemicals30 to 1000 mL sizePre-attached white polypropylene screw cap with foamed polyethylene liner

Cat. No. Size (mL) Cap Size Qty/CaseW209912 30 43-400 72W209913 60 53-400 48W209914 125 58-400 36W209915 250 89-400 36W209916 500 89-400 24W209917 1000 120-400 24

HDPE, natural colorGood chemical resistanceLargest container available: 2000 to 3840 mL sizePre-attached white polypropylene screw cap with foamed polyethylene liner

Cat. No. Size (mL) Cap Size Qty/CaseW209677 2000 89-400 6W209678 3000 100-400 4W209679 3840 89-400 4

••••

••••

Resin Identification CodesWheaton follows the Society of Plastics Industry guidelines for marking plastic containers with the appropriate code numbers as shown below:

Wheaton marks their containers on the bottom with the appropriate resin recycle code.

PP PS OTHER

PETE HDPE V LDPE

29


HDPE, LDPE or PolypropyleneAmber or naturalLeak resistant design with double-seal closureNo-drip pour lip for safe and clean pouringCap attached

Premium narrow mouth plastic bottles feature our superior double seal closure, which is designed to be leak resistant. The special no-drip pour lip ensures safe, clean pouring. Bottles are convenience packed with polypropylene closures attached.

Cat. No. Size (oz)* Size (mL) Dia x H (mm) Cap Size Qty/Pack Qty/Case

Low Density Polyethylene, Natural 208923 .3 8 25 x 43 20-410 12 72208924 .5 15 25 x 56 20-410 12 72208925 1 30 32 x 69 20-410 12 72 208926 2 60 39 x 84 20-410 12 72 208927 4 125 50 x 101 24-410 12 72 208928 8 250 61 x 129 24-410 12 72 208929 16 500 72 x 171 28-410 12 48

High Density Polyethylene, Natural209041 .1 4 16 x 38 13-425 12 72 209043 .3 8 25 x 43 20-410 12 72 209044 .5 15 25 x 56 20-410 12 72 209045 1 30 32 x 69 20-410 12 72 209046 2 60 34 x 84 20-410 12 72 209047 4 125 50 x 101 24-410 12 72 209048 8 250 61 x 129 24-410 12 72 209049 16 500 72 x 171 28-410 12 48 209050 32 1000 91 x 210 38-430 6 24

High Density Polyethylene, Amber 209121 .1 4 16 x 38 13-425 12 72 209123 .3 8 25 x 43 20-410 12 72 209124 .5 15 25 x 56 20-410 12 72 209125 1 30 32 x 69 20-410 12 72 209126 2 60 34 x 84 20-410 12 72 209127 4 125 50 x 101 24-410 12 72 209128 8 250 61 x 129 24-410 12 72 209129 16 500 72 x 171 28-410 12 48 209130 32 1000 91 x 210 38-430 6 24

Polypropylene, Natural (Autoclavable)209161 .1 4 16 x 38 13-425 12 72 209163 .3 8 25 x 43 20-410 12 72 209164 .5 15 25 x 56 20-410 12 72 209165 1 30 32 x 69 20-410 12 72 209166 2 60 34 x 84 20-410 12 72 209167 4 125 50 x 101 24-410 12 72 209168 8 250 61 x 129 24-410 12 72 209169 16 500 72 x 171 28-410 12 48 209170 32 1000 91 x 210 38-430 6 24


•••••

HDPE, LDPE or PolypropyleneAmber or naturalLeak resistant design with double-seal closureNo-drip pour lip for safe and clean pouringCap attached

Premium wide mouth plastic bottles feature a superior double seal closure, designed to be leak resistant. The special no-drip pour lip ensures safe, clean dispensing. These bottles are convenience packed with polypropylene closures attached.

Cat. No. Size (oz)* Size (mL) Dia x H (mm) Cap Size Qty/Pack Qty/Case

Low Density Polyethylene, Natural209425 1 30 33 x 69 28-410 12 72209426 2 60 39 x 68 28-410 12 72209427 4 125 51 x 98 38-410 12 72209428 8 250 61 x 125 43-410 12 72 209429 16 500 72 x 164 53-410 12 48

High Density Polyethylene, Natural209545 1 30 33 x 69 28-410 12 72209546 2 60 39 x 88 28-410 12 72209547 4 125 51 x 98 38-410 12 72209548 8 250 61 x 125 43-410 12 72209549 16 500 72 x 164 53-410 12 48 209550 32 1000 91 x 199 63-415 12 48

High Density Polyethylene, Amber209625 1 30 33 x 69 28-410 12 72 209626 2 60 39 x 88 28-410 12 72209627 4 125 51 x 98 38-410 12 72209628 8 250 61 x 125 43-410 12 72209629 16 500 72 x 164 53-410 12 48 209630 32 1000 91 x 199 63-415 12 48

Polypropylene, Natural (Autoclavable)209665 1 30 33 x 69 28-410 12 72209666 2 60 39 x 88 28-410 12 72209667 4 125 51 x 98 38-410 12 72209668 8 250 61 x 125 43-410 12 72209669 16 500 72 x 164 53-410 12 48


•••••

Double Seal ClosureLeak resistant double seal ensures safe transport for samples

No-Drip Pour LipSpecial lip is designed to prevent liquids from spilling down the side while pouring

Color code your leak resistant narrow mouth bottles with Wheaton colored screw caps. See page 42 for ordering information.

30


Permanent in-mold graduationsNo-drip pour lipLightweight20% headspace for additives

Wheaton PET Square Media Bottles are manufactured from polyethylene terephthalate which provides the clarity of glass, the strength of plastic, and a space-saving design. These sterile bottles are tested superior for pH stability, temperature durability, cloning efficiency and cytotoxicity. Graduations: size 125 mL is graduated in 25 mL increments; size 500 mL is graduated in 50 mL increments. The bottles come with caps pre-attached and are shrink-wrapped in convenient trays.

Cat. No. Size (mL) Dimensions (L x W x H) Cap Size Qty/Tray Qty/Case219975 125 53 x 53 x 99 mm 33-430 24 48219979 500 75 x 75 x 175 mm 33-430 12 24

New improved 1 Liter BottleThicker wall weightWide mouth designPermanent in-mold graduationsLinerless closure

The new improved 1 L Sterile PET (polyethylene terephthalate) media bottle’s wall weight has been increased by 30%, providing added handling strength and an increased permeation barrier. The mouth of the bottle has increased from 33 mm to 38 mm, providing greater space for pipette entry, which minimizes the risk of contamination. Our engineers integrated the Wheaton superior leak proof designed, double-seal closure system, found on our HDPE leak-resistant bottles, into the 1000 mL PET Media Square. This improved closure system greatly increases seal integrity and eliminates the risk of contamination from cap lining materials. Graduations: size 1 L is graduated in 100 mL increments.

Cat. No. Size (mL) Dimensions (L x W x H) Cap Size Qty/Tray Qty/Case

W219980 1000 91 x 91 x 220 mm 38-430 12 24

Bottle made of LDPE (Low Density Polyethylene) Includes bottle, atomizer tip, dip tube and screw capScrew cap made from polypropylene15 – 60 mL size

Cat. No. Size (mL) Cross Section x H (mm) Cap Size Shelf/Pack Qty/Case226432 15 19 x 33 x 75 15-415 12 144226433 20 20 x 38 x 83 15-415 12 144226434 35 22 x 46 x 94 18-410 12 144226436 60 27 x 53 x 108 18-410 12 144

••••

•••••

••••

125 & 500 mL Bottle

New Improved 1 Liter Bottle

NewImprov

edNewImprov

ed

Eliminate the need to hand pour plates and tubes by using the automated Omnispense® Plus Dispenser. For more details see page 126.

For a plastic serum bottle see page 19.

For a sterile, individually wrapped PET media bottle, see page 26.

31


Wheaton Science Products completes your package with the right closure. We provide a wide variety of caps, seals and stoppers to ensure a perfect fit for your container. Wheaton offers closures suited for a wide range of applications. Products include:

Aluminum seals ................................... 32-33Complete Coat rubber stoppers ................ 33I-LOC™ Closures ........................................ 42Leak resistant caps .................................. 42Microlink® caps ........................................ 39Rubber stoppers ................................. 33-34Screw thread GPI finish caps ............... 37-42Septa ........................................................ 38Snap caps ................................................. 42Ultra Pure rubber stoppers........................ 33

••••••••••

Screw thread cap sizes are expressed in two numbers, which refer to the cap diameter (in millimeters) and the GPI finish (the top of the container). For example, a size 28-430 cap has a thread diameter of 28 mm, and fits a container with a 430 GPI thread finish. Below is a selection of caps and finishes.

425 400 410 415 350 430

Lab 45

Wheaton I-LOC™ Closure

Double Seal Closure & No-Drip Pour Lip Double Seal

Closure

No-Drip Pour Lip

Glass Serum Finish

Plastic Serum Finish

Snap Cap

New

Material Description ApplicationsPoly-Vinyl General purpose: Suitable for wide range of applications. Chemical

resistance: Good for mild acids, alkalis, solvents, alcohols, oils and aqueous products. Poor for active hydrocarbons and bleaches.

One mil poly-vinyl film bonded to one mil HDPE on a #30 white pulp paper backing. Superior to plain pulp paper because it provides an excellent moisture barrier.


Teflon faced foamed polyethylene liner that offers the excellent chemical resistance of Teflon with the compressibility and sealing properties of polyethylene foam.

Teflon® Faced Foamed Polyethylene

Unique problem solving type of liner. This liner is stress crack resistant and offers superior torque retention and excellent sealing characteris-tics. It is recommended that this liner be tested prior to use for leak seal.

Manufactured from polyethylene (LDPE). The unique cone design provides a wedge type seal that not only seals across the top but also across the inside diameter.

Poly-Seal®


The 14B white rubber lining material consists of homogeneous sulfur cured styrene-butadiene rubber. FDA Status complies with 21CFR 177.26, “Rubber articles intended for repeated use.”


Designed for the ultimate in product safety. PTFE provides totally inert inner seal and surface facing the sample or product. Autoclavable.

The white rubber/0.005" PTFE liner consists of virign PTFE bonded to the white sulfur cured styrene-butadiene rubber. Complies with the FDA 21CFR 177.1550.

Teflon® Faced Rubber

New

32


These seals are ideal for filling or retrieving contents with a syringe and as closures for many instrumentation applications. The 8 mm seal is designed for smaller serum finish vials commonly used in autosamplers.

Cat. No. Color For Mouth OD (mm) Shelf Pack Qty/Case

Open Top Seal Lined with Teflon® Faced Natural Red Rubber224209-01 Natural 8 100 1000224211-01 Natural 11 100 1000224211-05 Blue 11 100 1000224211-06 Red 11 100 1000224211-07 Green 11 100 1000

Open Top Seal Lined with Teflon® Faced Silicone224218-01 Natural 8 100 1000224219-01 Natural 11 100 1000224219-05 Blue 11 100 1000224219-06 Red 11 100 1000224219-07 Green 11 100 1000

Center Disc Tear-Out Seal Lined with Teflon® Faced Natural Red Rubber 224222-01 Natural 13 100 1000224223-01 Natural 20 100 1000

Open Top Seal Lined with PTFE/Silicone/PTFEThis seal is recommended for use in autosamplers. 224231-01 Natural 11 100 1000224231-05 Blue 11 100 1000224231-06 Red 11 100 1000224231-07 Green 11 100 1000

Open Top Seal Lined with Teflon® Faced Gray Butyl224234-01 Natural 8 100 1000224235-01 Natural 11 100 1000

With Pulp/Saran Liners

These three-piece lined aluminum caps have unique metal inserts. Contents can-not be pilfered without removing cap or leaving evidence of puncture or insert. A metal tab with a rolled safety edge “perks up” to provide visual proof of tight seal. The caps remove quickly and easily in one piece. They fit all Wheaton Uni-Dose®

Bottles and Vials (see page 20). Cap 224196 is stamped inside “For Oral Use.”

Cat. No. For Mouth OD (mm) Qty/Case

224195 28.5 2500224196 28.5 5000

We offer unlined aluminum seals in a wide range of styles and sizes, in natural aluminum, blue, red and green.


Open Top Unlined Aluminum Seals 224175-01 Natural 8 — 1000224175-05 Blue 8 — 1000224175-06 Red 8 — 1000224175-07 Green 8 — 1000224176-01 Natural 11 100 1000224176-05 Blue 11 100 1000224176-06 Red 11 100 1000224176-07 Green 11 100 1000224177-01 Natural 13 100 1000224177-05 Blue 13 100 1000224177-06 Red 13 100 1000224178-01 Natural 20 100 1000224178-05 Blue 20 100 1000224178-06 Red 20 100 1000224178-07 Green 20 100 1000

Center Disc Tear-Out Unlined Aluminum SealsThe center disc tears out, exposing the top of the stopper to the syringe. 224182-01 Natural 13 100 1000224182-05 Blue 13 100 1000224182-06 Red 13 100 1000224182-07 Green 13 100 1000224183-01 Natural 20 100 1000224183-05 Blue 20 100 1000224183-06 Red 20 100 1000224183-07 Green 20 100 1000224187-01 Natural 30 100 1000

Solid Top Unlined Aluminum SealsThese seals are excellent for long-term storage of samples. 224189 Natural 11 100 1000224191 Natural 20 100 1000

Tear-Off Unlined Aluminum SealsThis seal can be completely removed and the contents readily poured from bottle. 224192-01 Natural 13 100 1000224192-05 Blue 13 100 1000224192-06 Red 13 100 1000224192-07 Green 13 100 1000224193-01 Natural 20 100 1000224193-05 Blue 20 100 1000224193-06 Red 20 100 1000224193-07 Green 20 100 1000224197-01 Natural 30 100 1000

Open Top Lined Seal Open Top Lined Seal

Center Disc Tear-Out Lined Seal

Open Top Unlined Seal Center Disc Tear-Out Unlined Seal

Solid Top Unlined Seal Tear-Off Unlined Seal

33


Wheaton’s premium stoppers add confidence with purity. There are three new styles to choose from. Two of the styles feature a new rubber formulation that redefines rubber purity. The third style is a stopper that is completely coated with a fluorinated polymer coating. This coating gives the stopper a chemical resistance similar to the chemical resistance of PTFE. As a result, this coating ensures sample purity. These stoppers are a good choice if you are using harsh

chemicals that require high levels of purity.

Straight Plug StopperWheaton’s new Ultra Pure straight plug stoppers are made from a bromobutyl rubber compound containing the highest level of chemical purity. These stoppers are Water for Injection (WFI) compatible. They also have an ultra low level of extractables and they remain stable when exposed to gamma irradiation making them very well suited for Ready-for-Sterilization and Ready-for-Use applications.Lyophilization StopperWheaton’s new Ultra Pure lyophilization stoppers are made from a bromobutyl rubber compound containing the highest level of chemical purity. These stoppers demonstrate a very low level of moisture absorption and a low level of extractables. They are compatible with Water for Injection (WFI) applications and are not suitable for packaging applications. They are free from natural rubber or natural rubber latex. They are ideal for use in most lyophilization applications.

Cat. No. For Mouth OD (mm) Qty/Case

Silicone Septa 224162 13 1000224163 20 1000

Teflon® Faced Silicone Septa224172 13 100224173 20 100224174 30 100

Teflon® Faced Gray Butyl Septa This is a special molded seal with a Teflon® insert. Sealing surfaces of butyl and Teflon® effect a more positive seal than plain Teflon® faced septa. 224168* 20 100

This item is a specially molded septa with a Teflon® inset. The sealing surface of butyl and Teflon® effect a more positive seal than plain Teflon® faced septa.

Flip Cap Seals are easy to use, autoclavable and tamper-evident. Push upward on the red plastic button to access the stopper or septum. There are no sharp metal edges to cut fingers or gloves. Plus, you can remove the Flip Cap button with one hand, freeing the other for the syringe.

Tear Cap Seals are used for center tear-off and complete tear-off applications. The red plastic top flips off easily with pressure from the thumb exposing the aluminum seal with center hole and pull ring. Access contents by syringe or seal can be torn off completely by pulling on the center tear ring. This seal addresses the need for a recyclable product, especially in Europe, which allows the complete separation of plastic, aluminum, rubber and glass after use.

Cat. No. For Mouth OD (mm) Shelf Pack Qty/Case

Flip Cap Seal224202 13 100 1000224203 20 100 1000

Tear Cap Seal 224208 20 100 1000

For Mouth Stopper Cat. No. ID x OD (mm) Style Stopper Material Shelf Pack Qty/Case

W224100-400 7 x 13 Straight Plug Gray Bromobutyl/47 100 1000W224100-401 7 x 13 2-Leg Stopper Gray Bromobutyl/46 100 1000W224100-402 7 x 13 Igloo Stopper Gray Bromobutyl/46 100 1000W224100-405 13 x 20 Straight Plug Gray Bromobutyl/47 100 1000W224100-406 13 x 20 2-Leg Stopper Gray Bromobutyl/46 100 1000W224100-407 13 x 20 3-Leg Stopper Gray Bromobutyl/46 100 1000W224100-408 13 x 20 Igloo Stopper Gray Bromobutyl/46 100 1000

Straight Plug StopperWheaton’s new Complete Coat stoppers are completely coated with a fluorinated polymer that has barrier properties similar to PTFE. The fluorinated polymer coating is all that comes in contact with the sample. Chemical reactions due to unwanted interference with the rubber itself are virtually eliminated. These stoppers are manufactured in a silicone-free environment. A specialized manufacturing process is used to produce extremely low levels of visible and subvisible particles. These stoppers provide users the greatest level of confidence in avoiding interference with samples. For Mouth Stopper Cat. No. ID x OD (mm) Style Stopper Material Shelf Pack Qty/Case

W224100-420 7 x 13 Straight Plug Gray Bromobutyl/39 100 1000W224100-421 13 x 20 Straight Plug Gray Bromobutyl/39 100 1000

NewNew

Tear Cap Seal Flip Cap Seal

Straight Plug

2-Leg

3-Leg Igloo

Silicone Septa Teflon® Faced Gray Butyl Septa

34


Wheaton offers the cleanest, most precise pharmaceutical grade rubber stoppers available for research and parenteral drug applications.

Production equipment is engineered to yield components with exceptional dimensional stability

Manufacturing tolerances for flange heights on stock stoppers are routinely held to ±.010"

A variety of styles and sizes are availableManufactured from the highest quality raw materials availableRubber formulations include gray butyl, natural rubber, and silicone

This all-purpose design for lyophilization applications reduces the possibility of legs sticking to each other.

For Mouth Cat. No. ID x OD (mm) Stopper Material Shelf Pack Qty/Case

224100-092 7 x 13 Gray Chlorobutyl-Isoprene blend/50 100 1000224100-093 7 x 13 Gray Chlorobutyl/50 100 1000224100-192 13 x 20 Gray Chlorobutyl-Isoprene blend/50 100 1000W224100-193 13 x 20 Gray Chlorobutyl/46 100 1000224100-194 13 x 20 Gray Chlorobutyl/50 100 1000224100-282 20 x 28 Gray Chlorobutyl/50 100 1000224100-503 31 x 43 Black Halobutyl/60 – 12224100-507 31 x 43 Black EPDM – 12

The 3-leg design provides stability during lyophilization and resists cocking.

Cat. No. For Mouth ID x OD (mm) Stopper Material Shelf Pack Qty/Case

224100-202 13 x 20 Gray Chlorobutyl/50 100 1000

This 3-leg lyophilization stopper is made from gray chlorobutyl/55 and is used with Wheaton screw neck diagnostic bottles. This stopper can be used with open top caps (240716-01 series) or closed top caps (240706-01 series). Not recommended for use with crimp top caps.


224100-203 13 x 20 Gray Chlorobutyl/55 – 300

Sleeve stoppers are plug stoppers with fold-over skirts. This type of stopper is used on serum vials and other crimp-finish containers that do not require aluminum seals or overcaps. Sleeve stoppers can be used as an injection site for multiple dose entry. These stoppers are manufactured from natural red rubber, a general-purpose rubber formulation that has excellent resealable properties, making it ideal for use in multi-puncture applications.


224100-020 5 x 11 Natural Red Rubber 100 1000224100-060 7 x 13 Natural Red Rubber 100 1000224100-160 10 x 20 Natural Red Rubber 100 1000224100-161 13 x 20 Natural Red Rubber 100 1000224100-320 15 x 30 Natural Red Rubber 100 1000

•

•

•••

This design provides maximum sealing for vials with straight wall glass finish. The straight wall interior finish is typically found in vials from European manufacturers. This style is not recommended for vials with a blow-back feature, which is a small ridge on the interior of the vial’s neck.


224100-030 5 x 11 Natural Red Rubber/40 100 1000224100-070 7 x 13 Natural Red Rubber/40 100 1000224100-072 7 x 13 Gray Chlorobutyl-Isoprene Blend/40 Teflon® Faced 100 1000224100-170 10 x 20 Natural Red Rubber/40 100 1000224100-171 13 x 20 Natural Pink Rubber/48 100 1000224100-172 13 x 20 Natural Red Rubber/45 100 1000224100-173 13 x 20 Gray Chlorobutyl/45 100 1000224100-174 13 x 20 Gray Chlorobutyl-Isoprene Blend/40 100 1000224100-175 13 x 20 Gray Chlorobutyl-Isoprene Blend/40 Teflon® Faced 100 1000224100-177 13 x 20 Black Viton® – 100224100-178 13 x 20 Silicone 100 1000224100-263 19 x 28 Gray Chlorobutyl-Isoprene Blend/40 Teflon® Faced – 100224100-330 15 x 30 Natural Red Rubber/40 100 1000224100-331 15 x 30 Gray Bromobutyl/50 100 1000

This design provides maximum sealing for vials with a blow-back glass finish, which is a small ridge on the interior of the vial’s neck. The snap-on stopper locks into the blow-back ridge, providing a tight seal and keeping the stopper from backing out. This style is not recommended for vials with a straight-walled finish. The blow-back feature is typically found in vials from North American manufacturers.


224100-080 7 x 13 Natural Red Rubber/40 100 1000224100-081 7 x 13 Gray Chlorobutyl/45 100 1000224100-180 13 x 20 Natural Red Rubber/45 100 1000224100-181 13 x 20 Gray Chlorobutyl/45 100 1000224100-342 15 x 30 Gray Bromobutyl/50 100 1000

2-Leg Lyophilization Stoppers

3-Leg Lyophilization Stopper

3-Leg Thin Flange Lyophilization Stopper Sleeve Style

Straight Plug Style

Snap-On Style

Note: To select the proper size stopper for your serum bottle or vial, use the OD dimension of the stopper and bottle/vial.

35


This lightweight, easy-to-use crimper provides lab-scale or small-production-scale performance at a moderate cost, filling the gap between hand-operated machines and fully automatic equipment. They operate on standard laboratory air supply of 45 to 60 psi. Bottles and aluminum seals are automatically aligned to eliminate partially crimped seals. Exhaust air is passed through the foot-operated valve so the crimper can be used inside a sterile hood. A complete unit consists of a crimper, a foot valve mounted on a base plate, and connecting tubing and fittings, all packed in a polyethylene storage case. A stand

(available separately) supports the crimping jaws about 10” (26 cm) above the benchtop so both hands are free to manipulate the bottles. The heavy metal base has nonskid rubber feet. 1 year warranty.

Dimensions (W x D x H): ..................6 ½” x 9” x 14 ½” (16.5 x 22.9 x 36.8 cm) Weight: ......................................................................................20 lb (9.1 kg)Dimensions and weight include unit plus stand.


224381 Attaches 11 mm seals 1224382 Attaches 13 mm seals 1224383 Attaches standard 20 mm seals 1224465 Stand 1

Compatible with standard hand crimpersFunctions using any standard in-house air

supply or compressed air cylinder

This unit enables hand-operated crimpers and decappers to function in a semi-automatic mode. It accepts most standard hand crimpers and decappers, and requires inlet air pressure of 25 to 40 psi, which may be obtained from a compressed air cylinder or your standard in-house air supply. To operate, simply insert the proper crimper or decapper for the type of seal you wish to use, and depress the foot switch. 1 year warranty.

Dimensions (H x D x W): ................ 19 3/4” x 6” x 8-5/8” (50.2 x 15.2 x 22 cm)Weight: ..................................................................................... 13 lb (5.9 kg)

The Crimpmaster® permits setup in 5 easy steps:Attach a piece of 1/4” ID high pressure tubing (not supplied) from the Crimpmaster® foot switch assembly to an adjustable air pressure supply.

Connect supplied tubing to Crimpmaster® and foot switch assembly, and place assembly on floor.

Remove cushioned grips, adjustable stop screw, and handle retainer from the crimper or decapper (if so equipped).

Depress the handles and slide the crimper or decapper into the Crimpmaster®

Adjust the air pressure (25-40 psi) for the desired crimping tightness.


224550 Crimpmaster Crimping Station 1

••

1.

2.

3.

4.

5.

Cushioned ergonomic handle Labeled for quick size identificationPolished crimping jaws for consistent sealing Works with the Wheaton Crimpmaster® Crimping Station

Wheaton E-Z Crimpers™ feature cushioned ergonomic handles that aid in reducing hand fatigue and adds a high degree of comfort for the user. Each crimper size is labeled for quick identification. Other features include polished crimping jaws for consistent sealing, a short handle and redesigned spring assembly that further aids in reducing hand fatigue. For semi-automatic crimping, use the E-Z Crimper with the Wheaton Crimpmaster® Crimping Station. An adjustable stop allows you to determine the tightness of the seal while maintaining consistent crimping. Tapered jaws provide for easier vial access when using racks. E-Z Crimpers™ can be autoclaved at 121°C. Autoclaving may change the appearance and or reduce product life. 2 year warranty.


W225300 Attaches 8 mm Seals 1W225301* Attaches 11 mm Seals 1W225302 Attaches 13 mm Seals 1W225303 Attaches 20 mm Seals 1

*The 11 mm E-Z Crimper is supplied with a plunger insert to give the option of crimping the top of a vial creating an extra tight seal. We recommend this only for standard opening vials.

These crimpers are used for flip cap seals and for aluminum seals sizes 16.5, 28.5, and 30 mm. 1 year warranty.


224322 Crimper, attaches 13 mm Flip Cap Seals 1224321 Crimper, attaches 16.5 mm Seals 1224323 Crimper, attaches 20 mm Flip Cap Seals 1224316 Crimper, attaches 28.5 mm Uni-Dose™ Seals 1224307 Crimper, attaches 30 mm Seals 1224357 Decapper, removes 30 mm Seals 1

••••

36


Cushioned ergonomic handle Labeled for quick identificationWorks with the Wheaton Crimpmaster® Crimping Station

Wheaton E-Z Decappers™ feature a cushioned ergonomic handle that aids in reducing hand fatigue and adds a high degree of comfort for the user. Each decapper size is labeled for quick identification. These durable, lightweight, hand-operated decappers are an economical way to remove seals quickly and easily. To remove a seal, just place the decapper on the seal and squeeze the handles together. For semi-automatic decapping, use the E-Z Decapper with the Wheaton Crimpmaster® Crimping Station. Tapered jaws provide for easier vial access when using racks. E-Z Decappers can be autoclaved at 121°C. Autoclaving may change the appearance and or reduce product life. 2 year warranty.


W225350 Decapper, 8 mm 1W225351 Decapper, 11 mm 1W225352 Decapper, 13 mm 1W225353 Decapper, 20 mm 1

•••

See our new Lyophilization Section on pages 107-110.

Plier Decappers are an inexpensive and easy way to remove seals. They are made of steel with gray plastic-coated handles. To remove a seal, simply secure the seal between the jaws, squeeze the handles and twist the seal. 1 year warranty.


224372 Removes 13 mm Seals 1224373 Removes 20 mm seals 1

37


With 14B rubber linerAutoclavable

These caps have a nontoxic white styrene-butadiene rubber liner, making them ideal for culture work. The Convenience Packs offer caps in smaller quantities.

Cat. No. Cap Size Qty /Pack Qty/Case240206 8-425 — 1000240208 13-425 — 200240209 15-425 — 200240215 18-400 — 500240264 18-415 — 500240216 20-400 — 500240218 24-400 — 500240278 24-430 — 500240280 33-430 — 200240223 38-400 — 200240269 38-415 — 200240281 38-430 — 200240228 51-400 — 100

Convenience PacksW239298 20-400 72 144W239299 22-400 72 144W239300 24-400 72 144W239301 28-400 72 144W239302 33-400 72 144W239303 38-400 72 72W239304 43-400 72 72W239306 48-400 72 72W239308 58-400 72 72

With Teflon® faced (14B) styrene-butadiene rubber linerAutoclavable

These caps are designed to ensure the ultimate in product safety. The liners are white rubber faced with Teflon.


W240406 8-425 — 200240408 13-425 — 200W240820 13-425 — 250W240821 13-425 250 1000240463 15-415 — 200240409 15-425 — 200W240822 15-425 — 250W240823 15-425 250 1000240414 18-415 — 200240415 18-400 — 200240416 20-400 — 100W240824 22-400 — 100W240825 22-400 100 500240418 24-400 — 100W240827 24-400 100 500W242711 24-400 — 200240419 28-400 — 100240421 33-400 — 100240480 33-430 — 100240481 38-430 — 100

••

••

With PE Poly-Seal® linerCat. No. Cap Size Qty/Pack Qty/Case

239249 13-425 72 144239250 15-425 72 144239251 18-400 72 144239253 20-400 72 144239255 22-400 72 144239257 24-400 72 144239259 28-400 72 144240119 28-400 — 100239260 33-400 72 144240121 33-400 — 100240181 38-430 — 100

Bulk239449 13-425 — 13,000 239450 15-425 — 10,000239451 18-400 — 6,500239453 20-400 — 5,500239455 22-400 — 4,700239459 28-400 — 3,100239460 33-400 — 2,300

With aluminum foil faced pulp board linerUse these caps with organic solvents

Cat. No. Cap Size Qty Case

240319 28-400 100240321 33-400 100240381 38-430 200W240382 38-439 200240330 58-400 200

With polyethylene (LDPE) linerUse these caps with distilled water, analytical standards and reagents


240080 33-430 200240081 38-430 200

•

••

••

38


AutoclavableCat. No. For Cap Size (mm) Material Qty/Case

Locking Flange Septa240563 33 Chlorobutyl/50 100

Plain SeptaW240580 8 Red Teflon® Faced Silicone 100W240581 8 Teflon® Faced Silicone 100W240583 13 Teflon® Faced Silicone 100W240593 13 Teflon® Faced Butadiene 100W240574 15 Silicone 100W240584 15 Teflon® Faced Silicone 100W240594 15 Teflon® Faced Natural Red Rubber 100W240585 18 Teflon® Faced Silicone 100W240596 20 Teflon® Faced Natural Red Rubber 100W240586 20 Teflon® Faced Silicone 100W240587 22 Teflon® Faced Silicone 100W240588 24 Teflon® Faced Silicone 100W240598 24 Teflon® Faced Silicone 10/90 100W240599 24 Teflon®/Silicone/Teflon® 100W240590 33 Teflon® Faced Silicone 100I052665 45 Teflon® Faced Silicone for Celstir® Sidearm Cap 100

Cat. No. Cap Size (mm) Cap Material Cap Liner Material Qty/Case

241009 15-425 Urea Metal Foil 1000240804 22-400 Polypropylene Metal Foil 1000241017 22-400 Urea Metal Foil 1000240817 22-400 Urea Polyethylene Disc 1000240917 22-400 Urea Poly Seal Cone 1000241317 22-400 Polyethylene Linerless 1000240805 24-400 Polypropylene Metal Foil 1000241018 24-400 Urea Metal Foil 1000240818 24-400 Urea Polyethylene Disc 1000

•With Gray Chlorobutyl/50 Septa and FlangeAutoclavable

Ideal for use with Wheaton Media Bottles (Catalog No. 219 series, pages 12-13)Cat. No. Cap Size Qty/Case

240680 33-430 100240683 33-430 1000

Without LinerAutoclavable

Ideal for use with *Vacules® (Catalog No. 651905 and 651907).Cat. No. Cap Size Qty Case

239853 22-350 500

*Vacules are listed in the vial section on page 154.

Without LinerAutoclavable


W240506 8-425 200W240507* 8-425 200W240508 13-425 200W240509 15-425 200W240515 18-400 200W240516 20-400 200W240517 22-400 200W240518 24-400 200W240540 33-430 200

*This has a thicker cap wall designed for Shimadzu and other autosamplers.

••

••

••

For a comprehensive listing of liquid scintillation vials see pages 146-148.

39


With Polypropylene/Silicone/ Polypropylene Liner

These white, closed top, autoclavable caps have ultrasonically bonded liners, making them ideal for use with biological media and other sensitive compounds. They are available in convenient Lab Pack quantities as well as larger Case Packs.


Lab Pack240230-01 20-400 48240231-01 24-400 48240232-01 28-400 48240234-01 38-400 24240235-01 38-430 24

Case Pack240230 20-400 600240232 28-400 480240235 38-430 240

With Polypropylene/Silicone/Teflon® Liner

These white, closed top, autoclavable caps have ultrasonically bonded liners, making them ideal for storage and containment of volatile solvents, sensitive compounds, and corrosive chemicals. They are available in convenient Lab Pack quantities as well as larger Case Packs.


Lab Pack240250-01 20-400 48240251-01 24-400 48240252-01 28-400 48240255-01 38-400 24240256-01 38-430 24

Case Pack240250 20-400 600240251 24-400 600240252 28-400 480240256 38-430 240


These white, open top, autoclavable caps have ultrasonically bonded liners, making them ideal for storage and containment of volatile solvents, sensitive compounds, and corrosive chemicals. They are available in convenient Lab Pack quantities as well as larger Case Packs.


Lab Pack240240-01 28-400 48240241-01 33-400 24240242-01 33-430 24240243-01 38-430 24

Case Pack240241 33-400 240240242 33-430 240240243 38-430 240

•

•

•

Cap liners are ultrasonically welded to cap interiors – eliminating the risk of adhesive contamination and bond failure common with autoclaving.

AutoclavableSolvent free – no glueExcellent chemical resistanceExcellent seal and resealabilityExcellent puncture lifeLow compression set

These polypropylene caps are ideal for use with biological media, volatile solvents, corrosive chemicals, and sensitive compounds. Each Microlink® closure consists of a liner ultrasonically welded into the polypropylene cap. The liner consists of a layer of polypropylene (used for welding to the polypropylene of the cap), a layer of silicone rubber (used for taking up imperfections on the vial surface), and then a layer of Teflon® or polypropylene as the sealing surface. This welded bond provides a superior closure system, maintaining a seal through multiple cleaning and/or autoclaving operations. The use of a low- compression-set silicone is ideal for the storage of samples and sensitive materials for prolonged periods of time over wide temperature ranges. Teflon® or polypropylene film on the exposed face of the liner imparts an inert, highly chemical-resistant surface ideal for the containment and storage of volatile solvents, sensitive compounds and corrosive chemicals.


These black, open top, autoclavable caps are ideal for storage and containment of volatile solvents,

sensitive compounds, and corrosive chemicals. The liner is ultrasonically welded to the cap, eliminating the possibility of glue contamination.


240112 33-430 50240114 38-430 50

With Polypropylene/Silicone Liner

Use this black, closed top, autoclavable cap with biological media and test reagents. It comes with an ultrasonically bonded liner.


240106 33-430 50

••••••

•

•

40


Use these caps with Wheaton or other manufacturers' glass bottles.


With Poly-Vinyl Liner239201 13-425 72 144239202 15-425 72 144239203 18-400 72 144239205 20-400 72 144239207 22-400 72 144239209 24-400 72 144239211 28-400 72 144239212 33-400 72 144239213 38-400 72 72239214 43-400 72 72239215 45-400 72 72239216 48-400 72 72239217 53-400 72 72239218 58-400 72 72239219 63-400 24 48239220 70-400 24 48239221 83-400 24 48239222 89-400 24 48

With Poly-Vinyl Liner239401 13-425 — 19,000239402 15-425 — 5,000239403 18-400 — 9,500239407 22-400 — 7,000239409 24-400 — 5,500239412 33-400 — 3,500239413 38-400 — 3,750239414 43-400 — 2,200239415 45-400 — 1,750239416 48-400 — 2,250239417 53-400 — 2,000239418 58-400 — 1,650239420 70-400 — 1,125239421 83-400 — 500239422 89-400 — 500

With Teflon® Faced Foamed Polyethylene Liner239225 13-425 72 144239226 15-425 72 144239227 18-400 72 144239229 20-400 72 144239231 22-400 72 144239233 24-400 72 144239235 28-400 72 144239236 33-400 72 144239237 38-400 72 72239238 43-400 72 72239239 45-400 72 72239240 48-400 72 72239241 53-400 72 72239242 58-400 72 72239243 63-400 24 48239244 70-400 24 48239245 83-400 24 48239246 89-400 24 48


With Teflon® Faced Foamed Polyethylene Liner239425 13-425 — 19,000239426 15-425 — 5,000239427 18-400 — 9,500239429 20-400 — 8,200239431 22-400 — 7,000239433 24-400 — 5,500239435 28-400 — 4,500239437 38-400 — 3,500239438 43-400 — 3,000239439 45-400 — 2,750239440 48-400 — 2,250239442 58-400 — 1,650239443 63-400 — 1,250239444 70-400 — 1,125239445 83-400 — 550239446 89-400 — 450

With Foamed Polyethylene Liner 239273 13-425 72 144239274 15-425 72 144239275 18-400 72 144239277 20-400 72 144239279 22-400 72 144239281 24-400 72 144239283 28-400 72 144239284 33-400 72 144239285 38-400 72 72239286 43-400 72 72239287 45-400 72 72239288 48-400 72 72239289 53-400 72 72239290 58-400 72 72239291 63-400 24 48239292 70-400 24 48239293 83-400 24 48239294 89-400 24 48

With Foamed Polyethylene Liner 242210 15-415 — 200242212 18-400 — 200242214 18-415 — 200242216 20-400 — 200242217 20-410 — 200242218 22-400 — 200242220 24-400 — 200242222 24-410 — 200242224 28-400 — 200242225 28-410 — 200242226 33-400 — 200242228 33-430 — 200

With Foamed Polyethylene Liner239473 13-425 — 19,000239474 15-425 — 5,000239475 18-400 — 9,500239477 20-400 — 8,200239479 22-400 — 7,000239481 24-400 — 5,500239483 28-400 — 4,500239484 33-400 — 3,500239485 38-400 — 3,750239486 43-400 — 3,000239490 58-400 — 1,650239491 63-400 — 900239492 70-400 — 1,000239493 83-400 — 550239494 89-400 — 500

BulkBulk

BulkBulk

BulkBulk

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Replacement caps for Lab 45™ media bottles 45 mm screw threadInner sealing ringAvailable with liner and center holeAutoclavable

These 45 mm non-reactive, autoclavable caps have sure-grips for easy handling. Use these caps with the Wheaton Lab 45™ media bottles or with other media bottles having a 45 mm screw thread finish. Caps with the inner sealing ring provide a leak-resistant seal. Caps without the sealing ring can be used with the 224100-503 stopper for lyophilization applications. The liner in the Teflon® faced silicone lined cap is ultrasonically bonded to eliminate toxicity and to ensure product safety. The cap with the open top can be accessible with a syringe and can also be used with a 43 mm stopper.

Use the snap-on pour ring with polypropylene caps to eliminate dripping of bottle contents while pouring. The ring is manufactured from autoclavable non-reactive polypropylene and is easily snapped onto any Lab 45™ media bottle with a pour lip.

Cat. No. Description Cap Size Qty/Case

240726 White Cap with Inner Sealing Ring 45 12240726-03 Red Cap with Inner Sealing Ring 45 12240726-04 Blue Cap with Inner Sealing Ring 45 12240736 White Cap without Inner Sealing Ring 45 12240740 White Cap with Teflon® Faced Silicone Liner 45 12240746 White Cap with Center Hole 45 12240756 Polypropylene Pour Ring 45 12224100-503 31 x 43 mm Black Halobutyl/60 Stopper — 12224100-507 31 x 43 mm Black EPDM Stopper — 12

With Teflon® faced silicone liner45 mm screw threadWithstands temperatures up to 200°CAutoclavable

PBT screw caps have high heat resistance and can withstand temperatures up to 200°C for short periods of time. Autoclavable.

Use the ethylene-tetrafluorethylene (ETFE) pour ring with a red PBT screw cap. It withstands temperatures up to 200°C and fits any Wheaton bottle with a 45 mm screw thread finish. Autoclavable.


240750 PBT Screw Cap 45 mm 10240760 ETFE Pour Ring — 10

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Screw Cap, Polypropylene Pour Ring & Lyophilization Stopper

PBT Screw Cap ETFE Pour Ring

These autoclavable caps are designed to ensure the ultimate in product safety. The liners are made of a nontoxic white styrene-butadiene rubber and are faced with Teflon®, which offers excellent chemical resistance.

Cat. No. Cap Size Qty/Pack Qty/CaseW240820 13-425 — 250W240821 13-425 250 1000W240822 15-425 — 250W240823 15-425 250 1000W240824 22-400 — 100W240825 22-400 100 500240418 24-400 — 100W240827 24-400 100 500

These white polypropylene autoclavable caps have a bonded Teflon® faced silicone liner making them ideal for storage and containment of volatile solvents, sensitive compounds, and corrosive chemicals. The liners consist of 0.055" (1.4 mm) of silicone and 0.005" (0.14 mm) of Teflon®. Cat. No. Cap Size Qty/Pack Qty/CaseW240830 13-425 — 250W240831 13-425 250 1000W240832 15-425 — 250W240833 15-425 250 1000W240834 22-400 — 100W240835 22-400 100 500W240836 24-400 — 100W240837 24-400 100 500

These polypropylene autoclavable open top caps have a bonded Teflon® faced silicone liner making them ideal for storage and retrieval of volatile solvents, sensitive compounds and corrosive chemicals. The liners consist of 0.055" (1.4 mm) of silicone and 0.005" (0.14 mm) of Teflon®. Cat. No. Cap Size Color Qty/Pack Qty/CaseW242710 13-425 Black — 250W240840 13-425 White — 250W240841 13-425 White 250 1000W240842 15-425 White — 250W240843 15-425 White 250 1000W240844 22-400 White — 100W240845 22-400 White 100 500W240846 24-400 White — 100W240847 24-400 White 100 500

Open top polypropylene glass-filled cap Provides greater heat resistance than other

PP capsTeflon®/silicone septa bonded to capIdeal for EPA samples

The polypropylene is glass-filled to provide extra chemical and heat resistance compared to standard polypropylene. The .005” Teflon®/ 0.120” Silicone septa is bonded to the cap, providing a permanent connection that is ideal for repeat injec-tions. 24-400 screw cap size fits Wheaton 20, 25, 40, and 60 mL sample vials.

Cat. No. Description Cap Size Qty/Pack Qty/Case

W224600 With Bonded Teflon®/Silicone Septa 24-400 100 200

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Black Phenolic Screw Cap Polypropylene Screw Cap Polypropylene Open Top Screw Cap

The New E-Z Ex-Traction Vial provides benefits to users of automated or manual handling and storage systems. See page 143 for more details.

For bottles that fit with a 45 mm screw cap see page 13.

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Colored caps Use these caps with Wheaton Leak Resistant Narrow Mouth Bottles Available in natural, white, red, blue, and greenSee Leak Resistant Bottles on page 29

Cat. No. Cap Color Qty/Pack Qty/Case

13-425 Screw Thread239501 Natural 12 72239501-02 White 12 72239501-03 Red 12 72239501-04 Blue 12 72239501-06 Green 12 72





Polypropylene closures with gray bromobutyl/50 stoppers

For use with Diagnostic BottlesEliminates use of separate

aluminum seal and rubber stopper

Use these with Wheaton glass diagnostic bottles catalog number series 219360 through 219374 (page 18). These closures are specifically designed for diagnostic applications, and offer all the advantages of an aluminum seal and stopper with the convenience of a screw cap. The unique combination of a stopper and screw cap ensures excellent sealing characteristics, making them ideal for liquid reagents. You can access sample contents using a syringe through the stopper or with a pipette after removing the screw cap. The polypropylene closures are available in black, white, red, blue, and yellow. Tolerances in the stoppers are maintained to +/-.010". Both caps and stoppers are autoclavable.



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Unlined for use with *Wheaton Glass Diagnostic BottlesSolid or open top screw capsUse with thin-flange lyophilization stoppers sold separatelyFive colors available


Solid Top Screw Caps240706-01 Black 20-400 300240706-02 White 20-400 300240706-03 Red 20-400 300240706-04 Blue 20-400 300240706-05 Yellow 20-400 300

Open-Top Screw Caps240716-01 Black 20-400 300240716-02 White 20-400 300240716-03 Red 20-400 300240716-04 Blue 20-400 300240716-05 Yellow 20-400 300

Thin-Flange Lyophilization Stopper224100-203 Gray 20 mm 300

* See page 18 for Wheaton Glass Diagnostic Bottles

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Use these caps with Wheaton Snap-Cap sample bottles (page 20).All sizes have tabs for easy removal Natural low density polyethylene

Cat. No. Cap Size (mm) Qty/Case

242612 22 200242615 30 200242616 34 200242619 45 200

Sterile polypropylene cap with 0.2 micron hydrophobic filter

Ideal for use with glass spinner flasks or roller bottles with 45 mm screw thread

Individually wrapped

This vented cap is a single use 45 mm finish cap that is pierced, and the opening is covered with a hydrophobic filter to allow a flask or bottle to “breathe” while eliminating bacterial contamination from this venting. The product allows gas exchange while preventing liquid, water or contaminants from passing through the filter. Cap cannot be autoclaved.


W240751 Vented Cap 4W240752 Vented Cap 12

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Ideal for soft tissueProvided with loose and tight pestleWheaton 33 low extractable borosilicate glassAutoclavable

This tissue grinder is designed to retain a high percentage of cell nuclei and mitochondria in soft tissues or from cell cultures. In operation, the pestle ball is encircled in liquid, which avoids heat buildup by reducing friction. This grinder is ideal for enzyme studies. The initial grinding is performed using the “loose” pestle. The grinding process is completed using the “tight” pestle. This grinder is used by moving the pestle up and down, and works best with cell suspensions or very soft tissue. The mortar has a large reservoir and pouring lip and is supplied with a “loose” pestle and a “tight” pestle. Two complete sets per case.

Tight Pestle “A” Specification: 0.0010-0.0030 in (0.025 - 0.076 mm) Loose Pestle “B” Specification: 0.0035-0.0055 in (0.089 - 0.14 mm)

Cat. No. Size (mL) Mortar, OD x Length (mm) Overall Length (mm) Qty/Case

357538 1 11 x 48 125 2357542 7 13 x 82 175 2357544 15 22 x 94 210 2357546 40 32 x 140 285 2

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Wheaton Tissue Grinders enable primary cells to be prepared from excised tissues, as well as subcellular components from harvested cell packs. Tissue grinders are used to extract DNA, RNA and proteins for blots or other procedures. They are also used to enhance recovery of other cell products. Capacities on the grinders range from 0.1 mL to 55 mL. There are a variety of grinders to suit many tissue types.

Tapered Tissue Grinders are ideal for connective tissue including heart, muscle and lung. These glass-on-glass grinders feature a long taper design that maximizes grinding efficiency.

Potter-Elvehjem Tissue Grinders incorporates a serrated pestle design to disperse homogenate into the mortar cylinder. Standard units with Teflon® pestles are well suited for soft tissue, such as brain and liver.

Tenbroeck Tissue Grinders have a hollow handle that allows packing with ice, making them suitable for tissue such as liver, intestines and heart.

Dounce Tissue Grinders are supplied with two types of pestles: a loose-fitting pestle for sample reduction and a second, tighter pestle for complete homogenization. The ball-shaped pestles leave cell nuclei intact in soft tissues.

The Wheaton Micro Tissue Grinder Kit offers a complete selection of micro tissue grinders.

All Wheaton tissue grinders are manufactured from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements and replacement parts can be purchased separately.

Wheaton Science Products supplies tools to prepare single cells from tissues to large-scale production of cells in stirred vessels.

Typically, cellular tissue is derived from sources such as an egg, embryo, animal organ such as a spleen, or plant tissue. Wheaton tissue grinders (pages 43-46) reduce excised tissue to primary cells, as well as prepare subcellular components from harvested cell packs. The goal is to create and establish primary cell lines which can be used for production and/or ultimately become a master and/or working cell bank. Wheaton Tissue grinders are also used to recover proteins, DNA and RNA for blots and other procedures.

Wheaton trypsinizing flasks (page 50) are used to further reduce tissue to cellular components. Place tissue in flask, add trypsin and stir the vessel using the Wheaton Micro-Stir® magnetic stirrer (page 53). The deep-fluted flask design adds turbulence; a dam below the sidearm retains un-trypsinized tissue when decanting. Dissociated cells can then be collected. Trypsinizing flasks are also useful when removing cells from microcarrier beads.

Single cells can then be used to start a seed culture in a flask or multiple flasks. After reaching a specific density, inoculate a suspension culture flask (pages 51-52) or roller bottle (page 15) for high volume cell production.

Wheaton Science Products offers large scale cell production systems for the culture of mammalian or insect cells. Maximum control of pH, dissolved oxygen, temperature, agitation and antifoam is provided by these bench-top systems. Unlike common tissue culture flasks, which cannot provide total environmen-tal control, Wheaton cell production systems maximize conditions on the bench without the need for an incubator.

The Cell Optimizer System® (page 54) is a small volume cell culture reactor which incorporates sophisticated control technologies typically found in large production culture systems. The Cell Optimizer System allows determination of cell growth and optimum production conditions prior to large scale culture. Operating parameters can be fine-tuned rapidly in several vessels when working at a scale from 250 milliliters to 3 liters.

The Overdrive™ culture system vessels hold up to 45 liters and provide production at a lower cost (page 56). This system is versatile for suspension or microcarrier cultures and can be combined with controllers and probes.

The Mini-Pilot Plant™ bioreactor provides total environmental control with 10,19 or 45 liter vessels operating on the bench-top. Incubators are eliminated with the innovative heating blanket. Probes, pumps, controllers and optional computer interface are available. Large scale cell production is afforded at a fraction of the cost of comparable bioreactor systems.

In culture, anchorage dependent cells want to be attached to a surface wall of a culture vessel or microcarrier beads suspended in culture media. Motionless methods of monolayer cell culture usually consist of T flasks which lay flat. Cells will grow on the inside flask surface only where the media covers them. Since adherent cells usually demonstrate contact inhibition, cell growth is restricted to the amount of contact surface area of the flask. This severely limits the static method being used for large scale production of cells.

As an alternative to T flasks, Wheaton Science Products offers various roller apparatus (pages 58-63) and options to allow for maximum flexibility. This allows the entire surface of a roller bottle to be used to grow monolayer cells without filling the entire bottle with expensive media. Using the Wheaton Roller Apparatus, increases surface area which equals larger cell yields, which in turn, decrease production costs.

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Rugged alternative to glass Dounce Tissue GrinderPrecision machined from No. 316 stainless steel Mortar and pestle provided as a matched setNot autoclavable

Wheaton Dura-Grind® Stainless Steel Dounce Tissue Grinders provide a rugged alternative to glass Dounce tissue grinders. Smooth pestle action requires less effort than glass tissue grinders. This unit is precision-machined of No. 316 stainless steel to exacting tolerance of .0005 in. (0.013mm). The mortar has a flat bottom allowing it to stand upright. Single pestle and mortar are supplied as a matched set; they are not autoclavable. Weight: 2 lb (0.9 kg)


357572 7 35 x 114 171 1357574 15 35 x 114 171 1357576 40 44 x 114 171 1

Ideal for tissue homogenatesUse with power homogenizationParts interchangeable Teflon® PestleAutoclavable

These grinders are precisely constructed with completely interchangeable parts to allow easier cleaning or replacement of damaged mortars. They are designed for use in preparation of tissue homogenates and other similar applications. The Teflon® pestle is for soft tissue such as brain or liver. The stainless steel rod is 0.25” (6.3 mm) in diameter. The grinding chamber clearance is .004” to .006” (0.1 to 0.15 mm). This unit is autoclavable and can be used for power homogenization as with our overhead stirrer. However, we strongly recommend the use of safety-coated tissue grinders (see 358003 series) for added safety. This unit is manufactured from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements.


358029 2 11 x 45 203 2358034 5 13 x 66 219 2358039 10 16 x 74 219 2358044 15 19 x 84 219 2358049 30 24 x 118 266 2358054 55 30 x 130 266 2

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358028 2 mL Mortar Only 2358033 5 mL Mortar Only 2358038 10 mL Mortar Only 2358043 15 mL Mortar Only 2358048 30 mL Mortar Only 2358053 55 mL Mortar Only 2358026 2 mL Pestle Only 2358031 5 mL Pestle Only 2358036 10 mL Pestle Only 2358041 15 mL Pestle Only 2358046 30 mL Pestle Only 2358051 55 mL Pestle Only 2

Exterior plastic coated glass mortar provide greater safetyIdeal for tissue homogenatesMortar made from Wheaton 33 low extractable borosilicate glassPTFE pestle with stainless steel shaft can be used with power homogenizationAutoclavable

Wheaton Safe-Grind plastic coated tissue grinders afford an added measure of safety over uncoated glass tissue grinders. The heavy plastisol coating protects the mortar from becoming scratched or checked, and assures you of a sure grip. More importantly, the plastic coating creates a greater safety factor in case the mortar cracks or breaks during power homogenization. The transparent coating allows an unobstructed view of the homogenate during grinding. Wheaton Safe-Grind tissue grinders are fully autoclavable. Grinding chamber clearance is .004” to .006” (0.1 - 0.15 mm). Diameter of the stainless steel shaft is 0.25” (6.3 mm).


358003 2 11 x 45 203 2358005 5 13 x 66 219 2358007 10 16 x 74 219 2358009 15 19 x 84 219 2358011 30 24 x 118 266 2358013 55 30 x 130 266 2


358004 2 mL Mortar Only 2358006 5 mL Mortar Only 2358008 10 mL Mortar Only 2358010 15 mL Mortar Only 2358012 30 mL Mortar Only 2358014 55 mL Mortar Only 2358026 2 mL Pestle Only 2358031 5 mL Pestle Only 2358036 10 mL Pestle Only 2358041 15 mL Pestle Only 2358046 30 mL Pestle Only 2358051 55 mL Pestle Only 2

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For a power homogenizer, see the Wheaton Overhead Stirrer on page 47.

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Made from Wheaton 33 low extractable borosilicate glassMicro size for extremely precise workReservoir and pouring lip are incorporated into the designAutoclavable

Similar to the all-glass Potter-Elvehjem style, but micro size for extremely precise work, this unit is designed for delicate hand operation. Clearance between pestle and tube is .004” to .006” (0.1 to 0.15 mm). A reservoir and pouring lip are incorporated into the design. This grinder is made from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements.


357844 0.1 4 x 65 110 2


357841 0.1 Pestle only 2357843 0.1 Mortar only 2

with Radial Serrations Mortar made from Wheaton 33 low extractable borosilicate glassRadial serrated Teflon® pestleInterchangeable partsAutoclavable

This grinder has a serrated Teflon® pestle that disperses homogenate into the mortar cylinder more efficiently. These units are precisely constructed with completely interchangeable parts, allowing for easier replacement of parts so you don’t need to keep pieces matched during cleaning. Grinding chamber clearance is .004” to .006” (0.1 to 0.15 mm). The unit can be autoclaved. Depth of the ser-rations is .020” to .025”. Diameter of the stainless steel shaft is 0.25” (6.3 mm).


357969 2 11 x 45 203 2357974 5 13 x 66 219 2357979 10 16 x 74 219 2357984 15 19 x 84 219 2357989 30 24 x 118 266 2357994 55 30 x 130 266 2


358028 2 mL Mortar Only 2358033 5 mL Mortar Only 2358038 10 mL Mortar Only 2358043 15 mL Mortar Only 2358048 30 mL Mortar Only 2358053 55 mL Mortar Only 2357966 2 mL Serrated Pestle Only 2357971 5 mL Serrated Pestle Only 2357976 10 mL Serrated Pestle Only 2357981 15 mL Serrated Pestle Only 2357986 30 mL Serrated Pestle Only 2357991 55 mL Serrated Pestle Only 2

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Homogenizes connective tissue including heart, muscle and lung

Made from Wheaton 33 low extractable borosilicate glass

Interchangeable components takes the hassle out of matching parts

Autoclavable

These tapered tissue grinders have an improved design, which incorporates a longer tapered surface

on both the mortar and pestle. The conical surface allows initial size reduction followed by passage through the cylindrical section for the final homogenization step. Grinding efficiency is improved, and less time is required as compared to the Tenbroeck and Potter-Elvehjem designs. When the pestle is “bottomed” within the mortar, the clearance is a uniform .004” to .006” (0.1 to 0.15 mm).

The components are interchangeably ground, which allows easier replacement of parts; there is also no need to keep parts matched during cleaning. The ground glass surface on both the mortar and pestle are capable of homogeniz-ing connective tissue including heart, muscle and lung. This unit can also be used for homogenizing skin and plant tissue, and is made from Wheaton 33 low extractable borosilicate glass that conforms to ASTM Type I, Class A and USP Type I requirements.


358103 1 11 x 49 130 1358107 3 11 x 86 220 1358111 5 13 x 93 220 1358115 15 18 x 114 240 1


358102 1 mL Mortar Only 2358106 3 mL Mortar Only 2358110 5 mL Mortar Only 2358114 15 mL Mortar Only 2358101 1 mL Pestle Only 2358105 3 mL Pestle Only 2358109 5 mL Pestle Only 2358113 15 mL Pestle Only 2

Teflon pestle with stainless steel rod fits power homogenizer

Homogenizes soft tissue such as brainMortar made from Wheaton 33 low extractable

borosilicate glassInterchangeable components takes the hassle out of

matching partsAutoclavable

This unit is the same as the Wheaton 358103 series, but with a Teflon® pestle for soft tissues such as brain. The stainless steel rod is 0.25” (6.3 mm) in diameter and is long enough to fit into the chuck of the Wheaton Overhead Stirrer.


358133 1 11 x 49 130 1358137 3 11 x 86 220 1358141 5 13 x 93 220 1358145 15 18 x 114 240 1


358132 1 mL Mortar Only 2358136 3 mL Mortar Only 2358140 5 mL Mortar Only 2358144 15 mL Mortar Only 2358131 1 mL Pestle Only 2358135 3 mL Pestle Only 2358139 5 mL Pestle Only 2358143 15 mL Pestle Only 2

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Ideal for grinding liver, intestine and heart tissueMade from Wheaton 33 low extractable borosilicate glassHollow pestle can be packed with icePour lip on mortarAutoclavable

All-glass Tenbroeck tissue grinders come with precision-made, interchangeable pestles and tubes; no need to keep components matched. This also allows for easier replacement of damaged parts. The clearance between pestle and tube is .0035-.0065” (0.09-0.16 mm). While this grinder is designed for hand operation, it can be slow-speed motor-driven by inserting a rubber stopper (size 00) and metal rod into pestle. The hollow handle permits packing with ice. This unit also features an expanded reservoir and pouring lip. Designed for tissues such as liver, intestines and heart, it’s made from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements.


357421 1 11 x 48 140 2357422 2 11 x 50 140 2357424 7 16 x 82 190 2357426 15 22 x 94 250 2357428 40 32 x 140 320 2

Made from Wheaton 33 low extractable borosilicate glassComplete kit includes 7 micro tissue grindersReplacement parts available separatelyAutoclavable

A complete selection of Wheaton Micro Tissue Grinders is conveniently packed in a lightweight protective carrying case. The 0.5 mL micro tissue grinder with screw cap can be used for tissue grinding as well as for additional procedures. This particular tissue grinder chamber can be safely joined with another threaded-glass component, if needed, using the Wheaton Connection®. The Micro Tissue Grinder Kit is packed in a high-density polyethylene case with foam inserts.

Cat. No. Description Qty/Kit Qty/Case

358204 Complete Kit 1 1

357421 1 mL Tenbroeck Tissue Grinder 1 2357535 0.5 mL Tissue Grinder, G.P.I. 13-425 cap 1 2357538 1 mL Dounce Tissue Grinder 1 2357844 0.1 mL Tissue Grinder 1 2357848 0.2 mL Tissue Grinder 1 2358029 2 mL Potter-Elvehjem Tissue Grinder 1 2358133 1 mL Tapered Tissue Grinder 1 1

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Teflon® spacer aligns the pestle and reduces aerosoling of the product

Made from Wheaton 33 low extractable borosilicate glass

Autoclavable

This unit consists of a finely ground glass pestle and tube. A Teflon® spacer aligns the pestle and reduces aerosoling of the product. The mortar and pestle come as a matched set. The pestle is manufactured from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements.


357848 0.2 7 x 33 115 2

With Teflon® Pestle and Screw CapMade from Wheaton 33 low

extractable borosilicate glassAutoclavable

This unit features a screw thread neck for use with the Wheaton Connection®.

The 13-425 cap has an open top and a Teflon® faced silicone rubber liner. The clearance between pestle and mortar is 0.002-0.006” (0.05-0.15 mm). These grinders are manufactured from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements.


357535 0.5 13 x 37 115 2


357536 Extra Mortar and Cap 10357537 Extra Pestle 2

Disposable tissue grinder eliminates cross contaminationUseful for radioactive samplesSmall grinder area allows for high sample recoveryMade from Wheaton 33 low extractable borosilicate glassAutoclavable

The Disposable Micro Tissue Grinder allows for homogenized tissue micro samples without cross-contamination. This unit is especially useful for radioactive samples. No washing is necessary; just dispose of the tissue grinder after use. The small grinding area allows high sample recovery. This disposable grinder is made from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements.

Mortar Size: 10 x 44 mm Pestle Size: 7 mm sphere

Cat. No. Size (mL) ID x Length (mm) Overall Length (mm) Qty/Case

357860 0.75 8 x 44 88 25

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liner used for sealing volatile samples has cross slits for easier needle penetration. An optional liner is also available, Teflon-coated silicone septa, which plugs into each of the 96 vials for better sealing capabilities. The cover locks into place and has built-in O-rings, which provide a secondary sealing system.

The Wheaton Multi-Tier Microtiter Plate is available assembled with the base plate, vials, spacers, Teflon/Silicone slit liner and cover, or as indi-vidual components.

Wheaton microplates provide the advantages of glass without the cost!

Specifications:

Standard 8 x 12 format with an alpha-numeric ID gridGlass conical bottom vials made of Type I borosilicate glass0.5 mL, 9 x 17 mm - fits in base plate1.0 mL, 9 x 30 mm - requires spacer and base plate1.5 mL, 9 x 44 mm - requires 2 spacers and base plate

Advantages:

Use the same base plate for all vials and sample sizesOpen-bottom base plate provides even heating or cooling of vials when

immersedCover locks the liner in place and prevents vial pulloutBase plate does not float in water bathConical design of glass vials permits maximum sample accessSecure fit of vials in plate permits use with shakers or vortex mixers during

sample preparationTeflon®/silicone liner with cross slits permits easier needle penetrationGlass vials provide inert solvent-resistant environment for samples

Plates manufactured using ABS (Acrylonitrile Butadiene Styrene) have good chemical resistance against acids and bases. Includes base plate, vials, spacers, Teflon/Silicone slit liner and cover, or as individual components.


W845100 0.5 mL Microplate System - with Glass Conical Vials 1W845110 1.0 mL Microplate System - with Glass Conical Vials 1W845106 1.5 mL Microplate System - with Glass Conical Vials 1

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Allow for power homogenization or mixingSpeed control capable of RPM/torque

combinationsUse only with tissue grinders with stainless steel

rodsKeyless chuck accepts shaft size to 7.9 mmOne year warranty

The Wheaton Overhead Stirrer is a compact unit with solid state speed control capable of providing RPM/torque combinations for most laboratory opera-tions such as homogenizing or mixing. The Wheaton Overhead Stirrer should be attached only to tissue grinders with Teflon® pestles and with stainless steel rods. Unit and motor are mounted in a housing with epoxy finish and is provided with a 6” aluminum rod and clamp for attachment to support stand. Keyless chuck accepts shaft sizes to 5/16” (7.9 mm). (Sup-port stand not included.) Unit comes with a one year warranty.

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Wheaton microplates extend the uses of traditional microplates. Their unique combination of glass and plastic provide the benefits of each component while maintaining low cost. Wheaton microplate systems are useful for autosamplers and sample storage. The glass inserts are inert, and do not contain extractable materials. Wheaton microplates have sufficiently large volume inserts that perform as mother plates for high throughput screening applications. The microplates can be used for small fraction collection, great for collecting fractions in a format compatible to your auto injector, saving time and effort.

Analytical chemistry laboratories use microtiter plates for different experi-mental assays, including sorbent assays, high throughput screening assays, and combinatorial chemistry analyses. Our microtiter plate can have a fixed height or it can be multi-tiered for adjustable height to support different-sized vials. The plate can be built via spacers, to hold capacities of 0.5 mL, 1.0 mL and 1.5 mL, depending on the height limita-tions of the instrument. The plate can hold various length 9 mm diameter conical bottom or flat bottom glass vials.

Many of the assays performed in the analytical chemistry laboratories require heating or cooling of the vials to simulate in vitro conditions or for temperature sensitive experiments. The heating or cooling is usually done using liquids or gases. Our base plate has an open bottom so that the microtiter plate can be immersed in heating or cooling fluids allowing total fluid contact with the vials. This permits uniform transfer of temperature to and from the vials.

The cover and liners for the plate provide a seal for individual samples to prevent sample evaporation and cross contamination. The Teflon®/silicone

Specifications:

Speed Range: 300-5,000 RPM (Under load) Electrical Requirements: 120 VAC, 50/60 Hz, 50 watts Dimensions: 4-1/2” x 5” x 12” (11.5 x 13 x 30 cm) Weight: 6 lbs (2.7 kg)

Cat. No Description Qty/Case

903475 Complete Unit 1


905275 Swivel Blade Paddle for Stirring Liquids in Vessels with Side Entry Port or for Vessels with Narrow Openings. Shaft 1/4” x 12” (6.3 x 305 mm). Blade Measures 1/2” x 2” (12.7 x 50.8 mm). Stainless Steel 1905462 Extension Rod, 1/4” x 6” (6.3 x 153 mm). For Use with Extension Sleeve for Lengthening Paddles. Stainless Steel 1905465 Extension Rod, 1/4” x 12” (6.3 x 305 mm). For Use with Extension Sleeve for Lengthening Paddles. Stainless Steel 1905475 Extension Sleeve for 1/4” (6.3 mm) Diameter Rods, with Set Screws. For Use in Lengthening Paddles. Stainless Steel 1

48


Plates manufactured using Topas COC (Cyclic Olefin Copolymer) have excellent clarity and heat/chemical resistance. TOPAS COC Plates have excellent chemical resistance to aqueous acids and bases and to most polar solvents. The plates can be autoclaved and sterilized. Samples can be heated in Topas COC Plates. Includes base plate, vials, spacers, Teflon/Silicone slit liner and cover, or as individual components.


W845102 0.5 mL Microplate System – with Glass Conical Vials 1W845112 1.0 mL Microplate System – with Glass Conical Vials 1W845108 1.5 mL Microplate System – with Glass Conical Vials 1W845101 0.5 mL Microplate System – with Flat Bottom Vials 1W845113 1.0 mL Microplate System – with Flat Bottom Vials 1W845107 1.5 mL Microplate System – with Flat Bottom Vials 1W845114 2.0 mL Microplate System – with Flat Bottom Vials 1

The Wheaton Multi-Tier™ Microplate is available assembled with the base plate, vials, spacers, Teflon®/Silicone slit liner and cover, or as individual components.


ABS SectionsW845120 Cover for Multi-Tier™ Microplate 5W845121 Spacer for Multi-Tier™ Microplate 1W845122 Base Plate 1

Topas SectionsW845116 Cover for Multi-Tier™ Microplate 5W845117 Spacer for Multi-Tier™ Microplate 1W845118 Base Plate 1

The glass conical vials are manufactured from Type I borosilicate glass. The glass provides an inert solvent resistant environment for samples. The vials fit securely in our base plate.

The vial loader is a fast, convenient choice for loading our vials into the base plate. Just peel away the packaging and place an empty base plate on top of the vial loader. Invert the entire unit, and the plate is ready for use.


W845140 0.5 mL Glass Conical Vials in Vial Loader 96W845141 1.0 mL Glass Conical Vials in Vial Loader 96W845142 1.5 mL Glass Conical Vials in Vial Loader 96

Sealing system is designed for Wheaton’s microplates and is compatible with most 96-well plates with rounded well holes. Molded PTFE/Silicone has 96 plugs that lock into the vials and create an inert environment for the samples while preventing cross contamination. The molded liner is available prescored, which can be easily pierced by an autoinjector needle. The liners are made of Teflon® lined medical-grade silicone that conforms to USP Class VI requirements.


W845124 Molded PTFE/Silicone Liner for Standard 96-Well Plates 5

Sealing system is designed for Wheaton’s microplates and is compatible with most 96-well plates with rounded well holes. Molded PTFE (polytetrafluoroeth-ylene)/Silicone 8-plug strips and individual plugs allow for limited testing in the Multi-Tier Plate or can be used for specific sample identification. The strips and plugs are made of Teflon® lined medical-grade silicone that meets the requirements of the USP Class VI.


W845130 Molded PTFE/Silicone Strips - 8 Plugs 12W845131 Prescored Molded PTFE/Silicone Strips - 8 Plugs 12 W845132 Molded PTFE/Silicone Plug Singles 100W845133 Prescored Molded PTFE/Silicone Plug Singles 100W845115 Liner PTFE/Butyl/PTFE, Flat, Pre-scored 5W845123 Liner PTFE/Silicone/PTFE, Flat, Pre-scored 5W845125 Molded Liner TFE/Butyl/TFE, 96 Plug 5W845126 Molded Liner TFE/Silicone/TFE, 96 Position 5W845127 Molded Liner TFE/Butyl/TFE, 96 Plug 5


W845119 Solid Clear Cover 5

49


With Cleanout Port and CapsAutoclavable

These flasks are similar to Catalog Number 351054, but they have a lower sidearm, a side cleanout port, a 38-430 size top cap and a 20-415 size sidearm cap. The caps have white styrene-butadiene rubber liners.

Cat. No. Capacity (mL) Volume (mL) Sidearm Size (mm) Qty/Case

351454 300 200 14 x 140 1351456 300 200 19 x 140 1351486 500 250 19 x 140 1351514 1000 600 14 x 140 1351516 1000 600 19 x 140 1

Maximizes aeration for improved growthBreak resistant lipAutoclavable

Designed for use on either rotary or reciprocating shakers, these flasks are specially designed to maximize aeration for improved growth. They feature a break-resistant lip, which accommodates a cotton plug or a stopper, and a marking spot on the side of flask. These flasks are manufactured from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements. The four baffle flask features a dam at the base of the neck that reduces splashing.

Cat. No. Capacity (mL) Qty/Case

With Three Baffles353255 50 1353257 125 1353259 250 1353262 500 1353264 1000 1353266 2000 1

With Four Baffles354235 50 1354237 125 1354239 250 1354242 500 1354244 1000 1

••

•••

For use as slant or shake culturesAutoclavable

These standard round-bottom culture tubes are manufactured from 51 expansion borosilicate glass that conforms to ASTM Type I, Class B and USP Type I requirements. The tubes can be reused many times. The caps have a white 14B

styrene-butadiene rubber liner or a Teflon® faced 14B styrene-butadiene rubber liner. See the Cap and Closure Section for replacement caps.


With White 14B Rubber Lined Cap (Autoclavable)358606 11 16 x 100 15-415 144358607 14 16 x 125 15-415 144358610 27 20 x 150 18-415 144

With Teflon® Faced 14B Rubber Lined Caps (Autoclavable)358646 11 16 x 100 15-415 144358647 14 16 x 125 15-415 144358650 27 20 x 150 18-415 144

Ideal for media storage and cell cultureAutoclavable

This flask is designed for biological/media storage or culture. The flask is manufactured from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements.

Cat. No. Capacity (mL) Cap Size Qty/Case

635134 25 20-400 1635165 1000 38-430 1

For use with colorimeter or spectrophotometerScratch and striation free side tubeAutoclavable

These flasks allow growth and turbidity reading at selected intervals without sampling. Simply tilt flask to allow filling of the sidearm and insert sidearm into colorimeter or spectrophotometer. The side tube is scratch and striation free. Designed to operate on a platform shaker, the flask holds approximately 350 mL to the base of the sidearm. Material cannot enter the sidearm when on the shaker. The break-resistant lip accommodates a cotton plug or stopper. These flasks are manufactured from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements.

Cat. No. Capacity (mL) Sidearm Size (mm) Qty/Case

351054 500 14 x 130 1

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••

•••

Three baffle flask Four baffle flask

50


Fluted design improves mixing35 – 1500 mL size

These units have screw caps with white styrene-butadiene rubber liners that reduce contamination of contents. The fluting is designed to improve mixing. The flasks are made from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements.

Cat. No. Capacity (mL) Cap Size Flask (mL) Qty/Case

355392 35 24-430 50 1355393 75 33-430 125 1355394 150 38-430 250 1355395 300 38-430 500 1355397 700 51-400 1000 1355398 1500 51-400 2000 1

Cat. No. Capacity (mL) Cap Size Graduations (mL) Flask (mL) Qty/Case

355442 35 24-430 Every 5 50 1355443 75 33-430 10, 25, 50, 75 125 1355444 150 38-430 50, 75, 100, 150 250 1355445 300 38-430 80, 100, 150, 300 500 1355447 700 51-400 Every 100 1000 1355448 1500 51-400 Every 150 2000 1

••

With Pourout and CapsFluted design improves mixingDeep skirted screw caps35 – 1500 mL size

These flasks feature deep-fluted sides to promote mixing. A dam below the sidearm retains untrypsinized tissue when decanting. A deep skirted screw cap on the sidearm prevents contamination of flask contents. The sidearm allows easier decanting of contents while retaining large particles behind the dam. Sidearm caps are 20-415 size. Caps have white styrene-butadiene rubber liners. Flasks are made from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements.

Cat. No. Capacity (mL) Top Screw Cap Size Flask (mL) Qty/Case

355752 35 24-430 50 1355753 75 33-430 125 1355754 150 38-430 250 1355755 300 38-430 500 1355757 700 51-400 1000 1355758 1500 51-400 2000 1

Cat. No. Capacity (mL) Top Screw Cap Size Graduations (mL) Flask (mL) Qty/Case

355802 35 24-430 Every 5 50 1355803 75 33-430 10, 25, 50, 75 125 1355804 150 38-430 50, 75, 100, 150 250 1355805 300 38-430 80, 100, 150, 300 500 1355807 700 51-400 Every 100 1000 1355808 1500 51-400 Every 150 2000 1

••••

For Hybridization Bottles see page 11.

Wheaton trypsinizing flasks are used to further reduce tissue to cellular components. Place tissue in flask, add trypsin and stir the vessel using the Wheaton Micro-Stir® magnetic stirrer. The deep-fluted flask design promotes mixing turbulence; a dam below the sidearm retains untrypsinized tissue when decanting. Dissociated cells can then be collected. Trypsinizing flasks are also useful when removing cells from microcarrier beads. Single cells can then be used to start a seed culture in a flask or multiple flasks. After reaching a specific density, cultures can seed a suspension culture flask or roller bottle for high volume cell production.

51


Wheaton Science Products has long been an innovator in cell culture flasks. The Wheaton Magna Flex® impeller produces high-yield cultures by maintaining even circulation while keep-ing cultures in suspension. Slow acceleration and slow stop from the Wheaton Micro-Stir® stirrer prevent cell detachment from microcarriers.

Wheaton also invented an adjustable impeller flask that does not have a rod extending through the top of a flask. Celstir® flasks feature adjustable blade impeller for increased aeration and dimpled bottom to prevent the accumulation of cells under the impeller.*

When used with the Micro-Stir, which can operate at very low

MantaRay® pictured with Micro-Stir® which is sold separately.

Single use spinner flask with integrated magnetPre-sterilized making it ready to use out of the boxBreak resistant unit has less potential for culture/sample lossOne MantaRay generates the same yield as 77 T175 T-flasks conserving on valuable incubator space and reducing costs associated with laboratory consumables

The MantaRay’s integrated paddle spinner system is optimized for culturing volumes of 500 mL through 1000 mL. Using a vertically oriented stirrer, the MantaRay ensures that cells are evenly distributed throughout the entire volume of culture.

Unlike conventional spinner flasks, the MantaRay’s paddle system comes ready-to-use, eliminating the need for assembly and disassembly of the device. The unique design of the spinner allows for even distribution of nutrients and oxygen; thus creating uniform media conditions for cell propagation. The constant agitation caused by the paddle system also prevents cells from settling to the bottom and adhering to the side walls.

The paddle is integrated with the top plate, which stabilizes the fixed stirrer height thus eliminating validation concerns due to height adjustments. The paddle and bag are heat sealed to the top plate reducing potential contamination related to adhesive compounds. The only required pieces of equipment for use of the MantaRay system are the MantaRay and a magnetic stirrer.

Use the MantaRay for growing mammalian cell cultures, as well as plant and insect cells. The MantaRay’s proprietary stirrer technology is designed to minimize damage to fragile cells, such as mammalian. The MantaRay also allows media and gases to be added or changed during the experiment under sterile conditions. The MantaRay can culture cells on micro-carrier beads, which the cells use as a growth surface. The MantaRay comes pre-sterilized. Simply fill the flask with media, add cells and let them grow. Use the MantaRay with the Wheaton Micro-Stir® slow speed (5-200 RPM) magnetic stirrer. See page 53 for details.

Cat. No. Description Working Volume (mL) Qty/Case

W356805-1 MantaRay 1000 1W356805-4 MantaRay 1000 4

••••

Impeller rod does not protrude up through top cap Sidearms for 500 mL - 8 L flask allow vented caps which enables the flask

to “breathe” while protecting from contamination.1:1 headspace for good gas exchangeNo contamination from stirring rodCompletely autoclavable

The Celstir Spinner Flask features an adjustable paddle blade impeller for better mixing. The addition of the bottom dimple to flasks 125 mL and larger improves circulation and reduces the accumulation of cells in the center of the flask. Celstirs provide maximum surface interface between culture and flask atmosphere. Use the Celstirs for microcarrier and suspension cultures such as insect cells, hybridomas, and adapted cell lines. The entire unit may be autoclaved at 121°C for 20 minutes. Celstirs are made from Wheaton 33 low extractable borosilicate glass that conforms to ASTM Type I, Class A and USP Type I requirements. Wheaton also offers a non-breakable stainless steel shaft assembly kit as a retrofit to your Celstir (see 356874 series). Units 500 mL and larger can be used with the Vented Cap (Cat. No. W240751).

Graduations: Sizes 125 to 1000 mL are graduated in 50 mL increments; sizes 3000 to 8000 mL are graduated in 500 mL increments; sizes 25 mL and 50 mL are non-graduated.

Cat. No. Volume (mL) Dia x H (mm) Cap Size, Top Sidearm Size Qty/Case

356873 25 38 x 122 38-430 15-415 1356875 50 38 x 141 38-430 15-415 1356876 125 65 x 155 51-400 33-430 1356879 250 85 x 175 51-400 33-430 1356882 500 110 x 190 100-400 45 mm 1356884 1000 130 x 250 100-400 45 mm 1356887 3000 178 x 341 100-400 45 mm 1356889 6000 258 x 404 100-400 45 mm 1356890 8000 293 x 445 100-400 45 mm 1

••

•••

speeds, the Celstir flask provides an optimum environment for most suspension lines, including insect cell cultures. Hybridomas and adapted cell lines can also be grown in these spinner flasks. Sidearms on Magna Flex and Celstir vessels 500 mL and larger were widened to accommodate monitoring and control devices or media inlet/outlet lines.

Wheaton has now moved cell culture another step forward with its innovative patented MantaRay® single-use culture flask. The MantaRay comes pre-sterilized to protect cell cultures. The MantaRay has a unique impeller that provides efficient, gentle stirring for both suspension or microcarrier culture. The MantaRay is disposable; discard it after use and save the time, trouble and expense of autoclaving and cleaning. *Dimpled bottom on 125 mL size and larger.

For sidearm replacement caps see page 41.

For vented caps see page 42.

52


Water jacket allows for precise temperature control Manufactured from Wheaton 33 low extractable borosilicate glassProportioned to provide a head space ratio of 1:1 or greaterThe impeller does not protrude through the top capFully autoclavablePTFE faced rubber-lined caps on sidearms

The Double-Sidearm Celstir with water jacket allows precise temperature control of its contents when operated with a recirculating water bath. The hose connectors accept 1/4 in (6.35 mm) ID tubing. This unit is made from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements. The 500 and 1000 mL sizes can also be used with Wheaton Vented Cap Cat. No. W240751.


356943 25 54 x 134 38-430 15-415 1356945 50 54 x 147 38-430 15-415 1356946 125 78 x 162 51-400 33-430 1356949 250 100 x 182 51-400 33-430 1356952 500 130 x 195 100-400 45 mm 1356954 1000 150 x 260 100-400 45 mm 1

Designed for use with microcarrier culturesSide arms for 500 mL - 6 L flasks allow vented caps which enables the

flask to “breath” while protecting from contaminationGentle, even stirring action

The Wheaton Magna Flex Spinner Flasks feature a flex-type, bulb-shaped glass impeller that rotates from a fixed position above liquid level around an indentation in the base of the flask. Primarily designed for use with microcarrier cultures, this unique stirring system increases stirring efficiency and provides a gentle, even circulation throughout the flask while keeping the beads in suspension.

All flasks have been proportioned to provide a head space ratio of 1:1 or greater. A removable stainless steel pin immobilizes the impeller during handling or decanting to prevent damage to cells or microcarriers. Sizes 500 mL and larger can accommodate the Wheaton Vented Cap (Cat. No. W240751).


356830 125 65 x 155 51-400 33-430 1356831 250 85 x 175 51-400 33-430 1356832 500 110 x 190 100-400 45 mm 1356834 1000 130 x 250 100-400 45 mm 1356837 3000 178 x 341 100-400 45 mm 1356839 6000 258 x 404 100-400 45 mm 1

••••••

••

•

Replacement for Double Sidearm Celstir and Jacketed Double Sidearm Celstir. This non-breakable stainless steel shaft, for replacing the glass shaft included with Celstir® flasks, may be retrofitted to the existing Celstir flasks.

Cat. No. For Flask Size (mL) Qty/Case

356874 25 1356877 50 1356878 125 1356880 250 1356883 500 1356885 1000 1356886 3000 1356888 6000 1356891 8000 1

Replacement for Double Sidearm Celstir and Jacketed Double Sidearm Celstir. This unit includes a top cap with liners, glass rod, magnet holder, magnet and stirring paddle (for sizes 125 mL or larger).


356893 25 1356895 50 1356896 125 1356899 250 1356902 500 1356904 1000 1356907 3000 1356909 6000 1356910 8000 1

Replacement for Magna Flex® Spinner Flasks. This unit includes a top cap with liners, glass stirring ball and connecting silicone tubing. This glass impeller assembly is designed for replacement on all Magna Flex® style flasks.


356841 125 1356842 250 1356843 500 1356844 1000 1356847 3000 1356848 6000 1356849 8000 1


Celstir Only356913 25 mL Celstir Flask Only 1356915 50 mL Celstir Flask Only 1356930 8000 mL Celstir Flask Only 1

Celstir & Magna-Flex356916 125 mL Celstir/Magna Flex Flask Only 1356919 250 mL Celstir/Magna Flex Flask Only 1356922 500 mL Celstir/Magna Flex Flask Only 1356924 1000 mL Celstir/Magna Flex Flask Only 1356927 3000 mL Celstir/Magna Flex Flask Only 1356929 6000 mL Celstir/Magna Flex Flask Only 1

Jacketed Celstir Flask356963 25 mL Jacketed Celstir Flask Only 1356965 50 mL Jacketed Celstir Flask Only 1356966 125 mL Jacketed Celstir Flask Only 1356969 250 mL Jacketed Celstir Flask Only 1356972 500 mL Jacketed Celstir Flask Only 1356974 1000 mL Jacketed Celstir Flask Only 1

53


5-200 RPM

Designed specially for cell culture applications requiring exceptionally slow speeds, gentle mixing preventing shear and low heat transfer. May be used in an environment with CO

2 and non-condensing humidity, and with a temperature

range to 50°C. Packaged 1 unit per case.

Cat. No. Plug Style

Single Place Micro-Stir®

W900700-A 120 VAC, North America W900700-B 100 VAC, Japan W900700-C 230 VAC, Continental Europe W900700-D 230 VAC, United Kingdom W900700-F 240 VAC, Australia/China W900700-G 230 VAC, Italy/Chile W900700-J 230 VAC, India

Four Place Micro-Stir®


150-1200 RPM

Designed specifically for use with suspension culture flasks. The effortless motor provides uniform speeds for great results with cell culture or small fermentations. An easy-to-read display allows exact RPM to be set. The smooth-running motor reduces heat transfer to medium being stirred. Takes flask sizes to 3 Liters. May be used in an environment with CO2

and non-condensing humidity, and with a temperature range to 50°C. Packaged 1 unit per case.

Cat. No. Plug Style

Single Place BioStir®


Four Place BioStir®


For BioStir® and Micro-Stir® Magnetic Stirrers

The Wheaton Remote Control allows all stirrer functions to be controlled and monitored without opening an incubator and disturbing the incubator contents. The remote control comes with a 12 ft. (3.66 m) cord which allows the stirrer to be placed in an incubator (such as the Wheaton roll-in or CO2

Incubators) while allowing freedom of placement for the remote controller.


W900704 Remote Control 1

•

•

•

Single Place BioStir

Four Place Micro-Stir

Computerized stirrer controlRotation speed clearly visible for consistent resultsVariety of operating modes for suspension or microcarrier cultures

Constant Speed Mode - Soft start to a constant stirring speed, this mode is used for suspension cultures.

Interval Start Mode - This mode turns the stirrer on and off using the soft start and stop for a set number of cycles, after which the stirrer runs at a constant speed that may differ from the speed used to attach the cells. This mode is useful for attaching cells to micro-carrier beads. There is no need to watch the culture and turn on the stirrer for a constant stir mode after the cells have attached.

2-Speed Stirring Mode - The stirrer slowly starts to a set speed, which is held for a user-defined period. The stirrer then softly changes to a final constant speed. This mode is also useful for microcarrier applications.

2 year warrantyConforms to UL, CSA, and CE StandardsOptional remote control

Specifications: Electrical: Operating voltage: .....................................................100-240 VAC, 50/60 Hz Power consumption: ........................................................................ 15 Watts Pollution Degree: ............................................................................... Class 2Environmental: Operating temperature: ............................................................. 15°C to 40°C Humidity: .........................................................80% up to 31°C, 50% at 40°C Altitude limit: ............................................................................. 2000 metersOperational: Operating speed: Micro-Stir: ........................................................................ 5 - 200 RPM BioStir: ....................................................................... 150 - 1200 RPM Maximum flask size: .........................................................................3 L flaskDimensions: H x W x D Single Place: .................................... 3.6 x 8.0 x 9.8 in (9.2 x 20.3 x 24.9 cm) Four Place: ..................................4.0 x 15.5 x 17.5 in (10.2 x 39.4 x 44.5 cm)Weight: Single Place: ........................................................................... 4.5 lb (1.9 kg) Four Place: .............................................................................. 10 lb (4.5 kg)

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54


Key areas of use are:Small culture bioreactorCell culture process optimizationInsect cell culture techniquesHybridoma cell culture methodsViral infection studies

The Cell Optimizer System® is a complete, small-volume cell culture bioreactor. This system provides a quick and accurate determination of optimum environmental cell growth parameters. Cell growth in the Cell Optimizer System takes place in a 250 mL, 500 mL, 1 liter or 3 liter working volume spinner flask with four (4) sidearms.

The system includes a Control Tower™, Control Tower Support Unit and Micro-Stir® magnetic stirrer. The Control Tower and support unit automatically controls pH via base addition and CO

2 gas, and dissolved oxygen utilizing air, O

2

and N2 gases.

Temperature is maintained by the use of a heating pad that eliminates the need for an incubator. Stirring speed (5-200 RPM) is achieved by manually setting the Micro-Stir® magnetic stirrer. Foam and nutrient feed control are available as options. The support unit includes a combination heater/pump module for temperature control and liquid base addition. A four-gas mixing module provides controlled additions of necessary gases to the vessel head space (and/or optional sparger). One year warranty.

For more specifics on the Control Tower see page 56.

Probe Specifications:

Temperature: ............................................................................100 Ohm RTD Dissolved Oxygen: ........................................................ Polarographic, 12 mm pH: ................................................................... Pressurized, Gel Filled, 12 mm

Units come configured for temperature, pH and dissolved oxygen. Other configurations are available upon request. For ease of record keeping and supervisory control, up to 32 Cell Optimizer System setups can be networked to the optional BIOPRO® PC supervisory software. See page 57 for details.

•••••

Cat. No. Plug Style Voltage Qty/Case

250 mL Cell Optimizer System®

W350000-A North America 120 VAC 1W350000-B Japan 100 VAC 1W350000-C Continental Europe 230 VAC 1W350000-D United Kingdom 230 VAC 1W350000-F Australia/China 240 VAC 1W350000-G Italy/Chile 230 VAC 1W350000-J India 230 VAC 1

500 mL Cell Optimizer System®


1 Liter Cell Optimizer System®


3 Liter Cell Optimizer System®


For more information on plug styles see page 188.

Cat. No. Description Voltage Qty/Case

WI055902 250 mL Spinner Flask with 2 Sidearms 1356922 500 mL Spinner Flask with 2 Sidearms 1WL054377 1 L Spinner Flask with 4 Sidearms 1WL054376 3 L Spinner Flask with 4 Sidearms 1

This 125 watt heating pad plugs into the Support Unit and is controlled by the Control Tower™. Dimensions: 12.7 x 12.7 cm.


I057638 Heating pad 100-120 VAC, 50/60 Hz, 125 watts 1I057599 Heating pad 220-240 VAC, 50/60 Hz, 125 watts 1

The 316 L stainless steel sampler is designed to work with either the 1 or 3 liter flask. The sampler is supplied with a plug valve to help maintain sterility of the vessel during sampling.


I057501 Sampler with plug valve, 30.5 cm 1

The 316 L stainless steel tubular cold finger may be used with either the 1 or 3 liter flask.


I057459 Cold Finger 1

Due to specific country requirements, the Cell Optimizer is not available for sale in Canada and Europe.

55


Key areas of use are:Large volume cell culture bioreactor Scale cultures up to production volumeAntibody or virus productionPilot plant studiesEconomical mass cell productionProduction of proteins or other cell products

The Mini-Pilot Plant™ spinner flask system is a large-volume cell culture bioreactor designed to scale cultures up to production volumes of cells or products. The system includes a Control Tower™, Support Unit, Overdrive™, heating blanket, sparger, and a choice of a 10 liter (six sidearms), 19 liter (six sidearms) or 45 liter (seven sidearms) vessel. The Control Tower™ and Support Unit automatically control pH via base addition and CO

2 gas, and

dissolved oxygen utilizing air, O2 and N

2 gases. For more specifics on the Control

Tower see page 56.

Temperature is maintained by the use of a heating blanket that eliminates the need for an incubator. Stirring speed is achieved by manual setting of the Overdrive system. Foam and nutrient feed control are available as options. The Support Unit includes a combination heater/pump module for temperature control and liquid base addition. A four-gas mixing module provides controlled additions of necessary gases to the vessel headspace and sparger. One year warranty.

Probe Specifications:

Temperature: ...........................................................................100 Ohm RTD Dissolved Oxygen: ........................................................ Polarographic, 12 mm pH: ................................................................... Pressurized, Gel Filled, 12 mm

For ease of record keeping and supervisory control, up to 32 Mini Pilot System setups can be networked to the optional BIOPRO® PC supervisory software. See page 57 for details.


The 10 liter Mini-Pilot Plant™ System comes with a Control Tower™ configured for temperature, pH and dissolved oxygen; Support Unit with a heater/pump module and a four-gas mixing module; Overdrive™ stirrer speed control (20-150 RPM); 10 liter vessel with six sidearms; temperature, pH and dissolved oxygen probes and cables; sparger; heating blanket; filters; and a 1 liter storage bottle (for base addition). This unit comes with one of seven standard electrical plugs. Additional configurations are available through special order.

Cat. No. Plug Style Voltage Qty


••••••

19 Liter Mini-Pilot Plant™ System with Control Tower™ configured for temperature, pH and dissolved oxygen; Support Unit with a heater/pump module and a four-gas mixing module; Overdrive™ stirrer speed control (20-150 RPM); 19 liter vessel with six sidearms; temperature, pH and dissolved oxygen probes and cables; sparger; heating blanket; filters; and a 1 liter storage bottle (for base addition). This unit comes with one of seven standard electrical plugs. Additional configurations are available through special order.



The 45 Liter Mini-Pilot Plant™ System with Control Tower™ is configured for temperature, pH and dissolved oxygen; a Support Unit with a heater/pump module and a four-gas mixing module; Overdrive™ stirrer speed control (20-150 RPM); 45 liter vessel with seven sidearms; temperature, pH and dissolved oxygen probes and cables; sparger; heating blanket; filters; and a 1 liter storage bottle (for base addition). This unit comes with one of seven standard electrical plugs. Additional configurations are available through special order.




The heating wrap plugs into the support unit and is controlled by the Control Tower™.


WI055974 Heating Wrap, 10 L 100-120 VAC, 700 watts 1WI055975 Heating Wrap, 10 L 220-240 VAC, 700 watts 1I057152 Heating Wrap, 19 L 100-120 VAC, 700 watts 1I057621 Heating Wrap, 19 L 220-240 VAC, 700 watts 1I057153 Heating Wrap, 45 L 100-120 VAC, 960 watts 1I057622 Heating Wrap, 45 L 220-240 VAC, 960 watts 1

The 316 L stainless steel sampler is designed to work with the 10, 19 or 45 liter Mini-Pilot Plant™. The sampler is supplied with a plug valve to help maintain sterility of the vessel during sampling.


I057502 Sampler with plug valve, 48.3 cm 1

The 316 L stainless steel tubular cold finger may be used with the 10, 19 or 45 liter Mini-Pilot Plant™.


I057617 Cold Finger 1

Due to specific country requirements, the Mini-Pilot Plant is not available for sale in Canada and Europe.

56


Monitor and control up to eight operation parametersMonitor and control parameters in multiple vesselsNetwork up to 32 Control Tower™ units

The Control Tower Digital Control System is a versatile and compact system for mammalian cell culture and insect cell culture. The system provides bench-scale monitoring and PID control of operation parameters. The Control Tower and Support Unit can be factory configured for any combination of the following parameters:

pHDissolved oxygenTemperatureAnti-foamNutrient feed

To further enhance the Control Tower, the BIOPRO® PC software (see page 57) provides sophisticated data acquisition, expanded process control capability and the ability to network together up to 32 systems. The Control Tower allows you to set and confirm setpoints and PID values, calibrate temperature, pH and DO sensors, and set times for feed pump operations. Monitor parameters and data entry by scrolling through the menus using the front panel keypad. All data is displayed on a two-line LCD. All cable connections for sensors and external devices (e.g., Support Unit) are on the rear of the unit.

Specifications:

Operating Voltage: ..................................... 100-120 VAC, 50/60 Hz, 10 watts 220-240 VAC, 50/60 Hz, 10 wattsDimensions: ............................................................... 16.5 x 22.8 x 38.7 cm Weight: ................................................................................. 4.5 kg (9.9 lbs)

Modular design allows for any combination of pumps, gas mixing and heating control The modu-lar support unit adds automated reagent pumping, gas mixing and heating capabilities to support the Control Tower™ in maintaining bioreactors. A single support unit can have up to four modules to con-serve bench space. The following modules can be combined in the factory to create the support unit:

Peristaltic pump module for automatic addition of liquid acid, base, antifoam or nutrients, with or without heater option

Four-gas mixing module for cell culture (air, O

2, CO

2, N

2 gas); flow rate 0-500 cc/min

Specifications:

Operating Voltage: 100-120 VAC, 50/60 Hz, 1000 watts maximum 220-240 VAC, 50/60 Hz, 1000 watts maximum Dimensions: 16.5 x 16.5 x 19.7 cm* *Base & 1 module; each additional module add 9.5 cm

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1 year warranty

This complete system comes with the vessel, impeller assembly and top drive motor. (When ordering, please specify 10, 19 or 45 liter vessels to provide working volumes of 8.5, 16 or 36 liters respectively). The large, adjustable height PTFE paddle (part of the impeller assembly) works together with the dimple in the bottom of the flask to provide gentle, even distribution and reduce dead areas. The impeller’s wetted compo-nents include 316 L stainless steel, virgin PTFE and Rulon® 641. Rulon 641 is a specially formulated compound of virgin PTFE and fillers approved by the FDA for use as a food component and thus for direct contact with food and drugs intended for oral consumption. The vessel is equipped with six or seven 45 mm sidearms to accommodate probes for monitoring and control, and tubing for sparging or media addition. The vessel and impeller sections are autoclavable; the lift-off top drive section is not autoclavable. This unit comes with one of seven standard electrical plugs. Additional configurations are available through special order.

Electrical Requirements: ...................................100-120 VAC, 60Hz, 30 watts 220-240 VAC, 50/60 Hz, 30 watts

Dimensions D x H (Vessel and Overdrive™ together): 10 Liter System: ............................................................... 23 cm x 64 cm 19 Liter System: ............................................................... 29 cm x 67 cm 45 Liter System: ............................................................... 41 cm x 78 cm Cat. No. Plug Style Voltage Qty/Case

10 Liter SystemW356700-A North America 120 VAC 1W356700-B Japan 100 VAC 1W356700-C Continental Europe 230 VAC 1W356700-D United Kingdom 230 VAC 1W356700-F Australia/China 240 VAC 1W356700-G Italy/Chile 230 VAC 1W356700-J India 230 VAC 1

19 Liter System356420-A North America 120 VAC 1356420-B Japan 100 VAC 1356426-C Continental Europe 230 VAC 1356426-D United Kingdom 230 VAC 1356426-F Australia/China 240 VAC 1356426-G Italy/Chile 230 VAC 1W356426-J India 230 VAC 1

45 Liter System356422-A North America 120 VAC 1356422-B Japan 100 VAC 1356428-C Continental Europe 230 VAC 1356428-D United Kingdom 230 VAC 1356428-F Australia/China 240 VAC 1356428-G Italy/Chile 230 VAC 1W356428-J India 230 VAC 1

Top Drive Motor Only356424-A North America 120 VAC 1356424-B Japan 100 VAC 1356430-C Continental Europe 230 VAC 1356430-D United Kingdom 230 VAC 1356430-F Australia/China 240 VAC 1356430-G Italy/Chile 230 VAC 1W356430-J India 230 VAC 1


356433 Impeller Assembly, 45 L 1WL052594 19 L Vessel with 6 Sidearms without Caps 1356471 19 L Vessel with 6 Sidearms with Caps 1I050567 45 L Vessel with 7 Sidearms without Caps 1W356473 45 L Vessel with 7 Sidearms with Caps 1WL055973 10 L Vessel with 6 Sidearms 1

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The Control Tower Control System and Support Unit are integrated with the Cell Optimizer and Mini-Pilot Plant System; however, they are available separately upon request. Contact Wheaton for more details.

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The BIOPRO®PC software is designed to extend the operational capabilities of the Control Tower™ System and Support Unit. This Microsoft® Windows® based software provides both supervisory control and monitoring of key process parameters, along with data archiving functions.

BIOPRO® PC Software features:Enhanced programmable control and data acquisition capability

for the Control Tower local controllerNetworking of up to 32 Control Tower units for central supervisory

control and data acquisitionPermanent documentation for batch recordsWorks with Microsoft Windows NT, 2000 and XPContinuous graphic displayTrend graphingProcess sequencingPassword protectionMinimal operator training requirement

The software stores all measured parameters and records all changes made to the system. Alarm conditions that occur during a run are highlighted and flagged. Externally measured variables such as total cell counts, nutrient and metabolic levels, and off-gas analysis data can be manually entered into the log for documentation and data analysis.

A continuous graphic display of system data allows for ease of monitoring while a permanent record is created for use in process validation or batch records. The BIOPRO®PC software offers trend graphing with user-adjustable data acquisition rates,

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variable channels and multi-tasking capability. The computer can be used for other operations while monitoring and recording data. All data stored in the computer is compatible with Microsoft Excel® for further analysis.

Consistent batch-to-batch results can be achieved by directly downloading batch recipes from the software to the Control Tower.

The “timed events” function allows for automatic extension of up to 200 steps. For added security, two levels of password protection are available.

BIOPRO® PC Monitor and Control Software

Includes: RS-232 to RS-485 adapter box with power module and appropriate line cord. 100-120 VAC, 220-240 VAC 50/60 Hz

This unit comes with one of seven standard electrical plugs. Additional configurations are available through special order.




For see Wheaton Cryule® Cryogenic Vials on pages 140-142 and Vial Racks on page 149.

For see the Wheaton Glass Media Bottles on page 12 and page 30 for Plastic Media Bottles.

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Allows precise rotation speedSmall scale mixing and agitationSmall footprintConforms to UL, CSA, and CE Standards1 year warranty

Perfect for Wheaton 30 mL or larger serum bottles, 100-125 mL media bottles or 38 x 200 mm culture tubes for the growth and observation of various cell cultures such as, single chicken or rat embryos. The Wheaton Mini Bottle Roller Culture Apparatus accommodates bottles 38 to 60 mm in diameter, up to 240 mm long, with bottle speeds of 3 to 45 RPM (38 mm bottle) and 2 to 30 RPM (60 mm bottle). Additional decks available separately. See information on Deck Kit below.

Electrical Requirements: ............................100-240 VAC, 50/60 Hz, 14 watts Bottle Size Accepted: ..................................38-60 mm x 240 mm (maximum) Bottle Speed: 38 mm Diameter Bottle ................................................... 3 to 45 RPM 60 mm Diameter Bottle ................................................... 2 to 30 RPMDimensions: W x D x H .............12 1/4” x 12 3/4” x 7 1/8” (32 x 32.5 x 18 cm) Weight: ........................................................................................ 18 lb (8 kg) Maximum temperature for use: ........................................................... 40°C

Warning: Use in a CO2 environment may reduce motor brush life.


W348923-A North America 120 VAC 1 W348923-B Japan 100 VAC 1 W348923-C Continental Europe 230 VAC 1 W348923-D United Kingdom 230 VAC 1 W348923-F Australia/China 240 VAC 1 W348923-G Italy/Chile 230 VAC 1 W348923-J India 230 VAC 1


Mini Bottle Deck Kit can be added to Mini Bottle Roller Culture Apparatus to accommodate an additional four bottles per deck. Can add up to a maximum of 2 kits per unit. Weight: 9 lb (4.1 kg)


W348920-CH Deck Kit for Mini Bottle Bench-Top Roller Rack 1

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Pictured with Two Additional Deck Kits

Use with glass roller bottlesAccomodates larger roller bottlesSmall footprintConforms to UL, CSA, and CE Standards1 year warranty

This conventional-type roller culture rack is designed to roll vessels 108 to 121 mm in diameter and up to 290 mm long. The two outside rollers can be moved inward to accommodate bottles as small as 75 mm in diameter. Each deck can accommodate two vessels. Additional decks available separately. See information on Deck Kit below.

Electrical Requirements: .............................. 100-240 VAC, 50/60 Hz, 14 watts Bottle Size Accepted: ........................................... 108 to 121 mm (maximum) Bottle Speed: 110 mm Diameter Bottle ............................................. 0.1 to 3.8 RPM Dimensions: W x D x H ............ 12 1/4” x 12 3/4” x 7 1/8” (32 x 32.5 x 18 cm) Weight: ........................................................................................18 lb (8 kg) Maximum temperature for use: ............................................................. 40°C

Warning: Use in a CO2 environment may reduce motor brush life.


W348924-A North America 120VAC 1 W348924-B Japan 100 VAC 1 W348924-C Continental Europe 230 VAC 1 W348924-D United Kingdom 230 VAC 1 W348924-F Australia/China 240 VAC 1 W348924-G Italy/Chile 230 VAC 1 W348924-J India 230 VAC 1


Small Bottle Deck Kit can be added to Small Bottle Roller Culture Apparatus to accommodate an additional two bottles per deck. Can add up to a maximum of 2 kits per unit. Weight: 9 lb (4.1 kg)

Cat. No. Description Qty /Case

W348930-CH Deck Kit for Small Bottle Bench-Top Roller Rack 1

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Pictured with Two Additional Deck Kits

59


The Original High Volume Production Apparatus

The State of the Art High Volume Production Roller Apparatus

Superior Product Designed to Maximize Production Spacing

Cost Buster Modular Line R2P Roller SystemNumber of Bottle Positions

5 or 8 5 5 or 8

Distance Between Decks

Production Spacing 6” (15.2 cm)

Modular Spacing7 1/8” (18.1 cm)

6 in (15.24 cm) Production spaced (available in 5 or 8 positions per deck) 7 1/8 in (18.1 cm) Modular spaced (available in 5 positions per deck)

Number of Decks

11 1 to 9 (easily modified) 1 - 9 (7 1/8 in. (18.1 cm) Modular spaced-5 positions per deck)1 - 11 (6 in (15.24 cm) Production spaced-5 positions per deck)11 (6 in (15.24 cm) Production spaced-8 positions per deck)

Unit Height Fixed Adjustable based on number of decks

5 position models are adjustable based on number of decks 8 position model is fixed

Number of Bottles

52 (5 bottles/deck) 86 (8 bottles/deck 104 172

01 (min) 45 (max) 1 90

1 - 55 (for 5 positions/deck-production spaced) 1 - 45 (for 5 positions/deck-modular spaced)1 - 110 1 - 90

Rotation Alarm Optional Optional Standard

Battery Backup Optional Optional Optional

Control/Operating System

Not Available Not Available CART2 software

}}} with

1700 cm2

bottle

} with 850 cm2 bottle

} with 1700 cm2

bottlewith 850 cm2 bottle

}with 1700 cm2

bottlewith 850 cm2 bottle

Please note that Roller Culture Apparatus is made to order and not returnable.

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Protects cells during power outagesProvides a minimum of 24 hours of auxiliary powerCan be used to rotate vessels during transport to harvest area

Protects your cells and process! The Wheaton Battery Backup System is designed to be installed in either the Modular or Cost Buster Roller Culture Apparatus. The system monitors the AC power and automatically switches between battery power and AC power as needed. The system will provide 24 hours of auxiliary power to the motor. This system can be used to rotate the roller vessels during transport to and from the warm room or incubator. The system consists of two long-life sealed batteries, an automatic charger system, and an independent speed control which can be preset to maintain a bottle speed of approximately 25% of full speed dur-ing the lose of the primary voltage.

Charger Specifications:

Recharge time is 8 hours for discharged batteries, shorter for partially discharged batteries. Charger automatically monitors temperature and adjusts charge voltage for correct charging levels at elevated incubator temperatures.


Factory Installed (Must be ordered with Roller Culture Apparatus) 349017 Battery Backup System for Modular Cell & Cost Buster Roller Apparatus 1

Field Retrofittable Battery Backup SystemSame as above except packaged for field installation on Wheaton Modular and Cost Buster Production Roller Culture Apparatus which conforms to UL, CSA & CE standards.

349018 Battery Backup System for Modular Cell & Cost Buster Roller Apparatus 1

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Monitors drive systemAlarm sound if any failure is detectedAlarm is visual & audible

The Wheaton Rotation Alarm System is designed to monitor the drive system on the Modular and Cost Buster Production Roller Culture Apparatus. When the sys-tem detects a failure it alarms. The alarm is visual (flashing LEDs), audible (loud buzzer), and can be tied into an existing equipment monitoring system through the external jack. The alarm system is installed in the Roller Culture Apparatus.

Visual: ............................................................ 3 LED indicators flash on and off

Audible: ....................................................................................... Loud buzzer

Remote signal: ...................................................... 4 pin panel mount mic jack

Alarm outputs: ....... Pulsating +12VDC (25ma), Dry contact closure .5A at 24VDCCat. No. Description Qty/Case

Factory Installed (Must be ordered with Roller Culture Apparatus)349011 Rotation Alarm System Modular Cell Production Roller Apparatus 1349013 Rotation Alarm System for Cost Buster Production Roller Apparatus 1

Field Retrofittable Rotation Alarm SystemSame as above except packaged for field installation on Wheaton Modular & Cost Buster Production Roller Culture Apparatus which conforms to UL, CSA & CE standards.

349014 Rotation Alarm System for Modular Roller Culture Apparatus 1349016 Rotation Alarm system for Cost Buster Production Roller Culture Apparatus 1

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Maximum production capacity in a minimum amount of spaceDistance between decks is 6” (15.2 cm)Available in 52 and 86 position models Drive units mounted on top to provide easy access for maintenance

and better air flow Optional rotation alarm and battery backup systems availableGreaseless bearings prevent leaching and eliminate bearing seizuresSoft start feature minimizes cell disruptionConforms to UL, CSA, and CE Standards1 year warranty

The Wheaton Cost Buster High Volume Production Roller Apparatus provides the maximum amount of potential cell culture growth area avail-able in the smallest amount of space. This is accomplished by reducing the spacing between decks. Controls and drive units are located on top of the unit, providing easy access for maintenance. The open frame design allows better air flow, which minimizes top-to-bottom temperature gradients.

Wheaton Cost Busters can accommodate roller bottles from 110 to 121 mm in diameter and up to 550 mm in length. Each roller is driven by nonslip belts and pulleys for positive traction (especially important for use with lightweight plastic bottles).

The Cost Buster High Volume Production Roller Apparatus is supplied without roller bottles.

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52-Position Cost Buster349000-A North America 120 VAC 1349000-B Japan 100 VAC 1349005-C Continental Europe 230 VAC 1349005-D United Kingdom 230 VAC 1349005-F Australia/China 240 VAC 1349005-G Italy/Chile 230 VAC 1W349005-J India 230 VAC 1


86-Position Cost Buster 348990-A North America 120 VAC 1348990-B Japan 100 VAC 1348995-C Continental Europe 230 VAC 1348995-D United Kingdom 230 VAC 1348995-F Australia/China 240 VAC 1348995-G Italy/Chile 230 VAC 1W348995-J India 230 VAC 1

For Incubators for your Roller Culture Apparatus see page 64.

Offered with 7 standard electrical plug choices. Additional configurations are available through special order.

Specifications:Electrical: .......100 - 120VAC, 50/60Hz, 35 watts ........................220 - 240VAC, 50/60Hz, 35 watts

Bottle Speed: ....0.25 – 5.3 RPM (110 mm bottle)

Operating Temp.: ..............................15 to 40°C

Humidity: ..................................80% up to 31°C

Altitude Limit: ................................ 2000 meters

Dimensions W x D X H: 52-Position: ...............30.9 x 24.3 x 74.5 (in) 52-Position: ...........78.4 x 61.6 x 189.4 (cm)

86-Position: ...............46.7 x 24.3 x 74.6 (in) 86-Position: .........118.7 x 61.6 x 189.4 (cm)

Weight: 52-Position: ...................... 250 lb (113.4 kg) 86-Position: ...................... 350 lb (158.8 kg)


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1 five-position base unit and up to 8 additional five position decksDistance between decks 7 1/8” (18.1 cm)Provides up to 75,000 cm2 total surface area for cell growthGreaseless bearings prevent leaching and eliminate bearing seizuresOptional rotation alarm and battery backup systems availableAll-position drive is standard allowing the use of glass or plastic

roller bottlesConforms to UL, CSA and CE Standards1 year warranty

The Wheaton Modular Cell Production Roller Culture Apparatus allows flexibility for scale-up and production of monolayer cell cultures in standard roller bottles. It accepts bottles from 108 to 121 mm in diameter and up to 550 mm in length. The system consists of a base drive unit and five-position roller deck to which additional roller decks can be added. A powerful DC motor, with soft-start speed control, drives the bottles through a series of durable nonslip belts.

The all-position drive model is a positive traction drive system for lightweight plastic bottles in which each roller is individually driven. Also included is a locking speed control knob that prevents accidental change in rotation rates.

This unit is available with one of seven standard electrical plugs shown. Additional configurations are available through special ordering. Contact Wheaton for more information. The Modular Cell Production Apparatus is supplied without roller vessels.

Shipping Unit: One per case

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The 5-Position Deck allows you to add one 5-position deck to your Modular Cell Production system.

Specifications:

Weight: ............................................................................................. 21 lb (9.5 kg) Dimensions W x D x H: ............ 29 3/4 x 24 3/8 x 7 1/8 in (75.6 x 62.9 x 18.1 cm)


348969 5-Position Deck 1

Specifications:

Electrical: ........100 - 120VAC, 50/60Hz, 35 watts .....................220 - 240VAC, 50/60Hz, 35 watts

Bottle Speed: .....0.25 – 5.3 RPM (110 mm bottle)

Operating Temperature: ....................15 to 40°C

Humidity: ................................... 80% up to 31°C

Altitude Limit: ................................. 2000 meters

Dimensions W x D x H: Base with 1 Deck: .....29.75 x 24.375 x 13.5 (in) 75.7 x 63.0 x 34.3 (cm) Base with 9 Decks: ...29.75 x 24.375 x 70.5 (in) 75.6 x 63 x 179 (cm)Weight: Base with 1 Deck: ...................... 70 lb (31.8 kg) Base with 9 Decks: ................... 238 lb (108 kg) Additional Decks: ....................... 21 lb (19.5 kg)

Cat. No. Plug Style Voltage

Base with 1 Deck - 5 Bottle Positions348960-A North America 120 VAC348960-B Japan 100 VAC348970-C Continental Europe 230 VAC348970-D United Kingdom 230 VAC348970-F Australia/China 240 VAC348970-G Italy/Chile 230 VACW348970-J India 230 VAC

Base with 2 Decks - 10 Bottle Positions348961-A North America 120 VAC348961-B Japan 100 VAC348971-C Continental Europe 230 VAC348971-D United Kingdom 230 VAC348971-F Australia/China 240 VAC348971-G Italy/Chile 230 VACW348971-J India 230 VAC







Base with 4 Decks - 20 Bottle Positions348963-A North America 120 VAC348963-B Japan 100 VAC348973-C Continental Europe 230 VAC348973-D United Kingdom 230 VAC 348973-F Australia/China 240 VAC348973-G Italy/Chile 230 VACW348973-J India 230 VAC




For Glass Roller Bottles see page 15.

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Utilize from research to productionModular; add capacity and options as your requirements changeFrom 5 to 55 positions per unit (holds 10 to 110 of the 850 cm2 vessels)Displays accurate RPM by entering vessel diameterWide speed range brushless DC motor, 0.25 to 8.0 RPMProgrammable rotation direction, fixed or reversingProgrammable soft-start and stop10 program storage registersOptional temperature monitoring, one to four sensors per unitOptional battery backupCART2 ready! Optional software for monitoring controlling,

& recording up to 255 unitsMulti-language display prompts. Choice of English, French,

German, Italian, Portuguese, or SpanishConforms to UL, CSA and CE Laboratory Standards1 Year Warranty

The Wheaton R2P Roller Culture Apparatus is designed to allow for

the development and growth of cell culture from research to production quantities without having to change equipment and revalidate protocols. This is achieved by giving you more flexible

controls and the ability to easily add options at any time. All R2P Roller culture units share the same motor,

software, controls and controllers, which makes moving culture conditions between units fast and easy.

All-Position Drive SystemEach roller is driven to allow for either glass or lighter-weight plastic roller culture vessels to be used.

User-Friendly Control PanelThe large digital display and keypad allow for easy input and monitoring of the settings. The unit continually displays the bottle RPM setting and the actual bottle RPM. The display remains accurate with any size bottle (110 to 121 mm diameter) by entering the bottle diameter. The unit can be programmed to rotate in either direction, and can reverse direction automatically at the time interval entered (better for roller bottles fluted along the longitudinal axis). The programmable soft start and stop feature avoids premature cell detachment. The settings can be saved in one of the ten memory locations for easy recall later.

Built-in Monitoring and AlarmsThe unit continuously monitors both the rotational speed and the drive train (the motor and belts). If the speed falls out of the entered tolerance or if the unit detects a loss of rotation, the unit displays an alarm, and sounds a loud buzzer. These alarms can also be tied to an existing monitoring system through an external jack. Optional features can be added at any time, including CART

2 and battery backup.

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Specifications:

Electrical: .................100 - 240 VAC, 50/60Hz, 20 watts

Bottle Dimensions Dia/L: 110 to 121 mm / up to 550 mm

Bottle Speed: .............. 0.25 – 8.0 RPM (110 mm bottle)

Deck Spacing: inches (cm) All Position Drive R

2P .........................6 in (15.24 cm)

Modular R2P .................................7 1/8 in (18.1 cm)

Operating Temperature: .............................. 15 to 40°C

Humidity: ...................... 80% up to 31°C; 50% at 40°C

Altitude Limit: ...........................................2000 meters

Dimensions W x D x H: Base with 1 Deck: .................... 31 x 24.5 x 13.5 (in) 78.8 x 62.25 x 34.3 (cm) Base with 3 Decks: .................. 31 x 24.5 x 25.5 (in) 78.8 x 62.25 x 64.8 (cm) Base with 11 Decks: ................ 31 x 24.5 x 73.5 (in) 78.8 x 62.25 x 186.7 (cm) Extra Deck: ................................... 31 x 24.5 x 6 (in) 78.8 x 62.25 x 15.24 (cm)

Weight: Base with 1 Deck: …… .....................52 lb (23.6 kg) Base with 3 Decks: ...........................85 lb (38.6 kg) Base with 11 Decks: .......................217 lb (98.4 kg) Additional Deck: ................................16.5 lb (7.5 kg)

Built-in Drive Monitoring System Alarm Visual: ............... Flashing display with fault displayed Audible: ..........................Buzzer (with On/Off control) External output: ........... NO or NC (programmable) dry contact (0.5A@ 24 VDC) through RJ-45 jack on the control housing front panel.

6”(15.24 cm) Deck SpacingCat. No. Plug Style Voltage Qty/Case

Base with 1 Deck - 5 Bottle Positions W348880-A North America 120 VAC 1W348880-B Japan 100 VAC 1W348880-C Continental Europe 230 VAC 1W348880-D United Kingdom 230 VAC 1W348880-F Australia/China 240 VAC 1W348880-G Italy/Chile 230 VAC 1W348880-J India 230 VAC 1

Base with 3 Decks - 15 Bottle Positions W348881-A North America 120 VAC 1W348881-B Japan 100 VAC 1W348881-C Continental Europe 230 VAC 1W348881-D United Kingdom 230 VAC 1W348881-F Australia/China 240 VAC 1W348881-G Italy/Chile 230 VAC 1W348881-J India 230 VAC 1

Base with 11 Decks - 55 Bottle PositionsW348882-A North America 120 VAC 1W348882-B Japan 100 VAC 1W348882-C Continental Europe 230 VAC 1W348882-D United Kingdom 230 VAC 1W348882-F Australia/China 240 VAC 1W348882-G Italy/Chile 230 VAC 1W348882-J India 230 VAC 1

Additional Deck for R2P All Position Drive W348888 R

2P 5-Position Deck, Factory-Installed (must be ordered with unit) 1

W348889 R2P 5-Position Deck, for Customer Installation 1

7 1/8” (18.1 cm) Deck SpacingCat. No. Plug Style Voltage Qty/Case



Additional Deck for Modular R2PW348886 R

2P 5-Position Deck, Factory-Installed (must be ordered with unit) 1

W348887 R2P 5-Position Deck, for Customer Installation 1


See page 63 for the 88-Position R2P System and R2P Accessories.


63


Highest capacity roller rack88 all position drive1 year warranty

This unit features 6 in (15.24 cm) production spacing between the decks and holds 8 bottles/per deck. It will not fit in standard incubators, but it will pass through standard doorways for warm rooms.

Specifications:

Dimensions W x D x H: .....................47 x 24.5 x 73.5 in (119.4 x 62.25 x 189.2 cm) Weight: ........................................................................................ 360 lb (163.6 kg)


Base with 11 Decks - 88 Bottle Positions W349020-A North America 120 VAC 1W349020-B Japan 100 VAC 1W349020-C Continental Europe 230 VAC 1W349020-D United Kingdom 230 VAC 1W349020-F Australia/China 240 VAC 1W349020-G Italy/Chile 230 VAC 1W349020-J India 230 VAC 1

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Remotely monitors and controls up to 255 R2P unitsRecords all settings, changes, alarms, and communication lossPassword-protected user levels maintain securityCan be easily added to R2P at any timeWorks with Windows® based softwareComputer Requirements: IBM Pentium PC or compatibleConforms to UL, CSA and CE Laboratory Standards

The Wheaton CART2 System precisely controls rotating speeds of up to 255

separate roller units. Each unit is monitored for rotation, speed deviation and communication. This system provides the perfect solution for GMP or FDA requirements.

IBM Pentium PC or compatible32 MB of RAM, minimumMicrosoft Windows® 2000, XPOne free serial port10 MB of free hard drive space, minimum

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Network Interface is a single-line network adapter for up to 255 R2P units.



Protects cells during power outagesProvides a minimum of 18 hours of auxiliary powerCan be used to rotate vessels during transport to harvest area

The system monitors the AC power and automatically switches to battery if AC power fails. Provides auxiliary power to maintain complete functionality of the R

2P

unit for a minimum of 18 hours at full capacity. The audible alarm and display backlight will not function during battery power mode.


W348899 Customer Installed 1W348898 Factory Installed (Must be ordered with R2

P Roller Culture Apparatus) 1


W348891 Customer Installed 1W348890 Factory Installed (Must be ordered with R2

P Roller Culture Apparatus) 1

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CART2 Screen Shot

CART2 Screen Shot


64


Designed specifically for use with Wheaton Roller Culture ApparatusNo lifting ramps in standard incubator. CO2 incubator has light weight ramps.40 cubic feet (1.1 m3) of space, fits:

Production-Spaced R2P unit (6 in (15.24 cm) between decks) with up to 11 decks

Modular-Spaced R2P unit (7 1/8 in (18.1 cm) between decks) with up to 9 decks

52-Position Cost BusterIndependent over-temperature safety protectionViewing window in the doorFour internal electrical outlets for accessoriesCan also be used for suspension culturesAvailable as standard or CO2 incubatorConforms to UL, CSA, and CE Standards1 Year Warranty

A convenient, space-saving and economical alternative to a warm room for small production runs. Designed to hold full-size units, it can be used in combination with the optional shelves to accommodate spinner flasks, rocker tables, and other types of equipment. Double-walled construction provides for temperature stability and easy cleaning. Shelves are not supplied with incubator.

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Standard Incubator753680 North America 120 VAC 1W753684-C-E Continental Europe 230 VAC 1W753684-D-E United Kingdom 230 VAC 1W753684-F-E Australia/China 240 VAC 1W753684-G-E Italy/Chile 230 VAC 1W753684-J-E India 230 VAC 1

CO2 IncubatorI057606 North America 120 VAC 1WI057606-C-E Continental Europe 230 VAC 1WI057606-D-E United Kingdom 240 VAC 1WI057606-J-E India 230 VAC 1


Incubator Shelves (Not supplied with incubators)Cat. No. Description Qty/Case753685 Shelf for Standard Incubator 1WI056028 Shelf, Stainless Steel for CO

2 Incubator 1

Specifications: Specifications are for Standard & CO

2 Incubators unless specified otherwise

CONSTRUCTION:

External: ......................................................................................Painted Steel Internal: Standard ..................................................................................Painted Steel CO

2 ........................................................................................Stainless Steel

Door Weight: ................................................................................ 75 lb (34 kg)

Shelf Weight Maximum: .............................................................. 80 lb (36 kg)

Shelves Per Unit Maximum: .......................................12 Shelves (sold separately)Recommend not blocking perforated shelf surface by more than 60% to allow adequate air flow.)

Thermal Factor: ........................................................................................R13Accept standard cleaning chemicals

CO2 GAS: Gas shuts off when door is opened or switch is turned off

CO2 Connection: ........................................................1/4" (6.35 mm) hose barb CO2 Concentration Range: .....................0-20% adjustments in 0.1% increments CO2 Pressure to Indicator Inlet: ..................Rated @ 5-40 psi (0.345-2.76 bars) not to exceed 40 psi (2.76 bars) CO2 Recovery time When Door is Opened and Closed:

10 Second Opening ..................................................................Instantaneous 30 Second Opening ..................................................................... 10 minutes

ELECTRICAL / ELECTRONICS: Requirements: ......................................................... 120-240 VAC, 1500 watts Power Switch: .................................................................. On/Off rocker switch Temperature Keypad: ................................... Digital indicator & actuator keypad AC Receptacles: ...................... Four internal (customized for voltage differences) Heating Light Heaters: ..............................................................................One

Setting maintained if power is interrupted:

Circuit Breaker: ................................ None on CE Units (CE units include fuse) Failsafe Feature: Secondary mechanical high limit thermostat with indicator light

TEMPERATURE:

Heat: .................................. Forced-air circulation with digital temperature control Temperature Range: ............................... Ambient +8 to 70°C (empty incubator) Temperature Uniformity: .......................... to ±0.5°C @ 37°C (empty incubator) Sensitivity: Standard Incubator: ...........................................................................±0.1°C CO

2 Incubator: ...................................................................................... ±0.1°C

DIMENSIONS:

Capacity: ..............................................................................40 cubic feet (1.1 m3) Dimensions W x D x H: Interior: .........................................................35 x 26 x 76" (89 x 66 x 193 cm) Exterior: ................................................41 x 34 x 87" (104.2 x 86.4 x 221 cm) Weight: Net Weight: .............................................................................730 lb (331 kg) Shipping Weight: ......................................................................850 lb (386 kg)

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These color-coded clamps hold standard taper joints securely under moderate pressure. Molded from polyoxymethylene (POM), an acetal resin, the clamps will not scratch glass and can withstand temperatures up to 150°C. These clamps are resistant to bases and weak acids; they are not resistant to halogen gases and concentrated acids.

Cat. No Standard Taper Joint Size Color Qty/Case

297746 14 Yellow 12297749 19 Blue 12297752 24 Green 12297755 29 Red 12297757 Assorted Assorted 24

Inert contact surfaceEasy to assemble Leak-tight, positive connections

The Wheaton Connection® safely joins two exterior threaded glass components without hooks, springs or clamps. The screw cap configuration creates a grease-free, vacuum-tight seal. This connection can be used under moderate pressure to generate a gas or deliver a reagent under an inert atmosphere. It is available with white polypropylene or black phenolic caps. The Wheaton Connection® may be autoclaved and is used in the Wheaton Micro Kit® catalog number 774600 and Wheaton Semi-Micro Kit® catalog number 774650. The Wheaton Connections®

made from phenolic have a working temperature of up to 180°C.

Cat. No. Screw Thread Size Qty/Case

With White Polypropylene Cap125478 13-425 and 13-425 6125480 13-425 and 20-400 6125482 13-425 and 24-400 6125486 20-400 and 20-400 6125488 20-400 and 24-400 6125504 24-400 and 24-400 6

With Black Phenolic Cap125492 13-425 and 13-425 6125494 13-425 and 20-400 6W125495 13-425 and 20-400 6125496 13-425 and 24-400 6125497 20-400 and 20-400 6125499 20-400 and 24-400 6125500 24-400 and 24-400 6

•••Adapters & Connectors ..................................................... 65 - 66

Chemistry Glassware ........................................................ 67 - 69Chemistry Kits ................................................................. 70 - 72Desiccators ..................................................................... 73 - 74 Filtration Apparatus .......................................................... 75 - 80Solids Determination ............................................................... 82Weighing Dishes ..................................................................... 82

T his section consists of analytical apparatus that are commonly used in organic chemistry and the examination of environmental samples.

The majority of the glassware in this section is fabricated with either our exclusive Clear-Seal® joints or the Wheaton Connection® screw thread finish ends.

The smooth, unground Clear-Seal joints seal without grease, reducing the possibility of seizing. Clear-Seal joints are manufactured to American Standard Taper dimensions and can be used interchangeably with ground joints. However, when Clear-Seal joints are used exclusively, there is little chance of freezing and no chance of contamination or mess as with other sealing methods.

The Wheaton Connection basic design links two exterior-threaded glass components. The connection eliminates grease and the contamination

that is commonly associated with ground joints. Also eliminated are clamps, hooks and springs. Durable polypropylene caps, high temperature phenolic caps, and Teflon® faced silicone rings ensure a tight, inert seal. Lab safety is increased by completely preventing frozen joints.

•••••••

use Wheaton's Safety Coated Bottles when storing reagents and chemicals. See page 16.

66


These adapters enable interfacing between standard semi-micro glassware and micro systems, with a Clear-Seal inner joint on one end and a screw thread for the Wheaton Connection® on the other end. The 13-425 thread size comes with a screw cap and an “O” ring to seal thermometers, gas inlet tubes, etc. An optional thermometer adapter (Cat. No. 165996) is offered for the 20-400 size. The Clear-Seal Connecting Adapter is made with Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements.

Cat. No. Screw Thread Size Standard Taper Joint Qty/Case

139479 13-425 19/22 1139506 20-400 14/20 1139509 20-400 19/22 1139513 20-400 24/40 1

This unit features a Clear-Seal outer joint on one end and a screw thread for the Wheaton Connection® on the other end. It’s ideal for connection to rotary evaporators, V Vials®, one-dram vials, as well as many other types of vials. The joint is pictured here with the Wheaton Connection® and a V Vial® (both not included). It’s made with Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements.

Cat. No. Screw Thread Size Standard Taper Joint Qty/Case

139626 13-425 14/20 1139629 13-425 19/22 1139633 13-425 24/40 1139656 20-400 14/20 1139663 20-400 24/40 1

These joints are formed from a unique wall tubing that is heavier than standard wall tubing for extra strength. They’re manufactured from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements.

Cat. No. Standard Taper Joint OD (mm) Qty/Case

757766 14/20 12 6757769 19/22 17 6757770 19/38 17 6 757772 24/25 21 6757773 24/40 21 6757775 29/26 26 6757776 29/42 26 6

These joints are formed from a unique wall tubing that is heavier than standard wall for extra strength. They are manufactured from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements.

Cat. No. Standard Taper Joint OD (mm) Qty/Case

758966 14/20 18 6758969 19/22 22 6758970 19/38 22 6758972 24/25 28 6758973 24/40 28 6758976 29/42 33 6

This adapter has an “O” ring which holds a thermometer or gas inlet tube securely. A grease-free Wheaton Connection® on one end provides an inert connection with exterior-threaded glass components. This adapter can also be used with gas inlet tubing, etc., and will accommodate any tube with an OD of 5 to 7 mm.

Cat. No. Screw Thread Size Qty

165996 20-400 1

For BOD Bottles, see pages 3-4.

For Bottle Top Dispensers, see pages 99-100.

67


This apparatus is used for arsenic analysis using silver diethyldithiocarbamate (SDDC) colorimetric method described in the 21st Edition (2005) of Standard Methods for the Examination of Water and Wastewater, Method 3500-As B. This design eliminates the ball and socket joint connecting the scrubber and absorber components. The assembled apparatus is freestanding and easy to use. It is manufactured from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements.


180030 Arsine Generator, Complete 1

Component Parts180034 Scrubber Absorber Tube 1635965 Reaction Flask, 200 mL, 24/40 Joint 1357331 Short Disposable Pasteur Pipettes (146 mm long) 1000297746 Clamp, Acetal Resin, Size 14, Yellow 12297752 Clamp, Acetal Resin, Size 24, Green 12970440 Drying Tube, 14/20 Joint 1

This generator was engineered for arsine analysis using the silver diethyldithiocarbamate (SDDC) colorimetric method (meets ASTM/USP and EPA specifications). The arsine absorber (Cat. No. 180021) has a socket joint, while the scrubber tube (Cat. No. 180022) has a standard taper 24/40 Clear-Seal® joint and a spherical 12/2 ball joint held together by a size 12 stainless steel ball joint clamp. This unit is manufactured from Wheaton 33 low

extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements. Reference: “Standard Test Method for Arsenic in Water,” ASTM D2972-97.


180020 Arsine Generator, Complete 1

Component Parts180021 Arsine Absorber Joint 1180022 Arsine Scrubber Tube 24/40, 12/2 Joint 1635926 Erlenmeyer Flask, 125 mL, 24/40 1297784-12 Clamp, Size 12/2, Stainless Steel 1

This is a modified version of catalog number 180020 for arsine analysis eliminating the cumbersome ball and socket joint connecting the scrubber and absorber tubes which conforms to ASTM/USP and EPA specifications. The freestanding system has a standard taper 24/40 Clear-Seal® joint. This unit is manufactured from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements. Reference: “Standard Test Method for Arsenic in Water,” ASTM D2972-03.


180023 Arsine Generator, Modified, Complete 1

Component Parts180024 Scrubber Absorber Tube 1635926 Erlenmeyer Flask, 125 mL, 24/40 1

This unit is manufactured from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements. Use this kit for removing interfering substances in quantitative analysis for cyanide. The sample is reacted with H2

SO4 and MgCl

2, which converts any cyanide to HCN.

HCN distills over and is absorbed in a NaOH solution, which is then analyzed for CN. The unit utilizes standard taper Clear-Seal joints. Reference: “Standard Test Method for Cyanides in Water,” ASTM D2036-06.


377160 Cyanide Distillation Kit, Complete 1

Component Parts377161 Absorber Tube, 19/38 1377162 Absorber Top, 19/38 1377163 Cold Finger, 29/42 1377164 Cold Finger Jacket, 29/42 1377165 Flask, 1000 mL, 19/38 1377166 Inlet Tube, 19/38 1

Use the Wheaton TCLP Extraction Bottle to determine if any of 40 contaminants is in waste materials. See page 5 for ordering information.

68


This distillation kit, with sulfide removal, eliminates the interfering substances in quantitative analysis, therefore enabling compliance to procedures described by EPA 600/479/020, Method 335.2. The unit is manufactured from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements.


377260 Cyanide Distillation Kit, Complete 1

Component Parts709882 Scrubber, with 29/42 Joints 1709782 Absorber, with 29/42 Joints 1377163 Cold Finger, 29/42 1377164 Cold Finger Jacket, 29/42 1377165 Flask, 1000 mL, 19/38 1377166 Inlet Tube, 19/38 1

With Standard Taper Clear-Seal® Grease-Free Joints

Use this unit to generate diazomethane and other gases for gas chromatography, mass spectroscopy, and NMR studies using one millimole of Nitrosoguanidine (MNNG). It features Clear-Seal® grease-free joints that have never been ground, and doesn’t require an “O” ring. This generator comes complete with a plastic standard taper joint clamp, a 13-425 screw cap with cut-out top and a Teflon® faced rubber liner. They are manufactured from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements. Note: Please use a safety shield and efficient fume hood when generating diazomethane or similar gases.

Cat. No. Description Approx Height (mm) Qty/Case

281135 Diazomethane Generator, Complete 190 1W240593 13 mm Teflon® Faced Butadiene Septa — 100

•

Use this unit to generate diazomethane and other gases for gas chromatography, mass spectroscopy, and NMR studies using one millimole of nitrosoguanidine (MNNG). It comes complete with an “O” ring clamp, a 13-425 screw cap with a cut-out top and a Teflon® faced rubber liner. The generator is made from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements. Note: Please use a safety shield and efficient fume hood when generating diazomethane or similar gases.

Cat. No. Description Approx Height (mm) Qty/Case

281155 Diazomethane Generator, Complete 200 1281158 “O” ring for Cat. No. 281155 12W240593 13 mm Teflon® Faced Butadiene Septa 100

This evaporative concentrator is used for analyte concentration in organic solvents. The center flask is eliminated for better analyte retention. The unit consists of a three-chamber Snyder column, 10 mL receiving vessel, and an acetal standard taper clamp. It is manufactured from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements.


297942 10 mL Concentrator, Complete 1

Component Parts297800 10 mL Receiving Vessel 19/22 Clear-Seal® Joint (graduated 0-1 x 0.1 mL; 2-10 x 0.5 mL) 1297749 Clamp, Acetal Resin, Size 19, Blue 12

The small-scale Kuderna-Danish concentrator is engi-neered for analyte concentration in organic solvents prior to analysis. The Wheaton Connection® eliminates the need for hooks, springs, and ground joint clamps common to standard designs. The complete unit consists of 112 mm condenser, 40 mL flask, 2 mL receiving vessel, Wheaton Connection® 125478, and Wheaton Connection® 125486. All wetted parts are manufactured from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements or Teflon®.



Component Parts297771 Condenser, 112 mm, 20-400 Screw Thread 1297773 40 mL Flask, 13-425 and 20-400 Screw Threads 1W297774 2 mL Receiving Vessel, 13-425 Screw Thread 1125478 Wheaton Connection®, 13-425 to 13-425 6125486 Wheaton Connection®, 20-400 to 20-400 6

AccessoriesThe following item is not included in the complete kit, but can be purchased separately. W297772 Micro Snyder Column, 158 mm, 20-400 Screw Threads 1

281135 281155

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This unit is designed for the concentration of trace amounts of analytes in an organic solvent prior to analysis. Wheaton Connections® eliminate the hooks, springs and clamps common to standard ground joint designs. The complete unit consists of 215 mm three-ball Snyder column, 500 mL flask, 10 mL receiving vessel, and two Wheaton Connections®. All threads are 20-400. All wetted parts are manufactured from either Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements or Teflon®.


297810 Kuderna-Danish Concentrator, Complete 1

Component Parts297811 Snyder Column, 215 mm, Three-Ball, 20-400 1297815 Flask, 500 mL, 20-400 Top, 20-400 Bottom 1W297779 10 mL Receiving Vessel, with 20-400 Screw

Thread (Graduated 0-1 x 0.1 mL; 2-10 x 0.5 mL) 1125486 Wheaton Connection®, 20-400 to 20-400 6

AccessoriesThe following items are not included in the complete kit, but can be purchased separately 297814 Flask, 250 mL, 20-400 Top, 20-400 Bottom 1297816 Flask, 1000 mL, 20-400 Top, 20-400 Bottom 1

This unit is designed for the concentration of trace amounts of analyte in an organic solvent prior to analysis. The smooth Clear-Seal® joints eliminate the need for grease, reducing the possibility of contaminating the sample. The complete unit consists of 270 mm three-ball Snyder column, 500 mL flask, and 10 mL receiving vessel. This concentrator is manufactured from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements.


297883 Kuderna-Danish Concentrator, Complete 1

Component Parts384353 Snyder Column, 270 mm, Three-Ball, with 24/40 Clear-Seal® Joints 1297825 500 mL Flask, with 24/40 Top and 19/22 Bottom Clear-Seal® Joints 1297800 10 mL Receiving Vessel, with 19/22 Clear-Seal® Joint

(Graduated 0-1 x 0.1 mL; 2-10 x 0.5 mL) 1

Use this apparatus for continuous extraction of analytes from a solid into an organic solvent. The Wheaton Connection eliminates the need for hooks, springs and clamps common to standard ground joint designs. As the flask containing the solvent is heated, vapors rise in the larger outside tube, enter the water-cooled condenser, and liquate. When the liquid level in the extractor reaches the top of the bent tube, siphoning action returns the extract-enriched solvent to the flask. All wetted parts are manufactured from either Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements or Teflon®.


415500 Micro Soxhlet Extractor, Complete 1

Component Parts415501 85 mm Condenser, 24-400 Screw Thread on Bottom and 13-425 Screw Thread on Side 1415502 Extractor, 24-400 Screw Thread on Top and 20-400 Screw Thread on Bottom 1125486 Wheaton Connection® 20-400 to 20-400 6125504 Wheaton Connection® 24-400 to 24-400 6635134 25 mL Erlenmeyer Flask, 20-400 Screw Thread 1962515 Hose Connector, 13-425 6W416788 Extraction Thimble, Glass, 15 x 60 mm, 170-220 μm Porosity 1

Use this apparatus for continuous extraction of analytes from a solid into an organic solvent. As the flask containing the solvent is heated, vapors rise in the larger outside tube, enter the water-cooled condenser, and liquate. When the liquid level in the extractor reaches the top of the bent tube, siphoning action returns the extract-enriched solvent to the flask. All wetted parts are manufactured from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements.


416000 125 mL Soxhlet Extractor, Complete 1416005 250 mL Soxhlet Extractor, Complete 1416010 500 mL Soxhlet Extractor, Complete 1

Component Parts415000 190 mm Extractor with 24/40 top inner joint and 34/45 bottom outer joint 1415005 205 mm Extractor with 24/40 top inner joint and 45/50 bottom outer joint 1 415010 250 mm Extractor with 24/40 top inner joint and 55/50 bottom outer joint 1334003 Allihn Reflux Condenser, 34/45 1334014 Allihn Reflux Condenser, 45/50 1334025 Allihn Reflux Condenser, 55/50 1428126 Flat-Bottom Flask, 125 mL, 24/40 1428146 Flat-Bottom Flask, 250 mL, 24/40 1428164 Flat-Bottom Flask, 500 mL, 24/40 1

Give us a call if you need technical assistance1.800.225.1437 (U.S. & Canada Only) I +1.856.825.1100

Business hours: 8:00 AM to 5:00 PM EST in U.S.A.

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W heaton Science Products innovative microscale kits provide many advantages over traditional macroscale glassware. The reduced quantity of chemicals required for experiments translates into

less chemical waste disposal. Savings on waste disposal are realized by many universities, colleges and industrial laboratories.

Exposure to toxic materials and their vapors have been virtually eliminated with the use of microscale glassware. Less solvent consumption affords better air quality and reduced risk of fire or explosion. Microscale users enjoy improved skills in chemistry with quicker setup times and shorter reaction times, allowing more time for experiments. The Wheaton Connection®, a threaded connector joining two glass pieces, eliminates the use of grease or joint clamps. The Wheaton Connection® is available with white polypropylene or black phenolic caps. The Wheaton Connection® allows the use of inexpensive Wheaton vials as single-use reaction flasks, greatly reducing cleaning requirements. The vials are also used for the storage of intermediate compounds or final reaction products.

With Wheaton Kits, you get apparatus for a wide range of organic chemistry experiments at a lower price than if you purchase the components separately. Your purchasing department and stockroom will appreciate the convenience of ordering and issuing complete kits.

Wheaton standard taper Clear-Seal® grease-free joints provide smooth, unground surfaces, which seal without grease and reduce seizing and contamination from grease. Clear-Seal joints can replace ground units in any application; they also provide other important advantages over ground joints:

Superior in vacuum and radioactive applications. The smooth surfaces resist contamination. Cleaning is simple and thorough.

Interchangeable with ground joints. Transparent mating surfaces. All chemical changes and thermometer scales remain visible through the joints. Greater strength. Clear-Seal joints have never been weakened by grinding. In addition, Clear Seal joints are

formed from special wall tubing that is heavier than standard wall for extra strength. Manufactured from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and

ASTM E-438 Type I, Class A requirements.

Clear-Seal joints are produced to American Standard Taper dimensions and can be used interchangeably with any standard taper ground joints. Enjoy Clear-Seal® joints exclusively or couple them with ground joints and get maximum results. Wheaton Micro Kit catalog number 773900 is packed in a high-density polyethylene case with a foam insert to protect components against breakage.

Cat. No. Description Qty Included in Kit Replacement Part Qty/Case

773900 Complete Kit 1 —

Component and Replacement Parts109933 Claisen Adapter 1 1123753 Connecting Adapter 1 1139479 Thermometer Adapter with “O” ring, 13-425 Cap 1 1168353 Vacuum Distilling Adapter 1 1327342 West Condenser, 200 mm 1 1385772 Distilling Column, 200 mm 1 1WF034088 Round-Bottom Flask, 25 mL 1 1WF034093 Round-Bottom Flask, 50 mL 1 1W438174 Round-Bottom Flask, 100 mL 1 1438764 Round-Bottom Flask, 250 mL 1 1438782 Round-Bottom Flask, 500 mL 1 1690170 Separatory Funnel with Teflon® Stopcock, 125 mL 1 1923769 Teflon Stopper 1 1964453 Bleed Tube 1 12

High density polyethylene cases and foam inserts. Sold separately.


774510 Case for Micro Kit Cat. No. 773900 6774515 Foam insert for Micro Kit Cat. No. 773900 6

•

•••

•

71


Wheaton is pleased to offer you a complete kit that performs all the functions of a standard taper kit and more, but on a true microscale! The Wheaton Micro Kit®, complete with grease-free Wheaton Connections®, simplifies many organic techniques and is helpful in synthesis and the study of reaction mechanisms. Microscale techniques, which are consistent with current analytical techniques, have the following important attributes:

Safe. Working on a microscale means less exposure to toxic and radioactive materials and possible carcinogens.

Environmentally friendly. Disposal problems are greatly reduced because fewer chemicals are needed.

Economical. The consumption of expensive chemicals can be cut drastically. Breakage from frozen joints is eliminated. Disposable vials can be used for reaction vessels.

Components are assembled with grease-free, screw-threaded Wheaton Connections®. Wheaton Connections® that are made from phenolic have a working temperature of up to 180°C. All caps have open tops and Teflon® faced septa for maximum versatility. Flask sizes range from 1 mL to 10 mL. Graduated V Vials® with interior conical bottoms are included for reaction and collection vessels. The 1 mL V Vial is graduated at the 0.1 mL level.

The compact design of Wheaton grease-free, microscale apparatus makes it possible to join components on a desktop. Viton® “O” rings are included in the kit for gas inlet tubes or thermometers. Because components hold themselves together, only minimal clamping is necessary.



Component and Replacement Parts110008 Claisen Adapter, 13-425 Screw Threads 1 1125478 Wheaton Connection®, 13-425 to 13-425 4 6125480 Wheaton Connection®, 13-425 to 20-400 1 6962515 Hose Connector, 13-425 2 6161978 Chromatography Connection,13-425 to 1/4 x 28 1 1168378 Vacuum Adapter, 13-425 and 20-400 Screw Threads 1 1224952 4 mL Vial with 13-425 Cap 1 40333927 Water Jacketed Condenser, 13-425 Screw Threads 1 1265318 Air Condenser, Drying Tube, Chromatography Column, 50 mm, 13-425 Screw Threads 2 1265320 Air Condenser, Drying Tube, Chromatography Column, 100 mm, 13-425 Screw Threads 1 1420836 Teflon® Filter with Support 5 30434320 10 mL Flask with 20-400 Cap 2 6903007 Spin bars 2 6903061 Spin Vanes for 1 mL V Vial 2 6903063 Spin Vanes for 2, 3 and 5 mL V Vials 2 6W986294NG 1 mL Graduated V Vial with 13-425 Cap 1 12W986297NG 3 mL Graduated V Vial with 20-400 Cap 2 12W986299NG 5 mL Graduated V Vial with 20-400 Cap 2 12620189 Hickman Still, 13-425 and 20-400 Screw Threads 1 1WF033112 Connecting Adapter, 13-425 Screw Threads 2 1

•

•

••

72


Components in the Semi-Micro Kit are sized comparably to standard taper 19/22 glassware but have been designed for use with grease-free Wheaton Connections®. They are ideal for schools and universities because there is no need to change existing textbook methods. These kits are also ideal for industrial applications such as medicinal chemistry.

Safe. Working on a microscale means less exposure to toxic and radioactive materials and possible carcinogens.

Environmentally friendly. Disposal problems are greatly reduced because fewer chemicals are needed.

Economical. The consumption of expensive chemicals can be cut drastically. Breakage from frozen joints is eliminated. Disposable vials can be used for reaction vessels.

Components are assembled with grease-free, screw-threaded Wheaton Connections®. Wheaton Connections® that are made from phenolic have a working temperature of up to 180°C. All caps have open tops and Teflon® faced septa for maximum versatility. Flask sizes range from 1 mL to 10 mL. Graduated V Vials® with interior conical bottoms are included for reaction and collection vessels. The 1 mL V Vial is graduated at the 0.1 mL level.

The compact design of Wheaton grease-free, microscale apparatus makes it possible to join components on a desktop. Viton® “O” rings are included in the kit for gas inlet tubes or ther-mometers. Because components hold themselves together, only minimal clamping is necessary.



Component and Replacement Parts 110012 Claisen Adapter, 20-400 Screw Threads 1 1123892 Connecting Adapter, 20-400 Screw Threads 1 1125480 Wheaton Connection®, 13-425 to 20-400 1 6125486 Wheaton Connection®, 20-400 to 20-400 6 6165996 Thermometer Adapter, 20-400 1 1168388 Vacuum Adapter, 13-425 and 20-400 Screw Threads 1 1333952 West Condenser, 200 mm, 13-425 and 20-400 Screw Threads 1 1385792 Distilling Column, 200 mm, 13-425 and 20-400 Screw Threads 1 1439195 Round-Bottom Flask, 25 mL, 20-400 1 1439215 Round-Bottom Flask, 50 mL, 20-400 1 1439234 Round-Bottom Flask, 100 mL, 20-400 1 1439264 Round-Bottom Flask, 250 mL, 20-400 1 1677670 Separatory Funnel with Teflon® Lined Cap, 125 mL, 20-400 1 1962515 Hose Connector, 13-425 2 6964453 Bleed Tube 1 12

•

•

••

For applications using Wheaton Chemistry Kits see pages 163-164.

73


Dessicators

Rugged, heavy soda-lime glassLift off cover for storage above flangeSilicone sealing ring for greaseless sealingMaximum vacuum of 27” Hg (914 hPa)Temperature range: 15°C to 80°C

Wheaton vacuum desiccators come complete with desiccator jar, cover, sleeve valve, silicone sealing ring, and aluminum plate. Place your sample inside the desiccator and apply vacuum to the side arm sleeve valve. The cover of the dessicator jar has a molded arrow on the top for alignment to the open position. Simply turn the sleeve valve to close the desiccator. To open the cover, simply turn sleeve valve to the open position. Once equilibrated, the cover will easily lift off. The desiccator is manufactured from soda-lime glass.

To prevent freezing and increase performance, lubricating the standard taper sleeve valve with silicone-based vacuum grease is recommended. A 1/4” to 3/8” vacuum hose (not included) is used to connect the desiccator to vacuum or gas sources.

Cat. No. Opening Size (mm) Joint Size (mm) Overall H (mm) Plate Dia (mm) Qty/Case

365882 100 40/35 170 89 1365884 150 40/35 225 140 1365886 250 55/38 325 223 1

•••••

Why use a vacuum desiccator?

Oven drying is the most common method of removing moisture from solids. However, this technique is not suitable for substances that are heat labile or tend to decompose. Vacuum desiccators are versatile and are equipped with a sidearm for connection to a vacuum source.

The contents of the sealed evacuated desiccator are maintained in a dry, reduced-pressure atmosphere. Dried materials are stored in desiccators while cooling in order to minimize the uptake of moisture. After releasing vacuum, the desiccator cover can be removed vertically without disturbing the sample. Desiccators can be used for cooling oven-dried samples while keeping the samples dry.

Hygroscopic materials should be stored in containers having tight fitting closures. Deliquescent samples and effervescent pharmaceuticals are prime examples.

Handling dried objects with one’s fingers can result in transfer of weighable quantities of water or oil from the skin. While weighing desiccator dried materials, forceps or tongs should be used.

•

•

74Dessicators


A chemically resistant Viton® rubber sealing ring is recommended when using common or corrosive organics in the Wheaton Dry Seal® vacuum desiccators.

Cat. No. Desiccator Opening Size (mm) Qty/Case

366012 100 1366014 150 1366016 250 1

Replacement aluminum plate for Wheaton Dry-Seal® vacuum desiccators

Cat. No. Desiccator Opening Size (mm) Outside Diameter (mm) Qty/Case

366042 100 89 1366044 150 140 1366046 250 223 1

These packs are designed for use in all desiccators including the Wheaton Dry-Seal® 150 and 250 mm opening vacuum desiccators. The desiccant pack is placed in the bottom of the desiccator under the aluminum plate to preserve an anhydrous atmosphere within the desiccator. The desiccant is totally enclosed in fabric and perforated plastic for a dust-free environment. It comes supplied in a sealed foil package.

To reactivate the desiccant pack: When pack turns from blue to pink, simply place the entire packet with perforated side down into vented oven and heat for approximately three hours at 150°C or until the desiccant returns to its original blue color.


366074 150 1366076 250 1

•

Replacement jar (base) for Wheaton Dry-Seal® desiccators

Cat. No. Opening Size (mm) Qty/Case

365822 100 1365824 150 1365826 250 1

Replacement cover for Wheaton Dry-Seal® vacuum desiccators

Cat. No. Opening Size (mm) Joint Size (mm) Qty/Case

365892 100 40/35 1365894 150 40/35 1365896 250 55/38 1

Replacement sleeve valve for Wheaton Dry-Seal® vacuum desiccators

Cat. No. Joint Size (mm) Qty/Case

365904 40/35 1365906 55/38 1

Replacement retaining rings for Wheaton Dry-Seal® vacuum desiccators

Cat. No. For Sleeve Valve Size Qty/Case

365914 40/35 6365916 55/38 6

Replacement silicone ring for Wheaton Dry-Seal® vacuum desiccators

These are the replacement sealing rings for the original Wheaton Dry-Seal® vacuum desiccator.


366022 100 1366024 150 1366026 250 1

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75


Filtration

Connection to Flask/Bottle

Silicone or neoprene stoppers are the most common connectors used to connect the filtration assembly to an Erlenmeyer style flask, and are appropriate for most routine filtration studies of aqueous solutions. However, we do not recommend stoppers when filtering organic or most corrosive solutions or when preparing HPLC mobile phase.

We recommend filtration assemblies with standard taper 40/35 outer joint connections when filtering corrosive solutions and organic solvents. This connection style is also ideal when preparing HPLC mobile phase, since the “all-glass” connection eliminates phthalate contamination that can occur when using silicone or neoprene stoppers.

W heaton Science Products offers a wide variety of membrane filter support glassware for most vacuum filtration assemblies.

To select the right support glassware for your need:

Choose 25, 47 or 90 mm, according to the diameter of your filter membrane.Select from fritted glass, stainless steel, or a PTFE supportChoose the appropriate connection for the flask or bottle you will use to collect the filtrate.

Following is the basic information you will need to select the most appropriate glassware for your application.

Filter Size

25 mm Support Glassware is recommended when filtering small volumes of liquids for particulate or biological contamination studies.

We recommend 47 mm Support Glassware when analyzing up to 500 mL of liquids for particulate or biological contamination, water/wastewater analysis (Reference: Standard Methods for the Examination of Water and Wastewater, 21st Edition, 2005), sterility testing of pharmaceuticals using the Membrane Filtration Method (Reference: U.S. Pharmacopeia), and for particulate-free or sterile filtrate preparation. We recommend 90 mm Support Glassware when filtering large sample volumes, liquids with a heavy particulate load and viscous liquids when a high flow rate is required. The larger filter area can provide filtration rates four times faster than 47 mm assemblies.

Support Style

We recommend fritted glass supports for general membrane filtration analysis of samples for microbiological or particulate contamination. This support style is appropriate when using gridded filters because the glass frit provides the most even distribution of the sample through the filter membrane. All of the fritted glass support bases have a coarse (40-60 μm) porosity. We recommend stainless steel supports when filtering viscous or proteinaceous solutions to give the maximum flow rate. This support style is also ideal for producing ultra-clean filtrate, since the stainless steel screen will not shed particles into the filtrate.

For Care and Cleaning, see page 81.

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Cat. No. Filter Size Filter Area Prefilter Size Support Material Support Porosity Connection to Flask/Bottle

419325 25 mm ~ 2.5 cm2 20 mm Fritted Glass 40-60 μm No. 5 Stopper 419327 25 mm ~ 2.5 cm2 20 mm Stainless Steel 100 mesh No. 5 Stopper 419347 47 mm ~ 9.6 cm2 35 mm Fritted Glass 40-60 μm No. 8 Stopper 419360 47 mm ~ 9.6 cm2 35 mm Stainless Steel 100 mesh No. 8 Stopper 419370 47 mm ~ 9.6 cm2 35 mm Fritted Glass 40-60 μm No. 8 Stopper 419380 47 mm ~ 9.6 cm2 35 mm Fritted Glass 40-60 μm 40/35 Joint419385-47 47 mm ~ 9.6 cm2 35 mm Fritted Glass 40-60 μm 40/35 Joint 419400-47 47 mm ~ 9.6 cm2 N/A Fritted Glass 40-60 μm 40/35 Joint 419520-47 47 mm ~ 9.6 cm2 35 mm Fritted Glass 40-60 μm GL 45 Thread 419530-47 47 mm ~ 9.6 cm2 35 mm Stainless Steel 100 mesh GL 45 Thread 419390 47 mm ~ 9.6 cm2 35 mm Stainless Steel 100 mesh 40/35 Joint 419450 90 mm ~ 38.5 cm2 70 mm Stainless Steel 100 mesh No. 8 Stopper 419451 90 mm ~ 38.5 cm2 70 mm PTFE 0.062" No. 8 Stopper 419452 90 mm ~ 38.5 cm2 70 mm Fritted Glass 40-60 μm No. 8 Stopper 419460 90 mm ~ 38.5 cm2 70 mm Stainless Steel 100 mesh 40/35 Joint 419461 90 mm ~ 38.5 cm2 70 mm PTFE 0.062" 40/35 Joint 419462 90 mm ~ 38.5 cm2 70 mm Fritted Glass 40-60 μm 40/35 Joint

Specifications

76Filtration


Wheaton 25 mm vacuum filtration assemblies are designed to handle small volumes of liquids for analysis of particulate or microbiological contamination. The glass components are manufactured from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements. Flasks have a #2 hose connection for 1/4” (6 mm) I.D. tubing.

This unit is recommended for general filtration. It is supplied with a coarse porosity (40-60 μm) fritted glass support base, 15 mL graduated funnel, anodized aluminum clamp, and a No. 5 silicone stopper.



Components419330 Glass Funnel, 25 mm, 15 mL 1419334 Aluminum Clamp, 25 mm 1419332 Fritted Glass Support Base, 25 mm 1419336 Silicone Stopper, No. 5 6

Accessories419331 Glass Funnel, 25 mm, 50 mL 1635229 Filter Flask, 125 mL, Graduated, No. 5 Stopper Joint 1

This unit is designed for filtering viscous or proteinaceous solutions or to produce ultraclean filtrate. The assembly is supplied complete with a 316 stainless steel support with 100 mesh screen, PTFE support screen gasket, glass support base, 15 mL graduated funnel, anodized aluminum clamp and a No. 5 silicone stopper.



Components419330 Glass Funnel, 25 mm, 15 mL 1419334 Aluminum Clamp, 25 mm 1419337 Stainless Steel Support Screen, 25 mm 1419338 Support Screen Gasket, 25 mm, Teflon® 6419333 Glass Base, 25 mm, for S.S. Support 1419336 Silicone Stopper, No. 5 6

Accessories419331 Glass Funnel, 25 mm, 50 mL 1635229 Filter Flask, 125 mL, Graduated, No. 5 Stopper Joint 1

419325

419327

When using a vacuum filtration assembly, a second flask should be connected between the filtering flask and the vacuum source. This helps prevent the accidental entry of the filtrate into the vacuum pump.

77


Filtration

The 47 mm filtration assemblies are designed to handle up to 500 mL of liquid for analysis of particulate or microbiological contamination. The glass components are manufactured from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements. Flasks have a #2 hose connection for 1/4” (6 mm) I.D. tubing.

This unit is recommended for general filtration. It is supplied with a coarse porosity (40-60 μm) fritted glass support base, 300 mL graduated funnel, anodized aluminum clamp, and a No. 8 silicone stopper.



Components419350 Glass Funnel, 47 mm, 300 mL 1419354 Aluminum Clamp, 47 mm 1419352 Fritted Support Base, 47 mm 1419356 Silicone Stopper, No. 8 6

Accessories419410 Glass Funnel, 47 mm, 100 mL 1419415 Glass Funnel, 47 mm, 500 mL 1419420 Glass Funnel, 47 mm, 1000 mL 1635232 Filtration Flask, 1 L, Graduated, No. 8 Stopper Joint 1635233 Filter Flask, 2 L, Graduated, No. 8 Stopper Joint 1635245 Filter Flask, 1 L, Safety Coated, No. 8 Stopper Joint 1

We recommend this unit for autoclaving with the filter in place. The Teflon® coating prevents the filter from adhering to the ground glass surfaces. This unit is supplied complete with a Teflon® faced, coarse porosity (40-60 μm) fritted glass support base, a Teflon® faced 300 mL graduated funnel, an anodized aluminum clamp, and a No. 8 silicone stopper.



Components419374 Glass Funnel, Teflon® Faced, 47 mm, 300 mL 1419354 Aluminum Clamp, 47 mm 1419372 Fritted Glass Support Base, Teflon® faced, 47 mm 1419356 Silicone Stopper, No. 8 6

Accessories635232 Filtration Flask, 1L, Graduated, No. 8 Stopper Joint 1635233 Filter Flask, 2 L, Graduated, No. 8 Stopper Joint 1635245 Filter Flask, 1L, Safety Coated, No. 8 Stopper Joint 1

This unit is designed for filtering viscous or proteinaceous solutions or to produce ultraclean filtrate. The assembly is supplied complete with a 316 stainless steel support with 100 mesh screen, PTFE support screen gasket, support base, 300 mL graduated funnel, anodized aluminum clamp and a No. 8 silicone stopper.



Components419350 Glass Funnel, 47 mm, 300 mL 1419354 Aluminum Clamp, 47 mm 1419364 Stainless Steel Support Screen, 47 mm 1419366 Support Screen Gasket, 47 mm 6419362 Glass Base, 47 mm, for Stainless Steel Support 1419356 Silicone Stopper, No. 8 6

Accessories419410 Glass Funnel, 47 mm, 100 mL 1419415 Glass Funnel, 47 mm, 500 mL 1419420 Glass Funnel, 47 mm, 1000 mL 1635232 Filtration Flask, 1 L, Graduated, No. 8 Stopper Joint 1635233 Filter Flask, 2 L, Graduated, No. 8 Stopper Joint 1635245 Filter Flask, 1 L, Safety Coated, No. 8 Stopper Joint 1

419347

419370

419360

78Filtration


Wheaton “all-glass” filtration assemblies are recommended for the routine filtration of organic solvents, for corrosive liquids and for the removal of particulates from HPLC solvents. The ground glass connection eliminates the phthalate contamination that can occur when you use either silicone or neoprene stoppers. Because the drip tip on the support bases extends below the hose connection, the filtrate cannot enter the vacuum line. All units have a #2 hose connection for 1/4” (6 mm) I.D. flexible tubing. We manufacture the glass components from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements.

We recommend this unit for general filtration. It comes complete with a coarse porosity (40-60 μm) fritted glass support base, graduated funnel, anodized aluminum clamp and 1 L filter flask.


419380 Complete Assembly with 300 mL Funnel, 1 L Flask 1419385-47 Complete Assembly with 500 mL Funnel, 2 L Flask 1

Components419350 Glass Funnel, 47 mm, 300 mL 1419415 Glass Funnel, 47 mm, 500 mL 1419354 Aluminum Clamp, 47 mm 1419382 Fritted Glass Support Base, 47 mm, with 40/35 Outer Joint 1635525 Filter Flask, 1 L, with 40/35 Inner Joint 1635526 Filter Flask, 2 L, with 40/35 Inner Joint 1

Accessories419410 Glass Funnel, 47 mm, 100 mL 1419420 Glass Funnel, 47 mm, 1000 mL 1635527 Filter Flask, 1 L, with 40/35 Inner Joint, Safety Coated 1

This unit can filter viscous or proteinaceous solutions or produce an ultra clean filtrate. The assembly comes complete with a 316 stainless steel support with 100 mesh screen, Teflon® support screen gasket, glass support base, 300 mL graduated funnel, anodized aluminum clamp and 1 L filter flask.



Components419350 Glass Funnel, 47 mm, 300 mL 1419354 Aluminum Clamp, 47 mm 1419364 Stainless Steel Support Screen, 47 mm 1419366 Support Screen Gasket, 47 mm 6419392 Glass Base, 47 mm, with 40/35 Outer Joint, for Stainless Steel Support 1635525 Filter Flask, 1 L, with 40/35 Inner Joint 1

Accessories419410 Glass Funnel, 47 mm, 100 mL 1419350 Glass Funnel, 47 mm, 300 mL 1419415 Glass Funnel, 47 mm, 500 mL 1419420 Glass Funnel, 47 mm, 1000 mL 1635527 Filter Flask, 1 L, with 40/35 Inner Joint, Safety Coated 1

This unit eliminates the traditional “pour and wait” filtration method. When you apply a vacuum, the transfer adapter draws the solvent directly from the reagent bottle. When the filtration is complete, you can close the valve on the transfer adapter to degas the solvent. The assembly comes complete with a coarse porosity (40-60 μm) fritted glass support base, Teflon® transfer adapter, anodized aluminum clamp and 1 L filter flask.


419400-47 Complete Unit 1

Components419620 Filtration Transfer Adapter, Complete 1419354 Aluminum Clamp, 47 mm 1419382 Fritted Glass Support Base, 47 mm, with 40/35 Outer Joint 1635525 Filter Flask, 1 L, with 40/35 Inner Joint 1

419385-47

419390

419400-47

79


Filtration

419530-47

These filtration assemblies are designed to collect the filtrate directly into a standard laboratory bottle. The connection to the bottle is made with a polybutylene terephthalate (PBT) cap and a Teflon®-faced silicone sealing ring. The glass components are manufactured from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements.

We recommend this unit for general filtration. It comes complete with a coarse porosity (40-60 μm) fritted glass support base, 300 mL graduated funnel, anodized aluminum clamp, and a 1 L reservoir.



Components419350 Glass Funnel, 47 mm, 300 mL 1419354 Aluminum Clamp, 47 mm 1419502 Filtration Base, 47 mm with Vacuum Fitting 1419501 Quick Disconnect Adapter 12240755 PBT Cap, 45 mm, with 39 mm Opening 10240780 Teflon®/Silicone Sealing Ring, for 45 mm cap, 26 mm I.D. 10W264730 Reservoir, 1 L 1

This unit is designed to filter viscous or proteinaceous solutions or produce ultra clean filtrate. The assembly comes complete with a 316 stainless steel support with 100 mesh screen, Teflon® support screen gasket, glass support base, 300 mL graduated funnel, anodized aluminum clamp and 1 L reservoir.



Components419350 Glass Funnel, 47 mm, 300 mL 1419354 Aluminum Clamp, 47 mm 1419364 Stainless Steel Support Screen, 47 mm 1419366 Support Screen Gasket, 47 mm 6419501 Quick Disconnect Adapter 12240755 PBT Cap, 45 mm, with 39 mm Opening 10240780 Teflon®/Silicone Sealing Ring, for 45 mm Cap, 26 mm I.D. 10W264730 Reservoir, 1 L 1WF034590 Filtration Base for Stainless Steel Support, 47 mm with Vacuum Fitting 1

The filtration adapter assembly makes it easier to connect standard 47 and 90 mm filtration assemblies with stopper connections to a standard laboratory bottle with a GL45 thread. The adapter replaces the stopper to make the connection to the bottle. A GL32 PBT cap with a Teflon® faced silicone sealing ring makes the connection to the filtration assembly. A GL45 PBT cap with Teflon® faced silicone sealing ring then makes the connection to the bottle. This unit comes complete with a PBT hose connection for use with 1/4” (6 mm) I.D. flexible tubing.



Component Parts240754 PBT Cap, GL32, with 19 mm Opening 10240776 Teflon®/Silicone Sealing Ring, for 32 mm Cap, with 16 mm Opening 5419506 Glass Adapter Only 1419501 Quick Disconnect Adapter 12240755 PBT Cap, GL45, with 39 mm Opening 10240780 Teflon®/Silicone Sealing Ring, for 45 mm Cap, 26 mm I.D. 10

419520-47

419505

80Filtration


We recommend 90 mm filtration assemblies for large sample volumes, liquids with a heavy particulate load and viscous liquids when you require a high flow rate. Because of its larger filter area, the 90 mm assembly can provide filtration rates up to four times faster than 47 mm assemblies. Wheaton Science Products 90 mm filtration assemblies use a unique screw collar design for connecting the funnel to the support base. Compared to the traditional aluminum clamp, this design provides a more even seal of membrane filter in place. The glass components are manufactured from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements. Flasks have a #2 hose connection for 1/4” (6 mm) I.D. tubing.

We recommend these filtration assemblies for routine filtration of aqueous solutions. The No. 8 silicone stopper makes the connection to a standard 1 liter Erlenmeyer-style filtration flask. Each assembly comes complete with a 1 L funnel with screw collar, glass support base, filter support (either fritted glass, stainless steel or Teflon®) and a No. 8 silicone stopper.


419450 Complete Unit with Stainless Steel Support 1419451 Complete Unit with Teflon® Support 1419452 Complete Unit with Removable Glass Frit 1

Components419470 Glass Funnel, 90 mm, 1 L, with Screw Collar 1WF030089 Glass Frit, 90 mm 1419471 Stainless Steel Support, 90 mm 1265422 Teflon® Frit, 90 mm 1419455 Support Base, 90 mm 1419356 Silicone Stopper, No. 8 6

Accessories635232 Filtration Flask, 1 L, Graduated, No. 8 Stopper Joint 1635245 Filter Flask, 1 L, Safety Coated, No. 8 Stopper Joint 1

We recommend these “all-glass” filtration assemblies for the routine filtration of organic solvents, for corrosive liquids, and for the removal of particulate from HPLC solvents. The ground glass connection eliminates the phthalate contamination that can occur when you use either silicone or neoprene stoppers. Because the drip tip on the support base extends below the hose connection, the filtrate cannot enter the vacuum line. These assemblies require a filtration flask with a standard taper 40/35 inner joint (listed below).


419460 90 mm Filter Assembly with Stainless Steel Support 1419461 90 mm Filter Assembly with Teflon® Support 1419462 90 mm Filter Assembly with Removable Glass Frit 1

Components419470 Glass Funnel, 90 mm, 1 L, with Screw Collar 1WF030089 Glass Frit, 90 mm 1419471 Stainless Steel Support, 90 mm 1265422 Teflon® Frit, 90 mm 1419465 Support Base, 90 mm 1

Accessories635525 Filter Flask, 1 L, with 40/35 Inner Joint 1635526 Filter Flask, 2 L, with 40/35 Inner Joint 1635527 Filter Flask, 1 L, Safety Coated, with 40/35 Inner Joint 1

Wheaton makes Erlenmeyer-style flasks from heavy-wall glass to give them the mechanical strength needed for vacuum filtration. The side arms are #2 hose connections that accept standard 1/4” (6 mm) I.D. flexible tubing for connection to a vacuum source. These flasks are manufactured from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements. You should connect a second flask between the filtering flask and the vacuum source to prevent entry of the filtrate into the vacuum source.


635229 Filter Flask, 125 mL, Graduated, No. 5 Stopper Joint 1635232 Filtration Flask, 1 L, Graduated, No. 8 Stopper Joint 1635233 Filter Flask, 2 L, Graduated, No. 8 Stopper Joint 1

This filter flask is coated on the outside with Wheaton’s plastisol safety coating, which is autoclavable (121°C, 15 psi, 15 minutes). The flask has a #2 hose con-nection that accepts 1/4” (6 mm) I.D. flexible tubing for connection to a vacuum source.


635245 Filter Flask, 1 L, Safety Coated, No. 8 Stopper Joint 1

The all-glass transfer funnel is ideal for transferring small volumes (up to 300 mL) of sample to a filtration assembly. The funnel is manufactured from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements.


419430 Glass Transfer Funnel, 300 mL 1

Safety

Safety

CoatedCoated

81


Filtration

Carefully inspect glassware before using it under vacuum. Repair or replace any glassware that is scratched, chipped, cracked or broken. Also, always use adequate safety shielding when working with glass under vacuum.

Fritted filtration glassware is designed for vacuum filtration. If you use it for pressure filtration, do not let the maximum pressure differential on the fritted disk exceed 15 lb/in2 (1 kg/cm2).

Do not subject filtration glassware with a fritted disk to sudden changes in temperature or to a direct flame. Excessive temperature differences between the fritted disk and its surrounding glass causes internal strain to develop in the glassware, which can lead to fracturing of the filtration base. Place fritted glassware in a cold drying oven or autoclave. Do net let the speed of heating or cooling exceed 8°C per minute. Before filtering hot liquids, slowly bring the filtration base up to the temperature of the liquid in a drying oven or hot cabinet. Slowly heat wet fritted support bases to 80°C and dry for one (1) hour before increasing the temperature.

When using a vacuum filtration assembly, connect a second flask between the filtering flask and the vacuum source. This helps prevent the accidental entry of the filtrate into the vacuum pump.

Fritted Support Bases. Wash a new fritted glass filtration base with hot hydrochloric acid, following with several rinses of either distilled or high-quality deionized water. This procedure removes dust particles and powdered glass from the pores of the fritted disk. Pull the acid wash and water rinses through the fritted disk with a good vacuum. Start each successive water rinse only after the preceding one has been completely pulled through.

If no precipitate has entered the pores of the fritted disk, it may be sufficient to rinse the surface of the fritted disk under the tap or with a rinse bottle. Then wipe the fritted disk surface clean with a small brush or squeegee. Do not use paper towels to dry the fritted disk, since this can force particulates into the pores of the disk.

Many precipitants can be removed from the pores of the fritted disk by back-flushing. Connect the stem of the filtration support base to a water tap with a piece of rubber tubing and allow the water to run backwards through the fritted disk. Warning: The water pressure must not exceed 15 lb/in2 (1 kg/cm2).

When working with biological compounds, use sulfuric acid with a nitrate or perchloric addition as a cleaning agent. Avoid dichromate sulfuric acid as a cleaning agent to prevent the reduction formation of trivalent chromium compounds. Hot, concentrated phosphoric acid and hot alkaline solutions attack the surface of the glass and therefore are not suitable for cleaning.

Stainless Steel Screens. Stainless steel screens can be washed with a laboratory detergent with an initial rinse with tap water and a final rinse with distilled water. Hydrochloric acid is not recommended for cleaning as this can promote corrosion of the stainless steel.

Aluminum Clamps. The aluminum clamps have been hard-anodized to prevent corrosion. A wash with a good laboratory detergent followed by an initial rinse with tap water and a final rinse with distilled water should be adequate.

For additional safety in the laboratory, use Wheaton Safety Coated Bottles. They are ideal for mobile phase reservoirs, for storing reagents, chemicals and for collecting waste materials. For more information see pages 15-17.

82Solids Determination


Imhoff Cone with Rack

Used to determine settleable solidsManufactured from styrene-acrylonitrileRemoval plug allows for easy cleaningImhoff cone rack can be purchased separately

This unit is designed for volumetric determination of settleable solids in surface and saline waters as well as domestic and industrial wastes. The cone is externally graduated from the plug up to 1000 mL: 0 to 1 mL x 0.1 mL; 1 to 10 mL x 0.5 mL; 10 to 40 mL x 1 mL, 40 to 100 mL x 2 mL; with lines and numbers at 250, 500 and 1000 mL. The Imhoff Cone is manufactured from styrene- acrylonitrile and comes with a low-density polyethylene plug. To clean, simply remove the leak-resistant screw cap and plug. Reference: Standard Methods for the Examination of Water and Wastewater, 21st Edition (2005), Method 2540 F. The 3 and 4-place Imhoff Cone Racks are constructed of plated, heavy-gauge steel to provide long life.

Cat. No. Description Size (mL) Top Dia x Length (mm) Qty/Case

W990800 Imhoff Cone 1000 108 x 451 4

Imhoff Cone Racks990760 3-Place Rack — — 1990760-4 4-Place Rack — — 1

Aluminum weighing dishesIdeal for milligram weighingUse with liquids or solids

These lightweight, low tare (0.15 g for 11 mm and 0.45 g for 20 mm) dishes are ideal for milligram weighing. The smooth aluminum surface ensures complete transfer. Use of balance-type forceps enables easy grasping. Liquids or solids may be transferred to this micro dish to be weighed.

Cat. No. Dia x H (mm) Qty/Case

370790 11 x 6 1000370792 20 x 8 1000

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Wheaton has many products for environmental testing and for sample and liquid handling. Refer to the sections below when searching for these specific items.

BOD Bottles page 3

Bottles for Sample Collection pages 7 - 28

Bottle Top Dispensers pages 99-100

Chemistry Glassware pages 67-80

Coliwasa & Other Sample Collection Devices page 129

EPA Vials & Other Sample Collection Vials pages 142-152

Imhoff Cone for Solids Determination page 82

Pipettors with volumes up to 10 mL pages 111-116

Safety Coated Bottles pages 16 - 17

TCLP Extraction Bottles page 5

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83


Autosampler VialsWheaton Science Products chromatography vials and accessories are your assurance of reproducible quality. Not all autosampler vials are alike…so be sure to specify Wheaton for the best in quality, value and reliability.

Wheaton vials are available in crimp, snap/crimp and screw cap finish. The information below explains the difference between the different thread finishes on the vials.

Wheaton ABC Vials™ offer several benefits over comparable vials:

Superior thread design provides a more secure seal to the closure40% larger neck opening versus standard opening screw top vials improves sample

accessibility, accommodates wider range of inserts, and reduces autosampler needle damage

ABC Caps are supplied with Wheaton “Super Septa” low extractable vial septa – optimal for GC/MS

Packaged in recyclable PET clamshells to reduce particulate contaminationCompatible with most GC autosamplers which accept crimped top vial

Wheaton E-Z Vials® are the autosampler vial of choice in the laboratory market.

40% larger neck opening versus standard opening vials improves sample accessibility, accommodates wider range of inserts, and reduces autosampler needle damage

Larger capacity: 1.8 mL versus 1.5 mLE-Z convenience packaging – vials are shrink-wrapped in trays of 100Crimp, snap-crimp, or thread finish

Convenient seals remove in a snap! Available in a wide variety of liners and colors.

Positive seal and easy removalCrimping and decapping tools not requiredLarger target area for easier needle penetration

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Vial Size (mm) 8 x 40 12 x 32 12 x 32 12 x 32 12 x 32 12 x 32 12 x 32 15 x 45 15 x 45Vial Type Shell Vial Shell Vial Crimp Finish Snap/Crimp ABC 9mm Screw Thread 8-425 Screw Thread 10-425 Screw Thread 13-425 Screw Thread Shell Vial

Mfr. & ModelAgilent GC CTC Combi PAL/GC PAL - - • • • - - - -7890A - - • • • - - - -7673/7683 - - • • • - - - -7670A/7671A - - • • • - - - -6820/6850/6890N - - • • • - • • -5975C - - • • • - - - -5890/7985A/6890 - - • • • - - - -Agilent LC 1200 Series - - • • • - - - -1100 Series Prep - - • • • - - - -1100 Series Standard - - • • • - - - -1050 - - • • • • - - -1090 - - • • • - - - -Alcott Chrom 719D - - • • • • • - -718AL - - • • • • • - -728 - - - - - - - - -Alltech Associates 580 - - • • • • • -570 - - • • • • • • -Amersham Pharmacia Biotech (division of Applied Biosystems Group) 2157-010 - - • • - • - - -2457-020 - - - - - - - - -Beckman Coulter 501/502/507 - - - - - • • - -504 - - - - - - - • -508 - - - - - - - • -Bruker / Daltronics LC 51 - - - - - - - • •CE Instruments (division of Thermo Fisher Scientific) 42 Place Tray - - • • - - - • •60 Place Tray - - - - - - - - -AS105 Tray - - • • - - - - -AS 200 Tray - - - - - - - - -

84Autosampler Vials


Vial Size (mm) 8 x 40 12 x 32 12 x 32 12 x 32 12 x 32 12 x 32 12 x 32 15 x 45 15 x 45 Vial Type Shell Vial Shell Vial Crimp Finish Snap/Crimp ABC 9mm Screw Thread 8-425 Screw Thread 10-425 Screw Thread 13-425 Screw Thread Shell Vial

Mfr. & ModelDani ALS 86.80 - - • • • • - - -ALS 39.80 - - • • • • - - -Dionex/Gynkotek Gina - - • • - • - - -ALI-100 - - - • • • - - -AS 509 - - - • • • - - -Dynatech/Precision WPS-3000 - - • • • • - - -42 Place Tray - - • • • • - - -60 Place Tray - - - - - - - - -LC2000 - - - - • - - - -231 XL, 232 XL - - • • • • - - -ESA/LC Packing Model 542 - - • • • • - - -Midas - - • • • • - - -Gilson 233 Sample Changer - - - - - - - - -231 XL/232XL/ 233XL - - • • • • - - -GX-271/281/ - - • • • • - - -Aspec XL - - • • • • - - -Hitachi L2200 - - • • • • - - -L7250 - - • • • • • • -L8800 - - - • • - - - -L7200 • - - • • - • •AS-2000 - - • • • • - - -AS-4000 - - • • • • - - -Jasco AS-2050/2055/2057 - - • • • • - - -AS-2059 - - • • • • - - -AS-1555/1555-10 - - • • • • - - -AS-1559 - - • • • • - - -851-AS, AS-900 - - • • • • - - -LC 800/900 Series - - - - - • - - -Leap Technologies CTC DI PAL - - • • • • - - -CTC Combi PAL - - • • • • - - -CTC A200E - - • • • • - - -CTC HTS PAL - - • • • • - • -CTC HTS Twin PAL - - • • • • - - -CTC HTC PAL - - • • • • - - -CTC LC mini PAL - - • • • • - - -CTC A2000S - - • • • • - - -A200LC - - • • - - - - -Perkin Elmer Claris 500, 600 - - • • • • - - -Autosystem - - • • • • - - -Autosystem XL - - • • • • - - -Series 200 - - • • • • - - -Integral 4000 - - • - - - - - -ISS 100, ISS 200 - - • • • - - - -LC 600 42 Place Tray - - • • • - - - -Phillips/ Pye UNICAM 4710 - - • • - • - - -4700LC-GC/S4/S8 - - - - - - - - -4247 - - • • - • - - -C-XP - - - - - - - • •Shimadzu GC AOC-20i/AOC-20s - - - - - • • • •AOC-8B - - - - - • - - -AOC-5000 • - - - - • - - -AOC-14/AOC-17 - - - - - • - • •Shimadzu HPLC SIL-HTa/SIL-HTc • - - • • • • • -SIL-10ADVP • - - • • • • • -SIL-10A/SIL-10Ai/SIL-10Ap • - - • • • • • -SIL-6B/SIL-9A/SIL-8A - - - - - • - - -SIL-10AD - - - • • • • - -Promis • - • • • • - - -Spark Holland Reliance - - • • • • - - -Midas - - • • • • - - -Marathon • - • • • • - - -Triathon • - • • - • - - -Thermo Scientific (division of Thermo Fisher Scientific) AS1000/AS3000/AS3500 - - • • - • - - -TriPlus AS/HS/DUO - - • • - • - - -Surveyer Plus / Plus Lite - - • • - • - - -Accela - - • • - • - - -8875/8880 - - • • - • - - -Varian GC CP-8400/CP-8410 - - • • • • - - -8200CX - - - - • - - - -Ultra GC/MS - - - - • - - - -8100/8200 - - • • • • • - -Varian LC 9100/9090/9095 - - • • - • - - -ProStar 400/410 - - - • • - - - -ProStar 420/430 - - - • • - - - -Marathon • - • • • • - - -Waters LC Acquity - - - • • • • - -Alliance • - - • • • - • •Breeze • - - • • • - • -717Plus • - - • • • - • •2700 Sample Manager - - - • • - - - -710/712/715 - - • - - - - • •

85


Autosampler Vials

RED - Glass Vials BLUE - Plastic Vials GREEN - Glass/Plastic VialsWheaton chromatography vials and inserts are shown at exact size. The catalog numbers below are color coded by vial material.

8 x 40 mm

225241 (Clear) W225243 (Clear)

225242 (Clear)

225172 (Amber) 225173 (Amber) 225174 (Clear) 225175 (Clear)

225220-01 (Clear) 225221-01 (Amber)

225179 (Clear) 225179-01 (Clear)

225179-02 (Amber) 225179-03 (Amber)

223682 (Clear) 223682-01 (Clear) 223692 (Amber)

223692-01 (Amber)

W225150 (Clear) W225151 (Clear)

W225152 (Amber) W225153 (Amber)

225326 (Clear) 225328 (Amber)

224626 (Clear) 224627 (Clear)

224628 (Amber) 224629 (Amber)

W225900 (Clear) W225910 (Amber)

225120 (Clear) 225121 (Amber) 225124 (Clear)

225180 (Clear) 225185 (Clear) 225190 (Clear) 225200 (Amber)

225195 (Clear) 225205 (Amber)

225235 (Clear) W225237 (Clear) 986211 (Clear)

986212 (Clear) 986281 (Clear) 986282 (Clear) W225187 (Clear) W225186 (Clear) W225181 (Clear) W225149 (Clear) W242805 (Clear)

12 x 32 mm

86Autosampler Vials


224794 (Clear) 224794-01 (Clear) 224795 (Amber)

224795-01 (Amber)

225126 (Clear) 225127 (Amber) 225129 (Clear)

225130 (Clear)

RED - Glass Vials BLUE - Plastic Vials GREEN - Glass/Plastic VialsWheaton chromatography vials and inserts are shown at exact size. The catalog numbers below are color coded by vial material.

15 x 45 mm

225256 (Clear) 225257 (Clear)

W225251 (Clear)

Bottom Spring

225255 (Clear)

Top Spring

225265 (Clear)

Bottom Spring

225258 (Clear) 225259 (Clear)

For Vial with Step

225260 (Clear) 225261 (Clear)

Bottom Spring

225268 (Clear)

Bottom Spring

225350-631 (Clear) 225350-531 (Clear)

Flat Bottom

Limited Volume Inserts

225277 (Clear) 225278 (Clear) 225280 (Clear) 985688 (Clear)

Headspace Vials

87


Autosampler Vials

Shell Vials Limited Volume Insert

Can be used in Waters WISP® 96-position autosamplerChoice of borosilicate glass or polypropylene0.15 or 0.20 mL limited volume insertSnap plug closures available in 5 colorsPurchase vials and caps separately or together in Convenience Packs

These vials are specially designed for use with the Waters WISP® 96-position autosampler. The vials are available in clear borosilicate glass and natural polypropylene. Vials, limited-volume inserts and snap plug caps can be purchased separately or purchase vials and caps together by ordering the Wheaton Convenience Packs. The polyethylene snap plug caps feature a starburst top for easier needle penetration and they are available in natural, red, blue, green and yellow.

Diameter x Height .........................................................................8 x 40 mmVolume: Vial ............................................................................................ 1.0 mL Limited volume vial ..................................................................... 0.7 mL Limited volume insert ..................................................... 0.15 & 0.20 mLCap size ............................................................................................... 8 mm

Cat. No. Description Shelf Pack Qty/Case

Shell VialW225243 1.0 mL Glass Shell Vial, Clear 200 1000225241 1.0 mL Polypropylene Shell Vial 200 1000225242 0.7 mL PP Limited Volume Shell Vial 100 1000

Limited Volume Insert225256 0.20 mL Glass Insert with Bottom Spring — 100W225251 0.15 mL Glass Insert with Bottom Spring — 100

8 mm Snap Plug Caps242800 Natural 200 1000242802 Red 200 1000242804 Blue 200 1000242806 Green 200 1000242808 Yellow 200 1000

Convenience PackW225244 1.0 mL Glass Shell Vial, Plug Cap (Cat. No. 242800) 200 1000

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Choice of borosilicate glass or polypropyleneSnap plug closure available in natural polyethylenePurchase vials and caps separately or together in Convenience Packs

These vials are available in clear or amber borosilicate glass and natural polypropylene. Vials and snap plug caps can be purchased separately or together by ordering the Wheaton Convenience Packs. The polyethylene snap plug caps feature a starburst top for easier needle penetration.

Diameter x Height .......................................................................12 x 32 mmVial Volume ........................................................................................ 2.0 mLCap size ............................................................................................. 12 mm


12 x 32 Shell Vial225120 2.0 mL Glass Shell Vial, Clear 100 1000225121 2.0 mL Glass Shell Vial, Amber 100 1000225124 2.0 mL Polypropylene Shell Vial 100 1000

12 mm Snap Plug Cap242810 12 mm Snap Plug Cap, Natural 100 1000

Convenience PackW225122 2.0 mL Glass Shell Vial, Clear with Plug Cap 100 1000W225123 2.0 mL Glass Shell Vial, Amber with Plug Cap 100 1000W225125 2.0 mL Polypropylene Shell Vial with Plug Cap 100 1000

Vials fit 985800 vial rack. See page 149.

Choice of borosilicate glass or polypropyleneSnap plug closure available in low density polyethylene

These vials are available in clear and amber borosilicate glass and natural polypropylene. Vials and snap plug caps can be purchased separately. The poly-ethylene snap plug caps feature a starburst slit top for easier needle penetration.

Diameter x Height .......................................................................15 x 45 mmVial Volume ........................................................................................... 4 mLLimited Volume Vial ............................................................................... 3 mLCap size ............................................................................................. 15 mm


15 x 45 mm Shell Vial225126 4.0 mL Glass Shell Vial, Clear 100 1000225127 4.0 mL Glass Shell Vial, Amber 100 1000225129 4.0 mL Polypropylene Shell Vial 100 1000225130 3.0 mL Polypropylene Limited Volume Shell Vial 100 1000

15 mm Snap Plug Cap 242815 15 mm LDPE Conical Snap Plug Cap, Natural 100 1000


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Available in

Convenience

Packs

Available in

Convenience

Packs

Available in

Convenience

Packs

Available in

Convenience

Packs

Organize your shell vials in the Wheaton autoclavable polypropylene vial rack (Cat. No. 985750). See page 149 for details.

88Autosampler Vials


E-Z Vials Limited Volume Insert

40% large openingManufactured from borosilicate glassUse with 11 mm aluminum sealsLimited volume insertPurchase vials and caps separately

or together in Convenience Packs

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Diameter x Height ........................................................................12 x 32 mmVial volume ........................................................................................ 1.8 mLLimited volume insert ............................................................ 0.10 - 0.35 mLCrimp Cap size .................................................................................... 11 mm

Wheaton E-Z Vials feature a 40% larger neck opening than standard opening vials. The larger opening improves sample accessibility while reducing auto sampler needle damage. Vial capacity has been increased from 1.5 to 1.8 mL. E-Z Vials are designed for use with 11 mm aluminum seals. E-Z Vials are manufactured from Type I borosilicate glass and are packaged in shrink-wrapped trays of 100 for convenience and cleanliness. For 11 mm Aluminum E-Z Seals see page 89.


E-Z Vial225175 1.8 mL E-Z Vial, Clear 100 1000225174 1.8 mL E-Z Vial, Clear, w/Writing patch 100 1000225172 1.8 mL E-Z Vial, Amber 100 1000225173 1.8 mL E-Z Vial, Amber, w/Writing patch 100 1000

Limited Volume Inserts225255 0.10 mL Glass Insert with Top Spring 100 200225265 0.25 mL Glass Insert with Bottom Spring — 100225257 0.25 mL Polypropylene Insert with Bottom Spring — 100225350-631 0.35 mL Glass Flat Bottom Insert 200 1000

Available in

Convenience

Packs

Available in

Convenience

Packs

Convenience

PacksConvenie

nce

Packs

11 mm E-Z Seals (See page 89)

12 x 32 E-Z Vial® & 11 mm Aluminum E-Z Seal™

E-Z Vials and 11 mm Aluminum Seals are available in convenience packs of 100. The clear and amber vials are made of Type I borosilicate glass. The aluminum seals are available in red, blue, green and natural colors.


12 x 32 mm Clear E-Z Vial with Aluminum Seal


With 0.002 Teflon®/0.038 Red Rubber LinersW225175-01 Natural Aluminum 100W225175-0103 Red Aluminum 100W225175-0104 Blue Aluminum 100W225175-0107 Green Aluminum 100

With 0.002 Teflon®/0.038 Silicone LinersW225175-02 Natural Aluminum 100W225175-0203 Red Aluminum 100W225175-0204 Blue Aluminum 100W225175-0207 Green Aluminum 100

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With 0.002 Teflon®/0.036 Silicone/0.036 Teflon® LinersW225175-03 Natural Aluminum 100W225175-0303 Red Aluminum 100W225175-0304 Blue Aluminum 100W225175-0307 Green Aluminum 100

With 0.002 Teflon®/0.037 Gray Butyl LinersW225175-12 Natural Aluminum 100W225175-1203 Red Aluminum 100W225175-1204 Blue Aluminum 100W225175-1207 Green Aluminum 100

12 x 32 mm Clear E-Z Vial with Patch & Aluminum Seal






12 x 32 mm Amber E-Z Vial with Aluminum Seal






12 x 32 mm Amber E-Z Vial with Patch and Aluminum Seal






89


Autosampler Vials

Manufactured from borosilicate glassUse with 11 mm aluminum E-Z Seals™ Limited volume insertPurchase vials and caps separately or together in Convenience Packs

Diameter x Height ........................................................................12 x 32 mmVial volume ........................................................................................ 1.5 mLLimited Volume Insert ......................................................... 0.10 and 0.25 mLCrimp cap size ................................................................................... 11 mm

These glass vials are the original design used in most autosamplers. Wheaton standard crimp top vials ensure quality and reliability while maintaining sample integrity. They are manufactured from Type I borosilicate glass and are packaged in shrink-wrapped trays of 100 for convenience and cleanliness. For 11 mm Aluminum E-Z Seals see bottom of this page.


12 x 32 Standard Opening Crimp Vial223682 1.5 mL Vial, Clear 100 1000223682-01 1.5 mL Vial, Clear, with Writing Patch 100 1000223692 1.5 mL Vial, Amber 100 1000223692-01 1.5 mL Vial, Amber, with Writing Patch 100 1000

Limited Volume Insert225260 0.10 mL Glass Insert with Bottom Spring — 100225261 0.10 mL Polypropylene Insert with Bottom Spring — 100225350-531 0.25 mL Glass Flat Bottom Insert — 1000

For use with 12 x 32 mm crimp cap vialsLarge target diameterAvailable in 4 colorsChoice of liner


With 0.002 Teflon®/0.038 Red Rubber Liner224211-01 Natural Aluminum 100 1000224211-05 Blue Aluminum 100 1000224211-06 Red Aluminum 100 1000224211-07 Green Aluminum 100 1000

With 0.002 Teflon®/0.038 Silicone Liner224219-01 Natural Aluminum 100 1000224219-05 Blue Aluminum 100 1000224219-06 Red Aluminum 100 1000224219-07 Green Aluminum 100 1000

With 0.002 Red Teflon®/0.036 Silicone/0.002 Red Teflon® Liner224231-01 Natural Aluminum 100 1000224231-05 Blue Aluminum 100 1000224231-06 Red Aluminum 100 1000224231-07 Green Aluminum 100 1000

With 0.002 Teflon®/0.037 Silicone Gray Butyl Liner224235-01 Natural Aluminum 100 1000

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40% larger opening than standard crimp vialsSelect from clear or amber borosilicate glassWith or without writing patchAccepts snap cap (see page 90) or 11 mm aluminum seal (see page 89)Glass and polypropylene low volume inserts availablePurchase vials and snap caps separately or together in Convenience Packs

(page 90)

Diameter x Height ........................................................................12 x 32 mmVial volume ........................................................................................ 1.8 mLLimited volume insert ............................................... 0.10, 0.25 and 0.35 mLSnap Cap/Crimp Cap size ................................................................... 11 mm

These E-Z Vials can use a plastic snap cap closure, which eliminates the need for crimping and decapping tools. The opening is 40% larger than standard 11 mm finish vials. These vials also accept 11 mm aluminum crimp-top E-Z Seals. E-Z Vials are manufactured from Type I borosilicate glass and are packaged in shrink-wrapped trays of 100 for convenience and cleanliness.


E-Z Vial225179 1.8 mL, Clear 100 1000225179-01 1.8 mL, Clear, with Writing Patch 100 1000225179-02 1.8 mL, Amber 100 1000225179-03 1.8 mL, Amber, with Writing Patch 100 1000

Limited Volume Inserts225255 0.10 mL Glass Insert with Top Spring 100 200225265 0.25 mL Glass Insert with Bottom Spring — 100225257 0.25 mL Polypropylene Insert with Bottom Spring — 100225350-631 0.35 mL Glass Flat Bottom Insert 200 1000

See the E-Z Vial with Snap Ring Closures in Convenience Packs listed on page 90.

Eliminate the need for crimping and decapping toolsFits the Wheaton E-Z Vials® with Snap Ring TopManufactured from polyethyleneChoose from 5 colored caps and a variety of septa

Wheaton Snap Caps eliminate the need for crimping and decapping tools. Their unique design is engineered for a positive seal and easy removal. Wheaton Snap Caps are ideal for high-volume, quick turnaround sampling and provide a large target area for needle penetration. They are not recommended for long-term sample retention. Snap Caps are manufactured from polyethylene and are supplied in five different colors with a variety of septa. Caps with starburst slit allow for easy needle penetration.

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Available in

Convenience

Packs

Available in

Convenience

Packs

12 x 32 Crimp Vial w/ E-Z Seals Limited Volume Insert

90Autosampler Vials


12 x 32 E-Z Vials® & 11 mm Snap Caps packaged together

Choice of clear or amber vials with or without writing patch

Select from 5 colored caps and a variety of liners

The E-Z Vials and Snap Caps are available in Convenience Packs of 100. The clear and amber vials are made of Type I borosilicate glass. The Snap Caps are available in red, blue, yellow, green and natural colors.

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12 x 32 mm Clear Vial with Snap CapCat. No. Description Qty/Case

With 0.002 Teflon®/0.038 Red Rubber LinersW224620-01 Natural PE Cap 100W224620-0103 Red PE Cap 100W224620-0104 Blue PE Cap 100W224620-0105 Yellow PE Cap 100W224620-0107 Green PE Cap 100

With 0.002 Teflon®/0.038 Silicone LinersW224620-02 Natural PE Cap 100W224620-0203 Red PE Cap 100W224620-0204 Blue PE Cap 100W224620-0205 Yellow PE Cap 100W224620-0207 Green PE Cap 100

With 0.002 Teflon®/0.036 Silicone/0.002 Teflon® LinersW224620-03 Natural PE Cap 100W224620-0303 Red PE Cap 100W224620-0304 Blue PE Cap 100W224620-0305 Yellow PE Cap 100W224620-0307 Green PE Cap 100

12 x 32 mm Clear Vial with Patch and Snap Cap Cat. No. Description Qty/Case




12 x 32 mm Amber Vial with Snap CapCat. No. Description Qty/Case



With 0.002 Teflon®/0.036 Silicone/0.002 Teflon LinersW224622-03 Natural PE Cap 100W224622-0303 Red PE Cap 100W224622-0304 Blue PE Cap 100W224622-0305 Yellow PE Cap 100W224622-0307 Green PE Cap 100

12 x 32 mm Amber Vial with Patch and Snap Cap Cat. No. Description Qty/Case





With 0.002 Teflon®/0.038 Red Rubber Septa242786 Natural Cap 100 1000242786-01 Blue Cap 100 1000242786-02 Green Cap 100 1000242786-04 Red Cap 100 1000242786-05 Yellow Cap 100 1000

With 0.002 Teflon®/0.038 Silicone Septa242775 Natural Cap with Slit 100 1000242776 Natural Cap 100 1000242776-01 Blue Cap 100 1000242776-02 Green Cap 100 1000242776-04 Red Cap 100 1000242776-05 Yellow Cap 100 1000

With 0.002 Red Teflon®/0.036 Silicone/0.002 Red Teflon® Septa242772 Natural Cap 100 1000242772-01 Blue Cap 100 1000242772-02 Green Cap 100 1000242772-04 Red Cap 100 1000242772-05 Yellow Cap 100 1000242772-06 Natural Cap with Slit 100 1000

With 0.010 Teflon® Septa242782 Natural Cap 100 1000

Organize your 12 x 32 mm vials in the Wheaton autoclavable polypropylene vial rack (Cat. No. 985800). See page 149 for details.

Crimp or decap your 11 mm aluminum seals with the E-Z Crimper (Cat. No. W225301) and the E-Z Decapper (Cat. No. W225351). See page 96.

Convenience

Packs Convenie

nce

Packs

91


Autosampler Vials

Manufactured from clear Type I borosilicate glassRound shoulder and bottom allows for even heating6 – 20 mL sizeAccepts 20 mm aluminum sealPurchase vials separately or together with seals in Convenience Packs

Specifications:Diameter x Height

6 mL ................................................................................22 x 38 mm10 mL ...............................................................................23 x 46 mm20 mL ..............................................................................23 x 75 mm

Aluminum seal size ............................................................................. 20 mm

Wheaton Headspace Vials feature rounded shoulders and bottoms, allowing for even heating and safer operation at higher pressures. The newly designed top provides a tighter seal with your septa. Vials are manufactured from Type I borosilicate glass and accept 20 mm aluminum seals.

Cat. No Size (mL) Qty/Case

225277 6 100225278 10 100225280 20 100

20 mm crimp closure with liner for headspace vialsScore lines allow for internal pressure releaseChoice of three liner optionsPurchase seals separately or together with Vials in Convenience Packs

These seals are special crimp closures with bridges and scorelines in the aluminum seal. This design is an added safety feature, allowing the internal pressure to be released when 3.0 +/-0.5 bar has been exceeded. The seals come with Teflon® faced silicone, Teflon® faced grey molded butyl, or aluminum faced silicone liners. They are offered in packs of 100 or can be purchased with vials in Convenience Packs.


W224215 20 mm Aluminum Seal with Teflon®/Silicone Septa 100W224216 20 mm Aluminum Seal with Teflon®/Gray Butyl Molded Septa 100W224217 20 mm Aluminum Seal with Aluminum Faced Silicone Septa 100

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Each Convenience Pack includes 100 vials and 100 aluminum sealsChoice of three liners


10 mL Headspace Vial W225278-01 10 mL Vial with 20 mm Aluminum Seal with Teflon®/Silicone Septa 100W225278-02 10 mL Vial with 20 mm Aluminum Seal with Teflon®/Gray Butyl Molded Septa 100W225278-03 10 mL Vial with 20 mm Aluminum Seal with Aluminum Faced Silicone Septa 100

20 mL Headspace Vial W225280-01 20 mL Vial with 20 mm Aluminum Seal with Teflon®/Silicone Septa 100W225280-02 20 mL Vial with 20 mm Aluminum Seal with Teflon®/Gray Butyl Molded Septa 100W225280-03 20 mL Vial with 20 mm Aluminum Seal with Aluminum Faced Silicone Septa 100

Manufactured from clear Type I borosilicate glass20 mL sizeAccepts 20 mm aluminum sealSeal, septa and stopper sold separately

Specifications:Diameter x Height ....................................................................23.5 x 75 mmAluminum seal size ............................................................................ 20 mm

This 20 mL vial fits most headspace analyzers. It is manufactured from Type I borosilicate glass and sold in convenient shrink-wrapped trays. Unlined aluminum seals are available in open top or center disc tear-out styles. Aluminum seals, stopper and septa are sold separately.


985688 20 mL Headspace Vial 960

Unlined Aluminum Seals224178-01 20 mm Open Top 1000224183-01 20 mm Center Disc Tear-Out 1000

Stoppers/Septa224100-181 20 mm Gray Butyl Stopper 1000224173 20 mm Teflon®/Silicone Septa 100224168* 20 mm Teflon® Faced Gray Butyl Septa 100

*This item is a specially molded septa with a Teflon® insert. The sealing surface of butyl and Teflon® effect a more positive seal than plain Teflon® faced septa.

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Available in

Convenience

Packs

Available in

Convenience

Packs

Organize your headspace vials in the Wheaton autoclavable 36-position vial rack (Cat. No. 868805). See page 149 for details.

92Autosampler Vials


12 x 32 LVI Vial & 11 mm Aluminum E-Z Seal™

12 x 32 LVI Vial & 9 mm ABC Screw Cap

LVI Vials and 11 mm Aluminum Seals and/or 9 mm ABC Screw Caps are available together in Convenience Packs of 100. The clear and amber vials with a sealed-in, limited volume 300 μL insert are made of Type I borosilicate glass and have a white ceramic “write-on” patch.

12 x 32 Clear LVI Vial with 11 mm E-Z Aluminum Seal


With 0.002 Teflon®/0.037 Red Rubber Liners W225223-01 Natural Aluminum 100W225223-0103 Red Aluminum 100W225223-0104 Blue Aluminum 100W225223-0107 Green Aluminum 100

With 0.005 Red Teflon®/0.050 Silicone Liners W225223-02 Natural Aluminum 100W225223-0203 Red Aluminum 100W225223-0204 Blue Aluminum 100W225223-0207 Green Aluminum 100

With 0.005 Red Teflon®/0.040 Silicone/0.005 Red Teflon® LinersW225223-03 Natural Aluminum 100W225223-0303 Red Aluminum 100W225223-0304 Blue Aluminum 100W225223-0307 Green Aluminum 100

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12 x 32 Amber LVI Vial with 11 mm E-Z Aluminum Seal



With 0.005 Red Teflon®/0.050 Silicone LinersW225225-02 Natural Aluminum 100W225225-0203 Red Aluminum 100W225225-0204 Blue Aluminum 100W225225-0207 Green Aluminum 100

With 0.005 Red Teflon®/0.050 Silicone/0.005 Red Teflon® LinersW225225-03 Natural Aluminum 100W225225-0303 Red Aluminum 100W225225-0304 Blue Aluminum 100W225225-0307 Green Aluminum 100


12 x 32 Clear LVI Vial with 9 mm ABC Screw Cap


With 0.005 Teflon®/0.035 Red Rubber LinersW225326-01 Natural PP ABC Cap 100W225326-0101 Black PP ABC Cap 100W225326-0103 Red PP ABC Cap 100W225326-0104 Blue PP ABC Cap 100W225326-0105 Yellow PP ABC Cap 100W225326-0107 Green PP ABC Cap 100

With 0.005 Red Teflon®/0.035 Silicone LinersW225326-02 Natural PP ABC Cap 100W225326-0201 Black PP ABC Cap 100W225326-0203 Red PP ABC Cap 100W225326-0204 Blue PP ABC Cap 100W225326-0205 Yellow PP ABC Cap 100W225326-0207 Green PP ABC Cap 100

With 0.005 Red Teflon®/0.035 Silicone/0.005 Teflon® LinersW225326-03 Natural PP ABC Cap 100W225326-0301 Black PP ABC Cap 100

With 0.005 Blue Teflon®/0.035 Silicone LinersW225326-04 Natural PP ABC Cap 100W225326-0401 Black PP ABC Cap 100

With 0.010 Teflon® LinersW225326-06 Natural PP ABC Cap 100W225326-0601 Black PP ABC Cap 100

With 0.005 Red Teflon®/0.035 Silicone Liners with SlitW225326-08 Natural PP ABC Cap 100W225326-0801 Black PP ABC Cap 100

12 x 32 Amber LVI Vial with 9 mm ABC Screw Cap




With 0.005 Red Teflon®/0.035 Silicone/0.005 Teflon® LinersW225328-03 Natural PP ABC Cap 100W225328-0301 Black PP ABC Cap 100




Borosilicate glass vial with 300 μL glass limited volume insertLimited Volume Insert is fused to vial which prevents needle damageWriting patch on all vialsCrimp or screw cap finishPurchase vials and caps separately or together in Convenience Packs

Specifications:Diameter x Height ........................................................................... 12 x 32 mmVolume: Vial with insert .............................................................................300 μLCrimp Seal Size .......................................................................................11 mmABC Screw Cap Size ..................................................................................9 mm

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The LVI Vials are 12 x 32 mm large opening vials with a sealed-in, limited volume insert. The manufacturing process precisely controls the depth and position of the insert relative to the bottom of the vial. This allows more consistent sample recovery from vial to vial and reduces the possibility of autosampler needle damage. The insert has a slight flare at the top, providing a larger needle target area, which prevents needle damage. The LVI Vials have a 300 μL capacity with a residual volume of only 4 μL, depending on the solvent, needle style and depth adjustment of the autosampler. All LVI Vials are supplied with a white ceramic “write-on” patch for easier sample identification and are compatible with all autosamplers that use 12 x 32 mm vials. Inserts and clear vials are manufac-tured from ASTM Type I, Class A borosilicate glass. Amber vials are manufactured from borosilicate glass. The LVI Vials are available with either an 11 mm crimp top finish, which can be used with all standard 11 mm aluminum seals, or with Wheaton’s special 9 mm ABC screw thread. For 11 mm E-Z Seals™ see page 89, and for 9 mm ABC Screw Caps see page 94.


11 mm Crimp Top Vial225220-01 0.3 mL LVI Vial, Clear with Writing Patch 100225221-01 0.3 mL LVI Vial, Amber with Writing Patch 100

9 mm ABC Screw Thread Vial225326 0.3 mL LVI Vial, Clear with Writing Patch 100225328 0.3 mL LVI Vial, Amber with Writing Patch 100

For 11 mm E-Z Seals see page 89, and for 9 mm ABC Screw Caps see page 94.

Available in

Convenience

Packs

Available in

Convenience

Packs

93


Autosampler Vials

Manufactured from polypropylene300 μL conical interior reduces sample sizeAvailable with snap/crimp or screw top finish100 vials per shelf pack/1000 vials per case

Specifications:Diameter x Height .......................................................................12 x 32 mmVial Volume: ....................................................................................... 300 μLSnap/Crimp Seal Size ........................................................................ 11 mmABC Screw Cap Size ............................................................................ 9 mmTraditional Screw Cap Size ..................................................................8-425

Wheaton 12 x 32 mm polypropylene vials can be used in most standard gas and liquid chromatography autosamplers. These lightweight, economical vials are made from durable polypropylene which has good chemical resistance. The conical interior ensures maximum retrieval of contents without the hassle of using removable inserts. Vials are available with snap/crimp, ABC screw cap and traditional screw cap finish.

Cat. No Closure Style Closure Size For Closures See Shelf Pack Qty/Case

Polypropylene Vial225180 Snap/Crimp Finish 11 mm Page 89-90 100 1000W225181 ABC Screw Top Cap 9 mm Page 94 100 1000225185 Screw Top Cap 8-425 Page 95 100 1000

Thermoplastic polymer outer shell0.1 mL Type I borosilicate glass insertAvailable with snap/crimp or screw top finish100 vials per case

Specifications:Diameter x Height .......................................................................12 x 32 mmVial Volume: ....................................................................................... 100 μLSnap/Crimp Seal Size ........................................................................ 11 mmTraditional Screw Cap Size ...................................................................8-425

Wheaton 0.1 mL plastic autosampler vials, with glass inner cone, combine Type I borosilicate glass and thermoplastic polymer outer vial shells. The glass is the only contact between your sample and the seal. The thermoplastic polymer provides not only excellent dimensional tolerance reliability for capping and instrumentation use, but safety from breakage as well. Vials are available with snap/crimp top or traditional screw cap finish.

Cat. No Closure Style Closure Size For Closures See Qty/Case

Clear Vial225190 Snap/Crimp Finish 11 mm Page 89-90 100225195 Screw Top Finish 8-425 Page 95 100

Amber Vial225200 Snap/Crimp Finish 11 mm Page 89-90 100225205 Screw Top Finish 8-425 Page 95 100

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Manufactured from polypropylene500 or 750 μL conical interior reduces sample sizeAvailable with snap/crimp or screw top finish100 vials per shelf pack/1000 vials per case

Specifications:Diameter x Height .......................................................................12 x 32 mmVial Volume ............................................................................. 500 or 750 μLSnap/Crimp Seal Size ........................................................................ 11 mmTraditional Screw Cap Size ................................................................10-425

The Wheaton polypropylene snap cap vial uses either aluminum crimp seals or plastic snap cap closures, which eliminate the need for crimping and decapping tools, and provide a larger target area. The unique cone-shaped bottom ensures maximum sample retrieval. The snap/crimp top vial uses 11 mm seals. The traditional screw thread vials accept 10-425 screw thread caps.

Cat. No Closure Style Closure Size For Closures See Shelf/Pack Qty/Case

Vial with 500 μL Conical InteriorW225186 Screw Thread Finish 10-425 Page 96 100 1000W225187 Snap/Crimp Finish 11 mm Page 89-90 100 1000

Vial with 750 μL Conical Interior225235 Snap/Crimp Finish 11 mm Page 89-90 100 1000W225237 Screw Thread Finish 10-425 Page 96 100 1000

12 x 32 mm glass vial with conical bottomIdeal for use in autosamplersScrew cap or crimp cap finishSee closures on pages 89 and 94

This High Recovery Vial features a one piece glass design with a conical bottom for complete sample recovery. Reactions and concentrations can be performed directly in the sampling vial, eliminating the need for sample transfer. Use the 9 mm ABC Screw Caps found on page 94 with the screw thread vial, and use the 11 mm Aluminum E-Z Seals™ on page 89 with the crimp top vial.


W225149 1.5 mL, 9 mm ABC Screw Thread Finish 100W225171 1.5 mL, 11 mm Crimp Top Finish 100

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500 μL Vial 500 μL Vial 750 μL Vial 750 μL Vial with Screw Cap Finish with Snap Cap Finish with Screw Cap with Snap Cap Finish

94Autosampler Vials


ABC Vials & Caps Limited Volume Insert

40% larger opening than standard vialsManufactured from clear and amber Type I borosilicate glassLimited volume inserts9 mm screw threadUse with Wheaton ABC Screw Caps

Wheaton ABC Vials have a 40% wider opening than standard screw top vials as well as superior thread design that allows for a more secure seal to the closure. The closures for the ABC Vials feature the Wheaton “Super Septa”, a low extractable septa that is optimal for GC/MS. ABC Vials have a capacity of 1.8 mL and are manufactured from Type I borosilicate glass. Vials are packaged 100 per shelf pack and 10 packs per case.

Specifications:Diameter x Height ........................................................................12 x 32 mmVial volume ........................................................................................ 1.8 mLLimited volume insert ................................................... 0.10, 0.25 & 0.35 mLABC cap size ......................................................................................... 9 mm


ABC VialW225149 1.5 mL, Clear — 100W225150 1.8 mL, Clear 100 1000W225151 1.8 mL, Clear with Writing Patch 100 1000W225152 1.8 mL, Amber 100 1000W225153 1.8 mL, Amber with Writing Patch 100 1000

Limited Volume InsertsW225171 1.5 mL, Clear with Aluminum Seal Finish — 100225255 0.10 mL Glass Insert with Top Spring 100 200225265 0.25 mL Glass Insert with Bottom Spring — 100225257 0.25 mL Polypropylene Insert with Bottom Spring — 100225350-631 0.35 mL Glass Flat Bottom Insert 200 1000

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Manufactured from PolypropyleneLow extractable septa100/pack with 10 packs/case

The closures for the ABC Vials feature the Wheaton “Super Septa”, low extract-able septa that is optimal for GC/MS. Screw caps are available in natural, black, red, blue, yellow or green polypropylene. The screw caps are packaged 100 per shelf pack, 1000 per case.


With 0.005 Teflon®/0.035 Red Rubber LinerW225331-01 Natural PP ABC Cap 100 1000W225331-0101 Black PP ABC Cap 100 1000W225331-0103 Red PP ABC Cap 100 1000W225331-0104 Blue PP ABC Cap 100 1000W225331-0105 Yellow PP ABC Cap 100 1000W225331-0107 Green PP ABC Cap 100 1000

With 0.005 Teflon®/0.035 Silicone LinerW225332-02 Natural PP ABC Cap 100 1000W225332-0201 Black PP ABC Cap 100 1000W225332-0203 Red PP ABC Cap 100 1000W225332-0204 Blue PP ABC Cap 100 1000W225332-0205 Yellow PP ABC Cap 100 1000W225332-0207 Green PP ABC Cap 100 1000

With 0.005 Teflon®/0.035 Silicone/0.005 Teflon LinerW225333-03 Natural PP ABC Cap 100 1000W225333-0301 Black PP ABC Cap 100 1000

With 0.005 Blue Teflon®/0.035 Silicone LinerW225334A-04 Natural PP ABC Cap 100 1000W225334A-0401 Black PP ABC Cap 100 1000

With 0.005 Red Teflon®/0.035 Silicone Liner with SlitW225338-08 Natural PP ABC Cap 100 1000W225338-0801 Black PP ABC Cap 100 1000

With 0.010 Teflon® LinerW225336-06 Natural PP ABC Cap 100 1000W225336-0601 Black PP ABC Cap 100 1000

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Available in

Convenience

Packs

Available in

Convenience

Packs

ABC Vial Convenience Packs are continued on page 95.

ABC Vials and 9 mm ABC Screw Caps are available together in convenience packs of 100. The clear and amber vials are made of Type I borosilicate glass. The 1.8 ml vials are packaged in a recyclable PETE clamshell case to help prevent particulate contamina-tion. The ABC Screw Caps are available in six colors.

12 x 32 Clear ABC Vials & 9 mm ABC Screw Caps




12 x 32 Clear ABC Vials & 9 mm ABC Screw Caps


With 0.005 Red Teflon®/0.035 Silicone/0.005 Red Teflon® LinersW225150-03 Natural PP ABC Cap 100W225150-0301 Black PP ABC Cap 100

With 0.005 Blue Teflon®/0.035 Silicone Liners W225154-04 Natural PP ABC Cap 100W225154-0401 Black PP ABC Cap 100



95


Autosampler Vials

Manufactured from clear and amber Type I borosilicate glass

Limited volume insertsPre-assembled black polypropylene

screw cap with septaScrew cap and septa available

separatelyPurchase vials and caps separately or together in Convenience Packs

These glass vials are the original design used in most autosamplers requiring traditional screw top vials. The vials are manufactured from Type I borosilicate glass. Vials are packaged 100 per shelf pack and 10 packs per case. Glass limited volume inserts are available with flat bottoms or springs. The black polypropylene screw caps can be purchased separately or preassembled. For quick and easy preparation, purchase vials and caps together in Wheaton Convenience Packs.

Specifications:Diameter x Height ........................................................................12 x 32 mmVial volume ........................................................................................ 1.8 mLLimited volume insert ........................................................... 0.10 & 0.25 mLScrew cap size .....................................................................................8-425

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Cat. No. Description Cap Size Shelf Pack Qty/Case

12 x 32 mm Screw Top VialsW225900 1.8 mL, Clear 8-425 100 1000W225910 1.8 mL, Amber 8-425 100 1000


Limited Volume Inserts 225260 0.10 mL Glass Insert with Bottom Spring — 100225350-531 0.25 mL Glass Flat Bottom Insert 200 1000

Preassembled Open Top Caps and Septa240602 8-425 Black Polypropylene Cap with Teflon®/Silicone Liner — 100242245 8-425 Black Top Seal Cap with Natural Polypropylene, 10 mil Septa 100 1000

Open Top CapsW240506 8-425 Black Phenolic Cap — 200W240507 8-425 Black Phenolic Cap (Shimadzu) — 200

SeptaW240580 8 mm Red Teflon® Faced Silicone — 100W240581 8 mm Teflon® Faced Silicone — 100

Convenience Packs225170 1.8 mL Clear Vial with Unassembled Cap and Septa 240224950 1.8 mL Clear Vial in Vial File with Preassembled Cap and Septa 60

Standard Screw Top Vial Limited Volume Insert

Available in

Convenience

Packs

Available in

Convenience

Packs

12 x 32 Clear ABC Vial with Patch & 9 mm ABC Screw Cap




With 0.005 Red Teflon® 0.035 Silicone/ /0.005 Red Teflon® LinersW225151-03 Natural PP ABC Cap 100W225151-0301 Black PP ABC Cap 100




12 x 32 Amber ABC Vial with 9 mm ABC Screw Cap




With 0.005 Red Teflon®/0.035 Silicone /0.005 Red Teflon® LinersW225152-03 Natural PP ABC Cap 100W225152-0301 Black PP ABC Cap 100




12 x 32 Amber ABC Vial with Patch & 9 mm ABC Screw Cap




With 0.005 Red Teflon®/0.035 Silicone /0.005 Red Teflon® LinersW225153-03 Natural PP ABC Cap 100W225153-0301 Black PP ABC Cap 100




96Autosampler Vials


Manufactured from clear and amber Type I borosilicate glassLimited volume insertsPre-assembled black polypropylene screw cap with septaPurchase vials and caps separately

Wheaton Screw Top E-Z Vials with Step, feature a large opening with a long neck for greater sample accessibility with reduced needle damage. Vial capacity has been increased to 1.8 mL. The innovative design of the vial and limited volume insert enables the insert to be precisely centered inside the neck of the vial. The Screw Top E-Z Vials with Step are designed to use 10-425 screw caps. Vials are manufactured from Type I borosilicate glass and are packaged in shrink-wrapped trays of 100 for convenience and cleanliness. Screw caps and septa available separately.

Specifications:Diameter x Height ........................................................................12 x 32 mmVial volume ........................................................................................ 1.8 mLLimited volume insert ...................................................................... 0.25 mLScrew cap size ....................................................................................10-425


12 x 32 Screw Top E-Z Vials with Step224626 1.8 mL with Step, Clear 100 1000224627 1.8 mL with Step, Clear with Writing Patch 100 1000224628 1.8 mL with Step, Amber 100 1000224629 1.8 mL with Step, Amber with Writing Patch 100 1000


Limited Volume Inserts225258 0.25 mL Glass Insert for Vial with Step 100225259 0.25 mL Polypropylene Insert for Vial with Step 100

Preassembled Open Top Caps and Septa242760 10-425 Black Polypropylene Cap with Teflon® Red Rubber 100242761 10-425 Black Polypropylene Cap with Teflon®/Silicone/Teflon® 100242762 10-425 Black Polypropylene Cap with Teflon®/Silicone 100242766 10-425 Top Seal with Natural Polypropylene, 10 mil Septa 1000

Solid Top Caps 242765 10-425 Black Polypropylene Unlined Cap 1000

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Manufactured from clear and amber Type I borosilicate glassLimited volume insertsPre-assembled black polypropylene screw cap with septaPurchase vials and caps separately

Specifications:Diameter x Height ........................................................................15 x 45 mmVial volume ......................................................................................... 4.0 mLLimited volume insert ...................................................................... 0.30 mLScrew cap size ....................................................................................13-425

Wheaton 15 x 45 Screw Top Vials are designed primarily for use in the Waters WISP® 48-position and other autosamplers. The vials are manufactured from Type I borosilicate glass and packaged in shrink-wrapped trays of 100 for convenience and cleanliness. Screw caps and septa available separately.


15 x 45 mm Screw Top Vials224794 4.0 mL, Clear 100 1000224794-01 4.0 mL, Clear with Writing Patch 100 1000224795 4.0 mL, Amber 100 1000224795-01 4.0 mL, Amber with Writing Patch 100 1000


Limited Volume Inserts225268 0.30 mL Glass Insert with Bottom Spring 100

Preassembled Open Top Caps and Septa242768 13-425 Black Polypropylene Cap with PTFE/Silicone Liner 100242247 13-425 Top Seal Cap with Natural Polypropylene, 10 mil Septa 1000

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Crimps aluminum seals to autosampler vials

Cushioned ergonomic handleLabeled with size for quick size

identificationPolished crimping jaws for

consistent sealing

For more details on this product see page 35


W225300 E-Z Crimper, 8 mm 1W225301 E-Z Crimper, 11 mm 1W225302 E-Z Crimper, 13 mm 1W225303 E-Z Crimper, 20 mm 1

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• Removes aluminum seals from autosampler vialsCushioned ergonomic handleLabeled with size for quick size identification

For more details on this product see page 35


W225350 Decapper, 8 mm 1W225351 Decapper, 11 mm 1W225352 Decapper, 13 mm 1W225353 Decapper, 20 mm 1

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97


LC & TLC

Manufactured from Type I borosilicate glass13-425 screw threads on both ends50 and 100 mm lengthSuitable for aqueous or organic applications

These general purpose columns are suitable for a wide variety of separations using either aqueous or organic solvents. They are manufactured from Type I, Class A borosilicate glass.

Cat. No. ID x Length (mm) Screw Thread Size Qty/Case

265318 8 x 50 13-425 1265320 8 x 100 13-425 1

Manufactured from Type I borosilicate glassIncludes glass coverAutoclavable

The Wheaton Mini-TLC is useful for developing pre-scored or smaller-size TLC plates. This chamber is ideal for pilot studies, monitoring organic reactions and determining mobile phase composition in HPLC separations. It is engineered to reduce the consumption of solvent necessary to saturate the chamber. The Mini-TLC Chamber

is manufactured from Type I, Class A borosilicate glass.

Cat. No. Dia x H (mm) Qty/Case

276845 60 x 105 6276850 60 x 205 3

Holds plates up to 20 x 20 cm in sizeMade from soda-lime glassGround glass cover securely fits

chamber to reduce solvent evaporationAutoclavable

This sturdy chamber accommodates standard 20 x 20 cm thin layer chromatography plates. The top surface is ground to a precise tolerance to provide a secure sealing area and to ensure both chamber saturation and optimum plate development. It comes complete with a glass lid.

Cat. No. Internal Dimensions, L x W x H (cm) Qty/Case

276860 27 x 7 x 24 1

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Manufactured from soda-lime glassHolds 5 small TLC platesIncludes glass lid

The staining jar is designed with an extra tall lid to accept five single 25 x 75 mm to 25 x 100 mm slides or 10 slides back to back

Cat. No. Internal Dimensions, L x W x H (mm) Qty/Case

276840 26 x 26 x 90 6

Made from soda-lime glassHolds 8 small TLC platesIncludes glass lid

This Hellendahl-type dish can be used for staining or as a TLC developing chamber. It holds eight individual 3” x 1” (75 mm x 25 mm) slides or, if necessary, 16 slides back-to-back.

Cat. No. Internal Dimensions, L x W x H (mm) Qty/Case

900630 76 x 25 x 75 6

Adjustable lengthGrease-free Wheaton Connection®

This convenient, pencil-like device is designed for the removal of sample from a developed TLC plate by vacuum. The sample is trapped on the sealed-in frit and eluted with solvent for sample analyte recovery. All wetted parts are Wheaton 33 low extractable borosilicate glass (conforms ASTM Type I, Class A and USP Type I requirements) or Teflon®. All screw threads are 13-425 size. The length of the recovery tube is adjustable. The approximate pore size of the fritted funnel is 40 to 60 microns.


277930 TLC Sample Recovery Tube 1277935 Replacement Glass Tip 2

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98


Atomizes chromagenic reagentsFinger vent provides spray controlAvailable with 125 or 250 mL reservoir Wheaton Standard Taper 19/22 Clear-Seal® Joint

The TLC Reagent Sprayer atomizes chromagenic reagents into a very fine mist, making it ideal for spraying reagents on TLC and paper chromatograms. These sprayers come with a convenient finger vent for instant spray control. Requires 10 psi air pressure.

Cat. No. Description Size (mL) Qty/Case

279040 Complete Unit 125 1279045 Sprayer Head Only 125 1635920 Flask Only 125 1279041 Complete Unit 250 1635921 Flask Only 250 1

Atomizes chromagenic reagentsFinger vent provides spray control65 mL reservoir

This economical chromatographic sprayer produces the very fine uniform mist (with no droplets) that is required for TLC and paper chromatography. Made from Type I borosilicate glass, it features a convenient finger vent for instant spray control. Requires 10 psi air pressure.

Cat. No. Size (mL) Qty/Case

279050 65 1

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•••

For a complete list of aluminum seals, rubber stoppers, septa and screw caps, see the Cap and Closure Section on pages 31-42.

Conical well design with exacting dimensions ensures maximum recovery of high value samples

Manufactured to extremely tight tolerances for reliable operation in automated sample handling and storage systems

SciLutions® customized services including bar coding, critical cleaning, surface treatment, and taring

Custom vial configurations available

Ideal for compound management applications, the new Wheaton E-Z Ex-Traction Vials® are available in 2, 4, 8,10, and 20 mL sizes. Also available in amber glass upon request.

For more information see page 143 or visit our web site www.wheatonvial.com.

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E-Z Ex-TractionHigh Recovery

Vials

®

99


Bottle Top

Friendly digital volume setting

Fast and precise volume adjustment. Efficient click-stop mechanism prevents unwanted altera-tion. Large display is easy to read; the window adjusts to desired side of instrument body.

Optimized working position

The instrument rotates 360° for adequate positioning on top of the bottle.

In-lab calibration

Calibrex™ dispensers are factory calibrated and can be easily recalibrated by means of a dedicated adjustment screw.

W heaton/Socorex Bottle Top Dispensers have a sophisticated yet simple design that provides easy operation, superior safety, and low maintenance. They are accurate enough to

use in research yet rugged enough to use in student labs. Our bottle top dispensers provide the following features:

Solid Calibration® provides consistent volumes now and in the future

High chemical resistance- use with a wide range of liquids

Easily calibrated in your lab

User-friendly volume selection

Wide range of accessories

The dispenser delivers accuracy and long term reproducibility through Solid Calibration®. A series of calibrated permanent fixed stops make volume selection quick and repeatable, not dependent on operator skill. The wetted components are chemically inert with parts made of materials such as platinum-iridium, ceramics, borosilicate glass, and modern fluoropolymers. Our bottle top dispensers work well with acids, bases, and organic solvents. See the chemical resistance chart on page 102.

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Superior chemical resistanceLong-lasting performance stability Simple construction – no tool for

disassembling Instant volume settingIn-lab calibrationAutoclavable at 121°C/250°F fully

assembledOne year warranty

Designed for performance handling of liquids from a large variety of bottles and flasks, the Calibrex™ dispensers combine the latest in dosing technology, high-tech materials and ergonomic design. Manufactured and tested to fully comply with current regulations. Three color-coded 520 models with adjustable volumes ranging from 0.25 – 2 mL, 1 – 5 mL and 1 – 10 mL. The Calibrex has a 32 mm screw thread and three adapters are included with the unit (28, 38, and 45 mm) and a 300 mm feed tube.

Color Inaccuracy (E%) Imprecision (CV%) Cat. No. Code Volume Division Min. Vol Mid. Vol. Max. Vol. Min. Vol Mid. Vol. Max. Vol. Qty/Case

844000 Green 0.25 – 2 mL 0.05 mL < ± 3.0% < ± 1.8% < ± 0.6% < 0.5% < 0.35% < 0.1% 1844002 Yellow 1 – 5 mL 0.1 mL < ± 2.0% < ± 1.3% < ± 0.6% < 0.5% < 0.35% < 0.1% 1844004 Blue 1 – 10 mL 0.25 mL < ± 1.5% < ± 1.1% < ± 0.6% < 0.5% < 0.35% < 0.1% 1

Performance values obtained with double distilled water at constant temperature (± 0.5°C) comprised between 20 and 25°C in accordance with EN ISO 8655. Each dispenser has a 32 mm screw thread and included with each unit are three adapters (28, 38, 45 mm) and a 300 mm feed tube.

See page 102 for chemical compatibility chart.

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Superior Material Selection Parts Material

Feed Tube PTFE Intake Valve Ceramic Valve Balls Pyrex® Glass Valve Spring Platinum-iridium Barrel Borosilicate Glass Barrel Plate PTFE Plunger Glass with PFA coating

Outlet Valve Ceramic Body ETFE Delivery jet assembly PTFE/ETFE

Parts coming in contact with the liquid flow are chemically inert.

100Bottle Top


A true digital system

Cylindrical cam with pre-calibrated steps – one for each volume – contributes to the long-term calibration stability.

In-lab calibration

The Calibrex™ dispensers are factory calibrated. Recalibration easily performed by means of a dedicated adjustment screw.

Integrated dosing mechanismSimple, robust construction Retractable graduated column reduces instrument height Fluid path materials excluding any metalChoice of reservoir sizes, amber glass and clear borosilicate

glassFully autoclavable at 121°C/250°FOne year warranty

Compact dispensers for safe reagent handling, adapted to refrigerator storage as well as water bath heating. Dosing mechanism entirely protected inside reservoir. Manufactured and tested to fully comply with the latest regulations on instrument safety and precision. Volumes range from 0.2 to 30 mL. The Acurex 501 comes with an amber glass reservoir, and the 502 model comes with a borosilicate glass reservoir.

501 Amber 502 Borosilicate Reservoir Imprecision (CV%)Cat. No. Cat. No. Volume Division Capacity (mL) Min. Vol Mid. Vol. Max. Vol. Qty/Case

844060 — 0.2 – 2 mL 0.1 mL 250 < 0.5% < 0.35% < 0.1% 1W844055 W844061 0.2 – 2 mL 0.1 mL 1000 < 0.5% < 0.35% < 0.1% 1W844056 — 0.2 – 2 mL 0.1 mL 2000 < 0.5% < 0.35% < 0.1% 1844062 W844063 0.4 – 5 mL 0.2 mL 500 < 0.5% 1) < 0.35% < 0.1% 1W844057 W844065 0.4 – 5 mL 0.2 mL 1000 < 0.5% 1) < 0.35% < 0.1% 1W844058 W844066 0.4 – 5 mL 0.2 mL 2000 < 0.5% 1) < 0.35% < 0.1% 1844064 — 1 – 10 mL 0.2 mL 1000 < 0.5% < 0.35% < 0.1% 1W844059 W844067 1 – 10 mL 0.2 mL 2000 < 0.5% < 0.35% < 0.1% 1844068 — 1 – 30 mL 1.0 mL 2000 < 0.5% 2) < 0.35% < 0.1% 1

Performance values obtained with double distilled water at constant temperature (± 0.5°C) comprised between 20 and 25°C in accordance with EN ISO 8655. 1) measured at 0.5 mL 2) measured at 3 mL

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Superior chemical resistanceLong-lasting performance stabilityComfortable and convenient in useSolid, yet simple constructionInstant volume settingIn-lab calibrationAutoclavable at 121°C/250°F fully assembledOne year warranty

Three 521 sizes expand the Calibrex™ line with larger dispensing volumes ranging from 2 to 20 mL, 10 to 50 mL and 10 to 100 mL. Similar to the 520 model in many respects, such as construction quality, sturdiness, chemical resistance, smooth plunger travel, in-lab calibration, autoclaving, the 521 model has a distinct black design. Also fitted with the Solid Calibration adjustment system, the Calibrex™ 521 has an additional fine-tuning ring. Each dispenser has a 32 mm screw thread and included with each unit are three adapters (38, 40, 45 mm) and a 300 mm feed tube (375 mm for Cat. No. 844023).

Inaccuracy (E%) Imprecision (CV%) Cat. No. Volume Division Min. Vol Mid. Vol. Max. Vol. Min. Vol Mid. Vol. Max. Vol. Qty/Case

844021 2 – 20 mL 0.1 < ± 1.5% < ± 1.1% < ± 0.6% < 0.5% < 0.35% < 0.1% 1844022 10 – 50 mL 1.0 < ± 1.5% < ± 1.1% < ± 0.6% < 0.5% < 0.35% < 0.1% 1844023 10 – 100 mL 1.0 < ± 1.5% < ± 1.1% < ± 0.6% < 0.5% < 0.35% < 0.1% 1

Performance values obtained with double distilled water at constant temperature (± 0.5°C) comprised between 20 and 25°C in accordance with EN ISO 8655. Each dispenser has a 32 mm screw thread and included with each unit are three adapters (38, 40, 45 mm) and a 300 mm feed tube (375 mm for Cat. No. 844023).

See page 102 for chemical compatibility chart.

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Space saving low profile

The graduated column is retractable for height reduction, making the dispenser ideal for storage in refrigerators.

Chemically inert materials

All parts in contact with the liquid are made from chemically inert materials:

Parts Materials

Valve Pyrex® glass and synthetic ruby Barrel Neutral glass Plunger PTFE-coated glass Reservoir Amber glass or Borosilicate glass (resistant > 250°C/482°F) Delivery jet and union PVDF/FEP/PFA

Superior Material Selection Parts Material

Feed Tube PTFE Intake Valve Borosilicate Glass Valve Balls Ceramic, Ruby Valve Spring Platinum-iridium Barrel Borosilicate Glass Barrel Plate PTFE Plunger Glass with PFA coating Outlet Valve Ceramic Body ETFE Delivery jet assembly PTFE/ETFE

Parts coming in contact with the liquid flow are chemically inert.

101


Bottle Top

For the Calibrex 520 and 521 DispensersMade from PP (polypropylene) or PTFE

Both the Calibrex 520 and 521 Bottle Top Dispensers come standard with a 32 mm screw thread, and included with each dispenser are three polypropylene bottle neck adapters (28, 40, and 45 mm for the 520 model; and 38, 40, 45 mm for the 521 model). If replacement or different size

adapters in different materials are needed they can be purchased separately.

Cat. No. Screw Thread Size (mm) Qty/Case

Threaded, PP W830000 22 1W830002 25 1844032 28 1W830004 30 1W830006 34 1W830008 36 1844035 38 1W830010 40 1844038 45 1

Threaded, PTFE W830012 36 1W830014 38 1W830016 40 1W830018 45 1

Cat. No. Taper Qty/Case

Tapered, PP W830020 19/26 1W830022 24/29 1W830024 29/32 1W830026 45/40 1

Tapered, PTFE W830029 19/26 1W830031 24/29 1W830032 29/32 1

Autoclavable PTFE delivery Jet-Pen™ and extension tubing help dispense into vessels with maximum comfort within a 60 cm distance. Fits Calibrex™ 520/521 dispensers up to 20 mL.

Replacement Feed Tubing

Each Calibrex™ digital dispenser is supplied with one PTFE feed tubing. Spare feed tubings are available to a standard size. Uncut by the meter is available by request. The latter is recommended when aspirating from remote bulk containers.

Cat. No Description Length Inside Dia.

Extension Tubing844024 Extension tubing for dispensers up to 20 mL 600 mm 2.2 mm

Replacement Feed TubingI051154 Replacement feed tubing, 520, 2, 5, 10 mL 300 mm 5 mmI050916 Replacement feed tubing, 521, 20 mL 300 mm 7.5 mmI050917 Replacement feed tubing, 521, 50 mL 300 mm 10 mmI050349 Replacement feed tubing, 521, 100 mL 375 mm 10 mm

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Clear bottles are made from borosilicate glass

Amber bottles are square, round or with jug handle

Amber glass bottles are available safety coated

Square polyethylene bottles are available in 250 – 2500 mL size

This large selection of reagent bottles is supplied with screw caps and compatible with Socorex Calibrex 520 and

521 Dispensers. The different sized screw thread adapters that correspond to these bottles are supplied with each dispenser. Adapters can also be purchased separately. See the Adapters on this page.

Cat No. Shape Volume Screw Thread Size Qty/Case

Amber GlassW832000 Square 100 mL 32 mm 1844025 Square 250 mL 32 mm 1844027 Square 500 mL 32 mm 1844029 Square 1000 mL 45 mm 1844030 Round 2500 mL 45 mm 1

Amber Glass, with HandleW832004 Round 2500 mL 45 mm 1

Polyethylene Coated Amber Glass W832006 Square 1000 mL 45 mm 1W832008 Round 2500 mL 45 mm 1

Clear Borosilicate GlassW832010 Round 250 mL 45 mm 1W832012 Round 500 mL 45 mm 1W832014 Round 1000 mL 45 mm 1W832016 Round 2000 mL 45 mm 1

Clear Pyrex® Glass, with Connection Neck844026 Round 500 mL 32 mm 1

Polyethylene Bottle W832018 Square 250 mL 25 mm 1W832020 Square 500 mL 25 mm 1W832022 Square 1000 mL 32 mm 1W832024 Square 2500 mL 45 mm 1

Stabilizes dispenser creating a safe environmentFacilitates liquid intake from a remote container

The Dispenser Stand holds dispensers up to 50 mL when screwed on small size bottles. For bulk container aspiration, the Stand facilitates remote liquid intake dispensing up to 50 mL or 100 mL (< 10 m distance, < 2 m elevation). The dispenser and feed tube can be ordered separately.

Cat. No. Description Qty/Pk

W832026 Stand for dispensers up to 50 mL 1W832028 Stand for remote liquid intake, dispensers up to 50 mL* 1W832030 Stand for remote liquid intake, dispensers 100 mL* 1

* Dispenser and feed tube not included, to be ordered separately

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102Bottle Top


The following list includes most currently used chemicals. It provides useful information for the safe and adequate use of Acurex™ 501 and Calibrex™ 520/521 dispensers. However, safety precautions and recommendations in operating instructions must be followed carefully.

A = Good resistanceB = Acceptable with limitationsC = Not recommended

1 = Possible crystallization – blockage (do not let dry plunger/barrel together).2 = Swell of plunger protection layer, possible peeling.3 = Acid vapors (better resistance with lower concentration). Do not leave instrument on bottle.4 = Risk of softening or discoloration of external parts through vapors. Do not leave instrument on bottle.5 = Chemical degradation of glass parts (plunger/barrel).

Reagents Acura Calibrex 501 520 521

Acetic acid 100% A A B/1Acetic acid diluted A A B/3Acetone A B/4 AAcetonitrile A A AAscorbic acid A A ABenzaldehyde A A ABenzene A B/4 B/4Boric acid A A ABromine B/2 B/2 C/2/4Butanol A A AButanone A B/4 ACarbon disulfide A A AChloroform A B/4 B/4Chloronitric acid 100% B/2/3 B/3 C/3Chlorosulfuric acid 100% B/2/3 B/3 B/3Chromic acid 100% B/2/3 B/3 B/3Citric acid A A ACyclohexane A A ACyclohexanone A A ADiethylene glycol A A ADiethylether A A ADimethylformamide (DMF) A B/4 ADimethylsulfoxide (DMSO) A A ADioxane / Diethylene dioxide A A B/4


Ethanol A A AEther A B/4 B/4Ethyl acetate A A B/4Ethylene glycol A A AFormaldehyde (Formalin) A A AFormic acid A A AGasoline A A AGlycerin < 40% A A AHexane A A AHydrochloric acid 100% B/2/3 A B/3Hydrofluoric acid 100% (HF) C/5 C/5 C/5Hydrogen peroxide A A AIodine A A AIsopropanol A A ALactic acid A A ALiquid ammonia A A AMethanol A A AMethyl ethyl ketone (MEK) A B/4 AMethylmethacrylate (MMA) A A AMineral oil A A ANitric acid 100% B/2/3 B/3 C/3Nitric acid dil. < 30% A A AOxalic acid A A APerchloric acid 100% B/2/3 B/3 B/3


Perchloric acid diluted A A APetrol benzene A A B/4Petroleum ether A A B/4Phenol A A APhosphoric acid 100% A A APotassium hydroxide B/1 B/1 B/1Potassium permanganate A A APropronic acid A A ASodium chloride / cooking salt B/1 A ASodium hydroxide B/1 B/1 B/1Sodium hypochloride A A ASulfochromic acid 100% B/2/3 B/2/3 B/2/3Sulfonitric acid 100% B/2/3 B/2/3 B/2/3Sulfuric acid 100% B/2/3 B/2 B/2Tetrahydrofuran (THF) B/2/4 B/4/2 B/2/4Toluene A B/4 B/4Trichlorethylene B/4 B/4 B/4Trichloroacetic acid A A ATrichloroethane/methane B/4 B/4 B/4Trichlortrifluoroethane B/4 B/4 B/4Veg. / anim. / min. oil A A AXylene A B/4 B/4

Disassembling/reassembling is facilitated by the limited number of well connected parts - no tools needed.

For automatic dispensing, see the Wheaton Omnispense Plus® peristaltic pump that can operate as a constant rate flow pump or as a programmed dose dispenser.

For more details see page 126.

103


Self-Refilling Syringe

Simple, natural movements

Both pistol-grip and two-ring handles fit any hand in a comfortable way. Activation performed with limited effort for efficient work even during long dispensing series.

Volume selection – Reliable reproducibility

Volume is set within seconds. It will not vary while activating the syringe, thanks to the safety-lock-ing nut on the micrometric screw. Dose-by-dose reproducibility (CV) is excellent, as witnessed by performance figures.

Plunger tightness without O-ring

Smooth plunger travel and tightness achieved through high-precision stainless steel plunger and grounded glass barrel. No O-ring to mess with or replace.

Optimal hand balance and ergonomicsFast and reliable volume setting Excellent reproducibilityPlunger tightness without O-ringHigh chemical resistanceFully autoclavable at 121°C/250°FOne year warranty

From the basic unit to the corrosion-resistant model, the laboratory dedicated syringe line offers precision instruments you can trust. Intended for safe and reliable liquid distribution, the selection of pistol-grip and two-ring instruments comes in a wide volume range.

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Fill-by-fill (automatic) through needle or cannulaSupplied without valve systemLuer Lock nozzleTwo-ring and pistol-grip handle

Package includes syringe, PVC protection sleeve and operating instructions.

Self-refilling automaticIntegrated valve systemLuer Lock nozzleTwo-ring and pistol-grip handle

Package includes syringe, one meter silicone feed tube, sinker, PVC protection sleeve, aspiration and vent cannulas, set of spare for valve, operating instructions.

Self-refilling automaticDetachable valve systemEnhanced corrosion resistanceLuer Lock nozzleTwo-ring and pistol grip handle

Package includes syringe, one meter silicone feed tube, sinker, PVC protection sleeve, aspiration, vent and dosing cannulas, set of spare for valve, operating instructions.

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104Self-Refilling Syringe


Dose-by-dose distribution

Dispensing distribution

Filtration at your nozzle tip

Animal injection

Sterile work

Aspiration with reverse modus valve

(for Chemical Resistance)

Parts Basic/classic models Premium models

Feed tubing Silicone Silicone Barrel Borosilicate glass Borosilicate glass Washer, Barrel FPM FPM Plunger Stainless steel DIN 304 Stainless steel DIN 316L Valve system Ni-Cr Brass Stainless steel DIN 316L Washer, valve PTFE PTFE Spring and balls, valve Stainless steel, DIN 304 Stainless steel, DIN 304 Sinker, feed cannula Ni-Cr Brass Ni-Cr Brass

Few parts assembly – Low maintenance

The automatic syringes are made of only six main parts, easy to disassemble. Fast access to all elements allows rapid and efficient cleaning. Spares are available at reasonable cost.

Imprecision (CV%) Cat. No. Volume (mL) Divisions (mL) Min.Vol. Mid.Vol. Max.Vol. Qty/Case

Dosys basic syringe 162 with Two-ring HandleW852000 0.1 – 1 0.05 < 0.7% < 0.6% < 0.4% 1W852002 0.3 – 2 0.1 < 0.6% < 0.5% < 0.4% 1W852004 0.5 – 5 0.5 < 0.5% < 0.4% < 0.2% 1

Dosys classic syringe 163 with Two-ring HandleW852006 0.025 – 0.3 0.025 < 1.8% 1) < 1.2% < 0.5% 1W852008 0.1 – 0.5 0.1 < 1.2% < 1.9% < 0.4% 1W852010 0.1 – 1 0.05 < 0.7% < 0.6% < 0.4% 1W852012 0.3 – 2 0.1 < 0.6% < 0.5% < 0.4% 1

Dosys premium syringe 164 with Two-ring HandleW852014 0.1 – 1 0.05 < 0.7 1) < 0.6% < 0.4% 1 W852016 0.3 – 2 0.1 < 0.6% < 0.5% < 0.4% 1 W852018 0.5 – 5 0.5 < 0.5% < 0.4% < 0.2% 1W852020 1 – 10 1.0 < 0.5% < 0.4% < 0.2% 1

Dosys basic syringe 172 with Pistol-grip HandleW853000 0.1 – 1 0.05 < 0.7% < 0.6% < 0.4% 1W853002 0.3 – 2 0.1 < 0.6% < 0.5% < 0.4% 1W853004 0.5 – 5 0.5 < 0.5% < 0.4% < 0.2% 1W853006 1 – 10 1.0 < 0.5% < 0.4% < 0.2% 1

Dosys classic syringe 173 with Pistol-grip HandleW853008 0.025 – 0.3 0.025 < 1.8% 1) < 1.2% < 0.5% 1W853010 0.1 – 0.5 0.1 < 1.2% < 1.9% < 0.4% 1W853012 0.1 – 1 0.05 < 0.7% < 0.6% < 0.4% 1W853014 0.3 – 2 0.1 < 0.6% < 0.5% < 0.4% 1W853016 0.5 – 5 0.5 < 0.5% < 0.4% < 0.2% 1W853018 1 – 10 1.0 < 0.5% < 0.4% < 0.2% 1W853020 5 – 20 1.0 < 0.5% < 0.4% < 0.4% 1

Dosys premium syringe 174 with Pistol-grip Handle851371 0.1 – 1 0.05 < 0.7% < 0.6% < 0.4% 1851372 0.3 – 2 0.1 < 0.6% < 0.5% < 0.4% 1851373 0.5 – 5 0.05 < 0.5% < 0.4% < 0.2% 1851375 1 – 10 1.0 < 0.5% < 0.4% < 0.2% 1

Tests performed with double distilled water, using stainless steel cannula (1.2 x 50 mm) at constant working speed. 1) measured at 0.075 mL

Syringe stand

Stand holds syringe in ready-to-use position. Easy-to-clean material. Autoclavable.


W855008 Syringe Stand 1

105


Self-Refilling Syringe

Beveled, high-quality needles, stainless steel, autoclavable.

Ideal for use with 24 or 96 well microplates, the stainless steel manifolds are available in both a straight "T" style and an angular style with an offset hub. This unique dispensing system enables you to perform sample in oculations, cell feedings, and washing faster and more efficiently under sterile conditions.

Cat. No. Description Center to Center (mm) Qty/Cs

851380 4 Position, Straight, 24 well 20 1851381 8 Position, Straight, 96 well 9 1851382 4 Position, Angular, 24 well 20 1851383 8 Position, Angular, 96 well 9 6851388 12 Position, Straight, 96 well 9 1851389 12 Position, Angular, 96 well 9 1

Blunt end superior cannulas, stainless steel, autoclavable.


851411 Dispensing Cannula with Luer Lock 18 gauge x 2” for 1 mL and 2 mL Syringes 1851412 Dispensing Cannula with Luer Lock 14 gauge x 4” for 5, 10 and 20 mL Syringes 1

(medical quality silicone)


851419 Tube, syringes 0.3 - 10 mL, cut 1 m851424 Tube, syringe 20 mL, cut 1 m

Optional vial holder conversion set & reagent reservoirs for additional liquid feeding

possibilities.


W860068 Vial holder conversion set for 163 & 173 models (except 20 mL) without vial 1

W860086 Polypropylene bottle, 60 mL 1W860088 Polypropylene bottle, 125 mL 1W860090 Polypropylene bottle, 250 mL 1

Reverse modus valve set for liquid aspiration (ex. waste collection in separate container). Vented waste collector cap and enhanced tightness set recommended.


W860078 Reverse modus aspiration valve unit for 164/174 models 1W860080 Screw cap inside diameter 45 mm, with tubing connection

and filtered air outlet 1

Heavy-duty nylon brushes with tufted end and plastic handle for barrel cleaning.


W855000 For 0.3, 0.5, 1 mL barrels 6W855002 For 1, 2, 5 mL barrels 6W855004 For 5, 10, 20 mL barrels 3W855006 Set of 6 nylon brushes, 2 each 6

For safe animal mouth and nose feeding. Stainless steel, autoclavable.

Cat. No. Inside Diameter x L Qty/Pk

Drenchers, straightW855010 0.9 x 25 mm 3W855012 1.2 x 51 mm 3W855014 1.6 x 102 mm 2W855016 3.0 x 203 mm 2

Drenchers, bentW855018 0.9 x 25 mm 3W855020 1.2 x 51 mm 3W855022 1.6 x 102 mm 2W855024 3.0 x 203 mm 2

The special clear glass barrel/O-ring fitted plunger enhance tightness when demanded by application (ex. filtration, aspiration, etc.). For 5 mL syringe only.


W860072 Set includes one each plunger, O-ring, 5 mL barrel and grease 1W860074 Spare barrel for tightness set, 5 mL 6

Cat. No. ID x L (mm) Qty/Pk

W860000 0.6 x 4 12W860001 0.6 x 5 12W860002 0.6 x 8 12W860003 0.6 x 13 12W860004 0.7 x 4 12W860005 0.7 x 6 12W860006 0.7 x 7 12W860007 0.7 x 10 12W860008 0.7 x 13 12W860009 0.8 x 10 12W860010 0.8 x 20 12W860011 0.8 x 30 12W860012 0.8 x 40 12W860013 0.9 x 7 12W860014 0.9 x 10 12W860015 0.9 x 13 12W860016 1.0 x 10 12W860017 1.0 x 13 12W860018 1.0 x 15 12W860019 1.0 x 20 12W860020 1.0 x 30 12W860021 1.2 x 10 12W860022 1.2 x 15 12

Cat. No. ID x L (mm) Qty/Pk

W860023 1.2 x 20 12W860024 1.2 x 35 12W860025 1.2 x 50 12W860026 1.4 x 10 12W860027 1.4 x 20 12W860028 1.4 x 35 12W860029 1.6 x 10 12W860030 1.6 x 20 12W860031 1.6 x 25 12W860032 1.6 x 30 12W860033 1.6 x 100 12W860034 1.8 x 10 12W860035 1.8 x 20 12W860036 1.8 x 30 12W860037 2.0 x 30 12W860038 2.0 x 40 12W860039 2.0 x 50 12W860040 1.2 x 10 12W860041 1.6 x 20 12W860042 2.0 x 30 12W860043 2.0 x 40 12W860044 2.0 x 50 12

106



W851008 Glass Syringe, 0.1 – 1 mL 3W851010 Glass Syringe, 0.5 – 2 mL 3W851011 Glass Syringe, 0.2 – 5 mL 3W851012 Glass Syringe, 1 – 10 mL 3W851014 Glass Syringe, 1 – 20 mL 2W851016 Glass Syringe, 1 – 50 mL 1


W860048 Barrel, syringe, 0.3 mL 6W860049 Barrel, syringe, 0.5 mL 6851391 Barrel, syringe, 1 mL 6851392 Barrel, syringe, 2 mL 6851393 Barrel, syringe, 5 mL 6851395 Barrel, syringe, 10 mL 6W860051 Barrel, syringe, 20 mL 6


851401 Piston, 1 mL Premium Syringe 1851402 Piston, 2 mL Premium Syringe 1851403 Piston, 5 mL Premium Syringe 1851405 Piston, 10 mL Premium Syringe 1


W860052 Sleeve, 0.3, 0.5 mL barrels 6W860054 Sleeve, 1 mL barrels 6W860056 Sleeve, 2 mL barrels 6W860058 Sleeve, 5 mL barrels 6W860060 Sleeve, 10 mL barrels 6W860062 Sleeve, 20 mL barrels 3


W860064 Kit for valve, syringe 0.3 mL 1W860066 Kit for valve, syringe 0.5 mL 1W851425 Kit for valve, syringe 1 mL 1W851426 Kit for valve, syringe 2 mL 1W851427 Kit for valve, syringe 5 mL 1W851428 Kit for valve, syringe 10 mL 1W851429 Kit for valve, syringe 20 mL 1851430 Luer Lock Valve Assembly with three way valve for 1, 2, 5 & 10 mL syringes 1851431 Luer Lock Valve Assembly with three way valve for 20 mL syringe 1

Kit includes 1 spring, 2 balls, 2 washers for valve, and barrel washer.


851414 Short vent cannula, chrome plated, for 10 mL syringe 1851421 Short vent cannula, chrome plated, for 20 mL syringe 1851415 Long feed cannula, chrome plated, for 0.3 to 10 mL syringes 1851422 Long feed cannula, chrome plated, for 20 mL syringe 1

Wheaton has many products for environmental testing and for sample and liquid handling. Refer to the sections below when searching for these specific items.

BOD Bottles page 3

Bottles for Sample Collection page 7 - 28

Bottle Top Dispensers pages 99-100

Chemistry Glassware pages 67-80

Coliwasas & Other Sample Collection Devices page 129

EPA Vials & Other Vials for Sample Collection pages 142-152

Imhoff Cone for Solid Determination page 82

Pipettors with volumes up to 10 mL pages 111-116

Safety Coated Bottles pages 16 - 17

TCLP Extraction Bottles page 5

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107


Ideal for freeze dryingPre-scored to eliminate filingHeat sealable only

These ampules are designed for freeze drying, protein hydrolysis, and micro-determination of ketones and amino acids. The long stem provides a convenient means for heat sealing under vacuum. The pre-scored feature allows for easy opening. They are manufactured from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM Type I, Class A requirements.

Cat. No. Size (mL) Dia x H (mm) *OD at Top (mm) Qty/Case

651502 1 10 x 108 10 144651506 2 11.8 x 122 11.75 144651509 5 16.5 x 133 9 144651510 10 19.5 x 154 10 144

* Approximate OD

For additional information on ampules see pages 1-2.

•••

This section of the catalog includes most products used in freeze drying applications. Although these products appear in other sections of the catalog, we hope you find it convenient to have the products commonly used in the Lyophilization process located in one section of the catalog.

Lyophilization, also known as “freeze drying”, is the process of removing all liquid (water in most cases) from a sample to prolong the shelf life of

the product. Long term storage stability of biological materials has made this a preferred technique at biochemical/biotech and pharmaceutical companies and at universities and medical schools for samples such as blood plasma, vaccine, serum, tissue samples, enzymes, and microbial cultures. The food/beverage industry also uses this technique.

Stoppered Crimp SealedComponents

T he primary containers used for lyophilization tray drying are serum bottles, serum vials, and to a much lesser extent ampules. The prime accessory

is the 2 or 3 leg stopper (1) which has been designed to sit on the top of the container during the lyophilization process (2), and is inserted (3) during the stoppering step in the process. The closure (aluminum seal or threaded cap) is then applied (4). The split stopper is utilized when the dried product is sensitive to air or moisture and needs a rapid seal. Not all freeze dried materials are this sensitive or will require use with the split stopper. Wheaton can provide all the above mentioned product as well as crimpers to give your containers a secure seal.

Crimp or decap your aluminum seals with the Wheaton E-Z Crimper™ and the E-Z Decapper™. See pages 35-36 for more details.

Ideal for small volume lyophilization samplesHeat or stopper sealable

This small volume lyophilization ampule can be flame-sealed or stored with a stopper in place. They are manufactured from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM Type I, Class A requirements. Stoppers can be purchased separately.

Cat. No. Description Dia x H (mm) *OD at Top (mm) Qty/Case

651446 2 mL Vacule 12 x 86 10 144W651450 11 mm Stopper — — 144

* Approximate OD

••

Standard Ampules

Tip Seal Method: While holding the ampule body, insert the tip of the ampule stem into the sealing flame. Heat the tip approximately 3-4 mm from the top. Gently rotate the ampule while heating the tip until the melted glass fuses into a smooth dome.

Funnel-Top Ampules

Pull Seal Method: In addition to easier filling, the funnel-top ampule is easier to seal using the Pull Seal method. Hold the funnel top with one hand and the body of the ampule with the other hand. Insert the center of the ampule stem into the sealing flame. As the glass softens, rotate the ampule while pulling the funnel top off. This will require very little force and will allow the glass to seal itself. This method can be used for all ampule configurations.

Stoppered for Lyo

108


Polypropylene screw caps in 5 colorsUse with 224100-203 thin-flange stopperScrew cap size 20-400Autoclavable

Use these unlined polypropylene caps with screw neck diagnostic bottles. These solid and open top caps come in five colors and can be used with thin flange lyophilization stoppers. Screw cap thread size 20-400.


Solid Top Screw Caps240706-01 Black 300240706-02 White 300240706-03 Red 300240706-04 Blue 300240706-05 Yellow 300

Open Top Screw Caps 240716-01 Black 300240716-02 White 300240716-03 Red 300240716-04 Blue 300240716-05 Yellow 300

Use with screw caps for Screw Neck Diagnostic BottlesGray chlorobutyl/55 3-leg lyophilization stopperAutoclavable

Use this gray chlorobutyl/55 three-leg lyophilization stopper with Wheaton screw neck diagnostic bottles, in conjunction with open top caps (240716-01 series) or solid top caps (240706-01 series).

Cat. No. Stopper Size Qty/Case

224100-203 20 mm 300

Wheaton Lab 45 Media Bottles are ideal for freeze drying when used with 224100-503 or 224100-507 slotted stoppers and 240736 or 240746 screw caps. Lab 45s are manufactured from borosilicate glass and have a 45 mm screw thread finish, a writing area, and approximate volume graduations.

The wide 45 mm finish facilitates filling and retrieving contents. Sloping shoulders allow for

easy cleaning and complete drainage; the polypropylene pour ring eliminates drips. The bottles, caps, and pour rings are autoclavable.


Bottle without Cap or Pour Ring219915 Media Bottle, 100 mL, 56 x 103 mm (Dia x H) 45 12

Accessories240736 Screw Cap, White PP, without Sealing Ring 45 12240746 Screw Cap, White PP, with Center Hole 45 12240756 Pour Ring, Polypropylene 45 12240760 Pour Ring, Red ETFE 45 10224100-503 Black Lyophilization Stopper, Halobutyl/60 43 12224100-507 Black Lyophilization Stopper, EPDM 43 12

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Alternative to serum bottles and vialsCan be used for lyophilizationFits with I-Loc™ closure or screw cap with stopperSuperior chemical resistanceAutoclavable

Screw Neck diagnostic bottles are an alternative to serum bottles and vials. Each bottle has a controlled ID which allows for fit with the I-Loc™ closure or with a 20-400 screw cap with thin flange stopper. The product is made from Wheaton 400 borosilicate glass that conforms to USP Type I requirements.


Clear219360 5 21 x 48 20-400 480219361 10 25 x 61 20-400 480219362 20 32 x 66 20-400 480219364 50 42 x 80 20-400 288

Amber219370 5 21 x 48 20-400 480219371 10 25 x 61 20-400 480219372 20 32 x 66 20-400 480219374 50 42 x 80 20-400 288

For use with Screw Neck Diagnostic BottlesAdvantages of an aluminum seal with the convenience of a screw capPolypropylene screw cap with gray bromobutyl/50 stoppers Autoclavable

These closures are specifically designed for diagnostic applications and offer all the advantages of an aluminum seal and stopper with the convenience of a screw cap. The unique combination of a stopper and screw cap ensures excellent sealing characteristics making them ideal for liquid reagents. The contents can be accessed using a syringe through the stopper or with a pipette after removing the screw cap. These polypropylene closures are available in black, white, red, blue and yellow. Tolerances in the stoppers are maintained to +/-.010.



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Open Top Screw Cap

Solid Top Screw Cap

109


Fits most lyophilization applications Borosilicate glass conforms to USP

Type I requirementsShrink-wrapped partitioned modules

reduce breakage

Serum bottles are ideal for long and short term sample storage, lyophilization and vaccine/injectable drug containers. These molded bottles have excellent wall strength and chemical tolerances, which are important for storage applications. Bottles are

manufactured from Wheaton 400 borosilicate molded glass that conforms to USP Type I requirements. Bottles are packaged in shrink-wrapped partitioned modules with the exception of 2 mL size, which has no partition.


Clear Serum Bottles223712 2 7 x 13 17.5 x 35 868805 288223738 5 13 x 20 23 x 47 868805 288223739 10 13 x 20 25 x 54 868806 288223742 20 13 x 20 32 x 58 868615 288223743 30 13 x 20 37 x 63 868615 288223745 50 13 x 20 43 x 73 868615 288223746 60 13 x 20 41 x 91 868615 144223747 100 13 x 20 52 x 95 868617 144223748 125 13 x 20 54 x 107 — 144

Amber Serum Bottles223760 5 13 x 20 23 x 47 868805 288223761 10 13 x 20 25 x 54 868806 288223762 20 13 x 20 32 x 58 868615 288223763 30 13 x 20 37 x 63 868615 288223764 50 13 x 20 43 x 73 868615 288223766 100 13 x 20 52 x 95 868617 144

Note: Select aluminum seal and stopper size by using the mouth OD dimension of the bottle or vial.

Made from low extractable borosilicate glassSpecial design adds strength for freeze drying applicationsShrink-wrapped modules reduce particulate contamination

The blowback feature in the neck and specially designed bottom radius for added strength are important for lyophilization applications. The clear vials are manufac-tured from low extractable borosilicate glass that conforms to ASTM Type I, Class A and USP Type I requirements, and the amber vials are manufactured from low extractable borosilicate glass that conforms to USP Type I requirements. Vials are packaged in shrink-wrapped modules to greatly reduce particulate contamination.


Clear Tubing Vials223683 2 7 x 13 15 x 32 868804 144 223684 3 7 x 13 17 x 38 868810 144 223685 5 13 x 20 22 x 40 868805 144 223686 10 13 x 20 24 x 50 — 144 223687 20 13 x 20 30.5 x 58 — 120

Amber Tubing Vials223693 2 7 x 13 15 x 32 868804 144 223695 5 13 x 20 22 x 40 868805 144223696 10 13 x 20 24 x 50 — 144

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We offer unlined aluminum seals in a wide range of styles and sizes in natural, blue, red and green aluminum.


Open Top Unlined Aluminum Seals 224175-01 Natural 8 100 1000224175-05 Blue 8 100 1000224175-06 Red 8 100 1000224175-07 Green 8 100 1000224176-01 Natural 11 100 1000224176-05 Blue 11 100 1000224176-06 Red 11 100 1000224176-07 Green 11 100 1000224177-01 Natural 13 100 1000224177-05 Blue 13 100 1000224177-06 Red 13 100 1000224178-01 Natural 20 100 1000224178-05 Blue 20 100 1000224178-06 Red 20 100 1000224178-07 Green 20 100 1000

Center Disc Tear-Out Unlined Aluminum SealsThe center disc tears out, exposing the top of the stopper to the syringe. 224182-01 Natural 13 100 1000224182-05 Blue 13 100 1000224182-06 Red 13 100 1000224182-07 Green 13 100 1000224183-01 Natural 20 100 1000224183-05 Blue 20 100 1000224183-06 Red 20 100 1000224183-07 Green 20 100 1000

Solid Top Unlined Aluminum SealsThese seals are excellent for long-term storage of samples. 224189 Natural 11 100 1000224191 Natural 20 100 1000

Tear-Off Unlined Aluminum SealsThis seal can be completely removed and the contents readily poured from bottle. 224192-01 Natural 13 100 1000224192-05 Blue 13 100 1000224192-06 Red 13 100 1000224192-07 Green 13 100 1000224193-01 Natural 20 100 1000224193-05 Blue 20 100 1000224193-06 Red 20 100 1000224193-07 Green 20 100 1000

Open Top Unlined Seal Center Disc Tear-Out Seal

Solid Top Unlined Seal Tear Off Unlined Seal

110


Wheaton’s premium stopper selection adds confidence with purity. There are three new styles to choose from. Two of the styles feature a new rubber formulation that redefines rubber purity. The third style is a stopper that is completely coated with a fluorinated polymer coating. This coating gives the stopper a chemical resistance similar to the chemical resistance of PTFE. As a result, this coating ensures sample purity. These stoppers are a good choice if you are using harsh chemicals that require high levels of purity.

Straight Plug Stopper

Wheaton’s new Ultra Pure straight plug stoppers are made from a bromobutyl rubber compound containing the highest level of chemical purity. These stoppers are WFI compatible. They also have an ultra low level of extractables and they remain stable when exposed to gamma irradiation making them very well suited for Ready-for-Sterilization and Ready-for-Use applications.

Lyophilization Stopper

Wheaton’s new Ultra Pure lyophilization stoppers are made from a bromobutyl rubber compound containing the highest level of chemical purity. These stoppers demonstrate a very low level of moisture absorption and a low level of extractables. They are compatible with Water for Injection (WFI) applications and are not suitable for packaging applications. They are free from natural rubber or natural rubber latex. They are ideal for use in most lyophilization applications.

For Mouth Stopper Shelf Cat. No. ID x OD (mm) Style Stopper Material Pack Qty/Case

W224100-400 7 x 13 Straight Plug Gray Bromobutyl/47 100 1000W224100-401 7 x 13 2-Leg Stopper Gray Bromobutyl/46 100 1000W224100-402 7 x 13 Igloo Stopper Gray Bromobutyl/46 100 1000W224100-405 13 x 20 Straight Plug Gray Bromobutyl/46 100 1000W224100-406 13 x 20 2-Leg Stopper Gray Bromobutyl/46 100 1000W224100-407 13 x 20 3-Leg Stopper Gray Bromobutyl/46 100 1000W224100-408 13 x 20 Igloo Stopper Gray Bromobutyl/46 100 1000

Straight Plug Stopper

Wheaton’s new Complete Coat stoppers are completely coated with a polymer that has barrier properties similar to PTFE. The fluorinated polymer coating is all that comes in contact with the sample. As a result, chemical reactions due to unwanted interference with the rubber itself are virtually eliminated. These stoppers are manufactured in a silicone-free environment. A special-ized manufacturing process is used to produce extremely low levels of visible and subvisible particles. These stoppers provide users the greatest level of confidence in avoiding interference with samples.

For Mouth Stopper Shelf Cat. No. ID x OD (mm) Stopper Style Material Pack Qty/Case

W224100-420 7 x 13 Straight Plug Gray Bromobutyl/39 100 1000W224100-421 13 x 20 Straight Plug Gray Bromobutyl/39 100 1000

NewNewStraight Plug

2-Leg

3-Leg

Igloo

For Mouth Cat. No. ID x OD (mm) Stopper Material Shelf Pack Qty/Case

2-Leg Lyophilization StoppersThis all-purpose design reduces the possibility of legs sticking to each other.224100-092 7 x 13 Gray Chlorobutyl-Isoprene blend/50 100 1000224100-093 7 x 13 Gray Chlorobutyl/50 100 1000224100-192 13 x 20 Gray Chlorobutyl-Isoprene blend/50 100 1000W224100-193 13 x 20 Gray Chlorobutyl/46 100 1000224100-194 13 x 20 Gray Chlorobutyl/50 100 1000224100-282 20 x 28 Gray Chlorobutyl/50 100 1000224100-503 31 x 43 Black Halobutyl/60 — 12224100-507 31 x 43 Black EPDM — 12

3-Leg Lyophilization StoppersThe 3-leg design provides stability during lyophilization and resists cocking.224100-202 13 x 20 Gray Chlorobutyl/50 100 1000

3-Leg Thin Flange Lyophilization StopperCan be used with open top caps (240716-01 series) or solid top caps (240706-01 series).224100-203 13 x 20 Gray Chlorobutyl/55 — 300

Designed for lyophilization and freeze drying applications4, 5 and 10 mL sizeScrew threads eliminate use of crimp sealChoice of solid or open top screw cap

Heavy wall construction designed for lyophilization and freeze drying. Manufactured from Wheaton 33 low extractable clear borosilicate glass that conforms to ASTM Type I, Class A and USP Type I requirements. Stoppers and accessories listed below.

Cat. No. Size (mL) Dia x H (mm) Accepts Cap Finish Size Fits Rack Qty/Case

Vacule Vial651954 4 17 x 45 15-425 868810 200651905 5 22 x 40 22-350 868805 200651907 10 22 x 55 22-350 868805 200

Cat. No. Description Qty/Pack Qty/Case

Screw Caps and Stoppers for Cat. No. 651954, 4 mL Vacule224100-080 Natural red rubber stopper 100 1000224100-093 Gray Chlorobutyl/50 stopper, 2-Leg 100 1000240209 Cap, solid top, rubber liner, 15-425 screw thread 200 200W240509 Cap, open top, without liner, 15-425 screw thread 200 200

Screw Caps and Stoppers for Cat. No. 651905 and 651907, 5 & 10 mL Vacule224100-172 Natural red rubber stopper 100 1000224100-194 Gray Chlorobutyl/50 stopper, 2-Leg 100 1000224100-202 Gray Chlorobutyl/50 stopper, 3-Leg 100 1000239853 Cap, solid top, without liner, 22-350 screw thread 500 500

••••

2-Leg Lyophilization Stoppers

3-Leg Lyophilization Stopper

For a broader selection of aluminum seals and rubber stoppers see the Caps and Closures Section, pages 32-34.

111


Socorex

Swift-set Instant calibrationThe swift-set user calibration system with integrated key and locking mechanism makes tedious procedures a thing of the past. Easy and precise, it is in full compliance with international standards and recommendations.

Smart and reliable volume adjustmentState-of-the-art micrometric volume setting is performed smoothly and precisely by turning push button. Finely machined click-stops and free rotating cap prevent any unwanted volume alteration.

JusTip™ Tip ejection – easier than everErgonomically positioned, the large surface, soft padded ejector button provides for low-pressure activation. In addition to ease of tip ejection, the shaft height adjustment system called JusTip™ – controlled by efficient click-stops – allows a wide selection of tips to tightly fit the nozzle.

Optimal working positionIn addition to ergonomic design and perfect hand fitting of the pipette, the multichannel volumetric module revolves for added user comfort.

Adjustable tip ejectorPosition of tip ejector can be adjusted in no time within a 4 mm span to perfectly match to tip used. Bowed shape of ejector head guarantees effortless, easy sequential tip ejection.

manual

Digital display window for permanent volume visibilityExceptional, user-friendly ergonomics Shock, heat, chemicals and UV light resistanceJusTip™ adjustable tip ejection mechanismSwift-set user calibration systemLightweight, 3 - 7 oz (88 - 203 g) depending on modelThree year warranty

The Acura® manual are precision pipettes designed and built with the users in mind. They combine high-tech materials with exceptional, user-friendly ergonomics. Whether in research or routine applications, they bring much more than superior performance and make pipetting safe and agreeable. Entirely autoclavable at 121°C/250°F.

The Acura® manual fits everybody’s hand with or without a glove. Ergonomic shape, light weight and ultra-soft plunger travel offers a pleasant pipetting feeling. The Acura manual models include fixed and adjustable volume pipettes.

815 fixed volumes (1 – 1000 μL) See page 113835F fixed volumes (2 – 10 mL) See page 113825 micro variable volumes (0.1 – 1000 μL) 835 macro variable volumes (0.2 – 10 mL) 855 multi 8 and 12 channel (0.5 – 350 μL)

•••••••

•••••

Maximum comfort from ergonomic shape, lightweight feel, & ultra-soft plunger activation

Reliable micrometric volume adjustment keeps

gloves from tearing

Ergonomically positioned tip ejection allows for easy tip disposal

Swift-set calibration system fully complies with international standards & recommendations

Digital display is visible at all times

during pipetting

112Socorex


Inaccuracy (E%) Imprecision (CV%) Qty/Cat. No. Volume Division Tip Style Min. Vol Mid. Vol. Max. Vol. Min. Vol Mid. Vol. Max. Vol. Case

Acura® manual 825 micro W810300 0.1 – 2 μL 0.002 μL Ultra 10 μL < ± 6.0% 1) < ± 4.0% < ± 2.0% < 5.0% 1) < 3.3% < 1.5% 1W810302 0.5 – 10 μL 0.01 μL Ultra 10 μL < ± 2.5% 2) < ± 1.8% < ± 1.0% < 1.8% 2) < 1.2% < 0.5% 1W810304 1 – 10 μL 0.01 μL 200 μL < ± 2.5% < ± 1.8% < ± 1.0% < 2.5% < 1.6% < 0.7% 1W810306 2 – 20 μL 0.02 μL 200 μL < ± 2.5% < ± 1.8% < ± 1.0% < 1.7% < 1.1% < 0.5% 1W810308 5 – 50 μL 0.1 μL 200 μL < ± 1.5% < ± 1.3% < ± 1.0% < 1.0% < 0.7% < 0.4% 1W810310 10 – 100 μL 0.1 μL 200 μL < ± 1.5% < ± 1.2% < ± 0.8% < 1.0% < 0.6% < 0.2% 1W810312 20 – 200 μL 0.2 μL 200 μL < ± 1.5% < ± 1.1% < ± 0.6% < 0.6% < 0.4% < 0.2% 1W810314 100 – 1000 μL 1 μL 1000 μL < ± 1.5% < ± 1.0% < ± 0.5% < 0.5% < 0.4% < 0.2% 1

Acura® manual 835 macro W810316 0.2 – 5 mL 0.01 mL 5 mL < ± 1.5% < ± 1.1% < ± 0.6% < 0.6% < 0.5% < 0.4% 1W810318 1 – 10 mL 0.01 mL 10 mL < ± 1.5% < ± 0.7% < ± 0.5% < 0.5% < 0.3% < 0.2% 1

Inaccuracy (E%) Imprecision (CV%) Qty/Cat. No. Volume Division Tip Style Min. Vol Mid. Vol. Max. Vol. Min. Vol Mid. Vol. Max. Vol. Case

Acura® manual 855 8-channel W810600 0.5 – 10 μL 0.01 μL Ultra 10 μL < ± 3.5% 1) < ± 2.5% < ± 1.5% < 3.0% 1) < 2.0% < 1.0% 1W810602 5 – 50 μL 0.1 μL 200 μL < ± 1.0% < ± 0.9% < ± 0.8% < 1.0% < 0.7% < 0.4% 1W810603 20 – 200 μL 0.2 μL 200 μL < ± 0.9% < ± 0.8% < ± 0.7% < 0.6% < 0.5% < 0.3% 1W810604 40 – 350 μL 0.4 μL 350 μL < ± 1.0% < ± 0.9% < ± 0.8% < 0.6% < 0.5% < 0.3% 1

Acura® manual 855 12-channelW810606 0.5 – 10 μL 0.01 μL Ultra 10 μL < ± 3.5% 1) < ± 2.5% < ± 1.5% < 3.0% 1) < 2.0% < 1.0% 1W810608 5 – 50 μL 0.1 μL 200 μL < ± 1.0% < ± 0.9% < ± 0.8% < 1.0% < 0.7% < 0.4% 1W810609 20 – 200 μL 0.2 μL 200 μL < ± 0.9% < ± 0.8% < ± 0.7% < 0.6% < 0.5% < 0.3% 1W810610 40 – 350 μL 0.4 μL 350 μL < ± 1.0% < ± 0.9% < ± 0.8% < 0.6% < 0.5% < 0.3% 1

Performance values obtained with double distilled water at constant temperature (± 0.5°C) comprised between 20 and 25°C in accordance with EN ISO 8655. 1) measured at 0.5 μL 2) measured at 1 μL

See the cost-effective Triopack on page 113.

Pipette Tips. Choose from a wide selection of tips made from high grade, virgin metal-free polypropylene.

Nozzle Filters add protection from pipette contamination on Socorex Acura manual 810 dilution pipette, 835 (2, 5 and 10 mL), Calibra digital 832 (2 and 10 mL) and Acura electro 935 (5 and 10 mL) model pipettes.

Pasteur Pipette Adapter allows for use of glass Pasteur pipets with Acura manual 835 5 mL model. Adapter is provided with Calibra digital 832 2 mL model.

Smartie Caps provide a way to color code your Socorex pipette with 14 different colors.

Reagent Reservoirs are designed for maximum recovery of liquid reagents.

Work Stations provide a space saving storage place for all your pipettes, including other brand pipettes.

How to Use a Socorex Pipette. Increase the longevity of your Socorex pipettes by helping students and new laboratory personnel learn the proper way

to use and store pipettes. See page 162 for details.

Nozzle Filters Pasteur Pipette Adapter

Model 337 Model 340

When needing calibration and repair for your Socorex pipettes contact Wheaton Customer Service.

For pipette tips and accessories see pages 119-120.

113


Socorex

manualThree adjustable micropipettesLarge choice of volumesCost effective prices

This ready-to-use set includes three Acura® manual adjustable pipettes, tip samples, QC certificate and operating instructions. There are eight different Triopacks available, each covering specific volumes. The full coverage stretches between 0.1 μL and 10 mL. Triopack brings a major price/quality advantage matching budget constraints.

•••

fixed

1 μL to 10 mLSlim ergonomic design and light weightUltra-soft plunger activation JusTip™ adjustable tip ejection systemUser calibration with integrated keyColor coding

••••••

Performance values obtained with double distilled water at constant temperature (± 0.5°C) comprised between 20 and 25°C in accordance with EN ISO 8655.

Inaccuracy Inprecision Cat. No. Volume Tip style (E%) (CV%) Qty/Case

Acura fixed 815 W810000 1 μL Ultra 10 μL < ± 2.0% < 1.0% 1W810002 5 μL Ultra 10 μL < ± 1.4% < 1.0% 1W810004 10 μL Ultra 10 μL < ± 0.7% < 0.7% 1851206 10Y μL 200 μL < ± 0.7% < 0.8% 1W810006 15 μL 200 μL < ± 0.7% < 0.5% 1W810008 20 μL 200 μL < ± 0.75% < 0.5% 1W810010 25 μL 200 μL < ± 0.7% < 0.6% 1W810012 30 μL 200 μL < ± 0.7% < 0.6% 1W810014 40 μL 200 μL < ± 0.7% < 0.5% 1W810016 50 μL 200 μL < ± 0.7% < 0.4% 1W810018 60 μL 200 μL < ± 0.7% < 0.5% 1W810020 70 μL 200 μL < ± 0.7% < 0.4% 1W810022 75 μL 200 μL < ± 0.7% < 0.4% 1W810024 80 μL 200 μL < ± 0.7% < 0.35% 1W810026 90 μL 200 μL < ± 0.7% < 0.3% 1W810028 100 μL 200 μL < ± 0.7% < 0.3% 1W810030 120 μL 200 μL < ± 0.7% < 0.4% 1W810032 150 μL 200 μL < ± 0.7% < 0.3% 1W810034 200 μL 200 μL < ± 0.7% < 0.3% 1W810036 250 μL 1000 μL < ± 0.7% < 0.4% 1W810038 300 μL 1000 μL < ± 0.7% < 0.4% 1W810040 400 μL 1000 μL < ± 0.7% < 0.3% 1W810042 500 μL 1000 μL < ± 0.7% < 0.3% 1W810044 600 μL 1000 μL < ± 0.7% < 0.3% 1W810046 700 μL 1000 μL < ± 0.7% < 0.2% 1W810048 750 μL 1000 μL < ± 0.7% < 0.2% 1W810050 800 μL 1000 μL < ± 0.7% < 0.2% 1W810052 900 μL 1000 μL < ± 0.7% < 0.2% 1W810054 1000 μL 1000 μL < ± 0.6% < 0.2% 1

Acura fixed 835F W810056 2 mL 2 mL < ± 0.8% < 0.5% 1W810058 2.5 mL 5 mL < ± 0.8% < 0.5% 1W810060 5 mL 5 mL < ± 0.7% < 0.4% 1W810062 10 mL 10 mL < ± 0.5% < 0.2% 1

Cat. No. Contents (Micro/macropipette models)

W810319 2 μL 10 μL 50 μL (0.1 – 2) (0.5 – 10) (5 – 50)W810320 2 μL 20 μL 200 μL (0.1 – 2) (2 – 20) (20 – 200)W810321 10Y μL 50 μL 200 μL (1 – 10) (5 – 50) (20 – 200)


W810323 10Y μL 100 μL 1000 μL (1 – 10) (10 – 100) (100 – 1000)W810324 20 μL 200 μL 1000 μL (2 – 20) (20 – 200) (100 – 1000)W810325 50 μL 200 μL 1000 μL (5 – 50) (20 – 200) (100 – 1000)


W810326 100 μL 1000 μL 5 mL (10 – 100) (100 – 1000) (0.5– 5)W810327 1000 μL 5 mL 10 mL (100 – 1000) (0.5 – 5) (1 – 10)

Smartie Caps provide a way to color code your Socorex pipette with 14 different colors. See page 120 for details.

114Socorex


Two pre-calibrated fixed volumes – no setting requiredSmooth activation, excellent ergonomics JusTip™ system for height adjustment of tip ejectorEasy maintenance, cleaning and disinfectionFully autoclavable 121°C/250°FThree year warranty

••••••

No setting requiredTwo fixed volumes within one plunger travel.

Innovative dual calibrationBoth volumes of 1 mL and 0.1 mL can be calibrated independently from each other.

JusTip™ ejectorTurn left or right to adjust ejector to strawtip position on the nozzle.

Straw tipsFirst class polypropylene (L: 190 mm, Inside Dia: 4 mm) specially designed for narrow vials and Stomacher® bags. No blocking by particles in suspension.

1 2 3

Air displacement pipette with two pre-calibrated steps allows subsequent pipetting of 1 and 0.1 mL of the same liquid. Metal nozzle fits long straw tip to aspirate from narrow or deep reservoirs (i.e. Stomacher® bags). Ideal alternative to the graduated glass pipettes when performing serial 1:10 dilutions in bacteriology.

Simple operation

Depress plunger button in full, then release slowly to aspirate 1.1 mL

Depress plunger button to first stop, thus dispensing 1 mL in Petri dish

Depress plunger button to second stop, thus dispensing residual 0.1 mL in next Petri dish

1

2

3

Inaccuracy Inprecision Cat. No. Description Volume (E%) (CV%) Qty/Case

851343 Acura manual 810 1 mL < ± 0.4% < ± 0.4% 0.1 mL < ± 2.0% < ± 2.5% 1

Accessories851344 Straw Tips, Sterile, Polypropylene 190 mm in length — — — 20 x 25/pk







How to Use a Socorex Pipette. Increase the longevity of your Socorex pipettes by helping students and new laboratory

personnel learn the proper way to use and store pipettes. See page 162 for details.


Model 337 Model 340


115


Socorex

Natural ergonomics

The Acura® electro offers shape, balance and working ergonomics resembling those of a manual instrument. Comfort is so greatly improved that pipetting and resulting consistency are outstanding!

Unique “right or left” display reading

User converts displayed information for right or left side reading. All necessary elements are visible at a glance during programming, pipetting and calibration steps.

Variable working speed

Located on the front side, the speed selector allows instant change even during the pipetting process. In addition, slow speed can be activated at any time by gentle touch of the start button.

Time efficient maintenance

Clever pipette construction limits maintenance to a minimum and requires no tool for disas-sembling the volumetric modules. Whenever sterilization is needed, the volumetric modules can be autoclaved at 121°C/250°F.

Tip ejection – easier than ever

Maximum efficiency is obtained with a large, ergonomically located ejector button. The shaft height adjustment system called JusTip™ (4 mm span) – controlled by efficient click-stops – allows a wide selection of tips for a tight nozzle fit.

On multichannel models, bowed shape of ejector head adds to effortless, easy sequential tip ejection.

Optional battery charging standSpace-saving stand holds up to three battery packs simultaneously. The ideal charging and storage accessory for spare batteries, which are then available at any time.

electro

Smart battery packs

The NiMH battery pack is located in the power handle. It can be removed and changed in a second. New 4.8V tension enables fast battery charging (<1.5 hours) and provides extended working autonomy (>3000 consecutive pipetting cycles). When unused, the Acura® electro automatically goes on energy saving stand-by.

Calibration

Control software provides for instant access to calibration menu. Adjustments are performed directly on the instrument’s keyboard. Any inaccurate plunger movement is detected by the microproces-sor which warns the user with an error message.

Pipette charging standStand allows charging and storing of up to three electronic pipettes or battery packs simultaneously. Each position is fitted with a red/green charging LED. It is supplied with every Acura® electro basic package.

Optimized ergonomics and working comfortLightweight Easy, intuitive, self-teaching programRight or left display readingSwift-interchange battery packInnovative, adjustable tip ejectionElectronic control unit fits other volumetric modulesOne year warranty

The Acura® electro is making electronic pipetting simpler and safer than ever. The instrument includes a micro-processor-controlled motor and a rechargeable 4.8 V battery. Intuitive control software allows for quick access to five working modes and many volumes to select from.

Model 925 (0.5 – 1000 uL) Model 935 (0.25 – 10 mL) Model 955 8 and 12 channel (0.5 – 350 uL)

••••••••

•••

Energy Star® qualified power supply for a better environment

116Socorex


To increase pipetting flexibility, one electronic control unit accommodates the other volumetric modules available separately. This alone contributes to make electronic pipetting affordable to all budgets. Initial calibration is necessary before very first use of each additional or replacement volumetric module. Instrument memory retains the calibration data.

Forward pipetting

Reverse pipetting

Step-wise pipetting Dilution (up to 3 liquids)

Mixing

Includes electronic pipette with individual QC certificate, pipette charging stand, fast charger with AC adapter and cord, one additional 4.8 V battery pack, Qualitips® pipette tips samples and operating instructions.

Cat. No. Cat. No. Cat. No. Inaccuracy (E%) Imprecision (CV%) Qty/Basic Pkg Pipette Volumetric Module Volume Division Tip Style Min. Vol Mid. Vol. Max. Vol. Min. Vol Mid. Vol. Max. Vol. Case

Acura® electro 925 micro W810908-A W810900 W820015 0.5 – 10 μL 0.05 μL Ultra 10 μL < ± 2.5%1) < ± 1.8% < ± 1.0% < 1.8%1) < 1.2% < 0.5% 1

W810910-A W810902 W820016 2.5 – 50 μL 0.25 μL 200 μL < ± 2.5%2) < ± 1.6% < ± 0.7% < 1.5%2) < 0.9% < 0.3% 1

W810912-A W810904 W820017 10 – 200 μL 1.0 μL 200 μL < ± 1.5%3) < ± 1.1% < ± 0.6% < 0.7%3) < 0.5% < 0.2% 1

W810914-A W810906 W820018 50 – 1000 μL 5.0 μL 1000 μL < ± 1.5%4) < ± 1.0% < ± 0.5% < 0.5%4) < 0.4% < 0.2% 1

Acura® electro 935 macroW810916-A W810916 W820024 0.25 – 5 mL 0.05 mL 5 mL < ± 1.0%5) < ± 0.9% < ± 0.7% < 0.6%5) < 0.5% < 0.3% 1

W810918-A W810918 W820025 0.5 – 10 mL 0.05 mL 10 mL < ± 0.8%6) < ± 0.7% < ± 0.5% < 0.4%6) < 0.3% < 0.2% 1

Acura® electro 955 8-channelW810924-A W810924 W820026 0.5 – 10 μL 0.05 μL Ultra 10 μL < ± 3.5%1) < ± 2.5% < ± 1.5% < 3.0% 1) < 2.0% < 1.0% 1

W810926-A W810926 W820027 2.5 – 50 μL 0.25 μL 200 μL < ± 1.0%2) < ± 0.9% < ± 0.8% < 1.0% 2) < 0.7% < 0.4% 1

W810928-A W810928 W820028 20 – 350 μL 5.0 μL 350 μL < ± 1.0%7) < ± 0.9% < ± 0.8% < 0.6% 7) < 0.4% < 0.3% 1

Acura® electro 955 12-channelW810930-A W810930 W820029 0.5 – 10 μL 0.05 μL Ultra 10 μL < ± 3.5%1) < ± 2.5% < ± 1.5% < 3.0%1) < 2.0% < 1.0% 1

W810932-A W810932 W820030 2.5 – 50 μL 0.25 μL 200 μL < ± 1.0%2) < ± 0.9% < ± 0.8% < 1.0%2) < 0.7% < 0.4% 1

W810934-A W810934 W820031 20 – 350 μL 5.0 μL 350 μL < ± 1.0%7) < ± 0.9% < ± 0.8% < 0.6%7) < 0.4% < 0.3% 1

Performance values obtained with double distilled water at constant temperature (± 0.5°C) comprised between 20 and 25°C in accordance with EN ISO 8655. Performance measured at 1) 1 μL 2) 5 μL 3) 20μL 4) 100 μL 5) 0.5 mL 6) 1 mL 7) 40 μL


W820019 Pipette charging stand, 3 charging positions, holds micropipettes or power handles 1W820020 Compact charging stand, 3 charging positions, holds power handles only 1W820021 Spare battery pack (power handle) 1W810929-A Spare battery pack (power handle) duo pack 2W820022-A Power supply with AC adapter, 100-240 V and cord 1










Model 337 Model 340

Powerful intelligenceThe “no gimmick” intuitive control software does not require any particular skills. Avoiding compli-cated programming steps, there are only a few but-tons on the keyboard to give access to the various modes within seconds.

Fast charger with AC adapter


117


Socorex

Comfort and safetyEasy and glove friendly volume setting and tip ejection bring added comfort. No unwanted volume alteration or detipping while working.

Key volume adjustmentExample: from 10 to 50 μL in half a revolution of the setting wheel placed in its normal position.

Fine-tuning adjustmentExample: from 50 to 55.5 μL in half a revolution of the setting wheel in pulled position.

Color codingAttractive colored plates and ejector buttons for easy identification of volume range and tip style.

Easy in-lab calibrationThe calibration screw is located under the rubber pad. Engraved divisions help calibrate instrument quickly and precisely. Calibration key supplied with the instrument.

Pasteur Pipette AdapterPVDF adapter fits pipette nozzle to accommodate standard glass Pasteur pipettes. FPM O-rings care for effective tightness. Included with each 2 mL Calibra® model.

Wind-free instant volume adjustmentUnsurpassed performance and calibration stability Robust, long-lasting constructionShock, heat, chemicals and UV light resistanceReliable user calibrationAutoclavable at 121°C/250°F fully assembledThree year warranty

Model 822 micro (0.2 – 1000 μL) Model 832 macro (0.2 – 10 mL) Model 852 8 (1 – 200 μL) and 12 channel (10 – 200 μL)

A highly reliable pipette line with instant volume adjustment. The combination of mechanical precision, comfort and ease of use in the same instrument makes the Calibra® digital one of the smartest high-end micropipettes in the market.

•••••••

•••

Instant volume settingMajor feature of the Calibra® line, the twin cam system is fitted with pre-calibrated steps providing for true digital volume entry and numerical display. The dual incrementation allows fast volume setting without tedious winding.

118Socorex


360° rotationIn addition to ergonomic hand fitting, the pipette volumetric module (lower assembly) revolves for added user comfort.

Sequential tip ejectionSpecial shape of ejector head guarantees effortless, sequential easy tip ejection.

Inaccuracy (E%) Imprecision (CV%) Qty/Cat. No. Volume Division Tip Style Min. Vol Mid. Vol. Max. Vol. Min. Vol Mid. Vol. Max. Vol Case

Calibra® digital 822 micro851160 0.2 – 2 μL 0.1 μL Ultra 10 μL < ± 6.0%1) < ± 4.0% < ± 2.0% < 5.0%1) < 3.3% < 1.5% 1851162 1 – 10 μL 0.05 μL Ultra 10 μL < ± 2.0% < ± 1.5% < ± 1.0% < 2.0% < 1.3% < 0.5% 1851163 2 – 20 μL 0.1 μL 200 μL < ± 2.0% < ± 1.5% < ± 1.0% < 1.7% < 1.1% < 0.5% 1851164 10 – 100 μL 0.5 μL 200 μL < ± 1.0% < ± 0.9% < ± 0.8% < 1.0% < 0.6% < 0.2% 1851166 20 – 200 μL 1.0 μL 200 μL < ± 1.0% < ± 0.9% < ± 0.8% < 0.6% < 0.4% < 0.2% 1851168 100 – 1000 μL 5.0 μL 1000 μL < ± 0.7% < ± 0.6% < ± 0.5% < 0.5% < 0.4% < 0.2% 1

Calibra® digital 832 macro851340 0.2 – 2 mL 0.01 mL 2 mL < ± 1.5% < ± 1.0% < ± 0.5% < 0.5% < 0.3% < 0.2% 1851345 1 – 10 mL 0.1 mL 10 mL < ± 1.5% < ± 1.0% < ± 0.5% < 0.3% < 0.3% < 0.15% 1

Calibra® digital 852 8-channelW851180-2 1 – 10 μL 0.05 μL Ultra 10 μL < ± 3.5% < ± 2.5% < ± 1.5% < 3.0% < 2.0% < 1.0% 1851183-2 10 – 100 μL 0.5 μL 200 μL < ± 1.0% < ± 0.9% < ± 0.7% < 1.0% < 0.7% < 0.4% 1851187-2 20 – 200 μL 1.0 μL 200 μL < ± 0.9% < ± 0.9% < ± 0.7% < 0.6% < 0.4% < 0.3% 1

Calibra® digital 852 12-channel851184-2 10 – 100 μL 0.5 μL 200 μL < ± 1.0% < ± 0.9% < ± 0.7% < 1.0% < 0.7% < 0.4% 1851188-2 20 – 200 μL 1.0 μL 200 μL < ± 0.9% < ± 0.9% < ± 0.7% < 0.6% < 0.4% < 0.3% 1

Performance values obtained with double distilled water at constant temperature (± 0.5°C) comprised between 20 and 25°C in accordance /with EN ISO 8655. 1) measured at 0.5 μL

Alternative ejector headImproved compatibility with long neck tips.









For pipettes tips and accessories see pages 119-120.


Model 337 Model 340

When needing calibration and repair for your Socorex pipettes contact Wheaton Customer Service.

119


Socorex

Made from high grade, virgin, metal-free polypropylene

Wide selection, superior qualityPrecision injection molding care for air-tight fitLot-by-lot consistency

•

•••

manual electro

Bulk or rack availabilityHigh-density filter prevents aerosol contaminationDNase and RNase-free filter tipsPyrogen-free sterile filter tips

••••

digital 815 825/925 835/935 855/955 822 832 852

DNas

e, R

Nase

Fre

e

Filte

red

Pyro

gen

Free

Ster

ile

up to

10

μL

10Y/

15/2

0 μL

25 to

100

μL

120

to 2

00 μ

L

250

to 1

000

μL

0.1

to 2

μL

0.5

to 1

0 μL

1 to

10

μL (Y

)

2 to

20

μL

5 to

50

μL

10 to

100

μL

20 to

200

μL

100

to 1

000

μL

2 m

L, 2

.5 m

L

0.5

to 5

mL

1 to

10

mL

0.5

to 1

0 μL

5 to

50

μL

20 to

200

μL

40 to

350

μL

0.2

to 2

μL

1 to

10

μL

2 to

20

μL

10 to

100

μL

20 to

200

μL

100

to 1

000

μL

0.2

to 2

mL

1 to

10

mL

1 to

10

μL

10 to

100

μL

20 to

200

μL

Cat No. Description Qty/Case

851172 Short Tip, 10 racks of 100 1000 N N N N X X X X X X X

851174 Long Tip, 4 racks of 200 800 N N N N X X X X X X X

851176 0.2 mm gel seq, 4 racks of 200 800 N N N N X X X X X X X

851178 0.4 mm gel seq, 4 racks of 200 800 N N N N X X X X X X X

W851239-01 10 racks of 96 tips 960 N N N N X X X X X X X

W851239-02 10 racks of 96 tips 960 N N N Y X X X X X X X

W851239-03 Bulk pack of 1000 tips 1000 N N N N X X X X X X X


851179-01 Individually wrapped 1000 Y Y Y Y X X X X X X X X X X

851179-02 10 racks of 96 tips 960 Y Y Y Y X X X X X X X X X X


851246 10 racks of 96 tips 960 N N N N X X X X X X X X X X X X X X X

851247 10 racks of 96 tips 960 N N N Y X X X X X X X X X X X X X X X

851248 Bulk pack of 1000 tips 1000 N N N N X X X X X X X X X X X X X X X

851271 Bulk pack of 1000 tips 1000 N N N N X X X X X X X X X X X X X X X

851272 10 racks of 96 tips 960 N N N N X X X X X X X X X X X X X X X


851181-01 Bulk pack of 1000 tips 1000 Y Y N N X X X X X X X X X X X1) X X X X X

851181-02 10 racks of 96 tips 960 Y Y N N X X X X X X X X X X X1) X X X X X

851181-03 Individually wrapped 1000 Y Y Y Y X X X X X X X X X X X1) X X X X X

851181-04 10 racks of 96 tips 960 Y Y Y Y X X X X X X X X X X X1) X X X X X


851276 Bulk pack of 1000 tips 1000 N N N N X X X

851277 10 racks of 100 tips 1000 N N N N X X X

851180-01 Individually wrapped 1000 N Y N Y X X X

851180-02 10 racks of 96 tips 960 N Y N N X X X


851355 2 mL - bulk pack of 250 tips 250 N N N N X X

851357 5 mL - bulk pack of 250 tips 250 N N N N X

851358 10 mL - bulk pack of 100 tips 100 N N N N X X

W851362 5 mL - 2 bulk packs of 250 tips 500 N N N N X

W851363 5 mL - 2 racks of 50 tips 100 N N N N X

1) Tip capacity is 300 μL

120Socorex


Nozzle Filters for PipettesAdd protection against liquid fill or pipette contamination. Filters are inter-changeable, non sterile, non autoclavable. Fits Socorex macro model pipettes.

Cat. No. Fits Material Qty/Bag

851341 Calibra® manual 832, 2 mL Cellulose 250W851342 Acura® manual 835, 2 and 5 mL Cellulose 250 Acura® electro 935, 5 mL 851346 Calibra® digital 832, 10 mL PP fibers 100 Acura® electro 935, 10 mL Acura® manual 835, 10 mL

Pasteur Pipette AdapterPVDF adapter fits pipette nozzle to accommodate standard glass Pasteur pipettes. FPM O-rings care for effective tightness. Included with each 2 mL Calibra® model. Optional for Acura® manual 835 and Acura® electro 935 5 mL models.

Cat. No. Description Qty/Pack

W820023 For model 835 and 935, 5 mL 1

Disposable Pasteur PipettesCat. No. Overall Length (mm) Tip Length (mm) Body Length (mm) Shelf Pack Qty/Case

357331 146 45 101 200 1000357335 228 108 120 200 1000

Smartie CapsThe Acura® pipettes have color-coded display windows and smarties button caps. Other color combinations are desirable for individual, lab or department identification; choose among 14 colors of smarties, or pick the smartie mix which contains all colors.

Cat. No. Color Qty/Pack Cat. No. Color Qty/Pack

Smarties plunger caps W820000 White 6 W820007 Rose 6W820001 Grey 6 W820008 Pink 6W820002 Vanilla 6 W820009 Purple 6W820003 Lemon 6 W820010 Ice blue 6W820004 Yellow 6 W820011 Blue 6W820005 Orange 6 W820012 Mint 6W820006 Red 6 W820013 Green 6

Smartie mix W820014 Assorted colors 14

Reagent ReservoirsCat. No. Style Volume (mL) Sterile Size (mm) Qty/Pack

W851364 PP, V-shaped, no lid 70 No 58 x 132 x 25 20W851365 PP, V-shaped, hinged lid 125 No 83 x 121 x 34 20W851239 PS, 8-channel, no lid 40 Yes 83 x 126 x 13 10W851368 PS, 12-channel, no lid 40 Yes 83 x 126 x 13 10851249 PP, V-shaped, no lid 75 No 48 x 127 x 32 10851250 PP, V-shaped, no lid 75 Yes 48 x 127 x 32 10

Materials are PP (Polypropylene) and PS (Polystyrene).

7-position work stationSpace-saving pipette stand holds up to seven single-channel pipettes of most brands. Made of sturdy polyamid material, easy to clean. Anti-slip pads guarantee bench stability. The work station fits new and old generations of Socorex and other brand pipettes.

3-position multi- and single- channel pipette work stationInnovative design fits all Calibra®and Acura® models. Easy reach of each instrument. Heavy bottom plate and anti-slip pads guarantee high stability. Made of polyamid material, easy to clean.

Work station 340 also fits the Acura® 810 dilution pipette and Acura® 865 microdispenser pipettes.

Cat. No. Color code Fits Qty/Pack

Universal work station model 337 W851361 Light grey 7 single-channel pipette 1W851354 Ice blue 7 single-channel pipette 1W851356 Vanilla yellow 7 single-channel pipette 1W851359 Mint green 7 single-channel pipette 1W851360 Pastel rose 7 single-channel pipette 1

Work station model 340 W851369 Light grey 3 multi- or single-channel pipette 1

Work Station 337 Work Station 340

Pasteur Pipette Adapter Disposable Pasteur Pipette

121


Socorex

Color codingPipette, plunger tip and glass capillary bear color coding for easy volume identification.

Work station

For two positive displacement pipettes

Cat. No. Color Qty/Pk

W830030 Red 1

4-finger activationSetting knobs bear clear indication

of volume and number of aliquots Three positive displacement syringes,

color coded Self-locking mechanismOne year warranty

••

•

••

Clear setting indicationAfter inserting disposable syringe, select ap-propriate volume. Markings on interchangeable selector buttons allow direct reading of set volume and corresponding number of aliquots.

Color codingEcostep™ syringe and elector button bear color coding to eliminate any setting error.

7349362924201815141211109887776

101520253035404550556065707580859095100

5075100125150175200225250275300325350375400425450475500

50075010001250150017502000225025002750300032503500375040004250450047505000

Stand sold separately

Syringe Sterile Performance Cat. No. Volume Color Capacity Ind. Wrapped at Inaccuracy (E%) Imprecision Qty/Case

Ecostep Syringes851604 10 – 100 μL Yellow 0.75 mL no 20 μL < ± 2.0% < 2.3% 100/box851616 10 – 100 μL Yellow 0.75 mL yes 100 μL < ± 1.0% < 0.6% 100/box851608 50 – 500 μL Blue 3.75 mL no 100 μL < ± 1.0% < 1.8% 100/box851620 50 – 500 μL Blue 3.75 mL yes 500 μL < ± 0.7% < 0.4% 100/box851612 500 – 5000 μL Red 37.5 mL no 1000 μL < ± 0.5% < 1.2% 100/box851624 500 – 5000 μL Red 37.5 mL yes 5000 μL < ± 0.5% < 0.4% 100/box

Performance values obtained with double distilled water at constant temperature (± 0.5°C) comprised between 20 and 25°C.

Highly comfortable pipette intended for reliable repeat dispensing within 10 to 5000 μL. Unique trigger action mechanism eliminates thumb fatigue. Selected materials offer good shock resistance. Compact shape greatly limits internal instrument contamination. Large selection among 53 different volumes; up to 73 doses per filling.


851601 Stepper™ 411 incl. 3 color coded selector buttons, adapter, Ecostep™ syringe samples 1851625 Replacement adapter for red Ecostep™ syringe 5W835010 Stand for one Stepper 1

Step-wise volume adjustmentInterchangeable plunger and glass capillary Matching color codes on pipette, plunger and capillary Stainless steel, PTFE-tipped plunger Three year warranty

Pipettes with PTFE-tipped plunger provide for positive displacement of sample in a precision capillary tube. Optimal accuracy and reproducibility when pipetting high-density, viscous, foaming or volatile liquids. Five models cover a range from 1 to 200 μL.

•••••

Inaccuracy (E%) Imprecision (CV%) Cat. No. Volume Color Code Min. Vol Mid. Vol. Max. Vol. Min. Vol Mid. Vol. Max. Vol. Qty/Case

851295 1-2-3-4-5 μL White < ± 3.0% < ± 2.3% < ± 2.0% < 2.0% < 1.4% < 0.8% 1851299 5-10-15-20-25 μL 2x white < ± 1.4% < ± 1.0% < ± 0.8% < 0.8% < 0.6% < 0.4% 1851303 10-20-30-40-50 μL Green < ± 0.7% < ± 0.7% < ± 0.7% < 0.7% < 0.5% < 0.3% 1851307 60-75-80-90-100 μL Blue < ± 0.7% < ± 0.7% < ± 0.7% < 0.3% < 0.3% < 0.3% 1851311 100-120-150-175-200 μL Red < ± 0.7% < ± 0.7% < ± 0.7% < 0.3% < 0.3% < 0.3% 1

Performance values obtained with double distilled water at constant temperature (± 0.5°C) comprised between 20 and 25°C /in accordance with EN ISO 8655.

Cat. No. Color code Qty/Pack

Spare Glass Capillaries851321 White 200851322 2x white 200851323 Green 200851324 Blue 200851325 Red 100

Cat. No. Color code Qty/Pack

Spare Plungers851330 Steel 5851331 White 5851332 Green 5851333 Blue 5851334 Red 5

Fingertip

ActivationFingertip

Activation

122Socorex


Excellent ergonomics Instrument shape and lightweight offer convenient pipetting comfort. Smooth plunger activation drastically reduces hand fatigue.

Convenient dispensingReliable valve system ensures high performance dispensing, dose after dose. It revolves to allow selection of the best working position.

Swift-set calibration Easy and precise system with integrated key and locking mechanism, making tedious procedures a thing of the past.

Choice of feeding sourcesSupplied with a 90 cm silicone tubing, the instrument also fits optional feeding sources:

bottle

vial

syringe

Great distribution flexibilityOutlet nozzle fits any Luer Lock needles and cannulas. Optional PTFE cannula provides enhanced chemical resistance. Use 4- or 8-channel manifold when dispensing into microplates.

Excellent ergonomics Soft plunger activation Versatile feeding through bottle, tubing or syringeNo need for consumablesSwift-set in-lab calibrationAutoclavable fully assembled at 121°C/250°FThree year warranty

Hand-held, self-refilling microdispenser intended for repeat distribution of microliter volumes. Carefully selected materials ensure durability and resistance to aggressive media. The Acura® manual 865 combines easy handling of a regular micropipette with the flexibility of a repeater pipette. There is no need for plastic tips or consumables, which may represent substantial savings.

Includes: 90 cm silicone tubing, SS blunt end cannula, female Luer inlet valve cap, Q.C. certificate and operating instructions.

•••••••

1

2

3

Inaccuracy (E%) Imprecision (CV%)Cat. No. Volume Division Min. Vol Mid. Vol. Max. Vol. Min. Vol Mid. Vol. Max. Vol. Qty/Case

W835001 5-50 μL 0.1 μL < ± 5.0% < ± 3.5% < ± 1.5% < 2.0% < 1.4% < 0.4% 1W835002 20-200 μL 0.2 μL < ± 2.5% < ± 1.8% < ± 1.0% < 1.5% < 1.0% < 0.3% 1W835004 100-1000 μL 1.0 μL < ± 1.5% < ± 1.1% < ± 0.6% < 0.6% < 0.4% < 0.2% 1

Performance values obtained with double distilled water at constant temperature (± 0.5°C) comprised between 20 and 25°C in accordance with EN ISO 8655.


W835012 Reservoir*, 30 mL, PTFE, Luer 1W835008 PTFE delivery cannula 1W835014 Manifold*, 4-channel, 20 mm spacing, Luer lock, stainless steel 1W835016 Manifold*, 8-channel, 9 mm spacing, Luer lock, stainless steel 1W851369 Work station 340 for 3 pipettes 1

*Autoclavable

1

2

3

See needles and cannulas on page 105.

NewNew

123


Socorex

Finger triggersSuction and dispensing are controlled through two ergonomically positioned finger triggers. They provide a comfortable grip with minimum activation effort.

Variable speedSwitch enables selection of fast or slow aspiration and dispensing (with blow-out). Fast speed filling up to 25 mL in under 3 seconds. Gravity dispense mode is designed for use with ’To Deliver’ (TD) pipettes.

Ordering information – InstrumentSupplied with charger, two spare hydrophobic filters (0.45 μm and 0.2 μm), bench / wall stand and instruction manual.

Battery chargeInstrument plug enables charging battery either in storage position or during use.

Convenient standSuitable for bench or wall mounting, stand houses Profiller™ electro with or without a pipette in place.

electroPowerful pump – fills 25 mL in 3 secondsSimple, fast operation Comfortable finger triggersLow battery lightNiMH battery rechargeable during useInterchangeable protection membrane filterStand suitable for bench or wall mountingOne year warranty

••••••••

This pipette controller fits any hand in a very comfortable way. The shape is ideal for working with glass or plastic pipettes within laminar flow hoods or microbiological safety cabinet. A powerful, yet quiet motor with a switch to set aspiration and dispensing speeds; this enables maximal working efficiency even with large pipetting volumes.


W851438 Profiller™ electro 446 1W851436 Spare bench stand for Profiller™ electro 446 1WI050950 Replacement 1.2V NiMH battery 1W851444 Fast charger and cord, USA plug 1W835022 Membrane filter, 0.45μm 5W851454 PTFE membrane filter, 0.2μm 5W835024 Silicone pipette holder 1W835026 Nose cone – white 3W835028 Alternative nose cones pink, violet, green (1 each) 3

NewNew

124Socorex


Protection filterInterchangeable hydrophobic membrane filter guarantees effective protection against airborne contamination during aspiration and dispensing.

Color coding conesNose cone can be changed for individual, laboratory or department identification. Choose among regular white or a bunch of three colors.

Pipette holderConical shape of silicone pipette holder tightly fits graduated glass or plastic pipettes. Autoclavable.

Squeezable bulbSimply squeeze large capacity silicone bulb for efficient aspiration. If additional aspiration is needed, it can be squeezed even during pipetting. 436 model has a distinct flat-shaped bulb.

Precise controlThumb lever button for left- and right-handed users to easily control both aspiration and dispensing speeds.

Liquid blow-outPress large button to expel residual liquid when using blow-out type pipettes.

manualLarge capacity bulbSoft action lever buttonEfficient blow-out systemPTFE protection membrane filterMaintenance freeAlternative color-coded nose conesOne year warranty

•••••••

Robust and easy to use, both models offer ergonomic shape and are lightweight. They provide smooth control in both aspirating and dispensing, using glass or plastic pipettes of volume up to 100 mL.


W835000 Profiller™ manual 435, egg style grey bulb 1W851434 Profiller™ manual 436, flat shaped blue bulb 1W835022 PTFE membrane filter, 0.45 μm 5W851454 PTFE membrane filter, 0.2 μm 5W835024 Spare silicone pipette holder 1W835026 Replacement nose cone - white 3W835028 Alternative nose cones pink, violet, green (1 each) 3

Dear Partner in Science,

Welcome to Socorex - a world of expertise in precision liquid handling.

Having our products in the new Wheaton catalog will mean a lot to you when it comes to choosing the best liquid handling products for your applications. Pages 99-106 and 111-124 will guide you through a wide selection of innovative, ergonomic and safe instruments, engineered and designed with excellence in mind for the most demanding scientists.

As an early leader, Socorex was the first manufacturer to obtain the EN ISO 9001 certification in this field, back in February 1991. This is only one of the many milestones having made total quality a company tradition and a cultural way-of-life for our management and staff members all together.

Wheaton, our exclusive agent in the U.S., is well trained and equipped to provide professional advice and technical support on all the Socorex products. And, all the Socorex products can be purchased through your local laboratory distributor.

Millions of Calibra® and Acura® pipettors and other famous Socorex instrument brands in use throughout the world would mean little, be it for our ever-growing number of customers and their proven satisfaction.

Thank you for your trust, we are committed to stay your quality partner in liquid handling.

Sylvain Christen, CEO

125


Benefits of Peristaltic PumpsNo contamination of fluid or the pumpSterile tubing means a sterile pumpNo tedious cleaning; fluid is confined to the tubeSelf-primingPump chemical compatibility is limited to the chemical compatibility of the tubing

Our pumps allow multiple dispensing of larger volumes and they can be used in a variety of applications – filling plates & tubes, transferring reagents, dispensing additive, and general filling applications. The Wheaton pump dispenser can replace a variety of other pumps in your lab due to its versatility and range of features. It can dispense solvents ranging in viscosity from organic solvents to medium syrups. The liquid being dispensed remain in the tubing, allowing for quick, non-contaminating changes and easy clean up.

Peristaltic Pumps…How they work

You can choose from two styles of Wheaton peristaltic pumps. These dispensing pumps can be used for filling applications in the laboratory and in the production area. When the pumps are in use, they are designed to prohibit contamination of the fluid or pump. To operate simply thread the tubing through the pump head and set your pump parameters. The pump is now ready to fill. The Wheaton pumps are easy to operate and cost effective. If you do need some help when setting and operating your pump, don’t hesitate to contact our Technical Service Department at 800.225.1437, or +1.856.225.1100. All pumps have a one year warranty.

•••••

The pump head squeezes the tubing. As the pump head continues its rotation, the tubing is able to recover its normal shape and size, drawing fluid into the tubing.

The pump head finally forces the fluid out of the tubing.

Fluid is being pushed along the tubing by the pump head.

Model Pump Omnispense® Plus Unispense® μP

Electrical 100 - 240 VAC, 50 / 60 Hz, 37 watts 120 - 240 VAC, 50 / 60 Hz, 140 watts

Operating Speed 41 - 417 RPM 100 RPM

Available Tubing Sizes 2, 3, 6, and 8 mm ID 2, 3, 6, and 8 mm ID

Dispense Ranges for Tubing Size:

2mm 1 - 2,400,000 mL — 3mm 1.5 - 3,300,000 mL 1.0 - 900 mL 6mm 2.2 - 10,500,000 mL 2.5 - 3,000 mL 8mm 3.5 - 17,000,000 mL 4.0 - 5,000 mL

Flow Rate Ranges for Tubing Size:

2mm 0.34 - 2.9 mL/sec — 3mm 0.67 - 5.9 mL/sec 2.0 mL/sec 6mm 2.19 - 19.6 mL/sec 9.0 mL/sec 8mm 3.34 - 29.1 mL/sec 18 mL/sec

Accuracy As high as ±1% midrange As high as ±1% midrange

Delay Time 0.5 to 25.5 seconds in automatic mode 0.5 to 60 seconds in automatic mode

Optional Pump Head Support

Stack up to 3 pump heads Stacking Pump Head for 2.3 mm Wall Tubing Pump Head for 1.6 mm Wall Tubing Stacking Pump Head for 1.6 mm Wall Tubing 5-channel Pump Head for Small-Bore Tubing with Fixed Stops

Weight 17 lb (7.7 kg) 11 lb (5 kg)

Dimensions with Pumphead (H x W x D) 12.75 x 7.75 x 15 inches 6.5 x 12.5 x 10 inches 32.4 x 19.7 x 38.1 cm 16.5 x 32 x 25.4 cm

126


Built-in constant flow rate dispenserMulti-language display promptsExternal connection for remote start/stopStore up to 100 settingsLightweight designAttach up to 3 pump headsSelf-prompting LCD displayConformance to UL, CSA and CE standards1 year warranty

The Omnispense Plus is a premium automatic dispenser known for its versatility and variable speed. This pump features a reverse-flow option for various dispensing and aspirating operations. It is perfectly suited for accurately dispensing liquids ranging in viscosity from aqueous solutions to medium syrups. The built-in constant flow rate dispenser is fully programmable for delivery of a preset volume over a specific period of time with a display of the cumulative volume delivered. With its lightweight design, this pump can be easily moved between work stations. Integrated tubing support conveniently holds the tubing for filling containers. The hand held tubing holder with a start and stop switch is excellent for remote filling or for use when working in a fume hood. The pump can accommodate operating speeds up to 417 RPM. There are bright, self-prompting LCD displays in English, French, German, Italian, Portuguese, or Spanish.

Pump is supplied with (1) 3 mm and (1) 6 mm ID Silicone tubing assembly. Each assembly is 5 feet (1.5 m) long with a borosilicate glass sinker on one end and a polypropylene nozzle on the other end. The silicone tubing assemblies are fully autoclavable.

•••••••••

SpecificationsElectrical ................................................... 100 - 240VAC, 50/60 Hz, 37 wattsOperating Speed .......................................................................41 - 417 RPM Available Tubing Sizes .................................................... 2, 3, 6, and 8 mm IDDispense Ranges for Tubing Size: 2mm ............................................................... 1 - 2,400,000 mL 3mm ............................................................ 1.5 - 3,300,000 mL 6mm .......................................................... 2.2 - 10,500,000 mL 8mm .......................................................... 3.5 - 17,000,000 mLFlow Rate Ranges for Tubing Size: 2mm ...............................................................0.34 - 2.9 mL/sec 3mm ...............................................................0.67 - 5.9 mL/sec 6mm .............................................................2.19 - 19.6 mL/sec 8mm .............................................................3.34 - 29.1 mL/secAccuracy ....................................................................As high as 1% midrange Delay Time ....................................................................... 0.5 to 25.5 seconds Optional Pump Head Support Stack up to 3 pump heads Stacking Pump Head for 2.3 mm Wall Tubing Pump Head for 1.6 mm Wall Tubing Stacking Pump Head for 1.6 mm Wall Tubing 5-channel Pump Head for Small-Bore Tubing with Fixed StopsWeight .......................................................................................17 lb (7.7 kg) Dimensions with Pumphead (H x W x D) .......................... 12.75 x 7.75 x 15 in 32.4 x 19.7 x 38.1 cm



Cat. No, Description Qty/Case

W375022 Optional Stacking Pump Head for 2.3 mm Wall Tubing 1W375023 Optional Pump Head for 1.6 mm Wall Tubing (tubing not available from Wheaton) 1W375024 Optional Stacking Pump Head for 1.6 mm Wall Tubing (tubing not available from Wheaton) 1W375025 Optional 5-Channel Pump Head for use with Small Bore Tubing with fixed stops (tubing not available from Wheaton) 1


Utilize the convenience of hands-free pump operation by installing the optional footswitch for the Omnispense Plus. This footswitch enables better control with its start and stop feature. This footswitch can remotely start and/or stop the dispense cycle when used with the Ominspense® Plus.


I050694 Footswitch 1

For a list of pump tubing and other accessories see pages 127-128.

Hand held tubing holder

127


Tubing Compatibility Silicone Viton®

Acids No Yes Bases No Yes Organic Solvents No Yes Sterile Fluids Yes Yes Concentrated Solvents No Yes Oils No Yes Low Temperature Yes Yes Autoclaving Yes No Gas Permeability High Low Other Characteristics Excellent biocompatibility Extremely chemical resistant

The tubing assembly consists of 1.5 m (5-foot) section silicone or Viton® tubing with a wall thickness of 2.3 mm and borosilicate glass sinker on one end and a polypropylene delivery nozzle on the other end.


Silicone Tubing Assembly, 2.3 mm wall thickness374310 2 mm ID, 5-ft section (1.5 m) 1374311 3 mm ID, 5-ft section (1.5 m) 1374313 6 mm ID, 5-ft section (1.5 m) 1374314 8 mm ID, 5-ft section (1.5 m) 1

Viton® Tubing Assembly, 2.3 mm wall thickness374315 2 mm ID, 5-ft section (1.5 m) 1374316 3 mm ID, 5-ft section (1.5 m) 1374318 6 mm ID, 5-ft section (1.5 m) 1374319 8 mm ID, 5-ft section (1.5 m) 1


Silicone Tubing, 2.3 mm wall thickness374304 2 mm ID, 25 ft (7.62 m) 1374305 3 mm ID, 25 ft (7.62 m) 1374306 6 mm ID, 25 ft (7.62 m) 1374308 8 mm ID, 25 ft (7.62 m) 1

Viton® Tubing*, 2.3 mm wall thicknessI054342 2 mm ID, 25 ft (7.62 m) 1 I054343 3 mm ID, 25 ft (7.62 m) 1 I054346 6 mm ID, 25 ft (7.62 m) 1 I054348 8 mm ID, 25 ft (7.62 m) 1

*Viton® tubing is for use with caustic reagents, organic solvents and scintillation cocktails. Viton tubing is sold in 1 foot (0.3 m) increments.

Accuracy as high as ±1% midrangeOperating speed fixed at 100 RPMCompact size; easily fits on bench-tops, in flow-hoods and cabinetsSelf-prompting LCD display1 year warranty

The Unispense μP is an accurate “easy-2-use” peristaltic pump that accommodates liquids ranging from aqueous solutions to medium syrups. With its small footprint, this pump fits well on bench-tops, in flow hoods and cabinets. Fluid is confined to the tube, therefore tedious cleaning is needless. Pump chemi-cal compatibility is limited to the chemical compatibility of the tubing. Set-up parameters are stored in memory for easy recall. The LCD display and one-step volume calibration ensure quick and easy setup. The pump operating speed is approximately 100 RPM. Pump is supplied with (1) 3 mm and (1) 6 mm ID Silicone tubing assembly. Each assembly is 5 feet (1.5 m) long with a borosilicate glass sinker on one end and a polypropylene nozzle on the other end. The silicone tubing assemblies are fully autoclavable.

SpecificationsElectrical ................................................120 - 240 VAC, 50 / 60 Hz, 140 watts Operating Speed .............................................................................. 100 RPM Available Tubing Sizes......................................................... 3, 6, and 8 mm IDDispense Ranges for Tubing Size:

3mm ...................................................................... 1.0 - 900 mL 6mm ................................................................... 2.5 - 3,000 mL 8mm ................................................................... 4.0 - 5,000 mL

Flow Rate Ranges for Tubing Size:3mm ......................................................................... 2.0 mL/sec 6mm ......................................................................... 9.0 mL/sec 8mm .......................................................................... 18 mL/sec

Accuracy ................................................................. As high as ±1% midrange Delay Time ..............................................0.5 to 60 seconds in automatic mode Weight ...........................................................................................11 lb (5 kg) Dimensions with Pumphead (H x W x D) .....................6.5 x 12.5 x 10 inches ......................................................................................16.5 x 32 x 25.4 cmCat. No. Voltage Qty/Case

Unispense® with Standard Pump Head374301 110 VAC 1374302 220 VAC 1

Utilize the convenience of hands free pump operation by installing the optional footswitch for the Unispense. This footswitch enables better control with its start and stop feature. This footswitch can remotely start and/or stop the dispense cycle when used with the Unispense® Pump Dispenser.


I050694 Footswitch 1

•••••

128



374320 Small Glass Sinker (fits 2 and 3 mm ID tubing) 6374321 Large Glass Sinker (fits 6 and 8 mm ID tubing) 6374322 Hose Barb/Luer Adapter for attaching manifold (2 mm and 3 mm) 1374324 2 and 3 mm Polypropylene Nozzle 6374326 6 mm Polypropylene Nozzle 6374328 8 mm Polypropylene Nozzle 6

Optional tubing support conveniently holds the tubing in place for filling operations.


I054106 Tubing Support Stand 1

For use with Unispense® and Omnispense® Plus Pump Dispensers

Cat. No. 374325 is comprised of silicone tubing with female Luer and male Luer lock fitting on opposing ends. Sterile, individually packaged. Length: 42” (106.7 cm). (Vented spike not included).

Cat. No. 374330 is comprised of high volume silicone and PVC tubing with vented spike and drip chamber and male Luer lock fitting on opposing ends. Sterile, individually packaged. Length: 5 ft. (1.5 m).


374325 3 mm I.V. Additive Pump Chamber Assembly 10374330 6 mm I.V. Additive Pump Chamber Assembly 10

•

Glass Sinker Hose Barb/Luer Adapter Polypropylene Nozzle

For a complete selection of Roller Culture Apparatus see pages 58 - 63 of the Cell Culture section. For Glass Roller Bottles, see page 15 of the Bottle section.

Bench Top Rollers page 58

Incubators page 64

R2P pages 62 - 63

•

•

•

Cat. No. 374325 Cat. No. 374330

129


Ideal for sub-surface liquid samplingStainless steel 500 mL capacity

These units are constructed of corrosion resistant Type 302 stainless steel. A weighted plunger with a Viton® “O” ring seals the reservoir chamber at the bottom. Use a cable to lower sampler to desired depth. A negligible amount of liquid will enter during descent. Pull second cable to operate the sampling valve and release to reseal the chamber. This unit is not supplied with cables. We recommend use of a cable that can support 50 pounds or more and is made from stainless or Teflon® coated stainless steel.

Cat. No. Capacity (mL) Dia x Length Qty/Case

885200 500 2.5 x 10 in (6.35 x 25.4 cm) 1

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W heaton offers a variety of sampling devices for industrial and QC applications, environmental

compliance hazardous/toxic materials evaluation and site evaluation/remediation work. Sampling devices are offered for liquids, solids, sludges, earth/soil, water, etc. They are ideal for sampling from barrels, drums, water reservoirs, sub-surface effluents and are efficient and easy to use. Most of Wheaton’s sampling devices help meet EPA standards and regulations.

Easy to useDisposable or reusable units availableLiquid-tight Teflon® seal eliminates sample leakageTeflon® seal design permits sampling to within 1/2” of drum bottom

Glass Coliwasa Samplers are used to collect composite liquid samples from top to bottom in drums, tanks and reservoirs. After a sample has been collected, lower and press lightly on the inner tube, which positively seats the Teflon® seal, locking the sample column into place. This unit has a tapered bottom with an approximate diameter opening of 1/2”, (1.3 cm) and a sample capacity of 200 mL. Prescored samplers make disposal easier.

Dimensions: Diameter 7/8” x Length 42”, (2.25 cm x 106.5 cm)

Cat. No. Description Material Qty/Case

Disposable Unit885220 Prescored Soda-Lime Glass 12

Reusable Unit885230 Unscored Borosilicate Glass 12885231 Prescored Borosilicate Glass 12

Replacement Seals885232 Replacement Seals Teflon® 10

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Disposable Glass UnitsOpen ended

These soda-lime glass units are 43” long and can be easily broken for quick disposal. To use simply insert one end of the drum thief into a drum and hold a finger over the opening on the other end. This will trap liquid in the tube. Carefully remove the drum thief and empty the contents into a testing container. Drum thieves are ideal for quick and easy sample retrieval.

Cat. No. Approx. Capacity (mL) Dia (mm) Qty/Case

885300 25 7.5 100885302 75 12 24885303 150 18 24

Ideal for sampling unknowns from drums and tanks

Manufactured from polypropyleneProvided with 125 mL sample bottle

The E-Z Sampler is an efficient and time saving method of sampling unknowns from drums and tanks. Constructed of chemically resistant polypropylene, the E-Z Sampler has a 39" (100 cm) head/shaft assembly and is supplied with a 125 mL borosilicate glass bottle with a Teflon® lined cap. The filling ports are located above the bottle to ensure

that it is completely filled to reduce the possibility of air entrapment. The bottle has a 33-430 screw thread finish.


885020 Complete Unit with bottle 1885025 125 mL Bottle, with Cap 12240480 Replacement Cap 100

Ideal for sampling from spillways, docks and other sub surfaces

No need to physically enter the sample area

Eliminates surface contaminationHelps meet EPA requirements for water

and wastewater samplingLightweight construction

This unit affords you easy access to samples from spillways, docks, etc., without having to physically enter the sample areas. It is

constructed of 72” x 3/4” (183 x 2 cm) square aluminum tubing with a golden anodine finish. It comes with a 1000 mL narrow-mouth sample bottle with a PTFE-lined cap and clamps for large and small bottles.


990250 Sub-Surface Grab Sampler I 1990477 Replacement Sample Bottle, 1000 mL, 38-430 1240481 Black Phenolic Replacement Cap, 38-430, with PTFE liner 100

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Use for sampling sludge or semi-solid materialDisposable glass

This disposable soda-lime glass Coliwasa is used to sample sludge or semi-solid material. The rigidity of the glass makes it suitable for boring through semi-solid sludge-type materials. The tight Teflon® seal keeps in liquid and sludge material. The outer tube is 40 inches (101.6 cm) long.


885235 200 mL capacity 12885236 Replacement Inner Rod with Teflon® Seal 12

Clear PVCPositive seal for sample retentionSampling can be achieved up to 20 feet

The uniquely designed top spin bar and bottom valve plate mechanism provides a positive seal for sample retention along with greater control for releasing the sample. The modular design also adds flexibility by enabling the connection of several sections to form a longer sampling unit up to 20 feet (610 cm). To use the Coliwasa, simply turn the bar on the top of the handle, insert slowly into the drum to collect liquid in the column, pull the top handle to seat the bottom valve plate, and spin the top handle closed, trapping the liquid in the sampler. This Coliwasa comes with a 48” long cleaning brush.

Dimensions (ID x OD): 1 3/8 x 1 5/8 in (3.5 x 4.12 cm).

Cat. No. Description Approx. Volume (mL) Qty/Case

Coliwasa Unit885250 4 ft. (122 cm) Coliwasa Unit 760 1885252 6 ft. (183 cm) Coliwasa Unit 1100 1

Extension and Connector885253 4 ft. (122 cm) Extension and Connector 760 1885255 6 ft. (183 cm) Extension and Connector 1100 1

Replacement Shaft885256 Replacement 4 ft. (122 cm) PVC Shaft for 885250 — 1885257 Replacement 6 ft. (183 cm) PVC Shaft for 885252 — 1885258 Bottom Plate and SS Nut — 1

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Use when sampling hazardous and corrosive materials, as well as wastewaterChemically resistant stainless steel and polypropylene constructionWide mouth accepts light sludges and slurriesModular design uses 6’ add-on sections up to 24’ in length

This unit works on the same principle as the Grab Sampler II. The Grab Sampler III fits wide-mouth bottles with a 70-400 screw thread finish. A displacement plunger allows the collection of samples in a 1000 mL bottle with approximately 1 inch of head space between the sample and the underside of the bottle closure. The Grab Sampler III includes a safety-coated 1000 mL bottle and a green urea cap with a Teflon® liner. An extension kit (990471, sold separately) includes a 6 feet stainless steel extension shaft, coupling and connecting cable. To use it, you pull a cable that lifts a spring-loaded plunger, allowing liquid to enter through four 5/8” x 1-1/2” slots. Release the cable when the bottle is full to reset the plunger.


990470 Sub Surface Grab Sampler 1990471 6 ft. (183 cm) Extension Kit 1216641 Safety Coated Sample Bottle, 32 oz., 70-400 (without cap) 12239244 Polypropylene Replacement Cap, 70-400, with PTFE liner 48

For use with Sub Surface Grab Sampler I & II1000 mL narrow mouth borosilicate glass bottle38-430 screw cap with PTFE linerAutoclavable

This bottle is used with the Sub Surface Grab Samplers I and II and has a 38-430 screw thread finish with a PTFE lined cap. The bottle has a hand grip for easy handling.


990477 1000 mL Bottle with Cap 1

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Excellent for accessing fluid flow in deep manholes and hard to reach sub-surface sampling areasHelps meet EPA requirements for water and waste water samplingAvailable in lengths up to 18 ft. (5.5 m)

Each sampler consists of an inert polypropylene head with stainless steel fittings and an aluminum pole assembly with a golden alodine finish. It comes with a 1000 mL narrow-mouth sample bottle with a PTFE-lined cap. To use it, screw a sample bottle into the head and lower to the desired depth. Pull the cable at the top of the handle which will lift a spring-loaded plunger from the bottle opening, allowing liquid to enter through four 5/8” diameter holes. Release the cable when the bottle is full to reseat the plunger.


990350 6 ft. (183 cm) Sub Surface Grab Sampler II 1990400 12 ft. (366 cm) Sub Surface Grab Sampler II 1990450 18 ft (549 cm) Sub Surface Grab Sampler II 1990477 Replacement Sample Bottle, 1000 mL, 38-430 1240481 Black Phenolic Replacement Cap, 38-430, with PTFE liner 100

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See replacement caps on page 13.

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For use with Sub Surface Grab Sampler III1000 mL wide mouth borosilicate glass bottle70-400 screw cap with PTFE linerAutoclavable

These wide mouth sample bottles can be repeatedly autoclaved and come with PTFE-lined caps.


990476 1000 mL Bottle with Cap 1

This opener can be used with hazardous materials. It is cast from lightweight, durable aluminum alloy and features a quarter-turn opening action.


885295 Drum Opener 1

This scoop is made from 302 stainless steel and is ideal for sampling powders, slurries, soils, etc.

Dimensions: Overall length is 9-1/2" (24 cm); the bowl depth is 1" (2.54 cm).


885540 Scoop 4

Used for extracting a plug from materialManufactured from 304 stainless steel

This sampler is made from 304 stainless steel and is tapered for extracting a plug of material from solids (soil, clay) or semi-solids (grease, sludge). Dimension: Length 7" (17.75 cm).


885380 Trier Sample 1

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See the Chemistry Glassware Section on pages 65 - 82 for environmental testing apparatus.

Arsine Generators page 67

Cyanide Distillation Kits pages 67 - 68

Diazomethane Generators pages 68

Evaporative Concentrators pages 68 - 69

Dessicators pages 73-74

Filtration Apparatus pages 75 - 81

Imhoff Cone for Solid Determination page 82

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For a complete selection of sample collection containers see the Bottle Section on pages 3-30, and the Vial section on pages 139-154.

Plastic Bottles pages 22-30

EPA Vials page 142

Vials in Lab File page 150

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Customized Services

Code 39Code 128Interleaved 2 of 5Linear Bar Codes2 Dimensional (2D)Data Matrix

Laser: Direct product markedCeramic: Imaged ceramic label applied and firedPhoto Imaging: Ultra high-density graphically

perfect imagesFlexographic: Ink based static printing and label

convertingDigital: Full process color 1800 dpi, variable

data

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W hile our heritage is in manufacturing quality products, today we provide services that streamline your operations. It’s easier than ever to get your laboratory products customized the way you need them.

We have integrated a suite of services that you can select from to ensure you obtain the right solution. Each customer is different and each project unique-so each solution is modified to meet individual needs and requirements. Quality products are what we are known for and now with SciLutions®, we bring a whole new level of services to your doorstep.

Made to orderEngineering and design support Custom packaging Custom cut glass tubing and rodsArtwork/decal application

Request a specific packaging method or a completely unique container based on your specific require-ments for dimension, shape, and material. Need a custom glass vial? We’ve been producing specialty vials like V Vials® and the E-Z Ex-Traction® high recovery vials for years following the precise instruc-tions of our customers.

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Uniquely numbered and bar coded to fit your application100% scannableWide range of symbologiesPackaged and sequentially numbered to your specifications

Bar coding can help you identify and track anything in your lab or compound storage facility. Increase the speed of data collection while eliminating errors and ultimately reducing costs. It is imperative that bar codes are read correctly every time, stay adhered to the container, and are durable enough to withstand severe environments.

We utilize a variety of symbologies and materials to provide unique identification solutions for biotech, pharmaceutical, and compound storage facilities. Bar coding techniques are available from simple pressure sensitive labels to direct marked containers.

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Extrusion Blow MoldingInjection Blow MoldingInjection MoldingInjection Stretch Blow MoldingTubular Vial Conversion

Molded Glass

High Density Polyethylene (HDPE)Low Density Polyethylene (LDPE)Polyethylene Terephthalate (PET)Polypropylene (PP)Cyclo-Olefin Copolymer (COC)Type I Borosilicate GlassType IIl Soda-Lime Glass

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When needed, an expert team of corporate engineers, R&D professionals and integration specialists will work collaboratively with you to achieve a solution that meets your needs.

We take care of the details. We will handle all of the in-between steps to ensure your process is smooth and efficient. Most of all, we want to meet your particular requirements and specifications from the beginning to the end.

SciLutions is about simplifying your supply chain and optimizing your operations. We can work collaboratively on long term solutions and ensure they are achieved. It’s not about one order or one delivery. It’s about ensuring our products arrive ready to be used, exactly the way you want them.

134Customized Services


Pre-weighed containersTraceability documentationAccuracy to ±0.2 milligrams

Part of the SciLutions® program is to provide you with pre-weighed containers. Taring ensures that the amount of material in the container can be accurately measured at any given time. Wheaton offers this service, eliminating the ex-tra step of pre-weighing in the lab. Weights for each container are recorded to the accuracy of ±0.2 milligrams. The results are summarized in the form of a spreadsheet allowing for each and every container to be fully traceable.

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Trace organic analysisStorage of materials prone to adhering to glassExtraction glassware

Surface treatment refers to a process by which the inside of a container is deactivated via silanization or siliconization. The coating provides a protective barrier between

the contents and the glass, eliminating active sites on the glass that could poten-tially react with its contents.

We treat vials and vial inserts using vapor phase deposition for silanization and siliconization coating to lubricate and protect against alkalinization. This service is ideal for serum vials and pH sensitive storage. Certificates of conformance are

always available based on customer needs and requirements.

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Low particulate cleaningDepyrogenationChemical cleaning for trace analysisSterilization (E-beam, Gamma Irradiation, Autoclaving)Water for Injection (WFI) and USP rinsesTotal Organic Carbon (TOC) process

Depending on your application, you may require any number of “levels of clean.” To ensure we can accommodate any request, we employ the most sophisticated methods to remove trace organics, trace inorganics, volatile organics, and organic carbon residues.

We use USP purified water, HEPA filtering, and package items in certified class 100/10 cleanrooms. In addition, we can deliver our glass containers and accessories with endotoxin levels of less than 0.06 EU/mL with full documentation and certification.

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Certificate of Analysis, TOCCertificate of Analysis, EndotoxinCertificate of Analysis, ParticlesCertificate of ProcessingCertificate of Sterility/USPCertification of ComplianceSelected Testing by USP MethodsMaterials Certification

Individual or multi-unit cleanroom bagsSingle or double baggedAutoclaved bagging in cleanroom bags

(closures only)Autoclaved bagging

(single, double, or triple bagged)

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W heaton offers a variety of staining dishes and Coplin jars suitable for various

histology staining. Routine staining is performed to give contrast to the tissue being examined, as without staining it is very difficult to see differences in cell morphology. Hematoxylin and eosin (abbreviated H&E) are the most commonly used stains in histology and histopathology. Hematoxylin colors nuclei blue, eosin colors the cytoplasm pink. To see the tissue under a microscope, the sections are stained with one or more pigments. There are hundreds of various other techniques which have been used to selectively stain cells and cellular components. Other compounds used to color tissue sections include Safranin, Oil Red O, Congo red, Fast Green FCF, Silver salts and numerous natural and artificial dyes that were usually originated from the development dyes for the textile industry.

Histochemistry refers to the science of using chemical reactions between laboratory chemicals and components within tissue. A commonly performed histochemical technique is the Perls Prussian blue reaction, used to demonstrate iron deposits in diseases like Hemochromatosis.

Histology samples have often been examined by radioactive techniques. In historadiography a slide (sometimes stained histochemically) is X-rayed. More commonly, autoradiography is used to visualize the locations to which a radioactive substance has been transported within the body; such are cells in S phase (undergoing DNA replication) which incorporate tritiated thymidine, or sites to which radio labeled

nucleic acid probes bind in in situ hybridization. For autoradiography on a microscopic level the slide is typically dipped into liquid nuclear tract emulsion, which dries to form the exposure film. Individual silver grains in the film are visualized with dark field microscopy.

Recently, antibodies are used to specifically visualize proteins, carbohydrates and lipids: this is called immunohistochemistry, or when the stain is a fluorescent molecule, immunofluo-rescence. This technique has greatly increased the ability to identify categories of cells under a microscope. Other advanced techniques can be combined with this, such as nonradioac-tive in situ hybridization to identify specific DNA or RNA molecules with fluorescent probes or tags that can be used for immunofluorescence and enzyme-linked fluorescence amplification (especially alkaline phosphatase and tyramide signal amplification). Fluorescence microscopy and confocal microscopy are used to detect fluorescent signals with good intracellular detail. Digital cameras are increasingly used to capture histological and histopathological images.

Wheaton staining dishes, covers, and racks are manufactured from soda-lime glass that resists staining from Eosin or Hematoxylin. Staining dishes are available in many configurations to accommodate up to 50 slides, with various glass and stainless steel racks. Coplin jars can be used for pre-staining with an alcohol buffer. Slides are held vertically for easy manipulation. A Coplin jar can also serve as a preparation chamber for micro-thin-layer chromatography.

Reference from: Michael H. Ross, Wojciech Pawlina, (2006). Histology: A Text and Atlas. Hagerstown, MD: Lippincott Williams & Wilkins. ISBN 0-7817-5056-3.

Stain Common use Nucleus Cytoplasm Red Blood Cell (RBC) Collagen Fibers Specifically stains

Hematoxylin General staining Blue N/A N/A N/A Nucleic acids - Blue when paired with Eosin blue eER (ergastoplasm) - Blue

Eosin General staining N/A Pink Orange/Red Pink Elastic fibers - Pink when paired with Hematoxylin reticular fibers - Pink

Toluidine blue General staining Blue Blue Blue Blue Mast cells granules - PurpleMasson’s trichrome stain Connective tissue Black Red/Pink Red Blue/Green Cartilage - Blue/green

Muscle fibers - RedMallory’s trichrome stain Connective tissue Red Pale Red Orange Deep Blue Keratin - Orange

Cartilage - Blue Bone matrix - Deep Blue Muscle fibers - Red

Weigert’s elastic stain Elastic fibers Blue/Black N/A N/A N/A Elastic fibers - Blue/BlackHeidenhains’azan Distinguishing cells from Red/Purple Pink Red Blue Muscle fibers - Redtrichrome stain extracellular components Cartilage - Blue

Bone matrix - BlueSilver stain Reticular fibers, Nerve fibers N/A N/A N/A Brown/Black Reticular & Nerve Fibers - Brown/BlackWright’s stain Blood cells Bluish/Purple Bluish/Gray Red/Pink N/A Neutrophil granules - Purple/Pink

Eosinophil granules - Bright Red/Orange Basophil granules - Deep Purple/Violet Platelet granules - Red/Purple

Orcein stain Elastic fibres Deep Blue N/A Bright Red Pink Elastic fibres - Dark Brown Mast cells granules - Purple Smooth Muscle - Light Blue

Periodic (PAS) Basement Membrane, Blue N/A N/A Pink Glycogen and otheracid-Schiff stain Localising carbohydrates Carbohydrates - Magenta

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10-20 Slide UnitThis 20-slide unit is the standard for manual staining procedures. The removable glass slide rack has an open bottom to facilitate rapid immersion and drainage, reducing carryover. The rack holds 10 single slides, 19 slides arranged alter-nately straight across and diagonally, or 20 slides back-to-back of standard size: 3” x 1” (75 x 25 mm), 3” x 1-1/2 “ (75 x 38 mm) and 3” x 2” (75 x 51 mm) sizes. Manufactured from soda-lime glass. Approximate inside dimensions: 95 mm L x 76 mm W x 64 mm D.


900200 Complete (Dish, Cover, Rack, Handle) 6900201 Dish 3900202 Cover 3900203 Dish and Cover 3900204 Glass Slide Rack 3900205 Handle 6

10-20 Slide UnitDesigned for staining 3” x 1” (75 mm x 25 mm) microscope slides. Holds 10 single slides, or 19 slides arranged alternately straight across and diagonally, or 20 slides back to back. Approximate inside dimensions: 80 mm L x 55 mm W x 35 mm D.


900170 Staining Dish, with Cover 6

16-40 Slide Unit These mix-and-match components offer greater flexibility in meeting your requirements. This staining dish accommodates 16, 20 and 30 slide racks. It holds slides sizes 3” x 1” (75 mm x 25 mm), 3” x 1-1/2 “ (75 mm x 38 mm), and 3” x 2” (75 mm x 51 mm). Manufactured from soda-lime glass. Approximate inside dimensions: 118 mm L x 88 mm W x 70 mm D.


900303 Dish and Cover 6900301 Dish 3900302 Cover 3900234 30-Slide Rack Stainless Steel with hinged handle 3900254 (16-32) Slide Rack, Glass 3900304 (20-40) Slide Rack, Glass 3

50-Slide UnitThis slotted rack holds 50 microscope slides, sizes 3” x 1” (75 mm x 25 mm), 3” x 1-1/2” (75 mm x 38 mm), and 3” x 2” (75 mm x 51 mm). The rack is made of non-tarnishable stainless steel that is resistant to staining solutions. The handle is permanently attached, but hinged to permit closure of the dish and easy insertion and removal of the microscope slides. The dish is manufactured from soda-lime glass. Approximate inside dimensions: 185 mm L x 88 mm W x 68 mm D


900400 Complete (Dish, Cover, and Rack) 6900401 Dish 3900402 Cover 3900403 Dish and cover 3900404 50-Slide Stainless Steel Rack, with Handle Attached 3

8-16 Slide Unit Fits 8 single slides or 16 slides back-to-backUsed as staining dish or TLC developing chamberIncludes glass lid

This Hellendahl-type dish can be used for staining or as a TLC developing chamber. It holds 8 individual 3” x 1” (75 mm x 25 mm) slides or, if necessary, 16 slides back-to-back. Manufactured from soda-lime

glass. Approximate inside dimensions (mm): 76 L x 25 W x 75 D


900630 Staining Dish 6

Unit holds 4 cover slipsPolyproplene screw cap with PTFE coated PE liner

These staining jars hold up to 4 cover slips 17-23 mm wide, and up to 30 mm long. Longer cover slips can be accommodated if the cap is removed. The jar includes a 43-400 white polypropylene screw cap with a PTFE coated polyethylene liner. Made from soda-lime glass, the jar is chemical resistant. In addition to staining applications, Columbia Jars can be used for cleaning cover slips, as well as coating them with materials such as poly-lysine or silane.


W900180 Columbia Jar & Cap 1W900180-6 Columbia Jar & Cap 6

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8-10 Slide UnitFits 8 single slides or 16 slides back-to-backWide top makes transferring slides convenientIncludes glass lid

This jar’s wide top is designed for convenience in transferring slides, making it especially suitable for staining slides that are inscribed on one end. The jar holds 8 single 3” x 1” (75 mm x 25 mm) slides vertically or 16 slides back-to-back. Manufactured from soda-lime glass. Approximate inside dimensions (mm): 42 L x 26 W x 85 D.


900620 Staining Jar with Cover 6

Polypropylene grip allows for easy and safe transfer of five slides to other containers for staining. It fits into Wheaton Coplin staining jars.


900575 Slide Grip 2

Ideal for Boerner-Jones-Lukens microfluocculation test

Manufactured from USP Type III glass

This slide is used for procedures involving 0.01 to 0.15 mL of fluid. The slide has 10 cells in two rows of five each. Each cell is 2.5 mm

deep and is designed to produce flattened drops of uniform depth. These reusable slides are manufactured from Wheaton 810 glass.

Cat. No. Description Dimensions L x W (mm) Qty/Case

798550 Boerner Micro Test Slide 109 x 58 12

This bottle, manufactured from soda-lime glass, is ideal for applying mounting media. It comes with a glass applicator rod and a glass cap, which is ground to the shoulder of the bottle to form a seal.

Cat. No. Size (mL) Dia x H (mm) Qty/Case

208890 100 75 x 100 6

Glass cap is used to reduce evaporation of alcohol when not in use

This burner, manufactured from soda-lime glass, is designed for use with isopropyl or denatured ethyl alcohol. Its low-heat flame is well suited for microscopy purposes. The unit is supplied with a ground glass stopper. The reservoir holds 100 mL of alcohol.


237070 Alcohol Burner 75 x 100 6

Replacement Parts237071 Wick, 0.25" Dia x 6" L — 25 237072 Cork Stopper — 10

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5-10 Slide UnitHolds 10 slides Polypropylene screw cap55 mL capacity

This unit holds ten 3” x 1” (75 mm x 25 mm) slides, which extend above the opening so you can manipulate them without using forceps. The screw cap is made of linerless white

polypropylene to reduce solvent evaporation and spills during storage. The unit has a rectangular base and holds approximately 55 mL. Manufactured from soda-lime glass. Approximate inside dimensions: 26 mm L x 26 mm W x 70 mm D.


900520 Coplin Jar with PP Screw Cap 6900522 Replacement Cap, 58-400 6

5-10 Slide Unit, with Screw Cap

Unit holds 5 slides vertically or 10 slides back-to-back

Polyproplene screw cap60 mL capacity

This unit is used for staining slides, or as a develop-ing chamber for thin-layer chromatography. It holds five single 3” x 1” (75 mm x 25 mm) slides vertically, or 10 slides back-to-back. The screw cap is made

of linerless white polypropylene to reduce solvent evaporation. The unit has a rectangular base and holds approximately 60 mL. Manufactured from soda-lime glass. Approximate inside dimensions: 26 mm L x 26 mm W x 90 mm D.


900570 Complete 6900522 Replacement Cap, 58-400 6

5-10 Slide UnitUnit holds 10 slidesGlass Cover65 mL capacity

This popular staining jar has heavy glass walls and a broad base for increased stability. It holds five single 3” x 1” (75 mm x 25 mm) slides vertically, or 10 slides back to back, and requires low reagent volume (approximately 65 mL). Manufactured from soda-lime

glass. Approximate inside dimensions: 26 mm L x 26 mm W x 90 mm D.


900470 Coplin Jar with Glass Cover 6

5-10 Slide Unit Fits 5 single slides or 10 slides back-to-backExtra tall lid accommodates 100 mm slides

The chamber is designed with an extra tall lid to accept 5 single 25 mm x 75 mm and 25 mm x 100 mm slides or 10 slides back to back. Manufactured from soda-lime glass.Approximate inside dimensions (mm): 26 L x 26 W x 90 D.


276840 Staining Jar with Lid 6

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138Product Application Notes


AbstractCoating slides with Poly-Lysine allows binding of DNA and certain proteins. A Poly-Lysine coating also promotes cell adhesion to slides for culturing or experiments, such as calcium ratio imaging. Wheaton staining dishes offer a convenient method for efficiently coating a number of slides.

Materials and MethodsMaterial Quantity Cat. No.

30-Slide Rack, Stainless Steel 2 900234Staining Dish & Cover 4 900303Poly-Lysine solution (0.1% w/v) 70 mL Available commerciallyTissue Culture PBS 70 mL — Double-Distilled water 2 L — Amber HDPE bottles 1000 mL 209130Drying Oven* 1 — Orbital shaker 1 — Centrifuge 1 — Dust-free plastic containers to hold slides —

*45°C - vacuum oven preferred

ProcedurePrepare the poly-lysine by adding 70 mL of

poly-lysine to 70 mL of PBS solution and 560 mL water. Store in an amber HDPE bottle (209130).

Clean slides as described in the Slide Cleaning Product Application Note. Place an equal number of slides in each of two stainless steel slide racks.

Transfer slides to the polylysine solution and shake for 15 minutes to 1 hour.

Transfer the racks of slides to fresh staining dishes filled with fresh double-distilled water. Plunge up and down at least 5 times to rinse the slides.

Centrifuge slides on a microtiter plate carrier at 500 RPM for 5 minutes. Place paper towels under racks to absorb moisture. Use two racks of slides to balance the centrifuge.

Place the racks of slides in clean staining dishes with covers for transport to vacuum oven. Place the dishes in the oven at 45°C for 10 minutes. Vacuum is optional.

Store the slides in a plastic slide box without a rubber mat bottom.

Numerous users commented that slides should be stored for 14 days before spotting DNA. Slides are best when used within 4 months.

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AbstractClean microscope slides are important for all areas of microscopy. Even pre-cleaned slides can carry a significant amount of oil or lint, even from a freshly opened box.

Wheaton staining dishes offer a convenient method to efficiently clean a number of slides as an initial preparation for coating slides or for microscopy.


Glass Microscope Slides 60 — 30-Slide Rack, Stainless Steel 1 900234Staining Dish & Cover 2 900303Double Distilled Water 5 liters — Solid NaOH 70 g — 95% Ethanol 420 mL — Drying Oven* 1 — Orbital Shaker 1 —

*45°C-vacuum oven preferred

ProcedurePrepare the cleaning solution by dissolving 70 g

NaOH in 28 mL double distilled water. Add 420 mL 95% ethanol. If the solution is cloudy, add double distilled water until the solution clears.

Place the slides in a stainless steel rack and the rack in the staining dish. Pour the cleaning solution from Step 1 over the slides so the slides are covered. Cover the staining dish with a glass lid, and put the staining dish with slides on the orbital shaker to mix for 2 hours.

Transfer the slides to a fresh staining dish filled with double distilled water. Rinse the slides by plunging them in the water several times. Repeat the rinse at least 4 times, using fresh double distilled water for each rinse. It is critical to remove all traces of Ethanol-NaOH.

If you are not coating the slides, place the slides in a slide rack in an oven at 45°C. Use of a vacuum oven is optional. Protect the slides from dust in the air by rinsing all glassware prior to use, and by keeping all solvents, slides and staining dishes covered.

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AbstractSilane coatings are used for paraffin sections, frozen sections and in situ hybridization. A quick & easy procedure is provided to coat microscope slides with a silane coating.


Staining Dish & Cover 3 90030320 Slide Rack, Glass 3 9003042% 3-aminopropyltriethoxysilane (AES) in acetone* 500 mL — Double-Distilled water 500 mL — Acetone 500 mL —

*Keep this in a fume hood

ProcedurePrepare 2% 3-aminopropyltriethoxysilane (AES) in

acetone (do this under a fume hood).

Clean slides as described in the Slide Cleaning Product Application Note.

Place slides in a slide rack, and rinse slides in a staining dish with acetone. Move the rack of slides to a staining dish containing 2% AES in acetone. (Note - dedicate this staining dish for the AES solution. Do not use it for anything else). The slides will need 5 to 15 minutes in this solution.

Rinse slides by dunking 4 times in fresh acetone. Repeat this wash step 2 times with fresh acetone.

Rinse slides by dunking them into a staining dish containing fresh double distilled water.

Dry slides overnight in a dust free environment. They can be dried in a vacuum oven. These slides can be kept for up to 6 months in a dust-free plastic box.

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W heaton Science Products offers the most complete line of vials and racks available to the

laboratory market. Wheaton sample vials, V Vials® and liquid scintillation vials are the original and standard by which vials are judged.

Wheaton sample vials, fabricated from glass tubing, offer uniform sidewall and bottom thickness. In addition, they are lightweight and have excellent strength and clarity. Our sample vials are available in both clear and amber glass and have a variety of uses ranging from sample storage to freeze drying.

Our scintillation vials are the original scintillation vials invented by Wheaton over 50 years ago. Manufactured from low potassium glass, polyethylene terephthalate and high density polyethylene, the Wheaton line of vials is the largest and most diverse in the industry.

The new Wheaton E-Z Ex-Traction vial is designed for automated compound management systems and the V Vials are designed for reaction and sample storage. The unique conical interior bottom of these vials ensures maximum security and economy in handling, storage and shipping of costly micro and semi-micro samples.

When you are looking for a high quality and dependable vial, Wheaton can provide the product for your application. Browse through the following pages to select the vial that meets your requirement.

Autosampler ..................................................... 83-96

Ampules ...............................................................1-2

Cryogenic ..................................................... 140-142

Culture ................................................................ 142

Dilution ................................................................ 142

E-C Sample Vials .......................................... 151-152

EPA ..................................................................... 142

E-Z Ex-Traction® .................................................. 143

High Recovery .............................................. 143-145

Lab Files™ .................................................... 150-151

Liquid Scintillation ........................................ 146-148

Micro Sample ...................................................... 148

Mini-File® ............................................................ 153

M-T Vial File® ............................................... 152-153

NextGen™ V Vial® .......................................... 144-145

Omni-Vial® .......................................................... 147

Racks .................................................................. 149

Sample ......................................................... 150-154

Sampule® ............................................................ 148

Serum ................................................................... 20

Vacule® ............................................................... 154

Vial File® ....................................................... 152-153

V Vial® .......................................................... 144-145

139


140Cryogenic


Wheaton cryogenic vials, caps, vial racks, and freezer boxes are designed for simple identi-fication and effortless handling. With Wheaton plastic Cryules®, both the closures and tubes are manufactured from medical-grade polypropylene with the same coefficient of expan-sion; this further enhances the leak proof qualities of these vials at changing temperatures.

All Wheaton Cryules and racks are autoclavable up to 121°C. When autoclaving plastic Cryules, the cap must be loosened or removed to allow pressure equalization upon cooling; this prevents pressure build-up within the vial.

The Wheaton glass Cryules are made from Wheaton 33 low extractable borosilicate glass, (that conforms to ASTM Type I, Class A and USP Type I requirements) which can be hermetically sealed.

Use caution; do not use cryogenic containers for storage directly in contact with the liquid phase of liquid nitrogen; use in gas phase only. Always use appropriate safety equipment such as gloves, face shields, and safety cabinets to protect personnel when removing Cryules from cryogenic gases.

Sterile External threads2 mL sizeSupplied with colored coded capsConical bottom

These polypropylene vials are freestanding on the bench and lock into the rack (Cat. No. 985810) for one-handed cap removal. The vertical inner wall and conical bottom facilitate filling and maximum sample retrieval. The white marking area and color coded caps allow for lot and sample identification. Vials are packaged 50 per bag,10 bags per case.

Cat. No. Cap Color Size (mL) Dia x H (mm) Qty/Pack Qty/Case

985734 White 2 12 x 46 50 500985735 Red 2 12 x 46 50 500985736 Pink 2 12 x 46 50 500985737 Yellow 2 12 x 46 50 500985738 Green 2 12 x 46 50 500985739 Blue 2 12 x 46 50 500

•••••

Sterile Internal threadsSilicone ring sealing system1.2, 2 and 4 mL size

These freestanding polypropylene vials are internally threaded. These vials and caps feature a streamlined design with a silicone ring sealing system. Color coded cap inserts can be used for lot identification. For cap inserts, see catalog number series 242582. Vials are packaged 50 per bag,10 bags per case.

Cat. No. Size (mL) Dia x H (mm) Qty/Pack Qty/Case

985745 1.2 12 x 42 50 500985746 2 12 x 49 50 500985747 4 12 x 71 50 500

••••

Cryule Vial with Colored Caps Vial Rack for one-hand cap removal, 985810

DMSO (dimethyl sulfoxide)

Ethylene glycol

Glycerol

Propylene glycol

Sucrose

Trehalose

•

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•

•

•

•

Liquid nitrogen (LN2): liquid density at the triple point is

0.807 g/mL, is inert, colorless, odorless, non-corrosive, nonflammable, and extremely cold.

Liquid nitrogen boils at -196°C, and is a cryogenic fluid which is potentially capable of causing rapid frostbite on contact with living tissue.

Cryopreservation is a process where cells or whole tissues are preserved by cooling to low sub-zero temperatures, such as (typically) 77 K or −196°C (the boiling point of liquid nitrogen).

•

•

•

When cryoprotectant solutions are not used, the cells being preserved are often damaged due to freezing during the approach to low temperatures or warming to room temperature.

A cryoprotectant is a substance that is used to protect biological tissue from freezing damage (damage due to ice formation).

Cryoprotectants act like antifreeze: they lower the freezing temperature. They also increase the viscosity. Instead of crystallizing, the syrupy solution turns into an amorphous ice.

•

•

•

Freestanding Cryule Vial Cap Inserts

See Cap Inserts on page 142.For Cryogenic Ampules see the Ampule Section on page 1.

Non-sterileExternal threadRound bottom2 mL sizeScrew cap includedAutoclavable

These externally threaded polypropylene vials come with white polypropylene caps. The cap attaches to the outside of the vial, which means you get maximum vial capacity while the lip, as well as your hands, are protected from contamination. The vial and cap can be autoclaved. Vials are available with or without the white marking area for sample identification. Vials and caps are packed separately in polyethylene bags. Color coded caps can be purchased separately. See catalog numbers 242564 and 242566.


985721 2 mL Cryule Vial with Marking Area 12 x 44 1000985726 2 mL Cryule Vial without Marking Area 12 x 44 1000

Sterile External threadsRound bottom1 and 2 mL sizeWhite screw cap

These externally threaded vials are more compact than most vials, and safely store the total labeled volume. Vials are manufactured from medical grade polypropylene, and they come sterilized with caps attached. They are packaged 50 per bag, 10 bags per case. Color coded caps can be purchased separately. See catalog numbers 242564 and 242566.


985730 1 12 x 24 50 500 985731 2 12 x 44 50 500

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•••••

Sterile Internal threadsRound bottom2, 4 and 5 mL sizeColor code cap inserts available separately

These uniquely designed vials are more compact than most vials, and safely store the total labeled volume. Internally threaded vials have silicone ring seals, which ensure secure cap sealing. The vials come sterilized with caps attached. 50 per bag, 10 bags per case. Color coded cap inserts can be purchased separately for lot coding. See catalog number series 242582.


985742 2 12 x 48 50 500985743 4 12 x 70 50 500985744 5 12 x 90 50 500

See cap inserts on page 142.

SterileColor codedMade from polypropyleneAutoclavable

These 10 mm caps are designed for easy identification of Cryule® Vials. They fit Cryule Vials 985721, 985726, 985730, and 985731. The caps are sterilized and packed in polybags.


242564 Blue 100242566 Green 100

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••••

SterileSterile

Non

SterileNon

Sterile

Internal

ThreadInterna

l

Thread

Cap Inserts

Color Code your Cryule Vials with Wheaton Colored Caps (Cat. No. 242564, 242566, and 242572)

141


Cryogenic

If you need to barcode your cryogenic vial, Wheaton has the SciLution®. See pages 133-134 for more details.

142Culture/Dilution/EPA


Color coded for sample and lot identificationPolypropyleneFit vials catalog numbers 985742, 985743, 985744, 985745, 985746 & 985747

For use with internally threaded Cryule® Vial caps. Use these colored inserts for sample and lot identification. They are compatible with 985742 and 985745 series internally threaded Wheaton Cryule® Vials.


242582 Red 500242584 Blue 500242586 Green 500242588 Yellow 500242590 White 500242592 Assorted (100 of each color) 500

For 1 and 2 mL Cryule® Vials Holds 81 Vials

Engineered for use with liquid nitrogen, these convenient storage units can also be used in a freezer or at room temperature. These Freezer Boxes are made of chipboard and spray-coated with polyethylene. Accommodates 81 of either 1 or 2mL Cryule® vials.

Cat. No Fits Dimensions (L x W x H) Qty/Case

651490 1 mL Cryule Vials 5-1/4 x 5-1/4 x 1-1/4 in (13.3 x 13.3 x 3.1 cm) 15 651492 2 mL Cryule Vials 5-1/4 x 5-1/4 x 1-3/4 in (13.3 x 13.3 x 4.4 cm) 15

For use with freestanding Cryule Vials

Allows for one-handed cap removalHolds 50 vialsPolypropyleneAutoclavable

This unique rack securely locks externally threaded freestanding Cryule® Vials in place for one-handed cap removal. Non-skid feet offer additional stability for bench work. This 50-position rack will also work with other freestanding cryogenic vials. Rack is made of durable, autoclavable polypropylene. This rack allows easy sample identification with an alphanumeric index on top of each rack. Molded corner posts provide stability when stacking. Rack can be autoclaved at 121°C for 20 minutes. Dimensions Cat. No. Well ID Description No. of Wells (L x W x H) Qty/Case

985810 12.5 mm 50-Position Rack 5 deep x 10 wide 7-5/8 x 4 x 7/8 in (19 x 10 x 2.2 cm) 5

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4, 8 and 12 mL sizeWheaton 33 low extractable borosilicate glassDeep-skirted black phenolic screw cap

with 14B rubber linerAutoclavable

These culture vials feature deep-skirted screw caps to allow safer, more dependable handling when working with infectious materials. Pre-attached solid-top black phenolic screw cap with 14B rubber liner can be autoclaved. Vials are packaged in cor-rugated trays with partitions. Vials are manufactured from Wheaton 33 low extractable borosilicate glass that conforms to ASTM Type I, Class A and USP Type I requirements. Use M-T Vial File (Cat. No. 228780) for storing 4 mL vials.

Cat. No. Size (mL) *Dia x H (mm) Cap Size Qty/Case

225142 4 15 x 51 13-415 144225144 8 17 x 70 15-415 144225145 12 19 x 72 15-415 144

* Measurement taken with cap attached.

4 mL sizeMarked with blue line at 3 mL levelPre-attached solid-top black phenolic screw cap with

14B rubber linerAutoclavable

Specially suited for research studies, this vial is marked with a blue line at the 3 mL level. It can be sterilized and stored at low temperatures. Vials are packaged in corrugated trays with partitions. They are manufactured from Wheaton 33 low

extractable borosilicate glass that conforms to ASTM Type I, Class A and USP Type I requirements to protect against changes in pH and to maintain the purity of the contents. Use M-T Vial File (Cat. No. 228780) for storing vials.


225002 4 15 x 48 13-425 144


Low extractable borosilicate glassVials packaged in convenient trays

for ease of useCaps attached to vials

These vials are ideal for use in water sampling according to EPA 40 CFR 136, “Guidelines for Establishing Test Procedures for the Analysis of

Pollutants.” The clear vials are manufactured from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements, and the amber vials are manufactured from Wheaton 320 amber glass that conforms to USP Type I requirements to protect light-sensitive samples and to maintain purity of its contents. All vials are packaged in convenient trays which facilitates handling.

Cat. No. Size (mL) Size (dr) *Dia x H (mm) Cap Size Qty/Case

Clear Vial with Open Top Black Phenolic Teflon® Faced Silicone Lined Cap 225310 40 10 28 x 98 24-400 72

Amber Vial with Open Top White Polypropylene Teflon® Faced Silicone Lined Cap225315 40 10 28 x 98 24-400 72

*Measurement taken with cap attached.


W240518 Black Phenolic Open Top Screw Cap w/o septa 24-400 200W240598 10 mils Teflon®/90 mils Silicone Septa for 24-400 screw cap — 100W224600 White Glass-Filled Open Top Polypropylene Cap with Bonded 5 mils Teflon®/120 mils Silicone Septa 24-400 200

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143


High Recovery

These autoclavable caps are designed to ensure the ultimate in product safety. The liners are made of a nontoxic white styrene-butadiene rubber and are faced with Teflon which offers excellent chemical resistance. They are available in convenient lab pack quantities as well as larger case packs.


W240820 13-425 250 250W240821 13-425 250 1000W240822 15-425 250 250W240823 15-425 250 1000W240824 22-400 100 100W240825 22-400 100 500240418 24-400 100 100W240827 24-400 100 500

These white autoclavable caps have a bonded Teflon faced silicone liner making them ideal for storage and containment of volatile solvents, sensitive compounds, and corrosive chemicals. The liners consist of 0.055" (1.4 mm) of silicone and 0.005" (0.14 mm) of Teflon. They are available in convenient lab pack quantities as well as larger case packs.


W240830 13-425 250 250W240831 13-425 250 1000W240832 15-425 250 250W240833 15-425 250 1000W240834 22-400 100 100W240835 22-400 100 500W240836 24-400 100 100W240837 24-400 100 500

These autoclavable open top caps have a bonded Teflon faced silicone liner mak-ing them ideal for storage and retrieval of volatile solvents, sensitive compounds and corrosive chemicals. The liners consist of 0.055" (1.4 mm) of silicone and 0.005" (0.14 mm) of Teflon. They are available in convenient lab pack quantities as well as larger case packs.

Cat. No. Cap Size Color Qty/Pack Qty/Case

W242710 13-425 Black 250 250W240840 13-425 White 250 250W240841 13-425 White 250 1000W240842 15-425 White 250 250W240843 15-425 White 250 1000W240844 22-400 White 100 100W240845 22-400 White 100 500W240846 24-400 White 100 100W240847 24-400 White 100 500

Black Phenolic Screw Cap Polypropylene Screw Cap Polypropylene, Open Top, Screw Cap

NewNewNewNew

Conical well ensures maximum sample recoveryQuality engineered for automated sample handling systemsMade from low extractable borosilicate glassExcellent for Compound Storage

The new Wheaton E-Z Ex-Traction Vials combine the best attributes of high recovery vials with a unique bottom to provide the optimum benefits to users of automated or manual handling and storage systems in compound or biological sample applications. Precision manufactured from low extractable borosilicate glass, E-Z Ex-Traction Vials conform to ASTM Type I, Class A and USP Type I requirements. E-Z Ex-Traction Vials are supplied in corrugated trays with SBS (solid bleach sulfate) partitions which main-tain clean vial conditions during shipping and storing prior to use. Custom packaging options are also available to meet customer specifications.Cat. No. Size (mL) Dia x H (mm) Cap Size Qty/Pack Qty/Case

W224601 2 15 x 32 13-425 250 250W224603 2 15 x 32 13-425 250 1000W224613 4 15 x 45 13-425 250 250W224617 4 15 x 45 13-425 250 1000W224618 8 16.75 x 60 15-425 250 250W224619 8 16.75 x 60 15-425 250 1000W224630** 10 19 x 70 22-400 100 100W224632** 10 19 x 70 22-400 100 500W224634 20 26.5 x 67 24-400 100 100W224636 20 26.5 x 67 24-400 100 500

Wheaton offers E-Z Ex-Traction Vials in five standard sizes and configurations. If your automated system is designed to handle vials with different dimensions or if your process requires vials to contain specific volumes, we can meet your requirements.

To provide your specifications and to receive a quote, contact your local Wheaton Sales Representative at 800-225-1437 or your local International Distributor.*The bottom design of these vials creates a large, smooth and flat surface that offers greater stability and gives the user the option of applying larger 2D bar codes.

**Shoulderless vial

••••

NewNew

Engineered for Automated Sample Handling Systems

When you need to apply a treatment to your container Wheaton has the SciLutions®.

Bar Coding Critical Cleaning Surface Treatment Taring Custom Containers

For more information see pages 133-134 or contact your local Wheaton Sales Representative.

Patented Design

144High Recovery


New fire glazed bottom protects against glass particulatesNew low particulate packaging protects against contamination during

transportation and storageConical interior bottom provides downward drainageMade from clear and amber borosilicate glassAvailable with solid and open top screw cap or serum finish

The new NextGen V Vials were created to provide users with all of the benefits of our original V Vials but with some additional enhancements. The new fire glazed bottom protects against glass particulates while the new low particulate packaging protects against contamination during transportation and storage. These exceptionally sturdy vials have a conical interior bottom that allows for maximum retrieval of content by syringe or micropipette. V Vials can be used for small-scale reactions, centrifugation storage and shipping. Clear V Vials are manufactured from Wheaton 33 low extractable borosilicate glass that conforms to ASTM Type I, Class A and USP Type I requirements to safeguard against a change in product pH caused by alkali, which can be extracted by ordinary glass. Amber V Vials are manufactured from Wheaton 320 low extractable borosilicate glass that conforms to USP Type I requirements. V Vials are used for small-scale reactions with Wheaton Micro Kit catalog number 774600. They are also used in the biochemical, pharmaceutical and radiochemical industries.

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Clear glass vialSolid top black phenolic capTeflon® faced 14B rubber linerScrew cap attached

Cat. No Size (mL) Dia x H (mm) (cap on) Cap Size Qty/Case

W986273NG** 0.3 13 x 35 13-425 12W986274NG** 1.0 13 x 44 13-425 12W986276NG** 2.0 17 x 61 15-415 12W986272NG** 2.0 20 x 44 20-400 12W986277NG** 3.0 20 x 50 20-400 12W986279NG** 5.0 20 x 65 20-400 12

Clear Vial with Solid Top Black Phenolic Teflon® Faced 14B Rubber Lined Caps AttachedW986253NG 0.3 13 x 35 13-425 12W986254NG 1.0 13 x 44 13-425 12 W986256NG 2.0 17 x 61 15-415 12W986261NG 2.0 20 x 44 20-400 12W986257NG 3.0 20 x 50 20-400 12W986259NG 5.0 20 x 65 20-400 12W986260NG 10.0 24 x 72 24-400 6

Clear Vial with Open Top Black Phenolic Teflon® Faced Silicone Lined Caps AttachedW986281NG 0.1 12 x 35 8-425 12W986282NG 0.3 12 x 35 8-425 12W986283NG 0.3 13 x 35 13-425 12W986284NG 1.0 13 x 44 13-425 12 W986288NG 2.0 20 x 44 20-400 12W986287NG 3.0 20 x 50 20-400 12W986289NG 5.0 20 x 65 20-400 12W986290NG 10.0 24 x 72 24-400 6

*Measurement taken with cap attached. ** Graduated

Clear graduated vialOpen top black phenolic capTeflon® faced silicone linerScrew cap attached


W986293NG 0.3 13 x 35 13-425 12W986294NG 1.0 13 x 44 13-425 12W986298NG 2.0 20 x 44 20-400 12W986297NG 3.0 20 x 50 20-400 12W986299NG 5.0 20 x 65 20-400 12

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••••

Anaerobic reactions

Catalytic hydrogenation

Centrifugation

Concentration

Derivatization

Extraction

Homogenization

Hybridization

Micro mixing

Packaging

Radiosynthesis

NextGen™ V Vial® Packaging

Non-graduated glass vialCrimp top finishClosure sold separately*

Cat. No Size (mL) Mouth ID x OD (mm) Dia x H (mm) Qty/Case

W986211NG 0.1 5 x 11 12 x 32 12W986212NG 0.3 5 x 11 12 x 32 12W986213NG 0.3 7 x 13 13 x 32 12W986214NG 1.0 7 x 13 13 x 41 12W986216NG 2.0 13 x 20 20 x 40 12W986217NG 3.0 13 x 20 20 x 46 12W986219NG 5.0 13 x 20 20 x 61 12

*Closure not included. Please see the Cap and Closure section on pages 32-35 for a complete listing of aluminum seals and rubber stoppers.

Note: When choosing aluminum seal and stopper use the mouth OD dimension of the vial.

Non-graduated glass vialSolid top black phenolic capTeflon® faced 14B rubber linerScrew cap attached



•••

••••

Non-graduated glass vialOpen top black phenolic capTeflon® faced silicone linerScrew cap attached



Non-graduated glass vialScrew thread finishClosure sold separately*

Cat. No Size (mL) Dia x H (mm) Cap Size Qty/Case


*Closure not included. For a complete listing of screw caps see page 37.

Used to stir sample within the V Vial®

Magnetic bar in V-shape

Use the Wheaton Spin Vane with a magnetic stirrer when you need to stir your sample in the V Vial. The Teflon® Spin Vane has a cross-mounted, molded-in magnetic bar in a V-shape designed specifically for use in V Vials®.


903061 Fits 0.3 and 1 mL V Vials 6903063 Fits 2, 3, 5 and 10 mL V Vials 6

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Replacement caps for V Vials are located on page 37.

145


High Recovery

146Liquid Scintillation


Wheaton has been supplying scintillation vials to the scientific community for decades. Researchers utilize the vials, not only for scintillation counting instrumentation, but also as sample vials and for transferring samples from one lab to another. Quality, a Wheaton hallmark, allows you to depend on us for providing superior products for research requirements. For your scintillation or sample vial requirements select from glass, HDPE or PET vials.

Glass Vials are manufactured from Wheaton 180 low potassium borosilicate glass that conforms to ASTM Type I, Class A and USP Type I requirements. Critical manufacturing tolerances ensure reliable, consistent results for scintillation counting. Vials load properly into counting machines without jamming or break-ing. Background counts are consistent and low;

ultraviolet transmission is high. White caps fit tightly to avoid solubilizer fumes in the laboratory and have tops suitable for writing.

HDPE Vials are made from high density polyethylene which features lightweight walls for increased counting efficiency. These premium vials are engineered and produced to precise tolerances to avoid jamming. Conventional screw thread caps ensure the most effective seal against loss of contents and fume contamination in your laboratory.

PET Vials manufactured from crystal clear polyethylene terephthalate offer significant advantages over glass counting vials, including a low background count, having the clarity of glass with the safety of plastic,

and they are economical.

20 mL size Made from Wheaton 180 low potassium borosilicate glass Background counts are consistent and low; ultraviolet transmission is highAvailable with cap attached, caps packed separately or without capDimensions (OD x H): 28 mm x 61 mm (with cap attached)

•••••

Cat. No. Cap Size Cap Material Liner Material Qty/Case

Vials without Screw Caps986532 22-400 — — 500

Vials with Screw Caps Attached986540 22-400 Polypropylene Foamed Polyethylene 500986541 22-400 Polypropylene Metal Foil 500986542 22-400 Urea Metal Foil 500986546 22-400 Urea Poly Seal Cone 500986548 22-400 Urea Polyethylene Disc 500986560 24-400 Polypropylene Foamed Polyethylene 500986561 24-400 Polypropylene Metal Foil 500986562 24-400 Urea Metal Foil 500986568 24-400 Urea Polyethylene Disc 500

Vials with Screw Caps Packed Separately986580 22-400 Polypropylene Foamed Polyethylene 500986581 22-400 Polypropylene Metal Foil 500986582 22-400 Urea Metal Foil 500986586 22-400 Urea Poly Seal Cone 500986590 24-400 Polypropylene Foamed Polyethylene 500986591 24-400 Polypropylene Metal Foil 500

147


Liquid Scintillation

20 mL size Made from high density polyethylene Available with screw cap attached or with caps packed separatelyDimensions (OD x H): 27 mm x 61 mm (with cap attached)Shrink-wrapped in plastic

These 20 mL vials are manufactured from high density polyethylene. Vials with caps attached are packaged in individual cells in trays of 100. Vials with screw caps packed separately are packaged in polyethylene bags of 100 and placed in a separate tray. Cases of 1000 vials are packed in a single plastic bag with caps in a separate bag. Trays of 100 vials are shrink-wrapped in plastic for protection against contamination.


Vials with Screw Caps Attached986700 22-400 Polypropylene Foamed Polyethylene 500986701 22-400 Polypropylene Metal Foil 500986702 22-400 Urea Metal Foil 500986704 22-400 Polyethylene Linerless 500986706 22-400 Urea Poly-Cone 500

Vials with Screw Caps Packed Separately986710 22-400 Polypropylene Foamed Polyethylene 500986720 22-400 Polypropylene Foamed Polyethylene 1000986711 22-400 Polypropylene Metal Foil 500986721 22-400 Polypropylene Metal Foil 1000986712 22-400 Urea Metal Foil 500986722 22-400 Urea Metal Foil 1000986714 22-400 Polyethylene Linerless 500986724 22-400 Polyethylene Linerless 1000986716 22-400 Urea Poly Seal Cone 500986726 22-400 Urea Poly Seal Cone 1000

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4 mL size Made from polypropylenePolypropylene press-fit cap packed separately Dimensions (OD x H): 13 mm x 57 mmAutoclavable

These vials are designed for beta and gamma counting, but they can also be used for RIA, liquid scintillation counting, sample storage, sample counting and chromatography. Omni-Vials have a smooth interior allowing easy transfer and access with a pipettor. Polyolefins used in these vials can be safely incinerated without the formation of hazardous gases. Vials and caps are packed separately in two polybags that contain 500 each. A polybag liner is also included for easy disposal of the vials. Vial can be autoclaved for 15 minutes at 121°C at 15 psi.

Cat. No. Description Cap Material Liner Material Qty/Case

225402 4 mL Omni-Vial Polypropylene Linerless 1000

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20 mL size Made from polyethylene terephthalate (PET)Clarity of glass with safety of plastic Low permeability to solvents and minimal background countAvailable with screw cap attached or with caps packed separately Dimensions (OD x H): 27 mm x 61 mm (with cap attached)

For optimal performance in the laboratory, PET plastic vials provide the clarity of glass and have the safety of using plastic. Vials can be safely incinerated; no harmful gas is generated; only CO

2 and H

2O are created. Lightweight, and

virtually unbreakable PET vials offer low permeability to solvents and minimal background counts. Trays of 100 vials are shrink-wrapped in plastic for protection against contamination.


Vials with Screw Caps Attached986730 22-400 Polypropylene Foamed Polyethylene 500986731 22-400 Polypropylene Metal Foil 500986732 22-400 Urea Metal Foil 500986734 22-400 Polyethylene Linerless 500986736 22-400 Urea Poly Seal Cone 500

Vials with Screw Caps Packed Separately986750 22-400 Polypropylene Foamed Polyethylene 500986740 22-400 Polypropylene Foamed Polyethylene 1000986751 22-400 Polypropylene Metal Foil 500986741 22-400 Polypropylene Metal Foil 1000986752 22-400 Urea Metal Foil 500986742 22-400 Urea Metal Foil 1000986754 22-400 Polyethylene Linerless 500986744 22-400 Polyethylene Linerless 1000986756 22-400 Urea Poly Seal Cone 500986746 22-400 Urea Poly Seal Cone 1000

••••••

148Liquid Scintillation


6 mL size Made from high density polyethyleneAvailable with quarter-turn cap or press fit capDimensions (OD x H): 17 mm x 57 mm (with cap attached)

These 6 mL vials are manufactured from high density polyethylene and have a quarter-turn cap. The hanging cap allows vial suspension and easy access when used with Wheaton glass and PET, 20 mL liquid scintillation vials with 22 mm neck size.

Cat. No. Description Cap Material Qty/Case

225414 6 mL Vial with Press Fit Cap Polypropylene 1000225415 6 mL Vial with Hanging Cap Polypropylene 1000

Cat. No. Cap Size (mm) Cap Material Cap Liner Material Qty/Case

240804 22-400 Polypropylene Metal Foil 1000240805 24-400 Polypropylene Metal Foil 1000240817 22-400 Urea Polyethylene Disc 1000240818 24-400 Urea Polyethylene Disc 1000240917 22-400 Urea Poly Seal Cone 1000241009 15-425 Urea Metal Foil 1000241017 22-400 Urea Metal Foil 1000241018 24-400 Urea Metal Foil 1000241317 22-400 Polyethylene Linerless 1000

2 mL sizeSmall size reduces sample to surface contactMade from Wheaton 33 low extractable borosilicate glassAttached polypropylene screw cap with metal foil liner

These vials are packaged in corrugated trays with partitions. Metal foil liner has excellent barrier properties for hydrocarbons. Use M-T Vial File Cat. No. 228778 for storing vials.


225081 2 12 x 38 8-425 288


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••••

6 mL size Made from Wheaton 180 low potassium borosilicate glass Background counts are consistent and low; ultraviolet transmission is highScrew caps packed separately Dimensions (OD x H): 17 mm x 58 mm (with cap attached)

These 6 mL disposable glass sample vials are economical when used as liquid scintillation vials. They are manufactured from Wheaton 180 low potassium borosilicate glass that conforms to ASTM Type I, Class A and USP Type I requirements. Vials are packed in individual cells in trays of 200. Vials in trays are shrink-wrapped in plastic for protection against contamination. Catalog number 986491 is specially designed for those instruments requiring a cap smaller in diameter than the vial to prevent jamming.


986491 13-425 Urea Metal Foil 1000986492 15-425 Urea Metal Foil 1000

6 mL size Made from high density polyethyleneScrew caps packed separately Dimensions (OD x H): 17 mm x 57 mm (with cap attached)

These 6 mL vials are manufactured from high density polyethylene and are economical when used as liquid scintillation vials. Vials and caps are bulk packed in two separate plastic bags.


986644 18 Polypropylene Linerless 1000986645 18 Polypropylene Linerless 2000

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••••



149


Racks

Size of rack depends on vial ODEasy to clean and autoclavableAlphanumeric indexing

Wheaton vial racks come in a variety of sizes. The racks are manufactured from polypropylene and can be easily cleaned in an automatic washer or autoclaved. The racks are sturdy and can be stacked, even when the vials are in place. Each well has an opening in the bottom to facilitate drainage. Autoclavable at 121°C for 20 minutes.

•••

Clear plastic hinged case for use with 2 and 4 mL vialsConvenient for storing and transporting vialsPartitions and foam inserts protect vials Includes alphanumerical indexing cardExtra partitions and index cards can be purchased separately

•••••

The M-T Vial File® provides a compact, easily accessible means of sample storage. The vials are stored in individual partitions within a clear polystyrene case. Foam inserts and partitions provide extra protection for the vials. The alphanumerical indexing card allows for identification of sample vials. M-T Vial File comes with polystyrene case, partitions, foam inserts and indexing card. Partitions and index cards can also be purchased separately.

Cat. No. Description Stores Qty/Case

Complete M-T Vial File without Vials228778 M-T Vial File 60 – 2 mL vials 6228780 M-T Vial File 40 – 4 mL vials 6

Index Cards228783 For use with 228778 60 – 2 mL vials 35228785 For use with 228780 40 – 4 mL vials 35

PartitionsW228781 For use with 228778 60 – 2 mL vials 6W228782 For use with 228780 40 – 4 mL vials 6

Cat. No. Well ID (mm) Description No. of Wells Dimensions (L x W x H) Qty / Case

Polypropylene Rack985750 9.5 96-Position Rack 8 deep x 12 wide 7 1/4 x 5 x 7/8 in 18.5 x 12.5 x 2.2 cm 5985800 12.5 50-Position Rack 5 deep x 10 wide 7 5/8 x 4 x 7/8 in 19 x 10 x 2.2 cm 5985810* 12.5* 50-Position Rack 5 deep x 10 wide 7 5/8 x 4 x 7/8 in 19 x 10 x 2.2 cm 5868804 15.5 48-Position Rack 4 deep x 12 wide 10 5/8 x 3 3/4 x 1-1/8 in 26.6 x 9.4 x 2.8 cm 5868810 17.1 90-Position Rack 6 deep x 15 wide 13 x 6 3/4 x 1-1/4 in 33 x 17 x 3 cm 5868805 23.1 36-Position Rack 3 deep x 12 wide 12 3/4 x 3 5/8 x 1-1/8 in 32.2 x 9.1 x 2.8 cm 5868806 28.1 50-Position Rack 5 deep x 10 wide 13 x 6 3/4 x 1-1/4 in 33 x 17 x 3 cm 5868808 30.0 50-Position Rack 5 deep x 10 wide 13 1/4 x 6 3/4 x 1-1/4 in 33.7 x 17 x 3 cm 5

Disposable Polystyrene Rack 868608 23 50-Position Rack 5 deep x 10 wide 10 1/2 x 5 1/2 x 1 1/8 in 26.7 x 14 x 2.9 cm 5868615 43 48-Position Rack 4 deep x 12 wide 14 3/4 x 11 1/8 x 1 3/4 in 37.5 x 28.3 x 4.4 cm 8 868617 52 35-Position Rack 5 deep x 7 wide 15 3/4 x 11 1/2 x 2 1/2 in 40 x 29.2 x 6.4 cm 8

*This rack is specially designed for use with Freestanding Cryule® Vials. The vials lock in the rack for one-handed cap removal.

30 mm Well ID 50-Position 868808

9.5 mm Well ID 96-Position 985750


12.5 mm Well ID 50-Position 985810*


17.1 Well ID 90-Position 868810

23.1mm Well ID 36-Position 868805


PolystyreneThree sizes availableCatalog numbers -

868608, 868615 & 868617

•••

Selecting The Proper Rack: When selecting the proper rack, the vial OD can not exceed the well ID dimension.

150Sample


Vials with caps attached packaged in partitioned traysClear and amber vials made from borosilicate glassShorty Vials® feature a low profile for greater stabilityChoice of screw cap liners for aqueous or organic samples

Sample Vials in Lab File are packaged in corrugated trays with partitions. Clear vials are manufactured from Wheaton 33 low extractable borosilicate glass that conforms to ASTM Type I, Class A and USP Type I requirements, and amber vials are manufactured from Wheaton 320 glass that conforms to USP Type I requirements to protect light-sensitive samples. Shorty Vials

feature a low profile for greater stability and less dead volume above the sample, mak-ing it ideal for compound storage. Catalog numbers 225012, 225014 have a white marking area for sample identification. Black phenolic screw caps are attached to vials. It is suggested to use 14B rubber lined

caps for aqueous samples, and Teflon® faced 14B rubber lined caps for organic samples.

Vials and caps are autoclavable. Replacement caps can be purchased separately. See pages 37

and 152 for screw cap ordering information.

••••

(with caps attached)Cat. No. Size (mL) Size (dr) Dia x H (mm) (cap on) Cap Size Qty/Case

Clear Vials with Solid Top Black Phenolic Teflon® Faced 14B Rubber Lined CapsW224581 2* 1/2 12 x 38 8-425 288W224582 4** 1 15 x 48 13-425 144W224584 8 2 17 x 63 15-425 144W224585 12 3 19 x 68 15-425 144W224586 16 4 21 x 73 18-400 144W224588 24 6 23 x 88 20-400 144W224589 20 5 28 x 60 24-400 72W224590 25 6 1/4 28 x 73 24-400 72W224591 40 10 28 x 98 24-400 72

Amber Vials with Solid Top Black Phenolic Teflon® Faced 14B Rubber Lined CapsW224681 2* 1/2 12 x 38 8-425 288W224682 4** 1 15 x 48 13-425 144W224684 8 2 17 x 63 15-425 144W224604 20 5 28 x 57 24-400 72W224605 40 10 28 x 95 24-400 72

Clear Shorty Vials® with Solid Top Black Phenolic Teflon® Faced 14B Rubber Lined CapsW224606 2* 1/2 15 x 31 13-425 200W224607 4** 1 17 x 41 15-425 200W224608 6 1 1/2 19 x 43 15-425 200

*Fits the M-T Vial File, 228778.

**Fits the M-T Vial File, 228780.

(with caps attached) Cat. No. Size (mL) Size (dr) Dia x H (mm) (cap on) Cap Size Qty/Case

Clear Vials with Open Top White Polypropylene Teflon® Faced Silicone Lined Caps (Septa of 5 mils of Teflon® facing 120 mils of silicone is bonded to cap.)

W224609 20 5 28 x 60 24-400 72W224610 25 6.25 28 x 73 24-400 72W224611 40 10 28 x 98 24-400 72

Amber Vials with Open Top White Polypropylene Teflon® Faced Silicone Lined Caps

(Septa of 5 mils of Teflon® facing 120 mils of silicone is bonded to cap.)

W224612 20 5 28 x 60 24-400 72W224614 40 10 28 x 98 24-400 72

Clear Vial Amber Vial Vial with Writing Patch Shorty Vial®

(with caps attached)Cat. No. Size (mL) Size (dr) Dia x H (mm) (cap on) Cap Size Qty/Case

Clear Vials with Solid Top Black Phenolic 14B Rubber Lined Caps 224881 2* 1/2 12 x 38 8-425 288224882 4** 1 15 x 48 13-425 144224884 8 2 17 x 63 15-425 144224885 12 3 19 x 68 15-425 144224886 16 4 21 x 73 18-400 144224888 24 6 23 x 88 20-400 144225288 20 5 28 x 60 24-400 72225289 25 6.25 28 x 73 24-400 72225290 40 10 28 x 98 24-400 72

Amber Vials with Solid Top Black Phenolic 14B Rubber Lined Caps224981 2* 1/2 12 x 38 8-425 288224982 4** 1 15 x 48 13-425 144224984 8 2 17 x 63 15-425 144W224820 20 5 28 x 60 24-400 72W224840 40 10 28 x 98 24-400 72

Clear Vials with White Marking Area and Solid Top Black Phenolic 14B Rubber Lined Caps225012 4** 1 15 x 48 13-425 144225014 8 2 17 x 63 15-425 144

Clear Shorty Vials® with Solid Top Black Phenolic 14B Rubber Lined CapsW225291 2* 1/2 15 x 31 13-425 200W225292 4** 1 17 x 41 15-425 200W225293 6 1 1/2 19 x 43 15-425 200

*Fits the M-T Vial File, 228778.

**Fits the M-T Vial File, 228780.

151


Sample

(With Screw Cap)EConomically pricedVials and screw caps packaged separatelyClear and amber vials made from borosilicate glassChoice of screw cap liners for aqueous or organic samples

These economically priced sample vials are conveniently packed in two shrink-wrapped packs of 100 vials. This packaging reduces contamination from corrugated material found in traditional packaging. The clear vials are manufactured from Wheaton 33 low extractable borosilicate glass that conforms to ASTM Type I, Class A and USP Type I requirements, and the amber vials are manufactured from Wheaton 320 amber glass that conforms to USP Type I requirements to protect light-sensitive samples. Black phenolic screw caps are packaged separately and inserted in the box with the vials. It is suggested to use 14B rubber lined caps for aqueous samples and Teflon® faced 14B rubber lined caps for organic samples. Replacement caps can be purchased separately.

••••

(without caps)Clear and amber vials made from borosilicate glassShorty Vials® feature a low profile for greater stabilityScrew caps available separately

Cat. No. Size (mL) Size (dr) Dia x H (mm) Cap Size Qty/Case

Clear Vials without Caps 224801 2 1/2 12 x 35 8-425 200224802 4 1 15 x 45 13-425 200224804 8 2 17 x 60 15-425 200224805 12 3 19 x 65 15-425 200224806 16 4 21 x 70 18-400 200224808 24 6 23 x 85 20-400 200224831 20 5 28 x 57 24-400 200224832 25 6-1/4 28 x 70 24-400 200224833 40 10 28 x 95 24-400 200

•••

Lab File™ without Caps Clear Vials Amber Vials Shorty Vial®

Cat. No. Size (mL) Size (dr) Dia x H (mm) Cap Size Qty/Case

Amber Vials without Caps224811 2 1/2 12 x 35 8-425 200224812 4 1 15 x 45 13-425 200224814 8 2 17 x 60 15-425 200W224815 20 5 28 x 57 24-400 200224836 40 10 28 x 95 24-400 200

Clear Shorty Vials® without Caps224821 2 1/2 15 x 28 13-425 200224822 4 1 17 x 38 15-425 200224823 6 1-1/2 19 x 40 15-425 200


Clear Vial with Solid Top Black Phenolic 14B Rubber Lined Caps224720 1.8 3/8 12 x 35 8-425 200224721 2 1/2 12 x 38 8-425 200224722 4 1 15 x 48 13-425 200224724 8 2 17 x 63 15-425 200224725 12 3 19 x 68 15-425 200224726 16 4 21 x 73 18-400 200224728 24 6 23 x 88 20-400 200

Amber Vials with Solid Top Black Phenolic 14B Lined Caps224730 1.8 3/8 12 x 35 8-425 200224731 2 1/2 12 x 38 8-425 200224732 4 1 15 x 48 13-425 200224734 8 2 17 x 63 15-425 200


Clear Vials with Solid Top Black Phenolic Teflon® Faced 14B Rubber Lined Caps224740 1.8 3/8 12 x 35 8-425 200224741 2 1/2 12 x 38 8-425 200224742 4 1 15 x 48 13-425 200224744 8 2 17 x 63 15-425 200224745 12 3 19 x 68 15-425 200224746 16 4 21 x 73 18-400 200224748 24 6 23 x 88 20-400 200

Amber Vials with Solid Top Black Phenolic Teflon® Faced 14B Rubber Lined Caps224750 1.8 3/8 12 x 35 8-425 200224751 2 1/2 12 x 38 8-425 200224752 4 1 15 x 48 13-425 200224754 8 2 17 x 63 15-425 200

* Measurement is taken with cap attached.

152Sample


(Without Screw Cap)Cat. No. Size (mL) Size (dr) Dia x H (mm) Cap Size Qty/Case

Clear Vials without Screw Caps 224700 1.8 3/8 12 x 32 8-425 200224701 2 1/2 12 x 35 8-425 200224702 4 1 15 x 45 13-425 200224704 8 2 17 x 60 15-425 200224705 12 3 19 x 65 15-425 200224706 16 4 21 x 70 18-400 200224708 24 6 23 x 85 20-400 200W224809 20 5 28 x 57 24-400 200W224810 40 10 28 x 95 24-400 200W226060 60 15 30 x 125 24-400 143

Amber Vials without Screw Caps224710 1.8 3/8 12 x 32 8-425 200224711 2 1/2 12 x 35 8-425 200224712 4 1 15 x 45 13-425 200224714 8 2 17 x 60 15-425 200

Solid-top black phenolic screw capReplacement caps for Lab File vialsChoice of cap linerAutoclavable


With 14B White Rubber Liner 240206 8-425 1000240208 13-425 200240209 15-425 200240215 18-400 500240216 20-400 500240218 24-400 500

With Teflon® Faced Rubber LinerW240406 8-425 200240408 13-425 200240409 15-425 200240414 18-415 200240415 18-400 200240416 20-400 100240418 24-400 100W242711 24-400 200

••••

Clear plastic hinged case with 2 or 4 mL vialsConvenient for storing and transporting vialsPartitions and foam inserts protect vials Includes alphanumerical indexing cardChoice of solid or open top screw capCaps attached to vials

The Vial File® provides a compact, easily accessible means of sample storage. The vials are stored in individual partitions within a clear polystyrene case. Foam inserts provide extra protection for the vials. The alphanumerical indexing card allows for identification of sample vials. The Vial File comes with polystyrene case, partitions, indexing card, and 60 – 2 mL vials or 40 – 4 mL vials.

Clear vials are manufactured from Wheaton 33 low extractable borosilicate glass that conforms to ASTM Type I, Class A and USP Type I requirements to protect pH balance and sample integrity. Amber vials are manufactured from Wheaton 320 amber glass that conforms to USP Type I requirements to protect light-sensitive samples. Select from solid or open top black phenolic screw cap with 14B rubber liner for aqueous work and Teflon® faced 14B rubber liner or Teflon faced silicone liner for organic work. Partitions and index cards can also be purchased separately.

Cat. No. Size (mL) *Dia x H (mm) Cap Size No. of Vials Qty/Case

With Clear Vial with Solid Top Black Phenolic 14B Rubber Lined Cap224891 2 12 x 38 8-425 60 1224892 4 15 x 48 13-425 40 1

With Amber Vial with Solid Top Black Phenolic 14B Rubber Lined CapW224896 2 12 x 38 8-425 60 1W224897 4 15 x 48 13-425 40 1

With Graduated Clear Vial with Solid Top Black Phenolic 14B Rubber Lined CapThe 2 mL vial is graduated every 0.2 mL. The 4 mL vial is graduated every 0.5 mL.

W224894 2 12 x 38 8-425 60 1W224895 4 15 x 48 13-425 40 1

With High Recovery Polypropylene Vial with Linerless Polypropylene CapW225100 2 12 x 38 13 mm 60 1

*Measurement is taken with cap attached.

••••••

Vial File for Organic Samples is located on page 153.

153


Sample/Serum

For a complete selection of serum vials see page 20, for aluminum seals and rubber stoppers see pages 32-33.

Cat. No. Size (mL) *Dia x H (mm) Cap Size No. of Vials Qty/Case

With Clear Vials with Solid Top Black Phenolic Teflon® Faced 14B Rubber Lined CapW224693 2 12 x 38 8-425 60 1W224694 4 15 x 48 13-425 40 1

With Amber Vials with Solid Top Black Phenolic Teflon® Faced 14B Rubber Lined CapW224695 2 12 x 38 8-425 60 1W224696 4 15 x 48 13-425 40 1

With Graduated Clear Vials with Solid Top Black Phenolic Teflon® Faced 14B Rubber Lined Cap The 2 mL vial is graduated every 0.2 mL. The 4 mL vial is graduated every 0.5 mL.

W224697 2 12 x 38 8-425 60 1W224698 4 15 x 48 13-425 40 1

With Clear Vials with Open Top Black Phenolic Teflon® Faced Silicone Lined Cap224950 1-1/2 12 x 38 8-425 60 1224952 4 15 x 48 13-425 40 1

With Amber Vials with Open Top Black Phenolic Teflon® Faced Silicone Lined CapW224954 1-1/2 12 x 38 8-425 60 1W224955 4 15 x 48 13-425 40 1

With Graduated Clear Vials with Open Top Black Phenolic Teflon® Faced Silicone Lined Cap The 2 mL vial is graduated every 0.2 mL. The 4 mL vial is graduated every 0.5 mL.

224961 2 12 x 38 8-425 60 1224962 4 15 x 48 13-425 40 1


Clear plastic hinged case for use with 2 and 4 mL vialsConvenient for storing and transporting vialsPartitions and foam inserts protect vials Includes alphanumerical indexing cardExtra partitions and index cards can be purchased separately

The M-T Vial File® provides a compact, easily accessible means of sample storage. The vials are stored in individual partitions within a clear polystyrene case. Foam inserts and partitions provide extra protection for the vials. The alphanumerical indexing card allows for identification of sample vials. The M-T Vial File comes with polystyrene case, partitions, foam inserts and indexing card. Partitions and index cards can also be purchased separately.

Cat. No. Description Stores Qty/Case

Complete Vial File without Vials228778 M-T Vial File 60 – 2 mL vials 6228780 M-T Vial File 40 – 4 mL vials 6

Index Cards228783 For use with 228778 60 – 2 mL vials 35228785 For use with 228780 40 – 4 mL vials 35

PartitionsW228781 For use with 228778 60 – 2 mL vials 6W228782 For use with 228780 40 – 4 mL vials 6

•••••

10 vials in a clear plastic reclosable trayConvenient for storing and transporting vialsVials made from Wheaton 33 low extractable borosilicate glassAttached solid top black phenolic screw cap with 14B rubber linerVials and caps are autoclavable

Clear polystyrene storage case holds 10 vials for convenient storage and transporting of vials. Vials are manufactured from Wheaton 33 low extractable borosilicate glass that conforms to ASTM Type I, Class A and USP Type I requirements. Solid top black phenolic screw caps with 14B rubber liner are attached to vials. Polystyrene case is not autoclavable. Each case contains 5 polystyrene closable trays. Mini-File without vials (Cat. No. 228710) is great for use in shipping or storing vials.


224841 2 1/2 12 x 38 8-425 5224842 4 1 15 x 48 13-425 5228710 — — — — 5


•••••

Cat. No. 228710 without vials

For the Wheaton Uni-Dose® Vial see page 20.

154Vacules


Designed for lyophilization and freeze-drying applications4, 5 and 10 mL sizeScrew threads eliminate use of crimp sealChoice of solid or open top screw cap

Heavy wall construction designed for lyophilization and freeze-drying. Manufactured from Wheaton 33 low extractable clear borosilicate glass that conforms to ASTM Type I, Class A and USP Type I requirements. Vacule vials have controlled ID, for accurate stopper fit. Stoppers and accessories listed below.

Cat. No. Size (mL) Dia x H (mm) Accepts Cap Finish Size Fits Rack Qty/Case

Vacule Vial651954 4 17 x 45 15-425 868810 200651905 5 22 x 40 22-350 868805 200651907 10 22 x 55 22-350 868805 200

Cat. No. Description Qty/Pack Qty/Case

Screw Caps and Stoppers for Cat. No. 651954, 4 mL Vacule224100-080 Red rubber stopper 100 1000224100-093 Lyophilization stopper, 2-Leg 100 1000240209 Cap, solid top, rubber liner, 15-425 screw thread 200 200W240509 Cap, open top, without liner, 15-425 screw thread 200 200

Screw Caps and Stoppers for Cat. No. 651905 and 651907, 5 and 10 mL Vacule224100-172 Red rubber stoppers 100 1000224100-194 Lyophilization stopper, 2-Leg 100 1000224100-202 Lyophilization stopper, 3-Leg 100 1000239853 Cap, solid top, without liner, 22-350 screw thread 500 500

••••

Ideal for

LyophilizationIdeal for

Lyophilization

See our new Lyophilization Section on pages 107-110.

155


Description of Various Types of Tissue Grinders and Recommended Use ...........................156

Preserving Cells with Wheaton Cryule® Cryogenic Vials ........................................... 157-158

Growing Insect and Mammalian Cells with Wheaton MantaRay® Single Use Cell Culture Flask ................................................................................................. 159-160

Primary Culture Preparation with Wheaton Celstir® Cell Culture Flask ...............................158

Utilization of Cell Optimizer System® to Determine Growth Conditions of Mammalian and Insect Cells ...........................................................................................................161

Applications using Wheaton Micro Kits® with Wheaton Connections® ....................... 163-164

How to Use a Socorex Pipette........................................................................................162

Slide Cleaning with Wheaton Staining Dishes .................................................................165

Slide Coating with Silane ..............................................................................................165

Slide Coating with Poly-Lysine .......................................................................................165

There are many methods available to breakdown tissue and cells. Mortar and pestle tissue grinders are commonly used due to their gentle grinding action, which helps to preserve large molecules, organelles, cells, and viruses. General usage and a description of the various types of tissue grinders are described.

Mortar and pestle tissue grinders are commonly used for opening cells to isolate proteins, DNA, RNA and antibodies. They are also used to separate cell organelles and viruses from cells. These are often large molecules or objects that are susceptible to damage. Mortar and pestle tissue grinders are gentler than most other grinding methods, so there is greater recovery of intact molecules, organelles, or viruses. In addition, the gentle grinding makes these tissue grinders useful for breaking tissues down for primary cell culture. Mortar & pestle tissue grinders are easy to use. They consist of only a few parts. Most Wheaton tissue grinders are comprised of interchangeable parts, so there is no concern about matching components for correct sizing after washing in busy labs. This also allows for the easy purchase of replacement parts in case of breakage. Because mortar & pestle tissue grinders are often hand-operated, friction is kept to a minimum, which reduces the amount of heat transferred to the sample, increasing yields of cell products.

Cells collected from cell culture are collected and concentrated if needed. They are ground according to a specific protocol. If a Dounce tissue grinder is used, the protocol refers to a number of strokes.

For other grinders, the time and possibly the RPM are specified for grinding.

When grinding tissues, the sample is trimmed to remove unwanted material. The tissue is chopped using crossed scalpels to a size of 1-3 mm. These pieces are placed in the tissue grinder. Again, a specific protocol that defines the number of strokes or time period is used.

These protocols are determined empirically or a protocol using a similar sample may be used.

Dounce Tissue Grinder Dura-Grind® Stainless Steel Dounce Tissue Grinder

Mortar & Pestle tissue grinders are used for their gentle action, which reduces damage to large molecules, organelles, and viruses or the cells. However, there is a large choice of tissue grinders.

One of the most popular tissue grinders is the Wheaton Dounce tissue grinder, typically used to open cells to release produced molecules, organelles, or viruses. The Dounce tissue grinder is usually used on cell pellets rather than excised tissue. This tissue grinder works by squeezing cells and suddenly releasing pres-sure as the pestle is moved. The Wheaton Dura-Grind® Dounce tissue grinder is constructed of stainless steel, and helps to keep the sample cool. Most Dounce tissue grinders are packaged with two mortars. The loose mortar is used for initial grinding, and the procedure is finished with the tight mortar. The two mortars reduce effort. This grinder is inefficient when the mortar is pressed to the bottom of the pestle and rotated. Dounce tissue grinders are used by moving the pestle up and down for a specified number of strokes.

The Wheaton Potter-Elvehjem tissue grinder is a good general-purpose tissue grinder. It can be used to break excised culture down for primary cell culture. This tissue grinder is generally used with soft tissue such as brain and liver. The serrated Potter- Elvehjem is used to reduce effort because the serrations breakdown the larger pieces first. This tissue grinder works by the shear forces generated by the moving liquid

trapped between the stationary mortar and the moving pestle. Potter Elvehjem tissue grinders with a metal shaft may be used with the Wheaton Overhead Stirrer (903475).

The Wheaton Tapered Tissue Grinder has many uses. Its primary use is to break dissected tissue down. It is commonly used for tougher tissue than the Potter-Elvehjem, such as heart, muscle or lung. The tapered section helps to break down the tissue initially, while the straight portion grinds material via shear forces as in the

Potter-Elvehjem grinder. This tissue grinder works by the shear forces generated by the moving liquid trapped between the stationary mortar and the moving pestle. Tapered tissue grinders with a metal shaft may be used with the Wheaton Overhead Stirrer (Cat. No. 903475).

The Wheaton Tenbroeck tissue grinder has a hollow handle to hold ice water to cool the sample. This feature makes it

useful for labile samples, or for preserving cells such as liver, intestines or heart. In addition to fragile cells, this

grinder is used for the isolation of molecules.

156


157


Cell preservation is an extremely important aspect of cell culture. The only effective means of preserving animal cells is by freezing, using either liquid nitrogen or cryogenic freezers. This product application provides a general description for freezing cells and lists materials needed.

Sterile DMSO or Glycerol (see text for sterilization techniques)

Culture Media

Wheaton Glass or Plastic Cryules®

Preserving cultured cells differs from preserving bacteria and fungi in that higher viability is required. While a 1% survival rate of a microbial culture is practical, such low viability is unacceptable for cell culture. High survival rates are important for cell lines due to the expense and difficulty in preparation, slow rate of growth, and the tendency to change during culture. Consequently, methods used for cell culture cryopreservation must ensure high viability.

Internally threaded Cryule® vials are perceived to hold a better seal because outside dimensions of the cap and vial are equal. This means that under equal temperature, the cap and vial have expanded the same amount, so the seal should be better. Also, internally threaded vials generally have a silicone seal that is perceived to keep liquid nitrogen out of the vial if it is immersed in the liquid. This makes internally threaded vials popular for long term storage. In practice, the contraction going into liquid nitrogen is so small that it doesn’t strain the seal. Although many manufacturers suggest that internally threaded vials can be immersed into liquid nitrogen without harm, many of them sell a sleeve for their vials to prevent liquid nitrogen from entering the seal!

Externally threaded vials, while not as popular for long-term storage, have the advantage that the threads are protected from the environment, reducing the risk of contamination to the culture. The external threading also allows filling to a greater extent than internally threaded vials.

•

•

•

Although some cryogenic vial manufacturers say that there is no trouble immersing cultures into liquid nitrogen, most researchers advise against the practice. Although the vast majority of vials seal under liquid nitrogen, there is a chance that the liquid can seep into the vial over time. When the vial is warmed, the trapped liquid nitrogen rapidly becomes a gas, exploding the vial and sending sharp fragments across the lab. In addition to the danger from fragments, the contents of the vial may also be scat-tered, necessitating decontamination. In addition, frozen bacteria can also seep in with the nitrogen, contaminating the culture. The main advantage to storing cultures in the liquid is the sense of security that the cells will stay cold in the liquid, even if the freezer is opened. It is advisable to store the cultures in the vapor layer above the liquid. The cells will remain cold provided that proper techniques are applied to opening and closing the freezer. A system should be in place so that cultures can be easily found in the freezer, limiting the time the freezer and cultures are disturbed.

Cryoprotectants - choice & sterilization Cryoprotectants such as DMSO and glycerol are used to prevent cell dehydration while freezing. The cell suspension is generally prepared at a concentration twice that desired for freezing so that an equal volume of cryopreservative can be added. Gentle handling techniques during harvest and concentration will greatly enhance the viability of recovered cells. Excessive enzyme treatment, vigorous pipetting, and excessive centrifugation will reduce the viability of the cells. Generally, cell viability should be greater than 90% prior to freezing.

The diffusion of cryoprotective agents such as glycerol or dimethylsulfoxide (DMSO) into a cell will partially replace intracellular water and help prevent dehydration due to ice formation while freezing. Glycerol stabilizes proteins in their native states. The cryopreservative should be prepared separately by combining the cryoprotective agent and the growth medium for the cells.

Cryoprotective agents are usually used individually in concentrations ranging from 5-15% (v/v) with the optimum changing with cell type. Use the highest quality cryoprotective reagents, and sterilize them before use. Glycerol can be sterilized by autoclaving for 15 minutes and stored in small aliquots to prevent contaminants into the bulk material. DMSO is sterilized by filtration with a 0.2 um nylon syringe filter then stored at -20°C in small, single-use sealed aliquots. Air oxidation of DMSO is rapid and these oxidation products are toxic to cells.

Cells mixed with cryopreservative require an equilibration time at room temperature prior to freezing. This equilibration period ranges from 15 to 45 minutes and allows the cryopreservative to penetrate the cell for maximum protection.

Glass Cryules® are sealed with a flame by rolling the sealing neck of the Cryule® in a flame until it becomes soft and pliable. Use forceps to slowly pull the sealing neck of the Cryule® while continuing to roll the tube. As the top of the sealing neck separates from the vial, roll the tip of the Cryule® in the flame to seal. After the Cryule® has cooled, it can be submersed in a solution of methylene blue or trypan blue in order to check that the Cryule® has sealed. Wash the Cryule to remove the stain and examine the cell suspension for the dye. Any dye on the inside of the Cryule® means the vial was not sealed and should be discarded.

The rate of cooling controls the size of the ice crystals and the rate at which they are formed, both of which affect cell recovery. In most cases

158


a slow, uniform cooling rate of -1°C per minute from ambient works well. Placing the vials in a disposable plastic polystyrene rack on the shelf of an -80°C freezer for 2-3 hours is close to 1°C per minute and works well for a range of cell types. After initial cooling, the cells are placed in the storage freezer. For Glass Cryule® vials use Wheaton Plastic Racks on page 142. Tilt the Glass Cryule® in the rack so the culture freezes at a slant. This reduces the chance of the expanding ice cracking the Cryule®. Whether using Wheaton plastic or glass Cryules®, simply leaving the culture in the -80°C freezer overnight is a good way to initially freeze the cells. The temperature at which frozen cells are stored will affect their viability. Storage at -80°C may permit slow chemical reactions to occur from the presence of small amounts of unfrozen water, resulting in cell death. Cells should be stored at a temperature less than -150°C.

Wheaton Science Products Celstir® Cell Culture Flasks are most com-monly used for growing cells in culture, but are widely used to prepare cells, especially neurons, for other cell preparation. Celstir® flasks are useful for preparing cells because they can be closed to maintain sterility of the culture. Jacketed flasks can also be used to maintain the optimal temperature for the cells, saving incubator space and reducing the disturbance to other cultures while preparing primary cultures.

A wide number of researchers have used the Wheaton Celstirs® to prepare cells for other cultures. Generally, the spinner flask is used to gently agitate the cells in culture medium with a trypsinizing agent added. The cells are often prepared with Jacketed Celstir® flasks because of the ease in maintaining optimal conditions for the cells by regulating the temperature of the water flowing through the jacket. Typically, 25-50 mL flasks are used for cell preparation.

Neurons Neurons are adherent cells that do not generally grow in suspension culture. Neurons are generally cultured for a short period of time on slide cover slips or small dishes, where they are manipulated and observed.

Neurons are generally obtained from primary tissue and dissociated to individual cells using a combination of chemical and mechanical techniques.

For example, Martin1 et al placed brain slices from male Spraque-Dawley rats into a Jacketed Celstir® flask containing HEPES buffer, papain, NaCl, KH2

PO4, glucose, glutathione, nitro-arginine, kynurenic acid, and pyruvic

acid. The cells were digested at 36°C by circulating water in the jacket of the flask. The cells were rinsed, and further triturated with Pasteur pipettes with successively smaller tip diameters. This is similar to the technique used by D. James Surmeier2. The cells were dissociated in HEPES buffered Hank’s Balanced Salt Solution. After digestion, the cells were triturated with graded Pasteur pipettes.

Myocytes Haneda3 et al dissociated myocytes in a Wheaton water jacketed Celstir® in a solution containing trypsin, chymotrypsin, and elastase in HEPES buffered Hank’s salt solution at 37°C. The tissue was minced prior to dissociation in the flask.

Decidual Tissue

Tessier4 et al started primary decidual cell culture from tissue obtained from pseudo-pregnant rats. The tissue was incubated in a water-jacketed Celstir® flask at 37°C in RPMI 1640 medium supplemented with collagenase and deoxyribonuclease.

Wheaton Celstir® flasks are an excellent alternative to trypsinizing flasks for small volumes of cell culture. They can be sealed against contamina-tion with the caps that are provided with the flasks, and stirring is done with a magnetic stirrer. The flasks may be placed in an incubator, or jacketed flasks can be used to maintain the cells at optimal temperature. Use of jacketed flasks reduces the demand for incubator space and elim-inates disturbance of other cell cultures from the continual opening and closing of the incubator during primary cell culture preparation. Wheaton Celstir® flasks can be used for a variety of cells and the cultures can be grown in larger Celstir® flasks after the cells are dissociated.

1 Martin, G; Fabre, V; Siggins, G.R.; de Lecea, L Interaction of the hypocretins with neurotransmitters in the nucleus accumbens. Reg. Peptides 104 111-117, 20022 Yan, Z; Surmeier, D.J. D

5 Dopamine Receptors Enhance Zn2+-Sensitive GABA

A Currents in Straital

Cholinergic Interneurons through a PKA/PP1 Cascade. Neuron 19 1115-1126 19973 Oi, S; Haneda, T; Osaki, J; Kashiwagi, Y; Nakamura, Y; Kawabe, J; Kikuchi, K. Lovastatin prevents angiotensin II-induced cardiac hypertrophy in cultured neonatal rat heart cells. Eur. J. Pharmacology 376 139-148, 19994 Prigent-Tessier, A; Tessier, C; Hirosawa-Takamori, M; Boyer, C; Ferguson-Gottschall, S; Gibori, G, Rat Decidual Prolactin – Indentification, Molecular Cloning, and Characterization, Journal Bio Chem, Vol 274, 37982-37989, 1999

In contrast to freezing, rapid thawing of cells is needed to maintain viability. When removing vials from the freezer, insulated gloves should be worn to protect you from burns from the low temperatures. Though specially designed Cryules® are used to store cells, a face shield and a laboratory coat are recommended to protect you against unexpected explosions. Directly after removal from storage, vials should be thawed with agitation, except for fragile hybridoma cells, in a 37°C water bath. As the last ice crystals melt, remove the vial from the water. Wipe, spray, or submerse the vial with 70% ethanol before opening it in a biosafety hood. You can determine the percentage of viable cells recovered by trypan blue staining.

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The MantaRay® is a new and unique cell culture flask for insect and mammalian cells. The flask shows equivalent growth to conventional spinner flasks for both insect and mammalian cells, having been tested on CHO, HeLa, SF-9 cells, 293, and Burkitts lymphoma cells. Data is presented for SF-9 and HeLa cells.

1 ml SF-9 seed was planted in 100 mL media (supplemented with 50 μg/ml gentamycin) in a 250 mL spinner flask and allowed to grow at 27°C (80 RPM). When the cell density was greater than 106 cells/ml, the culture was split to 600 mL in a one-liter spinner flask. The spinner flask was arranged so as to continuously feed fresh 0.2 μm filtered air into the headspace using an air pump, stirring at 80 RPM. When the cell count again reached greater than 106 cells/mL, the culture was split 1:5 by transferring 200 mL culture to 800 mL media that was already temperature equilibrated in two 1 liter MantaRay® flasks and a single 1 liter glass spinner. The three spinners were attached to the air pump via a manifold intended to distribute the air to the headspace of the spinner flasks. Cell count and viability was determined daily using a hemacytometer and trypan blue staining. The cultures were terminated when the viability dropped below 80%.

1 mL Burkitts Lymphoma seed was propagated in RPMI 1640 (supplemented with 10% Fetal Calf Serum and 50 μg/ml gentamycin) at 37°C, 5% CO2

and 80 RPM. It was sequentially split to a volume of 600 mL at which time it was split to the two MantaRay® flasks and one glass spinner as detailed above. The three spinners were attached to the air pump (which was placed inside a 5% CO2

environment) via a manifold intended to distribute the CO

2/air to the headspace of the spinner flasks.

Cell count and viability was determined daily using a hemacytometer and trypan blue staining. The cultures were terminated when the viability dropped below 80%.

SF9 Cells

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CHO-S from Gibco/Invitrogen was used, grown in serum free media from Gibco. These cells are routinely grown in shake flasks at 125 RPM, 37°C, 8% CO2

which is very close to the suppliers instructions. The cells were passaged by diluting them to 2e+5 cells/mL twice a week. The cultures were seeded into both the MantaRay® and a parallel shake flask. A small aquarium pump was used to force incubator air into one of the ports on the top (using a 0.22 μm filter). The other port was used as a vent with a 0.22 μm filter.

FreeStyle 293 from Gibco/Invitrogen was grown in serum free media, also from Gibco. The initial culture was grown in shake flasks at 125 RPM, 37°C, 8% CO2

. The cells were passaged by diluting them to 2e+5 cells/mL twice a week. The cultures were then seeded into both the MantaRay® and a parallel shake flask. A small aquarium pump was used to force incubator air into one of the ports on the top (using a 0.22 μm filter). The other port was used as a vent with a 0.22 μm filter.

The MantaRay® showed similar or superior growth and cell viability compared to standard spinner flasks. The MantaRay® shows equal growth compared to standard glass spinner flasks for both HeLa and SF-9 cells. The MantaRay® has also demonstrated excellent results for CHO, 293, and Burkitts lymphoma cells. The MantaRay® has the advantages of being ready for use out of the package, as it is gamma sterilized and comes complete with a magnet. The MantaRay® is unbreakable, and since it doesn’t require cleaning, saves time and effort in the lab. The unique stirrer provides gentle, ef-ficient mixing, while reducing stress to cells.

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The optimization of growth and production parameters is one of the more critical concerns of cell culture research. Although the most important optimization efforts are directed to the production of desired cellular products, the next most important concern is the production of the maximum number of cells. The Cell Optimizer System® was used to determine the optimal pH growth conditions for the HFN 7.1 hybridoma cell line.

The Cell Optimizer System® was used for the experiments described below. The Cell Optimizer System® is a small (250 mL to 3 L) stirred tank bioreactor. The experimental parameters of pH, temperature, and DO were regulated and controlled using the Control Tower™ equipped with a 4-gas control system. The vessel was stirred with a flat-blade PTFE impeller, using a manually set stirring rate. Data was collected at 10 minute intervals using the BioPro™ data acquisition software.

The HFN 7.1 hybridoma cell line (ATCC, CRL 1606) was used to evaluate a pH optimization protocol. This line was propagated in DMEM/F12 (1:1) (Paragon Biotech, Baltimore, MD) containing 2% FBS (fetal bovine serum). Seed cultures were propagated continually in T-75 flasks in the same medium. The Cell Optimizer System® was filled with 900 mL medium. The environmental parameters were allowed to equilibrate to the following values: 37°C; 50% DO; 50 RPM. The pH was set specific to the study. All cultures were seeded at ~105 cells/mL. Each study was run for 5 days. Cell densities were determined using a hemacytometer.

Optimization of pH for HFN 7.1

HFN 7.1 shows a pH shift of 0.8 to 1 pH units towards acid from a starting pH of 7.4. The cell line appears stable at this low pH, causing some users of this cell line to assume that the optimum pH for this cell line to be pH 6.8. To determine the optimal pH, this cell line was cultured at four pH values ranging from pH 6.8 to 7.4 in 0.2 pH unit increments. The data showed that pH 7.2 provided the maximum growth rate and cell density.

The Cell Optimizer System® can easily be used to design and execute culture optimization protocols, avoiding elaborate setup problems that exist with other bioreactors. This system provides simplicity, but also possesses the full complement of environmental controls. The Cell Optimizer System® was also able to maintain the pH of the culture system, despite the tendency of the culture to shift to a more acidic pH mentioned earlier.

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Pipettes are precision tools that, when used properly, will provide years of trouble-free service. However, many users do not receive training to use this common laboratory utensil.

Your Socorex Pipettes are precision tools that will help you efficiently perform your research and testing tasks. Like any tools, your pipette should be used and stored with care to provide the best possible results.

The information here will apply to pipettes from other manufacturers, as well as your Socorex pipettes. All air displacement pipettes work on the same principle. A plunger is used to displace air- when the plunger is returned to its initial position (upper stop); it creates a vacuum that is used to draw liquid into the disposable tip. The pipette is calibrated such that the vacuum draws a volume of water as indicated on the volume setting. The vacuum is unable to draw liquids heavier than water to the same volume as the same mass of water. Lighter liquids than water are pulled further into the tip, so these liquids will pipette to a larger volume. In the case of either lighter or heavier liquids, compared with water, the volume dispensed will be different than that indicated on the pipette. Pushing the plunger pushes air, which then pushes on the liquid in the tip, which is dispensed.

It is important that you use approved tips with your pipettes to assure accuracy. Using unapproved tips may lead to inconsistent results.

1. Fit the tip, set the volume (variable pipettes only), and press down the pipetting plunger to the first stop (metering stroke) with your thumb. Immerse the tip 2-3 mm in the sample while holding the pipette ertically.

2. Slowly retract the pipetting plunger while watching the liquid fill the tip. You should not observe bubbles or turbulence, which indicate gasses being pulled from the liquid. These gasses reduce the vacuum that draws the liquid, reducing the amount of liquid aspirated into the pipette tip.

3. When the pipetting plunger has been retracted to its upper stop, remove your thumb since the absence of pressure increases the precision of the pipette. Slowly withdraw the pipette from the liquid. Wipe any drops on the outside of the tip on the wall of the vessel you are drawing liquid from.

4. To dispense the liquid, hold the tip against the side of the receiving container at a slight angle. Use your thumb to push down the pipetting plunger to the first stop, and hold it for one second. Wait one second, then push the button to the second stop. Pushing to the second stop blows out any liquid left in the tip.

For work with volatile solvents, such as methanol, you may perform this procedure on a “dummy” sample to saturate the system with vapor. This may improve accuracy, as presaturating the air will reduce the tendency to “blow out” the liquid before you are ready to dispense the sample. You should saturate the tip in this fashion every time you change tips.

Reverse pipetting is used to aspirate an additional volume of liquid. This technique is useful when working with thick, viscous liquids. This is also useful for volatile solvents.

1. Press the pipetting plunger with your thumb to the second stop. This is different from the procedure listed above for regular operation.

2. Holding the pipette vertically, slowly retract the plunger to its upper stop. Wait for the liquid to properly fill the tip. With viscous liquids, this will take longer than when pipetting water. A larger amount of liquid will be aspirated than normal operation since the plunger was pushed to the second stop.

3. When dispensing, push the plunger only as far as the first stop. Wipe any liquid hanging on the tip on the side of the receiving container. Any remaining liquid will be discarded with the tip.

When aspirating the sample, the pipette must be held vertically, or else too much liquid will be drawn in. Tilting the pipette means the vacuum caused by lifting the plunger works less hard to draw liquid, so it draws more liquid than calibrated. Tilting the pipette by 30 degrees causes nearly 1% more liquid to be drawn!

When dispensing the sample, the tip should be held at an angle against the container to draw out the liquid in the tip. Under normal pipetting operations, analytical chemists will recognize the pipette as a “to contain” pipette.

When aspirating the sample, the tip should generally be immersed to 2-3 mm. Placing the tip deeper into the sample allows pressure from the liquid to help push sample into the tip, reducing accuracy.

Under ideal conditions, the sample should the same temperature used to calibrate the pipette. Cold liquids are denser than warm liquids. If the pipette was calibrated at room temperature, but used in a cold-room, smaller samples than expected would be dispensed.

Pipettes should be stored in an appropriate rack or stand. This reduces the risk of scratching or damaging the nose cone. Damage to the nose cone could result in a poor seal to the pipette tip, which will reduce accuracy.

You should periodically check the operation of your pipette by checking its calibration.

Socorex air-displacement pipettes are warranted for calibration for 2 years. Under modern quality management such as GLP/GMP, ISO-9000, or regulatory requirements, you should test your pipettes’ calibration. If your pipette is dropped, or you suspect any type of damage, you should check your pipette’s calibration.

Testing is generally performed gravimetrically, using an analytical balance. Calibration is usually done at room temperature, away from drafts or direct sunlight. The actual calibration details vary between pipettes, and are listed in the pipette instructions.

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An external source of steam is directed to the base of the flask. This can be from an additional boiling flask or a steam line at the bench. Apparatus is useful in isolating essential oils from natural products and can be an alterna-tive to extraction procedures. The Thermometer and Pasteur pipette (Cat. No. 357331) pictured are not included. Uses the following items:

Cat. No. Description

434320 10 mL flask with 20-400 cap125480 Wheaton Connection® 13-425 to 20-400 (2 required)110008 Claison condenser, 13-425 threadsW986294NG 1 mL Graduated V Vial® with 13-425 cap O-Ring included with kit Use the open face cap to hold the Pasteur pipette (357331)(not included) (2 required)WF033112 Connecting adapter, 13-425 screw threads 224952 Cap used to hold thermometer (not included)333927 Water Jacketed condenser, 13-425 threads962515 Hose Connectors (2)125478 Wheaton Connection® 13-425 to 13-425 finish (3 required)168378 Vacuum adapter 13-425 and 20-400 connections

Optional for Vacuum Distillation: Cat. No. Description

962515 3rd Hose Connector, purchased separately (2 Hose Connectors are included in the kit)

Semi-micro mixtures can be distilled using the short path system illustrated. The distillation can be run at atmospheric pressure or under vacuum if necessary. Sub-boiling separations can be effected by heating up to the boiling point, and placing the receiver V Vial® in a cold bath, i.e., dry ice/acetone or liquid nitrogen.

Uses the following items:Cat. No. Description

434320 10 mL flask with 20-400 cap125480 Wheaton Connection® 13-425 to 20-400 (2 required)W986294NG 1 mL Graduated V Vial® with 13-425 cap O-Ring included with kit Use the open face cap to hold the thermometer (not included). WF033112 Connecting adapter, 13-425 screw threads 125478 Wheaton Connection® 13-425 to 13-425 finish168378 Vacuum adapter 13-425 and 20-400 connections962515 Hose Connector

Wheaton Micro Kits are used for a number of different experiments. These kits are successfully employed in both industrial and student lab settings because the apparatus is easy to assemble and use. In addition, disposable sample vials (Cat. No. 224888 or 224952) can be used as both reaction and storage vessels, reducing cleanup costs. A number of different setups are described along with the required materials.

Reflux type reactions may be run for periods of several hours, utilizing a reaction flask (Cat. No. 434320) and a water jacketed condenser (Cat. No. 333927). An air condenser (Cat. No. 265318) may also be used for this assembly. A drying tube having a Teflon® filter and support for the desiccant is illustrated.


434320 10 mL flask with 20-400 cap125480 Wheaton Connection® 13-425 to 20-400333927 Water Jacketed condenser, 13-425 threads962515 Hose Connectors (2)

Optional for Drying Tube:Cat. No. Description

125478 Wheaton Connection® 13-425 to 13-425 finish420836 Teflon® filter with support 265318 Air condenser/drying tube/chromatography column, 50 mm, 13-425 threads

This is the basic setup to distill at atmospheric pressure or under vacuum. This is often referred to as “simple distillation.” If “fractional distillation” is required, air con-denser (Cat. No. 265318) which is packed with stainless steel or glass wool is inserted between the flask and connecting adapter.


434320 10 mL flask with 20-400 cap (2 required)125480 Wheaton Connection® 13-425 to 20-400WF033112 Connecting adapter, 13-425 screw threads W986294NG 1 mL Graduated V Vial® with 13-425 cap O-Ring included with kit

Use the open face cap to hold the thermometer (not included). 333927 Water Jacketed condenser, 13-425 threads962515 Hose Connectors (2)125478 Wheaton Connection® 13-425 to 13-425 finish (2)168378 Vacuum adapter 13-425 and 20-400 connections

Optional for Vacuum Distillation: 962515 3rd Hose Connector, purchased separately

(2 Hose Connectors are included in the kit)

For Wheaton Micro Kits see pages 70-72.

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Claisen adapter (Cat. No. 110008) is inverted and can be used for bulb-to-bulb distillations. The mixture to be separated is placed in a 4 mL vial (Cat. No. 224952) and assembled as shown. The vial is heated but not to the boiling point and condensation promoted by reducing the temperature of the receiver. A small Dewar flask with dry ice/alcohol or liquid nitrogen is used on the receiver side. The thermometer pictured is not included with the kit.


110008 Claisen adapter224952 4 mL vial with 13-425 cap125478 Wheaton Connection® 13-425 to 13-425 thread (2 required)W986294NG 1 mL Graduated V Vial® with 13-425 cap O-ring included with kitWF033112 Connecting adapter, 13-425 screw threads

This classic design is well suited for simple microscale type distillations. The Hickman still has features that make it a good choice for substances having different boiling points. Unlike reflux type reactions, the condensate does not return to the flask; it is retained in the alembic portion of the still. An air condenser may be attached to the upper sec-tion to increase the area for condensation if needed.


W986299NG 5 mL Graduated V Vial® with 20-400 cap125480 Wheaton Connection® 13-425 to 20-400620189 Hickman still, 13-425 and 20-400 connections Cap from 986299 to hold thermometer O-ring to hold thermometer (thermometer not included)

Concentration of solute may be done within the V Vial®, avoiding a transfer. Connect the vacuum adapter (Cat. No. 168378) with a connector and attach hose connectors as shown. Introduce nitrogen or argon through the uppermost fitting and use the other fitting for venting. Solvent can be recovered if PTFE tubing is coiled within an ice bath. Uses the following items:

Cat. No. Description

W986294NG 1 mL Graduated V Vial® with 13-25 cap125480 Wheaton Connection® 13-425 to 20-400168378 Vacuum adapter with 13-425 and 20-400 threads962515 Hose Connectors, 13-425 threads (2 required)

Small quantities of reactant can be added to the boiling flask using a syringe (not included). Solvent vapors are condensed and collected in the receiver flask. This operation is similar to a rotary evaporator. Uses the following items:Cat. No. Description

W986294NG 1 mL Graduated V Vial® with 13-425 cap125478 Wheaton Connection®, 13-425 to 13-425 (3 required)434320 10 mL flask with 20-400 cap125480 Wheaton Connection®, 13-425 to 20-400333927 Water jacketed condenser, 13-425 connection962515 Hose connectors, 13-425 connection (2 required)

Optional for Drying Tube:Cat. No. Description

125478 Wheaton Connection®, 13-425 to 13-425265318 Air condenser/drying tube/column 13-425 connections420836 Teflon® filter with support (holds desiccant)

volumes less than 1.0 mLMicro product V Vial® is used with an air condenser (Cat. No. 265318 or 265320) with connector (Cat. No.125478) and magnetic stirrer to react and condense the solvent in the extended upper portion of the vial. Biological products may be attached to the freeze dryer if the Teflon®; support and filter (Cat. No. 420836) are used within the connection. Small volumes can be centrifuged within the vial if necessary.


W986294NG 1 mL Graduated V Vial®

125478 Wheaton Connection®, 13-425 to 13-425265318 Air condenser/drying tube/column, 50 mm, 13-425 connection265320 Air condenser/drying tube/column, 100 mm, 13-425 connection

Optional for Freeze Drying:Cat. No. Description

420836 Teflon® filter with support cap from 986294

AbstractCoating slides with Poly-Lysine allows binding of DNA and certain proteins. A Poly-Lysine coating also promotes cell adhesion to slides for culturing or experiments, such as calcium ratio imaging. Wheaton staining dishes offer a convenient method for efficiently coating a number of slides.


30-Slide Rack, Stainless Steel 2 900234Staining Dish & Cover 4 900303Poly-Lysine solution (0.1% w/v) 70 mL Available commerciallyTissue Culture PBS 70 mL —Double-Distilled water 2 L —Amber HDPE bottles 1000 mL 209130Drying Oven* 1 — Orbital shaker 1 — Centrifuge 1 — Dust-free plastic containers to hold slides —

*45°C - vacuum oven preferred

ProcedurePrepare the poly-lysine by adding 70 mL of poly-lysine to 70 mL of PBS solution and 560 mL water. Store in an amber HDPE bottle (209130).

Clean slides as described in the Slide Cleaning Product Application Note. Place an equal number of slides in each of two stainless steel slide racks.

Transfer slides to the polylysine solution and shake for 15 minutes to 1 hour.

Transfer the racks of slides to fresh staining dishes filled with fresh double-distilled water. Plunge up and down at least 5 times to rinse the slides.

Centrifuge slides on a microtiter plate carrier at 500 RPM for 5 minutes. Place paper towels under racks to absorb moisture. Use two racks of slides to balance the centrifuge.

Place the racks of slides in clean staining dishes with covers for transport to vacuum oven. Place the dishes in the oven at 45°C for 10 minutes. Vacuum is optional.

Store the slides in a plastic slide box without a rubber mat bottom.

Numerous users commented that slides should be stored for 14 days before spotting DNA. Slides are best when used within 4 months.

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AbstractClean microscope slides are important for all areas of microscopy. Even pre-cleaned slides can carry a significant amount of oil or lint, even from a freshly opened box.

Wheaton staining dishes offer a convenient method to efficiently clean a number of slides as an initial preparation for coating slides or for microscopy.


Glass Microscope Slides 60 —30-Slide Rack, Stainless Steel 1 900234Staining Dish & Cover 2 900303Double Distilled Water 5 liters — Solid NaOH 70 g — 95% Ethanol 420 mL — Drying Oven* 1 — Orbital Shaker 1 —

*45°C-vacuum oven preferred

ProcedurePrepare the cleaning solution by dissolving 70 g NaOH in 28 mL double distilled water. Add 420 mL 95% ethanol. If the solution is cloudy, add double distilled water until the solution clears.

Place the slides in a stainless steel rack and the rack in the staining dish. Pour the cleaning solution from Step 1 over the slides so the slides are covered. Cover the staining dish with a glass lid, and put the staining dish with slides on the orbital shaker to mix for 2 hours.

Transfer the slides to a fresh staining dish filled with double distilled water. Rinse the slides by plunging them in the water several times. Repeat the rinse at least 4 times, using fresh double distilled water for each rinse. It is critical to remove all traces of Ethanol-NaOH.

If you are not coating the slides, place the slides in a slide rack in an oven at 45°C. Use of a vacuum oven is optional. Protect the slides from dust in the air by rinsing all glassware prior to use, and by keeping all solvents, slides and staining dishes covered.

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AbstractSilane coatings are used for paraffin sections, frozen sections and in situ hybridization. A quick & easy procedure is provided to coat microscope slides with a silane coating.


Staining Dish & Cover 3 90030320 Slide Rack, Glass 3 9003042% 3-aminopropyltriethoxysilane (AES) in acetone* 500 mL — Double-Distilled water 500 mL — Acetone 500 mL —

*Keep this in a fume hood

ProcedurePrepare 2% 3-aminopropyltriethoxysilane (AES) in

acetone (do this under a fume hood).

Clean slides as described in the Slide Cleaning Product Application Note.

Place slides in a slide rack, and rinse slides in a staining dish with acetone. Move the rack of slides to a staining dish containing 2% AES in acetone. (Note - dedicate this staining dish for the AES solution. Do not use it for anything else). The slides will need 5 to 15 minutes in this solution.

Rinse slides by dunking 4 times in fresh acetone. Repeat this wash step 2 times with fresh acetone.

Rinse slides by dunking them into a staining dish containing fresh double distilled water.

Dry slides overnight in a dust free environment. They can be dried in a vacuum oven. These slides can be kept for up to 6 months in a dust-free plastic box.

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We compiled this section of the catalog to give our customers more technical information on our products. If you need more information than what’s listed in these pages, please contact our Technical Service staff.

.......................................... Inside Back Cover

Determining a Cap’s Thread Finish ....................................................... 167 Guide For Selecting A Cap Liner .......................................................... 169Rubber Stopper Formulation Characteristics .......................................... 170 Rubber Stopper Formulation Descriptions ............................................. 171Suggested Torque for Screw Caps ........................................................ 167

Determining a Glass Container Thread Finish ........................................ 175 Factors for Selecting a Glass Container ................................................. 174General Cleaning of Glassware ............................................................. 179Glass Manufacturing Terminology ......................................................... 172Glass Types ........................................................................................ 173Mold Lubricants and Residues ............................................................. 177Pressure and Vacuum in Glass Vessels ................................................. 177Recycling Glass Containers .................................................................. 179Sterilization of Glass Containers ........................................................... 177Weathering of Glass Containers ............................................................ 177

Autoclave & Sterilization Recommendations .......................................... 178 Labeling Adhesives for Coated Glass Containers .................................... 178Recycling Safety Coated Containers ...................................................... 178

Biological Properties of Plastics ............................................................ 183Determining a Plastic Container Thread Finish ....................................... 183Factors for the Selection of Plastic Containers ....................................... 182Plastic Manufacturing Terminology ....................................................... 180Plastic Resins ..................................................................................... 181Resin Identification Codes .................................................................... 187Sterilization of Plastics ......................................................................... 185

Steve Smith, a 35 Year Veteran of Wheaton Kirsti Dare, a 28 Year Veteran of Wheaton



Wheaton Science Products is a leading supplier of glass containers for the laboratory. We understand well the importance of protecting the quality of the most sensitive materials. We offer a broad range of both molded and tubing based glass containers and vials. Over the years we have developed thousands of containers of different configurations and sizes. Containers and vials can be supplied in different glasses, clear and amber with different neck finishes both with and without closures for your many applications. If a standard container doesn’t exist to fit your unique needs, we can probably create one to your specification.

We also offer a broad range of plastic containers and vials for the laboratory and diagnostics markets. We are able to provide products that have been produced to extreme tolerances like our Wheaton Leak- Resistant plastic bottles and caps, as well as precision items such as our controlled dropper tips. We have over 40 years of plastic manufacturing experience and our mold design and production capabilities provide you with the most efficient and economical products for your lab.

Wheaton Science Products has developed and produced a variety of unique laboratory instruments that are designed to facilitate routine tasks and automate complex processes. These products include automated liq-uid dispensers and laboratory equipment for cell culture development and scale-up systems for cell culturing. In addition, Wheaton Science Products can custom design and produce instrumentation to specification. We can take a project from concept through production and provide complete control from start to finish. GMP assembly and testing procedures are performed by highly skilled technicians using state-of-the-art equipment.

Your Source for Glass and Plastic Containers and Laboratory Equipment

167


Caps & Closures

T he closure industry has not standard-ized on dimensions to the extent that

the container industry has, and it is usually advantageous to buy both container and closure from the same supplier when possible. Similar to the container industry, when a closure finish is designated as 33-400, it means that the nominal diameter measured across the inside of the cap at the opening is approximately 33

mm. (See “T” dimension on illustration.) The 400 designates a specific style of thread. The thread finish of the cap and container must be the same. A container with a 33-400 thread finish should be used with a cap that has a 33-400 thread finish.

To determine the cap size, measure the cap opening from one side of the inner wall to the opposite side of the inner wall. Compare this number to the numbers found in the “T” dimension columns in Table 2a or 2b. Once this number is found in the table, follow the row to the far left to find the “Nominal Diameter” of the cap (33 in the above example).

To determine the specific style of thread, measure the depth of the cap from the liner surface to the outside edge of the cap. Compare this number to the numbers found in the “H” dimension columns in Table 2a or 2b that appear in the same row as the Nominal Diameter of the cap. Once this number is found in the table, follow the column to the top to find the specific style number (400 in the above example). The dimen-sions in the tables are approximate and will probably be slightly different from what is measured (especially the “H” dimension due to variations in liner thickness), but should be close enough to allow for the proper determination of the cap size.

The integrity of the cap-to-container seal is dependent upon a number of variables, such as the materials of the cap, liner, and container, the sealing surface of the container, and the application torque applied to the closure. The most important of these is the application torque. If the cap is applied too loosely, the contents could leak (especially during shipping). If the cap is applied too tightly, it may be too difficult to remove the cap, or the container could break during application.

(in-lb)

Cap Application Min Removal Application Min Removal Application Min Removal Application Min Removalmm Torque Torque Torque Torque Torque Torque Torque Torque

15 8 4 6 3 12 7 8 418 9 5 7 4 13 8 9 520 10 5 8 4 15 9 10 522 11 6 9 5 17 10 11 524 12 6 10 5 18 11 12 628 14 7 12 6 21 12 14 733 18 9 15 7 24 14 17 838 20 10 17 7 29 17 19 943 22 11 18 9 33 20 22 1148 24 12 20 10 36 22 24 1258 28 14 24 12 44 26 29 1470 35 18 28 14 52 32 35 1789 45 22 36 18 65 40 45 22100 50 25 40 20 75 38 50 25

Table 1 offers some suggested torques that should provide an adequate seal for most applications. It is recommended that proper tests be performed to determine the optimum torque for the application. The most practical way to check the tightness is to measure the removal torque after the cap has been on the container for about 5 minutes. The removal torque should closely approximate the application torque. The minimum removal torque noted in the table should be maintained after a 24 hour period.

Although the information in this chart was acquired from reputable sources, it should only be used as a guide in determining the proper application torque. Wheaton accepts no responsibility for the accuracy of this data or for any consequences resulting from its use.

For a complete selection of Wheaton closures see the Cap & Closure Section on pages 31-42.If you need additional assistance on closures contact our Technical Service Department.

168 Caps & Closures


(Dimensions are in inches)

Nominal 400 410 415 425 430Dia (mm) “T” “H” “T” “H” “T” “H” “T” “H” “T” “H”

8 — — — — — — 0.360 0.245 — —10 — — — — — — 0.415 0.255 — —13 — — — — 0.520 0.430 0.520 0.280 — —15 — — — — 0.585 0.535 0.585 0.280 — —18 0.790 0.360 0.710 0.500 0.710 0.595 — — 0.710 0.60520 0.790 0.360 0.790 0.530 0.790 0.720 — — 0.790 0.60522 0.870 0.360 0.870 0.560 0.870 0.815 — — 0.870 0.60524 0.945 0.390 0.945 0.620 0.945 0.935 — — 0.945 0.65028 1.095 0.390 1.095 0.685 1.095 1.060 — — 1.095 0.72530 1.130 0.390 — — — — — — 1.130 0.76033 1.270 0.390 — — — — — — 1.270 0.77535 1.370 0.390 — — — — — — — —38 1.480 0.390 — — — — — — 1.480 0.94040 1.590 0.390 — — — — — — — —43 1.660 0.390 — — — — — — — —45 1.750 0.390 — — — — — — — —48 1.875 0.390 — — — — — — — —51 1.975 0.390 — — — — — — — —53 2.075 0.390 — — — — — — — —58 2.230 0.390 — — — — — — — —60 2.350 0.390 — — — — — — — —63 2.470 0.390 — — — — — — — —66 2.585 0.390 — — — — — — — —70 2.745 0.390 — — — — — — — —75 2.920 0.390 — — — — — — — —77 3.040 0.470 — — — — — — — —83 3.275 0.470 — — — — — — — —89 3.520 0.515 — — — — — — — —100 3.945 0.580 — — — — — — — —110 4.340 0.580 — — — — — — — —120 4.735 0.675 — — — — — — — —

(Dimensions are in millimeters)

Nominal 400 410 415 425 430Dia (mm) “T” “H” “T” “H” “T” “H” “T” “H” “T” “H”

8 — — — — — — 9.14 6.22 — —10 — — — — — — 10.54 6.48 — —13 — — — — 13.21 10.92 13.21 7.11 — —15 — — — — 14.86 13.59 14.86 7.11 — —18 18.03 9.14 18.03 12.70 18.03 15.11 — — 18.03 15.3720 20.07 9.14 20.07 13.46 20.07 18.29 — — 20.07 15.3722 22.10 9.14 22.10 14.22 22.10 20.70 — — 22.10 15.3724 24.00 9.91 24.00 15.75 24.00 23.75 — — 24.00 16.5128 27.81 9.91 27.81 17.40 27.81 26.92 — — 27.81 18.4230 28.70 9.91 — — — — — — 28.70 19.3033 32.26 9.91 — — — — — — 32.26 19.6935 34.80 9.91 — — — — — — — —38 37.59 9.91 — — — — — — 37.59 23.8840 40.39 9.91 — — — — — — 43 42.16 9.91 — — — — — — — —45 44.45 9.91 — — — — — — — 48 47.63 9.91 — — — — — — — —51 50.16 9.91 — — — — — — — —53 52.71 9.91 — — — — — — — —58 56.64 9.91 — — — — — — — —60 59.69 9.91 — — — — — — — —63 62.74 9.91 — — — — — — — —66 65.53 9.91 — — — — — — — —70 69.72 9.91 — — — — — — — —75 74.17 9.91 — — — — — — — —77 77.22 11.94 — — — — — — — —83 83.19 11.94 — — — — — — — —89 89.41 13.08 — — — — — — — —100 100.20 14.73 — — — — — — — —110 110.23 14.73 — — — — — — — —120 120.27 17.14 — — — — — — — —

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Caps & Closures

Usually the smallest component part of the package and usually overlooked is the selection of the cap liner. The liner must not alter or be altered by the product. It must withstand repeated applications and removals against the container surface while maintaining the integrity of the sealing surface. Below is some information that may help in choosing the right liner from our product offering.

Material Description Applications

Poly-Vinyl One mil poly vinyl film bonded to one mil HDPE on a #30 white pulp paper backing. Superior to plain pulp paper because it provides excellent moisture barrier.

General purpose: Suitable for wide range of applications. Chemical resistance: Good for mild acids, alkalis, solvents, alcohols, oils and aqueous products; poor for active hydrocarbons and bleaches.

General Purpose: Broad applications base. Chemical resistance-good for acids, alkalis, solvents, alcohols, oils, household cosmetics and aqueous products. Poor for hydrocarbon solvents. Liner provides tight seal.

Poly-Seal® Manufactured from LDPE. The unique cone design provides a wedge type seal that not only seals across the top but also across the inside diameter.

Unique problem solving type of liner. This liner is stress crack resistant and offers superior torque retention and excellent sealing characteristics. It is recommended that this liner be tested prior to use for leak seal.

Foamed Polyethylene A one piece, three ply coextruded liner consisting of foamed and solid LDPE. The foam core is sandwiched with solid clear PE.

Pulp/Metal Foil


Styrene-Butadiene Rubber/0.005 PTFE

Teflon Faced Silicone Rubber

Teflon Faced Foamed Polyethylene

The white rubber lining material consists of homoge-neous sulfur cured styrene-butadiene rubber (SBR). FDA Status complies with 21CFR 177.26, “Rubber articles intended for repeated use.”

The white rubber/0.005” PTFE liner consists of virgin PTFE bonded to the white sulfur cured, styrene-butadiene rubber. Complies with the FDA 21CFR 177.1550.

The liner consists of 0.005” thick Teflon bonded to 0.055” thick silicone rubber.

Teflon®-faced foamed polyethylene liner that offers the excellent chemical resistance of Teflon® with the com-pressibility and sealing properties of polyethylene foam.

Aluminum foil bonded to pulp board. Good barrier properties, good resistance to hydrocarbons, oils, ketones and alcohols. Not good for acids or alkalis.


Designed for the ultimate in product safety. PTFE provides totally inert inner seal and surface facing the sample or product. Autoclav-able.

Ideal for low temperature storage applications. Teflon facing provides excellent chemical barrier. Autoclavable


Note: Closures and liners are designed for a variety of applications. Product performance can vary depending on conditions. It is recommended that proper tests be performed to determine the best liner for the application.

170 Caps & Closures


Listed below are the primary stopper formulations with general descriptions that are supplied by Wheaton Science Products.

Pros: low gas and vapor permeability, good for multiple piercing applications, compatible with most cephalosporin’s, resistant to animal, vegetable, and mineral oils, good resistance to aliphatic, aromatic and chlorinated solvents. Cons: not recommended for use with ketones. Can be autoclaved and irradiated.

Pros: low gas and vapor permeability, good for multiple piercing applications, excellent moisture absorption and desorption properties following autoclave and lyophilization drying cycles. Cons: poor resistance to mineral oil, aliphatic, aromatic, and chlorinated solvents. Can be autoclaved and irradiated.

Pros: low gas and vapor permeability, great for multiple piercing applications after gamma irradiation, ultra low extractable compound, compatible with most cephalosporin’s, very good moisture absorption and desorption properties following autoclave and lyophilization drying cycles, compatible with WFI applications. Cons: poor resistance to mineral oil, aliphatic, aromatic, and chlorinated solvents. Can be autoclaved and irradiated.

Pros: low gas and vapor permeability, very good properties regarding ozone, animal and vegetable oil. Cons: not good for multiple piercing applications, poor resistance to mineral oil, aliphatic, aromatic, and chlorinated solvents. Can be autoclaved.

Pros: low gas and vapor permeability, good for multiple piercing applications, resistant to animal and vegetable oils. Cons: poor resistance to mineral oil, aliphatic, aromatic, and chlorinated solvents. Can be autoclaved and irradiated.

Pros: low gas and vapor permeability, good for multiple piercing applications, resistant to animal and vegetable oils, good for lyophilization applications. Cons: poor resistance to mineral oil, aliphatic, aromatic, and chlorinated solvents. Can be autoclaved and irradiated.

Pros: low gas and vapor permeability, resistant to animal and vegetable oil, good for lyophilization applications. Cons: poor resistance to mineral oil, ali-phatic, aromatic, and chlorinated solvents. Can be autoclaved and irradiated.

Pros: low gas and vapor permeability, resistant to animal and vegetable oil, good for lyophilization applications. Cons: not good for multiple piercing applications, poor resistance to mineral oil, aliphatic, aromatic, and chlorinated solvents. Can be autoclaved and irradiated

Pros: barrier properties of PTFE, good coring characteristics, fair resistance to gas and moisture transmission compared to red isoprene. Cons: contains dry natural rubber. Can be autoclaved.

Pros: good coring and reseal characteristics, fair resistance to gas and moisture transmission compared to red isoprene, good for lyophilization applications. Cons: contains dry natural rubber. Can be autoclaved.

Pros: good resistance to gas and vapor transmission, good for lyophilization applications. Cons: poor coring and re-seal characteristics, not good for use with acidic products. Can be autoclaved.

Pros: good coring and re-seal characteristics. Cons: contains dry natural rubber, poor resistance to gas and vapor transmission compared to butyl, not appropriate for products that require an inert gas blanket, not good for use with acidic products or solvents. Can be autoclaved and irradiated.

Pros: good coring and re-seal characteristics. Cons: contains dry natural rubber, poor gas and vapor transmission compared to butyl, not appropriate for products that require an inert gas blanket, not good for use with acidic products or solvents. Can be autoclaved and irradiated.

Pros: good coring and re-seal characteristics. Cons: contains dry natural rubber, poor gas and vapor transmission compared to butyl, not appropriate for products that require an inert gas blanket, not good for use with acidic products or solvents. Can be autoclaved and irradiated.

Pros: Good for high heat applications, can withstand multiple steam autoclaves. Cons: very poor barrier to gas and vapor transmission, not appropriate for products that require an inert gas blanket. Can be autoclaved and irradiated.

Pros: low gas and vapor permeability, resistant to animal, vegetable and mineral oil, aliphatic, aromatic, and chlorinated solvents, good for high heat applications. Cons: not recommended for ketones. Can be autoclaved.

2-Leg Lyophilization Stoppers3-Leg Lyophilization StopperStraight Plug Style

Gray Bromobutyl/39 Gray Gray Gray Gray Gray Gray Grayw/Safety Coat Bromobutyl/46 Bromobutyl/47 Bromobutyl/50 Chlorobutyl/45 Chlorobutyl/46 Chlorobutyl/50 Chlorobutyl/55

Typical Properties: Base Polymer Bromobutyl Bromobutyl Bromobutyl Bromobutyl Chlorobutyl Chlorobutyl Chlorobutyl Chlorobutyl

Durometer (shore A +/- 5) 39 46 47 50 45 46 50 55Specific Gravity 1.19 1.35 1.26 1.35 1.24 1.32 1.30 1.38Ash % 31 48 41 44.9 38.4 45.5 42.7 47.9

Typical Extraction Data, USP: Distilled Water pH Change - 0.3 - 0.12 0.00 + 0.22 - 0.20 - 0.37 - 0.23 - 0.30Reducing Agents, ml, .01N I2 0.00 0.00 0.01 0.04 0.00 0.00 0.00 0.00Turbidity, NTU 0.01 0.70 0.03 1.40 1.00 0.40 0.05 1.00Total Solids, mg/100 mL 0.8 0.7 0.3 1.3 0.0 0.3 0.4 1.0Zinc, ppm — — — 1.31 1.20 — 0.20 0.30Lead, ppm — — — < 0.50 < 0.50 — < 0.50 < 0.50

Toxicity Data: Acute Systemic Passes — — — Passes — Passes PassesIntracutaneous Reactivity Passes — — — Passes — Passes PassesCytotoxicity Passes Passes Passes Passes Passes Passes Passes Passes

Gray Chlorobutyl Gray Chlorobutyl Black Red Red Pink Natural BlackIsoprene Blend/40 Isoprene Blend/50 Halobutyl/60 Isoprene/45 Natural/40 Natural/48 Silicone Viton/55

Typical Properties: Base Polymer Chloro/Iso Blend Chloro/Iso Blend Halobutyl Isoprene Natural Natural Silicone Viton

Durometer (shore A +/- 5) 40 50 60 45 40 48 55 55Specific Gravity 1.15 1.46 1.43 1.33 1.25 1.65 1.14 1.87Ash % 29.5 55.7 51.2 32.0 38.9 55.6 N/A N/A

Typical Extraction Data, USP: Distilled Water pH Change - 0.6 - 0.8 - 0.3 - 0.2 + 0.1 + 1.5 - 0.30 0.38Reducing Agents, ml, .01N I2 0.0 0.0 0.0 0.00 0.0 0.0 0.10 0.06Turbidity, NTU 0.7 2.6 5.0 0.90 29.0 34.0 1.00 0.00Total Solids, mg/100 mL 0.0 0.0 0.5 1.6 1.4 1.9 0.0 0.4Zinc, ppm 1.0 1.6 0.5 2.50 0.2 0.3 — <0.05Lead, ppm 0.0 — 0.0 < 0.50 0.0 0.1 < 0.50 < 0.50

Toxicity Data: Acute Systemic Passes Passes Passes Passes Passes Passes Passes —Intracutaneous Reactivity Passes Passes Passes Passes Passes Passes Passes —Cytotoxicity Passes Passes Passes — — — Passes Passes

All stopper formulation data presented above is general information. Specific laboratory results are available upon request. It is recommended that all stoppers be thoroughly tested by the customer for compatibility.

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Caps & Closures

172Glass


The temperature at which internal stresses in glass are significantly reduced. In the annealing operation, glass is gradually cooled from above the annealing point temperature to below the strain point temperature. This slow cooling relieves residual thermal stresses that would develop if the glass were allowed to cool in an uncontrolled manner.

The mixed raw materials used in manufacturing glass that have been blended and proportionally mixed for delivery to the glass furnace.

Usually refers to a glass parison that is formed during the first step of glass molding. The piece is then transferred to a lamp worker or glass blower for final shape configuration.

A gaseous inclusion or bubble in the glass.

Usually a metal mold used to form a piece of glass from a hot gob.

A high silicate glass that has at least 5% boron oxide.

The fractional change in length of a piece of glass per degree change in temperature on cooling from the annealing point to ambient temperature.

Waste or broken glass. Clean cullet is always used in the batch.

Mass per unit volume measured in grams per cubic centimeter.

The wall thickness or the evenness of the glass distribution throughout the container.

To attack the glass surface with a strong chemical agent, usually hydro-fluoric acid. Usually used in decorating glass.

The part of a bottle which holds the cap, stopper or closure. The area that has the threads (generally a shortened term for thread finish). The first part made on an automatic machine, but the last part (or finish) to be made when bottles were hand blown. On labware, may refer to an interchangeable ground joint.

The shaping of hot glass.

The shaping of glass using air pressure.

A portion of hot glass that is delivered from the furnace for forming.

A glass with a high softening point or high viscosity (usually borosilicate).

A manufacturing term for the area of a glass manufacturing plant where molten glass is processed.

Flame re-working of a blank or tubing cane, typically on a lathe.

A long belt-fed, tunnel-shaped oven used to heat glass to the annealing point and then slowly cool it to room temperature to remove any residual thermal stresses in the glass. Can also be a large oven where glass is manually loaded and unloaded (batch lehr).

The fractional change in length of a piece of glass per degree change in temperature. The coefficient of expansion generally indicates the thermal endurance of the glass. Glasses with a low linear coefficient of expansion can be subjected to greater rapid temperature changes with less chance of fracture than glasses with a high coefficient of expansion. (Generally, Type I glasses have a lower COE than Type III).

The amount of glass that is melted at one time.

The mark in the bottom of the container that denotes the manufacturer.

Glassware that is formed by pressing a gob between a mold and a plunger.

A glass with a substantial portion of lime in the formula.

Temperature at which a thread or rod of glass rapidly deforms under its own weight.

The temperature at which thermal residual stresses become permanent upon cooling. Temperatures above the strain point will introduce permanent stresses that can cause or contribute to fracture. At temperatures below the strain point, the glass can be temporarily heated and cooled without introducing permanent stress. The strain point can be considered the maximum service temperature.

The furnace that melts the raw materials into molten glass. Temperatures in the tank vary depending on the glass type being melted, but are typically in excess of 1,200°C.

The degree of residual stress in annealed glass as measured using polarized light techniques.

The attack on glass surface by atmospheric elements.

173


Glass

The glass products in this catalog are made from many different glass formulations. Following are brief definitions of these glass types and descriptions of their characteristics.

180 Glass: An exceptionally clear borosilicate glass of high chemical durability, which has been especially formulated for the lowest background count. Great care has been taken to select only those ingredients for the batch that would not cause unwanted background count or color. Potassium as a separate element has been excluded from the batch to minimize K40. Special controls assure high quality and batch to batch uniformity. This glass is only available as a tubing vial.

200 Glass: Also referred to as 33 expansion low extractable borosilicate glass, is a clear borosilicate glass with exceptional thermal endurance that glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements. Most of our laboratory products are made from this type of glass. This glass meets all sterilization requirements.

300 Glass: A chemically resistant clear borosilicate glass that meets all the requirements for Type I glass as specified in the current revision of the U.S. Pharmacopeia.

320 Glass: A similar composition to 300 Glass except amber color for light sensitive applications. Meets UV light protection limits as specified in the current revision of the U.S. Pharmacopeia.

400 Glass: A clear borosilicate glass that falls well within the limits for USP, Type I chemically resistant borosilicate glass, as specified in the current revision of the U.S. Pharmacopeia.

500 Glass: Similar to the 400 Glass formulation except amber color for light sensitive applications. Meets UV light protection limits as specified in the current revision of the U.S. Pharmacopeia.

800 Glass: A superior soda-lime clear glass that meets requirements for Type III soda lime glass as specified in the current revision of the U.S. Pharmacopeia.

900 Glass: Similar in formulation to 800 Glass except amber color for light sensitive applications. Meets UV light protection limits as specified in the current revision of the U.S. Pharmacopeia.

USP Glass Type Si Al Na K Ca Mg Fe Ba Zn Ti

Type I Clear - Molded 1.3 <0.006 0.26 0.05 <0.02 <0.02 <0.015 <0.008 0.14 <0.005Type II Clear - Molded 1.65 <0.006 0.5 0.04 <0.02 <0.02 <0.015 <0.008 0.16 0.007Type III Clear - Molded 26 0.17 9.1 0.18 3.6 0.20 <0.015 <0.008 0.07 <0.005Type I Amber - Molded 2.4 0.06 0.71 0.1 <0.02 <0.02 <0.015 <0.008 0.21 <0.005Type II Amber - Molded 1.4 0.18 9.5 0.27 3.4 0.23 0.2 <0.008 0.18 <0.005Type III Amber - Molded 22 0.16 9.3 <0.03 3.4 0.23 0.016 <0.008 0.03 <0.005Type I Clear - Tubular 4.8 <0.01 1.1 <0.03 0.13 0.07 <0.012 <0.008 <0.01 <0.005Type I Amber - Tubular 7.4 0.17 1.8 0.45 <0.02 <0.02 <0.015 <0.008 0.23 <0.005Blank <0.05 <0.006 <0.01 0.048 <0.02 <0.02 <0.015 <0.08 <0.006 <0.005

Results after autoclave at 121° C for 60 minutes - high purity water in 20 mL containers

“180” “200” “300” “320” “400” “500” “800” “900”

Strain Point °C 510 505 525 510 520 505 510 496

Annealing Point °C 560 560 570 560 560 540 548 536

Softening Point °C 821 820 785 770 735 730 729 713

Linear Coefficient of Expansion

(0-300°C)x10-7 33 33 55 55 63 62 88 91

Density g/cm3 2.23 2.22 2.33 2.42 2.44 2.46 2.48 2.50

ASTM Glass Type I I I — — — — — & Class A A B — — — — —

USP Glass Type I I I I I I III,NP III,NP

Light Protection Yes Yes Yes

NP= Non-parenteral USP= U.S. Pharmacopeia

“180” “200” “300” “320” “400” “500” “800” “900”

Forming Tubing Tubing Tubing Tubing Molded Molded Molded Molded Process Vial Vial Vial Vial Container Container Container Container

Si02 81 81 72 70 69 65.5 73 73

Al2O3 2 2 7 6 5.5 6 2 2

Na2O+K2O 4 4 9 8 10 9 14 14.2

CaO+MgO <0.2 <0.2 1 0.5 1.5 0.5 10.5 10

B2O3 13 13 12 7 11 9 0.5

Fe2O3 <0.1 <0.1 <0.1 1.5 <0.1 1 <0.05 0.3

BaO <0.1 2 2.5 1.5

ZnO 0.5 0.5

MnO2 7

TiO2 5

SO3 0.2

174Glass


Chemical durability and USP type glass surfaces are subject to attack when exposed to solutions and vapors with which they can react. Reactive substances will leach constituents from the glass surface into the contained product. This reaction will occur with ordinary aqueous, saline and alcohol based solutions. The primary ion removed from the glass surface is sodium; however, all elements are subject to leaching. It is not uncommon to experience an increase in product pH as sodium is extracted from the container. The corrosion of glass occurs over time, and is accelerated by wet or moist heat-treat processes such as autoclaving.

The United States Pharmacopoeia classifies pharmaceutical glass containers according to their resistance to water attack, also referred to as chemical durability. Containers are classified as Type I, II, III and NP. Type I is the most durable glass, and Type NP is the least durable glass. Test methods and specification limits for determining the chemical resistance of glass can be found in the current revision of the U. S. Pharmacopoeia, section <661> Containers. USP glass type can be used as a general guide for container selection; however, the applicability of a particular container to an end use cannot be determined only by USP type. General criteria have developed over the years to assist with the selection of glass containers for specific end uses. These guidelines are only recommenda-tions. It is the product manufacturer’s responsibility to ensure that the glass container is suitable for the application and/or contained product.

Glasses in the USP Type I classification are borosilicate glasses with superior chemical resistance. This class of glasses represents the least reactive glass containers available. Typically, these glasses can be used for most applications, including packaging for parenteral and non-parenteral products. Type I glass may be used to package acidic, neutral and alkaline products. Water for injection, un-buffered products, chemicals, sensitive lab samples, and those requiring sterilization are commonly packaged in Type I borosilicate glass. Type I glasses can be subject to chemical attack under certain conditions, so container selection must be made carefully for very low and very high pH applications. Most glass laboratory apparatus are Type I borosilicate glass.

Even though Type I glass has the highest chemical durability, there still may be some sensitivity with certain packaged products. For applications where standard Type I glasses do not provide sufficient protection against alkali extraction and pH shifting, internal surface treatment can be used to further improve the chemical durability of the container. This surface enhancement may become especially important for pH sensitive products packaged in small containers because smaller containers have a higher surface area to volume ratio. See the USP Type II description for an explanation of the internal surface treatment process. It should be noted that the USP does not place any additional durability requirements on surface treated Type I glass.

USP Type II glass is soda-lime glass that has been treated with sulfur compounds to de-alkalize the interior surface of the container. This treatment results in a container with high chemical resistance because alkali is removed from the glass surface prior to use. The amount of ions available to leach into the product is reduced; thus, the container durability is increased. Extraction salts will be present on the interior surface of new sulfur treated containers, and the containers may require washing prior to use. Type II glass is less chemically durable than Type I glass, but is more chemically durable than Type III glass. It can be used for acidic and neutral parenteral preparations that remain below pH 7 during their shelf life.

USP Type III is a soda-lime glass with moderate chemical resistance. It is typically acceptable for packaging dry powders that will be dissolved into solutions or buffers that are insensitive to alkali.

Type III glass may not be suitable for autoclaved products because the autoclaving process will accelerate the glass corrosion reaction. Dry heat sterilization processes are typically not a problem for Type III containers.

USP Type NP is general-purpose soda-lime glass. It is used for non-parenteral applications where chemical durability and thermal shock are not factors. These containers are frequently used for capsules, tablets and topical products. Type III glass is also suitable for all Type NP applica-tions.

It is important to consider filling and processing steps when choosing a container. Both mechanical and thermal stresses are important factors. For a given thermal expansion range, a typical tubing vial with thin, uniform walls will withstand thermal shock better than a molded glass container.

The physical design of the container will play a part in the amount of thermal and mechanical shock resistance it exhibits. It is frequently necessary to make a compromise between high resistance to mechanical shock and high resistance to thermal shock.

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Glass

Symbol used to indicate interchangeable joint, stoppers and stopcocks that comply with the requirements of ASTM E676-02 (taken from commercial standard CS21).

Symbol used to indicate a spherical joint that complies with the requirements of ASTM E677-94 (taken from commercial standard CS21-58).

GPI refers to the Glass Packaging Institute, which is responsible for establishing and issuing uniform voluntary standards regarding the types and finishes produced by American glass manufacturers. When a container finish is designated as 20-400, it means that the diameter across the outside of the threads is approximately 20 mm. (See “T” dimension on illustration.) The 400 designates a specific style of thread. Tables 6a and 6b show average dimensions for comparison and to aid in sizing. The actual dimensions vary slightly and are different from tubing based containers and molded containers.

Light sensitive products must be packaged in amber glass. Amber glass is formulated to absorb light in the Ultra Violet region of the electromagnetic spectrum. Test methods and specification limits for light protection can be found in the current revision of the U. S. Pharmacopoeia, section <661> Containers.

If a product is sensitive to the presence of particular ions, the composition of the glass container should be considered. For example, products that contain sulfate salts may experience the formation of precipitates if packaged in glass with barium or calcium in the formulation. In this example, it would be desirable to avoid glasses that contain barium and calcium. A second example is pre-cleaned containers for environmental sampling. Even though the containers are clean, the chemical durability characteristics of the glass have not been altered. Thus, it would not be feasible to test the samples for low levels of sodium, because the sample will extract sodium from the container’s surface.

24/40 Inner Joint 24/40 Outer Joint 35/25 Ball Joint 35/25 Socket Joint

JS

TS

TS TS JS JS

Container size and physical design. Narrow mouth vs. wide mouth, tall vs. short, etc.

Color. Is light sensitivity an issue? Is amber glass needed?

Shelf life. How long are you planning to store a sample or product in the container?

Method of fabrication. Molded or tubing based?

Processes the container will undergo. Storage conditions (freezing or heat); washing, sterilization; method of sealing; humidity; hot or cold filling; de-pyrogenation.

Storage after filling. Time (shelf life needed); heat, cold, moisture; shipping conditions; light exposure.

Product composition. Dry powder; pH; concentration of ions; physico-chemical properties.

Closure type. Wide mouth vs. narrow mouth; septa lined open top cap; closed cap; liner material; sealing needed; threaded cap or crimp seal.

1.

2.

3.

4.

5.

6.

7.

8.

The minimum opening dimension "I" of a glass container can be found if the containers thread finish is known. If the thread size of the container is 38-400, the "I" can be determined from either Table 6a or 6b by looking down the “T” Dimension column (far left) until you find the number 38. Follow this row to the right, until you come to the “I” min. column that is listed under the number 400. This number is the minimum opening of the container. The opening can be larger, but it should not be smaller.

176Glass



"T” 400 410 415 425 430Dim (mm) “H” “I” min “H” “I” min “H” “I” min “H” “I” min “H” “I” min8 — — — — — — 0.257 0.114 — —10 — — — — — — 0.270 0.148 — —13 — — — — 0.442 0.218 0.295 0.218 — —15 — — — — 0.547 0.258 0.295 0.258 — —18 0.356 0.325 0.513 0.325 0.607 0.325 — — 0.604 0.27020 0.356 0.404 0.544 0.404 0.732 0.404 — — 0.604 0.31222 0.356 0.483 0.575 0.483 0.827 0.483 — — 0.604 0.41024 0.385 0.516 0.636 0.516 0.947 0.516 — — 0.647 0.46028 0.385 0.640 0.698 0.640 1.072 0.640 — — 0.724 0.52530 0.388 0.653 — — — — — — 0.760 0.56833 0.388 0.791 — — — — — — 0.775 0.70335 0.388 0.875 — — — — — — — —38 0.388 0.987 — — — — — — 0.946 0.82840 0.388 1.091 — — — — — — — —43 0.388 1.165 — — — — — — — —45 0.388 1.251 — — — — — — — —48 0.388 1.381 — — — — — — — —51 0.393 1.479 — — — — — — — —53 0.393 1.578 — — — — — — — —58 0.393 1.735 — — — — — — — —60 0.393 1.853 — — — — — — — —63 0.393 1.972 — — — — — — — —66 0.393 2.090 — — — — — — — —70 0.393 2.247 — — — — — — — —75 0.393 2.424 — — — — — — — —77 0.472 2.546 — — — — — — — —83 0.472 2.753 — — — — — — — —89 0.520 2.918 — — — — — — — —100 0.582 3.344 — — — — — — — —110 0.582 3.737 — — — — — — — —120 0.670 4.131 — — — — — — — —


"T” 400 410 415 425 430Dim (mm) “H” “I” min “H” “I” min “H” “I” min “H” “I” min “H” “I” min8 - - - - - - 6.53 2.90 - -10 - - - - - - 6.86 3.76 - -13 - - - - 11.23 5.54 7.49 5.54 - -15 - - - - 13.89 6.55 7.49 6.55 - -18 9.04 8.26 13.03 8.26 15.42 8.26 - - 15.34 6.8620 9.04 10.26 13.82 10.26 18.59 10.26 - - 15.34 7.9222 9.04 12.27 14.61 12.27 21.01 12.27 - - 15.34 10.4124 9.78 13.11 16.15 13.11 24.05 13.11 - - 16.43 11.6828 9.78 16.26 17.73 16.26 27.23 16.26 - - 18.39 13.3430 9.86 16.59 - - - - - - 19.30 14.4333 9.86 20.09 - - - - - - 19.69 17.8635 9.86 22.23 - - - - - - - -38 9.86 25.07 - - - - - - 24.03 21.0340 9.86 27.71 - - - - - - - -43 9.86 29.59 - - - - - - - -45 9.86 31.78 - - - - - - - -48 9.86 35.08 - - - - - - - -51 9.98 37.57 - - - - - - - -53 9.98 40.08 - - - - - - - -58 9.98 44.07 - - - - - - - -60 9.98 47.07 - - - - - - - -63 9.98 50.09 - - - - - - - -66 9.98 53.09 - - - - - - - -70 9.98 57.07 - - - - - - - -75 9.98 61.57 - - - - - - - -77 11.99 64.67 - - - - - - - -83 11.99 69.93 - - - - - - - -89 13.21 74.12 - - - - - - - -100 14.78 84.94 - - - - - - - -110 14.78 94.92 - - - - - - - -120 17.02 104.93 - - - - - - - -

177


Glass

Although most types of glass vials and containers are sterilizable by either steam or dry heat, some compositions perform better than others. Most Type I borosilicate glass is suitable, when proper techniques are followed, for sterilization and de-pyrogenation. Type III is not recommended for repeated steam sterilization, although this may be appropriate on a single use basis. Recommended autoclave cycles are 121°C @ 15psig for 20 minutes. Caps should be left loose on the containers. Proper care must be given when venting back to atmosphere or there may be damage to the containers.

Dry heat sterilization can be achieved at a temperature of 160°C for 2 to 3 hours, but glass containers are capable of withstanding sterilization temperatures up to 500°C without noticeable degradation of the glass. Repeated dry heat sterilization of containers containing a fair amount of moisture may be susceptible to glass flaking. Inversion of the container and good ventilation would prevent this from occurring. Inspect glass containers for chips, cracks, and scratches before each use and discard if damage is evident, as breakage may occur during sterilization if used. Glass containers may also be sterilized using gas or chemicals. Ethylene oxide (EtO), formaldehyde, or peroxide gas is generally used when heat and pressure cannot be used due to material limitations. Chemical disinfectants normally used are quaternary ammonium compounds, iodophors, formalin, benzalkonium chloride, and ethanol.

Glass containers may also be sterilized using irradiation, however, the process changes the color of the glass, which may not be acceptable for most applications. There is glass tubing available, which will not change color when irradiated. This would be available for those interested in large quantity orders of tubing vials only.

Modern high-speed mold production of glass containers requires the use of release agents or coatings on the metal mold equipment to prevent sticking and malformation of the bottles in the molding process. A variety of coatings or lubricants are used to provide optimum viscosity and function according to the particular needs of the individual piece of process equipment as well as service conditions.

The coatings are compounded from colloidal graphite and sulfur suspended in hydrocarbon oils and waxes with small amounts of modifiers such as calcium soaps and greases. This “mold dope” is replenished through periodic swabbing of the mold equipment. The hot forming temperature and the subsequent lehr annealing (1000 – 1100°F) process will burn off the volatile sulfur and organic oils and waxes. Portions of carbon can remain since the major component (graphite) is very slowly decomposed and oxidized in the process. Quality control in manufacturing employs a number of devises (both automatic and manual) to eliminate the small percentage of product that has excess graphite spots.

Because the conditions under which glassware is used vary widely, there is no guarantee against breakage. Always exercise care to protect personnel and property when using any vessel with vacuum or pressure. Never subject glassware showing visible signs of damage (chipped, cracked or scratched) to pressure or vacuum.

When glass containers are formed, the surface of the glass is enriched in alkali. The annealing process further enhances this effect. This phenomena is usually of no practical consequence and goes unnoticed, but in certain circumstances, it interferes with further processing of the container. As glass is exposed to the atmosphere, a complex reaction occurs on the surface between the alkali on the glass and gasses in the air. These reactions are commonly known as weathering. The reaction produces salts, which can absorb water from the air. It is these salts that are the source of surface related decorating problems. Weathering salts are composed of a mixture of various hydrates of sodium carbonate and sulfate along with minor amounts of similar calcium salts. Weathering is a normal condition and such salts are always found on glass surfaces as they are exposed to the atmosphere. The quantity and crystal appearance will vary depending upon time, humidity and temperatures of storage. These salts are easily removed by water rinsing.

All glasses weather, but some are more resistant than others. Borosilicate glasses are most resistant, followed by durable soda-lime, and common soda lime. Since glass containers can be decorated in a myriad of ways, the weathering of glass must be considered in selecting a method that is effective and trouble free. Table 5 is a rough guide to decorating and labeling of glasses.

The surface treatments used to remove weather salts or remove the alkali that cause weathering are somewhat limited. Since the salts are water soluble, a simple wipe with a wet cloth or washing prior to decoration or pressure sensitive labeling is effective in most cases.

Heat and humidity cycling or storing glass in a confined space promotes weathering. Keeping the glass under constant low humidity is effective in slowing weathering as it keeps the surface dry and reduces the salt build-up. Dry heating the container just prior to decorating or pressure sensitive labeling is sometimes successful.

A layer of absorbed moisture on the glass prevents good adhesion of pressure sensitive labels. Water based adhesives, however, would be no problem. Several possible solutions to the problem can be suggested:

1. Simply storing the containers in an area of low humidity for several days may solve the problem.

2. Washing or wiping the ware with warm water. This removes the weathering salts and allows the achievement of a moisture-free surface.

3. Heating the ware will dry the surface and allow good adhesion of the labels. Heating will not remove the salts so the heating must be accomplished shortly before labeling.

The presence of dry salts on the ware will not cause a problem, but the salts can again, rapidly absorb moisture.

(√ = O.K.) Borosilicate Soda-Lime

Rate of Weathering Very slow (months) Slow under low humidity. Rapid under high humidity. (typically weeks)

Pressure Sensitive √ √ Success varies Labels with amount of weathering

Glue Labels √ √

Ceramic Screen √ √

Organic Screen √ √

Gold √ √ If not severely weathered

178Glass


The suggested conditions for steam sterilization are 121°C (250°F) @ 15 psig for 20 minutes. Portions of the coating may absorb a small amount of water vapor and appear cloudy after autoclaving, however, the cloudiness will disappear as the coating dries. To speed clearing, glassware can be dried in an oven at 49 – 66°C (120 – 150°F). Autoclaving effects on the coating will vary slightly due to equipment, container size and configuration, procedure, and frequency of procedure. It is recommended that containers not be autoclaved touching each other to avoid possible sticking problems. Also, it is recommended that the autoclave pressure be allowed to return to zero before removing glassware. A sudden release of pressure may cause the coating to separate from the glass and produce air pockets under the coating.

Evaluation of a sample is the best way to determine if the safety coating will work for your application.

For after-use disposal, PVC safety coated containers create a unique situation in that they are a composite package of glass and plastic. Depending on the application, there are four ways to handle the disposal of coated containers:

In the laboratory or industrial setting, coated containers can be washed, dried, and reused, perhaps for the collection of hazardous waste in the laboratory.

For consumer pharmaceutical and cosmetic applications, coated glass containers should be able to go into residential glass recycling collection. Coated glass makes up such a small percentage of total glass collected that it should not present any recycling problems (variations in state and county recycling programs make it difficult to generalize).

For large quantity industrial or laboratory applications, recycling coated glass containers, as a whole, can create two problems: the grinding of the coated glass into cullet could be difficult, and the PVC in the glass furnace might create organic chlorides in the glass mixture which would affect the final pH of the glass. Also, if a hazardous material was packaged in the containers, many recyclers do not want to accept the glass.

The plastic coating can be cut and peeled from the container and the glass and the plastic jacket recycled, but for safety reasons this is not recommended. Both glass and PVC are recyclable materials. Stripped coatings are recycled into garden hoses and floor mats.

A plastisol coating was developed to contain glass fragments and allow for a controlled release of the contents in the event of container breakage. The coating:

Adds impact, thermal shock, and slip resistance

Contains glass - prevents flying fragments and cuts

Contains contents - reduces risk of chemical exposure and inhalation. Allows time for proper disposal.

The coating material is plastisol, which is a dispersion of a fine particle size PVC resin (polyvinyl chloride) in a plasticizer where stabilizers, fillers, modifiers, colorants, and other compounding ingredients may be added. When the plastisol is heated, the suspended PVC particles begin to swell and absorb the surrounding liquid plasticizer. When the temperature is increased to over 300°F, fusion of the particles occurs and the particles coalesce into a homogeneous mass. The coating process is a heat-and-time related process that determines coating weight and thickness and is controlled by machine line speeds and oven temperatures. The more heat, the heavier the coating, and the slower the line, the heavier the coating.

Non-autoclavable coated containers can be used successfully at 121°C (250°F) and below. Do not use above 300°F or over direct heat or flame. The coating is not dry heat sterlizable. Coating will yellow and burn with high heat exposure but will continue to protect until black.

Labeling of plastisol coated glass containers has always been somewhat of a problem. It is important to select a face stock and adhesive combination with the proper performance characteristics for the intended product and application. It is recommended that prior to the selection of any adhesive, the customer contact the adhesive manufacturer or supplier and discuss the application requirements.

For on-line and pressure sensitive labeling of plastisol coated glassware, an acrylic based adhesive with low rubber and vinyl content is recommended. Other label adhesives will usually extract the plasticizer from the coating, become soft, bleed through the label, and eventually lose adhesion. Acrylics block the plasticizer extraction and allow the initial adhesion to remain undisturbed. There are, however, many variations of acrylic based adhesives and some are more effective than others. Adhesives are usually formulations of several chemicals that are combined in a variety of ratios and available in many forms. It is for these reasons, that accelerated age testing is advisable.

When selecting an adhesive for a specific application, consideration should be given to the necessary bond strength and duration, moisture, UV, heat, and solvent resistance. There is no substitute for proper testing of the proposed materials under actual usage conditions. The final decision should be made by the customer to choose the label/adhesive combination that meets the requirements of the specific use.

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Coated containers can be crushed and safely landfilled. The plastic jacket is made of PVC material, which is very chemically stable and does not leach out harmful chemicals into groundwater. In fact, PVC is often used to make liners for landfill sites.

Coated containers can be incinerated. PVC is often blamed for the release of toxic dioxins and hydrochloric acid (HCL). However, research has shown that dioxins and hydrochloric acid are generated no matter what amount of PVC is present in the waste. Incinerator operating conditions and temperatures determine the amounts produced. Hydrochloric acid, which can cause acid rain, can be controlled in a modern incinerator equipped with a proper scrubber.

The safety coating was developed to contain the glass fragments and allow for a controlled release of the contents in the event of container breakage. In addition, the coating adds impact, thermal shock, and slip resistance, prevents flying fragments and cuts, and reduces risk of chemi-cal exposure and inhalation. Few, if any, alternative-coating materials have been found that perform as well or better than PVC plastisol.

Most household glass containers are manufactured from soda-lime glass. All of our soda-lime glass may be recycled in the same manner after performing any necessary decontamination procedures. Borosilicate glass must not be mixed and recycled with soda-lime glass.

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Glass

Handle glassware carefully. Most damage to glassware occurs during cleaning.

Glassware should be washed as soon as possible after use to avoid caking of residue. It is important not to let soiled glassware dry out. If immediate cleaning is not possible, the glassware should be put to soak in water. Use of a cleaning agent is recommended. Glassware should not to be cleaned with harsh or abrasive cleaners. It is recommended that a mild detergent or non-abrasive cleaner be used. Hard utensils, wire brushes or bottle brushes with wire cores, should not be used for cleaning. It is recommended that a sponge brush that is soft and flexible be used. Scratched glassware is prone to breakage during freezing or heating.

After washing, the glassware should be rinsed with tap water to remove any cleaning agent residue. After the tap water rinse, the glassware should be rinsed with distilled or deionized water. Dry the glassware inverted on racks or pegboards. Inspect the glassware for chips, cracks, and scratches on the inside and outside. Do not use glassware with visible signs of damage.

180Plastics


Cavity that receives the Preform, which will be blown into the desired shape.

Used in Extrusion Blow Molding. Hollow tube that pierces Preform and introduces air to blow Preform into shape of Blow Mold.

That part of the mold, which contains the reverse image of the product being formed.

Flow channel for heat-softened polymer, which goes from the Plastifier to the mold cavities. Polymer in the flow channel is cooled with shaped parts in cavities and is later removed, reground, and reused.

That part of a mold that allows the internal shaping of a product such as the internal threads of a cap.

Used in Injection Blow and Injection Stretch Blow Molding. Used in conjunction with a Preform Mold to manufacture a Preform. The Preform is formed around the Core Rod creating a hollow tube, which will then be transferred to a Blow Mold where air will be introduced forcing the Preform to take the shape of the Blow Mold cavity.

A molding process whereby heat-softened polymer is forced into the shape of a hollow tube. While still soft, a mold closes around the tube, pinching the top and bottom of the tube closed. A Blow Pin is introduced, and air is forced through the pin forcing the tube to take the shape of the Blow Mold cavity.

A method of rendering inert thermoplastic objects receptive to inks, lacquers, paints, adhesives, etc. in which the object is bathed in an open flame to promote oxidation of the surface of the article. Polyolefins (HDPE, LDPE, PP, etc) are primarily those polymers that are flame treated.

Extra plastic attached to molded ware along the parting line, which must be removed before the part can be considered finished.

Used in Injection, Injection Blow, and Injection Stretch Blow Molding. The orifice through which the heat-softened polymer enters the cavity.

Flow channel for heat-softened polymer, which goes from the Plastifier to the mold cavities. Polymer in the flow channel is kept softened so there is no runner material to grind up and reuse.

Conical feed reservoir into which polymer pellets are loaded. These pellets then fall into a heated barrel (Plastifier), sometimes through a metering device.

A molding process in which heat-softened polymer is injected from a Plastifier into a mold cavity creating a Preform, which is then transferred to a Blow Mold where air is blown into the Preform, forcing it to take the shape of the Blow Mold cavity.

A molding process whereby a heat-softened polymer is injected from a Plastifier into a relatively cool cavity, which gives the article the desired shape.

A molding process whereby Preforms are introduced into a cavity, stretched axially by a Stretch Rod, and then blown circumferentially to the shape of the Blow Mold cavity.

The amount, in grams, of a thermoplastic resin, which can be forced through a 0.0825 inch orifice when subjected to 2160 gms. force in 10 minutes at 190°C.

Contains the cavity or cavities of a desired part in which a heat-softened polymer is shaped.

A line formed at the point of contact of the Mold halves.

Part of the mold assembly, which forms the neck and finish of a container.

Hollow cored orifice that is screwed into the extrusion end of the Plastifier. The nozzle is designed to form a seal under pressure between the Plastifier and the Mold or Runner system. The front end of a nozzle may be either flat or spherical in shape.

Assembly whereby polymer pellets are fed from a Hopper into a barrel where they drop onto a turning screw which forces the pellets forward. Heater bands wrapped around the barrel melt the pellets as they are forced forward along the inside of the barrel. The molten polymer is then forced out the end of the barrel through the Nozzle.

Used in Blow Molding processes. Heat-softened polymer is formed into a shape similar to a thick test tube with neck threads. This tube is subsequently inflated while inside a Blow Mold to create the shape of the desired article.

A thermoplastic from a processor’s own production that has been reground or re-pelletized after having been previously processed by molding.

A lubricant that facilitates molding.

Used in Injection Stretch Blow Molding. A rod that is introduced into the Preform to stretch it in an axial direction prior to the Preform being blown into the shape of the cavity.

Material that will repeatedly soften when heated and harden when cooled.

181


Plastics

Listed below are the primary resins used in the manufacture of our products. Following are some of the characteristics and features of these resins.

Family of highly transparent plastic, which features high moisture barrier and excellent chemical resistance properties. Poor gas barrier properties. Sterilizable via EtO, radiation and certain grades can be steam autoclaved. SPI resin identification code number - (7).

Flexible but more rigid than LDPE. Natural color is milky white, semi-translucent depending on density. Good impact strength and stress crack resistance. Good chemical resistance. Good vapor barrier but poor gas barrier. Sterilizable via EtO or gamma radiation. SPI resin identification code number - (2).

Very flexible, natural milky color, translucent with high impact strength. Excellent for mild and strong buffers, good chemical resistance. Good water vapor and alcohol barrier properties. Poor gas barrier, sterilizable with EtO or gamma radiation. Good stress crack and impact resistance. SPI resin identification code number - (4).

Good chemical resistance, clear color, resistant to water, weak acids and bases at room temperature. Can be sterilized by EtO and autoclaving, at temperatures up to 180°C. SPI resin identification code number - (7).

Rigid and strong, Excellent clarity. High impact strength. Poor barrier properties. SPI resin identification code number - (7).

COC* HDPE LDPE PET PP PS PVC TFE

Max. Temp °C 140 120 80 60 135 70 70 240Transparency transp transl transl transp transl transp transl opaqSterilization* * Autoclave yes no no no yes no no yes Gas yes yes yes yes yes yes yes yes Dry Heat no no no no no no no yes Radiation yes yes yes yes no yes no no Disinfectants yes yes yes yes yes no yes yesDensity g/cm3 1.02 0.95 0.92 1.33 0.90 1.06 1.34 2.15Flexibility rigid semi flex semi rigid rigid rigid rigidBrittleness Temp °C +20 -100 -100 -10 0 +20 -30 -110Tensile Strength, psi 9600 4000 2000 8000 5000 6000 5000 4000* Topas® 6015 resin**Depends on thickness and relates to containers and caps. Because there are many grades of resins and processing methods, the above information should be used as a general guideline only.

COC* HDPE LDPE PET PP PS PVC TFE

N2 See Note 1 — 42 180 0.8 44 50 2 —

O2 See Note 1 180 150 500 5 90-140 185-485 4 —

CO2 See Note 1 — 580 2700 15 650 1160 4 —

Moisture See Note 2 0.2 0.3 1.3 2.0-4.0 0.3-0.7 8.5 1.0-5.0 —Note 1: Units are cc x mil /100 in2 x day x atm @ 25°CNote 2: Units are g x mil /100 in2 x day @ 38°C, 50 - 90% RH

Semi-rigid to rigid depending on wall thickness. Natural color - clear and transparent. Good alcohol and solvent barrier; good gas and fair moisture barrier. Good to fair chemical barrier; not good for strong acids or bases. Good moldability. Sterilizable through EtO and gamma radiation. Good stress crack and impact resistance at room temperature and above. SPI resin identification code number - (1).

Rigid, solid, durable in container or cap forms. Opaque, natural gray-ish yellow in natural form. Excellent stress crack and impact resistance. Excellent moisture barrier, good oil and alcohol barrier, poor gas barrier properties. Good chemical resistance. Sterilizable with EtO or autoclaving. SPI resin identification code number - (5).

PS is a transparent, rigid and glass-like polymer. Good resistance to inorganic chemicals. Light and heat stable, biologically inert and non-toxic. Poor impact and stress crack resistance, poor barrier properties. EtO or Gamma sterilizable. SPI resin identification code number - (6).

Flexible to rigid. Good for coatings; fair water and good oxygen barrier. Transparent to yellowish color in natural state. Good chemical resistance. Sterilizable by EtO. Good impact and some stress crack resistance. Poor recycling due to chloride residues. SPI resin identification code number - (3).

Tetraflouroethylene, fluorinated ethylene propylene. All Teflon® plastics feature opaque characteristics, excellent chemical resistance, good heat stability and thermal shock resistance. All are autoclavable, heat, and gas sterilizable. SPI resin identification code number - (7).

182Plastics


P lastic containers have been developed for a variety of applications across many different industries over the years. There are many different types of Polymers used in the creation of these containers to help fill the demands for the

various applications. Polymers offer a variety of properties, each having different levels of importance with different users depending on the application. Some users may have flexibility within their product formula or filling process and therefore focus on economical containers while others may need containers that are stronger, autoclavable, transparent, sterilized, etc., therefore requiring more specifications. Wheaton Science Products can help with polymer selection through comprehension of the customer’s product, goals, and adaptability. Several questions should be posed to gather this understanding. Some examples include:

What is the container size and physical design. Narrow mouth vs. wide mouth, tall vs. short, etc.?

Must the package be transparent, translucent, opaque or colored for either marketing or light protection?

Are there specific shipment and storage conditions such as refrigeration, freezing, exclusion of light, etc.?

Are there governmental regulations pertaining to the product?

How is the product going to be dispensed by the user?

Have any tests been run in plastic? Was it unsuccessful and why? What type of plastics?

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Many things govern polymer suitability for package use. These include:

Permeation/BarrierSorption CharacteristicsChemical ResistanceStress Crack ResistanceRigidity/FlexibilityImpact ResistanceSterilizabilityRecyclabilityTemperature Resistance

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183


Plastics

Plastic products and containers are considered to be biologically inert. For example polyethylenes, Teflons, polypropylene, polystyrene, and polycarbonate are considered to be non-toxic to cell cultures. Distilled water for preparing culture media can be collected and stored in polyethylene containers.

When a container finish is designated as 24-410, it means that the diameter across the outside of the threads is approximately 24 mm. (See “T” dimension on illustration.) The 410 designates a specific style of thread. Tables 10a and 10b show average dimensions for comparison and to aid in sizing. The actual dimensions may vary slightly.

The minimum opening dimension (I) of a plastic container can be found if the container’s thread finish is known. If the

thread size of the container is 38-400, the (I) can be determined from either Table 10a or 10b by looking down the “T” Dimension column (far left) until you find the number 38. Follow this row to the right, until you come to the “I” min. column that is listed under the number 400. This number is the minimum opening of the container. The opening can be larger, but it should not be smaller.

Requirement COC PC PE PET PP PS PVC

Light weight 3 6 2 5 1 3 6

Clarity 1 1 3 1 2 1 1

Toughness 6 3 1 2 3 9 8

Water adsorption 1 6 2 3 2 4 2

Water vapor permeability 1 7 2 5 2 5 4

CO2 permeability 6 1 6 2 5 9 3

O2 permeability 7 8 7 2 6 8 2

Resistance: Acids 2 4 2 4 2 4 2

Resistance: Alkalis 2 7 2 2 2 2 2

Resistance: Oils 5 4 4 2 3 4 2

Resistance: Solvents 3 6 3 2 3 6 4

Resistance: High Humidity 1 6 1 1 1 1 1

Resistance: Sunlight 4 4 4 1 4 5 5

Resistance: Heat (hot fill) 1 1 3 1 2 5 1

Resistance: Cold 5 1 1 2 4 5 9

This chart is a generalization to aid in selection; there are many forms, thicknesses and various copolymers and additives available. The lower the number, the better the property.

1=Excellent; 9=Poor.

184Plastics



"T” 400 410 415 425Dim (mm) “H” “I” min “H” “I” min “H” “I” min “H” “I” min

13 — — — — 0.452 0.218 0.310 0.21815 — — — — 0.557 0.258 0.310 0.25818 0.371 0.325 0.523 0.325 0.617 0.325 — —20 0.371 0.404 0.554 0.404 0.742 0.404 — —22 0.371 0.483 0.585 0.483 0.837 0.483 — —24 0.400 0.516 0.646 0.516 0.957 0.516 — —28 0.400 0.614 0.708 0.614 1.082 0.614 — —30 0.403 0.653 — — — — — —33 0.403 0.791 — — — — — —35 0.403 0.875 — — — — — —38 0.403 0.987 — — — — — —40 0.403 1.091 — — — — — —43 0.403 1.165 — — — — — —45 0.403 1.251 — — — — — —48 0.403 1.381 — — — — — —51 0.408 1.479 — — — — — —53 0.408 1.578 — — — — — —58 0.408 1.735 — — — — — —60 0.408 1.853 — — — — — —63 0.408 1.972 — — — — — —66 0.408 2.090 — — — — — —70 0.408 2.247 — — — — — —75 0.408 2.424 — — — — — —77 0.487 2.546 — — — — — —83 0.487 2.753 — — — — — —89 0.535 2.918 — — — — — —100 0.597 3.344 — — — — — —110 0.597 3.737 — — — — — —120 0.685 4.131 — — — — — —


“T” 400 410 415 425Dim (mm) “H” “I” min “H” “I” min “H” “I” min “H” “I” min

13 — — — — 11.48 5.54 7.87 5.5415 — — — — 14.15 6.55 7.87 6.5518 9.42 8.25 13.28 8.25 15.67 8.25 — —20 9.42 10.26 14.07 10.26 18.85 10.26 — —22 9.42 12.27 14.86 12.27 21.26 12.27 — —24 10.16 13.11 16.41 13.11 24.31 13.11 — —28 10.16 15.59 17.98 15.59 27.48 15.59 — —30 10.24 16.59 — — — — — —33 10.24 20.09 — — — — — —35 10.24 22.22 — — — — — —38 10.24 25.07 — — — — — —40 10.24 27.71 — — — — — —43 10.24 29.59 — — — — — —45 10.24 31.77 — — — — — —48 10.24 35.08 — — — — — —51 10.36 37.57 — — — — — —53 10.36 40.08 — — — — — —58 10.36 44.07 — — — — — —60 10.36 47.07 — — — — — —63 10.36 50.09 — — — — — —66 10.36 53.09 — — — — — —70 10.36 57.07 — — — — — —75 10.36 61.57 — — — — — —77 12.37 64.67 — — — — — —83 12.37 69.93 — — — — — —89 13.59 74.12 — — — — — —100 15.16 84.94 — — — — — —110 15.16 94.92 — — — — — —120 17.40 104.93 — — — — — —

There are a variety of plastic materials and methods by which these plastic materials can be sterilized. However, not all plastics can be sterilized by every method. An understanding of sterilization methods, problems that can occur and terms associated with sterilization is helpful in determining plastic and plastic ware capability and performance. The following is presented to assist in gaining that understanding.

While temperature and time used to melt thermoplastics kills microorganisms, manufactured ware will not remain sterile unless it is made and maintained in a sterile environment. Plastic ware is not “sterile as manufactured“ since:

ware is not blown with sterile airware may be exposed to non-sterile conditions immediately after

manufactureware may contact non-sterile atmosphere, bags, boxes, personnel,

etc. during packing after ware manufacture or during unpacking at the filling location.

low particulate does not mean sterile

Producing ware under a shroud and using “particulate-free” or “low particulate” clean room bags does not result in sterile ware. These steps only reduce particulate in and on the ware to a lower level than would be present if ware were produced in an “unshrouded” production situation. In the future, molding may be performed in clean rooms and sterile conditions maintained after ware manufacture, however, until that time, ware cannot be represented as being sterile as molded. Until then, a secondary sterilization process must be performed.

This is the number of microorganisms (bacteria, virus, fungi, etc.) present. Microbiologists can test for these. When sterilizing ware, it is important to reduce or eliminate the bioburden to a safe level.

A pyrogen, which means fever causing, is a remnant of bacteria that carries chemicals called endotoxins. Endotoxins can cause a fever if injected into a mammal. Detecting a fever in a rabbit after injecting it with an extract detects the presence of pyrogens.

Something may be sterile, but still have pyrogens on it. Glass can be sterilized and de-pyrogenated at the same time. Exposure to high temperature (600°F or higher) will kill microorganisms AND burn up endotoxins. The higher the temperature, the shorter the exposure time needed for de-pyrogenation. Most plastic ware is incapable of being exposed to these high temperatures. Therefore, plastic ware may be sterilized but, if it needs to be de-pyrogenated, it is usually washed with pyrogen free water.

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Sterilization techniques are designed to kill microorganisms. There are varieties of sterilization methods, however the three basic approaches used to sterilize plastic ware are:

Ethylene Oxide (EtO)Steam AutoclaveRadiation (gamma radiation, electron beam radiation)

Tests should always be run on plastic ware to determine suitability for a given sterilization method.

Ethylene oxide (EtO) is a toxic, cancer causing gas. Technology and worker protection legislation allow continued EtO use. Most plastic can be EtO sterilized. EtO must contact the surfaces to be sterilized. There are several ways EtO sterilization can be accomplished.

Empty ware in an open bag or ware in a sealed bag with a “breather” window, is placed in a chamber. Air is evacuated and moisture introduced (dry microorganisms are resistant to EtO sterilization).

Pure EtO is flooded into the chamber. Chamber internal pressure is kept lower than external pressure to ensure gas will not leak. Exposure time varies dependent on ware and bioburden. After exposure, the chamber is purged with filtered sterile air to eliminate residual EtO.

Since it is safer than pure EtO, a 10-15% mixture of EtO with inert gas is used. Empty ware in an open bag or ware in a sealed bag with a “breather window” is placed in a chamber. Air is evacuated, and moisture is introduced (dry microorganisms are resistant to EtO sterilization). Dilute EtO is flooded into the chamber and the chamber's temperature increased up to 60°C (140°F). Exposure time of 4 to 24 hours varies dependent on ware, bioburden, and sterilization parameters. After exposure, the chamber is purged with filtered sterile air to eliminate residual EtO.

Most plastic ware is capable of being EtO sterilized. However, zinc stearate process aid, used in injection blow molding, can cause precipitants (particulate) to form in liquid products packaged in EtO sterilized ware.

Therefore, only special LDPE grades and colorants that do not require zinc stearate for injection blow molded ware should be used with EtO sterilization processes. Additionally, tests should always be run on plastic ware to determine suitability for a given sterilization method.

Autoclaving can sterilize empty OR filled, sealed ware. The effect of temperature AND moisture kills microorganisms. Autoclaving involves exposing ware for a time to steam. The autoclave acts like a pressure cooker allowing steam temperature to get above the boiling point of water (100°C=212°F). Typically, autoclaving is done at 15 psi (pounds per square inch) steam being at 121°C (250°F).

Empty ware must withstand autoclaving temperature for the exposure time. If it does not, parts will distort. Of the common plastics, polypropylene (PP) and polycarbonate (PC) have enough

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185


Plastics

heat resistance to be autoclaved. Generally, PP homopolymer is slightly more heat resistant than PP copolymer. Also, there is a grade of a new transparent plastic material identified as a cyclic olefin copolymer (COC) that is capable of withstanding steam autoclave sterilization.

If empty ware becomes distorted due to autoclave sterilization, it may be due to:

High stresses molded into the ware during manufactureUnusual hot spots in the autoclaveUse of the wrong plastic


Autoclave sterilization of filled, sealed ware, is also known as “Terminal Sterilization”. Many companies prefer terminal sterilization IF their product can withstand the rigors. Autoclave temperature must be minimally 121°C (250°F). Of the common plastics, polypropylene (PP) and polycarbonate (PC) have enough heat resistance to be autoclaved. Also, there is a grade of a new transparent plastic material identified as a cyclic olefin copolymer (COC) that is capable of withstanding steam autoclave sterilization. However, autoclaving filled, sealed plastic ware is tricky. Tempera ture and pressure in the autoclave must be controlled and balanced with temperature and pressure being generated in the filled, sealed ware during autoclave heat up AND cool down. If not, ware could be crushed or ballooned. Special autoclaves are sold to enable this temperature/pressure balancing act.

If filled, sealed containers become distorted during autoclave sterilization, it may be due to:

Improper balancing of temperature/pressure upon heating or cooling

High stresses molded into ware at the time of manufactureUnusual hot spots within the autoclave chamberUse of the wrong plastic


Polypropylene (PP) closures should be capable of withstanding steam autoclave sterilization. However, autoclaving may cause blooming of additives in PP. PP homopolymer is more heat resistant than PP copolymer. Linerless closures (closures with specially molded-in sealing features) may or may not be acceptable for autoclaving dependent on many factors (e.g. as application torque, autoclave conditions, closure design, etc.) If a closure is lined, the liner and the adhesive used to affix the liner inside the closure must also be considered. Lastly, PP closures applied to containers present a special case. Closures are designed with tolerances that cause interference between the closure and container. This interference results in stress. Since all thermoplastics become softer as temperature increases, stress may be relieved or closure dimensions may change upon autoclaving. This can result in closure torque reduction or seal loss.

If closures distort or a torque retention problem results, it may be due to:

High stresses molded into ware at the time of manufactureUnusual hot spots within the autoclave chamberUse of the wrong plastic

Due to moisture absorption, pulp liners are NOT anticipated to be acceptable for autoclaving. Tests should always be run on plastic ware and liner/adhesive combinations to determine suitability for a given sterilization method.

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Ware is exposed to ionizing radiation that knocks electrons off atoms it contacts. Ionizing radiation is lethal to microorganisms because of its destructive effect upon the contents of living cells. There are two common sources of ionizing radiation used for sterilization:

Cobalt 60 (gamma radiation) OR

Electron beam or E-beam (high energy electrons)

The amount of radiation from either Cobalt 60 or electron beam is measured in MegaRads (MRads) or KiloGrays (KGy). One MegaRad equals ten KiloGrays. Because gamma sterilization and E-beam both use radiation, packaging materials react similarly in both systems.

A gamma radiation sterilization facility consists of a thick walled concrete maze in a room built around a well filled with water. In the well are a number of pencil-sized steel rods impregnated with radioactive Cobalt. Articles to be sterilized are placed on conveyors that bring them through the concrete maze into the room where the radioactive rods are located. The number of rods raised from the well and the exposure time controls the degree of exposure. After exposure, ware is conveyed from the room via the maze.

A radiation dose sufficient to kill bacteria and spores is about 2.5 MRads. To minimize costs plus attain sterilization, bioburden is de-termined then the minimum dosage plus a safety factor is selected.

Gamma radiation has high penetrating power (about 50 cm or close to 20 inches of the same unit-density material). Thus, many parts can be packed together for sterilization. In this instance, the dosage reaching the center of ware multi-packs is validated. Slightly higher doses occur at the outside edges of multi-packs.

Usually, empty packaging components are sterilized via gamma radiation. Since effects of radiation are cumulative, twice the normal dose is sometimes examined to insure minimal problems.

Listed below are thermoplastic materials that are recognized as capable of being gamma radiation sterilized, although tests should always be run on plastic ware to determine suitability for a given sterilization method:

Low Density Polyethylene

Linear Low Density Polyethylene

High Density Polyethylene (those containing phosphite stabilizers may yellow)

Polyethylene Terephthalate

Polystyrene

Polycarbonate

Nylon

Cyclic Olefin Copolymers (a newly emerging group of polymers)

Polyethylene Naphthalate (a newly emerging group of polymers)

Problems can occur when gamma radiation sterilizing polyvinyl chloride (PVC) or fluoropolymers (Teflon, etc.)

Important Note About Polypropylene Gamma Radiation Sterilization Normal PP grades yellow noticeably and exhibit long term embrittlement when sterilized via gamma radiation techniques. Special radiation resistant PP grades, having special stabilizers, are available for radiation sterilization. Also, if ware is to be colored, then the concentrate carrier should be a radiation resistant grade of PP. PP copolymers are more radiation resistant than PP homopolymers. Tests should always be run on plastic ware to determine suitability for a given sterilization method.

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186Plastics


An E-beam radiation sterilization facility consists of a protective maze built around an E-beam generator. The E-beam generator delivers a high dose of electrons focused in a narrow beam at the items to be sterilized. After exposure, ware is conveyed from the maze.

A radiation dose sufficient to kill bacteria and spores is about 2.5 MRads. To minimize costs and attain sterilization, bioburden is determined and the minimum dosage plus a safety factor is selected.

Electrons from the E-beam generator have limited penetrating power (a 10-MeV E-beam will penetrate only about 5 cm or 2 inches of a unit-density material). Thus, a limited number of parts can be packed together for sterilization. The dosage reaching the center of a ware multi-pack is validated. Higher dosages will occur at the outside edges of ware multi-packs.

Usually, empty packaging components are sterilized via E-beam. Since effects are cumulative, twice the normal dose is sometimes examined to insure minimal problems.

Listed below are thermoplastic materials that are recognized as capable of being electron beam radiation sterilized, although tests should always be run on plastic ware to determine suitability for a given sterilization method:

Low Density Polyethylene

Linear Low Density Polyethylene

High Density Polyethylene (those containing phosphite stabilizers may yellow)

Polyethylene Terephthalate

Polystyrene

Polycarbonate

Nylon

Cyclic Olefin Copolymers (a newly emerging group of polymers)

Polyethylene Naphthalate (a newly emerging group of polymers)

Problems can occur when E-beam sterilizing polyvinyl chloride (PVC) or fluoropolymers (Teflon, etc.)

Normal PP grades yellow noticeably and exhibit long term embrittlement when sterilized via E-Beam. Special PP grades, having special stabilizers, are available for E-beam sterilization. Also, if ware is to be colored, then the concentrate carrier should be a radiation resistant grade of PP. PP copolymers are more radiation resistant than PP homopolymers. Tests should always be run on plastic ware to determine suitability for a given sterilization method.

DISCLAIMER: The data and information contained herein are limited to illustrative examples that are based on limited testing procedures. However, due to variable conditions of use and methods or processing, NO GUARANTEES OR WARRANTIES ARE EXPRESSED OR IMPLIED INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILTY OR FITNESS FOR PARTICULAR PURPOSE. Wheaton Science Products does not recommend any use that infringes patents. This data and information, and the products and processes described, are subject to change at any time without notice. Please contact your Wheaton Science Products representative with your specific packaging needs.

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Wheaton follows the Society of the Plastics Industry guidelines for marking plastic containers with the appropriate resin identification code numbers as shown below:

Wheaton Science Products mold-marks our containers on the bottom with the appropriate resin identification code. These codes are to assist in identifying material used to manufacture ware to aid in recycling efforts.

Recycling Plastic

PP PS OTHER

PETE HDPE V LDPE

1 = PETE (polyethylene terephthalate) (PET)

2 = HDPE (high density polyethylene)

3 = V (vinyl/polyvinyl chloride) (PVC)

4 = LDPE (low density polyethylene)

5 = PP (polypropylene)

6 = PS (polystyrene)

7 = Other

PET Media Bottle

187


Plastics

The following Plug Styles can be used with Wheaton magnetic stirrers, roller culture apparatus, incubators and pumps.

Bahrain Kuwait Tanzania Belgium Malaysia Trinidad Bermuda Mauritius & Tobago Cyprus Malta United Arab England Nigeria Emirates Myanmar N. Ireland United Kingdom Ghana Oman Wales Hong Kong Qatar Zambia Ireland Scotland Zimbabwe Iraq Singapore Zimbabwe Jordan Sudan (Rep. of)

Bahamas Guatemala Philippines Bolivia Haiti Puerto Rico Brazil Honduras Saudi Arabia Canada Jamaica Singapore Costa Rica Korea, S Sweden Dominican Rep. Libya Taiwan El Salvador Nicaragua United States Ecuador Norway Venezuela Guam Panama Virgin Islands Peru

Bolivia Japan

Austria Indonesia Singapore Belgium Latvia Sweden Bolivia Lithuania Spain Bulgaria Luxembourg Tahiti Czech. Rep Mozambique Turkey Estonia Netherlands USSR (former) Finland Poland Vietnam France Portugal Yugoslavia (former) Germany Romania Zaire (Rep. of) Greece Russia Hungary Slovakia Iceland Slovenia

Denmark Greenland

Israel Jamaica

Switzerland

Argentina Australia China (People’s Rep. of) Fiji New Zealand Papua New Guinea Uruguay

Chile Italy

Bangladesh India Pakistan South Africa Sri Lanka Uganda

Additional plug styles are available through special order. Please contact Technical Service for additional information.

188Plug Styles


189


AlphabeticalA

ABS Plates ...................................................................................................... 47Absorber, Tube ...........................................................................................67-68AC Round Bottle ................................................................................................ 7Acura Micropipettes (see Pipettes)Acurex Bottle Top Dispensers (see Dispensers)Adapters

Bottle Top ............................................................................................. 101Clear-Seal Connecting ............................................................................. 66Connecting ............................................................................................. 66Thermometer .......................................................................................... 66

Air Powered Crimper ....................................................................................... 35Alarm System ................................................................................................. 60Alcohol Burner .............................................................................................. 137Alternative Feed Sources ............................................................................... 105Aluminum Seals (see Caps & Closures)Aluminum Weighing Dishes ............................................................................. 82AMPULES ............................................................................................. 1-2, 107

Cryule Crogenic ........................................................................................ 1Vacule, Lyophilization ......................................................................... 1,107Pre-Scored ........................................................................................... 107

Arsine Generators ........................................................................................... 67Aspiration Feed Sources ................................................................................ 105Autosampler Vials

Convenience Packs ..................................................... 87-88, 90-92, 94-95Crimp Top ......................................................................................... 88, 89E-Z Vial ............................................................................................ 88, 89Headspace ............................................................................................. 91Limited Volume Insert (LVI) ....................................................................... 92Plastic with Glass Inner Cone ................................................................... 93Polypropylene ......................................................................................... 93Shell ...................................................................................................... 87Snap Caps ............................................................................................. 89

Autosampler Vial Selector Guide ..................................................................83-84Autosampler Vial Sizer ................................................................................85-86

BBalsam Bottle ............................................................................................... 137Bar Coded Containers .................................................................................... 133Barnes Dropping Bottles .................................................................................... 5Battery Backup Systems ............................................................................ 60, 63Bench Top Roller Rack ..................................................................................... 58Bioreactors ................................................................................................54-56Biostir Magnetic Stirrers .................................................................................. 53Bleed Tube ................................................................................................ 70, 72BOD Bottles & Accessories .............................................................................3-4

Aerator ..................................................................................................... 4Cap .......................................................................................................... 4Rack ........................................................................................................ 4Stopper Leash .......................................................................................... 4

Bomb Sampler, Stainless Steel ....................................................................... 129BOTTLES ....................................................................................................3-28

AC Round ................................................................................................. 7Balsam................................................................................................. 137Blake ..................................................................................................... 27BOD ......................................................................................................... 3Boston Round ........................................................................................7-8Cylinder Round, Plastic ............................................................................ 26Diagnostic .................................................................................18, 22, 108Dispensing, LDPE.................................................................................... 22Dropping ...........................................................................................23-25Dropping, Barnes ...................................................................................... 5Dropping, Glass, Amber ............................................................................. 5Dropping, Glass, Clear ............................................................................... 5Dropping, LDPE .................................................................................23-25Dropping, with Extended Controlled Tip..................................................... 25Dropping, with Filter Dropping Tip ............................................................ 25Dropping, with Streaming Tip ................................................................... 25Dropping, Vial File ................................................................................... 25Extraction ................................................................................................. 5French Squares ........................................................................................ 8General Purpose Plastic .....................................................................26-28General Purpose Glass .........................................................................6-11

BOTTLES , continued… Graduated Valumetric .............................................................................. 10

Hybridization ........................................................................................... 11 Jars, Straight-Sided, Clear ......................................................................... 9 Jug, HDPE .............................................................................................. 26 Jugs, Glass............................................................................................. 16 Lab 45 ........................................................................................... 13, 108 Lab/Media ................................................................................... 12-13, 30 Labeled Reagent ..................................................................................... 14 Labeled, Safety Coated............................................................................ 17 Leak Resistant ........................................................................................ 29 Media ......................................................................................... 12-13, 30 Mounting Media .................................................................................... 137 Narrow Mouth, Plastic ............................................................................. 29 Oil Sample.............................................................................................. 10 PET, Media, Round .................................................................................. 26 PET, Media, Square ................................................................................. 30 Plastic ...............................................................................................21-30 Reagent, All Glass ................................................................................... 14 Reservoir, Safety Coated .......................................................................... 15 Roller Culture.......................................................................................... 15 Round Bottles, Plastic ............................................................................. 26 Safety Coated, Amber.............................................................................. 16 Safety Coated, Clear ............................................................................... 16 Safety Coated, Lab/Media........................................................................ 17 Safety Coated, Reservoirs ........................................................................ 15 Safety Coated, Solution ........................................................................... 17 Sample, for Sub-Surface Grab Samplers .........................................131-132 Sample, Glass, Oil ................................................................................... 10 Sample, Graduated ................................................................................. 10 Screw Neck Diagnostic...............................................................18, 22, 108 Serum, Amber ................................................................................ 19, 109 Serum, Clear .................................................................................. 19, 109 Serum, HDPE .......................................................................................... 20 Serum, Polypropylene ............................................................................. 19 Snap Cap ............................................................................................... 20 Solution, Safety Coated ........................................................................... 17 Spray ..................................................................................................... 30 Square, Plastic........................................................................................ 30 Standard Wide Mouth ................................................................................ 9 Stoppered, Glass..................................................................................... 14 Straight Sided Jar ..................................................................................... 9 TCLP Extraction ........................................................................................ 5 Uni-Dose, Glass, Amber ........................................................................... 20 Uni-Dose, Plastic .................................................................................... 20 Valumetric .............................................................................................. 10 Wide Mouth Blake Packer, HDPE .............................................................. 27 Wide Mouth Round Packer, HDPE ............................................................ 27 Wide Mouth Jars, Polypropylene .............................................................. 28 Wide Mouth Jars, Polystyrene .................................................................. 28 Wide Mouth Jars, HDPE .......................................................................... 28 Wide Mouth, Glass, Amber ...................................................................... 11 Wide Mouth, Glass Stoppered .................................................................. 14 Wide Mouth, Packers, Amber ................................................................... 11 Wide Mouth, Glass, Clear ................................................................. 7, 9-10 Wide Mouth, Plastic ...........................................................................27-28

Boerner Micro Test Slide ................................................................................ 137 Boston Round Bottle .......................................................................................7-8 Bottle Stoppers ................................................................................................. 3 Bottle Top Dispensers ...............................................................................99-106 Boxes, Cryule ................................................................................................ 142 Boxes, Vial .................................................................................................... 149 Brushes, Cleaning ......................................................................................... 105 Burners, Alcohol ............................................................................................ 137

C Calibra Digital Macropipettes ..................................................................117-118 Calibrex Digital 520 & 521 Bottle Top Dispenser ........................................99-100 Cannulas, Dispensing .............................................................................105-106 CAPS & CLOSURES ...................................................................................31-42

Pennyhead ............................................................................................... 3 Robotic .................................................................................................... 3 Snap-On ................................................................................................ 34 Straight Plug......................................................................................... 110

190Alphabetical


CAPS & CLOSURES, continued… Thin Flange .................................................................................... 18, 108 Ultra Pure ............................................................................................. 110

Aluminum Seals ............................................................................................ 32 Flip Cap ................................................................................................. 33 Lined ..................................................................................................... 32 Septa ..................................................................................................... 33 Unlined .......................................................................................... 32, 109 Tamper Evident ................................................................................. 20, 32

Cap Liner Liner, TLCP ............................................................................................... 5 Specifications ..................................................................................... 6, 31

Screw Caps ...................................................................................... 37-42, 110 Autoclavable ........................................................................................... 39 Black Phenolic ..................................................................12-13, 17, 37-38 Cap Liner Specifications .......................................................................... 31 Colored .................................................................................................. 42 Dropper Bottle Caps ................................................................................ 24 E-Z Ex-Traction ....................................................................................... 41 Glass Filled ............................................................................................. 41 I-Loc .........................................................................................18, 42, 108 Inserts .................................................................................................... 38 Lab 45 ................................................................................13, 17, 41, 108 Liquid Scintillation ................................................................................... 38 Microlink ................................................................................................ 39 Open Top ...........................................................................................38-39 PBT ....................................................................................................... 41 Polybutylene Terephthalate ...................................................................... 41 Polyproylene ......................................................................................40-42 Pour Rings ................................................................................13, 41, 108 Septa ..................................................................................................... 38 Snap Cap ......................................................................................... 20, 42 Solid Top ...........................................................................................37-42 TLCP ........................................................................................................ 5 Vented Cap ...................................................................................... 15, 42

Stoppers, Rubber .....................................................................................33-34 2 & 3-Leg ................................................................................. 33-34, 110 Ampule, 11mm ................................................................................. 1, 107 Complete Coat ................................................................................ 33, 110 Fluorinated Polymer ........................................................................ 33, 110 Gray Chlorobutyl ................................................................................... 110 Gray Chlorobutyl-Isoprene Blend ............................................................ 110 Gray Halobutyl ...................................................................................... 110 Igloo .............................................................................................. 33, 110 Lyophilization .....................................................................13, 18, 108, 110 Natural Red Rubber................................................................................. 34 Premium ........................................................................................ 33, 110 Sleeve Style ............................................................................................ 34 Snap-On ................................................................................................ 34 Straight Plug................................................................................... 34, 110 Teflon Faced ........................................................................................... 34 Thin Flange ...............................................................................18, 34, 108 Ultra Pure ....................................................................................... 33, 110

CART (Computer Aided Roller Technology) ......................................................... 63 CELL CULTURE

Battery Backup ....................................................................................... 60 Bench Top .............................................................................................. 58 Biopro PC Software ................................................................................. 57 BioStir Magnetic Stirrer ........................................................................... 53 Bottles, Roller ......................................................................................... 15 CART2

.................................................................................................... 63 Cell Optimizer System ............................................................................. 54 Celstir .................................................................................................... 51 Cold Finger ....................................................................................... 54, 55 Control Tower ......................................................................................... 56 Cost Buster Roller Apparatus ................................................................... 60 C-Tubes ................................................................................................. 49 Flasks .................................................................................................... 49 Flasks, Trypinizing ................................................................................... 50 Heating Wrap.....................................................................................54-55 High Volume Production Roller Apparatus ............................................59-63 Incubator, CO2

........................................................................................ 64 Incubator, Roll-in ..................................................................................... 64 Magna Flex Microcarrier .......................................................................... 52

CELL CULTURE, continued… Magnetic Stirrers .................................................................................... 53 MantaRay ............................................................................................... 51 Micro-Stir Magnetic Stirrer ...................................................................... 53 Mini Bottle Roller Apparatus ..................................................................... 58 Mini Pilot Plant ....................................................................................... 55 Modular Cell Production Roller Apparatus ................................................. 61 Overdrive Cell Culture System .................................................................. 56 Production Roller Apparatus................................................................59-63 R2

P, Research to Production Roller Apparatus ........................................... 62 Remote Control ....................................................................................... 53 Roller Culture Apparatus .....................................................................58-63 Roll-In Incubator ..................................................................................... 64 Rotation Alarm System ............................................................................ 60 Small Bottle Roller Apparatus ................................................................... 58 Software ................................................................................................ 63 Spinner Flasks ...................................................................................51-52 Stirrers, Magnetic ................................................................................... 53 Tissue Grinders ..................................................................................43-46

Cell Optimizer System...................................................................................... 54 Cell Production Roller Apparatus .................................................................59-63 Celstir Spinner Flasks ...................................................................................... 51 Chambers, TLC Developing .............................................................................. 97 CHEMISTRY GLASSWARE .........................................................................65-81

Arsine Generator ..................................................................................... 67 Chemistry Kits ...................................................................................70-72 Clamps .................................................................................................. 65 Clear-Seal Connecting Adapter ................................................................ 66 Clear-Seal Grease Free Joints .................................................................. 66 Clear-Seal Outer Joints ........................................................................... 66 Cyanide Distillation Kits ........................................................................... 67 Diazomethane Generators ....................................................................... 68 Kuderna Danish Concentors ...............................................................68-69 Micro Chemistry Kit ............................................................................70-71 Semi-Micro Chemistry Kit ........................................................................ 72 Soxhlet Extraction Apparatus ................................................................... 69 Thermometer Adapter ............................................................................. 66 The Wheaton Connection......................................................................... 65 Wheaton Micro Kit .............................................................................70-71 Wheaton Semi-Micro Kit.......................................................................... 72

Chemistry Kits............................................................................................70-72 CHROMATOGRAPHY ..................................................................................83-98

ABC Screw Cap ...................................................................................... 94 ABC Vials ............................................................................................... 94 Aluminum Seals, 11 mm ......................................................................... 89 Autosampler Vial Selector Guide .........................................................83-84 Autosampler Vial Sizer ........................................................................85-86 Autosampler Vials ..............................................................................83-96 Chromatographic Spray Bottle ................................................................. 98 Convenience Packs ..................................................... 87-88, 90-92, 94-95 Crimp Top Vial, Standard Opening ............................................................ 89 Crimp Top Vials .......................................................................88-90, 92-93 Crimp/Snap Vial ................................................................................ 89, 93 Developing Chambers ............................................................................. 97 E-Z Aluminum Seal, 11 mm ..................................................................... 89 E-Z Vial with Snap/Crimp Top ................................................................... 89 E-Z Vials................................................................................................. 88 Headspace Pressure Release Seals .......................................................... 91 Headspace Vials ..................................................................................... 91 LC Column ............................................................................................. 97 Limited Volume Insert ..............................................................87-89, 94-95 LVI Vials.............................................................................................92-93 Plastic Vials ............................................................................................ 93 Reagent Sprayer ..................................................................................... 98 Sample Recovery Tube ............................................................................ 97 Screw Cap Vials .................................................................................92-96 Screw Caps, ABC .................................................................................... 94 Shell Vials............................................................................................... 87 Snap Caps, 11 mm ................................................................................. 89 Snap Plug Caps ...................................................................................... 87 Snap Ring Vial, Plastic ............................................................................. 93 Snap Ring/Crimp Top Vial ........................................................................ 89 Staining Jar ............................................................................................ 97 TLC Developing Tank ............................................................................... 97

191


AlphabeticalCHROMATOGRAPHY, continued…

TLC Reagent Sprayer .............................................................................. 98TLC Sample Recovery Tube ..................................................................... 97

ClampsAluminum..........................................................................................76-79Standard Taper Clamps ........................................................................... 65

Cleaning Brushes .......................................................................................... 105Clear-Seal Joints ........................................................................................66-70Closures (see Caps & Closures)Coliwasa ................................................................................................129-130Columbia Jar ................................................................................................ 136Content ID Label ............................................................................................... 6Control Tower .................................................................................................. 56Coplin Jars ................................................................................................... 137Crimpers

Air Powered ............................................................................................ 35Crimping Station, Crimpmaster ................................................................ 35E-Z Crimper...................................................................................... 35, 96Uni-Dose Bottle/Vial .......................................................................... 20, 35

Critically Clean Containers ............................................................................. 134Cryogenic Ampule ............................................................................................. 1Cryogenic Vials ......................................................................................140-141Cryogenic Vial Rack ....................................................................................... 142Cryule Cryogenic Ampule ................................................................................... 1C-Tubes.......................................................................................................... 49Custom Containers ........................................................................................ 133Customized Services ..............................................................................133-134Cyanide Distillation Kits...............................................................................67-68Cyanide Scrubber Distillation Kit ....................................................................... 68Cylinder Round Bottles .................................................................................... 26

DDecappers ........................................................................................... 35-36, 96Deck Kit .................................................................................................... 58, 62Desiccant Packs.............................................................................................. 74Desiccators ................................................................................................73-74Developing Chamber, TLC ................................................................................ 97Diagnostic Bottles ..............................................................................18, 22, 108Diazomethane Generator ................................................................................. 68Dilution Vial ................................................................................................... 142Dishes

Micro Weighing, Aluminum ...................................................................... 82Staining................................................................................................ 136

DISPENSERS ..........................................................................................99-106Acurex compact 501/502 ..................................................................... 100Bottle Top ........................................................................................99-100Calibrex digital 520 ................................................................................. 99Calibrex digital 521 ............................................................................... 100Dosys Syringes ..............................................................................103-106

Low Profile Bottle Top ............................................................................ 100Microdispenser Pipette .......................................................................... 122Omnispense Plus Pump ........................................................................ 126

Self-Refilling Microdispenser ................................................................. 122 Self-Refilling Syringe ......................................................................103-106

Socorex ...........................................................................................99-106Step-Pette, 411 .................................................................................... 121Unispense Pump ................................................................................... 127

Dispensing Cannulas ..................................................................................... 105Disposable Tissue Grinder, Micro ...................................................................... 46Distillation

Column .............................................................................................70-72Cyanide .............................................................................................67-68

Dosys Syringes ......................................................................................103-106Double Sidearm Celstir .................................................................................... 51Dounce Tissue Grinder................................................................................43-44Dropper

Bottles ..............................................................................................23-25Bottle Caps ............................................................................................. 24Bottle Tips .............................................................................................. 24Tips ....................................................................................................... 24Tip for Serum Bottle ................................................................................ 20Tip Caps ................................................................................................. 24

Dropping Bottles ........................................................................................23-25Drum Opener ................................................................................................ 132

Drum Thieves ................................................................................................ 130 Dry Seal Desiccators ....................................................................................... 73 Dura-Grind Tissue Grinder ............................................................................... 44

E E-C Sample Vials ....................................................................................151-152 EPA Vial ........................................................................................................ 142 Evaporative Concentrators ............................................................................... 69 E-Z Aluminum Seals ........................................................................................ 89 E-Z Extraction Vial ......................................................................................... 143 E-Z Sampler ................................................................................................. 130 Erlenmeyer Flasks ........................................................................................... 49 Extended Controlled Dropper Tip ...................................................................... 24 Extraction Bottle ................................................................................................ 5 Extraction Thimble ........................................................................................... 69

F Feed Tubing .................................................................................................. 105 Filtration Apparatus, Glass ..........................................................................75-80

Assemblies ........................................................................................76-79 Clamps .............................................................................................76-79 Filter Flasks .......................................................................................76-79 Filtration Transfer Funnel ......................................................................... 80 Flask, Filter ............................................................................................. 80 Funnel, Glass .....................................................................................76-80 Transfer Funnel ....................................................................................... 80 Vacuum Filter Assemblies, 25 mm ........................................................... 76 Vacuum Filter Assemblies, 47 mm ......................................................77-79 Vacuum Filter Assemblies, 90 mm ........................................................... 80

Flasks Celstirs..............................................................................................51-52 Culture, Suspension ...........................................................................51-52 Erlenmeyer ............................................................................................. 49 Filter .................................................................................................76-80 Filter, Safety Coated ................................................................................ 80 Jacketed ................................................................................................ 52 Magna Flex ............................................................................................ 52 MantaRay ............................................................................................... 51 Microcarrier Spinner ............................................................................... 52 Nephelo Culture ...................................................................................... 49 Shake .................................................................................................... 49 Suspension Culture ............................................................................51-52 Trypsinizing ............................................................................................ 50

Footswitch for Pumps .............................................................................126-127 Freestanding Cryogenic Vials ......................................................................... 140 Freezer Boxes ............................................................................................... 142 French Square Bottle ......................................................................................... 8 Funnel-Top Ampule ........................................................................................... 2 Funnels, Filtration .......................................................................................76-80

G General Purpose Plastic Bottles ...................................................................26-28 Generators, Arsine ........................................................................................... 67 Generator, Diazomethane ................................................................................. 68 Glass Filtration Apparatus ...........................................................................75-80 Glass Sinker ................................................................................................. 128 Grab Sampler I, II and III ..........................................................................130-131 Graduated Volumetric Bottle ............................................................................. 10 Grip, Slide ..................................................................................................... 137

H Hazard Communication Label ............................................................................. 6 Headspace Vials .............................................................................................. 91 High Recovery Vial ..................................................................................143-145 Homogenizer................................................................................................... 47 Hybridization Bottles (Tubes) ............................................................................ 11 Hybridization Bottles Rack ................................................................................ 11

I I-LOC Closures ........................................................................................ 42, 108 Imhoff Cones/Rack .......................................................................................... 82

192Alphabetical


Impellers ........................................................................................................ 52 Incubators ...................................................................................................... 64 Injection Needles ........................................................................................... 105 Inserts, Cryule Cap ........................................................................................ 142

J Jacketed Double Sidearm Celstir ...................................................................... 52 Jars

Coplin Staining ..................................................................................... 137 Staining................................................................................................ 137 Straight Sided, Clear ................................................................................. 9 Wide Mouth, Polypropylene ..................................................................... 28 Wide Mouth, Polystyrene ......................................................................... 28 Wide Mouth, HDPE .................................................................................. 28

Jugs Safety Coated ......................................................................................... 16 Square HDPE .......................................................................................... 26

K Kits

Cyanide Distillation .................................................................................. 67 Cyanide Scrubber Distillation ................................................................... 68 Organic & Environmental Chemistry ....................................................67-72 Semi-Micro ............................................................................................ 72 Tissue Grinder ........................................................................................ 46 Microscale.............................................................................................. 70 Joints, Sleeves & Clamps ...................................................................65-66

Kuderna-Danish Concentrators ...................................................................68-69

L Lab 45 Media Bottles .............................................................................. 13, 108 Lab/Media Bottles ................................................................................... 30, 108 Lab Files, Sample Vials ...........................................................................150-151 Label, Content ID............................................................................................... 6 Label, Hazard Communication ............................................................................ 6 Laboratory & Organic Glassware .................................................................65-72 LC Column...................................................................................................... 97 Leak Resistant Bottles ..................................................................................... 29 Limited Volume Inserts (see Chromatography) Liquid Scintillation Vials ..........................................................................146-148 Low Profile Bottle Top Dispensers ................................................................... 100 LYOPHILIZATION

2-Leg Stopper ...................................................................................... 110 3-Leg Stopper ...................................................................................... 110 Aluminum Seals .................................................................................... 109 Ampules ............................................................................................... 107 Black Lyophilization Stopper .................................................................. 108 Complete Coat Fluorinated Polymer Stoppers ......................................... 110 Diagnostic Bottles ................................................................................. 108 EPDM Stopper ...................................................................................... 108 Fluorinated Polymer Stoppers ................................................................ 110 Halobutyl/60 Stopper ............................................................................ 108 Igloo Stopper ........................................................................................ 110 I-LOC Closure ....................................................................................... 108 Lab 45 Bottle ........................................................................................ 108 Screw Neck Diagnostic Bottles............................................................... 108 Serum Bottles ....................................................................................... 109 Serum Vials .......................................................................................... 109 Thin Flange Stopper .............................................................................. 108 Ultra Pure Premium Stoppers................................................................. 110 Vacule Vial .................................................................................... 110, 154

Lyophilization Process.................................................................................... 107 Lyophilization Stoppers .................................................................................. 110

M Macropipettes (see Pipettes) Magna Flex Microcarrier Spinner Flask ............................................................. 52 Magnetic Stirrers............................................................................................. 53 Manifolds, Microtest ...................................................................................... 105 MantaRay ....................................................................................................... 51

Media/Lab Bottles ..................................................................................... 13, 30 Micro

Concentrator .......................................................................................... 69 Kits ...................................................................................................70-72 Snyder Column ....................................................................................... 69 Soxhlet ................................................................................................... 69

Micro-Stir Magnetic Stirrers ............................................................................. 53 Microcarrier Spinner Flasks ............................................................................. 52 Micropipettes (see Pipettes) Microtest Manifolds ....................................................................................... 105 Microtiter Plate, 96 Well..............................................................................47-48 Microtiter Plates

96 Well Multi-Tier ................................................................................... 47 ABS Plates ............................................................................................. 47 Multi-Tier Sections .................................................................................. 48 Sealing Mat ............................................................................................ 48 Sealing Strips and Plugs .......................................................................... 48 Solid Clear Cover .................................................................................... 48 Topas COC Plates ................................................................................... 48 Vial Loader ............................................................................................. 48 Vials for Multi-Tier System ....................................................................... 48

Micro Sample Vial ......................................................................................... 148 Micro Tissue Grinder Kit ................................................................................... 46 Micro Tissue Grinder with Cap.......................................................................... 46 Mini-Files ..................................................................................................... 153 Mini-Pilot Plant System .................................................................................... 55 Modular Cell Production Roller Apparatus ......................................................... 61 Mounting Media Bottle................................................................................... 137 M-T Vial File ......................................................................................... 149, 153 Multi-Tier Section for Microplates ..................................................................... 48

N Needles, Injection .......................................................................................... 105 Nephelo Culture Flasks .................................................................................... 49 NextGen V Vials ......................................................................................144-145 Nozzle .......................................................................................................... 128

O Oil Sample Bottle ............................................................................................ 10 Omni-Vials .................................................................................................... 147 Omnispense Plus Pump ................................................................................. 126 Opener, Drum ............................................................................................... 132 Organic & Environmental Chemistry Kit ........................................................70-72 Outer Joints .................................................................................................... 66 Overdrive Cell Culture System .......................................................................... 56 Overhead Stirrer .............................................................................................. 47

P Pasteur Pipettes ............................................................................................ 120 Peristaltic Pumps .......................................................................................... 125 PET (Polyethylene Terephthalate)

Liquid Scintillation Vials ......................................................................... 147 Media Bottles ................................................................................... 26, 30 Uni-Dose Vials ........................................................................................ 20

PIPETTES ..............................................................................................111-124 Acura electro 925 Micro .................................................................115-116 Acura electro 935 Macro ................................................................115-116 Acura electro 955 Multi ..................................................................115-116 Acura electro Basic Package ................................................................. 116 Acura electro Battery Pack .................................................................... 116 Acura electro Charging Stand ................................................................ 116 Acura electro Volumetric Modules .......................................................... 116 Acura manual 810 Dilution .................................................................... 114 Acura manual 815 Fixed ....................................................................... 113 Acura manual 825 Micro ................................................................111-112 Acura manual 835 Macro ...............................................................111-112 Acura manual 855 Multi .................................................................111-112 Acura manual Triopack .......................................................................... 113 Acura Positive Displacement Model 841 ................................................. 121 Acura Self-Refilling Microdispenser ........................................................ 122 Calibra Digital 822 Micro ................................................................117-118 Calibra Digital 832 Macro ...............................................................117-118

193


AlphabeticalPIPETTES, continued...

Calibra Digital 852 Multi .................................................................117-118Pasteur Pipettes ................................................................................... 120Pipette Tips .......................................................................................... 119

Profiller electro 446 .............................................................................. 123 Profiller manual 435-436 ...................................................................... 124

Socorex .........................................................................................111-124Stepper 411 Repeater ........................................................................... 121

Pipette, AccessoriesDisposable Pasteur Pipettes .................................................................. 120Nozzle Filters ........................................................................................ 120Pasteur Pipette Adapter ......................................................................... 120Pasteur Pipettes ................................................................................... 120Pipette Stand ........................................................................................ 120Reagent Reservoirs ............................................................................... 120Smartie Caps ........................................................................................ 120Socorex Pipette Work Station ................................................................. 120Universal Work Station .......................................................................... 120Work Station 337 .................................................................................. 120Work Station 340 .................................................................................. 120

Plier Decapper ................................................................................................ 36Polypropylene Screw Cap (see Caps & Closures)Potter-Elvehjem Tissue Grinders ..................................................................44-45Pour Ring

ETFE .............................................................................................. 13, 108Polypropylene ................................................................................. 13, 108

Pre-Scored Ampule ................................................................................... 1, 107Pre-Weighed Containers ................................................................................ 134PRODUCT APPLICATIONS ......................................................................155-165Production Equipment, Cell Culture ....................................................... 55, 59-64Production Volume Roller Apparatus ............................................................59-63PUMPS

Comparison Chart ................................................................................. 125Footswitch .....................................................................................126-127Hose Barb ............................................................................................ 128Nozzle .................................................................................................. 128Omnispense Plus .................................................................................. 126Peristaltic ......................................................................................125-126Sinker, Glass ......................................................................................... 128Tubing .................................................................................................. 127Unispense ............................................................................................ 127

RR

2P Roller Culture Apparatus ............................................................................ 62

RacksBOD Bottle ............................................................................................... 4Hybridization ........................................................................................... 11Imhoff Cone ............................................................................................ 82Vial ...................................................................................................... 149

Reagent Bottles, All Glass ................................................................................ 14Reagent Bottles, Screw Cap ............................................................................. 14Reagent Sprayers, TLC .................................................................................... 98Recovery Tube, TLC ......................................................................................... 97Remote Control ............................................................................................... 53Repeater Pipette ........................................................................................... 121Repetitive Syringe ..................................................................................103-106Replacement Screw Caps

AC Round Bottles ...................................................................................... 7Boston Round Bottles ................................................................................ 8French Squares ........................................................................................ 9Liquid Scintillation Vials ......................................................................... 148Standard Wide Mouth Bottles..................................................................... 9Straight Sided Jar ................................................................................... 10Valumetric .............................................................................................. 10Wide Mouth Packer ................................................................................. 11Media Bottles ......................................................................................... 13Lab 45 ................................................................................................... 13Sample Vials ......................................................................................... 152

Reservoir, High Capacity .................................................................................. 15 Resin Identification Code ......................................................................... 22, 187

Robotic Stoppers ............................................................................................... 3Roll-In Incubator .............................................................................................. 64Roller Culture Apparatus

88 Position R2P System ........................................................................... 63

Apparatus .........................................................................................58-63 Battery Backup Systems .................................................................... 60, 63 Bottles ................................................................................................... 15 CART (Computer Aided Roller Technology) ................................................ 63 Cost Buster Roller Apparatus ................................................................... 60 Deck Kit ................................................................................................. 58 Mini Roller Apparatus .............................................................................. 58 Modular Cell Production Roller Apparatus ................................................. 61 Production Volume Roller Apparatus ....................................................61-62 Roll-In Incubator ..................................................................................... 64 Rotation Alarm ........................................................................................ 60 Small Bottle Roller Apparatus ................................................................... 58

Rotation Alarm System .................................................................................... 60 Rubber Stoppers (see Caps & Closures)

S Safe-Grind Tissue Grinders .............................................................................. 44 Safety Caps, Tamper-Evident ............................................................................ 32 Safety Coated

Bottles ..............................................................................................15-17 Filter Flasks ............................................................................................ 80 Lab 45 Bottles ........................................................................................ 17 Labeled Bottles ....................................................................................... 17 Media Bottles ......................................................................................... 17 Multi-Purpose Reservoirs ........................................................................ 15 Reservoir, High Capacity .......................................................................... 15 Solution Bottles ....................................................................................... 17 Tissue Grinders ....................................................................................... 44

Sample Recovery Tube .................................................................................... 97 Sample Vials ..........................................................................................150-154 SAMPLE COLLECTION ...........................................................................129-132

Bomb Sampler, Stainless Steel ............................................................... 129 Bottle, Sample ...............................................................................131-132 Coliwasa .......................................................................................129-130 E-Z Sampler ......................................................................................... 130 Grab Sampler I, II, and III ................................................................130-131 Scoop, Stainless Steel ........................................................................... 132 Trier Sampler ........................................................................................ 132

SciLutions Customized Services ..........................................................133-134 Custom Containers ............................................................................... 133 Bar Coding ........................................................................................... 133 Critical Cleaning.................................................................................... 134 Surface Treatment................................................................................. 134 Taring .................................................................................................. 134

Scintillation Vials ....................................................................................146-148 Caps .................................................................................................... 148 Vial Racks ............................................................................................ 149

Scoop, Stainless Steel ................................................................................... 132 Screw Caps (see Caps & Closures) Screw Neck Diagnostic Bottle .................................................................. 18, 108 Scrubber ........................................................................................................ 68 Seals (see Caps & Closures) Sealing Mat for Microplate System ................................................................... 48 Self-Refilling Syringes .............................................................................103-106 Semi-Micro Organic Chemistry Kit .................................................................... 72 Septa (see Caps & Closures) Serum Bottles .................................................................................... 19-20, 109 Serum Dropper Tip .......................................................................................... 20 Serum Vials ............................................................................................ 19, 109 Shake Flasks .................................................................................................. 49 Shorty Vials ............................................................................................150-151 Silicone Tubing .............................................................................................. 127 Sinker, Glass ................................................................................................. 128 Slide Cleaning ............................................................................................... 138 Slide Coating ................................................................................................ 138 Slide Grip ...................................................................................................... 137 Smartie Caps ................................................................................................ 120 Snap Cap Bottles ............................................................................................ 20 Snap Caps ...................................................................................................... 20 Snapper, Ampule ............................................................................................... 2 Snyder Column ............................................................................................... 69 Socorex Dispensers ..................................................................................99-106 Socorex Pipettes (see Pipettes) Socorex Work Stations ............................................................................120-122

194Alphabetical


Software Biopro PC ............................................................................................... 57 CART ..................................................................................................... 63

Solid Determination ......................................................................................... 82 Soxhlet Extractor ............................................................................................. 69 Spin Vane & Magnetic Stir Bar ....................................................................... 145 Spinner Flasks & Systems ...........................................................................51-56 Spray Bottles .................................................................................................. 30 Staining Dishes & Jars............................................................................135-137 STAINING WARE ....................................................................................135-138

Alcohol Burner ...................................................................................... 137 Balsam Bottle ....................................................................................... 137 Boerner Micro Test Slide ........................................................................ 137 Coplin Jar ............................................................................................. 137 Columbia Jar ........................................................................................ 136 Staining Dishes ..................................................................................... 136

Stands Dispenser ............................................................................................. 101 Tubing .................................................................................................. 128 Imhoff Cone ............................................................................................ 82

Standard Taper Clamps ................................................................................... 65 Standard Wide Mouth Bottle .............................................................................. 9 Step-Pette, 411 ............................................................................................ 121 Sterile PET Bottle ............................................................................................ 26 Stirrer, Overhead ............................................................................................. 47 Stirrers, Magnetic ............................................................................................ 53 Stoppers (see Caps & Closures) Straight Sided Jar ............................................................................................. 9 Sub Surface Grab Samplers ....................................................................130-131 Surface Treated Containers ............................................................................ 134 Suspension Culture

Flasks ...............................................................................................51-52 Stirrers ................................................................................................... 53

Syringes Dosys ............................................................................................103-106 Replacement ........................................................................................ 106 Barrels ................................................................................................. 106 Pistons ................................................................................................. 106 PVC Protection Sleeves ......................................................................... 106

T Tamper Evident Safety Caps ............................................................................. 20 Tapered Tissue Grinder .................................................................................... 45 Taring Containers .......................................................................................... 134 TCLP Extraction Bottles, Caps & Liners ............................................................... 5 TECHNICAL DATA ..................................................................................166-188 Temperature Monitor ....................................................................................... 63 Tenbroeck Tissue Grinder ................................................................................. 46 Thieve, Drum ................................................................................................ 130 Tips

Dropper .................................................................................................. 24 Tissue Grinders

Disposable, Micro ................................................................................... 46 Dounce .............................................................................................43-44 Dura-Grind ............................................................................................. 44 Kit .......................................................................................................... 46 Micro ................................................................................................45-46 Plastic Coated ........................................................................................ 44 Potter-Elvehjem .................................................................................44-45 Safe-Grind .............................................................................................. 44 Stirrer .................................................................................................... 47 Tapered .................................................................................................. 45 Tenbroeck .............................................................................................. 46

TLC Developing Chamber ............................................................................... 97 Sample Recovery Tube ............................................................................ 97 Sprayer, Reagent .................................................................................... 98

Topas COC Plates ............................................................................................ 48 Trier Sampler ................................................................................................ 132 Trypsinizing Flasks .......................................................................................... 50 Tubes

Culture C-Tube ....................................................................................... 49 Drying .................................................................................................... 67 Hybridization ........................................................................................... 11

Sample Recovery .................................................................................... 97 Scrubber/Absorber .............................................................................67-68

Tubing Extension ............................................................................................. 101 Feeding ................................................................................................ 105 Silicone ................................................................................................ 127 Support Stand ...................................................................................... 128 Viton .................................................................................................... 128

U Uni-Dose Bottles ............................................................................................. 20 Uni-Dose Vials................................................................................................. 20

V V Vials ...................................................................................................144-145 Vacule Lyophilization Ampule .................................................................. 1-2, 107 Vacuum Desiccators, Dry-Seal ....................................................................73-74 Vacuum Filtration Assemblies ......................................................................75-80 Value Added Services (see SciLutions) Valumetric, Graduated Bottle ............................................................................ 10 Vented Cap, 45 mm ........................................................................................ 15 VIALS

Autosampler ......................................................................................83-96 Ampules ................................................................................................1-2 Cryogenic ......................................................................................140-142 Culture ................................................................................................. 142 Dilution ................................................................................................ 142 E-C Sample Vial .............................................................................151-152 EPA ...................................................................................................... 142 E-Z Ex-Traction ..................................................................................... 143 High Recovery ...............................................................................143-145 Lab Files .......................................................................................150-151 Liquid Scintillation ..........................................................................146-148 Micro Sample ....................................................................................... 148 Mini-File ............................................................................................... 153 M-T Vial File ................................................................................. 149, 153 NextGen V Vial ...............................................................................144-145 Omni-Vial ............................................................................................. 147 Racks................................................................................................... 149 Sample .........................................................................................150-154 Sampule............................................................................................... 148 Serum ............................................................................................ 19, 109 Vacule .................................................................................................. 154 Vial File .........................................................................................152-153 V Vial ............................................................................................144-145

Vial Files .......................................................................................... 25, 152-153 Vial Loader for Microplate System .................................................................... 48 Vial Racks ..................................................................................................... 149 Vial Selector Guide .....................................................................................83-84 Vial Sizer ...................................................................................................85-86 Vials for Multi-Tier Microplate Systems ............................................................. 48 Viton Tubing .................................................................................................. 127

W Weighing Dishes ............................................................................................. 82 Wheaton Connection ..................................................................................69-72 Wheaton Dry-Seal Desiccator .......................................................................... 73 Wheaton Semi-Micro Kit .................................................................................. 72 Wide Mouth Jars, HDPE ................................................................................... 28 Wide Mouth Jars, Polypropylene ...................................................................... 28 Wide Mouth Jars, Polystyrene .......................................................................... 28 Wide Mouth Packer Bottles .............................................................................. 11 Work Stations ........................................................................................120-122

See page 214 for chemical compatibility of containers, cap liners, cap materials, and rubber stoppers.

195


Numeric100

109933 .............................................. 70110008 .............................................. 71110012 .............................................. 72123753 .............................................. 70123892 .............................................. 72125478 .................................. 65, 69, 71125480 .................................. 65, 71, 72125482 .............................................. 65125486 .................................. 65, 69, 72125488 .............................................. 65125492 .............................................. 65125494 .............................................. 65125496 .............................................. 65125497 .............................................. 65125499 .............................................. 65125500 .............................................. 65125504 ........................................ 65, 69139479 ........................................ 66, 70139506 .............................................. 66139509 .............................................. 66139513 .............................................. 66139626 .............................................. 66139629 .............................................. 66139633 .............................................. 66139656 .............................................. 66139663 .............................................. 66161978 .............................................. 71165996 ........................................ 66, 72168353 .............................................. 70168378 .............................................. 71168388 .............................................. 72176732 ................................................ 2176740 ................................................ 2176759 ................................................ 2176762 ................................................ 2176772 ................................................ 2176776 ................................................ 2176779 ................................................ 2176780 ................................................ 2176782 ................................................ 2176784 ................................................ 2176792 ................................................ 2176796 ................................................ 2176799 ................................................ 2177105 ................................................ 2180020 .............................................. 67180021 .............................................. 67180022 .............................................. 67180023 .............................................. 67180024 .............................................. 67180030 .............................................. 67180034 .............................................. 67

200208890 ............................................ 137208923 .............................................. 29208924 .............................................. 29208925 .............................................. 29208926 .............................................. 29208927 .............................................. 29208928 .............................................. 29208929 .............................................. 29209041 .............................................. 29209043 .............................................. 29209044 .............................................. 29209045 .............................................. 29209046 .............................................. 29209047 .............................................. 29209048 .............................................. 29209049 .............................................. 29209050 .............................................. 29209121 .............................................. 29

211634 .............................................. 25211635 .............................................. 25211636 .............................................. 25211641 .............................................. 25215237 .............................................. 14215239 .............................................. 14215240 .............................................. 14215241 .............................................. 14215243 .............................................. 14215257 .............................................. 14215259 .............................................. 14216017 .............................................. 14216019 .............................................. 14216020 .............................................. 14216021 .............................................. 14216627 .............................................. 17216629 .............................................. 17216631 .............................................. 17216637 .............................................. 17216639 .............................................. 17216641 ...................................... 17, 131216711 ................................................ 5216712 ................................................ 5216731 ................................................ 5219360 ...................................... 18, 108219361 ...................................... 18, 108219362 ...................................... 18, 108219364 ...................................... 18, 108219370 ...................................... 18, 108219371 ...................................... 18, 108219372 ...................................... 18, 108219374 ...................................... 18, 108219415 .............................................. 13219417 .............................................. 13219419 .............................................. 13219420 .............................................. 13219435 .............................................. 12219437 .............................................. 12219439 .............................................. 12219440 .............................................. 12219455 .............................................. 17219457 .............................................. 17219495 .............................................. 13219497 .............................................. 13219499 .............................................. 13219500 .............................................. 13219575 .............................................. 13219577 .............................................. 13219579 .............................................. 13219580 .............................................. 13219715 .............................................. 12219717 .............................................. 12219719 .............................................. 12219720 .............................................. 12219755 .............................................. 12219757 .............................................. 12219759 .............................................. 12219760 .............................................. 12219815 .............................................. 12219817 .............................................. 12219819 .............................................. 12219820 .............................................. 12219845 .............................................. 12219855 .............................................. 12219865 .............................................. 12219875 .............................................. 12219885 .............................................. 13219915 ...................................... 13, 108219917 .............................................. 13219919 .............................................. 13219920 .............................................. 13219921 .............................................. 13219925 .............................................. 13219927 .............................................. 13

209123 .............................................. 29209124 .............................................. 29209125 .............................................. 29209126 .............................................. 29209127 .............................................. 29209128 .............................................. 29209129 .............................................. 29209130 .............................................. 29209161 .............................................. 29209163 .............................................. 29209164 .............................................. 29209165 .............................................. 29209166 .............................................. 29209167 .............................................. 29209168 .............................................. 29209169 .............................................. 29209170 .............................................. 29209425 .............................................. 29209426 .............................................. 29209427 .............................................. 29209428 .............................................. 29209429 .............................................. 29209545 .............................................. 29209546 .............................................. 29209547 .............................................. 29209548 .............................................. 29209549 .............................................. 29209550 .............................................. 29209625 .............................................. 29209626 .............................................. 29209627 .............................................. 29209628 .............................................. 29209629 .............................................. 29209630 .............................................. 29209665 .............................................. 29209666 .............................................. 29209667 .............................................. 29209668 .............................................. 29209669 .............................................. 29209672 .............................................. 27209673 .............................................. 27209674 .............................................. 27209675 .............................................. 27209676 .............................................. 27209683 .............................................. 27209685 .............................................. 27209686 .............................................. 27211194 .............................................. 22211195 .............................................. 22211199 .............................................. 22211523 ................................................ 5211530 ................................................ 5211533 ................................................ 5211590 .............................................. 25211592 .............................................. 25211602 .............................................. 25211603 .............................................. 25211604 .............................................. 25211605 .............................................. 25211606 .............................................. 25211607 .............................................. 25211618 .............................................. 25211620 .............................................. 25211621 .............................................. 25211622 .............................................. 25211623 .............................................. 25211624 .............................................. 25211625 .............................................. 25211626 .............................................. 25211628 .............................................. 25211630 .............................................. 25211631 .............................................. 25211632 .............................................. 25211633 .............................................. 25

219929 .............................................. 13219930 .............................................. 13219931 .............................................. 13219935 .............................................. 17219939 .............................................. 17219940 .............................................. 17219955 .............................................. 26219975 .............................................. 30219979 .............................................. 30220163 .............................................. 14220223 .............................................. 14220283 .............................................. 14220724 .............................................. 16220725 .............................................. 16220726 .............................................. 16220728 .............................................. 16220735 .............................................. 16220736 .............................................. 16220738 .............................................. 16220745 .............................................. 16220746 .............................................. 16220755 .............................................. 16220756 .............................................. 16220775 .............................................. 16220776 .............................................. 16220924 .............................................. 16220925 .............................................. 16220926 .............................................. 16220929 .............................................. 16220935 .............................................. 16220936 .............................................. 16220939 .............................................. 16220945 .............................................. 16220946 .............................................. 16220949 .............................................. 16220955 .............................................. 16220956 .............................................. 16220959 .............................................. 16220975 .............................................. 16220976 .............................................. 16220979 .............................................. 16221014 .............................................. 17221017 .............................................. 17221029 .............................................. 17221131 .............................................. 26221133 .............................................. 26221135 .............................................. 26221140 .............................................. 26221142 .............................................. 26221143 .............................................. 26221144 .............................................. 26221145 .............................................. 26221153 .............................................. 26221154 .............................................. 26221155 .............................................. 26221159 .............................................. 26221163 .............................................. 26221164 .............................................. 26221165 .............................................. 26222333 .............................................. 26223682 .............................................. 89223682-01......................................... 89223683 ...................................... 19, 109223684 ...................................... 19, 109223685 ...................................... 19, 109223686 ...................................... 19, 109223687 ...................................... 19, 109223692 .............................................. 89223692-01......................................... 89223693 ...................................... 19, 109223695 ...................................... 19, 109223696 ...................................... 19, 109223712 ...................................... 19, 109223738 ...................................... 19, 109

196Numeric


223739 ...................................... 19, 109223742 ...................................... 19, 109223743 ...................................... 19, 109223745 ...................................... 19, 109223746 ...................................... 19, 109223747 ...................................... 19, 109223748 ...................................... 19, 109223760 ...................................... 19, 109223761 ...................................... 19, 109223762 ...................................... 19, 109223763 ...................................... 19, 109223764 ...................................... 19, 109223766 ...................................... 19, 109223950 .............................................. 19223952 .............................................. 19224007 .............................................. 19224031 .............................................. 20224033 .............................................. 20224036 .............................................. 20224037 .............................................. 20224080 .............................................. 20224100-020....................................... 34224100-030....................................... 34224100-060....................................... 34224100-070....................................... 34224100-072....................................... 34224100-080............................... 34, 110224100-080..................................... 154224100-081....................................... 34224100-092............................... 34, 110224100-093............................... 34, 110224100-093..................................... 154224100-160....................................... 34224100-161....................................... 34224100-170....................................... 34224100-171....................................... 34224100-172............................... 34, 110 224100-172..................................... 154224100-173....................................... 34224100-174....................................... 34224100-175....................................... 34224100-177....................................... 34224100-178....................................... 34224100-180....................................... 34224100-181................................. 34, 91224100-192............................... 34, 110224100-194............................... 34, 110224100-194..................................... 154224100-202............................... 34, 110224100-202..................................... 154224100-203................................. 18, 34224100-203............................... 42, 108224100-203..................................... 110224100-263....................................... 34224100-282............................... 34, 110224100-320....................................... 34224100-330....................................... 34224100-331....................................... 34224100-342....................................... 34224100-503................................. 13, 15 224100-503................................. 34, 41224100-503............................. 108, 110224100-507................................. 13, 34224100-507............................... 41, 108224100-507..................................... 110224162 .............................................. 33224163 .............................................. 33224168 ........................................ 33, 91224172 .............................................. 33224173 ........................................ 33, 91224174 .............................................. 33224175-01................................. 32, 109224175-05................................. 32, 109224175-06................................. 32, 109

224700 ............................................ 152224701 ............................................ 152224702 ............................................ 152224704 ............................................ 152224705 ............................................ 152224706 ............................................ 152224708 ............................................ 152224710 ............................................ 152224711 ............................................ 152224712 ............................................ 152224714 ............................................ 152224720 ............................................ 151224721 ............................................ 151224722 ............................................ 151224724 ............................................ 151224725 ............................................ 151224726 ............................................ 151224728 ............................................ 151224730 ............................................ 151224731 ............................................ 151224732 ............................................ 151224734 ............................................ 151224740 ............................................ 151224741 ............................................ 151224742 ............................................ 151224744 ............................................ 151224745 ............................................ 151224746 ............................................ 151224748 ............................................ 151224750 ............................................ 151224751 ............................................ 151224752 ............................................ 151224754 ............................................ 151224794 .............................................. 96224794-01......................................... 96224795 .............................................. 96224795-01......................................... 96224801 ............................................ 151224802 ............................................ 151224804 ............................................ 151224805 ............................................ 151224806 ............................................ 151224808 ............................................ 151224811 ............................................ 151224812 ............................................ 151224814 ............................................ 151224821 ............................................ 151224822 ............................................ 151224823 ............................................ 151224831 ............................................ 151224832 ............................................ 151224833 ............................................ 151224836 ............................................ 151224841 ............................................ 153224842 ............................................ 153224881 ............................................ 150224882 ............................................ 150224884 ............................................ 150224885 ............................................ 150224886 ............................................ 150224888 ............................................ 150224891 ............................................ 152224892 ............................................ 152224950 ...................................... 95, 153224952 ...................................... 71, 153224961 ............................................ 153224962 ............................................ 153224981 ............................................ 150224982 ............................................ 150224984 ............................................ 150225002 ............................................ 142225012 ............................................ 150

224175-07................................. 32, 109224176-01................................. 32, 109224176-05................................. 32, 109224176-06................................. 32, 109224176-07................................. 32, 109224177-01................................. 32, 109224177-05................................. 32, 109224177-06................................. 32, 109224178-01................................... 32, 91224178-01....................................... 109224178-05................................. 32, 109224178-06................................. 32, 109224178-07................................. 32, 109224182-01................................. 32, 109224182-05................................. 32, 109224182-06................................. 32, 109224182-07................................. 32, 109224183-01................................... 32, 91224183-01....................................... 109224183-05................................. 32, 109224183-06................................. 32, 109224183-07................................. 32, 109224187-01......................................... 32224189 ...................................... 32, 109224191 ...................................... 32, 109224192-01................................. 32, 109224192-05................................. 32, 109224192-06................................. 32, 109224192-07................................. 32, 109224193-01................................. 32, 109224193-05................................. 32, 109224193-06................................. 32, 109224193-07................................. 32, 109224195 ........................................ 20, 32224196 ........................................ 20, 32224197-01......................................... 32224202 .............................................. 33224203 .............................................. 33224208 .............................................. 33224209-01......................................... 32224211-01................................... 32, 89224211-05................................... 32, 89224211-06................................... 32, 89224211-07................................... 32, 89224218-01......................................... 32224219-01................................... 32, 89224219-05................................... 32, 89224219-06................................... 32, 89224219-07................................... 32, 89224222-01......................................... 32224223-01......................................... 32224231-01................................... 32, 89224231-05................................... 32, 89224231-06................................... 32, 89224231-07................................... 32, 89224234-01......................................... 32224235-01................................... 32, 89224307 .............................................. 35224316 ........................................ 20, 35224321 .............................................. 35224322 .............................................. 35224323 .............................................. 35224357 .............................................. 35224372 .............................................. 36224373 .............................................. 36224381 .............................................. 35224382 .............................................. 35224383 .............................................. 35224465 .............................................. 35224550 .............................................. 35224626 .............................................. 96224627 .............................................. 96224628 .............................................. 96224629 .............................................. 96

225014 ............................................ 150225081 ............................................ 148225120 .............................................. 87225121 .............................................. 87225124 .............................................. 87225126 .............................................. 87225127 .............................................. 87225129 .............................................. 87225130 .............................................. 87225142 ............................................ 142225144 ............................................ 142225145 ............................................ 142225170 .............................................. 95225172 .............................................. 88225173 .............................................. 88225174 .............................................. 88225175 .............................................. 88225179 .............................................. 89225179-01......................................... 89225179-02......................................... 89225179-03......................................... 89225180 .............................................. 93225185 .............................................. 93225190 .............................................. 93225195 .............................................. 93225200 .............................................. 93225205 .............................................. 93225220-01......................................... 92225221-01......................................... 92225235 .............................................. 93225241 .............................................. 87225242 .............................................. 87225255 .................................. 88, 89, 94225256 .............................................. 87225257 .................................. 88, 89, 94225258 .............................................. 96225259 .............................................. 96225260 ........................................ 89, 95225261 .............................................. 89225265 .................................. 88, 89, 94225268 .............................................. 96225277 .............................................. 91225278 .............................................. 91225280 .............................................. 91225288 ............................................ 150225289 ............................................ 150225290 ............................................ 150225310 ............................................ 142225315 ............................................ 142225326 .............................................. 92225328 .............................................. 92225350-531................................. 89, 95225350-631................................. 88, 89225350-631....................................... 94225402 ............................................ 147225414 ............................................ 148225415 ............................................ 148225532 .............................................. 20225534 .............................................. 20225535 .............................................. 20225536 .............................................. 20225538 .............................................. 20225539 .............................................. 20225540 .............................................. 20225541 .............................................. 20225543 .............................................. 20

197


Numeric225544 .............................................. 20225546 .............................................. 20226432 .............................................. 30226433 .............................................. 30226434 .............................................. 30226436 .............................................. 30226732 .............................................. 20226733 .............................................. 20226734 .............................................. 20226772 .............................................. 20226773 .............................................. 20227494-00........................................... 3227494-00G ........................................ 3227494-01........................................... 3227494-01G ........................................ 3227494-02........................................... 3227494-02G ........................................ 3227494-03........................................... 3227494-03G ........................................ 3227494-99........................................... 3227494-99G ........................................ 3227497-00........................................... 3227497-00G ........................................ 3227497-01........................................... 3227497-01G ........................................ 3227497-02........................................... 3227497-02G ........................................ 3227497-03........................................... 3227497-03G ........................................ 3227497-04........................................... 3227497-04G ........................................ 3227497-05........................................... 3227497-05G ........................................ 3227497-06........................................... 3227497-06G ........................................ 3227497-07........................................... 3227497-07G ........................................ 3227497-08........................................... 3227497-08G ........................................ 3227497-09........................................... 3227497-09G ........................................ 3227497-10........................................... 3227497-10G ........................................ 3227497-11........................................... 3227497-11G ........................................ 3227497-12........................................... 3227497-12G ........................................ 3227497-13........................................... 3227497-13G ........................................ 3227497-14........................................... 3227497-14G ........................................ 3227497-15........................................... 3227497-15G ........................................ 3227497-16........................................... 3227497-16G ........................................ 3227497-17........................................... 3227497-17G ........................................ 3227497-18........................................... 3227497-18G ........................................ 3227497-99........................................... 3227497-99G ........................................ 3227498 ................................................ 3227580 ................................................ 4227667 ................................................ 4227670 ................................................ 3227672 ................................................ 3227700 ................................................ 4227723 ................................................ 4227728 ................................................ 4227730 ................................................ 4227735 ................................................ 4228710 ............................................ 153228778 .................................... 149, 153228780 .................................... 149, 153

239403 .............................................. 40239407 .............................................. 40239409 .............................................. 40239412 .............................................. 40239413 .............................................. 40239414 .............................................. 40239415 .............................................. 40239416 .............................................. 40239417 .............................................. 40239418 .............................................. 40239420 .............................................. 40239421 .............................................. 40239422 .............................................. 40239425 .............................................. 40239426 .............................................. 40239427 .............................................. 40239429 .............................................. 40239431 .............................................. 40239433 .............................................. 40239435 .............................................. 40239437 .............................................. 40239438 .............................................. 40239439 .............................................. 40239440 .............................................. 40239442 .............................................. 40239443 .............................................. 40239444 .............................................. 40239445 .............................................. 40239446 .............................................. 40239449 .............................................. 37239450 .............................................. 37239451 .............................................. 37239453 .............................................. 37239455 .............................................. 37239459 .............................................. 37239460 .............................................. 37239473 .............................................. 40239474 .............................................. 40239475 .............................................. 40239477 .............................................. 40239479 .............................................. 40239481 .............................................. 40239483 .............................................. 40239484 .............................................. 40239485 .............................................. 40239486 .............................................. 40239490 .............................................. 40239491 .............................................. 40239492 .............................................. 40239493 .............................................. 40239494 .............................................. 40239501 .............................................. 42239501-02......................................... 42239501-03......................................... 42239501-04......................................... 42239501-06......................................... 42239506 .............................................. 42239506-02......................................... 42239506-03......................................... 42239506-04......................................... 42239506-06......................................... 42239510 .............................................. 42239510-02......................................... 42239510-03......................................... 42239510-04......................................... 42239510-06......................................... 42239512 .............................................. 42239512-02......................................... 42239512-03......................................... 42239512-04......................................... 42239512-06......................................... 42239516 .............................................. 42239516-02......................................... 42239516-03......................................... 42

228783 .................................... 149, 153228785 .................................... 149, 153235239 .............................................. 14237070 ............................................ 137237071 ............................................ 137237072 ............................................ 137239201 .............................................. 40239202 .............................................. 40239203 .............................................. 40239205 ...................................... 8, 9, 40239207 .......................................... 8, 40239209 ...................................... 8, 9, 40239211 ................................ 8, 9, 11, 40239212 ........................................ 7, 8, 9239212 .................................. 10, 11, 40239213 .............................. 7, 10, 11, 40239214 .......................................... 9, 40239215 ........................................ 11, 40239216 ................................ 7, 9, 10, 40239217 .................................. 10, 11, 40239218 ................................ 7, 9, 10, 40239219 .......................................... 9, 40239220 .................................... 7, 10, 40239221 .......................................... 9, 40239222 .................................... 9, 10, 40239225 .............................................. 40239226 .............................................. 40239227 .............................................. 40239229 ...................................... 8, 9, 40239231 .......................................... 8, 40239233 ...................................... 8, 9, 40239235 ................................ 8, 9, 11, 40239236 ........................................ 7, 8, 9239236 .................................. 10, 11, 40239237 .............................. 7, 10, 11, 40239238 .......................................... 9, 40239239 ........................................ 11, 40239240 ................................ 7, 9, 10, 40239241 .................................. 10, 11, 40239242 ................................ 7, 9, 10, 40239243 .......................................... 9, 40239244 .......................................... 7, 10239244 ...................................... 40, 131239245 .......................................... 9, 40239246 .................................... 9, 10, 40239249 .............................................. 37239250 .............................................. 37239251 .............................................. 37239253 ...................................... 8, 9, 37239255 .......................................... 8, 37239257 ...................................... 8, 9, 37239259 ................................ 8, 9, 11, 37239260 ...................................... 8, 9, 10239260 ....................................... 11, 37239273 .............................................. 40239274 .............................................. 40239275 .............................................. 40239277 .............................................. 40239279 .............................................. 40239281 .............................................. 40239283 .............................................. 40239284 .............................................. 40239285 .............................................. 40239286 .............................................. 40239287 .............................................. 40239288 .............................................. 40239289 .............................................. 40239290 .............................................. 40239291 .............................................. 40239292 .............................................. 40239293 .............................................. 40239294 .............................................. 40239401 .............................................. 40239402 .............................................. 40

239516-04......................................... 42239516-06......................................... 42239853 ..................................... 38, 110239853 ............................................ 154240080 .................................. 13, 17, 37240081 ........................................ 13, 37240106 .............................................. 39240112 .............................................. 39240114 .............................................. 39240119 .............................................. 37240121 .............................................. 37240181 .............................................. 37240202 ................................................ 5240203 ................................................ 5240204 ................................................ 5240206 ...................................... 37, 152240208 ...................................... 37, 152240209 ...................................... 37, 110240209 .................................... 152, 154240215 ...................................... 37, 152240216 ...................................... 37, 152240218 ...................................... 37, 152240223 .............................................. 37240228 .............................................. 37240230 .............................................. 39240230-01......................................... 39240231-01......................................... 39240232 .............................................. 39240232-01......................................... 39240234-01......................................... 39240235 .............................................. 39240235-01......................................... 39240240-01......................................... 39240241 .............................................. 39240241-01......................................... 39240242 .............................................. 39240242-01......................................... 39240243 .............................................. 39240243-01......................................... 39240250 .............................................. 39240250-01......................................... 39240251 .............................................. 39240251-01......................................... 39240252 .............................................. 39240252-01......................................... 39240255-01......................................... 39240256 .............................................. 39240256-01......................................... 39240264 .............................................. 37240269 .............................................. 37240278 .............................................. 37240280 .................................. 13, 17, 37240281 ........................................ 13, 37240319 .............................................. 37240321 .............................................. 37240330 .............................................. 37240381 .............................................. 37240408 ...................................... 37, 152240409 ...................................... 37, 152240414 ...................................... 37, 152240415 ...................................... 37, 152240416 ...................................... 37, 152240418 ........................................ 37, 41240418 .................................... 143, 152240419 .............................................. 37240421 .............................................. 37240463 .............................................. 37240480 ........................................ 13, 17240480 ...................................... 37, 130240481 ........................................ 13, 37240481 .................................... 130, 131240555 ................................................ 5240557 ................................................ 5240563 .............................................. 38

198Numeric


240602 .............................................. 95240676-01................................... 18, 42240676-01....................................... 108240676-02................................... 18, 42240676-02...................................... 108240676-03................................... 18, 42240676-03...................................... 108240676-04................................... 18, 42240676-04...................................... 108240676-05................................... 18, 42240676-05....................................... 108240680 ........................................ 13, 38240683 ........................................ 13, 38240706-01................................... 18, 42240706-01....................................... 108240706-02................................... 18, 42240706-02....................................... 108240706-03................................... 18, 42240706-03....................................... 108240706-04................................... 18, 42240706-04....................................... 108240706-05................................... 18, 42240706-05...................................... 108240716-01.................................. 18 , 42240716-01...................................... 108240716-02................................... 18, 42240716-02....................................... 108240716-03................................... 18, 42240716-03....................................... 108240716-04................................... 18, 42240716-04....................................... 108240716-05................................... 18, 42240716-05....................................... 108240726 .................................. 13, 17, 41240726-03................................... 13, 17240726-03........................................ 41240726-04................................... 13, 17240726-04......................................... 41240736 ....................................... 13, 15,240736 ...................................... 41, 108240740 .................................. 13, 15, 41240746 ................................ 13, 41, 108240750 ........................................ 13, 41240754 .............................................. 79240755 .............................................. 79240756 ................................ 13, 41, 108240760 ................................ 13, 41, 108240776 .............................................. 79240780 .............................................. 79240804 ...................................... 38, 148240805 ...................................... 38, 148240817 ...................................... 38, 148240818 ...................................... 38, 148240917 ...................................... 38, 148241009 ...................................... 38, 148241017 ...................................... 38, 148241018 ...................................... 38, 148241317 ...................................... 38, 148242210 .............................................. 40242212 .............................................. 40242214 .............................................. 40242216 .............................................. 40242217 .............................................. 40242218 .............................................. 40242220 .............................................. 40242222 .............................................. 40242224 .............................................. 40242225 .............................................. 40242226 .............................................. 40242228 .............................................. 40242245 .............................................. 95242247 .............................................. 96242401 .............................................. 24242401-01......................................... 24

242534 .............................................. 24242535 .............................................. 24242536 .............................................. 24242537 .............................................. 24242538 .............................................. 24242540 .............................................. 24242564 ............................................ 141242566 ............................................ 141242582 ............................................ 142242584 ............................................ 142242586 ............................................ 142242588 ............................................ 142242590 ............................................ 142242592 ............................................ 142242612 ........................................ 20, 42242615 ........................................ 20, 42242616 ........................................ 20, 42242619 ........................................ 20, 42242760 .............................................. 96242761 .............................................. 96242762 .............................................. 96242765 .............................................. 96242766 .............................................. 96242768 .............................................. 96242772 .............................................. 90242772-01......................................... 90242772-02......................................... 90242772-04......................................... 90242772-05......................................... 90242772-06......................................... 90242775 .............................................. 90242776 .............................................. 90242776-01......................................... 90242776-02......................................... 90242776-04......................................... 90242776-05......................................... 90242782 .............................................. 90242786 .............................................. 90242786-01......................................... 90242786-02......................................... 90242786-04......................................... 90242786-05......................................... 90242800 .............................................. 87242802 .............................................. 87242804 .............................................. 87242806 .............................................. 87242808 .............................................. 87242810 .............................................. 87242815 .............................................. 87264710 .............................................. 15264711 .............................................. 15265318 ........................................ 71, 97265320 ........................................ 71, 97265422 .............................................. 80276840 ...................................... 97, 137276845 .............................................. 97276850 .............................................. 97276860 .............................................. 97277930 .............................................. 97277935 .............................................. 97279040 .............................................. 98279041 .............................................. 98279045 .............................................. 98279050 .............................................. 98281135 .............................................. 68281155 .............................................. 68281158 .............................................. 68293475 .............................................. 40297746 ........................................ 65, 67297749 ........................................ 65, 68297752 ........................................ 65, 67297755 .............................................. 65

242402 .............................................. 24242403 .............................................. 24242404 .............................................. 24242405 .............................................. 24242406 .............................................. 24242407 .............................................. 24242408 .............................................. 24242410 .............................................. 24242411 .............................................. 24242411-01......................................... 24242412 .............................................. 24242413 .............................................. 24242414 .............................................. 24242415 .............................................. 24242416 .............................................. 24242417 .............................................. 24242418 .............................................. 24242420 .............................................. 24242421 .............................................. 24242421-01......................................... 24242422 .............................................. 24242423 .............................................. 24242424 .............................................. 24242425 .............................................. 24242426 .............................................. 24242427 .............................................. 24242428 .............................................. 24242430 .............................................. 24242431 .............................................. 24242431-01......................................... 24242432 .............................................. 24242433 .............................................. 24242434 .............................................. 24242435 .............................................. 24242436 .............................................. 24242437 .............................................. 24242438 .............................................. 24242440 .............................................. 24242501 .............................................. 24242501-01......................................... 24242502 .............................................. 24242503 .............................................. 24242504 .............................................. 24242505 .............................................. 24242506 .............................................. 24242507 .............................................. 24242508 .............................................. 24242509 .............................................. 24242510 .............................................. 24242511 .............................................. 24242511-01......................................... 24242512 .............................................. 24242513 .............................................. 24242514 .............................................. 24242515 .............................................. 24242516 .............................................. 24242517 .............................................. 24242518 .............................................. 24242519 .............................................. 24242520 .............................................. 24242521 .............................................. 24242521-01......................................... 24242522 .............................................. 24242523 .............................................. 24242524 .............................................. 24242525 .............................................. 24242526 .............................................. 24242527 .............................................. 24242528 .............................................. 24242530 .............................................. 24242531 .............................................. 24242531-01......................................... 24242532 .............................................. 24242533 .............................................. 24

297757 .............................................. 65297770 .............................................. 69297771 .............................................. 69297773 .............................................. 69297784-12......................................... 67297800 ........................................ 68, 69297810 .............................................. 69297811 .............................................. 69297814 .............................................. 69297815 .............................................. 69297816 .............................................. 69297825 .............................................. 69297883 .............................................. 69297942 .............................................. 68

300327342 .............................................. 70333927 .............................................. 71333952 .............................................. 72334003 .............................................. 69334014 .............................................. 69334025 .............................................. 69348252 .............................................. 15348253 .............................................. 15348256 .............................................. 15348258 .............................................. 15348272 .............................................. 15348273 .............................................. 15348522 .............................................. 15348524 .............................................. 15348528 .............................................. 15348960-A .......................................... 61348960-B .......................................... 61348961-A .......................................... 61348961-B .......................................... 61348962-A .......................................... 61348962-B .......................................... 61348963-A .......................................... 61348963-B .......................................... 61348964-A .......................................... 61348964-B .......................................... 61348965-A .......................................... 61348965-B .......................................... 61348966-A .......................................... 61348966-B .......................................... 61348967-A .......................................... 61348967-B .......................................... 61348968-A .......................................... 61348968-B .......................................... 61348969 .............................................. 61348970-C .......................................... 61348970-D .......................................... 61348970-F ........................................... 61348970-G .......................................... 61348971-C .......................................... 61348971-D .......................................... 61348971-F ........................................... 61348971-G .......................................... 61348972-C .......................................... 61348972-D .......................................... 61348972-F ........................................... 61348972-G .......................................... 61348973-C .......................................... 61348973-D .......................................... 61348973-F ........................................... 61

199


Numeric348973-G .......................................... 61348974-C .......................................... 61348974-D .......................................... 61348974-F ........................................... 61348974-G .......................................... 61348975-C .......................................... 61348975-D .......................................... 61348975-F ........................................... 61348975-G .......................................... 61348976-C .......................................... 61348976-D .......................................... 61348976-F ........................................... 61348976-G .......................................... 61348977-C .......................................... 61348977-D .......................................... 61348977-F ........................................... 61348977-G .......................................... 61348978-C .......................................... 61348978-D .......................................... 61348978-F ........................................... 61348978-G .......................................... 61348990-A .......................................... 60348990-B .......................................... 60348995-C .......................................... 60348995-D .......................................... 60348995-F ........................................... 60348995-G .......................................... 60349000-A .......................................... 60349000-B .......................................... 60349005-C .......................................... 60349005-D .......................................... 60349005-F ........................................... 60349005-G .......................................... 60349011 .............................................. 60349013 .............................................. 60349014 .............................................. 60349016 .............................................. 60349017 .............................................. 60349018 .............................................. 60351054 .............................................. 49351454 .............................................. 49351456 .............................................. 49351486 .............................................. 49351514 .............................................. 49351516 .............................................. 49353255 .............................................. 49353257 .............................................. 49353259 .............................................. 49353262 .............................................. 49353264 .............................................. 49353266 .............................................. 49354235 .............................................. 49354237 .............................................. 49354239 .............................................. 49354242 .............................................. 49354244 .............................................. 49355392 .............................................. 50355393 .............................................. 50355394 .............................................. 50355395 .............................................. 50355397 .............................................. 50355398 .............................................. 50355442 .............................................. 50355443 .............................................. 50355444 .............................................. 50355445 .............................................. 50355447 .............................................. 50355448 .............................................. 50355752 .............................................. 50355753 .............................................. 50355754 .............................................. 50355755 .............................................. 50355757 .............................................. 50355758 .............................................. 50

356930 .............................................. 52356943 .............................................. 52356945 .............................................. 52356946 .............................................. 52356949 .............................................. 52356952 .............................................. 52356954 .............................................. 52356963 .............................................. 52356965 .............................................. 52356966 .............................................. 52356969 .............................................. 52356972 .............................................. 52356974 .............................................. 52357331 ...................................... 67, 120357335 ............................................ 120357421 .............................................. 46357422 .............................................. 46357424 .............................................. 46357426 .............................................. 46357428 .............................................. 46357535 .............................................. 46357536 .............................................. 46357537 .............................................. 46357538 ........................................ 43, 46357542 .............................................. 43357544 .............................................. 43357546 .............................................. 43357572 .............................................. 44357574 .............................................. 44357576 .............................................. 44357841 .............................................. 45357843 .............................................. 45357844 ........................................ 45, 46357848 .............................................. 46357860 .............................................. 46357966 .............................................. 45357969 .............................................. 45357971 .............................................. 45357974 .............................................. 45357976 .............................................. 45357979 .............................................. 45357981 .............................................. 45357984 .............................................. 45357986 .............................................. 45357989 .............................................. 45357991 .............................................. 45357994 .............................................. 45358003 .............................................. 44358004 .............................................. 44358005 .............................................. 44358006 .............................................. 44358007 .............................................. 44358008 .............................................. 44358009 .............................................. 44358010 .............................................. 44358011 .............................................. 44358012 .............................................. 44358013 .............................................. 44358014 .............................................. 44358026 .............................................. 44358028 ........................................ 44, 45358029 ........................................ 44, 46358031 .............................................. 44358033 ........................................ 44, 45358034 .............................................. 44358036 .............................................. 44358038 ........................................ 44, 45358039 .............................................. 44358041 .............................................. 44358043 ........................................ 44, 45358044 .............................................. 44358046 .............................................. 44358048 ........................................ 44, 45358049 .............................................. 44

355802 .............................................. 50355803 .............................................. 50355804 .............................................. 50355805 .............................................. 50355807 .............................................. 50355808 .............................................. 50356420-A .......................................... 56356420-B .......................................... 56356422-A .......................................... 56356422-B .......................................... 56356424-A .......................................... 56356424-B .......................................... 56356426-C .......................................... 56356426-D .......................................... 56356426-F ........................................... 56356426-G .......................................... 56356428-C .......................................... 56356428-D .......................................... 56356428-F ........................................... 56356428-G .......................................... 56356430-C .......................................... 56356430-D .......................................... 56356430-F ........................................... 56356430-G .......................................... 56356433 .............................................. 56356471 .............................................. 56356830 .............................................. 52356831 .............................................. 52356832 .............................................. 52356834 .............................................. 52356837 .............................................. 52356839 .............................................. 52356841 .............................................. 52356842 .............................................. 52356843 .............................................. 52356844 .............................................. 52356847 .............................................. 52356848 .............................................. 52356849 .............................................. 52356873 .............................................. 51356874 .............................................. 52356875 .............................................. 51356876 .............................................. 51356877 .............................................. 52356878 .............................................. 52356879 .............................................. 51356880 .............................................. 52356882 .............................................. 51356883 .............................................. 52356884 .............................................. 51356885 .............................................. 52356886 .............................................. 52356887 .............................................. 51356888 .............................................. 52356889 .............................................. 51356890 .............................................. 51356891 .............................................. 52356893 .............................................. 52356895 .............................................. 52356896 .............................................. 52356899 .............................................. 52356902 .............................................. 52356904 .............................................. 52356907 .............................................. 52356909 .............................................. 52356910 .............................................. 52356913 .............................................. 52356915 .............................................. 52356916 .............................................. 52356919 .............................................. 52356922 ........................................ 52, 54356924 .............................................. 52356927 .............................................. 52356929 .............................................. 52

358051 .............................................. 44358053 ........................................ 44, 45358054 .............................................. 44358101 .............................................. 45358102 .............................................. 45358103 .............................................. 45358105 .............................................. 45358106 .............................................. 45358107 .............................................. 45358109 .............................................. 45358110 .............................................. 45358111 .............................................. 45358113 .............................................. 45358114 .............................................. 45358115 .............................................. 45358131 .............................................. 45358132 .............................................. 45358133 ........................................ 45, 46358135 .............................................. 45358136 .............................................. 45358137 .............................................. 45358139 .............................................. 45358140 .............................................. 45358141 .............................................. 45358143 .............................................. 45358144 .............................................. 45358145 .............................................. 45358204 .............................................. 46358606 .............................................. 49358607 .............................................. 49358610 .............................................. 49358646 .............................................. 49358647 .............................................. 49358650 .............................................. 49365822 .............................................. 74365824 .............................................. 74365826 .............................................. 74365882 .............................................. 73365884 .............................................. 73365886 .............................................. 73365892 .............................................. 74365894 .............................................. 74365896 .............................................. 74365904 .............................................. 74365906 .............................................. 74365914 .............................................. 74365916 .............................................. 74366012 .............................................. 74366014 .............................................. 74366016 .............................................. 74366022 .............................................. 74366024 .............................................. 74366026 .............................................. 74366042 .............................................. 74366044 .............................................. 74366046 .............................................. 74366074 .............................................. 74366076 .............................................. 74370790 .............................................. 82370792 .............................................. 82374301 ............................................ 127374302 ............................................ 127374304 ............................................ 127374305 ............................................ 127374306 ............................................ 127374308 ............................................ 127374310 ............................................ 127374311 ............................................ 127374313 ............................................ 127374314 ............................................ 127374315 ............................................ 127374316 ............................................ 127374318 ............................................ 127374319 ............................................ 127

200Numeric


374320 ............................................ 128374321 ............................................ 128374322 ............................................ 128374325 ............................................ 128374324 ............................................ 128374326 ............................................ 128374328 ............................................ 128374330 ............................................ 128377160 .............................................. 67377161 .............................................. 67377162 .............................................. 67377163 ........................................ 67, 68377164 ........................................ 67, 68377165 ........................................ 67, 68377166 ........................................ 67, 68377260 .............................................. 68384353 .............................................. 69385772 .............................................. 70385792 .............................................. 72

400415000 .............................................. 69415005 .............................................. 69415010 .............................................. 69415500 .............................................. 69415501 .............................................. 69415502 .............................................. 69416000 .............................................. 69416005 .............................................. 69416010 .............................................. 69419325 ........................................ 75, 76419327 ........................................ 75, 76419330 .............................................. 76419331 .............................................. 76419332 .............................................. 76419333 .............................................. 76419334 .............................................. 76419336 .............................................. 76419337 .............................................. 76419338 .............................................. 76419347 ........................................ 75, 77419350 .................................. 77, 78, 79419352 .............................................. 77419354 .................................. 77, 78, 79419356 ........................................ 77, 80419360 ........................................ 75, 77419362 .............................................. 77419364 .................................. 77, 78, 79419366 .................................. 77, 78, 79419370 ........................................ 75, 77419372 .............................................. 77419374 .............................................. 77419380 ........................................ 75, 78419382 .............................................. 78419385-47................................... 75, 78419390 ........................................ 75, 78419392 .............................................. 78419400-47................................... 75, 78419410 ........................................ 77, 78419415 ........................................ 77, 78419420 ........................................ 77, 78419430 .............................................. 80419450 ........................................ 75, 80419451 ........................................ 75, 80419452 ........................................ 75, 80419455 .............................................. 80419460 ........................................ 75, 80419461 ........................................ 75, 80419462 ........................................ 75, 80419465 .............................................. 80419470 .............................................. 80419471 .............................................. 80419501 .............................................. 79

773900 .............................................. 70774510 .............................................. 70774515 .............................................. 70774600 .............................................. 71774650 .............................................. 72776580 ................................................ 4798550 ............................................ 137

800805000 .............................................. 11805001 .............................................. 11805003 .............................................. 11805007 .............................................. 11805011 .............................................. 11805015 .............................................. 11805020 .............................................. 11805021 .............................................. 11805023 .............................................. 11805027 .............................................. 11805031 .............................................. 11844000 .............................................. 99844002 .............................................. 99844004 .............................................. 99844021 ............................................ 100844022 ............................................ 100844023 ............................................ 100844024 ............................................ 101844025 ............................................ 101844026 ............................................ 101844027 ............................................ 101844029 ............................................ 101844030 ............................................ 101844032 ............................................ 101844035 ............................................ 101844038 ............................................ 101844060 ............................................ 100844062 ............................................ 100844064 ............................................ 100844068 ............................................ 100851160 ............................................ 118851162 ............................................ 118851163 ............................................ 118851164 ............................................ 118851166 ............................................ 118851168 ............................................ 118851172 ............................................ 119851174 ............................................ 119851176 ............................................ 119851178 ............................................ 119851179-01....................................... 119851179-02....................................... 119851180-01....................................... 119851180-02....................................... 119851181-01....................................... 119851181-02....................................... 119851181-03....................................... 119851181-04....................................... 119851183-2......................................... 118851184-2......................................... 118851187-2......................................... 118851188-2......................................... 118851206 ............................................ 113851246 ............................................ 119851247 ............................................ 119851248 ............................................ 119851249 ............................................ 120851250 ............................................ 120851271 ............................................ 119851272 ............................................ 119851276 ............................................ 119851277 ............................................ 119851295 ............................................ 121

419502 .............................................. 79419505 .............................................. 79419506 .............................................. 79419520-47................................... 75, 79419530-47................................... 75, 79419620 .............................................. 78420836 .............................................. 71428126 .............................................. 69428146 .............................................. 69428164 .............................................. 69434320 .............................................. 71438764 .............................................. 70438782 .............................................. 70439195 .............................................. 72439215 .............................................. 72439234 .............................................. 72439264 .............................................. 72

600620189 .............................................. 71635134 ........................................ 49, 69635165 .............................................. 49635229 ........................................ 76, 80635232 ........................................ 77, 80635233 ........................................ 77, 80635245 ........................................ 77, 80635525 ........................................ 78, 80635526 ........................................ 78, 80635527 ........................................ 78, 80635920 .............................................. 98635921 .............................................. 98635926 .............................................. 67635965 .............................................. 67651446 ........................................ 1, 107651462 ................................................ 1651463 ................................................ 1651464 ................................................ 1651466 ................................................ 1651469 ................................................ 1651470 ................................................ 1651482 ................................................ 1651483 ................................................ 1651486 ................................................ 1651490 ............................................ 142651492 ............................................ 142651502 ........................................ 2, 107651506 ........................................ 2, 107651509 ........................................ 2, 107651510 ........................................ 2, 107651905 .................................... 110, 154651907 .................................... 110, 154651954 .................................... 110, 154677670 .............................................. 72690170 .............................................. 70

700709782 .............................................. 68709882 .............................................. 68753680 .............................................. 64753685 .............................................. 64757766 .............................................. 66757769 .............................................. 66757770 .............................................. 66757772 .............................................. 66757773 .............................................. 66757775 .............................................. 66757776 .............................................. 66758966 .............................................. 66758969 .............................................. 66758970 .............................................. 66758972 .............................................. 66758973 .............................................. 66758976 .............................................. 66

851299 ............................................ 121851303 ............................................ 121851307 ............................................ 121851311 ............................................ 121851321 ............................................ 121851322 ............................................ 121851323 ............................................ 121851324 ............................................ 121851325 ............................................ 121851330 ............................................ 121851331 ............................................ 121851332 ............................................ 121851333 ............................................ 121851334 ............................................ 121851340 ............................................ 118851341 ............................................ 120851343 ............................................ 114851344 ............................................ 114851345 ............................................ 118851346 ............................................ 120851355 ............................................ 119851357 ............................................ 119851358 ............................................ 119851371 ............................................ 104851372 ............................................ 104851373 ............................................ 104851375 ............................................ 104851380 .................................... 105, 122851381 ............................................ 105851382 ............................................ 105851383 .................................... 105, 122851388 ............................................ 105851389 ............................................ 105851391 ............................................ 106851392 ............................................ 106851393 ............................................ 106851395 ............................................ 106851401 ............................................ 106851402 ............................................ 106851403 ............................................ 106851405 ............................................ 106851411 ............................................ 105851412 ............................................ 105851414 ............................................ 106851415 ............................................ 106851419 ............................................ 105851421 ............................................ 106851422 ............................................ 106851424 ............................................ 105851430 ............................................ 106851431 ............................................ 106851601 ............................................ 121851604 ............................................ 121851608 ............................................ 121851612 ............................................ 121851616 ............................................ 121851620 ............................................ 121851624 ............................................ 121851625 ............................................ 121868608 ............................................ 149868615 ............................................ 149868617 ............................................ 149868804 ............................................ 149868805 ............................................ 149868806 ............................................ 149868808 ............................................ 149

201


Numeric868810 ............................................ 149885020 ............................................ 130885025 ............................................ 130885200 ............................................ 129885220 ............................................ 129885230 ............................................ 129885231 ............................................ 129885232 ............................................ 129885235 ............................................ 130885236 ............................................ 130885250 ............................................ 130885252 ............................................ 130885253 ............................................ 130885255 ............................................ 130885256 ............................................ 130885257 ............................................ 130885258 ............................................ 130885295 ............................................ 132885300 ............................................ 130885302 ............................................ 130885303 ............................................ 130885380 ............................................ 132885540 ............................................ 132

900900170 ............................................ 136900200 ............................................ 136900201 ............................................ 136900202 ............................................ 136900203 ............................................ 136900204 ............................................ 136900205 ............................................ 136900234 ............................................ 136900254 ............................................ 136900301 ............................................ 136900302 ............................................ 136900303 ............................................ 136900304 ............................................ 136900400 ............................................ 136900401 ............................................ 136900402 ............................................ 136900403 ............................................ 136900404 ............................................ 136900470 ............................................ 137900520 ............................................ 137900522 ............................................ 137900570 ............................................ 137900575 ............................................ 137900620 ............................................ 137900630 ...................................... 97, 136903007 .............................................. 71903061 ...................................... 71, 145903063 ...................................... 71, 145903475 .............................................. 47905275 .............................................. 47905462 .............................................. 47905465 .............................................. 47905475 .............................................. 47923769 .............................................. 70962515 .................................. 69, 71, 72964453 ........................................ 70, 72970440 .............................................. 67985688 .............................................. 91985721 ............................................ 141985726 ............................................ 141985730 ............................................ 141985731 ............................................ 141985734 ............................................ 140985735 ............................................ 140985736 ............................................ 140985737 ............................................ 140985738 ............................................ 140985739 ............................................ 140

I SeriesI050349 ........................................... 101I050567 ............................................. 56I050694 ................................... 126, 127I050916 ........................................... 101I050917 ........................................... 101I051154 ........................................... 101I052665 ............................................. 38I054106 ........................................... 128I054342 ........................................... 127I054343 ........................................... 127I054346 ........................................... 127I054348 ........................................... 127I057152 ............................................. 55I057153 ............................................. 55I057459 ............................................. 54I057501 ............................................. 54I057502 ............................................. 55I057599 ............................................. 54I057606 ............................................. 64I057617 ............................................. 55I057621 ............................................. 55I057622 ............................................. 55I057638 ............................................. 54

W Series W125495 ........................................... 65

W 200 W209677 ........................................... 28W209678 ........................................... 28W209679 ........................................... 28W209900 ........................................... 28W209901 ........................................... 28W209902 ........................................... 28W209903 ........................................... 28W209904 ........................................... 28W209905 ........................................... 28W209906 ........................................... 28W209907 ........................................... 28W209908 ........................................... 28W209909 ........................................... 28W209910 ........................................... 28W209911 ........................................... 28W209912 ........................................... 28W209913 ........................................... 28W209914 ........................................... 28W209915 ........................................... 28W209916 ........................................... 28W209917 ........................................... 28W211523 ............................................. 5W211734 ............................................. 5W211735 ............................................. 5W211754 ............................................. 5W211755 ............................................. 5W215235 ........................................... 14W215255 ........................................... 14W216014 ........................................... 14W216015 ........................................... 14W216800 ............................................. 7W216801 ............................................. 7W216802 ............................................. 7W216803 ............................................. 7W216804 ............................................. 7W216805 ............................................. 7W216806 ............................................. 7W216807 ............................................. 7W216808 ............................................. 7W216809 ............................................. 7W216810 ............................................. 7W216811 ............................................. 7W216812 ............................................. 7W216813 ............................................. 7

985742 ............................................ 141985743 ............................................ 141985744 ............................................ 141985745 ............................................ 140985746 ............................................ 140985747 ............................................ 140985750 ............................................ 149985800 ............................................ 149985810 .................................... 142, 149986294 .............................................. 71986297 .............................................. 71986298 .............................................. 71986299 .............................................. 71986491 ............................................ 148986492 ............................................ 148986532 ............................................ 146986540 ............................................ 146986541 ............................................ 146986542 ............................................ 146986546 ............................................ 146986548 ............................................ 146986560 ............................................ 146986561 ............................................ 146986562 ............................................ 146986568 ............................................ 146986580 ............................................ 146986581 ............................................ 146986582 ............................................ 146986586 ............................................ 146986590 ............................................ 146986591 ............................................ 146986644 ............................................ 148986645 ............................................ 148986700 ............................................ 147986701 ............................................ 147986702 ............................................ 147986704 ............................................ 147986706 ............................................ 147986710 ............................................ 147986711 ............................................ 147986712 ............................................ 147986714 ............................................ 147986716 ............................................ 147986720 ............................................ 147986721 ............................................ 147986722 ............................................ 147986724 ............................................ 147986726 ............................................ 147986730 ............................................ 147986731 ............................................ 147986732 ............................................ 147986734 ............................................ 147986736 ............................................ 147986740 ............................................ 147986741 ............................................ 147986742 ............................................ 147986744 ............................................ 147986746 ............................................ 147986750 ............................................ 147986751 ............................................ 147986752 ............................................ 147986754 ............................................ 147986756 ............................................ 147990250 ............................................ 130990350 ............................................ 131990400 ............................................ 131990450 ............................................ 131990470 ............................................ 131990471 ............................................ 131990476 ............................................ 132990477 .................................... 130, 131990760 .............................................. 82990760-4........................................... 82

W216814 ............................................. 7W216815 ............................................. 7W216816 ............................................. 7W216817 ............................................. 7W216818 ............................................. 7W216819 ............................................. 7W216820 ............................................. 7W216821 ............................................. 7W216822 ............................................. 7W216823 ............................................. 7W216824 ............................................. 7W216825 ............................................. 7W216826 ............................................. 7W216827 ............................................. 7W216828 ............................................. 7W216829 ............................................. 7W216830 ............................................. 7W216831 ............................................. 7W216832 ............................................. 7W216833 ............................................. 7W216834 ............................................. 7W216835 ............................................. 7W216836 ............................................. 8W216837 ............................................. 8W216838 ............................................. 8W216839 ............................................. 8W216840 ............................................. 8W216841 ............................................. 8W216842 ............................................. 8W216843 ............................................. 8W216844 ............................................. 8W216845 ............................................. 8W216846 ............................................. 8W216847 ............................................. 8W216848 ............................................. 8W216849 ............................................. 8W216850 ............................................. 8W216851 ............................................. 8W216852 ............................................. 8W216853 ............................................. 8W216854 ............................................. 8W216855 ............................................. 8W216856 ............................................. 8W216857 ............................................. 8W216858 ............................................. 8W216859 ............................................. 8W216860 ............................................. 8W216861 ............................................. 8W216862 ............................................. 8W216863 ............................................. 8W216864 ............................................. 8W216865 ............................................. 8W216866 ............................................. 8W216867 ............................................. 8W216868 ............................................. 8W216869 ............................................. 8W216870 ............................................. 8W216871 ............................................. 8W216872 ............................................. 8W216873 ............................................. 8W216874 ............................................. 8W216875 ............................................. 8W216876 ............................................. 8W216877 ............................................. 8W216878 ............................................. 8W216879 ............................................. 8W216880 ............................................. 8W216881 ............................................. 8W216882 ............................................. 8W216883 ............................................. 8W216884 ............................................. 8W216885 ............................................. 8W216886 ............................................. 8W216887 ............................................. 8

202Numeric


W216888 ............................................. 8W216889 ............................................. 8W216890 ............................................. 8W216891 ............................................. 8W216892 ............................................. 8W216893 ............................................. 8W216894 ............................................. 8W216895 ............................................. 8W216896 ............................................. 8W216897 ............................................. 8W216898 ............................................. 8W216899 ............................................. 8W216900 ............................................. 8W216901 ............................................. 8W216902 ............................................. 8W216903 ............................................. 9W216904 ............................................. 9W216905 ............................................. 9W216906 ............................................. 9W216907 ............................................. 9W216908 ............................................. 9W216909 ............................................. 9W216910 ............................................. 9W216911 ............................................. 9W216912 ............................................. 9W216913 ............................................. 9W216914 ........................................... 10W216915 ........................................... 10W216916 ........................................... 10W216917 ........................................... 10W216918 ........................................... 10W216919 ........................................... 10W216920 ........................................... 10W216921 ........................................... 10W216922 ........................................... 10W216923 ........................................... 10W216924 ............................................. 9W216925 ............................................. 9W216926 ............................................. 9W216927 ............................................. 9W216928 ............................................. 9W216929 ............................................. 9W216930 ............................................. 9W216931 ............................................. 9W216932 ............................................. 9W216933 ............................................. 9W216934 ............................................. 9W216935 ............................................. 9W216936 ............................................. 9W216937 ............................................. 9W216938 ............................................. 9W216939 ........................................... 11W216940 ........................................... 11W216941 ........................................... 11W216942 ........................................... 11W216943 ........................................... 11W216944 ........................................... 11W216945 ........................................... 11W216946 ........................................... 11W216947 ........................................... 11W216948 ........................................... 11W216949 ........................................... 11W216950 ........................................... 11W216951 ........................................... 11W216952 ........................................... 11W216953 ........................................... 11W216954 ........................................... 11W216955 ........................................... 11W216956 ........................................... 11W216957 ........................................... 11W216958 ........................................... 11W216959 ........................................... 11W216960 ........................................... 11W216961 ........................................... 11

W224100-405 .................................. 110W224100-406 .................................... 33W224100-406 .................................. 110W224100-407 .................................... 33W224100-407 .................................. 110W224100-408 .................................... 33W224100-408 .................................. 110W224100-420 .................................... 33W224100-420 .................................. 110W224100-421 .................................... 33W224100-421 .................................. 110W224215 ........................................... 91W224216 ........................................... 91W224217 ........................................... 91W224581 ......................................... 150W224582 ......................................... 150W224584 ......................................... 150W224585 ......................................... 150W224586 ......................................... 150W224588 ......................................... 150W224589 ......................................... 150W224590 ......................................... 150W224591 ......................................... 150W224600 ................................... 41, 142W224601 ......................................... 143W224603 ......................................... 143W224604 ......................................... 150W224605 ......................................... 150W224606 ......................................... 150W224607 ......................................... 150W224608 ......................................... 150W224609 ......................................... 150W224610 ......................................... 150W224611 ......................................... 150W224612 ......................................... 150W224613 ......................................... 143W224614 ......................................... 150W224617 ......................................... 143W224618 ......................................... 143W224619 ......................................... 143W224620-01 ...................................... 90W224620-0103 .................................. 90W224620-0104 .................................. 90W224620-0105 .................................. 90W224620-0107 .................................. 90W224620-02 ...................................... 90W224620-0203 .................................. 90W224620-0204 .................................. 90W224620-0205 .................................. 90W224620-0207 .................................. 90W224620-03 ...................................... 90W224620-0303 .................................. 90W224620-0304 .................................. 90W224620-0305 .................................. 90W224620-0307 .................................. 90W224621-01 ...................................... 90W224621-0103 .................................. 90W224621-0104 .................................. 90W224621-0105 .................................. 90W224621-0107 .................................. 90W224621-02 ...................................... 90W224621-0203 .................................. 90W224621-0204 .................................. 90W224621-0205 .................................. 90W224621-0207 .................................. 90W224621-03 ...................................... 90W224621-0303 .................................. 90W224621-0304 .................................. 90W224621-0305 .................................. 90W224621-0307 .................................. 90W224622-01 ...................................... 90W224622-0103 .................................. 90

W216962 ........................................... 11W216963 ........................................... 11W216964 ........................................... 11W216965 ........................................... 11W216966 ........................................... 10W216967 ........................................... 10W216968 ........................................... 10W216969 ........................................... 10W216970 ........................................... 10W216971 ........................................... 10W216972 ........................................... 10W216973 ........................................... 10W216974 ........................................... 10W216975 ........................................... 10W216976 ........................................... 10W216977 ........................................... 10W216978 ........................................... 10W216979 ........................................... 10W216980 ........................................... 10W216981 ........................................... 10W216982 ........................................... 10W216983 ........................................... 10W216984 ........................................... 10W216985 ........................................... 10W216986 ........................................... 10W216987 ........................................... 10W216988 ........................................... 10W216989 ........................................... 10W216990 ........................................... 10W216991 ........................................... 10W216992 ........................................... 10W216993 ........................................... 10W216994 ........................................... 10W216995 ............................................. 7W216996 ............................................. 7W216997 ............................................. 7W216998 ............................................. 7W216999 ............................................. 7W217000 ............................................. 7W217001 ............................................. 7W217002 ............................................. 7W217003 ............................................. 7W217004 ............................................. 7W217005 ............................................. 7W217006 ............................................. 7W217007 ............................................. 7W217008 ............................................. 7W217009 ............................................. 7W219937 ........................................... 17W219980 ........................................... 30W219981 ........................................... 22W219982 ........................................... 22W219983 ........................................... 22W219985 ........................................... 22W219986 ........................................... 22W219987 ........................................... 22W219990 ........................................... 22W219991 ........................................... 22W219992 ........................................... 22W219993 ........................................... 22W219994 ........................................... 22W219995 ........................................... 22W219996 ........................................... 22W224100-080 .................................. 154W224100-093 .................................. 154W224100-193 .................................... 34W224100-193 .................................. 110W224100-400 .................................... 33W224100-400 .................................. 110W224100-401 .................................... 33W224100-401 .................................. 110W224100-402 .................................... 33W224100-402 .................................. 110W224100-405 .................................... 33

W224622-0104 .................................. 90W224622-0105 .................................. 90W224622-0107 .................................. 90W224622-02 ...................................... 90W224622-0203 .................................. 90W224622-0204 .................................. 90W224622-0205 .................................. 90W224622-0207 .................................. 90W224622-03 ...................................... 90W224622-0303 .................................. 90W224622-0304 .................................. 90W224622-0305 .................................. 90W224622-0307 .................................. 90W224623-01 ...................................... 90W224623-0103 .................................. 90W224623-0104 .................................. 90W224623-0105 .................................. 90W224623-0107 .................................. 90W224623-02 ...................................... 90W224623-0203 .................................. 90W224623-0204 .................................. 90W224623-0205 .................................. 90W224623-0207 .................................. 90W224623-03 ...................................... 90W224623-0303 .................................. 90W224623-0304 .................................. 90W224623-0305 .................................. 90W224623-0307 .................................. 90W224630 ......................................... 143W224632 ......................................... 143W224634 ......................................... 143W224636 ......................................... 143W224681 ......................................... 150W224682 ......................................... 150W224684 ......................................... 150W224693 ......................................... 153W224694 ......................................... 153W224695 ......................................... 153W224696 ......................................... 153W224697 ......................................... 153W224698 ......................................... 153W224809 ......................................... 152W224810 ......................................... 152W224815 ......................................... 151W224820 ......................................... 150W224840 ......................................... 150W224894 ......................................... 152W224895 ......................................... 152W224896 ......................................... 152W224897 ......................................... 152W224954 ......................................... 153W224955 ......................................... 153W225100 ......................................... 152W225122 ........................................... 87W225123 ........................................... 87W225125 ........................................... 87W225149 ........................................... 94W225150 ........................................... 94W225150-01 ...................................... 94W225150-0101 .................................. 94W225150-0103 .................................. 94W225150-0104 .................................. 94W225150-0105 .................................. 94W225150-0107 .................................. 94W225150-02 ...................................... 94W225150-0201 .................................. 94

203


NumericW225150-0203 .................................. 94W225150-0204 .................................. 94W225150-0205 .................................. 94W225150-0207 .................................. 94W225150-03 ...................................... 94W225150-0301 .................................. 94W225150-06 ...................................... 94W225150-0601 .................................. 94W225150-08 ...................................... 94W225150-0801 .................................. 94W225151 ........................................... 94W225151-01 ...................................... 95W225151-0101 .................................. 95W225151-0103 .................................. 95W225151-0104 .................................. 95W225151-0105 .................................. 95W225151-0107 .................................. 95W225151-02 ...................................... 95W225151-0201 .................................. 95W225151-0203 .................................. 95W225151-0204 .................................. 95W225151-0205 .................................. 95W225151-0207 .................................. 95W225151-03 ...................................... 95W225151-0301 .................................. 95W225151-04 ...................................... 95W225151-0401 .................................. 95W225151-06 ...................................... 95W225151-0601 .................................. 95W225151-08 ...................................... 95W225151-0801 .................................. 95W225152 ........................................... 94W225152-01 ...................................... 95W225152-0101 .................................. 95W225152-0103 .................................. 95W225152-0104 .................................. 95W225152-0105 .................................. 95W225152-0107 .................................. 95W225152-02 ...................................... 95W225152-0201 .................................. 95W225152-0203 .................................. 95W225152-0204 .................................. 95W225152-0205 .................................. 95W225152-0207 .................................. 95W225152-03 ...................................... 95W225152-0301 .................................. 95W225152-06 ...................................... 95W225152-0601 .................................. 95W225152-08 ...................................... 95W225152-0801 .................................. 95W225153 ........................................... 94W225153-01 ...................................... 95W225153-0101 .................................. 95W225153-0103 .................................. 95W225153-0104 .................................. 95W225153-0105 .................................. 95W225153-0107 .................................. 95W225153-02 ...................................... 95W225153-0201 .................................. 95W225153-0203 .................................. 95W225153-0204 .................................. 95W225153-0205 .................................. 95W225153-0207 .................................. 95W225153-03 ...................................... 95W225153-0301 .................................. 95W225153-06 ...................................... 95W225153-0601 .................................. 95W225153-08 ...................................... 95W225153-0801 .................................. 95W225154-04 ...................................... 94W225154-0401 .................................. 94W225156-04 ...................................... 95W225156-0401 .................................. 95W225157-04 ...................................... 95

W225223-0203 .................................. 92W225223-0204 .................................. 92W225223-0207 .................................. 92W225223-03 ...................................... 92W225223-0303 .................................. 92W225223-0304 .................................. 92W225223-0307 .................................. 92W225223-12 ...................................... 92W225223-1203 .................................. 92W225223-1204 .................................. 92W225223-1207 .................................. 92W225225-01 ...................................... 92W225225-0103 .................................. 92W225225-0104 .................................. 92W225225-0107 .................................. 92W225225-02 ...................................... 92W225225-0203 .................................. 92W225225-0204 .................................. 92W225225-0207 .................................. 92W225225-03 ...................................... 92W225225-0303 .................................. 92W225225-0304 .................................. 92W225225-0307 .................................. 92W225225-12 ...................................... 92W225225-1203 .................................. 92W225225-1204 .................................. 92W225225-1207 .................................. 92W225237 ........................................... 93W225243 ........................................... 87W225244 ........................................... 87W225251 ........................................... 87W225278-01 ...................................... 91W225278-02 ...................................... 91W225278-03 ...................................... 91W225280-01 ...................................... 91W225280-02 ...................................... 91W225280-03 ...................................... 91W225291 ......................................... 150W225292 ......................................... 150W225293 ......................................... 150W225300 ..................................... 35, 96W225301 ..................................... 35, 96W225302 ..................................... 35, 96W225303 ..................................... 35, 96W225326-01 ...................................... 92W225326-0101 .................................. 92W225326-0103 .................................. 92W225326-0104 .................................. 92W225326-0105 .................................. 92W225326-0107 .................................. 92W225326-02 ...................................... 92W225326-0201 .................................. 92W225326-0203 .................................. 92W225326-0204 .................................. 92W225326-0205 .................................. 92W225326-0207 .................................. 92W225326-03 ...................................... 92W225326-0301 .................................. 92W225326-04 ...................................... 92W225326-0401 .................................. 92W225326-06 ...................................... 92W225326-0601 .................................. 92W225326-08 ...................................... 92W225326-0801 .................................. 92W225328-01 ...................................... 92W225328-0101 .................................. 92W225328-0103 .................................. 92W225328-0104 .................................. 92W225328-0105 .................................. 92W225328-0107 .................................. 92W225328-02 ...................................... 92W225328-0201 .................................. 92W225328-0203 .................................. 92W225328-0204 .................................. 92

W225157-0401 .................................. 95W225171 ........................................... 94W225172-01 ...................................... 88W225172-0103 .................................. 88W225172-0104 .................................. 88W225172-0107 .................................. 88W225172-02 ...................................... 88W225172-0203 .................................. 88W225172-0204 .................................. 88W225172-0207 .................................. 88W225172-03 ...................................... 88W225172-0303 .................................. 88W225172-0304 .................................. 88W225172-0307 .................................. 88W225172-12 ...................................... 88W225172-1203 .................................. 88W225172-1204 .................................. 88W225172-1207 .................................. 88W225173-01 ...................................... 88W225173-0103 .................................. 88W225173-0104 .................................. 88W225173-0107 .................................. 88W225173-02 ...................................... 88W225173-0203 .................................. 88W225173-0204 .................................. 88W225173-0207 .................................. 88W225173-03 ...................................... 88W225173-0303 .................................. 88W225173-0304 .................................. 88W225173-0307 .................................. 88W225173-12 ...................................... 88W225173-1203 .................................. 88W225173-1204 .................................. 88W225173-1207 .................................. 88W225174-01 ...................................... 88W225174-0103 .................................. 88W225174-0104 .................................. 88W225174-0107 .................................. 88W225174-02 ...................................... 88W225174-0203 .................................. 88W225174-0204 .................................. 88W225174-0207 .................................. 88W225174-03 ...................................... 88W225174-0303 .................................. 88W225174-0304 .................................. 88W225174-0307 .................................. 88W225174-12 ...................................... 88W225174-1203 .................................. 88W225174-1204 .................................. 88W225174-1207 .................................. 88W225175-01 ...................................... 88W225175-0103 .................................. 88W225175-0104 .................................. 88W225175-0107 .................................. 88W225175-02 ...................................... 88W225175-0203 .................................. 88W225175-0204 .................................. 88W225175-0207 .................................. 88W225175-03 ...................................... 88W225175-0303 .................................. 88W225175-0304 .................................. 88W225175-0307 .................................. 88W225175-12 ...................................... 88W225175-1203 .................................. 88W225175-1204 .................................. 88W225175-1207 .................................. 88W225181 ........................................... 93W225186 ........................................... 93W225187 ........................................... 93W225223-01 ...................................... 92W225223-0103 .................................. 92W225223-0104 .................................. 92W225223-0107 .................................. 92W225223-02 ...................................... 92

W225328-0205 .................................. 92W225328-0207 .................................. 92W225328-03 ...................................... 92W225328-0301 .................................. 92W225328-04 ...................................... 92W225328-0401 .................................. 92W225328-06 ...................................... 92W225328-0601 .................................. 92W225328-08 ...................................... 92W225328-0801 .................................. 92W225331-01 ...................................... 94W225331-0101 .................................. 94W225331-0103 .................................. 94W225331-0104 .................................. 94W225331-0105 .................................. 94W225331-0107 .................................. 94W225332-02 ...................................... 94W225332-0201 .................................. 94W225332-0203 .................................. 94W225332-0204 .................................. 94W225332-0205 .................................. 94W225332-0207 .................................. 94W225333-03 ...................................... 94W225333-0301 .................................. 94W225334A-04 ................................... 94W225334A-0401................................ 94W225336-06 ...................................... 94W225336-0601 .................................. 94W225338-08 ...................................... 94W225338-0801 .................................. 94W225350 ..................................... 35, 96W225351 ..................................... 35, 96W225352 ..................................... 35, 96W225353 ..................................... 35, 96W225900 ........................................... 95W225910 ........................................... 95W226060 ......................................... 152W228781 ................................. 149, 153W228782 ................................. 149, 153W239298 ................................... 8, 9, 37W239299 ....................................... 8, 37W239300 ................................... 8, 9, 37W239301 ................................... 8, 9, 37W239302 ................................... 8, 9, 37W239303 ........................................... 37W239304 ....................................... 9. 37W239306 ....................................... 9, 37W239308 ........................................... 37W240382 ........................................... 37W240406 ................................... 37, 152W240506 .................................... 38, 95W240507 ..................................... 38, 95W240508 ........................................... 38W240509 ................................... 38, 110W240509 ........................................ 154W240515 ........................................... 38W240516 ........................................... 38W240517 ........................................... 38W240518 ................................... 38, 142W240540 ........................................... 38W240574 ........................................... 38W240580 ..................................... 38, 95W240581 ..................................... 38, 95W240583 ........................................... 38W240584 ........................................... 38W240585 ........................................... 38W240586 ........................................... 38W240587 ........................................... 38W240588 ........................................... 38W240590 ........................................... 38W240593 ..................................... 38, 68W240594 ........................................... 38W240596 ........................................... 38W240598 ................................... 38, 142

204Numeric


W240599 ........................................... 38W240751 ..................................... 15, 42W240752 ........................................... 42W240820 ..................................... 37, 41W240820 ........................................ 143W240821 ..................................... 37, 41W240821 ......................................... 143W240822 ..................................... 37, 41W240822 ......................................... 143W240823 ..................................... 37, 41W240823 ......................................... 143W240824 ..................................... 37, 41W240824 ......................................... 143W240825 ..................................... 37, 41W240825 ......................................... 143W240827 ..................................... 37, 41W240827 ......................................... 143W240830 ................................... 41, 143W240831 ................................... 41, 143W240832 ................................... 41, 143W240833 ................................... 41, 143W240834 ................................... 41, 143W240835 ................................... 41, 143W240836 ................................... 41, 143W240837 ................................... 41, 143W240840 ................................... 41, 143W240841 ................................... 41, 143W240842 ................................... 41, 143W240843 ................................... 41, 143W240844 ................................... 41, 143W240845 ................................... 41, 143W240846 ................................... 41, 143W240847 ................................... 41, 143W242401-A ....................................... 24W242401-X ........................................ 24W242401-01-A .................................. 24W242401-01-X .................................. 24W242402-A ....................................... 24W242402-X ........................................ 24W242403-A ....................................... 24W242403-X ........................................ 24W242404-A ....................................... 24W242404-X ........................................ 24W242405-A ....................................... 24W242405-X ........................................ 24W242406-A ....................................... 24W242406-X ........................................ 24W242407-A ....................................... 24W242407-X ........................................ 24W242408-A ....................................... 24W242408-X ........................................ 24W242410-A ....................................... 24W242410-X ........................................ 24W242411-A ....................................... 24W242411-X ........................................ 24W242411-01-A .................................. 24W242411-01-X .................................. 24W242412-A ....................................... 24W242412-X ........................................ 24W242413-A ....................................... 24W242413-X ........................................ 24W242414-A ....................................... 24W242414-X ........................................ 24W242415-A ....................................... 24W242415-X ........................................ 24W242416-A ....................................... 24W242416-X ........................................ 24W242417-A ....................................... 24W242417-X ........................................ 24W242418-A ....................................... 24W242418-X ........................................ 24W242420-A ....................................... 24W242420-X ........................................ 24W242421-A ....................................... 24

W242516-A ....................................... 24W242516-X ........................................ 24W242517-A ....................................... 24W242517-X ........................................ 24W242518-A ....................................... 24W242518-X ........................................ 24W242519-A ....................................... 24W242520-A ....................................... 24W242520-X ........................................ 24W242521-A ....................................... 24W242521-X ........................................ 24W242521-01-A .................................. 24W242521-01-X .................................. 24W242522-A ....................................... 24W242522-X ........................................ 24W242523-A ....................................... 24W242523-X ........................................ 24W242524-A ....................................... 24W242524-X ........................................ 24W242525-A ....................................... 24W242525-X ........................................ 24W242526-A ....................................... 24W242526-X ........................................ 24W242527-A ....................................... 24W242527-X ........................................ 24W242528-A ....................................... 24W242528-X ........................................ 24W242530-A ....................................... 24W242530-X ........................................ 24W242531-A ....................................... 24W242531-X ........................................ 24W242531-01-A .................................. 24W242531-01-X .................................. 24W242532-A ....................................... 24W242532-X ........................................ 24W242533-A ....................................... 24W242533-X ........................................ 24W242534-A ....................................... 24W242534-X ........................................ 24W242535-A ....................................... 24W242535-X ........................................ 24W242536-A ....................................... 24W242536-X ........................................ 24W242537-A ....................................... 24W242537-X ........................................ 24W242538-A ....................................... 24W242538-X ........................................ 24W242540-A ....................................... 24W242540-X ........................................ 24W242710 ................................... 41, 143W242711 ................................... 37, 152W242820 ........................................... 23W242820-A ....................................... 23W242820-X ........................................ 23W242821 ........................................... 23W242821-A ....................................... 23W242821-X ........................................ 23W242822 ........................................... 23W242822-A ....................................... 23W242822-X ........................................ 23W242823 ........................................... 23W242823-A ....................................... 23W242823-X ........................................ 23W242824 ........................................... 23W242824-A ....................................... 23W242824-X ........................................ 23W242825 ........................................... 23W242825-A ....................................... 23W242825-X ........................................ 23W242826 ........................................... 23W242826-A ....................................... 23W242826-X ........................................ 23

W242421-X ........................................ 24W242421-01-A .................................. 24W242421-01-X .................................. 24W242421-A ....................................... 24W242421-X ........................................ 24W242422-A ....................................... 24W242422-X ........................................ 24W242423-A ....................................... 24W242423-X ........................................ 24W242424-A ....................................... 24W242424-X ........................................ 24W242425-A ....................................... 24W242425-X ........................................ 24W242426-A ....................................... 24W242426-X ........................................ 24W242427-A ....................................... 24W242427-X ........................................ 24W242428-A ....................................... 24W242428-X ........................................ 24W242430-A ....................................... 24W242430-X ........................................ 24W242431-A ....................................... 24W242431-X ........................................ 24W242431-01-A .................................. 24W242431-01-X .................................. 24W242432-A ....................................... 24W242432-X ........................................ 24W242433-A ....................................... 24W242433-X ........................................ 24W242434-A ....................................... 24W242434-X ........................................ 24W242435-A ....................................... 24W242435-X ........................................ 24W242436-A ....................................... 24W242436-X ........................................ 24W242437-A ....................................... 24W242437-X ........................................ 24W242438-A ....................................... 24W242438-X ........................................ 24W242440-A ....................................... 24W242440-X ........................................ 24W242501-A ....................................... 24W242501-X ........................................ 24W242501-01-A .................................. 24W242501-01-X .................................. 24W242502-A ....................................... 24W242502-X ........................................ 24W242503-A ....................................... 24W242503-X ........................................ 24W242504-A ....................................... 24W242504-X ........................................ 24W242505-A ....................................... 24W242505-X ........................................ 24W242506-A ....................................... 24W242506-X ........................................ 24W242507-A ....................................... 24W242507-X ........................................ 24W242508-A ....................................... 24W242508-X ........................................ 24W242509-A ....................................... 24W242510-A ....................................... 24W242510-X ........................................ 24W242511-A ....................................... 24W242511-X ........................................ 24W242511-01-A .................................. 24W242511-01-X .................................. 24W242512-A ....................................... 24W242512-X ........................................ 24W242513-A ....................................... 24W242513-X ........................................ 24W242514-A ....................................... 24W242514-X ........................................ 24W242515-A ....................................... 24W242515-X ........................................ 24

W242827 ........................................... 23W242827-X ........................................ 23W242828 ........................................... 23W242828-X ........................................ 23W242830 ........................................... 23W242830-A ....................................... 23W242830-X ........................................ 23W242831 ........................................... 23W242831-A ....................................... 23W242831-X ........................................ 23W242832 ........................................... 23W242832-A ....................................... 23W242832-X ........................................ 23W242833 ........................................... 23W242833-A ....................................... 23W242833-X ........................................ 23W242834 ........................................... 23W242834-A ....................................... 23W242834-X ........................................ 23W242835 ........................................... 23W242835-A ....................................... 23W242835-X ........................................ 23W242836 ........................................... 23W242836-A ....................................... 23W242836-X ........................................ 23W242837 ........................................... 23W242837-X ........................................ 23W242838 ........................................... 23W242838-X ........................................ 23W264730 ........................................... 79W297772 ........................................... 68W297774 ........................................... 68W297779 ........................................... 69

W 300 W348880-A ....................................... 62W348880-B ....................................... 62W348880-C ....................................... 62W348880-D ....................................... 62W348880-F ........................................ 62W348880-G ....................................... 62W348880-J ........................................ 62W348881-A ....................................... 62W348881-B ....................................... 62W348881-C ....................................... 62W348881-D ....................................... 62W348881-F ........................................ 62W348881-G ....................................... 62W348881-J ........................................ 62W348882-A ....................................... 62W348882-B ....................................... 62W348882-C ....................................... 62W348882-D ....................................... 62W348882-F ........................................ 62W348882-G ....................................... 62W348882-J ........................................ 62W348884-A ....................................... 62W348884-B ....................................... 62W348884-C ....................................... 62W348884-D ....................................... 62W348884-F ........................................ 62W348884-G ....................................... 62W348884-J ........................................ 62W348885-A ....................................... 62W348885-B ....................................... 62W348885-C ....................................... 62

205


NumericW348885-D ....................................... 62W348885-F ........................................ 62W348885-G ....................................... 62W348885-J ........................................ 62W348886 ........................................... 62W348887 ........................................... 62W348888 ........................................... 62W348889 ........................................... 62W348890 ........................................... 63W348891 ........................................... 63W348898 ........................................... 63W348899 ........................................... 63W348902-A ....................................... 63W348902-B ....................................... 63W348902-C ....................................... 63W348902-D ....................................... 63W348902-F ........................................ 63W348902-G ....................................... 63W348902-J ........................................ 63W348920-CH ..................................... 58W348923-A ....................................... 58W348923-B ....................................... 58W348923-C ....................................... 58W348923-D ....................................... 58W348923-F ........................................ 58W348923-G ....................................... 58W348923-J ........................................ 58W348924-A ....................................... 58W348924-B ....................................... 58W348924-C ....................................... 58W348924-D ....................................... 58W348924-F ........................................ 58W348924-G ....................................... 58W348924-J ........................................ 58W348930-CH ..................................... 58W348970-J ........................................ 61W348971-J ........................................ 61W348972-J ........................................ 61W348973-J ........................................ 61W348974-J ........................................ 61W348975-J ........................................ 61W348976-J ........................................ 61W348977-J ........................................ 61W348978-J ........................................ 61W348995-J ........................................ 60W349005-J ........................................ 60W349020-A ....................................... 63W349020-B ....................................... 63W349020-C ....................................... 63W349020-D ....................................... 63W349020-F ........................................ 63W349020-G ....................................... 63W349020-J ........................................ 63W350000-A ....................................... 54W350000-B ....................................... 54W350000-C ....................................... 54W350000-D ....................................... 54W350000-F ........................................ 54W350000-G ....................................... 54W350000-J ........................................ 54W350010-A ....................................... 54W350010-B ....................................... 54W350010-C ....................................... 54W350010-D ....................................... 54W350010-F ........................................ 54W350010-G ....................................... 54W350010-J ........................................ 54W350020-A ....................................... 54W350020-B ....................................... 54W350020-C ....................................... 54W350020-D ....................................... 54W350020-F ........................................ 54W350020-G ....................................... 54W350020-J ........................................ 54

W810004 ......................................... 113W810006 ......................................... 113W810008 ......................................... 113W810010 ......................................... 113W810012 ......................................... 113W810014 ......................................... 113W810016 ......................................... 113W810018 ......................................... 113W810020 ......................................... 113W810022 ......................................... 113W810024 ......................................... 113W810026 ......................................... 113W810028 ......................................... 113W810030 ......................................... 113W810032 ......................................... 113W810034 ......................................... 113W810036 ......................................... 113W810038 ......................................... 113W810040 ......................................... 113W810042 ......................................... 113W810044 ......................................... 113W810046 ......................................... 113W810048 ......................................... 113W810050 ......................................... 113W810052 ......................................... 113W810054 ......................................... 113W810056 ......................................... 113W810058 ......................................... 113W810060 ......................................... 113W810062 ......................................... 113W810300 ......................................... 112W810302 ......................................... 112W810304 ......................................... 112W810306 ......................................... 112W810308 ......................................... 112W810310 ......................................... 112W810312 ......................................... 112W810314 ......................................... 112W810316 ......................................... 112W810318 ......................................... 112W810319 ......................................... 113W810320 ......................................... 113W810321 ......................................... 113W810323 ......................................... 113W810324 ......................................... 113W810325 ......................................... 113W810326 ......................................... 113W810327 ......................................... 113W810600 ......................................... 112W810602 ......................................... 112W810603 ......................................... 112W810604 ......................................... 112W810606 ......................................... 112W810608 ......................................... 112W810609 ......................................... 112W810610 ......................................... 112W810908-A ..................................... 116W810910-A ..................................... 116W810912-A ..................................... 116W810914-A ..................................... 116W810916-A ..................................... 116W810918-A ..................................... 116W810924-A ..................................... 116W810926-A ..................................... 116W810928-A ..................................... 116W810929-A ..................................... 116W810930-A ..................................... 116W810932-A ..................................... 116W810934-A ..................................... 116W820000 ......................................... 120W820001 ......................................... 120W820002 ......................................... 120W820003 ......................................... 120W820004 ......................................... 120

W350030-A ....................................... 54W350030-B ....................................... 54W350030-C ....................................... 54W350030-D ....................................... 54W350030-F ........................................ 54W350030-G ....................................... 54W350030-J ........................................ 54W350040-A ....................................... 55W350040-B ....................................... 55W350040-C ....................................... 55W350040-D ....................................... 55W350040-F ........................................ 55W350040-G ....................................... 55W350040-J ........................................ 55W350050-A ....................................... 55W350050-B ....................................... 55W350050-C ....................................... 55W350050-D ....................................... 55W350050-F ........................................ 55W350050-G ....................................... 55W350050-J ........................................ 55W350060-A ....................................... 55W350060-B ....................................... 55W350060-C ....................................... 55W350060-D ....................................... 55W350060-F ........................................ 55W350060-G ....................................... 55W350060-J ........................................ 55W350070-A ....................................... 57W350070-B ....................................... 57W350070-C ....................................... 57W350070-D ....................................... 57W350070-F ........................................ 57W350070-G ....................................... 57W350070-J ........................................ 57W356426-J ........................................ 56W356428-J ........................................ 56W356430-J ........................................ 56W356473 ........................................... 56W356700-A ....................................... 56W356700-B ....................................... 56W356700-C ....................................... 56W356700-D ....................................... 56W356700-F ........................................ 56W356700-G ....................................... 56W356700-J ........................................ 56W356805-1........................................ 51W356805-4........................................ 51W375020-A ..................................... 126W375020-B ..................................... 126W375020-C ..................................... 126W375020-D ..................................... 126W375020-F ...................................... 126W375020-G ..................................... 126W375020-J ...................................... 126W375022 ......................................... 126W375023 ......................................... 126W375024 ......................................... 126W375025 ......................................... 126

W 400-700W416788 ........................................... 69W438174 ........................................... 70W651450 ..................................... 1, 107W753684-C-E .................................... 64W753684-D-E .................................... 64W753684-F-E..................................... 64W753684-G-E .................................... 64W753684-J-E ..................................... 64

W 800 W810000 ......................................... 113W810002 ......................................... 113

W820005 ......................................... 120W820006 ......................................... 120W820014 ......................................... 120W820019 ......................................... 116W820020 ......................................... 116W820021 ......................................... 116W820022-A ..................................... 116W820023 ......................................... 120W830000 ......................................... 101W830002 ......................................... 101W830004 ......................................... 101W830006 ......................................... 101W830008 ......................................... 101W830010 ......................................... 101W830012 ......................................... 101W830014 ......................................... 101W830016 ......................................... 101W830018 ......................................... 101W830020 ......................................... 101W830022 ......................................... 101W830024 ......................................... 101W830026 ......................................... 101W830029 ......................................... 101W830030 ......................................... 121W830031 ......................................... 101W830032 ......................................... 101W832000 ......................................... 101W832004 ......................................... 101W832006 ......................................... 101W832008 ......................................... 101W832010 ......................................... 101W832012 ......................................... 101W832014 ......................................... 101W832016 ......................................... 101W832018 ......................................... 101W832020 ......................................... 101W832022 ......................................... 101W832024 ......................................... 101W832026 ......................................... 101W832028 ......................................... 101W832030 ......................................... 101W835000 ......................................... 124W835001 ......................................... 122W835002 ......................................... 122W835004 ......................................... 122W835008 ......................................... 122W835010 ......................................... 121W835012 ......................................... 122W835014 ................................. 105, 122W835016 ................................. 105, 122W835022 ................................. 123, 124W835024 ................................. 123, 124W835026 ................................. 123, 124W835028 ................................. 123, 124W844055 ......................................... 100W844056 ......................................... 100W844057 ......................................... 100W844058 ......................................... 100W844059 ......................................... 100W844061 ......................................... 100W844063 ......................................... 100W844065 ......................................... 100W844066 ......................................... 100W844067 ......................................... 100W845100 ........................................... 47W845101 ........................................... 48W845102 ........................................... 48W845106 ........................................... 47W845107 ........................................... 48W845108 ........................................... 48W845110 ........................................... 47W845112 ........................................... 48W845113 ........................................... 48W845114 ........................................... 48

206Numeric


W845115 ........................................... 48W845116 ........................................... 48W845117 ........................................... 48W845118 ........................................... 48W845119 ........................................... 48W845120 ........................................... 48W845121 ........................................... 48W845122 ........................................... 48W845123 ........................................... 48W845124 ........................................... 48W845125 ........................................... 48W845126 ........................................... 48W845127 ........................................... 48W845130 ........................................... 48W845131 ........................................... 48W845132 ........................................... 48W845133 ........................................... 48W845140 ........................................... 48W845141 ........................................... 48W845142 ........................................... 48W851008 ......................................... 106W851010 ......................................... 106W851011 ......................................... 106W851012 ......................................... 106W851014 ......................................... 106W851016 ......................................... 106W851180-2...................................... 118W851239 ......................................... 120W851239-01 .................................... 119W851239-02 .................................... 119W851239-03 .................................... 119W851342 ......................................... 120W851354 ......................................... 120W851356 ......................................... 120W851359 ......................................... 120W851360 ......................................... 120W851361 ......................................... 120W851362 ......................................... 119W851363 ......................................... 119W851364 ......................................... 120W851365 ......................................... 120W851368 ......................................... 120W851369 ......................................... 120W851369 ......................................... 122W851425 ......................................... 106W851426 ......................................... 106W851427 ......................................... 106W851428 ......................................... 106W851429 ......................................... 106W851434 ......................................... 124W851436 ......................................... 123W851438 ......................................... 123W851444 ......................................... 123W851454 ................................. 123, 124W852000 ......................................... 104W852002 ......................................... 104W852004 ......................................... 104W852006 ......................................... 104W852008 ......................................... 104W852010 ......................................... 104W852012 ......................................... 104W852014 ......................................... 104W852016 ......................................... 104W852018 ......................................... 104W852020 ......................................... 104W853000 ......................................... 104W853002 ......................................... 104W853004 ......................................... 104W853006 ......................................... 104W853008 ......................................... 104W853010 ......................................... 104W853012 ......................................... 104W853014 ......................................... 104W853016 ......................................... 104

W860080 ......................................... 105W860086 ......................................... 105W860088 ......................................... 105W860090 ......................................... 105

W 900 W900180 ......................................... 136W900180-6...................................... 136W900700-A ....................................... 53W900700-B ....................................... 53W900700-C ....................................... 53W900700-D ....................................... 53W900700-F ........................................ 53W900700-G ....................................... 53W900700-J ........................................ 53W900701-A ....................................... 53W900701-B ....................................... 53W900701-C ....................................... 53W900701-D ....................................... 53W900701-F ........................................ 53W900701-G ....................................... 53W900701-J ........................................ 53W900702-A ....................................... 53W900702-B ....................................... 53W900702-C ....................................... 53W900702-D ....................................... 53W900702-F ........................................ 53W900702-G ....................................... 53W900702-J ........................................ 53W900703-A ....................................... 53W900703-B ....................................... 53W900703-C ....................................... 53W900703-D ....................................... 53W900703-F ........................................ 53W900703-G ....................................... 53W900703-J ........................................ 53W900704 ........................................... 53W986211NG .................................... 145W986212NG .................................... 145W986213NG .................................... 145W986214NG .................................... 145W986216NG .................................... 145W986217NG .................................... 145W986219NG .................................... 145W986272NG .................................... 144W986273NG .................................... 144W986274NG .................................... 144W986276NG .................................... 144W986277NG .................................... 144W986279NG .................................... 144W986253NG .................................... 144W986254NG .................................... 144W986256NG .................................... 144W986257NG .................................... 144W986259NG .................................... 144W986260NG .................................... 144W986261NG .................................... 144W986281NG .................................... 144W986282NG .................................... 144W986283NG .................................... 144W986284NG .................................... 144W986287NG .................................... 144W986288NG .................................... 144W986289NG .................................... 144W986290NG .................................... 144W986293NG .................................... 144W986294NG .................................... 144W986297NG .................................... 144W986298NG .................................... 144W986299NG .................................... 144W986313NG .................................... 145W986314NG .................................... 145

W853018 ......................................... 104W853020 ......................................... 104W855000 ......................................... 105W855002 ......................................... 105W855004 ......................................... 105W855006 ......................................... 105W855010 ......................................... 105W855012 ......................................... 105W855014 ......................................... 105W855016 ......................................... 105W855018 ......................................... 105W855020 ......................................... 105W855022 ......................................... 105W855024 ......................................... 105W860000 ......................................... 105W860001 ......................................... 105W860002 ......................................... 105W860003 ......................................... 105W860004 ......................................... 105W860005 ......................................... 105W860006 ......................................... 105W860007 ......................................... 105W860008 ......................................... 105W860009 ......................................... 105W860010 ......................................... 105W860011 ......................................... 105W860012 ......................................... 105W860013 ......................................... 105W860014 ......................................... 105W860015 ......................................... 105W860016 ......................................... 105W860017 ......................................... 105W860018 ......................................... 105W860019 ......................................... 105W860020 ......................................... 105W860021 ......................................... 105W860022 ......................................... 105W860023 ......................................... 105W860024 ......................................... 105W860025 ......................................... 105W860026 ......................................... 105W860027 ......................................... 105W860028 ......................................... 105W860029 ......................................... 105W860030 ......................................... 105W860031 ......................................... 105W860032 ......................................... 105W860033 ......................................... 105W860034 ......................................... 105W860035 ......................................... 105W860036 ......................................... 105W860037 ......................................... 105W860038 ......................................... 105W860039 ......................................... 105W860040 ......................................... 105W860041 ......................................... 105W860042 ......................................... 105W860043 ......................................... 105W860044 ......................................... 105W860048 ......................................... 106W860049 ......................................... 106W860051 ......................................... 106W860052 ......................................... 106W860054 ......................................... 106W860056 ......................................... 106W860058 ......................................... 106W860060 ......................................... 106W860062 ......................................... 106W860064 ......................................... 106W860066 ......................................... 106W860068 ......................................... 105W860072 ......................................... 105W860074 ......................................... 105W860078 ......................................... 105

W986316NG .................................... 145W986317NG .................................... 145W986319NG .................................... 145W986333NG .................................... 145W986334NG .................................... 145W986336NG .................................... 145W986337NG .................................... 145W986339NG .................................... 145W986353NG .................................... 145W986354NG .................................... 145W986356NG .................................... 145W986357NG .................................... 145W986359NG .................................... 145W990800 ........................................... 81

WF SeriesWF030089 ......................................... 80WF033112 ......................................... 71WF034088 ......................................... 70WF034093 ......................................... 70WF034590 ......................................... 79

WI SeriesWI050950 ........................................ 123WI055902 .......................................... 54WI055974 .......................................... 55WI055975 .......................................... 55WI056028 .......................................... 64WI057606-C-E ................................... 64WI057606-D-E ................................... 64WI057606-J-E .................................... 64

WL Series WL052594 ......................................... 56WL054376 ......................................... 54WL054377 ......................................... 54WL055973 ......................................... 56

207


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Customized Services

Code 39Code 128Interleaved 2 of 5Linear Bar Codes2 Dimensional (2D)Data Matrix

Laser: Direct product markedCeramic: Imaged ceramic label applied and firedPhoto Imaging: Ultra high-density graphically

perfect imagesFlexographic: Ink based static printing and label

convertingDigital: Full process color 1800 dpi, variable

data

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W hile our heritage is in manufacturing quality products, today we provide services that streamline your operations. It’s easier than ever to get your laboratory products customized the way you need them.

We have integrated a suite of services that you can select from to ensure you obtain the right solution. Each customer is different and each project unique-so each solution is modified to meet individual needs and requirements. Quality products are what we are known for and now with SciLutions®, we bring a whole new level of services to your doorstep.

Made to orderEngineering and design support Custom packaging Custom cut glass tubing and rodsArtwork/decal application

Request a specific packaging method or a completely unique container based on your specific requirements for dimension, shape, and material. Need a custom glass vial? We’ve been producing specialty vials like V Vials® and the E-Z Ex-Traction® high recovery vials for years following the precise instructions of our customers.

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Uniquely numbered and bar coded to fit your application100% scannableWide range of symbologiesPackaged and sequentially numbered to your specifications

Bar coding can help you identify and track anything in your lab or compound storage facility. Increase the speed of data collection while eliminating errors and ultimately reducing costs. It is imperative that bar codes are read correctly every time, stay adhered to the container, and are durable enough to withstand severe environments.

We utilize a variety of symbologies and materials to provide unique identification solutions for biotech, pharmaceutical, and compound storage facilities. Bar coding techniques are available from simple pressure sensitive labels to direct marked containers.

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Extrusion Blow MoldingInjection Blow MoldingInjection MoldingInjection Stretch Blow MoldingTubular Vial Conversion

Molded Glass

High Density Polyethylene (HDPE)Low Density Polyethylene (LDPE)Polyethylene Terephthalate (PET)Polypropylene (PP)Cyclo-Olefin Copolymer (COC)Type I Borosilicate GlassType IIl Soda-Lime Glass

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When needed, an expert team of corporate engineers, R&D professionals and integration specialists will work collaboratively with you to achieve a solution that meets your needs.

We take care of the details. We will handle all of the in-between steps to ensure your process is smooth and efficient. Most of all, we want to meet your particular requirements and specifications from the beginning to the end.

SciLutions is about simplifying your supply chain and optimizing your operations. We can work collaboratively on long term solutions and ensure they are achieved. It’s not about one order or one delivery. It’s about ensuring our products arrive ready to be used, exactly the way you want them.

210Customized Services


Pre-weighed containersTraceability documentationAccuracy to ±0.2 milligrams

Part of the SciLutions® program is to provide you with pre-weighed containers. Taring ensures that the amount of material in the container can be accurately measured at any given time. Wheaton offers this service, eliminating the extra step of pre-weighing in the lab. Weights for each container are recorded to the accuracy of ±0.2 milligrams. The results are summarized in the form of a spreadsheet allowing for each and every container to be fully traceable.

•••

Trace organic analysisStorage of materials prone to adhering to glassExtraction glassware

Surface treatment refers to a process by which the inside of a container is deactivated via silanization or siliconization. The coating provides a protective barrier between

the contents and the glass, eliminating active sites on the glass that could potentially react with its contents.

We treat vials and vial inserts using vapor phase deposition for silanization and siliconization coating to lubricate and protect against alkalinization. This service is ideal for serum vials and pH sensitive storage. Certificates of conformance are

always available based on customer needs and requirements.

•••

Low particulate cleaningDepyrogenationChemical cleaning for trace analysisSterilization (E-beam, Gamma Irradiation, Autoclaving)Water for Injection (WFI) and USP rinsesTotal Organic Carbon (TOC) process

Depending on your application, you may require any number of “levels of clean.” To ensure we can accommodate any request, we employ the most sophisticated methods to remove trace organics, trace inorganics, volatile organics, and organic carbon residues.

We use USP purified water, HEPA filtering, and package items in certified class 100/10 cleanrooms. In addition, we can deliver our glass containers and accessories with endotoxin levels of less than 0.06 EU/mL with full documentation and certification.

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Certificate of Analysis, TOCCertificate of Analysis, EndotoxinCertificate of Analysis, ParticlesCertificate of ProcessingCertificate of Sterility/USPCertification of ComplianceSelected Testing by USP MethodsMaterials Certification

Individual or multi-unit cleanroom bagsSingle or double baggedAutoclaved bagging in cleanroom bags

(closures only)Autoclaved bagging

(single, double, or triple bagged)

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The following are U.S. registered trademarks owned by WHEATON Industries Inc.:

Biopro Computer software BioStir Heavy duty magnetic stirrer C-Tubes Disposable culture tubes CART Computer Aided Roller Technology Cell Optimizer System Large scale cell production system for the culture of mammalian or insect cells Celstir Suspension culture flask Clear-Seal ST Joint that seals without grease Crimpmaster Crimping station Cryule Cryogenic ampule Dura-Grind Stainless steel tissue grinder E-Z Ex-Traction High recovery vials E-Z Sampler Water sampler E-Z Vial Large opening chromatography vial Grab Sampler Water sampler Magna Flex Microcarrier flask MantaRay Single use cell culture vessel Micro-Stir Slow speed stirrer for microcarrier flask Mini-File Mini filing system for sample and micro product vials M-T Vial File Mini filing system for vials Omnispense Automatic dispenser for low-medium viscosity fluids Omni-Vial Disposable scintillation vial Safe-Grind Plastic coated tissue grinder Sampule Disposable 6 mL vial SciLutions Value added services Shorty Vial Vial with low profile The Wheaton Connection Screw thread connection Uni-Dose Disposable container for individual medication Unispense Automatic Dispenser V Vial Vial with cone shaped interior Vacule Cryogenic ampule Vial File Container with alpha-numeric index for filing vials Wheaton Dry Seal Desiccator that seals without grease Wheaton Micro Kit Micro glassware with Wheaton Connection Wheaton Industries Stylized W

The following are trademarks owned by WHEATON Industries Inc.:

ABC Vial Screw top vials for chromatography analysis Bioware Assorted biotechnology products Control Tower Digital control system E-Z Crimper Ergonomic designed hand crimper E-Z Decapper Ergonomic designed hand decapper E-Z Seal Aluminum seals with a larger target diameter I-Loc Closure for diagnostic applications LVI Chromatography vial with a sealed-in limited volume insert Lab 45 Borosilicate media bottles with 45 mm screw thread finish Lab File Carton with drawers for filing Mini Pilot Plant Spinner flask system NextGen V vials with a non-ground base Overdrive Large volume cell culture system Proven Tools R2

P Roller culture apparatus Valu-Bulk Large quantity of containers packed economically Valumetric Graduated bottles Wheaton Bench Top Roller Bench top roller Wheaton Media Square PETE Square media bottles Wheaton Semi-Micro Kit Semi-micro glassware with Wheaton Connection Wheaton S/T Bottle with screw thread finish

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The following trademarks are owned by third parties and may be used in Wheaton Industries Inc. publications:

Acura® Socorex ISBA Acurex® Socorex ISBA Calibra® Socorex ISBA Calibrex® Socorex ISBA Dosys™ Socorex ISBA Ecostep™ Socorex ISBA Excel® Microsoft Corporation F217® Tri-Seal International Inc. Gold Band® Alcan Gore-Tex® W.L. Gore & Associates JusTip™ Socorex ISBA Microlink® St. Gobain Microsep® St. Gobain Microsoft® Microsoft Corporation Microtiter® Thermo Scientific Multi-Tier®* J.G. Finneran Associates, Inc. Poly Crimp®* J.G. Finneran Associates, Inc. Poly Seal® Berry Plastics Corporation Pyrex® Corning Incorporated Profiller™ Socorex ISBA Rulon® St. Gobain Qualitips™ Socorex ISBA Solid Calibration™ Socorex ISBA Snap Seal®* J.G. Finneran Associates Inc. Snap Top Cap® J.G. Finneran Associates, Inc. Step-Pette® Socorex ISBA Stomacher® Seward Limited, Worthing, UK SureSeal® J.S. Plastics Inc. Stepper™ Socorex ISBA Teflon® E.I. duPont de Nemours Topas® Ticona GmbH TPX® Imperial Chemical Industries, Ltd. Tygon® Norton Company Viton® DuPont Dow Elastomers, LLC Waters® Waters Investment Limited Windows® Microsoft Corporation WISP® Millipore Corporation Zitex® Norton Company

* Patented

Our Customers, Associates and Community

As a means of meeting or exceeding our business goals, we focus our Continuous Improvement resources on initiatives that provide maximum value for all stakeholders.

Additionally, we seek to embed a Continuous Improvement mindset in our culture and personal behaviors through extensive participation of our associates, use of proven training, tools and techniques, and the celebration of our successes.

We will supply products and services that consistently meet or exceed our customers’ expectations and requirements, on time, every time. Through continuous improvements, communications, and measurable quality objectives we will continuously strive for excellence in everything we do.

Through the personal involvement of all our associates, we will pursue these commitments to achieve profitable growth for our stakeholders.

• IS

O

9001 & 14001 •Registered

OHS A S 1 8001

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Wheaton Industries is committed to excellence in Environment, Health, and Safety (EHS). As a socially responsible corporate citizen, we will conduct our business in accordance with all applicable legal standards and strive to meet the highest ethical standards. We shall serve to promote a safe and healthy environment, while seeking to improve the quality of life for our associates, contractors, visitors, and the community. This commitment is supported at all levels of the organization and is the individual and collective responsibility of all Wheaton associates.

Promote a positive “Safety First” lifestyle both at work and at home.

Provide education and training to ensure that each associate is experienced and competent to perform their duties safely.

Apply our EHS policies and procedures to all contractors, vendors, and suppliers.

Identify and eliminate foreseeable workplace hazards in our pursuit of zero injuries.

Meet or exceed all EHS regulatory requirements and applicable laws.

Reduce environmental impacts and conserve natural resources.

Integrate EHS as an essential part of our every day business.

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Wheaton Industries is taking initiatives towards protecting our environment. As a company, we have implemented several programs to remove hazardous material from our warehouses and manufacturing plants, reduce our waste, reduce our pollution output, and recycle our aluminum and plastics.

Wheaton Industries Inc. is committed to supporting the WEEE and RoHS Directives. For specific information regarding our policies, please visit our web site, www.wheatonsci.com.

•

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Through our EHS management system and continuous improvement initiatives we shall:

213


We maintain a worldwide network of distributors through which you can purchase the products shown in this catalog. For our complete distributor list, or for a distributor near you, please visit our website at www.wheatonsci.com. Our suggested list prices for our products are listed on our website in U.S. Dollars. When placing an order, please be ready to provide the Wheaton catalog number and product description to the distributor’s Customer Service Representative.

The product information and specifications shown in this catalog were in effect at the time of publication, but are subject to change without notice. For current information please visit our website or contact our customer service department with requests for products which differ from those shown in this catalog. We have a wide range of manufacturing capabilities, and we would be glad to discuss a solution for your business need.

NOTE: WHEATON INDUSTRIES INC. RESERVES THE RIGHT TO MAKE SPECIFICATION CHANGES TO OUR CATALOG PRODUCTS WITHOUT NOTICE. AS A RESULT OF CONTINUAL IMPROVEMENTS TO THE DESIGN OF OUR PRODUCTS OR MANUFACTURING TECHNIQUES, PURCHASERS MAY NOTICE THAT ITEMS ORDERED AND RECEIVED MAY DIFFER FROM THE DESCRIPTION AND/OR PHOTOGRAPH OF THAT PRODUCT APPEARING IN THIS CATALOG.

We are prohibited from shipping any of our products to purchasers in countries listed on the United States embargo list. For a list of these countries please refer to the U.S. Department of Commerce website.

All requests for exchanges or returns are to be made within 30 days of receipt of shipment and should be directed to the distributor through which you placed your order. All exchanges or returns must have prior approval and be sent back in their original packaging. Returned products will not be accepted without prior approval, an authorized RGA number and original packaging. With respect to returns due to incorrect orders, freight charges must be prepaid and a 25% restocking fee will be assessed against the invoiced amount. NOTE: ROLLER CULTURE APPARATUS AND BIOREACTORS ARE MADE TO ORDER AND MAY NOT BE RETURNED.

All shipments should be opened immediately upon receipt for inspection for concealed damages. If concealed damage is discovered, hold the shipping container and packaging materials and immediately request that the carrier make an inspection. If you find damage or shortages, do not accept the shipment until the carrier makes a notation on your delivery receipt or freight bill. All requests for inspection must be made to the carrier within 15 days of receipt of shipment. All claims for damages must be made against the carrier.

All goods and materials shall conform to Wheaton Industries Inc. specifications at the time of shipment from our plant. At our option, we will either correct the non-conforming condition or replace any nonconforming goods or materials or issue you a credit in the amount of the purchase price paid and received for such goods. You must notify us of any claim of nonconformity within ten (10) days after you discover any such defect. Before any claim of nonconformity will be honored, we must be given an opportunity, after receiving notice of your claim, to inspect the goods or materials claimed to be nonconforming. In no event shall any claim for a breach of warranty be made later than one (1) year after the date of delivery of the goods and materials.

WE MAKE NO OTHER EXPRESS OR IMPLIED WARRANTY, STATUTORY OR OTHERWISE, CONCERNING MATERIALS OR GOODS SUPPLIED, INCLUDING WITHOUT LIMITATION, ANY WARRANTY OF FITNESS FOR A PARTICULAR PURPOSE OR ANY WARRANTY OF MERCHANTABILITY. THE WARRANTIES GIVEN HEREUNDER ARE EXCLUSIVELY IN LIEU OF ALL OTHER WARRANTIES EXPRESS OR IMPLIED. WE SHALL NOT BE LIABLE FOR CONSEQUENTIAL, SPECIAL OR INCIDENTAL DAMAGES.

WE MAKE NO WARRANTY, EXPRESS OR IMPLIED, WITH RESPECT TO DEFECTS IN OR NONCONFORMITY OF GOODS OR MATERIALS RESULTING FROM DESIGNS OR SPECIFICATIONS PROVIDED BY YOU. TECHNICAL ADVICE IS FURNISHED AS AN ACCOMMODATION TO YOU. WE ASSUME NO LIABILITY FOR TECHNICAL ADVICE AND YOU ACCEPT SUCH ADVICE AT YOUR SOLE RISK.

Copyright 2008 Wheaton Industries Inc. All rights reserved.

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Container Materials Cap Liner Materials Cap Materials Septa and Stopper Materials Poly- Butyl Natural Chemical COC Glass HDPE LDPE PET PP Al Foil LDPE Vinyl SBR Silicone Teflon PBT Phenolic PP Urea Rubber EPDM Rubber Silicone Teflon Viton

acetic acid, glacial A A A B A A A B B C B A C A A D B B B B A A

acetone A A D D C B A D D D B A D A B A B A B B A D

acetonitrile — A A A B A A A D B D A — A A — D B D D A D

acrylonitrile — A A A B B B A D C D A — D B — D A D D A D

ammonium sulfide — A A A — A D A A B A A — A A C A A A A A C

benzene D A D D C D B D D D D A A A D A D D D D A A

bleach — A A B C B D B A D B A C D B — A A D B A A

boric acid — A A A A A D A A A A A A B A — A A A A A A

carbonic acid — A A A — A B A A B A A — — A — A A A A A A

chlorobenzene D A C D B C A D D D D A B A C B D D D D A A

chloroform D A C C D D A C D D D A D A D A D D D D A A

dichloromethane D A C D D C D D D D D A D C C B D D D D A B

diethylamine — A C D — B A D D B B A — — B — B B B B A C

dimethyl formamide — A A A B A A A D D B A — A A — D B D B A D

dimethyl sulfoxide (DMSO) — A A A B A A A D D D A — — A — D A D D A D

dioxane — A B B A D D B D D D A B A D — B B D D A D

ether — A C D A D B D D D D A — B D B D C D D A C

ethyl acetate C A B B B C B B D D C A C A C B C C D C A D

ethyl alcohol A A A A A A B A B A B A A B A A A A A B A A

ethylene glycol A A A A A A B A A A A A A B A B A A A A A A

formaldehyde — A A A B A A A C B B A — B A A A A C B A C

formic acid 50% A A A B — A C B B B C A A C A D A A B C A C

gasoline D A C D B C A D D D D A A B C A D D D D A A

glycerine A A A A — A A A C A B A — A A — A A A B A A

heptane D A C D B C A D C D D A A A C A D D D D A A

hexane D A B D C B A D D D D A A B B — D D D D A A

hydrochloric acid (HCL) 50% A A A A B A D A B D D A C A A D A D D D A A

hydrofluoric acid (HF) 50% D D A A C A D A C D D A C D A D C D C D A A

hydrogen peroxide 50% — B A A B A A A C C B A B D A D B B B B A A

iodine B A C D A C A D C B A A — — C — B B D A A A

isopropyl alcohol A A A A A A A A B B A A A A A — A A A A A A

methyl alcohol A A A A B A A A C A A A B B A A A A A A A D

methyl ethyl ketone (MEK) — A D D B B A D D D D A C A B — A B D D A D

methylene chloride D A C D D B D D D D D A D C B B D D D D A B

nitric acid 50% B A C B C C D B B D D A C B C D C D C D A B

pentane D A C C — D A C D D D A — — D — D D D D A A

perchloric acid 50% — B B B B B D B D D D B — — B — B B D D B A

phenol 50% B A D D D D A D C D D A D A D — D C D D A A

phosphoric acid 50% A A A A B A B A B D D A B B A D B B D D A A

picric acid — A D D B D A D D B D A — A D D B B B D A A

potassium hydroxide A D A A D A D A A B C A C D A — A A B C A B

sodium hydroxide 50% B D A B D A D B C A B A C D A C A A A B A B

sodium peroxide — A B B — B C B A B D A — B B D A A B D A A

sodium thiosulfate — A A A B A A A A B A A — A A B A A B A A A

sulfuric acid 50% A A A A B B C A C D D A B C B D D C D D A A

tetrahydrofuran (THF) — A C C A B A C D D D A D A B — C C D D A D

toluene D A C C C C A C C D D A D A C — D D D D A B

trifluroacetic acid (TFA) 50% — A A A B A — A A — D — — — A — — A — D — —

vegetable oil — A B B A A A B A D A A A A A A C C D A A A

xylene D A C D C D A D D D D A C A D B D D D D A A

(Tests conducted at room temp) A - Resistant B - Limited Resistance C - Poor Resistance D - Not Resistant — - UnknownCOC ...cyclic olefin copolymer HDPE ... high density polyethylene LDPE ... low density polyethylene PET ... poyethylene terephthalate PP ... polypropylene Al Foil ... aluminum foil SBR ...styrene butadiene rubber Teflon ... (tetrafluoroethylene) PBT ... polybutylene terephthalate Urea ... urea EPDM ... ethylene propylene diene monomer Viton ... (fluoroelastomer)

Although the information in this chart was acquired from reputable sources, it should only be used as a guide in selecting a container and closure system. Because so many factors can affect the chemical resis-tance of a material, in-house testing under actual conditions should be performed. Wheaton Science Products accepts no responsibility for the accuracy of this data or for any consequences resulting from its use.

1501 North 10th Street, Millville, NJ 08332-20381.800.225.1437 (U.S. & Canada) ■ 1.856.825.1368 (F)

1501 North 10th Street, Millville, NJ 08332-2038 USA+1.856.825.1100 ■ +1.856.825.1368 (F)

www.wheatonsci.com (Worldwide)

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