What is Immunofluorescence?

• Technique allowing the visualization of aspecific protein or antigen in cells or tissuesections by binding a specific antibodychemically conjugated with a fluorescent dye

• Immunofluorescence staining can beperformed on cells fixed on slides and tissuesections.

• Immunofluorescence stained samples areexamined under a fluorescence microscopeor confocal microscope.

Source: http://www.protocol-online.org/prot/Immunology/Immunofluorescence/

Sample Image

Immunofluorescence of Anti-nuclear antibody

Source: http://clinicalchemistry.stanford.edu/allergy_autoimmune.html