What are Micropipettors?!We will be using 3

different sizes of micropipettors:◦ P20◦ P200◦ P1000

Very expensive and very fragile – USE Responsibly!!!

Used to measure liquid amounts LESS THAN or EQUAL to 1 mL.

When to Use Each Micropipettor

Each micropipettor has its name on the top cap.

Each micropipette has a limit:◦P20: 0.5 to 20.0 uL◦P200: 20 to 200 uL◦P1000: 200 to 1000 uL

How to Set/Adjust the MicropipettorAdjustment knobs are on

the side of each micropipettor.

Red digits indicate a decimal place

How to Load and Dispense LiquidsPlace clean plastic tip at end of

micropipettorPlunger has TWO different ‘stop’

positions.◦First is used to load (pick up) liquid◦The second (lower) is used to

dispense (expel) liquidTwo stop method is what creates

accuracy and precision of instrument – YOU MUST BE CONSISTENT

Micropipettor Use - DOsAlways use the correct

micropipettor for the volume being measured

Always use the correct pipette tip for each size micropipettor

Use the first stop for loadingUse the second stop for

dispensingRelease plunger slowly

Micropipettor Use - DON’Tshttp://www.youtube.com/watch?v

=1J3w4cS2MvMNO Double Dipping

◦When in doubt – CHANGE TIPS!!Don’t place tip into liquid too

deeply.Use the two stops appropriatelyDon’t squirt liquid out too quicklyDon’t point tip up – this will

contaminate the barrel