Welcome to Biotechnology1st/8th Period – Mr. Young

Please pick up the materials on the table,

look around if you’d like, and find a seat

What you will hopefully gain from this Biotechnology:

•A general knowledge of the goals and functions of the biotechnology industry — “The Big Picture.”

•Experience with the processes used in the biotechnology industry, including experimental design, use of scientific equipment, data analysis and interpretation, critical thinking, and communication.

•The development of good business practices, organizational skills, and self-reliance.

What you will hopefully gain from this Biotechnology:

•Consider this a technical training course

What you will hopefully gain from this Biotechnology:

Who is Matt Young•8th year teacher, 7th year at Consol•Graduated A&M with a B.S. in Biology

in 2006•Member of Search Dog Network•Starting training with TX-TF1 in

September as a canine handler in state/national disasters

Who is Matt Young•I will treat you as adults, as that is how I

expect you to act (aka, I don’t want to have to call home about poor behavior)

•Anything you tell me in confidence will stay that way.

Who is Matt Young

….and I’m a little dyslexic

If you or your family has a problem with me:

•Chain of Command:▫First talk to me (Mr. Young)▫Then my department head: Charlotte

Wiggins▫Then our department principal: Gwen

Elder

I also need one or two lab managers:

•You would assist in making all media for labs

•General lab prep & lab clean up•You will gain skills in lab equipment

use•Best if you are detail-oriented and/or a

little OCD

My Scholarship

•“AMCHS Scholarship for the Preservation of Natural Resources”

•$1,000•Some of the most important issues facing us

as a species have to do with destruction of habitat, global climate change, and extinction of species.

•My goal is to see more students pursue degrees in Marine Biology, Oceanography, or Natural Resource Management

What you need to Buy/Bring•Bring one or more of the following to help

out with the class (if you can):▫Hand Sanitizer▫Kleenex

Emergency Drills•Shelter in Place•Fire Drill – Taking attendance•Hazardous weather

What are you expecting to get out of the course:

1. Why did you sign up for this course?2. What do you hope to learn?3. From what you’ve learned from prior

science teachers, what I can do to make this the best class possible?

4. What have you enjoyed about prior science courses?

5. What have you hated about prior science courses?

Now About You•Tell me something interesting about

yourself to help me remember you

What we will be covering…1st Semester 2nd Semester

• Introduction to Biotechnology

• The Cell• Lab Procedures• DNA & Genetics• DNA Technologies• Proteins

• Plants• Agricultural

Biotechnology• Advanced Lab

Procedures• Genetic Engineering• Medical

Biotechnology• Animal Biotechnology• Regulations & Ethics

This course is still a work in progress…. All is subject to change!

General Classroom Procedures1. Follow instructions the first time that

they are given.2. Be in your assigned seat when the tardy

bell rings. You must bring notebooks, pen, pencil, & paper.

Tutorial Times:

•Any day before or after school•2nd and 7th period are my “off” periods.

•Email: myoung@csisd.org•(817) 919-9574 (text)•Twitter: @consolbiotech•Consolaquatic.com

Tutorial Times:

Lab Make ups:•You MUST reschedule each lab to make

up with me. •We have several labs each week and they

must be done in order. •Please try not to be absent on lab days.

This will make it harder on you and your lab partner (communication).

•Please email me when you are absent to reschedule any missed labs.

Setting up your Notebook•Create a Table of Contents. This must be

updated daily with ever new page used.▫The main table of contents should need

only one page.

Writing and Effective Experiment Conclusion•3 Parts: REE, PE, PA

▫REE: results with evidence and explanation Answer the purpose question Give data to support your answer Explain support or refute for the hypothesis

▫PE: possible errors Experimental error (do not include things such as

measuring wrong or calculation wrong) Good error: something inherent in the

experiment that may not be as efficient as possible

Give recommendations to improve the experiment, ways to fix the error

Writing and Effective Experiment Conclusion

▫PA: practical application What is an application of the experiment How are the finding valuable What can it do/mean for the future

•Each conclusion must follow the rubric, if you fail to write an effective conclusion you will be required to rewrite the conclusion.

Bellwork: 08/27/2013

1. What is the most common cause of injury in a student laboratory? (very broad answer)

2. What is the first thing you should do if there is an accident in the lab?

3. Where do we meet as a class during a fire drill?

Why use a Lab Notebook…"In the United States, the first person to conceive and show

diligence to develop an invention, product, or process is awarded the patent for that product or process.

Notebooks that are properly prepared, maintained, and witnessed are legal evidence of conception and diligence

to practice an invention”

In short, notebooks can be used for legal proof of ownership. In an industry where billions of dollars are tied to new pharmaceuticals and devices, maintaining proper records is critical. In this class, we will model, to the best

of our abilities, keeping a proper laboratory notebook.

Setting up you Laboratory NB…• Title Page:

Biotechnology Laboratory NotebookYour Name

A&M Consolidated High SchoolPeriod #1/8

Fall 2014 - Spring 2015• The first few pages will be your table of contents• Glue in your LNB Guidelines on the back of the

title page.• Writing Effective Experiment Conclusion will be

glued across from LNB Guidelines• Glue the Unit 1 Lab Rubric at the end of the TOC• You will begin writing up the first lab after the

rubric

Laboratory Guidelines1. Use the notebook for all lab activities. Keep

notes/worksheets in a separate binder.2. Number every page. Date and sign your

name at the end of an experiment.3. Use only blue or black pen. No pencil!4. Do not erase, scribble out mistakes or use

white-out. Cross out mistakes with a single line, like this: the temperature was kept at 37°C

5. Add all sections, procedures, and details required by the teacher.

Laboratory Guidelines6. Avoid abbreviations and codes when

possible. Standard abbreviations such as °C for "degrees Celsius" is acceptable.

7. At times, computer-generated graphs or pictures may be necessary to add. Use glue stick for attaching these items. 

8. Write/print clearly and legibly.9. Skip lines between data tables, graphs, and

important conclusions to make it easier to read.

10.Your notebook will be collected periodically for grading purposes.

Writing labs in your LNB…•Summarize the Background in your own

words. Make sure to include any details•Write down the procedure. Do not write

word for word. You can use a numbered list or a flow chart. ▫you may not use my printed directions

during the lab. You must write the procedures well enough to follow the directions correctly

•Any information in a box needs to be written in a box in your lab notebook and emphasized.

Writing labs in your LNB…•Make sure to leave enough room for data

tables.•All data must be presented in a data table.

Can also be recorded in procedure for ease during lab. (see teacher LNB pg 10)

•All graphs and tables should be glued or taped in your lab notebook. No loose papers

•Many labs will be done back to back. Leave plenty of room for data analysis and the conclusion. If you run out of room add a page extension.

A) What is Biotechnology?•The use of living organisms for human

benefit to make a product or solve a problem

•In the past we have modified whole organisms to solve a problem

•Recently we have started to modify just cells or molecules to solve a problem

A) What is Biotechnology?Ancient Biotechnology

•Focused on food production•Brought plants in from the wild and planted near caves (where they lived)

•Very little food preservation

A) What is Biotechnology?Classical Biotechnology

• Used ancient methods like fermentation to preserve foods

• Breads, cheese, yogurt, alcohols• These products tended to last longer than usual

Bread making in Egypt Making Yogurt

A) What is Biotechnology?Modern Biotechnology

• Began to manipulate genetic material

• Domestication of animals and plants

• Selective breeding or animals and plants to increase the desired traits ▫ From wild mustard we get:

cauliflower, broccoli, cabbage & kale

• Involves manipulating organisms or genes at a microscopic level

Function of Lab Equipment1. Measuring an exact volume of

liquid 2. Used for filtration or the delivery

of liquids between containers3. Tube used to hold microvolumes

(up to ~2 mL)4. Used to heat up substances at a

constant temperature5. Used to heat materials in lab

(uses gas jet at the lab bench)6. Used to find the mass of

materials. 7. Disposable ends placed on

micropipettes8. Commonly used bottle to make

solutions or media9. Tube used to hold small volumes

(up to ~50 mL)10. Used to shake/mix tubes 11. Used to transfer solid chemicals

between containers

12. Used for holding multiple reagents.

13. Used to measure microvolumes of liquid

14. Used to transfer liquids in small volumes (up to ~50 mL)

15. This attaches to a pipette to provide suction

16. Used to hold liquids when a smaller opening is preferred

17. The most common vessel for holding/pouring liquids

18. Used to spin liquids down at high RPMs

19. Used to stir liquids20. Used to heat up/ evaporate

liquids 21. Used to check the temperature

of a substance22. Must be worn for each lab to

protect your eyes

Biotechnology Timeline Activity•Below are the assignments of the years

you and your partner will be researching and presenting

1. Pre 1920s2. 1920-19493. Early & Late 50s4. 1960s & 1970s5. 1980s6. 1990-2000s

B) Common Biotechnology Products• Antibiotics• Leather• Biodegradable plastics

• Biofuel• Vaccines• Pharmaceuticals

Bellwork: 08/29/20131. List and describe the steps in the scientific

method?(I’m sure you’ve heard/learned this a million times so

I’ll only go over it once & briefly)

Scientific Method•Not so much a list of steps as a set of

questioning guidelines1. State a testable scientific question based

on scientific information or observation▫ Usually arises from a previous experiment

2. Develop a testable hypothesis▫ A statement that attempts to answer the

question being asked▫ Also implies how to test and what type of

data should be collected

Scientific Method3. Plan a valid experiment

▫ Usually composed of: Quantitative (numerical) data Multiple replications (several traits) A single manipulated variable (what is

being tested) Control groups:

Positive: one to give predicted result, you know it does what you want to see

Negative: one to give lack of a result, you know it does not do what you want to see

Scientific Method4. Conduct the experiment

▫ Collect and organize data into tables, charts or graphs or graphics

5. Draw a conclusion based on data and error analysis

▫ Also usually suggest any further experiment and applications

▫ Error Analysis is important – every experiment has some kind of error (we do not live in a perfect world)

Scientific Method6. Sharing data in the science world

▫ Usually shared in journals (online and in print)

Safety Lab Info:1. All parts of this lab should be written in

complete sentences.2. Procedure Part One: #8 will not be

found in the laboratory, but you must still name it, explain its use and any safety precautions associated.

3. #20 = Glass Stir rod4. Review Part Two, #15. Part 4: myoung@csisd.org

D) Laboratory Notebooks•Considered to be a legal document•Must be bound and all observations

should be kept in the same journal•Can be used to back up experimental

procedure or patent information•Must be done in pen (pencil can be erased

and changed)•Mistakes should not be blotted out – a line

will allow for mistakes/changes to be seen.

E) Safety equipment•General safety rules apply – see contract•Equipment is expensive – you break it you

buy it▫FYI – the autoclave is over $5000▫Use all equipment with care

•When in doubt ask – better to be safe than sorry

F) Careers in Biotech•Fast growing industry•Education requirements

▫A lab position may require a 2 year technical degree or a 4 year bachelor degree

▫The higher the position and more decision making power, the higher the degree required

•Many positions are non scientific:▫Sales, marketing, human resources,

regulation, legal and administrative staff

F) Careers in BiotechScientific Non-Scientific

• Research and development (R&D)

• Manufacturing and production

• Clinical research• Quality control

• Information systems• Marketing and sales• Regularity affairs• Administration/legal

affairs

Pre Lab: Cheese Production: The Evolution of Cheese Making Technology•Background: Summarize in your own

words•Hypothesis: you do not always have a

hypothesis, but make sure you fill in the blanks with YOUR prediction

•Write the procedure, make sure not to leave out any necessary details.

•When it asked you to record something I suggest you highlight that action and make a place for that information (see teacher LNB pg 10)▫This information still needs to be included

in the data table

Pre Lab: Cheese Production: The Evolution of Cheese Making Technology• If there is a picture or graphic of a setup you

should sketch this in your notebook for reference during the lab.

• Make sure to leave room for data in the data table. ▫I suggest leaving a little more than you think

because often you must correct your numbers after the fact

• You will be printing graphs in data analysis, these MUST be glued or taped into your notebook. They must be attached, they can not float around.▫Be sure to label the axis and give the graph a title.

Time of curdling (in min) for each group.Do not copy each group… this is for

class averaging only!

Agent: 1 2 3 4 5Buttermilk (1)Rennin (2) 5.66 10 10 Avg:

8.55Chymosin (3)

10 10 7 Avg: 9

G) Bioethics•The study of decision making as it applies

to moral decisions that need to be made because of advances in biology, medicine and technology

•Morality is the ability to distinguish between right and wrong▫But not everyone’s morals are the same

Ex: Vegans think it is immoral to eat meat & animal products, not every agrees

•We will discuss various topics through they year

G) Bioethics•Strategies for value clarification:

▫A strategy for examining a bioethical issue to clarify the value one holds

1. Identify and understand the problem or issue, learn as much as possible about the issue

2. List all possible solutions to the issue3. Identify the pros & cons of adopting each

solution Examine the consequences of adopting one

solution over another Consider legal, financial, medical, personal,

social and environmental aspects

Activity: Bioethics: Using Animals in Science and Industry•You will be determining and ultimately

sharing your opinions on using animals in science and in the industry.

•You will work individually with out talking to anyone and follow the directions to fill out the animals in industry chart and complete steps 1-3▫This chart will be glued to the next left

hand page in your INB•We will proceed to step 4 when every one

is ready

Bioethics: Discussion guidelines•As some one is explaining their position do

not interrupt them.

•After each person has had their 2 minutes to present their position, each member will have 1 minute to respond. It is that persons choice to either make a statement or engage in a discussion.

•Remember, every one is entitled to their own opinion, you may not agree but you must be respectful!

Exploring Biotech Careers•Part 1: you will be researching the educational

requirements of a biotechnology program▫You will answer the 3 questions listed

•Part 2: You will research a specific career in the biotechnology field

•All answers and information should be posted on the next available left hand page in your INB.

•Finish for homework if you are not already done.