Week 4- Food and Water Safety f2011student

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    Food and Water Safety

    Definitions

    Food borne Infections

    - Ingestion of ________ microorganisms

    - __________ incubation times

    Food borne Microbial Intoxication

    - Food contains the active toxin

    - Incubation time 24 to 48 hours

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    Water Contaminants

    Physical contaminants

    Presence of particulate matter

    Chemical contaminants

    Industrial activities or agricultural runoff

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    Biological contaminants

    Overabundance of organisms or presence

    of non-native microorganisms

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    CDC Risk- The Top Five

    Shigella dysenteriae

    Escherichia coliO157:H7 (E. coli)

    Salmonella species Vibrio cholerae

    Cryptosporidium parvum (C. parvum)

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    Other,

    - Norwalk virus, Hepatitis A and hepatitis E

    - Helmiths or worms such as tapeworms,

    flukes and round worms

    - Fungi and molds

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    Shigella dysenteriae

    One of four types of __________ bacteria that

    produces a diarrhea inducing, disease causing

    enterotoxin

    All four strains cause dysentery (shigellosis)

    S. dysenteriae, S. flexneri, S. boydii, S. sonnei

    200 cells could cause infection as little affected by

    stomach acids

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    S. Dysenteriae

    Secretes an exotoxin, Shiga toxin, stops protein

    synthesis in its hosts cells

    Pathophysiology

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    E. Coli 0157:H7

    Gram negative rod shaped bacteria with

    _____ virulence (as few as 10 organisms)

    First described as a cause of human illness

    in 1982

    E. coli is the most common type of

    pathogenic infection ___________

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    Sources

    Animal and Other Sources

    - improperly composted manure- contaminated water*

    - spread animal to person and person to

    person, _______________

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    Food Sources

    - undercooked beef- Non pasteurized milk and cheese

    - spinach and other fruits and/or vegetables

    contaminated with feces

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    Pathophysiology ofE.coli0157:H7

    Introduces two types of cells into intestinal

    cells

    Proteins of one type disrupt cell

    metabolism

    Proteins of another type lodge in cell

    cytoplasm and enables this strain to

    displace others

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    Produces _________ inhibits protein

    synthesis, attaches to neutrophil surfaces

    and is spread in body widespread

    death of host cells and tissue

    Antimicrobial drugs __________

    production ofShiga-like toxin

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    S/S

    Some show minimal, or no illness, and

    become carriers

    bloody or watery diarrhea, cramping,

    nausea and vomiting

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    Some may develop hemolytic uremic syndrome

    (HUS)

    Destruction of red blood cells and kidney failure

    3-5% mortality

    http://www.idph.state.il.us/Bioterrorism/factsheets/food_water_b

    http://www.idph.state.il.us/Bioterrorism/factsheets/food_water_borne.htmhttp://www.idph.state.il.us/Bioterrorism/factsheets/food_water_borne.htm
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    Vibrio cholerae

    The only cholerae species that can survive

    in fresh water

    Strain 01 El Tor has caused pandemics

    Strain 0139 Bengal arose in India in 1992

    May be destroyed by stomach acid so size

    of infectious dose 108 cells

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    Pathophysiology

    Cholera toxin enters epithelial cells liningintestine causing loss of sodium andchloride with water following the

    electrolytes causing __________

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    Oral Rehydration Therapy

    Developed by WHO

    Fluid/electrolyte replacement with sodium

    ______________________________________

    dissolved in water (similar to Pedialyte)

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    Clostridium Botulism

    Spore forming anaerobe in water and soil

    and, occasionally, animal intestinal tract

    Human and animal distinctly different

    types

    Botulin is the most potent microbial toxin

    known to mankind

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    Food Sources of Botulism

    Spores are present on vegetables or meat at

    time of gathering

    Reasons for Spread

    ________ cooking does not reach

    sufficient pressure or temperature to kill

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    Processing removes oxygen encouraging

    growth

    Room temperature storage favors spore

    germination and growth

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    Pathophysiology and S/S

    Botulin prevents the uptake of theneurotransmitter substance acetylcholineat the neuromuscular junction

    Muscles of head- double vision, difficulty inswallowing but no cognitive lapse

    Causes descending muscular paralysis andrespiratory compromise with death fromrespiratory failure

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    Norwalk Virus (Norwalk, Ohio)

    Cup-shaped enteric virus transferred

    orofecally from human to human

    Reservoir is _______

    camps, day care, cruise ship, nursing home

    Gastroenteritis- acute onset with N/V,

    cramps, diarrhea, and chills recovery

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    Hepatitis

    Viral infections of liver necrosis of

    hepatocytes and liver inflammation

    S/S may include jaundice, malaise, fever,

    chills, diarrhea, abdominal pain, and

    blood abnormalities

    Outcome is recovery to risk of,..

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    Food Safety Handling Tipshttp://www.dukehealth.org/health_library/care_guides/cancer/tre

    Buy refrigerated foods that are cold and frozen

    food that is solid. Shop for these last and get

    them home quickly ___________________________________

    Place raw meat and poultry in separate plastic

    bags to prevent dripping on other foods

    Wash your hands with soap and running water

    before and after preparing foods

    http://www.dukehealth.org/health_library/care_guides/cancer/treatment_instructions/preventing_food_and_water_borne_illnesshttp://www.dukehealth.org/health_library/care_guides/cancer/treatment_instructions/preventing_food_and_water_borne_illness
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    Cook meat and poultry to > 165F and eggs untilfirm

    Put cooked meat and poultry on a clean platter

    Separate raw and cooked foods to avoid crosscontamination

    Be careful not to use the same cutting board formeats and vegetables

    Wash all surfaces and supplies thoroughly Refrigerate leftovers promptly with maximum of

    two hours

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    Reheat leftovers to > ________

    Bring soups, sauces and gravies to a rolling boil

    and then simmer for ten minutes

    Avoid preparing food for others if you are ill

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    Prevention of Food Spoilage

    [Thompson, Manore, Sheeshka)

    Salting and sugaring

    Drying

    Smoking

    Cooling

    Industrial canning

    Pasteurization

    Addition of preservatives

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    Water Contaminants

    Previously done,

    Vibro cholerae Shigella spp.

    Salmonella spp. E. coli Norwalk Clostridium Botulism

    Giardia intestinalis (lamblia)

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    Giardia intestinalis

    Pathogenic flagellate with________

    Infection from flagellate ______ which cansurvive for __________ months in the

    environment

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    Reservoir

    intestinal tracts of humans and animals

    (beaver, coyotoes, cats,) world-wide

    Water- mountain stream clean and

    municipal as chlorine amounts insufficient

    [ozone, iodine, boiling]

    soil, feces-contaminated surfaces

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    Pathophysiology and S/S

    Cysts enter duodenum, __________ and

    travel to jejunum damage to epithelium

    [reversible]

    Diarrhea, abdominal pain and flatulence

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    Steps in Water Treatment

    [Burton text]

    Filtration- removal of large debris ie. Branches

    Sedimentation- water rests in holding tank

    allowing debris to settle to the bottom

    Coagulation- Aluminum potassium sulfate added

    to resting water to clump the debris

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    Filtration- water passes through sand, earth filters,charcoal filters or membrane filters to removeremaining bacteria, protozoas, and others suchas C. Parvum

    Chlorination- 0.2 to 1 ppm chlorine gas or othersubstance. In some facilities ozone or ultraviolet

    light may be used instead

    Multiple places for errors so need to check water