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MEDT 251 Goals Schedule Faculty Policies NiceNet class Objectives: cognitive, psychomotor, affective Offices and faculty Resources
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Week 1: Orientation
• Orientation• Biological safety• Chemical safety• Fire safety
• Lab glassware• Pipetting
MEDT 251
• Goals• Schedule• Faculty• Policies• NiceNet class• Objectives: cognitive, psychomotor, affective• Offices and faculty• Resources
Pipets
• Transfer fluids• Make dilutions• Make primary and secondary standards• Reconstitute reagents and controls
Why so many kinds?
Pipet Variation…
• Types of fluid• Blood, plasma, serum• Aqueous reagents• Viscosity
• Degree of accuracy needed• Accuracy vs. efficiency
• Amount of fluid
Markings on Pipet
• Total volume and increments• Calibration temperature: 20oC• TD: to deliver• TC: to contain• Tolerance limits
Etched band means “blow out”
Color bands just indicate size
General Types of Pipet
• Volumetric: for accuracy• Volumetric transfer• Ostwald
• Graduated: for efficiency• Serological• Mohr
Pipetting1. Select the right pipet2. Check for broken tip3. Aspirate with bulb4. Wipe the tip5. Adjust meniscus6. Deliver along side of vessel7. Place in wash receptacle
Checking…
No!
Tip is completely in the fluid
Wipe the tip after aspiration
Dispense fluid along side of vessel
Division Safety Rules
• Read the Division’s safety manual
• Sign the Assumption of Risk and Release form