SummaryThe Summary section should have a short statement of the background, justification for analysis by light scattering or other tech-niques supported by Wyatt, and the key takeaways of the app note or white paper. Two-three paragraphs is usually enough. The primary goal of the summary is to en-tice the readers to read the rest, or to un-derstand that it does not really hold any-thing of interest and to move on to the next app note.In some instances you might want to in-clude a copy of a key data graph in the summary. It can have a caption, but if a du-plicate appears later, do not number it.

IntroductionIntroduce the subject of the application note, the importance to the world, and the challenges such as where other characteri-zation techniques fail or the importance of high-throughput, etc. Break the text up into paragraphs gener-ously, it makes it easier to read. Don’t skimp on graphics, graphs of data and ta-bles of results. Most Figures should fit into a column but as shown below, you can also have them spread across the page in a sin-gle-column section between two-column sections

Subsection 1The first instance of each product such as DAWN®, miniDAWN® or microDAWN, should have the registered trademark or

as appropriate, and be linked to the web site using the respective URL mnemonic shortcut. The list of shortcuts is in the Sup-port Center under https://www.wyatt.com/files/literature/sales/Wyatt-web-shortcuts.-docx. Techniques such as SEC-MALS, DLS, etc should also be referenced to the web site, and where appropriate other pages that have shortcuts such as Protein Applica-tions, webinars, the Bibliography or theory pages.

In the introduction you might include one or two figures that do not have numbered cap-tions. Some examples are a decorative graphic, an image of the instrument, a chemical representation or a ribbon repre-sentation of the protein being studied. Link references to figures and tables appro-priately. An example is presented in .

Graph specs: All graphs should be 6.8 cm high by 7.5

cm wide. Axis titles should be Calibri 11 Axis labels should be Calibri 10 with ticks

outside the graph.

WPXXXX: SEC-MALS Determination of a Property of a SampleAuthor, Affiliation†

Callouts and legends should be Calibri 8 – 10.

There should be a thin (0.5 – 1 point) line around the plot region, including the axes.

Plots should be distinguished by color and line type or symbol type.

For convenience, graphs are produced in Powerpoint at twice the size, then copied and pasted as images into the Word doc and the width set to 7.5 cm.

The image should be centered rela-tive to the column.

Set top and bottom margins of 12 points.

Figure 1. Do not use screen shots from the software or copied graphs unless you can make the labels, axis titles and legend items clearly legible and uniform across the application note. It is better to export to Excel and create uniform size and styling.

Another way to achieve quality graphs is to copy and paste into Powerpoint, size appro-priately, retype the axis labels, titles, and legends in text boxes, add any callouts or arrows, and crop the graph to remove the sections that were retyped. Then copy the whole thing and paste as a picture into the Word doc.

Materials and MethodsThis section might not be relevant to a white paper. If it is, describe in brief the an-

alyte, instrument setup and procedures. This will help the reader understand what it’s about and how easy it is to do. Don’t for-get to mention the Wyatt software used to collect and analyze data, with correct capi-talization, trademark, and link: ASTRA®, DYNAMICS®, CALYPSO®, SCOUT DPS®, etc. Sometimes the layout looks best if you move a new section to the top of the next column by inserting a Column break, from Layout->Breaks->Column.

Results and DiscussionThis section might not be relevant to a white paper. If it is, include key results, with graphs and tables, and a description of the information present in each. Remember that the reader may not be familiar with the sub-ject matter or instrumentation, but we still want the application note to appear mean-ingful. Can you make these results relate to other areas of research and characterization by wording the text in a broadly applicable manner? Can you cater to both the experts – with specific results – and those who may not be from this field with more general statements?Table 1. Some results are best presented this way.

Laser (nm)

Interference Filters (20 nm)

Sample 1App. MW (kDa)

Sample 2App. MW (kDa)

665 No 89 ± 6 176 ± 6

665 Yes 30 ± 5 34.4 ± 5

786 No 30.7 ± 0.3 24.4 ± 0.1

786 Yes 27.2 ± 0.3 14.2 ± 0.1

Don’t just say ‘here, we made a measure-ment’. What have you learned from the analysis? How did it benefit science and hu-manity? What did our instruments contrib-ute that would not otherwise have been possible, whether in quality, quantity, time, effort, etc? What other Wyatt techniques could be applied to flesh out the under-standing?One angle: Ut id arcu ullamcorper, efficitur neque nec, tempor massa. Fusce lacus mau-ris.Another angle: Sed scelerisque quam non mi finibus rutrum sit amet in justo. Proin eleifend est non venenatis rutrum.

ConclusionsMention both the overall relevance of the analysis to science, industry, humanity, etc. and the key takeaways regarding the bene-

fits of light scattering and other Wyatt in-struments/techniques.

AcknowledgementsWyatt author: Thank a customer for providing samples, additional data, etc. The conjugated nanoparticle samples were kindly provided by Dr. John Doe at the Home Town University De-partment of Frogs.Customer author: funding sources or whomever you need to acknowledge.If no acknowledgements then delete this sec-tion.

References If no references, delete this section. If possible, use Mendeley Reference Management software and select Nature format.

1. Mika, M., N. Marianne Utilization of Mem-brane Processes in Treating Various Efflu-ents Generated in Pulp and Paper Industry, in Handbook of Membrane Separations, 981-1006 (CRC Press, 2008).

2. Albinsson, B. et al. The origin of lignin fluo-rescence. J. Mol. Struct. 508(1–3), 19- 27 (1999).

3. Fredheim, G.E., Braaten S.M., Christensen, B.E. Molecular weight determination of lig-nosulfonates by size-exclusion chromatog-raphy and multi-angle laser light scatter-ing. J. Chromatography A 942(1–2), 191-199 (2002).

4. 4. Ringena, O. et al. Size-exclusion chro-matography of technical lignins in dimethyl sulfoxide/water and dimethylacetamide. J. Chromatography A 1102(1–2), 154-163 (2006).

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This is a closing graphic, optional (no caption). You can find various such images in the Marketing folder under Graphics\Instru-ment Illustrations or Graphics\Instrument Images.

The table below is set to float over the page. So if you run out of room for it, please reformat in order to get a new page. Be sure to edit the list of trademarked products per the content of the application note.