Waters HPLC Competitive Review

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Varian 920-LC Market Competitors

Competitors• Shimadzu• Waters• Agilent• Dionex

•The 920-LC presents a significant step forward for the Varian

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Waters Alliance

• Consists of two versions • 2695 Separations Module• 2795 Separations Module

• Pump, autosampler, column heater and controller

• Wide range of Detectors– 2996 Diode Array Detector– 2487 Dual wavelength Detector– 2475 multi-wavelength Detector– 2414 RI Detector– 432 Conductivity Detector– 2420 ELS Detector– 2465 Electrochemical Detector

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Varian 920-LC Compared to Waters Alliance

Waters Alliance Varian 920-LC

Maximum flowrate 5 ml/min 10 ml/min

Maximum pressure

Autosampler Capacity

120 HPLC vials 200 HPLC vials

Microplate Capability NA 3 plates

Maximum Column Heater Temp

60oC 65oC

Number of Detectors None Up to 2 built-in

Sample Carryover <0.1% <0.01%

Varian 940-LC Compared to the Waters Purification HPLC

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Waters Delta 600 LC Analytical to Prep

• Delta 600 wide flow rate range and 2 way injector valve allows operation at analytical and prep scales

• Delta 600 LP

– LP dual piston quaternary gradient pump• 0.01 to 20 ml/min & up to 6000 psi• Up to 45 ml/min option

• Stand alone 2 channel degasser option recommended

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PrepLC Dedicated Purification Systems

• PrepLC 150 and 300 – Dedicated preparative LC system quaternary gradient– 1 to 150 ml/min

• Up to 4000 psi

– 4 to 300 ml/min• Up to 2000 psi

• DeltaPrep 150 and 300– Same as PrepLC but with solvent recycling capabilities

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Waters PrepLC

Sample Load Column ID Model Max Flow Rate

(ml/min)

mg – 10’s g 7.8 – 75 mm DeltaPrep 300 300 ml/min

g – g 4.6 – 50 mm DeltaPrep 150 150 ml/min

g – 10’s mg 4.6 – 50 mm 2545 150 ml/min

g – 10’s mg 3.9 – 30 mm Delta 600 EF 45 ml/min

g – 10’s mg 3.9 – 19 mm 1525 + EF 22.5 ml/min

g – 10’s mg 3.9 – 19 mm Delta 600 20 ml/min

g - g 3.9 – 7.8 mm 1525 10 ml/min

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Summary of Varian 940-LC vs Waters Purification Systems

• Limited automation

– No autosampler injection capability

• Limited fraction collection capability

– Front panel control with limited S/W interaction

– Up to 120 test tubes or 2 Alliance Carousel racks (48 x 2 ml HPLC vials)

• Inflexible pumping system– Need two systems to cover 4.6 mm, 21 mm and 50 mm ID

columns

• Analytical detectors with short path flow cells

– Limited performance in analytical mode– Or limited abs range

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Waters Detectors for Prep LC

Questions??