Viral RNA / DNA purification products from MACHEREY-NAGEL ... · MACHEREY-NAGEL GmH Co G Neumann-Neander-Str 6-8 52355 Dren ermany Sierlan MACHEREY-NAGEL AG Tel 41 0 62 388 55 00

MACHEREY-NAGEL GmbH & Co. KG · Neumann-Neander-Str. 6-8 · 52355 Düren · GermanySwitzerland:MACHEREY-NAGEL AGTel. : +41 (0) 62 388 55 00Fax : +41 (0) 62 388 55 05E-mail : [email protected]

Germanyand international:Tel. : +49 (0) 24 21 96 90Fax : +49 (0) 24 21 96 91 99E-mail : [email protected]

France:MACHEREY-NAGEL EURLTel. : +33 (0) 3 88 68 22 68Fax : +33 (0) 3 88 51 76 88E-mail : [email protected]

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USA:MACHEREY-NAGEL Inc.Tel.: +1 484 821 0984Fax: +1 484 821 1272E-mail: [email protected]

Nuc

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sViral RNA / DNA purification productsfrom MACHEREY-NAGEL

Viral RNA and DNA for In-Vitro DiagnosticsCE-IVD marked isolation of viral RNA and DNA from human plasma and serumNucleoSpin® Dx Virus

CE-IVD marked in accordance with EU Directive 98/79/ECFor EDTA and citrate treated samplesHighly sensitive and reliable extraction... even from a broad range of viral titers

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NucleoSpin® Dx VirusViral RNA and DNA from human plasma and serum for in-vitro diagnosticsXX CE-IVD marked in accordance with EU Directive 98/79/EC

Compliant with IVD directives in the EU

XX Fits into in-vitro diagnostic workflows CE-marked viral RNA and DNA isolation from plasma and serum CanbecombinedwithcommonenzymaticamplificationanddetectionofviralRNAorDNA

XX Compatible with fresh or frozen samples treated with EDTA or citrateXX Highly consistent viral RNA and DNA isolation

Reproducible isolations from different titers for reliable downstream applications

XX Suitable for animal samples The intended use allows veterinary applications, which are non-IVD

Procedure

Product at a glanceTechnology Silica-membrane technologyFormat Mini spin columnsSample material Plasma or serum, fresh or frozen, EDTA or citrate treatedSample volume 150μLElution volume 50μLProcessing CentrifugationPreparation time ~30 min / 4 – 6 preps

plasmaserum

MixsampleandLysisBuffercontainingcarrierRNAAdd Proteinase K for viral DNA extraction5 min incubation

Add ethanol and transfer lysate ontoNucleoSpin® Dx Virus Column

Sample lysis

Binding of viral RNA / DNA

Washing

Elution of viral RNA / DNA

Pipette 1stWashBuffer(highsaltconcentration)Pipette 2ndWashBuffer(lowsaltconcentration)

Elutein50μLRNase-freewaterorelutionbuffer

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Reliable isolation of viral nucleic acids for diagnostic workflows

NucleoSpin® Dx Virus is a generic kit which can easily be integrated into in-vitrodiagnosticworkflows.Thekitallowsthepurificationofnucleicacidfromdifferentvirusesandcanbeusedincombinationwithabroadrangeofdownstreamassaysystems.ThesystemissuitableforfreshandfrozensamplestreatedwithEDTAorcitrate.

Application dataCE-marked procedure – sensitive viral DNA and RNA detection

DetectionofviralRNAandDNAinReal-timePCR(ArtusRealArtHCVRNA/HBVDNA;RocheLightCycler®480),purifiedbyNucleoSpin®DxVirus.Nucleicacidswerepurifiedfromvirussamplesofdifferenttitersandusedforquantification.A)DetectionofRNAfromHCVsamplesofdifferenttiters.B)DetectionofDNAfromHBVsamplesofdifferenttiters.DatawasgeneratedbyDr.Tiemann,LABCON-OWLGmbH,Germany.

High reproducibility of isolation and detection

ReproducibilityofHCVandHBVdetection.RNAandDNAfromvirussamplesofdifferenttiterswereisolatedintriplicatebyNucleoSpin® Dx Virus fordownstreamdiagnosticquantification.A)DetectionofRNAfromHCVsamplesofdifferenttiter.Sample1:negativecontrol,sample2:positivecontrolatlowestdetectionlimit,sample3–9:differentHCVtiter.Detection:ArtusRealArtHCVRNA;RocheLightCycler®480.B)DetectionofDNAfromHBVsamplesofdifferenttiter.Sample1:negativecontrol,sample2:positivecontrolatlowestdetectionlimit,sample3–11:differentHBVtiter.Detection:ArtusRealArtHBVDNA;RocheLightCycler®480.DatawasgeneratedbyDr.Tiemann,LABCON-OWLGmbH,Germany.

Sample collection(serum or plasma)

Nucleic acid isolation(NucleoSpin® Dx Virus)

Downstream detection system

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Ordering information

Single prep (spin columns) – CE-IVD marked Preps REF

NucleoSpin® Dx Virus 50 / 250 740895.50/.250CE-IVDmarkedMinispinkitfortheisolationofviralDNAandRNAfrom150μLhumanplasmaorserum.

Related productsSingle prep (spin columns) – CE-IVD marked

NucleoSpin® Dx Blood 50 / 250 740899.50/.250CE-IVDmarkedMinispinkitfortheisolationofgenomicDNAfrom200μLhumanwholeblood.Single prep (spin columns) – not CE-IVD marked

NucleoSpin® RNA Virus 10 / 50 / 250 740956.10/.50/.250MinispinkitfortherapidisolationofviralRNAfromplasmaorserum.ForviralDNAisolation,ProteinaseKisrequired(740506).NucleoSpin® RNA Virus F 25 740958SpinkitforRNAisolationfromcell-freebiologicalfluidswithfunnelshapedcolumns.Largesamplesofupto1mLcanbeprocessedandelutedinsmallvolumes(50–100μL).NucleoSpin® Plasma XS 10 / 50 / 250 740900.10/.50/.250MinispinkitfortherapidpurificationofcirculatingDNAfromplasmaorserum.Highrecovery,evenofshortDNAfragments(downto50bp).ConcentratedDNAeluateduetoXScolumndesign(elutionin5–30μL).Medium and high throughput – not CE-IVD marked

NucleoSpin® 8 Virus 12 x 8 / 60 x 8 740643/.58-wellstripsformediumthroughputisolationofviralRNAandDNA.NucleoSpin® 8 Virus Core Kit 48 x 8 740451.4Corekitwithbasiccomponents:NucleoSpin®VirusBindingStrips,buffers,CarrierRNA.NucleoSpin® 96 Virus 2x96/4x96 740691.2/.496-wellplatesforhigh-throughputisolationofviralRNAandDNA.NucleoSpin® 96 Virus Core Kit 4x96 740452.4Corekitwithbasiccomponents:NucleoSpin®VirusBindingPlate,buffers,CarrierRNA.NucleoMag® 96 Virus 1x96/4x96 744800.1/.4IsolationofviralRNAandDNAwithmagneticbeads.

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MACHEREY-NAGEL GmbH & Co. KG · Neumann-Neander-Str. 6–8 · 52355 Düren · GermanySwitzerland:MACHEREY-NAGEL AGTel.: +41 62 388 55 00Fax: +41 62 388 55 05E-mail: [email protected]

Germanyand international:Tel.: +49 24 21 969-0Fax: +49 24 21 969-199E-mail: [email protected]

France:MACHEREY-NAGEL EURLTel.: +33 388 68 22 68Fax: +33 388 51 76 88E-mail: [email protected]

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Nuc

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Genomic DNA Mini spin kit for in-vitro diagnosticsCE-marked for gDNA isolation from whole bloodNucleoSpin® Dx Blood

CE-marked in accordance with EU Directive 98/79/ECFor EDTA, citrate, and heparin blood samplesVery convenient handlingHighly reproducible gDNA isolation from whole blood…… for most reliable results in PCR!

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NucleoSpin® Dx BloodgDNA from whole blood – for in-vitro diagnostic purposesXX CE-marked in accordance with EU Directive 98/79/EC

Compliance with IVD directives in the EU

XX Fits into in-vitro diagnostic workflows CE-marked gDNA extraction from whole blood CanbecombinedwithanyenzymaticamplificationanddetectionofgDNA

XX Compatible with common blood collection tubes and anticoagulants Suitable for EDTA, citrate, and heparin blood collecting systems For fresh and frozen blood samples

XX Highly reliable DNA isolation from whole blood Reproducible results for reliable downstream applications

XX Convenient handling Product storage: at room temperature Precise photometric measurement of DNA possible

ProcedureNucleoSpin® Dx Blood is based on well-established NucleoSpin® silica-membrane technology and provides an easy waytoisolategenomicDNAfrom200μLofwholeblood.ThebloodsamplesarelysedinpresenceofchaotropicsaltsandProteinaseK.Subsequently,genomicDNAisboundto the NucleoSpin®DxBloodColumn.TheDNAonthemembraneiswashedandfinallyhighlypuregenomicDNAiseluted.

Mix Proteinase K, blood sample, and lysis buffer15 min incubation

Transfer lysate onto NucleoSpin® Dx Blood Binding Column

Pipette 1st wash buffer(high salt concentration)Pipette 2nd wash buffer(low salt concentration)

Elute DNA in 50–200 μL elution buffer

1. Sample lysis Efficient lysis of fresh or frozen blood (EDTA, citrate, or heparin treated)

2. Binding of DNA Lysate is loaded in one step

3. Washing Removal of inhibitors and contaminants by two washing steps

4. Elution of genomic DNA Elution in azide free elution buffer, allowing direct photometric DNA quantification

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NucleoSpin® Dx Blood Product at-a-glanceTechnology Silica-membrane technologyFormat Mini spin columnsSample material Whole blood, fresh or frozen

EDTA, citrate, or heparin treatedSample volume 200μLTypical DNA yield 3–5μg(dependingonindividualbloodsample)TypicalDNAquality Ratio A260/A2801.7–1.9Elution volume 50–200μLTypical DNA concentration 40–60ng/μLProcessing CentrifugationPreparation time 30 min

Application dataCE-marked procedure – excellent DNA quality – reliable downstream applications

Excellent DNA recoveryDNAwasisolatedfromtriplicatesofbloodsamples(200μL,EDTA)from5individuals(A–E).TheDNAyieldis2.7–6.6μg,dependingonbloodsample.

Consistent high purityRatio A260 /A280 was measured for 15 DNA samples(triplicates,from5individuals,A–E).Theratioisconsistentlybetween1.80and1.92indicatingexcellentDNAquality.ConsistencyinDNAqualityforbestperformanceinIVDworkflows.

Reliable performance regardless the anticoagulant usedDNA was isolated from 15 individual blood samples, stabilizedwithdifferentanticoagulants:EDTA,citrate,andheparin.Allsamplesareshowingreliablegoodperformanceinq-PCR.

LightCycler®(Roche)q-PCR,β-globinspecificprimer

Compatible with common blood sampling devices, e.g.,Blood collecting systems Manufacturer

S-Monovette®Li-Heparin SarstedtS-Monovette® EDTA SarstedtS-Monovette® Citrat SarstedtVACUETTE® EDTA GREINER BIO-ONEBD VACUTAINER® K2E BD DiagnosticsK2 EDTA APTACA

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MACHEREY-NAGEL GmbH & Co. KG · Neumann-Neander-Str. 6–8 · 52355 Düren · GermanySwitzerland:MACHEREY-NAGEL AGTel.: +41 62 388 55 00Fax: +41 62 388 55 05E-mail: [email protected]

Germanyand international:Tel.: +49 24 21 969-0Fax: +49 24 21 969-199E-mail: [email protected]

France:MACHEREY-NAGEL EURLTel.: +33 388 68 22 68Fax: +33 388 51 76 88E-mail: [email protected]

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Your local distributor

NucleoSpin® Dx BloodReliable DNA isolation for IVD workflowsNucleoSpin®DxBloodisagenericsystemfortheisolationandpurificationofgenomicDNAfromhumanwholebloodsamples forsubsequent in-vitrodiagnosticpurposes.Thekitcanbeusedwith freshand frozenhumanwholeblood treatedwithEDTA,citrate,andheparin,fromcommonbloodcollectionsystems.NucleoSpin®DxBloodisdesignedtobeusedwithanydownstreamapplicationemployingenzymaticamplificationanddetectionofDNA(e.g.,PCR)andthusfitsperfectlyintodiagnosticworkflows.

Ordering informationSingle prep (spin columns) – CE-IVD marked Preps REF

NucleoSpin® Dx Blood* 50 / 250 740899.50/.250CE-IVDmarkedMinispinkitfortheisolationofgenomicDNAfrom200μLhumanwholeblood.

Related productsSingle prep (spin columns) – CE-IVD marked Preps REF

NucleoSpin® Dx Virus 50 / 250 740895.50/.250CE-IVDmarkedMinispinkitfortheisolationofviralDNAandRNAfrom150μLhumanplasmaorserum.

Single prep (spin columns) – not CE-IVD marked Preps REF

NucleoSpin® Plasma XS 10 / 50 / 250 740900.10/.50/.250Minispinkit(XSdesigncolumns)fortherapidpurificationofcirculatingDNAfromplasmaorserum.

NucleoSpin® Blood 10 / 50 / 250 740951.10/.50/.250MinispinkitfortheisolationofDNAfromupto200μLblood.

NucleoSpin® Blood L 20 740954.20MidispinkitfortheisolationofDNAfromupto2mLblood.

NucleoSpin® Blood XL 10 / 50 740950.10/.50MaxispinkitfortheisolationofDNAfromupto10mLblood.

Medium and high throughput – not CE-IVD marked Preps REF

NucleoSpin® 8 Blood 12 x 8 / 60 x 8 740664/.5NucleoSpin® 8 Blood Core** Kit 48 x 8 740455.4NucleoSpin® 96 Blood 1x96/4x96/24x96 740665.1/.4/.24NucleoSpin® 96 Blood Core** Kit 4x96 740456.48-wellstrips/96-wellplatesforautomatedormanualisolationofgenomicDNAfromwholeblood.

NucleoMag® Blood 200 μL 1x96/4x96 744501.1/.4NucleoMag® Blood 3 mL 1x96 744502.1Formanualorautomated,magnetic-beadbasedisolationofgenomicDNAfromwholeblood.

Visit www.mn-net.com/bioanalysis for detailed information

*IVD-CE-marked kit: Not available in all countries, please inquire.** Kits with basic content focussed on automation platforms.

Additional accessories can be combined as needed.

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Blood collection system (EDTA, citrate, heparin)

NucleoSpin® Dx Blood (CE-marked) PCR amplification

Nu

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Genomic DNA Mini spin kitUnlimited use with maximum performance!NucleoSpin® Tissue

ForensicsVeterinary testingGenotypingBiological and medical research

Count on validated quality

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NucleoSpin® Tissue Choose NucleoSpin® Tissue for your genomic DNA isolation and take advantage of our experience in DNA extraction!

Enhance your flexibility DNA isolation from a wide variety of sample materials, covering clinical and forensic samples, tissues, cells, yeast, bacteria, blood, buffy coat, and viruses. More than 16 support protocols are available, optimized for your demands.

Increase DNA yield and performance Highly sensitive silica membrane technology giving you maximum yield and purity. PCR inhibitors are removed effectively.

Get reliable results High quality DNA, validated in numerous downstream applications including genetic fingerprin- ting, real-time PCR, restriction enzyme digests, and sequencing.

Product at-a-glance

Technology: Silica-membrane technology Format: Mini spin columns Sample material: 1 – 25 mg tissue; 102 – 107 cells Fragment size: 200 bp to >30 kbp Typical yield: 20 – 35 μg Binding capacity: 60 μg Typical Ratio A260/A280: 1.7 – 1.9 Elution volume: 60 – 100 μl Preparation time: ~ 20 min/prep (excl. lysis)

y DNtime PCR,

References

NucleoSpin® Tissue shows proven reliability in DNA purification from different sample materials.Following table presents a selection of peer-reviewed publications citing NucleoSpin® Tissue.

Sample material DNA application Publication

Dried blood spots on DNA virus detection by PCR C. S. Gibson et al., BMJ 332, 2006newborn screening cards

Buccal swabs PCR of Y-chromosomal STR loci H. Rodig et al., Int J Legal Med. 121(1), 2007

Mice ear markings PCR of Car9 and Car2 gene targets P. Pan et al., J. Physiol. 571, 2006

Cells (ciliate Oxytricha trifallax) PCR M. Nowacki et al., Nature 451, 2007

FFPE tissue (Formaline Fixed Determination of the methylation R. Schneider-Stock et al., J. Clin. Oncol. 21, Paraffin-Embedded) status of a promoter 2003

Ants (ethanol preserved) PCR, target: mitochondrial DNA, R. Savolainen and K. Vepsäläinen, PNAS 100, 2003 cytochrome oxidase

Rat tails and Genotyping to distinguish between B. Pardo et al., J. Biol. Chem. 281(2), 2006embryonic tissue wild type and aralar deficiant animals

Microdissected frozen and/or Mutation analysis using PCR and V. Máximo et al., British Journal of Cancer 92, paraffin-embedded tissue automated sequencing 2005

Malignent melanoma Array-CGH and mutation analysis G. Jönsson et al., Oncogene 26, 2007

Wasp leg, small pieces PCR, a 658-bp target, near the 5 M. A. Smith et al., PNAS 105(35), 2008(1 mm long) terminus of the CO1 gene

Cells, parental and lentivirally PCR, Target: HSV-TkEGFP R. Uch et al., Cancer Gene Therapy 10, 2003 transduced

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Fig. 2: Agarose gel electrophoresis of genomic DNA.

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Application Data

Clinical Application

Detection of CMV virus in different clinical samplesCytomegalovirus (CMV) belongs to the family of Herpesviridae that contains large double stranded DNA genomes. The virus is widely spread and transferred by direct contact. The detection of CMV from different matrices requires sensitive purification of genomic DNA as well as effective removal of PCR inhibitors for a successful subsequent DNA amplification.

Figure 1 shows PCR results of DNA purified from urine, liquor, feces, mother's milk, and plasma with NucleoSpin® Tissue. CMV can be detected in mother's milk as well as in several samples from the child (marked with arrows). The other samples do not carry the virus.

NucleoSpin® Tissue allows successful DNA isolation from a largevariety of clinical samples.

1: marker 2: urine sample from child 3: liquor sample from child 4: feces sample from child 5: plasma sample from mother 6: mother's milk 7: urine sample from mother 8: positive control 9: negative control 10: 100 bp ladder

Data kindly provided by Dr. Tiemann, Laboratory Prof. Hagedorn, Herford, Germany

Zoological Research

Isolation of DNA from aged tissue samples (wood mouse, Apodemus sylvaticus)Ethanol preserved samples of wood mouse tissue (25 mg) were subjected to DNA isolation with NucleoSpin® Tissue following the standard protocol. The ages of the samples were 1 year (sample 1), 44 years (sample 2), and 102 years (sample 3).

Figure 2 shows gel electrophoresis of the isolated genomic DNA which was successfully isolated from all three specimens. High molecular DNA was isolated from sample 1. DNA fragments of samples 2 and 3 are shorter due to the age of the samples. Yield and purity of DNA were measured with NanoDrop™.

NucleoSpin® Tissue allows the isolation of highly pured DNA, even from aged samples.

Sample 1 2 3

Age 1 year 44 years 102 years

Yield 9.9 μg 6 μg 2.9 μg

Ratio A260/A280 1.79 1.82 1.89

L: DNA Ladder (GeneRuler™ 100 bp Plus; 100-3000 bp) 1: Genomic DNA from 1 year old sample 2: Genomic DNA from 44 year old sample 3: Genomic DNA from 102 year old sample

Data kindly provided by C. Etzbauer and C. Blume; Zoologisches Forschungsmuseum (zoological research museum) Alexander König, Bonn, Germany

Fig. 1: Agarose gel electrophoresis of a CMV specific nested PCR product (495 bp)

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MACHEREY-NAGEL GmbH & Co. KG · Neumann-Neander-Str. 6-8 · 52355 Düren · GermanyGermany USA France SwitzerlandTel.: +49 (0) 2421 969 270 Tel.: +1 610 559 9848 Tel.: +33 (0) 388 682268 Tel.: +41 (0) 62 388 55 00e mail: tech bio@mn net.com e mail: sales us@mn net.com e mail: sales fr@mn net.com e mail: sales ch@mn net.com

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EN ISO 9001 2000CERTIFIED

Ordering informationProduct Preps Cat. No.

Mini spin columnsNucleoSpin® Tissue 10/50/250 740952.10/.50/.250Mini spin kit for the isolation of genomic DNA from a wide variety of samples. Optimized protocols - validated for numerous applications.

Related productsXS spin columnsNucleoSpin® Tissue XS 10/50/250 740901.10/.50/.250Mini spin kit for the isolation of highly concentrated genomic DNA from Xtra Small samples. Allows elution in only 5 μl.

Funnel columnsNucleoSpin® DNA Trace 4/25 740942.4/.25 Funnel columns for forensic samples. Allows sample extraction in high volume of lysis buffer for highest flexibility.

Medium and high throughput solutionsNucleoSpin® 8 Tissue 12x8/60x8 740740/.5 8-well strips for your flexibility. For manual or automated high throughput isolation of genomic DNA.

NucleoSpin® 96 Tissue 2x96/4x96/24x96 740741.2/.4/.2496-well plates for fast parallel preperations of 96 samples. For manual or automated high throughput isolation of genomic DNA.

Magnetic Bead Technology – Medium and high throughput solutionsNucleoMag 96 Tissue 1x96/4x96/24x96 744300.1/.4/.24For manual or automated isolation of genomic DNA.

Visit www.mn-net.com/bioanalysis for detailed information

Your local distributor:

Application Data (cont.)

Veterinary Testing

Isolation of genomic DNA from koi (Cyprius carpio) and amplification in real-time TaqMan® PCRNucleoSpin® Tissue was used to isolate DNA from samples of three Koi gills. Koi glucokinase was amplified in real-time TaqMan® PCR, followingthe protocol described by Gilad et al. (2004). Figure 3 shows the results of the successful amplification of all tested samples.

NucleoSpin® Tissue for reliable isolation of high quality genomic DNA from veterinary samples.

Red: Koi gill 1 Blue: Negative controlGrey: Koi gill 2 Yellow: No template controlGreen: Koi gill 3

Data kindly provided by the Staatliches Veterinäruntersuchungsamt(Governmental Veterinary Testing Department) Arnsberg, Germany

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Plan

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Prod

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from MACHEREY-NAGEL

DNA or RNA from plant samplesIsolations don‘t rely on luck...NucleoSpin®

NucleoMag®

Highest lysis efficiencySuperior yield and purityDesigned for virtually every plant species

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NucleoSpin® Plant II (Mini / Midi / Maxi) Genomic DNA from plant and fungi samples

XX Rapid small, medium, and large scale preparation Choose the format to obtain as much DNA as you need

XX Alternative lysis buffers for optimal lysis Two alternative lysis buffers for optimal processing of various samples Lysis Buffer PL1 based on CTAB lysis method Lysis Buffer PL2 based on SDS lysis method

XX Improved buffer composition – get maximum yield and purity New buffer compositions allow optimized DNA yield and purity Isolation from a huge variety of plants

Product at a glance NucleoSpin® Plant II NucleoSpin® Plant II Midi NucleoSpin® Plant II Maxi

Technology Silica-membrane technology Silica-membrane technology Silica-membrane technologyFormat Mini spin columns Midi spin columns Maxi spin columnsSample material < 100 mg plant material (wet weight) < 400 mg plant material (wet weight) < 1 500 mg plant material (wet weight)

< 20 mg plant material (dry weight) < 80 mg plant material (dry weight) < 300 mg plant material (dry weight)Lysate clarification NucleoSpin® Filters NucleoSpin® Filters L NucleoSpin® Filters XLFragment size 50 bp – approx. 50 kbp 50 bp – approx. 50 kbp 50 bp – approx. 50 kbpTypical yield 1 – 30 μg 10 – 100 μg 50 – 300 μgA260/A280 1.8 – 1.9 1.8 – 1.9 1.8 – 1.9Elution volume 50 – 100 μL 200 – 400 μL 1 000 – 2 000 μLPreparation time 30 min / prep 90 min / prep 90 min / prepBinding capacity 50 μg 200 μg 500 μg

Application dataPurify DNA from a huge variety of plants

PCR analysis of isolated genomic DNA from different plant samples using NucleoSpin® Plant II. 100 mg plant material and Buffer PL1 or PL2 were used for lysis. PCR analysis was performed with 1 – 2 μL of each DNA eluate using primers against chloroplasts tRNA and loaded on a 1 % TAE agarose gel. Lane M: marker, lane 1 – 18: analyzed samples: willow, rose, fir needles, wheat, sugar beet, spruce needles, cherry tree, grass, elder bush, geranium, yew, stinging nettle, dragon tree, yellow soy bean, corn salad, garden cress, maize, gladiolus.

Alternative lysis – select the lysis buffer most suitable for your sample

Comparison to competitor kits. 100 mg fresh fir needles (Abies alba) were processed in triplicates using the NucleoSpin® Plant II kit (MN: Lysis Buffer PL1 and PL2) and competitor products (Q, P, and I). 10 μL of DNA eluates were analyzed on a 1 % TAE agarose gel.

M PL1 PL2 Q P I

M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18

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Application data Scale up your sample material – no compromise on purity or yield

Superior yield and purity DNA yield [μg] A260/A280 A260/A230 A: Isolation of genomic DNA from different plant samples using

NucleoSpin® Plant II Maxi in comparison to competitor Q. 1 500 mg of plant material were processed using Lysis Buffer PL1 and PL2 (NucleoSpin® Plant II Maxi) or competitor product Q. 10 μL of each DNA eluate was analyzed on a 1 % TAE agarose gel.

B: qPCR of DNA purified by NucleoSpin® Plant II Maxi. Primers against conserved 18S rRNA show reliable quality of the DNA isolated from willow, rose, fir needle, and sugar beet.

C: Tabular overview of yield and purity for all samples shown in figure A.

Willow MNQ

4611

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1.910.46

Rose MNQ

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1.620.43

Fir needle MNQ

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1.950.60

Wheat MNQ

232142

1.841.77

2.091.53

Sugar beet MNQ

3223

1.821.61

1.650.69

NucleoSpin® 8 / 96 Plant IIMedium- and high-throughput kits for genomic DNA isolation

XX Flexibility for alternating number of samples Flexible 8-well strip (1 – 6 in parallel) and proven 96-well format

XX Minimized risk of cross contamination MN Wash Plate included

XX Convenient handling Processing under vacuum or by centrifugation Manual and automated processing Scripts for most automation platforms available

NucleoMag® 96 PlantMagnetic-bead based isolation of genomic DNA

XX Magnetic solution for automated high throughput Easy resuspension, mixing, and efficient bead separation – minimized bead carry-over

XX Concentrated and ready-to-use DNA Small elution volumes ≥ 50 μL

XX Minimized risk of cross contamination One tube processing

Willow Rose Fir needle Wheat Sugar beet M PL1 PL2 Q PL1 PL2 Q PL1 PL2 Q PL1 PL2 Q PL1 PL2 Q M

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NucleoSpin® RNA Plant RNA from plant and fungi samples

XX Optimized lysis procedure Two alternative lysis buffers included – choose your buffer for best performance NucleoSpin® Filters (shredders) included for efficient homogenization

XX Get high RNA yield and outstanding purity Up to 70 μg ready-to-use RNA from 100 mg plant material RNA of high integrity and purity – no inhibition of RT-PCR

XX Suitable for a huge variety of plants Successful isolation of RNA from leaves, roots, seeds, stem, and many other plant materials

Application dataSuperior RNA quality from different plant species

A: Total RNA was isolated from 50 mg of different plant species using NucleoSpin® RNA Plant. Aliquots of each eluate were analyzed on a 1 % formaldehyde agarose gel (M: marker, lane 1: maize, 2: thyme, 3: castor oil plant, 4: tobacco, 5: rye)

B: Total RNA of 50 mg garden cress was run on an Agilent 2100 Bioanalyzer (RNA 6000 Nano Chip). The electropherogram docu-ments high structural integrity (lane 1: root, lane 2: leave).

Reliable removal of secondary metabolites

C: RT-PCR of total RNA isolated from different plant species using NucleoSpin® RNA Plant. 3 μL of total RNA eluate was used for RT-PCR reactions (30 cycles). Products were analyzed on a 1.2 % aga-rose gel (M: marker; lanes 1 – 2: tobacco, 3 – 4: tomato, 5 – 6: red pepper, 7 – 8: zucchini, 9 – 10: egg plant, 11 – 12: broccoli, 13 – 14: radish, 15 – 16: maize).

Ordering informationDNA ProductsSingle prep (spin columns) Preps REF

NucleoSpin® Plant II 10 / 50 / 250 740770.10 / .50 / .250

NucleoSpin® Plant II Midi 20 740771.20

NucleoSpin® Plant II Maxi 10 740772.10

Medium- and high-throughput (8-well strips and 96-well plates) Preps REF

NucleoSpin® 8 Plant II 12 x 8 / 60 x 8 740669 / .5

NucleoSpin® 8 Plant II Core Kit 48 x 8 740467.4

NucleoSpin® 96 Plant II 2 x 96 / 4 x 96 / 24 x 96 740663.2 / .4 / .24

NucleoSpin® 96 Plant II Core Kit 4 x 96 740468.4

NucleoMag® 96 Plant 1 x 96 / 4 x 96 / 24 x 96 744400.1 / .4 / .24

RNA ProductsSingle prep (spin columns) Preps REF

NucleoSpin® RNA Plant 10 / 50 / 250 740949.10 / .50 / .250

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Viral RNA / DNA purification products from MACHEREY-NAGEL ... · MACHEREY-NAGEL GmH Co G Neumann-Neander-Str 6-8 52355 Dren ermany Sierlan MACHEREY-NAGEL AG Tel 41 0 62 388 55 00