Embed Size (px)
Citation preview
Liquid Handling
Image Biomek FX Dual Bridge Liquid Handler(Manufacturer: Beckman Coulter)
This hybrid system provides maximum process flexibility.
Key Features
Dual pod system with a multichannel head (96 fixed channels) and a Span-8 (8 channels that can be programmed individually)
Automated plate moving using integrated gripper arm Dynamic pipetting range from 1 to 200 ul Handles 96- and 384-well plate formats, also pipettes
into test tubes, microfuge tubes, and troughs Deck capacity of 15 plus 3 (Span-8) ALPs (Automated
Labware Positioners) Orbital Shaker Positioned in temperature-controlled environment
Applications
o PCR and qPCR setupo PCR purificationo Cleanup of enzymatic reactions using magnetic bead
technology
o Genomic DNA purificationo RNA purificationo Plasmid DNA purificationo Nucleic acid quantification plate setup and
normalizationo Serial dilutionso Plate replication or dilutiono Aliquoting of reagents and biological samples,
generation of ready-to-use plateso Plate content reformatting (sorting)o Sample preparation for next-generation sequencing
applicationso Cherry pickingo Other high-speed and high-capacity automated
solutions
Liquid Handling
Image Biomek FX (single pod system)(Manufacturer: Beckman Coulter)
Key Features
Equipped with a single multichannel pod (96 fixed channels)
Automated plate moving using integrated gripper arm Dynamic pipetting range from 1 to 200 ul Handles 96- and 384-well plate formats Deck capacity of 19 ALPs (Automated Labware
Positioners)
Applications
o PCR and qPCR setupo PCR purificationo Cleanup of enzymatic reactions using magnetic bead
technologyo Genomic DNA purificationo RNA purificationo Plasmid DNA purificationo Nucleic acid quantification plate setup
o Serial dilutions o Plate replication or dilutiono Aliquoting of reagents and biological samples,
generation of ready-to-use plateso Sample preparation for next-generation sequencing
applicationso Plate setup for genome-wide siRNA and cDNA Library
Screens / Transfectiono Other high-speed and high-capacity automated
solutions
Liquid Handling
Image Biomek FXP (single pod system)(Manufacturer: Beckman Coulter)
The Biomek FXP is the latest entry in the Biomek line of robotic workstations. The updated hardware results in greater positional accuracy and increased robustness.
Key Features
Single pod system with one multichannel head (96 fixed channels)
Automated plate moving using integrated gripper arm Dynamic pipetting range from 1 to 200 ul Handles 96- and 384-well plate formats Deck capacity of 22 ALPs (Automated Labware
Positioners) Positioned in temperature-controlled environment
Applications
o PCR and qPCR setupo PCR purification
o Cleanup of enzymatic reactions using magnetic bead technology
o Genomic DNA purificationo RNA purificationo Plasmid DNA purificationo Nucleic acid quantification plate setupo Serial dilutionso Plate replication or dilutiono Aliquoting of reagents and biological samples,
generation of ready-to-use plateso Sample preparation for next-generation sequencing
applicationso Plate setup for genome-wide siRNA and cDNA Library
screens / Transfectiono Other high-speed and high-capacity automated
solutions
Liquid Handling
Image Biomek NXP Span-8(Manufacturer: Beckman Coulter)
Key Features
Single-pod system with one Span-8 (8 channels that can be programmed individually)
Dynamic pipetting range from 1 to 200 ul Handles 96- and 384-well plate formats, also pipettes
into test tubes, microfuge tubes, and troughs Deck capacity of 14 ALPs (Automated Labware
Positioners)
Applications
o PCR and qPCR setupo Nucleic acid quantification plate setup and
normalizationo Serial dilutionso Aliquoting of reagents and biological samples,
generation of ready-to-use plateso Plate content reformatting (sorting)o Cherry picking
Liquid Handling
Image JANUS Mini MDT Automated Workstation
(Manufacturer: Perkin Elmer)
Key Features
Single pod system with one multichannel head (96 fixed channels)
Automated plate moving using integrated gripper arm Dynamic pipetting range from 1 to 200 ul Handles 96- and 384-well plate formats Deck capacity of 11 ALPs (Automated Labware
Positioners) Sample tracking via barcode reader Orbital shaker (2) Vacuum manifold (2)
Applications
o PCR and qPCR setupo PCR purificationo Genomic DNA purificationo RNA purificationo Plasmid DNA purification
o Nucleic acid quantification plate setup and normalization
o Serial dilutionso Plate replication or dilutiono Aliquoting of reagents and biological samples,
generation of ready-to-use plates
Assay Readout
Image TopCount NXT(Manufacturer: Perkin Elmer)
The TopCount NXT is a fully contained benchtop microplate scintillation and luminescence counter. The system is specially designed and configured for direct scintillation and luminescence counting of samples contained in microplates and microsample tubes and provides maximum capability for both basic research and high-throughput screening.
Key Features
12-Detector: Simultaneous counting of twelve samples in 96-well and 384-well microplates
Automatic microplate stacker for 20 plate and 40 plate stacker cassettes for high unattended counting capacity
Fully automatic loading, counting, unloading, and repeat cycle counting of sample plate batches
High sensitivity for luminescence and liquid or solid scintillation counting
Prevention of optical cross-talk Isothermal counting chamber controls counting
chamber temperature to ensure a reproducible counting environment for all assays.
Automatic bar code label reader for positive sample identification
Provides automatic, assay-specific data processing for all user applications
Unattended, automatic spreadsheet analysis of count data to final results
Applications
o Cell proliferationo Receptor binding assayso Reporter gene assayso Solid Phase RIA/LIAo 51Cr release assayso HPLC fraction analysiso Scintillation Proximity Assays (SPA)
Assay Readout
Image SpectraMax Plus384 Absorbance Plate Reader
(Manufacturer: Molecular Devices)
Key Features
Reads a variety of sample formats from any standard cuvette to 96- and 384-well microplates in endpoint, kinetic, and spectral scan modes
Monochromator-based wavelength selection for UV/Visible absorbance between 190 nm and 1000 nm
Monitoring of temperature-sensitive reactions with consistent temperature regulation from ambient to 45°C
Correction for varying microplate well volumes and the elimination of standard curves
Thorough mixing of samples in microplate wells by user-programmable Automix feature
Applications
Essentially, all UV/VIS spectrophotometric assays that are read in a conventional spectrophotometer or microplate reader can be read in the SpectraMax Plus384 Reader:
o DNA quantitation o Colorimetric assays o ELISAs/EIAs o Immunoassays o Drug dissolution profiles o Enzyme kinetics (e.g., Ki, Km, etc.)
Assay Readout
Image SpectraMax Gemini EM (Manufacturer: Molecular Devices)
Key Features
Reads a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes.
Measures fluorescence from solutions or cell-based assays with the best optical sensitivity by switching between top and bottom fluorescence reading
Compares relative fluorescence units (RFUs) between samples, allowed by a unique calibration against an internal standard
Monitoring of temperature-sensitive reactions with consistent temperature regulation from ambient to 45°C
Thorough mixing of samples in microplate wells by user-programmable Automix feature
Gentle movement of cell or other aggregates in the microplate with a slow carriage speed option
Applications
The Gemini EM Microplate Reader supports a multitude of fluorescence assay categories including:
o ELISAs and Immunoassayso Nucleic Acid (DNA) Quantitationo Protein Quantitationo Reporter Gene Assayso Cell Viability, Proliferation, and Cytotoxicityo Enzyme Assayso Transporter Assayso Phosphotases/Kinaseso Microbial Growth
High-Throughput Colony Picking
Image RapidPick Complete Colony Picker
(Manufacturer: Hudson Robotics, Ic.)
The RapidPick Complete represents a fully-integrated solution for colony picking allowing automated selection and growth of cells grown on a colony plate. It also retains a record and image of the original colony and tracks it to a specific culture plate/well.
Key Features
Stand-alone enclosure with automated, multi-pin colony picker
Includes Hudson’s PlateCrane robot arm and stackers (PlateCrane can be configured with 2 to 10 stacks.)
Integrated robotic media dispenser and adhesive plate sealer to assure protection from outside contaminants
Picks colonies from SBS-compatible omni-trays, segmented colony plates, 24-well, 96-well and 384-well plates and petri dishes
Inoculates SBS-compatible omni-trays, 24-well, 96-well and 384-well deepwell and standard height plates.
Tracking of all sample movements
Applications
o High-throughput colony pickingo Colony replication, re-arraying, and archivingo Cherry picking of glycerol stocks
