Name ______________________________________________________________ Period __________ Date _______________________

Amoeba SistersDNA Replication: The Cell’s Extreme Team Sport

1. DNA replication means…

2. Where does DNA replication occur in eukaryotic cells?

3. When does DNA replication occur in eukaryotic cells?

4. Why is it important that DNA does NOT replicate during cell division?

5. The letters A-S-E in biology most likely denotes an

6. Helicase—the

enzyme.

7. DNA Polymerase—the

8. Primase—the

9. Ligase—the

10. In the DNA double helix, the two DNA strands C one another and are A

11. Number the carbons in each of the DNA strands pictured to help understand the 5’ to 3’ ends.

12. Place arrows to show the 5’ to 3’ direction on both strands.

13. Label the leading strand and lagging strands.

14.

Explain why there are multiple primers on the lagging strand but only one on the leading strand.

15. Name the short fragments that are created on the lagging strand.

16. What enzyme is responsible for “gluing” the short fragments together?

17. Summarize the steps of DNA replication using one of the enzymes in each step. (Hint: 4 steps can get the job done.) Remember, you can stop and rewind the video if necessary.

18. DNA replication is called semi-conservative. Explain why.

19. Look back at the title of this video. Why did the Amoeba Sisters choose it?