· Web view10 Lactose is synthesised in the Golgi apparatus and transported in vesicles through the cytoplasm. Because lactose is unable to escape from these vesicles, they increase

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Q1. Read the following passage.

Human milk contains all the nutrients a young baby needs in exactly the rightproportions. It is formed in the mammary glands by small groups of milk-producing cells. These cells absorb substances from the blood and use them to synthesise the lipids, carbohydrates and proteins found in milk. Milk-producing cells are roughly cube-shaped

5 and have a height to breadth ratio of approximately 1.2 : 1.

The main carbohydrate in milk is lactose. Lactose is a disaccharide formed by the condensation of two monosaccharides, glucose and galactose. (A molecule of galactose has the same formula as a molecule of glucose – the atoms are just arranged in a different way.)

10 Lactose is synthesised in the Golgi apparatus and transported in vesicles through the cytoplasm. Because lactose is unable to escape from these vesicles, they increase in diameter as they move towards the plasma membrane. The vesicle membranes fuse with the plasma membrane and the vesicles empty their contents out of the cell.

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Use the information from the passage and your own knowledge to answer the following questions.

(a) (i) The breadth of a milk-producing cell is 26 µm. Calculate the height of this cell.

Height = .......................... µm(1)

(ii) Describe and explain how you would expect the height to breadth ratio of an epithelial cell from a lung alveolus to differ from the height to breadth ratio of a milk-producing cell.

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(b) How many oxygen atoms are there in a molecule of

(i) galactose;

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(ii) lactose?

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(c) The lactose-containing vesicles increase in diameter as they move towards the plasma membrane of the milk-producing cell (lines 11-12). Use your knowledge of water potential to explain why.

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(d) Suggest one advantage of milk-producing cells containing large numbers of mitochondria.

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(e) Some substances pass through the plasma membrane of a milk-producing cell by diffusion. Describe the structure of a plasma membrane and explain how different substances are able to pass through the membrane by diffusion.

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(Total 15 marks)

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An anti-gal antibody is a type of antibody that helps to fight infections causedby bacteria. If a person has a bacterial infection, for example Salmonella, anti-galantibodies bind to antigens on the surface of the Salmonella. Not all theanti-gal antibodies are used to fight the infection. Even after the infection, anti-galantibodies remain in the blood. 5

Scientists have made adaptor molecules to try to use the anti-gal antibodiesagainst viruses such as HIV. The adaptor molecules are proteins. Each adaptormolecule had a receptor site to which the HIV binds. This receptor site wassimilar to the receptor site on human cells to which the HIV binds. Theadaptor molecule has another site to which an anti-gal antibody will bind. 10

The scientists then investigated whether adding adaptor molecules and anti-galantibodies can prevent HIV entering cells. They added adaptor moleculesand anti-gal antibodies to a culture of human cells. They then added HIVto the culture. Their results showed that 90% of the virus particles failedto infect cells. 15

The scientists are hoping to develop a different type of adaptor molecule to use against MRSA.

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(a) (i) What is an antigen? (line 3)

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(ii) Explain why antibodies against Salmonella do not normally bind to HIV.

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(iii) Explain how the adaptor molecule allows anti-gal antibodies to associate with HIV.

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(b) Describe how humans produce antibodies against a pathogen such as Salmonella.

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(c) (i) HIV infects some human cells, such as T-cells, but not others. Suggest why.

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(ii) Antibiotics are not used to treat viral infections, such as HIV. Explain why.

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(d) (i) When HIV, anti-gal and the adaptor molecule were added to a culture of human cells, 90% of the virus did not infect human cells. (lines 12-15). Explain why.

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(ii) Explain why a different type of adaptor molecule will have to be made to use against MRSA. (lines 16-17)

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(Total 20 marks)


Gluten is a protein found in wheat. When gluten is digested in the small intestine,the products include peptides. Peptides are short chains of amino acids. Thesepeptides cannot be absorbed by facilitated diffusion and leave the gut in faeces

Some people have coeliac disease. The epithelial cells of people with coeliac diseasedo not absorb the products of digestion very well. In these people, some of the 5 peptides from gluten can pass between the epithelial cells lining the small intestineand enter the intestine wall. Here, the peptides cause an immune response that leadsto the destruction of microvilli on the epithelial cells.

Scientists have identified a drug which might help people with coeliac disease.It reduces the movement of peptides between epithelial cells. They have 10carried out trials of the drug with patients with coeliac disease.

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Use the information in the passage and your own knowledge to answer the following questions.

(a) Name the type of chemical reaction which produces amino acids from proteins.

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(b) The peptides released when gluten is digested cannot be absorbed by facilitated diffusion (lines 2 – 3). Suggest why.

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(c) Explain why the peptides cause an immune response (lines 7 – 8).

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(d) Scientists have carried out trials of a drug to treat coeliac disease (lines 10 – 11).Suggest two factors that should be considered before the drug can be used on patients with the disease.

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(Total 7 marks)

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Q4. In taxonomy, each of the levels of classification (class, family, genus, kingdom, order, phylum and species) is called a taxon. The diagram represents just three of these levels of classification.

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Explain which of these levels of classification could not be

(i) a genus;........................................................................................................

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(ii) a phylum.......................................................................................................

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The plasma membrane plays a vital role in microorganisms. It forms a barrier between the celland its environment, controlling the entry and exit of solutes. This makes bacteria vulnerableto a range of antiseptics and antibiotics

When bacteria are treated with antiseptics, the antiseptics bind to the proteins in the

5 membrane and create tiny holes. Bacteria contain potassium ions at a concentration many

times that outside the cell. Because of the small size of these ions and their concentration inthe cell, the first observable sign of antiseptic damage to the plasma membrane is the leakingof potassium ions from the cell. Some antibiotics damage the plasma membrane in a similarway. One of these is tyrocidin. This is a cyclic polypeptide consisting of a ring of ten amino

10 acids. Tyrocidin and other polypeptide antibiotics are of little use in medicine.

Other antibiotics also increase the rate of potassium movement from cells. It is thought thatpotassium ions are very important in energy release and protein synthesis, and a loss ofpotassium ions would lead to cell death. Gramicidin A coils to form a permanent pore passingthrough the plasma membrane. This pore enables potassium ions to be conducted from the

15 inside of the cell into the surrounding medium. Vanilomycin also facilitates the passage of

potassium ions from the cell. A molecule of vanilomycin forms a complex with a potassiumion and transports it across the membrane. The potassium ion is released on the outside andthe vanilomycin is free to return and pick up another potassium ion. Vanilomycin depends onthe fluid nature of the plasma membrane in order to function.

20 Polyene antibiotics have flattened ring-shaped molecules. The two sides of the ring differ from

each other. One side consists of an unsaturated carbon chain. This part is stronglyhydrophobic and rigid. The opposite side is a flexible, strongly hydrophilic region. It has beenshown that polyene antibiotics bind only to sterols. Sterols are lipids found in the membranesof eukaryotes but not in the membranes of prokaryotic organisms. It is thought that several

25 sterol-polyene complexes come together. The plasma membranes of eukaryotic cells treated

with these polyene antibiotics lose the ability to act as selective barriers and small ions andmolecules rapidly leak out

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Use information in the passage and your own knowledge to answer the questions.

(a) By what process do potassium ions normally enter a bacterial cell? Explain the evidence for your answer.

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(b) (i) Draw a peptide bond showing how the COOH group of one amino acid joins to the NH2 group of another.

(1)

(ii) How many peptide bonds are there in a molecule of tyrocidin (lines 9 - 10)?

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(c) Experiments have shown that vanilomycin is unable to transport potassium ions across a membrane when it is cooled. Gramicidin A continues to facilitate the movement of potassium ions at these low temperatures. Explain these results.

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(d) Draw a simple diagram of one of the phospholipid layers to show how polyene antibiotics allow small ions and molecules to leak rapidly through a plasma membrane. Use the following symbols to represent the different molecules.

Note that the zigzag line on the symbol for the polyene antibiotic represents its hydrophobic region.

(2)

(Total 9 marks)

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Q6. There is evidence that the first photosynthetic organisms were primitive water-dwelling bacteria. The very first of these lived near the surface of the water in lakes and contained a purple pigment that absorbed light most strongly in the green region of the spectrum. Later, other bacteria evolved that lived on the top of sediment at the bottom of the lakes (Figure 1). Gene mutations had enabled these bacteria to synthesise chlorophyll instead of the purple pigment present in the bacteria living near to the surface. Chlorophyll absorbs light most strongly in the blue and red regions of the spectrum (Figure 2).

Figure 1

Figure 2

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(a) Describe how light energy absorbed by chlorophyll molecules is used to synthesise ATP.

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(b) Use Figure 2 to explain how natural selection would favour the evolution of sediment-dwelling bacteria containing a different photosynthetic pigment from those living near the surface of the water.

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(Total 11 marks)

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M1. (a) (i) 31 / 31.2;1

(ii) Ratio would be less / smaller;Cell is thin / has large surface area / (adapted) for diffusion;

Accept converse. Must relate to concept of ratio.2

(b) (i) 6;1

(ii) 11;1

(c) Water potential inside vesicle more negative / lower; Water moves into vesicle by osmosis / diffusion;

2

(d) Mitochondria supply energy / ATP;For active transport / absorption against concentrationgradient / synthesis / anabolism / exocytosis / pinocytosis;

Do not credit references to making,creating or producing energy.

2

(e) 1 Phospholipids forming bilayer / two layers;2 Details of arrangement with “heads” on the outside;3 Two types of protein specified; e.g. passing right through or confined to one layer / extrinsic or intrinsic / channel proteins and carrier proteins / two functional types4 Reference to other molecule e.g. cholesterol or glycoprotein;5 Substances move down concentration gradient / from high to low concentration;

Reject references to across or along a gradient6 Water / ions through channel proteins / pores;7 Small / lipid soluble molecules / examples pass between phospholipids /

through phospholipid layer;8 Carrier proteins involved with facilitated diffusion;

Ignore references to active transport.Credit information in diagrams.

max 6[15]

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M2. (a) (i) Molecule/protein/glycoprotein;Stimulates immune response;(That causes) production of antibodies;

2 max

(ii) Antigens on HIV are different (shape);So, antibody will not ‘fit’/not complementary (to antigen);Receptor sites on antibody specific to one antigen;

2 max

(iii) (Has site with) same shape as salmonella antigen so bindsto anti-gal antibodies;(Has site with) same shape as receptor molecule so that HIV will bind;Binds to both molecules;

2 max

(b) Salmonella pathogen has specific antigen on surface;Salmonella pathogen engulfed by macrophage;T-cells activate B-cells;B-cell with complementary/specific receptor antibody activated/clonal selection;B-cells divide/form clone/clonal expansion;Plasma cells make antibodies;Specific to antigen/bind to salmonella bacterial antigen;

Accept macrophage presents antigen to T/B cells;Accept T-cells release factors;

6 max

(c) (i) HIV binds to specific receptor;Only present on certain cells / T-cells;

2

(ii) Antibiotics stop metabolism, viruses don’t have metabolism;Viruses hide in cells, antibiotics can’t reach;

Two suitable cell components antibiotics work against thatviruses don’t have;e.g. some antibiotics work against ribosomes, that viruses don’t have

2

(d) (i) Adaptor molecule binds to HIV;(This) prevents the HIV binding to the receptor;

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Therefore few HIV available to infect cells;2 max

(ii) Would need to be complementary to MRSA (antigens);MRSA has different antigens;But would still need to have binding site for anti-gal;

2 max[20]

M3. (a) Hydrolysis (reaction);Accept phonetic spelling

1

(b) 1. Too big / wrong shape;Wrong charge - neutralAccept insoluble

2. To fit / bind / pass through (membrane / into cell / through carrier / channel protein);

3. Carrier / channel protein;Accept carrier / channel protein not present

3

(c) Foreign / (act as) antigen / non-self;Reject foreign cells

1

(d) 1. Dose to be given;Accept: interaction with other drugs

2. No (serious) side effects;

3. How effective;

4. Cost of drug;2 max

[7]

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M4. (i) Taxon A - there is more than one level / taxon below it / genus only has species / only has one level / taxon above it;

(ii) Taxon C - there is more than one level / taxon above it / phylum only has kingdom / only has one level taxon above it;

[2]

M5. (a) Rate of movement / diffusion proportional to concentration gradient / difference in concentration;High concentration of potassium ions inside cell compared to outside;Must mention high concentration. Ignore reference to other factors ifreasoning is appropriate.

2

(b) (i) O || C – N | H;

1

(ii) 10;1

(c) Action of vanilomycin depends on fluidity of membrane;Fluidity reduced / not fluid at low temperatures;Pore formed by gramicidin A remains in place / permanent;

3

(d) Pore between sterol molecules lined with polyene antibiotic;Hydrophobic region next to sterol;

2[9]

M6. (a) Excitation of chlorophyll molecule / electrons / energy of (pairs of)electrons raised to higher energy level;

Electron(s) emitted from chlorophyll molecule;

Electron(s) to electron transport chain;

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Loss of energy by electron(s) along electron transport chain;

Energy lost by electron(s) is used to synthesise ATP;

From ADP + Pi;“By electrons” need not be stated in each marking point if it can be reasonably inferred that the candidate is referring to electrons

max 5

(b) Little green light reaches bottom as absorbed by surface dwellers / water;Red and blue not absorbed and so penetrate;Variation in pigments of sediment dwellers;Bacteria with chlorophyll at an advantage as chlorophyll absorbs red and blue;(Survive to) reproduce in greater numbers and pass on advantageous alleles / genes in greater numbers / increase in frequency of advantageousalleles in subsequent generations;Increase in frequency / numbers of bacteria with chlorophyll;

6[11]

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Documents

· Web view10 Lactose is synthesised in the Golgi apparatus and transported in vesicles through the cytoplasm. Because lactose is unable to escape from these vesicles, they increase