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S52 Abstracts / Journal of Biotechnology 185S (2014) S37–S125 Canine degenerative myelopathy Zuzana Firdova 1,, Evelina Turnova 1 , Marcela Bielikova 2 , Jan Turna 1 , Andrej Dudas 2 1 Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, Mlynská dolina, 842 15 Bratislava, Slovak Republic 2 Slovgen s.r.o., Dúbravská cesta 21, 841 04 Bratislava 4, Slovak Republic E-mail address: z.fi[email protected] (Z. Firdova). Degenerative myelopathy (DM) belongs to the neurodegenerative diseases. DM leads to coordination deficits – lower body ataxia, dis- ruption of sensitivity and reflexes, loss and lack of coordination. DM is a progressive, non-inflammatory, painless degeneration of the myelin sheath of spinal nerves and the white matter of the spinal cord. It leads to disruption of communication pathways between the brain and the spinal cord. To date, 43 dog breeds are known to be affected. The cause of DM is an autosomal recessive muta- tion in gene SOD1 with incomplete penetrance. Gene SOD1 encodes superoxide-dismutase 1. SOD1 gene (462 bp) is localized on chro- mosome 31 and encodes 153 amino acid residues. The defect was identified in exon 2 as a c.118G>A transition that results in an E40K missense mutation which involves a toxic gain of the function which changes protein. Our goal was to diagnose the mutation of 1726 individuals of 15 commonly affected purebreds and to extract a statistical overview summarizing the incidence of the prevalence of each inherited disease within the populations. It was based on PCR, restriction analysis and the separation of fragments via poly- acrylamide gel electrophoresis. The most affected breeds were the Welsh Corgi Pembroke and the Rhodesian ridgeback. http://dx.doi.org/10.1016/j.jbiotec.2014.07.174 Study regarding births on sows maintained in different types of stalls Cosmina Chirila , Ioan Petroman, Cornelia Petroman, Diana Marin, Carmen Dumitrescu, Cristian Bejan, Bogdan Momir Banat University of Agricultural Sciences and Veterinary Medicine “King Michael of Romania” Timisoara, Romania E-mail address: [email protected] (C. Chirila). Birth on sows in gestation maintained in stalls of 9 square meters with a capacity of 8 heads towards the ones maintained in stalls of 16 heads or 32 heads were not registered statistically significant differences (test x 2 p < 0.05). Between animals maintained in gesta- tion in stalls with capacity of 16 sows and 32 sows were statistically significant differences (test x 2 p > 0.05) the best results regarding birth in case of maintenance during gestation period in stalls of 9 square meters are obtained at sows maintained in stalls of 8 heads 84.07 ± 2.41. Birth at animals maintained in individual stalls was 83.06 ± 1.49 and for stalls of 9 square meters of 81.53 ± 1.77, the difference being statistically significant x 2 p < 0.05 test. http://dx.doi.org/10.1016/j.jbiotec.2014.07.175 Values of some reproduction indicators in grazed sows depending on artificial insemination number and intervals Cristian Bejan , Ioan Petroman, Tiberiu Iancu, Cornelia Petroman, Florin Daniel Bogosel, Diana Marin, Ramona Ciolac, Carmen Dumitrescu, Cosmina Chirila Banat University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” Timisoara, Romania E-mail address: [email protected] (C. Bejan). The values of some reproduction indicators in grazed sows varies depending on the interval between artificial inseminations and on the number of artificial inseminations: thus, fecundity 28 days after insemination in the studied sows was 86.43 ± 2.40 for the sows inseminated twice at a 15 h interval, 87.40 ± 2.11 for the sows inseminated twice at a 20 h interval and 90.24 ± 2.98 for the sows inseminated thrice; the difference was very significant statistically between the sows inseminated twice and the sows inseminated thrice with the test x 2 p > 0.001. Fifty-six days after the insemina- tion, the difference was very significant p > 0.001 from the point of view of the fecundity; the sows inseminated thrice recording the best indicator. Birth rate was higher in the sows inseminated thrice compared with the sows inseminated twice at different time inter- vals, with a significant statistic difference (x 2 p > 0.05). Prolificacy was not higher in the sows inseminated thrice than in the sows inseminated twice, with a difference that was not statistically sig- nificant (x 2 p > 0.05); the number of piglets still born did not record statistically significant differences, with x 2 p > 0.05, depending on the time interval between inseminations and on the number of inseminations. http://dx.doi.org/10.1016/j.jbiotec.2014.07.176 Biocatalysis/Biotransformation Co-assimilation of cellobiose and xylooligosaccharides using E. coli displaying both beta-glucosidase and beta-xylosidase on its cell surface Tsutomu Tanaka , Mariko Nakano, Yuuki Hirata, Akihiko Kondo Department of Chemical Science and Engineering, Kobe University, Kobe, Japan E-mail address: [email protected] (T. Tanaka). We constructed both beta-glucosidase (BGL) – and beta-xylosidase (XYL) – displaying Escherichia coli strain. After screening active XYLs, BSU17580 or Tfu1616, which are XYL from Bacillus sub- tilis or Thermobifida fusca YX, was successfully displayed on the E. coli cell surface using Blc or HdeD as an anchor protein and directly assimilated xylooligosaccharides as a carbon source. BGL was also successfully displayed in the E. coli cell surface using Blc as an anchor protein. The BGL- and XYL-displaying E. coli was successfully grown on 1% cellobiose and 1% xylooligosaccharides mixture, and the O.D. 600 was 1.76 after 10 h cultivation, which was higher and faster than that grown on glucose/xylose mixture (1.20 after 12 h cultivation). In addition, we demonstrated direct cadav- erine production from xylooligosaccharides and cellobiose using cellulase-displaying E. coli. http://dx.doi.org/10.1016/j.jbiotec.2014.07.177

Values of some reproduction indicators in grazed sows depending on artificial insemination number and intervals

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Page 1: Values of some reproduction indicators in grazed sows depending on artificial insemination number and intervals

S52 Abstracts / Journal of Biotechnology 185S (2014) S37–S125

Canine degenerative myelopathy

Zuzana Firdova 1,∗, Evelina Turnova 1, MarcelaBielikova 2, Jan Turna 1, Andrej Dudas 2

1 Department of Molecular Biology, Faculty ofNatural Sciences, Comenius University, Mlynskádolina, 842 15 Bratislava, Slovak Republic2 Slovgen s.r.o., Dúbravská cesta 21, 841 04Bratislava 4, Slovak Republic

E-mail address: [email protected] (Z. Firdova).

Degenerative myelopathy (DM) belongs to the neurodegenerativediseases. DM leads to coordination deficits – lower body ataxia, dis-ruption of sensitivity and reflexes, loss and lack of coordination. DMis a progressive, non-inflammatory, painless degeneration of themyelin sheath of spinal nerves and the white matter of the spinalcord. It leads to disruption of communication pathways betweenthe brain and the spinal cord. To date, 43 dog breeds are knownto be affected. The cause of DM is an autosomal recessive muta-tion in gene SOD1 with incomplete penetrance. Gene SOD1 encodessuperoxide-dismutase 1. SOD1 gene (462 bp) is localized on chro-mosome 31 and encodes 153 amino acid residues. The defect wasidentified in exon 2 as a c.118G>A transition that results in anE40K missense mutation which involves a toxic gain of the functionwhich changes protein. Our goal was to diagnose the mutation of1726 individuals of 15 commonly affected purebreds and to extracta statistical overview summarizing the incidence of the prevalenceof each inherited disease within the populations. It was based onPCR, restriction analysis and the separation of fragments via poly-acrylamide gel electrophoresis. The most affected breeds were theWelsh Corgi Pembroke and the Rhodesian ridgeback.

http://dx.doi.org/10.1016/j.jbiotec.2014.07.174

Study regarding births on sows maintained indifferent types of stalls

Cosmina Chirila ∗, Ioan Petroman, CorneliaPetroman, Diana Marin, Carmen Dumitrescu,Cristian Bejan, Bogdan Momir

Banat University of Agricultural Sciences andVeterinary Medicine “King Michael of Romania”Timisoara, Romania

E-mail address: [email protected] (C. Chirila).

Birth on sows in gestation maintained in stalls of 9 square meterswith a capacity of 8 heads towards the ones maintained in stallsof 16 heads or 32 heads were not registered statistically significantdifferences (test x2p < 0.05). Between animals maintained in gesta-tion in stalls with capacity of 16 sows and 32 sows were statisticallysignificant differences (test x2p > 0.05) the best results regardingbirth in case of maintenance during gestation period in stalls of 9square meters are obtained at sows maintained in stalls of 8 heads84.07 ± 2.41. Birth at animals maintained in individual stalls was83.06 ± 1.49 and for stalls of 9 square meters of 81.53 ± 1.77, thedifference being statistically significant x2p < 0.05 test.

http://dx.doi.org/10.1016/j.jbiotec.2014.07.175

Values of some reproduction indicators ingrazed sows depending on artificialinsemination number and intervals

Cristian Bejan ∗, Ioan Petroman, Tiberiu Iancu,Cornelia Petroman, Florin Daniel Bogosel, DianaMarin, Ramona Ciolac, Carmen Dumitrescu,Cosmina Chirila

Banat University of Agricultural Sciences andVeterinary Medicine “King Michael I of Romania”Timisoara, Romania

E-mail address: [email protected] (C. Bejan).

The values of some reproduction indicators in grazed sows variesdepending on the interval between artificial inseminations andon the number of artificial inseminations: thus, fecundity 28 daysafter insemination in the studied sows was 86.43 ± 2.40 for thesows inseminated twice at a 15 h interval, 87.40 ± 2.11 for the sowsinseminated twice at a 20 h interval and 90.24 ± 2.98 for the sowsinseminated thrice; the difference was very significant statisticallybetween the sows inseminated twice and the sows inseminatedthrice with the test x2p > 0.001. Fifty-six days after the insemina-tion, the difference was very significant p > 0.001 from the point ofview of the fecundity; the sows inseminated thrice recording thebest indicator. Birth rate was higher in the sows inseminated thricecompared with the sows inseminated twice at different time inter-vals, with a significant statistic difference (x2p > 0.05). Prolificacywas not higher in the sows inseminated thrice than in the sowsinseminated twice, with a difference that was not statistically sig-nificant (x2p > 0.05); the number of piglets still born did not recordstatistically significant differences, with x2p > 0.05, depending onthe time interval between inseminations and on the number ofinseminations.

http://dx.doi.org/10.1016/j.jbiotec.2014.07.176

Biocatalysis/Biotransformation

Co-assimilation of cellobiose andxylooligosaccharides using E. coli displayingboth beta-glucosidase and beta-xylosidase onits cell surface

Tsutomu Tanaka ∗, Mariko Nakano, Yuuki Hirata,Akihiko Kondo

Department of Chemical Science and Engineering,Kobe University, Kobe, Japan

E-mail address: [email protected] (T. Tanaka).

We constructed both beta-glucosidase (BGL) – and beta-xylosidase(XYL) – displaying Escherichia coli strain. After screening activeXYLs, BSU17580 or Tfu1616, which are XYL from Bacillus sub-tilis or Thermobifida fusca YX, was successfully displayed on theE. coli cell surface using Blc or HdeD as an anchor protein anddirectly assimilated xylooligosaccharides as a carbon source. BGLwas also successfully displayed in the E. coli cell surface usingBlc as an anchor protein. The BGL- and XYL-displaying E. coli wassuccessfully grown on 1% cellobiose and 1% xylooligosaccharidesmixture, and the O.D. 600 was 1.76 after 10 h cultivation, which washigher and faster than that grown on glucose/xylose mixture (1.20after 12 h cultivation). In addition, we demonstrated direct cadav-erine production from xylooligosaccharides and cellobiose usingcellulase-displaying E. coli.

http://dx.doi.org/10.1016/j.jbiotec.2014.07.177