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Validated LC-MS determination of hepcidin in human plasma. A new method for the determination of hepcidin peptide using LC-high resolution-MS has been fully validated. The article describing the method is in preparation. LC-HR-MS chromatogram. - PowerPoint PPT Presentation
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C:\XCALIBUR\...\130115_7 15.01.2013 14:30:49 Hepcidin_0nM_Pig
RT: 3.10 - 3.70 SM: 5B
3.10 3.15 3.20 3.25 3.30 3.35 3.40 3.45 3.50 3.55 3.60 3.65 3.70Time (min)
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RT: 3.42AA: 7564SN: 9
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3.403.263.243.22
3.143.35
RT: 3.42MA: 1163785SN: INF
3.573.553.533.36
NL: 1.50E4m/z= 558.60751-558.61869+558.80818-558.81936+559.00667-559.01785 F: FTMS + p ESI Full ms [500.00-1000.00] MS ICIS 130115_7
NL: 4.00E5m/z= 562.61638-562.62764+562.81690-562.82816+563.01659-563.02785 F: FTMS + p ESI Full ms [500.00-1000.00] MS 130115_7
C:\XCALIBUR\...\130115_8 15.01.2013 14:44:56 Hepcidin_025nM_Pig
RT: 3.10 - 3.70 SM: 5B
3.10 3.15 3.20 3.25 3.30 3.35 3.40 3.45 3.50 3.55 3.60 3.65 3.70Time (min)
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RT: 3.42AA: 41718SN: 31
RT: 3.42MA: 1064979SN: INF
3.663.55
3.313.293.13 3.21
NL: 1.50E4m/z= 558.60751-558.61869+558.80818-558.81936+559.00667-559.01785 F: FTMS + p ESI Full ms [500.00-1000.00] MS ICIS 130115_8
NL: 4.00E5m/z= 562.61638-562.62764+562.81690-562.82816+563.01659-563.02785 F: FTMS + p ESI Full ms [500.00-1000.00] MS 130115_8
C:\XCALIBUR\...\130115_48 16.01.2013 00:11:00 Hepcidin_50nM_E_Pig
RT: 3.10 - 3.70 SM: 5B
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RT: 3.43AA: 37890769SN: 15325
3.663.553.333.253.233.12RT: 3.43AA: 4101522SN: 16432
3.54 3.653.333.10 3.21 3.303.26
NL: 1.15E7m/z= 558.60751-558.61869+558.80818-558.81936+559.00667-559.01785 F: FTMS + p ESI Full ms [500.00-1000.00] MS ICIS 130115_48
NL: 1.28E6m/z= 562.61638-562.62764+562.81690-562.82816+563.01659-563.02785 F: FTMS + p ESI Full ms [500.00-1000.00] MS ICIS 130115_48
Validated LC-MS determination of hepcidin in human plasma
Hepcidin 0.25nM
Hepcidininternal standard
Blank matrixPork plasma
Hepcidininternal standard
Hepcidin 50nM
Hepcidininternal standard
LC-HR-MS chromatogram
LC-HR-MS chromatogram LC-HR-MS chromatogram
0 1 2 3 4 50.0
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ZOOMED
Hepcidin_Z5Y = -0.010456+0.13502*X-0.000355996*X^2 R^2 = 0.9904 W: 1/X^2
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Hepcidin nM
Calibration Curve ; Limit of quantification = 0.25nM = 0.75ng/mL
A new method for the determination of hepcidin peptide using LC-high resolution-MS has been fully validated. The article describing the method is in preparation.
