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Vaccine War
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“Undergraduate Research Opportunities” will be held Today, Thursday, Jan 13, at 3:30 pm in SL120
•SEMINARS!!!!
The Weiss Laboratory pursues both chemical and biological aspects of chemical biology. Using chemistry to advance a molecular understanding of biology, the lab dissects key events in biology with exceptionally diverse combinatorial libraries as atomic-scale scalpels. Our libraries, collections of different molecules, include virus-displayed proteins and chemically or enzymatically synthesized small molecules. Libraries displayed on the surfaces of viruses also offer essentially universal molecular recognition for chemical sensors, illustrating how biology can advance chemistry.
Prof. Gregory Weiss, Department of Chemistry, University of California, Irvine
Works exactly like HK.
Activated by [AMP] even in the presence of hi [ATP].
Inhibited by hi [ATP]or citrate
Figure 17-8 Mechanism for base-catalyzed aldol cleavage.
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Transition state analogs like 2-phosphoglycolate inhibit the enzyme
CH2OP
OO_
Figure 17-9 Enzymatic
mechanism of Class I aldolase.
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OH
NHH
CH2OP
14Lys
Enzyme-Substrate Complex trapped by reduction of DHAP with NaBH4 followed by hydrolysis
Figure 16-10 Mechanism of aldose–ketose isomerization.
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Figure 17-10 Proposed enzymatic mechanism of the TPI reaction: General Acid Catalysis.
pKs = 6.5 and 9.5Like PGIBut pK1 is for GLU! Normal pk?
GluAsp activity by 1000!
Reaction rate is diffusion limited!!
4.1
End of Glycolysis Collection Phase
• Net result so far?– ATP– NAD+
– Carbon
Start of energy producing phase of glycolysis: Production of the first hi energy molecule.
Figure 17-13a Some reactions employed in elucidating the enzymatic mechanism of GAPDH. (a) The reaction of iodoacetate with an active site Cys residue. (b) Quantitative tritium transfer from substrate to NAD+.
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32Pi also incorporated
Figure 17-14 Enzymatic mechanism of glyceraldehyde-3 phosphate dehydrogenase.
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6 Go’ = +6.7 kJ!
Figure 17-15 Space-filling model of yeast phosphoglycerate kinase showing its deeply
clefted bilobal structure.
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Figure 17-16 Mechanism of the PGK reaction.P
age
597
Go’ = -49.4 kJ! Go’ = -12.1 kJ
Mutases move functional groups: 3PG2PG
Animated mechanism
Figure 17-19 The pathway for the synthesis and degradation of 2,3-BPG in erythrocytes is a detour
from the glycolytic pathway.
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Figure 17-20 The oxygen-saturation curves of hemoglobin (red) in normal
erythrocytes and those from patients with hexokinase (green) and pyruvate kinase
deficiencies (purple).
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BPG
BPG
Figure 17-18
Proposed reaction
mechanism for
phospho-glycerate mutase.
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Phosphorylatedactive site
Bisphospho-intermediate.
Figure 17-22 Mechanism of the reaction catalyzed by
pyruvate kinase.
Let's sing!!
• http://www.csulb.edu/~cohlberg/songbook.html
Lyrics
http://books.google.com/books?id=oq9ENyL_d9YC&lpg=PP1&pg=PA1#v=onepage&q&f=false
Figure 17-21 Proposed reaction mechanism of enolase.
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F- binds Pi + Mg+2
Potent inhibitor
Figure 17-23 The active site region of porcine H4 LDH in complex with S-lac-NAD+, a
covalent adduct of lactate and NAD+.
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Figure 17-24 Reaction mechanism of lactate dehydrogenase.
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Figure 17-25 The two reactions of alcoholic
fermentation.
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Figure 17-26 Thiamine pyrophosphate.
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Figure 17-27
Reaction mechanism of pyruvate
decarboxylase.
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Figure 17-29 The formation of the active ylid form of TPP in the
pyruvate decarboxylase
reaction.
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Figure 17-30The reaction mechanism of alcohol dehydrogenase involves direct hydride transfer of the pro-R hydrogen of NADH to the re face of acetaldehyde.
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Table 17-2 Some Effectors of the Nonequilibrium Enzymes of Glycolysis.
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Figure 17-32a X-Ray structure of PFK. (a) A ribbon diagram showing two subunits of the
tetrameric E. coli protein.
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Mg+2
ATP
F6P
Figure 17-33 PFK activity versus F6P concentration.
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Figure 17-35 Metabolism of fructose.
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Figure 17-36 Metabolism of galactose.
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Figure 17-37 Metabolism of mannose.
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Figure 17-31 Schematic diagram of the plasmid constructed to control the amount of
citrate synthase produced by E. coli.
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“Alfonse, Biochemistry makes my head hurt!!”\
