V Cadot,: WP meeting Leafy Vegetables 27th September 2007 WP meeting Leafy Vegetables WP 3 Cadot Valérie GEVES : Variety and seed, study and control group

V Cadot,: WP meeting Leafy Vegetables 27th September 2007

WP meeting Leafy Vegetables WP meeting Leafy Vegetables

WP 3WP 3Cadot Valérie

GEVES :Variety and seed, study and control group

Groupe d’Etude et de contrôle des Variétés Et des Semences


A.A. ObjectivesObjectives

Evaluation for pests and diseases resistanceEvaluation for pests and diseases resistance

Evaluation for quality and abiotic charactersEvaluation for quality and abiotic characters

Inclusion in the databasesInclusion in the databases

to enable a more sustainable production methods and a to enable a more sustainable production methods and a

better quality productbetter quality product


DeliverablesDeliverables for WP3 for WP3Deliverable dateWP3

Deliverable descriptionStatus Partner

responsible

Year 1

D16; month 12

Seeds of the selected material of the four crop groups sent to the partners involved

On going P0, P1, P2, P3, P4, P5, P7, P8, P9, P10, P12, P13

Year 4

D17; month 45 115 accessions of lettuce evaluatedNot yet due P1, P4, P7,

P9, P10, P13

D18; month 45 320 accessions of spinach evaluated Not yet due P0, P5

D19; month 45 265 accessions of chicory evaluated Not yet due P2, P8, P12

D20; month 45 49 accessions of minor crops evaluatedNot yet due P3, P5, P9,

P12

D21; month 45Evaluation data entered in a fixed format to

be sent to the database managers

Not yet due P0, P1, P2, P3, P4, P5, P7, P8, P9, P10, P12, P13


Milestones for WP3Milestones for WP3Milestone date

Milestone description Status Partner responsible

Year 1

month 12Material selected for the evaluation activities of the four crop groups (see WP1)

Done (P12 on going)

Pall

Year 2

month 18 Evaluations started

Not yet due P0, P1, P2, P4, P5, P7, P8, P9, P10, P12, P13

Year 3

month 36 70 accessions of lettuce evaluatedNot yet due P1, P4, P7,

P9, P10, P13

month 36 210 accessions of spinach evaluated Not yet due P0, P5

month 36 170 accessions of chicory evaluated Not yet due P2, P8, P12

month 36 30 accessions of minor crops evaluatedNot yet due P3, P5, P9,

P12

Year 4 See deliverables Not yet due


1.1. What is done by who?What is done by who?a) fora) for pests and diseases resistance pests and diseases resistance

Lettuce: Lettuce: 52 evaluations52 evaluations

– Bremia lactucaeBremia lactucae P4 (CZ), P10 (GBR)P4 (CZ), P10 (GBR)

– Root-knot nematodes (Root-knot nematodes (Meloidogyne spMeloidogyne sp.).) P13 (SLO)P13 (SLO)

Spinach: Spinach: 250 evaluations250 evaluations

– Downy mildew (Peronospora farinosa)Downy mildew (Peronospora farinosa) P0 (NLD)P0 (NLD)

Chicory: Chicory: 165 evaluations165 evaluations

– Thielaviopsis basicolaThielaviopsis basicola P2 P2 (FRA) (FRA)

– Alternaria cichoriiAlternaria cichorii P2 (FRA) P2 (FRA)

– Sclerotinia sclerotiorumSclerotinia sclerotiorum P2 (FRA) P2 (FRA)


1.1. What is done by who?What is done by who?b) b) for quality and abiotic charactersfor quality and abiotic characters

Lettuce: Lettuce: 55 evaluations55 evaluations– Post harvest discolorationPost harvest discoloration P1(GBR)P1(GBR)– Winter hardinessWinter hardiness P7 (AUT)P7 (AUT)– Quality, taste (organoleptic)Quality, taste (organoleptic) P7(AUT) ?,P7(AUT) ?, P9 (SLO) P9 (SLO)

Spinach: Spinach: 70 evaluations70 evaluations– Nitrate, oxalic acid, dry matterNitrate, oxalic acid, dry matter P5 (DAN, SW)P5 (DAN, SW)

Chicory: Chicory: 225 evaluations225 evaluations– Shape, color, taste, history, regionalismShape, color, taste, history, regionalismP8 (CHE)P8 (CHE)– SesquiterpenesSesquiterpenes P12 (ITA)P12 (ITA)– Internal sensory characteristicsInternal sensory characteristics P12 (ITA)P12 (ITA)

Valerianella: Valerianella: 9 evaluations9 evaluations– Winter hardiness, tasteWinter hardiness, taste P9 (SLO)P9 (SLO)

Rucola: Rucola: 40 evaluations40 evaluations– Nitrate, dry matter Nitrate, dry matter P5 (DAN, SW)P5 (DAN, SW)– Glucosinolate contentGlucosinolate content P12 (ITA)P12 (ITA)


Evaluation for P0 (CGN)Evaluation for P0 (CGN)

What has been done: What has been done: Start made of Start made of discussing the evaluation of spinach discussing the evaluation of spinach accessions with the breeding accessions with the breeding companies involved.companies involved.

What will be done: What will be done: Finalize the Finalize the plans for the evaluation of spinach plans for the evaluation of spinach accessions.accessions.


Evaluation for P1 : started (WHRI)Evaluation for P1 : started (WHRI)

What has been done: What has been done: The selected gene bank accessions plus The selected gene bank accessions plus

controlscontrols were were assessed for post harvest assessed for post harvest discolouration following established discolouration following established experimental protocols. experimental protocols.

Significant differences were found Significant differences were found between the accessions for mean between the accessions for mean discolouration. The data is still being discolouration. The data is still being analysed as subsets for each day post analysed as subsets for each day post harvest but so far significant differences harvest but so far significant differences have been found between accessions for have been found between accessions for each day analysed. each day analysed.


Evaluation for P2 Evaluation for P2 (GEVES)(GEVES)

What has been done: What has been done: it was considered it was considered as done for P2 and the material was sent as done for P2 and the material was sent to P8. P12 will ask fresh material from to P8. P12 will ask fresh material from P8 and if necessary, P12 could ask seeds P8 and if necessary, P12 could ask seeds to P2 and P3 when he starts evaluation. to P2 and P3 when he starts evaluation.

What will be done: What will be done: Finalize and Finalize and harmonize with partners involved the harmonize with partners involved the scale of notation for evaluation. scale of notation for evaluation. Coordinate the finalizing of evaluation Coordinate the finalizing of evaluation plans for all the crops with the other plans for all the crops with the other leaders of species (P0, P1, P3).leaders of species (P0, P1, P3).


Evaluation for P4 Evaluation for P4 ((Palacky)Palacky)

What has been done: What has been done: Selection of Selection of 30 lettuce accessions for 30 lettuce accessions for characterization, characterization,

In June: Control of viability and In June: Control of viability and pathogenicity of races of pathogenicity of races of Bremis Bremis lactucae, lactucae, pre-selection of isolates to pre-selection of isolates to be used for evaluation in a year 2.be used for evaluation in a year 2.

What will be done: What will be done: Evaluation starts Evaluation starts in year 2.in year 2.


Evaluation for P7 (Evaluation for P7 (Arche Noah)Arche Noah)

What has been done: What has been done: First selection First selection of accessions to be tested for winter of accessions to be tested for winter hardiness. hardiness.

What will be done: What will be done: Developing Developing criteria for evaluate winter criteria for evaluate winter hardiness. hardiness.


Evaluation for P9 Evaluation for P9 (USOFA)(USOFA)

What has been done: What has been done: Evaluation for Evaluation for important property and quality for important property and quality for us.us.


Evaluation for P10 Evaluation for P10 ((HDRAHDRA))

What has been done: What has been done: Material for Material for evaluation (lettuce) already evaluation (lettuce) already selected (all 17 Heritage Seedselected (all 17 Heritage Seed Library accessions).Library accessions).

What will be done: What will be done: Nothing planned Nothing planned until year 2until year 2


Evaluation for P12 Evaluation for P12 ((University of Bologna)University of Bologna)

The aspect of sesquiterpenes is still The aspect of sesquiterpenes is still open. In fact most of what was published open. In fact most of what was published in literature did not remain in practical. in literature did not remain in practical. Some delay will occur in the starting of Some delay will occur in the starting of the real evaluation phase, but this will the real evaluation phase, but this will affect only the time development of the affect only the time development of the program and not the global results. program and not the global results.

Bottlenecks: Bottlenecks: restudy analytical aspect for restudy analytical aspect for sesquiterpenes. sesquiterpenes.


Inquiry about the scale of notation for WP3Inquiry about the scale of notation for WP3

*Which scale of notation ?*Which scale of notation ?

* Which data format will be sent?* Which data format will be sent?1.1. A note with a level of expression associatedA note with a level of expression associated. .

Ex : 1= susceptible ; 9 = resistantEx : 1= susceptible ; 9 = resistant2.2. A measured noteA measured note. Ex : 50 cm. Ex : 50 cm3.3. A fileA file linked to the accession number but not linked to the accession number but not

advised to sort outadvised to sort out4.4. A textA text included in the database as included in the database as “Remarks“Remarks” ” 5.5. A combinationA combination of these 4 possibilities: Indicate of these 4 possibilities: Indicate

which combination you wish. Ex : 5=1+2+4which combination you wish. Ex : 5=1+2+4


Species

Partners

Pests &Diseases

Which scale of notation?

Which data format will be sent

* (1 to 5)

Lettuce

P4 (Palacky Univ.)

Ales

Bremia lactucae

For the assessment of sporulation intensity on lettuce cotyledons, a scale 0-3 will be used:

0 = no visible conidiophores on leaves;

1 = limited sporulation, sporadic conidiophores present;

2 = <50% of cotyledon area covered with conidiophores;

3 = >50% of cotyledon area covered with conidiophores.

5 = 1+2+3+4The data will be presented in:1) Binary form (+=susceptible,-=resistant);2) Quantitatively (the final value of sporulation

intensity (disease intensity) will be expressed as the percentage of the maximum score);

3) Table will be prepared with these data, i.e. 30 accessions screened by 20 different isolates).

P10 (HDRA)

Margi

Bremia lactucae

1=100% infection 9= 0% infection 5 = 1 + 4

P13 (KIS)Vladimir

Nematodes R = resistant (GI < 2, Pf < 1)HR = Hypersensitive reaction (GI > 2,

Pf < 1)S = Susceptible (GI > 2, Pf > 1)

1

Spinach

P0 (CGN)Liesbeth

Downy mildew,

3-5 races

Chicory

P2- SNESValérie

Thielaviopsis basicola

R = resistant ; S = Susceptible According statistics: Necrotic Root

Index:0= no symptoms to 4=100% necrotics

5 = 1 + 4

P2- GEVESValérie

Alternaria cichorii

R = resistant ; S = Susceptible According statistics: 1=0% infection to 9= 100% infection

5 = 1 + 4

P2-FNPEValérie

Sclerotinia sclerotiorum

R = resistant ; S = Susceptible According statistics: classes of

necrosis:0= no symptoms to 5= Chicon wilt

5 = 1 + 4

Scale for pests and diseases : harmonization on goingScale for pests and diseases : harmonization on going


Scale for quality and abiotic characters : a few answers 1/2Scale for quality and abiotic characters : a few answers 1/2Species Partners Evaluation Which scale of notation? Which

data format

Lettuce P1 (HRI)Dave

Post harvest discoloration

P7 (Arche Noah)Franziska

Winter hardiness

1) 1=no winterhardiness ; 9=complete winter hard2) % for how much plants survived 3) cm for head measure4) text for remarks

1+2+4


Taste organoleptic

Same scale of notation than P9


Quality Same scale of notation than P9 1

P9 (USOFA) and P13 (KIS)Mihaela and Vladimir

Taste organoleptic

P9 (USOFA) and P13 (KIS)Mihaela and Vladimir

Quality Type of lettuce (1 = butterhead, 2 = crisphead-batavia, 3 = crisphead-iceberg, 4 = cutting (leaf), 5 = cos (Roman))Earliness - time of harvest maturity, evaluation 1 to 9 (3 = early, 5 = medium, 7 = late)Beginning of bolting, evaluation 1 to 9 (3 = late, 5 = medium, 7 = early)Plant height in cm (average of 10 plants)Plant width in cm (average of 10 plants)Plant weight in g (average of 10 plants)Plant colour (1 = yellow, 2 = yellow green, 3 = green, 4 = red)Colour intensity, evaluation 1 to 9 (3 = light, 5 = medium, 7 = dark)Anthocyanin coloration (1[XXX1] = present, 2 = absent)Head density, evaluation 1 to 9 (3 = dense, 5 = medium, 7 = loose)Leaf blistering, evaluation 1 to 9 (1 = absent or very weak, 5 = medium, 9 = very strong)Marketable part weight in g (average of 10 plants)Marketable yield (t/ha) (average of 3 repetitions of cca 20 plants)Total yield (t/ha) (average of 3 repetitions of cca 20 plants)

1


Scale for quality and abiotic characters : a few answers 2/2Scale for quality and abiotic characters : a few answers 2/2

Species Partners Evaluation Which scale of notation? Which data

format?

Spinach P5 (NGB)Katarina

Nitrate

P5 (NGB)Katarina

Oxalic acid

P5 (NGB)Katarina

Dry matter

Chicory P8 (Pro Specie Rara) Bela

Shape, color, taste, history, regionalism

3 ?

P12 (UNIBO)Filippo

Sesquiterpene lactonesexternal

2

P12 (UNIBO)Filippo

Internal sensory characteristics

1

Valerianella P9 (USOFA)Mihaela

Winter hardiness

P9 (USOFA)Mihaela

Taste (organoleptic)

Rucola P5 (NGB)Katarina

Nitrate

P5 (NGB)Katarina

Dry matter

P12 (UNIBO)Filippo

Glucosinolate content

1


What about dissemination of What about dissemination of results?results?

Documents

V Cadot,: WP meeting Leafy Vegetables 27th September 2007 WP meeting Leafy Vegetables WP 3 Cadot Valérie GEVES : Variety and seed, study and control group