yaghoob.alvar@ut.ac.ir

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The Effect of Eight Weeks of High-Intensity Interval Training on Gene Expression of

Angiogenesis Factors in Ischemic Rats

Mehri Alvar Y1, Gaeeni AA

2, Ramezani AR

1, Golab F

3, Gheiratmand R

4, Barati M

5

1Dept. of Exercise Physiology, Shahid Rajaee Teacher Training University, Tehran, Iran

2Dept. of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran 3Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran

4Dept. of Cardiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran 5Dept. of Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran

Corresponding Author: Mehri Alvar Y, Dept. of Physical Education and Sport Sciences, Shahid Rajaee Teacher

Training University, Tehran, Iran

E-mail: yaghoob.alvar@ut.ac.ir

Received: 16 Jul 2017 Accepted: 19 Sep 2017

Background and Objective: Myocardial infraction can lead to loss of heart muscle cell, changes in

ventricular function and structure, scar formation as well as leading the heart to ultimate stroke and its loss.

This study aimed to investigate the effect of eight weeks of High-Intensity Interval Training (HIIT) on the

gene expression of angiogenesis factors in ischemic rats.

Materials and Methods: In this study, 28 male Wistar rats (200-250 g) were randomly assigned to 4 groups:

sham, ischemia, exercise, and exercise-ischemia. Myocardial infarction (MI) was achieved via closure of

LAD artery for 30 minutes. A treadmill exercise program was performed by the rats for 8 weeks, 40 minutes

daily, 3 days a week.

Results: The results showed that there is a significant difference between the four groups in gene expression

levels of VEGF, apelin and apelin receptor (p = 0.001). Results also showed that the gene expression level of

VEGF, apelin and apelin receptor was significantly increased in the exercise-ischemia group compared to

sham (p = 0.04), ischemia (p = 0.002) and exercise (p = 0.009) groups. Results showed that exercise during

ischemia can lead to an increase in VEGF gene expression but not in exercise group alone. On the other

hand, a significant increase was observed in gene expression level of apelin following ischemic intervention

compared to the sham group (p = 0.025). No difference was observed between the interval training group and

sham group (p > 0.05). Meanwhile, a significant reduction in the gene expression level of apelin receptor

was seen following ischemic intervention compared to the sham group (p = 0.04).

Conclusion: The physiological adaptations caused by interval training are associated with different gene

expressions. A regular interval training program leads to the increase of factors involved in angiogenesis and

the regeneration of cardiac muscle vessels following myocardial ischemia.

Keywords: Myocardial ischemic, Angiogenesis, Intensity interval training, Apelin