I. K. Robinson, Ptycho 2013 1

Use of Ptychography to detect Phase Shifts in Crystals during Diffraction

Ian Robinson Jesse Clark Ross Harder Maria Civita Laura Shemilt Joerg Schwenke Yusuf Mohammed Ivan Vartaniants

London Centre for Nanotechnology Research Complex at Harwell

Ptycho2013,

Schloss Hohenkammer, Germany, May 2013

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Outline •  Ptychography measures phase •  Crystallographic phase •  Experiment on Au nanocrystals •  Chromosome imaging

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X-ray Ptychography J. Rodenburg et al, PRL 98, 034801 (2007)

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Reconstruction of Probe P. Thibault et al, Science 321 379 (2008)

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Emil Wolf’s Phasing Scheme

Physics Letters A 374 491 (2010)

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Ptychography of Gold Nanocrystals Maria Civita, UCL, Ana Diaz, Swiss Light Source

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Swiss Light Source cSAXS beamline

8 I. K. Robinson, Annecy 2012

9 I. K. Robinson, Annecy 2012

Ptychography of Gold Nanocrystals Maria Civita, UCL, Ana Diaz, Swiss Light Source

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Transmitted Phase of Gold Nanocrystals Maria Civita, UCL, Ana Diaz, Swiss Light Source

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Diffraction from a Finite Slab Als-Nielsen and McMorrow, “Modern X-ray Physics”

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Diffraction from a Finite Slab Als-Nielsen and McMorrow, “Modern X-ray Physics”

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R

T=1-R

Ptychography of Gold Nanocrystals Maria Civita, UCL, Ana Diaz, Swiss Light Source

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Dynamical Theory (Ivan Vartaniants)

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t=100nm t=110nm

Ptychography at 34-ID-C (APS) nPoint 3D scanner, Ross Harder and David Vine

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Ptychography at 34-ID-C (APS) nPoint 3D scanner, Ross Harder and David Vine

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#S159  Isolated  human  chromosome  on  SiN-­‐membrane  Reconstructed  from  ptychography  scan  with  transparent  beamstop.  

Phase  shift  (rad)  

Transmission  (100%)  

Chromosome Ptychography Laura Shemilt, Joerg Schwenke, Yusuf Mohammed, 34-ID-C

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#S152, frame 1 10s exposure 6.5 keV Medipix2, 55 µm 1.851 m distance

75 nm

50 nm 50 nm

Inte

nsity

(log

10)

50 nm

Chromosome Ptychography Laura Shemilt, Joerg Schwenke, Yusuf Mohammed, 34-ID-C

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Conclusions and Acknowledgements •  Crystallographic phase determination •  Absolute phase of Structure Factor •  Ripples in images due to beam stop?

Ana Diaz Swiss Light Source Cameron Kewish Manuel Guizar-Sicairos Ross Harder Advanced Photon Source