Urinary Tract System

• Early morning midstream urine sample.

• Cultured on Macconkey and blood agar .

• Small loop 1 µl count colonies* 1000(0.01 ml)• Large loop 10µl count colonies* 100(0.1ml)• 10 ^5 > significant infection.• 10^3-10^5 intermediate infection.• 10^3 < Not significant.• Colony count unite : CFU (colony forming unite).

(E.coli) 1. Escherichia coli

2. Klebsiella

o Gram negative rods , nonlactose fermenter

o Swarming motility ( flagellated )

o Prevent swarming by culturingit on CLED or Macconkey media

Proteus

Biochemical tests for E.coli

1) kligar sugar iron:

• For E.coli :

Acid/acid (A/A)(Y/Y)

Gas +

No H2S

Kligler test

Biochemical tests for E.coli

2) Citrate test :

Negative for E.coli

Biochemical tests for E.coli

3) Urease test :

Negative for E.coli

Biochemical tests for E.coli

4) SIM (SULFIDE, INDOLE, MOTILITY) MEDIUM

Indole positiveSulfide (H2S) = negativeMotility =motile

Neg Pos

identified by :

•Gram negative rods

•odor : grape-like smell.

•Oxidase positive.

•Produce different pigments colors:• Pyocyanine (blue-green)

• Pyoverdin (fluorescent yellow –greenish pigment).

• Pyorubrin (red).

• Pyomelanin (brown).

Pseudomonas aeruginosa

Pseudomonas aeruginosa on mueller hintion agar

Blood agar

A- staphylococcus .albus

B- staphylococcus.Aureus

CLED media Lactose fermentation ( +ve )

staphylococcus spp.

Test for differentiation of Staphylococcus species

Catalase test

MSAMannitol salt agar media

S.aureus

S.albus

Bile Esculin Test

Neiserria

Intra cellular

Extra cellular

Gram

Negative

diplococci

Neisseria

IntracellularExtracellular

Haemophilius ducreyi (cancroid)

Schistosoma haematobium worms

Schistosoma haematobium egg

Trichomonas vaginalis parasite

Treponema pallidum

Candida Species

Candida

Spp.

C.albicans

C.tropicalis

C.glabrata

C.Krusei

Candida Spp

• Larger than Bacteria

• Budding

sabouraud dextrose agar

To Differentiate between C.albican and other Species

Germ tube[ Serum + candida ]

2. Chrom agar

C.glabrata : violet (dark pink)

glistering C.albicans : Green

C.TropicalisBlue

C.Krusei : rough dry pale pink

Chrom agar