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Updates in Flow Cytometry
Date
21st– 22nd July 2016
Introduction
In the last two decades, flow cytometricindispensable part of the armamentarium for diagnosis, prognostication and monitoring of haematolymphoid malignancies. More sophisticated instruments, newer fluorochromes and ever expanding list of clinically useful antibodies has led to delineation of disease profiles and deeper appreciation of normal haematopoiesis. dependent. In Europe, there have been effortsprocess from instrument set-up, compensation to sample preparation to antibody/fluorochrome combinations, in order to optimize the precision and interThis is the ongoing work of the Euroflow Consortium, which has culminated istandardization between labs. This meeting will present how this has changed the landscape of flow cytometry and provide further insights into the next era of clinical flow cytometry.
Objectives
Appreciate normal immunophenotypes of appreciate how disease states deviate from the normal maturation states
Discussion of minimal residual disease monitoring clinical analysis
Introduction to the work and objective Networking and interactive exchange of ideas and experiences between participants and
speakers
Target Participants
Anyone interested in improving the practice of flow cytometry in their lab Pathologists with interest in Haematologists Technical officers involved in flow cytometry Researchers
in Flow Cytometry for Haematolymphoid Malignancies
flow cytometricimmunophenotyping has been established as an indispensable part of the armamentarium for diagnosis, prognostication and monitoring of haematolymphoid malignancies. More sophisticated instruments, newer fluorochromes and ever
eful antibodies has led to delineation of disease profiles and deeper appreciation of normal haematopoiesis. Analysis is increasingly complex and even expert
have been efforts to systematically study and refine the whole up, compensation to sample preparation to antibody/fluorochrome
combinations, in order to optimize the precision and inter-laboratory comparability of results. This is the ongoing work of the Euroflow Consortium, which has culminated in the adoption of
This meeting will present how this has changed the landscape of flow cytometry and provide further insights into the next era of clinical flow cytometry.
Appreciate normal immunophenotypes of haematopoietic and lymphoid maturatappreciate how disease states deviate from the normal maturation states
al residual disease monitoring – achieving technical
Introduction to the work and objectives of the Euroflow approach nteractive exchange of ideas and experiences between participants and
in improving the practice of flow cytometry in their labPathologists with interest in haematolymphoid malignancies
Technical officers involved in flow cytometry
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for Haematolymphoid Malignancies
been established as an indispensable part of the armamentarium for diagnosis, prognostication and monitoring of haematolymphoid malignancies. More sophisticated instruments, newer fluorochromes and ever
eful antibodies has led to delineation of disease profiles and deeper Analysis is increasingly complex and even expert-
atically study and refine the whole up, compensation to sample preparation to antibody/fluorochrome
laboratory comparability of results. n the adoption of
This meeting will present how this has changed the landscape of flow cytometry and provide further insights into the next era of clinical flow cytometry.
etic and lymphoid maturation, and
achieving technical results and
nteractive exchange of ideas and experiences between participants and
in improving the practice of flow cytometry in their lab
Speakers/Panel
1) Professor Alberto Orfao Professor of Medicine and Director of the General Cytometry Service and
Principal investigator of the Center for University of Salamanca,
2) Dr. Te Chih Liu Head, Haematology Division, Department of Laboratory Medicine,National University Hospital, Singapore
3) Dr. Paul Edward HutchinsonHead, Immunology Program Flow Cytometry Laboratory,Life Sciences Institute, National University of Singapore
4) Dr Shao-Min Han Consultant Haematologist,Veteran General Hospital
Submission of Cases
Participants are invited to submit2016 to Dr Lee Shir Ying at [email protected]
Quality control and technical optimization questions for
Send the following: i. Instrument in use
ii. Calibration usediii. Staining and lysing protocoliv. Technical question
Diagnostically challenging cases for Case Discussion Session
i. Clinical history and diagnostic questionii. Screen shots of analyzed data highlighting the population of interestiii. Results of other testing such as histology, imaging, cytogene
molecular if availableiv. Email FCS file (Zip format) by
Venue
Dental Auditorium, Level 4, DentalNationalUniversity Hospital. 5 Lower Kent Ridge Road, Singapore 119074See map attached for direction
Professor of Medicine and Director of the General Cytometry Service andPrincipal investigator of the Center for Cancer Research (CIC-IBMCC)University of Salamanca, Spain
Head, Haematology Division, Department of Laboratory Medicine,National University Hospital, Singapore
Paul Edward Hutchinson Head, Immunology Program Flow Cytometry Laboratory, Life Sciences Institute, National University of Singapore
Consultant Haematologist, Veteran General Hospital
Participants are invited to submit cases for case discussion with the panel. Email by 312016 to Dr Lee Shir Ying at [email protected]
Quality control and technical optimization questions for Case Discussion Session 1
Instrument in use Calibration used Staining and lysing protocol Technical question
Diagnostically challenging cases for Case Discussion Session 2. Send the following:Clinical history and diagnostic question Screen shots of analyzed data highlighting the population of interestResults of other testing such as histology, imaging, cytogenetics and molecular if available Email FCS file (Zip format) by 31 May 2016 to Dr Lee Shir Ying [email protected]
Dental Centre
5 Lower Kent Ridge Road, Singapore 119074
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Professor of Medicine and Director of the General Cytometry Service andIBMCC)
Head, Haematology Division, Department of Laboratory Medicine,
cases for case discussion with the panel. Email by 31st May
Case Discussion Session 1.
. Send the following:
Screen shots of analyzed data highlighting the population of interest tics and
to Dr Lee Shir Ying
Program
Day 1: 21st July 2016
Time Agenda
8:00am – 8:30am Registration and Breakfast
8:30am – 8:45pm Welcome addressDr. TeChi
8:45am – 9:45am Keynote LectureProf Alberto Orfao, University of Salamanca, Spain
9:45am – 10:15am Forum: What is the value of flow cytometry in haematolymphoid malignancies in 2016?Discussants: ProfModerator
10:15am – 10:30am Tea break
10:30am – 11:45am Keynote Lecturelymphoid maturationProf Alberto Orfao, University of Salamanca, Spain
11:45am – 1:00pm Case discussion 1: Quality control and technical optimization Panel: Prof Alberto Orfao, University of Salamanca, SpainDr. TeChih
1:00pm – 1:15pm Question and Answer
1:15pm – 2:00pm Lunch
2:00pm – 3:15pm Case discussion Panel: Prof Alberto Orfao, University of Salamanca, SpainDr.TeChih
3:15pm –4:30pm Keynote clinical aProf. Alberto Orfao, University of Salamanca, Spain
4:30pm – 4:45pm Tea break
4:45pm –5:30pm Real life userDr. Shao
5:30pm – 5:45pm Question and Answer
End of Day 1
Agenda
Registration and Breakfast
Welcome address Chih Liu, National University Hospital, Singapore
Keynote Lecture 1: Overview of the Euroflow approachProf Alberto Orfao, University of Salamanca, Spain
Forum: What is the value of flow cytometry in haematolymphoid malignancies in 2016? Discussants: Prof. Alberto Orfao, participants Moderator: Dr. Shir Ying Lee, National University Hospital, SingaporeTea break
Keynote Lecture 2: Overview of normal haematopoiesis and lymphoid maturation Prof Alberto Orfao, University of Salamanca, Spain
Case discussion 1: Quality control and technical optimization Panel: Prof Alberto Orfao, University of Salamanca, Spain
TeChih Liu, National University Hospital, Singapore
Question and Answer
discussion 2: Diagnostic challenges Panel: Prof Alberto Orfao, University of Salamanca, Spain
TeChih Liu, National University Hospital, Singapore
Keynote Lecture 3: Minimal residual disease –Technical and aspects
Alberto Orfao, University of Salamanca, Spain
Tea break
Real life user’s experience in the Euroflow method Shao-Min Han, Taiwan
Question and Answer
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1: Overview of the Euroflow approach
Forum: What is the value of flow cytometry in haematolymphoid
g Lee, National University Hospital, Singapore
2: Overview of normal haematopoiesis and
Case discussion 1: Quality control and technical optimization
Technical and
Day 2: 22nd July 2016
Time Agenda
8:00am – 8:30am Breakfast
8:30am – 9:30am Keynote Lecture4directionsProf. Alberto Orfao, University of Salamanca, Spain
9:30am – 10:15am Can we have a clinical flfor the South East Asian region?Dr. Paul Hutchinson
10:15am – 10:45am Forum: How to adopt the Euroflowapproach in an economically lean hospitalDiscussants: Prof Alberto Orfao, participantsModerator
10:45am – 11:00am Tea break
11:00am – 12:15pm Case discussion Panel: Prof Alberto Orfao, University of Salamanca, SpainDr. TeChih
12:15pm – 12:45pm “One FlowDr. Paresh, Becton Dickinson
12:45m – 1:15pm Question and Answer
1:15pm – 1:30pm SummationDr. TeChih
1:30pm – 2:30pm Farewell
End of Day 2
Registration Information
Please send your completed registration formfax to +65 6774 5203 before June 30, 2016
For enquiry, please contact [email protected]
Agenda
Breakfast
Keynote Lecture4: Current status of flow cytometry and future directions
Alberto Orfao, University of Salamanca, Spain
Can we have a clinical flow cytometry quality assurance program the South East Asian region? Paul Hutchinson, Life Sciences Institute, NUS, Singapore
Forum: How to adopt the Euroflowapproach in an economically lean hospital Discussants: Prof Alberto Orfao, participants Moderator: Dr. TeChih Liu, National University Hospital, SingaporeTea break
discussion 3: Minimal residual disease Panel: Prof Alberto Orfao, University of Salamanca, Spain
TeChih Liu, National University Hospital, Singapore
One Flow” solution to instrument setup and QC esh, Becton Dickinson
Question and Answer
Summation TeChih Liu, National University Hospital, Singapore
Farewell Lunch
Please send your completed registration form (page 5) to [email protected] June 30, 2016.
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Current status of flow cytometry and future
ssurance program
, Life Sciences Institute, NUS, Singapore
Forum: How to adopt the Euroflowapproach in an economically
, Singapore
Registration Form (for individual or group registration)
S/N
Participant Name
Salutation (Prof/ Dr/ Mr/ Ms/ Mdm)
Last Name First Name
1
2
3
4
5
(for individual or group registration)
Designation Department &
Institution Name Email
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Email Contact Number:
