Unlock biomolecUle qUanTificaTion in UV/ViS

wiTh DropSenSe 96® DropleT SpecTral conTenT profiling

The Trinean DropSense 96® is an innovative spectro photometer generating high quality spectral data

➜ Instant full UV/VIS spectral analysis (220-750 nm)➜ Flexible read-out of DropPlates or standard 96 well plates➜ Label-free DNA/RNA and protein quantification➜ 0,1 - 200 OD (10 mm equivalent, DropPlate D+)➜ < 5 min for 96 samples➜ Parallel optical channel for lamp monitoring➜ Operated with DropQuant software➜ Integration into liquid handlers

DropSenSe 96®

polyChromatiC droplet plate reader

Xenon lamp

DropPlate

Lens Mirror

Grating Detector

This proprietary, compact multichannel spec-trophotometer uses a central cylindrical lens for assembly of 4 separate optical channels in parallel, leading to highly reproducible and high quality data. A full spectral analysis is performed within a single lamp flash (50ms).

In the picture, one optical channel is represented.

dropsense 96® optiCal desiGn

DropSenSe 96®

polyChromatiC droplet plate readerDropqUanT

intUitive experiment desiGn and measUrinG software

The DropQuant software is easy to operate with extensive options for experimental set-up, plate layout and import/export flexibility

➜ Quick experiment definition and import (xls, csv, txt)➜ Full UV/VIS spectral display➜ A260 and A280 data for DNA/RNA/protein quantification➜ Direct link to cDrop software for in-depth composition analysis➜ Easy export or customized report options (xls, pdf, csv, txt)➜ LIMS compatible

Add-on functionalities:

➜ 21 CFR Part 11 compliant software version➜ Integrated IQ/OQ verification➜ DropControl software (API) for robot integration

➜ DropQuant softwareQuantification of DNA and RNA based on A260 nm absorbance (or A280 nm for pro-teins) for conventional concentration deter-mination. Absorbance ratios (A260/A280, A260/A230) are available.

➜ cDrop™ softwareSpecific quantification of biomolecules (DNA, RNA, proteins, oligo’s & PCR products) in complex samples based on proprietary spec-tral content profiling method. Profiling the unique UV/VIS spectral shape of a sample al-lows both specific quantification and content QC by impurity identification.

the trinean ways for dna, rna or protein qUantifiCation

cDrop™ Sample conTenT analySiS

speCtral Content profilinG for trUe qUantifiCation in Uv/visThe Trinean cDrop™ software is a novel tool for specific dye-free quantification of biomolecules in complex samples. The UV/VIS spectral meas-urements by the DropSense 96® are analyzed by the cDrop™ software to extract accurate quanti-tative information by defining the spectral profile of the isolates of interest and contaminants like proteins, salts and phenol in the sample.

cDrop™ features➜ Display of the measured UV/VIS spectra➜ Selection of cDrop protocol based

on extraction or assay protocol➜ Spectral content profiling for specific

quantification➜ Additional contamination

and turbidity analysis➜ Plate overview of cDrop results

for overall extraction QC

cDrop™ key domains➜ Accurate normalization before PCR/qPCR➜ DNA or RNA isolation optimization➜ Dye-free protein quantification➜ Biostorage QC➜ Sample QC before (NGS) sequencing➜ Quick PCR amplification analysis

cdrop™ introdUCtion

cdrop™ how does it work

A260 Conc (ng/ul)

A260/A230 A260/A280

116.89 2.35 1.88181.65 2.39 1.89139.32 2.34 1.8

➜

Measure UV/VIS spectra + A260 or A280 quantification

2 µl DNA samples on a DropPlate are measured by the DropSense96. Full spectral curves and concen-trations (A260) are shown in the user software. Varying concentra-tions are found while additional ratio's (260/230 + 260/280) indicate that all samples contain pure DNA.

+ cDrop

cDrop spectral profiling shows similar DNA amounts in all three samples. Severe contamination by RNA or phenol is found explaining the overestimation by the A260 method and illustrating the insensitivity of traditional A260/A280 ratio’s as purity check.

cDrop™ Sample conTenT analySiS

speCtral Content profilinG for trUe qUantifiCation in Uv/vis

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3 PCR quantification

Example: Whole Genome AmplificationCrude PCR amplicons can be quantified with cDrop™

prior to clean-up by discrimination of the dsDNA amplicon from the abundant presence of dNTPs.

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A280 BCA ASSAy cDROP™ protein

4 Protein quantification

Example: Protein quantification in cell lysatesTotal protein quantification in mixed samples by cDrop™ avoids time consuming dye-based quantification by discrimination of proteins, nucleic acids and buffer components within the measured UV/VIS spectrum.

2 DNA quantification

Example: DNA extract QCComparative analysis of the cDrop™ method with two traditional DNA quantification methods using DNA extracts from large volume blood samples.

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1 RNA quantification

Example: Gene expression RNA QC

Sample prep quantification and QC

Sample QC is a bottleneck in life science applica-tions. Current quantification methods have limita-tions: absorbance is non-specific, leading to over-estimation of impure samples, while dye-based methods are more specific but time consuming and give no quality indication.

cdrop™ appliCations

RNA extracts

High quality RNAOutliers

Specific quantification + Sample QC

RT step + Targetamplification

PCR quantification

Genetic test

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The cDrop™ content analysis of two RNA isolates from Hek 293 cells shows a large difference in thiocyanate salt contamina-tion. RIN scores are shown as an insert.

Key features

➜ 2 µl sample input: minimal sample consumption➜ 16 or 96-well DropPlate formats: fit for low to high throughput➜ Microplate dimensions: automation friendly➜ Bar-coded DropPlate 96: ideal for sample tracking➜ Capillary storage channel: easy loading, protection from

evaporation or cross-contamination➜ Compatible with viscous or concentrated samples:

fit for all kinds of biomolecules

DropplaTeS S anD D+

top-level desiGn miCroflUidiCs to fit hiGh standards

DRoPPlATES 16S

DRoPPlATES 96S

DRoPPlATES 16D+

DRoPPlATES

96D+

NBR of wEllS 16 96 16 96

DRoPfRAME REQUIRED ✔ ✔

BAR-CoDED ✔ ✔

oPTICAl PATh lENghTS 0,5 mm 0,1 mm and 0,7 mm

READINg TIME 96 SAMPlES DropPlate 96S < 5 minutes DropPlate 96D+ < 8 minutes

SAMPlE VolUME 2 µl 2 µl

MEASUREMENT RANgE (oD) 0,1 - 40 OD (10 mm path converted) 0,1 - 200 OD (10 mm path converted)

➜ PRESICIoN Sample < 2 OD: ± 0,03 OD Sample < 2 OD: ± 0,03 OD

Sample > 2 OD: ± 1,5% CV Sample > 2 OD: ± 1,5% CV

➜ ACCURACy Sample < 2 OD: ± 0,04 OD Sample < 2 OD: ± 0,04 OD

Sample > 2 OD: ± 3% CV Sample > 2 OD: ± 3% CV

ExAMPlE: dsDNA 5 - 2000 ng/µl 5 - 10 000 ng/µl

cDRoP™ CoMPATIBlE yes yes

ShElf lIfE 1 year 1 year

DropPlate S

Microfluidic plate with a single measure-ment chamber, ideal for routine genomic applications (DNA and RNA extractions or PCR amplicon quantification).

DropPlate D+

Microfluidic plate with a dual measurement chamber for an extended OD range for broad range applications (from sensitive NGS sample quantification to highly con-centrated proteins or oligonucleotides QC).

dropplates desCription

SAMPlE SIzE 2 ul (DropPlate S and D+)

SAMPlE NUMBER 1 to 96 (flexible use of DropPlate -16 or -96)

PATh lENgTh➜ 0,5 mm on DropPlate S➜ 0,1 mm and 0,7 mm on DropPlate D+

lIghT SoURCE Xenon flash lamp

oPTICAl SySTEM 5-channel polychromator: 4 parallel sample channels + 1 reference channel

wAVElENgTh RANgE 230 - 750 nm

wAVElENgTh RESolUTIoN 3 nm

wAVElENgTh ACCURACy 0,5 nm

wAVElENgTh REPRoDUCIBIlITy 0,1 nm

ABSoRBANCE RANgE

➜ 0,1 to 40 OD on DropPlate S (10 mm equivalent)➜ 0,1 to 200 OD on DropPlate D+ (10 mm equivalent)

MEASUREMENT TIME

< 4 seconds per sample on DropPlate S

DIMENSIoNS W: 34 cm x L: 45cm x H: 35 cm

oPERATINg VolTAgE 24 VDC

CoNNECTIoN USB

APPRoVAl CE

Measurement cycle in DropPlate D+

General speCifiCations

1 Step 1

Sample is dispensed into the input well ei-ther manually or by a liquid dispensing robot.

2 Step 2

Sample is drawn into the reservoir by capil-lary action. The DropPlate can now be load-ed on the DropSense 96®. A measurement of the empty microcuvette is performed to determine the absorbance of the plastic.

3 Step 3

The first micro-cuvette is filled by applying a small vacuum pressure in the channels. A second absorption measurement is per-formed using a small path length for highly concentrated samples.

4 Step 4

The second micro-cuvette is filled by con-tinued vacuum pressure. A third absorption measurement is performed with a large path length for low concentrated samples.

Trinean nvDulle grietlaan 17/3, B-9050 Gentbrugge, Belgium +32 (0) 9 272 75 35, info @ trinean.com, www.trinean.com

Trinean nV

a spin-off from Ghent University and imeCinnovative solUtions for miCro-sample qUantifiCation & qC

➜ Fits 96-well sample handling➜ Stand-alone or robot integration➜ Micro-sample loading & preservation➜ Bio-sample QC by spectral content profiling➜ CFR21 Part 11 compliance

trinean prodUCts

➜ Fits single tube sample handling➜ Compact, all-in-one design➜ Micro-sample loading & preservation➜ Bio-sample QC by spectral content profiling➜ 'Touch & Go’ use (~1 minute for 16 samples)