University of Groningen

Foa-Kurloff cellen. De invloed van enige hormonen, in het bijzonder geslachtshormonen, ophet aantal Foa-Kurloff cellen in het bloed van de caviaLouwerens, Bernardus

IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite fromit. Please check the document version below.

Document VersionPublisher's PDF, also known as Version of record

Publication date:1952

Link to publication in University of Groningen/UMCG research database

Citation for published version (APA):Louwerens, B. (1952). Foa-Kurloff cellen. De invloed van enige hormonen, in het bijzondergeslachtshormonen, op het aantal Foa-Kurloff cellen in het bloed van de cavia Groningen: s.n.

CopyrightOther than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of theauthor(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons).

Take-down policyIf you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediatelyand investigate your claim.

Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons thenumber of authors shown on this cover page is limited to 10 maximum.

Download date: 10-02-2018

rum, Íntraveneus ingespo-.4, het aantal granulocy-van een cavia met weinignbl Ík van b loedafnemen,en neiging tot dalen van: hebbenij vaststellen:Itstreekse invloed op het

;ecundaire invloed op hethet ovarium tot vergrote

;, hebben geen invloed.l . F.K.cel len wordt w&&r-de oestradiol concentra-

c blj de mlnlmun dosís.

op over de functle vanrrtbestaan van de F.K.ceIn het onderzoek zou kun-

S U M M A R Y

In the b lood of the guinea p ig a ceI I is found that can mosteasi ly be descr ibed as a b ig lymphocyte wi th one, somet imes twovacuoles. In the bloodsmear, stained according to May Griinwald-GÍemsa, this vacuole appears to be fi l led with fi laments or gra-nules consisting of a substance which is stained in about the sameway as the nucleus. After the investigators who were the first toreport on these cells, they are called Fbà-Kuloff cel1s (abbrevi-a ted to F .K .ce1 l s ) .

A number of hypotheses have been put forward on the origin andnature of these cells. The most probable one is based on the im-por tant fact that the F.K.cel Is increase af ter admlnis t rat ion ofoestradio l and that testosterone is supposed to have a s imi laref fect .

The purpose of our investigatlon was to obtain more informa-t ion about the act ion of sex hormones on the F.K.ce1ls in maleand fenale guinea pigs. Inaddition by removal of endocrine glandsor by administering other hormones we tried to establish if otherendocr ine.organs but sex organs af fect the number of F.K.ceI ls .Since there is no agreement about this we finally tried to esta-b t i sh i f a co r re la t i on ex i s ted be tween the number o f F .K .ce l l sand the nrmber of any other cells of the white blood picture.

ïhe substances were always administered subcutaneously. Begin-nÍng with the day of lnjection the blood ce1ls were counted everytwo days (our schedule was: blood sampling on l, londag \ïednesdayand Friday, or Tuesday, Thursday and Saturday in the afternoon).We counted the total number of the white cells and differentiatedinto lymphocytes, eosinophil-e granulocytes, other granulocytes andF. K. ce l 1s.

Our results, also mentioned at the end of the chapters, were:Chapter IV.1.One iq 'ect ion of oestradio l causesapeak in the number of c ' .K.

cells 8 to 14 days after the administration Whereas in normalguinea pigs about 3 per cent of the white bloodcells appear tobe F.K. ceI ls , th is percentage increases t i11 8 to 10 per centafter the administration of oestradiol. There also occur peaksfrom 16 to 20 per cent, but these were exceptions.. In the maleguinea pig the normal nunber of 1 to 2 per cent F.K. cells in-creases af ter adminis t rat ion of oestradio l t i l l 8 to 10 percent in about ten days.

2. In the normal female the smallest effective dose of oestradioll ies between 1 y and 2.5 y oestradiol per 100 g of bodyweight.

3. Higher dosages do not have a greater effect.

8 ?

4. Bepeated administration of oestradiol does not change the ef-fect mentioned under 1.

5. l.lormal male animals respond to an administration of oestradiolroughly in the same way as female animals, but the effect onthe p'.K.ce1Is is of longer duration: whereas in female animalsafter the highest peak the normal level of the p.K. cells re-turned in about five days, in male animals it renained hj.gh forsone weeks after the peak.

6. In the normal fenale guinea pig testosterone causes a rise inthe number of F.K. cells of the same nature as that caused byoestradiol. The effect, however, is less pronounced.

7. Progesterone appeared to have no effect on the number of F.K.cells in the normal female guinea pig;

Chapter V,8. In the male animal castration has no effect on the number of

F .K . ce11s .9. Castration does not change the effect of oestradiol.10.The number of F.K. ce l ls in the female animal decreases af ter

ovariectomy. We failed to observe the temporary increase of thelevel of the t r .K. ce l ls immediate ly af ter the operat Íon whichis reported by others.

11.oestradio,l raises the number of F.K. cells in the ovariectomizedguinea pig, but the effect is less pronounced as the time be-tween the operation and the injection of oestradiol is longer.

l2.Subcutaneousimplantation of two tablets of 25 mg of oestradiolberzoate in ovariectomized animals caused a peak of the numberof F.K.ce11s, which is s imi lar to the peak af ter one in ject ionof oestradiol in the nornal female. After the peak the levelof the F. !L cel ls remains increased for some t ime. Af ter re-moval of the implant the nunber of F.K. cells decreased. Thisdecrease is not preceded by an increase (compare 10).

12. In dosages up to 0.5 ng per 100 g of bodyweight testosteronehas no ef fect on the number of F.K- cel ls inovar iectomizedguinea p igs.

13. In ovar iectomized animals, which level of the F.K. ce l ls wasmaintained on about 3 per cent of the number of white blood-cells by means of an oestradiol benzoate implant, testosteroneproved to have no effect on the number of F.l(. cells.

Chopter Y) .l5.Neither adreno-corticotropic hormone (AC1Ïl) nor desoxycortico-

steronacetate (DcA) were able to affect the number of clrcul-ating F.I(. cells in the normal female guinea pig.

16.Rènroval of the adrenal glands did not change the numberofF.K.ce1ls in the female guinea pig.

lT.Hypophysectomized females respond to one injection of oestra-

88

dio lder 1

18. Afterthe b

19. Adnln,cel ls

20. Afterhad a

Chapter I21. Togetl

alwayssequela decrmean trlse 1

Clwpter I22.No cor

other23. Intrav

plg wlper cefemalewith frcrease

0n theí .Oes t ra r2. Testos'

F.K.ce,of cs

J. As far4. Tlte ln

probab

F.K. cr5. lhe opt

dose.

FinalIoestradiofew sugge

)es not change the ef-

stration of oestradiol, ls, but the effect onreas in female animalso f t he F .K . ce l l s re -

s it reneined high for

Srone causes a rise inbure as that caused by, pronounced.on the number of F. K.

lfect on the number of

I oestradiol.animal decreases afterrpo{ary increase of theer the operation which

Ls in the ovariectomizedounced as the time be-' oestradiol is longer.of 25 mg of oestradiolI a peak of the numberak after one injection;er the peak the level' sonp time. After re-cel ls decreased. This(compare 10).dWeight testosteronel ls in ovar iectomized

l f t he F .K . ce l l s wasrmber of white blood-implant" testosteroneI F. l ( . ce l ls .

t l) nor desoxycortico-lhe number of clrcul-nea pig.

nge the number of F. K.

injection of oestra-

diol with a rise of the number of F.K. cells, as mentioned un-de r 1 .

18.After hwophysectomy the F.K ce1ls gradually disappear fromthe blood.

19.Admlnistration of adrenaline does not change the numberofF.K.cells ln normal femles.

20.After renoval of the uterus (1n only two anlmals) oestradiolhad a prolonged action.

Chapter VII.2 l .Together wi th the increase of the number of F.K. ce l ls there

always occurs a rise ln the nrnnber of granulocytes and the sub-sequent decrease of the F.K. cells is l lkewlse accompanied bya decrease of the granulocytes. On the contrary thls does notmean that the number of F.lG cells increases when there is arlse ln the granulocytes caused by some other reason

Chopter VIII.22.No correlation ïras found between the number of F.lL cêlls and

other cells of the whlte blood plcture.

23.Intravenous administration of 2 ml serum obtained fromaguineapig wlth a number of F.K. cells in the blood of more than 10per cent, lncreased the number of granulocytes of the normalfenale guinea pig. Onthe contrary serum obtained fromananimalwith few F.I(. cells in the blood caused a tendency towards de-crease of the nunber of granulocytes.

0n the basls of these data it is concluded:í. Oestradlol has a dlrect effect on the number of F.K. cells.2. Testosterone probably has an indirect effect on the number of

F.K.celIs. This honpne is supposed to stlmulate the secretionof oestradlol by the ovary.

J- As far as investigated other hompnes have no effect.4. Ttie lncrease of the oestradlol concentration of the blood is

probably the stimulus whlch caused the rise of the number ofF.K. ce l ls .

5. the optimal dose of oestradiol is nearly the same as the minimaldose.

Finally a hypothesis was put forward about the importance ofoestradiol to the origin and maintenance of the F.K. cells and afew suggestions were given for continuing the investigations.

89