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7/30/2019 Umn June2011 Eijsink Web
1/8
NORWE
GIAN
UNIV
ERSITY
OFLIFE
SCIEN
C
ES
www.umb.no
Departm
en
tof
Ch
e
mistry,Bio
techn
olo
gyan
dFoodScien
ce
Team 4 Lignocellulose to biofuels
Topic: enzyme technology for
conversion of lignocellulosic biomass
http://upload.wikimedia.org/wikipedia/commons/4/43/Strohr%C3%A4der.jpg7/30/2019 Umn June2011 Eijsink Web
2/8
NORWE
GIAN
UNIVERSITY
OFLIFE
SCIEN
C
ES
www.umb.no
Departm
en
tof
Ch
e
mistry,Bio
techn
olo
gyan
dFoodScien
ce
The biorefinery, 2nd generation
biofuels and enzymes Notes
Enzymatic deconstruction is the method of
choice.
Enzymes are a major cost.
Biofuel is only one of many possible products.
http://www.bioforsk.no/ikbViewer/page/forsidehttp://www.bioforsk.no/ikbViewer/page/forsidehttp://www.bioforsk.no/ikbViewer/page/forsidehttp://www.bioforsk.no/ikbViewer/page/forsidehttp://www.bioforsk.no/ikbViewer/page/forsidehttp://www.bioforsk.no/ikbViewer/page/forsidehttp://www.bioforsk.no/ikbViewer/page/forsidehttp://upload.wikimedia.org/wikipedia/commons/4/43/Strohr%C3%A4der.jpghttp://www.bioforsk.no/ikbViewer/page/forside7/30/2019 Umn June2011 Eijsink Web
3/8
NORWE
GIAN
UNIVERSITY
OFLIFE
SCIEN
C
ES
www.umb.no
Departm
en
tof
Ch
e
mistry,Bio
techn
olo
gyan
dFoodScien
ce
UMN-UMB, Team 4 specific project: CBP21,
a helper protein from the CBM33 family
Gustav Vaaje-Kolstad et al., Journal of Biological Chemistry 280: 11313 -11319 & 280:28492-28497 (2005) + Patent application
7/30/2019 Umn June2011 Eijsink Web
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NORWE
GIAN
UNIVERSITY
OFLIFE
SCIEN
C
ES
www.umb.no
4
UMN-UMB, Team 4 specific project
starting point
Goals:
Use of directed evolution (mutagenesis) to produce
accessory proteins that act on cellulose.
Generation of fundamental knowledge about how helperproteins such as CBP21 work.
People:
Claudia Schmidt-Dannert group with post-doc Jake Vick. Eijsink group with post-doc Gustav Vaaje-Kolstad and
(since 2010) Ph.D. student Zarah Forsberg.
7/30/2019 Umn June2011 Eijsink Web
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NORWE
GIAN
UNIVERSITY
OFLIFE
SCIEN
C
ES
www.umb.no
5
UMN-UMB, Team 4 specific project
Developments since 2007
GH61 proteins act synergistically with cellulose and show
structural similarity with CBM33 (CBP21)
CBM33 proteins are enzymesthat break down chitin chains
GH61 proteins are enzymesthat break down cellulose chains
Identification of natural CBM33 proteins that break downcellulose
Interesting ideas and findings at UMN concerning mechanism
CBM33 GH61
7/30/2019 Umn June2011 Eijsink Web
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NORWE
GIAN
UNIVERSITY
OFLIFE
SCIEN
C
ES
www.umb.no
Departm
en
tof
Ch
e
mistry,Bio
techn
olo
gyan
dFoodScien
ce
A new paradigm for degradation ofcrystalline polysaccharides
Oxidohydrolase(CBM33 or GH61),
catalyzing chain
cleavage in a fully
crystalline context
+Endo
Exo-processive
Vaaje-Kolstad et al., 2010,
Science 330:219-222
7/30/2019 Umn June2011 Eijsink Web
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NORWE
GIAN
UNIVERSITY
OFLIFE
SCIEN
C
ES
www.umb.no
7
Very hot project. IP issues.
Lots of mutagenesis work on CBP21 has been done andmutant characterization is in progress.
Several new, active CBM33s available.
NMR structure of CBP21 (a CBM33) has been solved.
Shift of focus from enzyme development to enzymeunderstanding.
Several joint papers, including potential breakthrough
papers on mechanism, are on their way.
Funding is running out (?)
UMN-UMB, Team 4 Status June 2011
7/30/2019 Umn June2011 Eijsink Web
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NORWE
GIAN
UNIVERSITY
OFLIFE
SCIEN
C
ES
www.umb.no
Departm
en
tof
Ch
emis
try,Bio
techn
olo
gyan
dFoodScien
ce
Notes about the future
Funding ? (currently only one PhD student at UMB)
Many applications sent in Norway (but)
MSc student exchange: Sophanit Mengesha
PhD student exchange: Zarah Forsberg (?)
Nb. Current potential is huge and progress is good, but
very high complexity (protein production, analyticaltools, theoretical biochemistry, many partners).