Ultrastructural Change in the Parathyroid Gland of the ... filewere daily given 0.4ml of 1% Na2HPO4 solution (phosphate) intraperitoneally for 3, 7, 14 and 21 days. The parathyroid

Arch. histol. jap., Vol. 33, No. 5 (1971)p. 357-370

Department of Anatomy (Prof. H. ISONO), Gifu University School of Medicine,Gifu, Japan

Ultrastructural Change in the Parathyroid Gland of thePhosphate Treated Newt, Triturus pyrrhogaster (Boie)

Hideo ISONO, Shizuko SAKURAI, Hiroyuki FUJII and Shizuyo AOKI

Received September 2, 1971

Summary. The newt parathyroid gland in natural hibernation and in hyperfunctionalconditions induced by the intraperitoneal injection of phosphate was electron-microscopi-cally studied. The average numbers of mitochondria, Golgi apparatus, large homogeneous-ly dense bodies, large heterogeneously dense bodies, vacuolar bodies and lipofuscin-likebodies were counted per 100μ2 of the cytoaplasm from the electron micrographs of the para-

thyroid glands of the phosphate treated and hibernating (control) newts.The parenchymal cells of the newt parathyroid gland were classified into basal cells

and suprabasal cells. Daily administration of phosphate caused no marked changes in theformer cells. In the latter under experimental conditions, granular and agranular endo-plasmic reticula and small dense granules seemed to be increased in number, while glycogengranules appeared decreased in number when contrasted to those in the control newts. TheGolgi apparatus and large homogeneously dense bodies were increased in number reachinga maximum after 7 days of phosphate administration, and lipofuscin-like bodies were grad-ually increased in number during the experimental stages. On the contrary, vacuolar bodieswere first rapidly, then gradually decreased in number. Large heterogeneously dense bodiessubdivided into a vesicular type and lysosomal type were slightly decreased during 14 days'administration of phosphate and increased in number after 21 day administration. Underexperimental conditions the vesicular type heterogeneously dense bodies were more domi-nant than the lysosomal type. The numbers of mitochondria were hardly varied as com-pared with control newts.

From the above results it is conceivable that alterations in cell organelles and inclusion

bodies indicate hyperfunction of the suprabasal cell caused by phosphate administration.

However, mutual correlations of inclusion bodies remain incompletely clarified.

The ultrastructure of the normal parathyroid gland has been studied extensively.Furthermore, a number of investigations on the fine structure of the parathyroid

gland under experimentally hyper- or hypo-functional conditions have been reportedin the rat (DAVIS and ENDERS, 1961; ROTH and RAISZ, 1964, 1966; LEVER, 1965;MAZZOCCHI et al., 1967; HARA and NAGATSU, 1968; ROTH et al., 1968; ROHR and KRASSIG,

1968), frog (MONTSKO et al., 1963a; LANGE and BREHM, 1965), cow (CAPEN et al., 1965),

rabbit (MELSON, 1966; TANAKA, 1969a, b; TANAKA et al., 1969a, b), mouse (STOECKELand PORTS, 1966b; NAKAGAMI, 1967), cat (CAPEN and ROWLAND, 1968) and bat (AZZALI,

1970). However, the morphology of the secretory cycle of the parathyroid gland is

still a matter of speculation.

By comparison of the parathyroid gland of the naturally hibernating newt

(SETOGUTI, ISONO and SAKURAI, 1970a) with phosphate treated ones the present paperaims at the elucidation of the correlations of different types of inclusion bodies in the

gland: small dense granules, large homogeneously dense bodies, large heterogeneous-ly dense bodies, vacuolar bodies containing uncoated vesicles and/or coated ones, and

357

358 H. ISONO, S. SAKURAI, H. FUJII and S. AOKI:

lipofuscin-like bodies (heterogeneously dense irregularly shaped masses with almost

no limiting membrane).

Materials and Methods

The experimental animals used for this work were adult newt, Triturus pyrrho-

gaster (Boie), under natural hibernation kept in a water tank at 3℃ in January. They

were daily given 0.4ml of 1% Na2HPO4 solution (phosphate) intraperitoneally for 3,7, 14 and 21 days.

The parathyroid glands from naturally hibernating (control) newts and thosetreated with phosphate were removed without anesthesia, immediately fixed inMILLONIG's fixative (1962) for 1.5 hours, dehydrated in ascending concentrations ofcold acetone, and embedded in Epon 812 (LUFT, 1961). Thin sections obtained with aPorter-Blum ultramicrotome were stained with uranyl acetate (WATSON, 1958) follow-ed, by lead acetate (MILLONIG, 1961) and examined with a JEM 6C electron microscope.

From electron micrographs taken at low magnifications of 2,000 times with a

JEM 6C electron microscope and further enlarged photographically 3.7 times, thedimensions of the cytoplasm except for the nucleus were measured by a planimeter.The average numbers per 100μ2 cytoplasm of mitochondria, Golgi apparatus, large

homogeneously dense bodies, large heterogeneously dense bodies, vacuolar bodies and

lipofuscin-like bodies were counted.

Observations

As reported previously by the present authors (SETOGUTI, ISONO and SAKURAI,1970a, b), the newt parathyroid gland consists of two main types of epithelial cells:

basal cells and suprabasal cells.

a. The basal cell

Control newts

During natural hibernation the basal cells were flat or cuboidal to polygonal inoutline and the nucleus was round, spindle or irregular shaped and often showed deep

indentations. The basal cells rested constantly on a basement membrane, and con-

tained numerous filaments, a small number of cell organelles and other inclusion

bodies; mitochondria, free ribosomes, granular and agranular endoplasmic reticula,

pinocytotic vesicles, poorly developed Golgi apparatus, glycogen granules, differentdense bodies and various vacuolar bodies (Fig. 1). In an area of peripheral cytoplasm

of the basal cell directed occasionally toward the suprabasal cells, there were observeda few filaments, numerous cell organelles and other inclusion bodies.

Phosphate treated newts

No conspicuous variations were recognized in the ultrastructure of the basal cellafter 3, 7, 14 and 21 days of phosphate administration. The fine structure under ex-

perimental conditions was about the same as that of the basal cells of control newts.

b. The suprabasal cell

Control newts

Under natural hibernation, the suprabasal cells were round, spindle or polygonalin outline, and the nucleus was round to spindle in shape and sometimes deeply in-

dented (Fig. 2). The cells were in a suprabasal position and had a few filaments,

Parathyroid Gland of the Phosphate Treated Newt 359

relatively numerous cell organelles and other inclusion bodies (Fig. 2).Mitochondria were scattered throughout the cytoplasm. The average numbers

were 50.6per 100μ2 of cytoplasm(Fig.18). Glycogen granules and free ribosomes were

relatively abundant. Granular and agranular endoplasmic reticula were diffusely

dispersed in the cytoplasm. The elements of the Golgi apparatus were dispersed in

Fig. 1. Basal cells (B) of parathyroid gland in natural hibernation. Basal cellsresting on a basement membrane (BM) contain numerous filaments, a smallnumber of cell organelles and other inclusion bodies. The nucleus (N) shows

deep indentations. ×4,500

Fig. 2. Suprabasal cells (S) of parathyroid gland in natural hibernation.Suprabasal cells have a few filaments, relatively numerous cell organelles andother inclusion bodies. The nucleus (N) shows sometimes deep indentations.

×4,500

1

2


the cytoplasm and their average numbers were 1.40per 100μ2 of cytoplasm (Fig. 18).

There were occasionally observed a few small dense granules enclosed in a loose fit-ting membrane and coated vesicles in the Golgi field. Large, round or irregularly

shaped, homogeneously dense bodies bounded by a limiting membrane were dispersed

outside or inside the Golgi fields-Their average numbers were 0.59per 100μ2 of cyto-

plasm (Fig. 18). Large, round or irregularly shaped heterogeneously dense bodieswith a limiting membrane were observed outside or inside the Golgi fields. Their

average numbers were 0.62per 100μ2 of cytoplasm (Fig. 18). They were, further-

more, subdivided into a vesicular type having mainly vesicles and a lysosomal typecontaining tubules, extremely osmiophilic flecks, and/or a less dense lipid-like sub-stance, or combinations of these. There were transitional types between the two.The lysosomal type heterogeneously dense bodies were more dominant than the

Fig. 3. Complex interdigitation of plasma membrane in adjacent suprabasal cells (S)after 7 days' phosphate administration. Lvsosomal type heterogeneously dense bodies

(LT)and vacuolar bodies (VB) are seen in the cytoplasm. ×5,000

Fig. 4. Relatively wide intercellular space surrounded by four suprabasal cells (S)containing a large amount of floccular substance after phosphate administration. ×4,700

Fig. 5. Small dense granules observed along cellular membrane after phosphate admin-

istration. ×3,500

Fig. 6. Portion of a suprabasal cell after phosphate administration. In the Golgi field(G), moderately dense material containing vesicles mingled with a few coated ones andsmall dense granule are observed. ×20,100. Inset. Fairly large-sized dense granule.

×32,000

Fig. 7. Round homogeneously dense bodies (HmB) and lipofuscin-like bodies (LB) seeninside and outside the Golgi field (G) in a suprabasal cell after phosphate administration.

×7,000

3

4

5

6

7


vesicular type, and most of the heterogeneously dense bodies consisted of the lysosomaltype. In addition, large vacuolar bodies with a round or oval, sometimes irregularlyshaped outline, containing various numbers of uncoated vesicles and/or coated ones,and frequently a floccular or extremely dense material, were frequently dispersed out-side or inside the Gobi fields. They averaged 5.57per 100μ2 of cytoplasm (Fig. 18).

Furthermore, lipofuscin-like bodies containing an extremely osmiophilic substance,

several tubular structures, and a homogeneously less dense lipid-like material were

Fig. 8. Vesicular type heterogeneously dense bodies (VT) having small vesicles, and vacuolarbodies (VB) containing vesicles and floccular material seen in small groups in cytoplasm of

suprabasal cell after phosphate administration. ×16,900

Fig. 9. Lysosomal type heterogeneously dense bodies (LT) containing tubular structuresand extremely osmiophilic material, vesicular type heterogeneously dense bodies (VT) havingsmall vesicles. and vacuolar bodies (VB) containing vesicles and floccular material observedin small groups in the cytoplasm of suprabasal cell after phosphate administration. ×14,300

Fig. 10. Irregularly shaped vacuolar body (VB) containing vesicles and extremely densematerial in suprabasal cell after phosphate administration. ×17,600

Fig. 11. Round or oval, sometimes irregularly shaped homogeneously dense bodies (HmB),vesicular type heterogeneoualy dense bodies (VT) having small vesicles, and lysosomal typeheterogeneously dense bodies (LT) containing an extremely osmiophilic material dispersedin large groups in the cytoplasm of suprabasal cell after 7 days' phosphate administration.A large number of vacuoles are seen in the peripheral cytoplasm and along the cellular

membrane near the intercellular space. ×13,500

8

9

10


observed mainly in the Golgi fields and had a limiting membrane. They averaged

0.58per 100μ2 of cytoplasm (Fig. 18). Sometimes there were intermediate types

between the lipofuscin-like bodies and the large heterogeneously dense bodies.

Phosphate treated newtsIn the parathyroid glands after 3, 7, 14 and 21 days of phosphate administration,

the cytoplasm in the majority of the suprabasal cells comprised a greater proportionof the total cell volume than was observed in control newts. After 7 and 14 days of

phosphate administration, the plasma membrane between adjacent suprabasal cellsshowed complex interdigitation (Fig. 3). Intercellular spaces were generally narrowerthan those in control newts and contained a floccular substance in various quantities,but intercellular spaces surrounded by three or more suprabasal cells were, in some

places, relatively wide and contained a large amount of floccular substances (Fig. 4).The nucleus had slight indentations, and its surfaces were generally smoother thanthose in control newts, The average numbers of mitochondria were 50.4per 100μ2

of cytoplasm after 3 days of phosphate administration, 48.5 after 7 days, 58.8 after 14

days and 45.6 after 21 days (Fig. 18). Glycogen granules and free ribosomes were

dispersed in the cytoplasm and the former were decreased in number when compared

with control newts. Granular and agranular endoplasmic reticula were distributed

Fig, 12-15. Various types of lipofuscin-like bodies (LB) with almost no limiting membranecomposed of an extremely osmiophilic substance and a homogeneously less dense lipid-likematerial observed in the cytoplasm of the suprabasal cell after phosphate administration.

Fig. 12: ×25,400, Fig. 13: ×18,400, Fig. 14: ×20,100, Fig. 15: ×22,800

Fig. 16. Increased lipid droplets in suprabasal cell after phosphate administration. ×12,900

Fig. 17. Intermediate type between lipofuscin-like body and lysosomal type heterogeneous-ly dense body. ×14,000

12

13

14

15

16

17


randomly in the cytoplasm and seemed to be somewhat increased in number whencompared with those in control newts.

The elements of the Golgi apparatus were distributed widely throughout the

cytoplasm. They consisted of two to four elongated membranous cisternae, vacuoles

increased in size and number, and numerous vesicles containing a moderately densematerial mingled with a few coated vesicles (Fig. 6, 8). There were observed a large

number of small dense granules and a small number of fairly large-sized dense

granules enclosed in a loose fitting membrane in the Golgi fields (Fig. 6, 8). The aver-age numbers of the elements of the Golgi apparatus were 2.10per 100μ2 of cytoplasm

after 3 days of phosphate administration, 2.44 after 7 days, 1.99 after 14 days and 1.92after 21 days (Fig. 18). Furthermore, as contrasted with the Golgi apparatus in controlnewts, they were increased in size and complexity in the majority of the suprabasalcells. Occasionally, small dense granules resembling those seen in the Golgi fieldswere observed in the peripheral cytoplasm or along the cellular membrane (Fig. 5).

Both inside and outside the Golgi fields, large, round or oval, sometimes irregular-ly shaped homogeneously dense bodies bounded by a limiting membrane (Fig. 7),

Fig. 18. Changes in average numbers of cell organelles and other inclusion bodies per 100μ2 of the

cytoplasm of the suprabasal cells after phosphate administration. M mitochondria, G Golgi appara-

tus, MB homogeneously dense bodies, TB heterogeneously dense bodies, V vacuolar bodies, LB lipo-fuscin-like bodies.


large, round, or oval sometimes irregularly shaped heterogeneously dense bodies sub-divided into a vesicular type having mainly small vesicles (Fig. 8, 9) and a lysosomaltype containing tubular structures and an extremely osmiophilic material (Fig. 2, 9),and large, round or oval, sometimes irregularly shaped vacuolar bodies containingvesicles and/or coated ones and a floccular or extremely dense material (Fig. 2, 8-10)were frequently dispersed in small or large groups (Fig. 11). There were, of course,transitional types among the four mentioned above. In the phosphate treated newts,the vesicular type heterogeneously dense bodies were more dominant than the lyso-somal type. The average numbers of the large homogeneously dense bodies were1.45per 100μ2 of cytoplasm after 3 days of phosphate administration, 2.48 after 7 days,

1.80 after 14 days and 1.60 after 21 days (Fig. 18). The average numbers of the largeheterogeneously dense bodies were 0.55 after 3 days, 0.49 after 7 days, 0.53 after 14days and 1.08 after 21 days (Fig. 18). The average numbers of the vacuolar bodieswere 3.41 after 3 days, 3.20 after 7 days, 2.89 after 14 days and 2.43 after 21 days (Fig.18).

Lipofuscin-like bodies with almost no limiting membrane, composed of an ex-tremely osmiophilic substance and homogeneously less dense lipid-like material (Fig. 7,12-15), and lipid-droplets (Fig. 16) were frequently observed, mainly in the Golgi fields.The average numbers of the former were 0.69per 100μ2 of cytoplasm after 3 days of

phosphate administration, 1.08 after 7 days, 0.95 after 14 days and 1.26 after 21 days(Fig. 18). The latter appeared to be increased in number when compared with thecontrol newts. Sometimes there were intermediate types between the lipofuscin-likebodies and lysosomal type heterogeneously dense bodies (Fig. 17), as observed in con-trol newts.

A large number of vacuoles similar to those investing the Golgi fields were often

present in the peripheral cytoplasm adjacent to the Golgi field and along the cellularmembrane near the intercellular spaces (Fig. 7, 11).

Discussion

The ultrastructural observations of the amphibian parathyroid glands have beendescribed by several workers (Bufo vulgaris: HARA et al., 1959; LANGE and BREHM,1965; Rana esculenta: MONTSKO et al., 1963a, b; TIGYI et al., 1968; Rana temporaria:LANGE and BREHM, 1965; Rana clamitans: ROGERS, 1965; Rana pipiens: CORTELYOU andMCWHINNIE, 1967; Xenopus laevis: COLEMAN, 1969). Only a single cell type has beenidentified in the amphibian parathyroid glands. As reported previously by the presentauthors (SETOGUTI et al., 1970a, b), however, also in the present study on the newt

parathyroid gland the basal cells and the suprabasal cells were distinguishable in theparenchyma. The cell organelles and other various inclusion bodies in the basal cellsafter phosphate administration did not show much change.

The fine structure of the parathyroid gland after the administration of phosphatehas been stated by some authors (LANGE and BREHM, 1965; MELSON, 1966; STOECKELand PORTE, 1966b; ROTH et al., 1968). In the suprabasal cells stimulated by daily in-

jections of phosphate, there were recognized marked differences in shape, number andsize of cell organelles and other inclusion bodies as compared with the control animals.

The plasma membranes of the suprabasal cells pursued a tortuous course, showingcomplex interdigitations between contiguous cells after 7 and 14 days of phosphate


administration. Similar changes in the plasma membrane have been reported in activechief cells of the parathyroid glands (DAVIS and ENDERS, 1961; ROTH and MUNGER,1962; ROTH and RAISZ, 1964; CAPEN and YOUNG, 1967; CAPEN and ROWLAND, 1960.

The numbers of mitochondria were hardly varied as compared with controlnewts. However, a decreased number of glycogen granules, an increased number of

granular and agranular endoplasmic reticula and an increased number of well devel-oped Golgi apparatus (a maximum after 7 days of phosphate administration) as com-

pared with control newts may show a hyperfunctional condition of the suprabasalcells as reported by several authors (CAPEN et al., 1965; MELSON, 1966; ROTH and RAISZ,1966; MAZZOCCHI et al., 1967).

In the Golgi fields there were numerous small dense granules reported generally

as prosecretory granules in the parathyroid gland cells. They were also present,although less numerous, in the peripheral cytoplasm outside the Golgi fields. Such

small dense granules were increased in number as compared with the control newts.

This increase is thought to imply an active secretory function of the suprabasal cellsunder experimental condition. LEVER (1965), MELSON (1966) and NEVALAINEN (1969)

have reported the possibility that in the actively secreting parathyroid gland of the

rat, rabbit and hen, respectively, small prosecretory granules most frequently found

in the Golgi fields could be discharged out of the cells without being coalesced intolarge, mature storage secretory granules. Recently, YOUSHAK and CAPEN (1970) have

described that in the hyperactive chief cells of the chicken parathyroid gland pro-secretory granules budding from the Golgi membranes fuse with the plasma mem-

brane and their internal cores appear to be discharged through a hiatus in the plasma

membrane into the extracellular space.

In addition, most large, round or oval membrane-bounded homogeneously dense

bodies found outside or inside the Golgi fields, resembled those hitherto reported as

being secretory granules in the parathyroid glands. However, some of them, especial-ly the irregularly shaped homogeneously dense bodies, were considered to be lysosomal

bodies, and such dense bodies were only rarely observed in experimental newts, though

they were frequently found in control newts. The large homogeneously dense bodies

were increased in number as compared with control newts, reaching a maximum after7 days of phosphate administration. It has been described that secretory granules

increased in number in the parathyroid glands upon experimental stimulation (DAVIS

and ENDERS, 1961; ROTH and RAISZ, 1964, 1966; CAPEN et al., 1965).

The large, round or irregularly shaped heterogeneously dense bodies subdividedinto a vesicular type and lysosomal type were present outside or inside the Golgi field

and there were transitional types between the two. In control newts, lysosomal type

heterogeneously dense bodies were more dominant than the vesicular type, and most

of the large heterogeneously dense bodies consisted of the lysosomal type. On the

contrary, in this experiment showing a hyperfunction of the suprabasal cells, thelatter were more dominant than the former, and the large heterogeneously dense

bodies were slightly decreased in number until after 14 days of phosphate administra-

tion, increasing at 21 days as compared with control newts. This finding is thought

to indicate that the vesicular type heterogeneously dense bodies are functionally dif-

ferent from the lysosomal type. Function of the former showing an intermediate stagebetween the homogeneously dense bodies and vacuolar bodies was unconfirmable, but


the latter seemed to be transformed into vacuolar bodies and seemed to display typical

lysosomal digestion processes. Large vacuolar bodies were also observed both outside

and inside the Golgi field. Of these, the vacuolar bodies containing small vesiclesbelong to the so-called multivesicular bodies described in several animal species (DAVIS

and ENDERS, 1961; HARA and NAGATSU-ISHIBASHI, 1964; LANGE and BREHM, 1965;

ROGERS, 1965; NAKAGAMI, 1967; CORTELYOU and MCWHINNIE, 1967; HARA and NAGATSU,1968; NAKAGAMI et al., 1968). In this work, the vacuolar bodies were first rapidly,

then gradually decreased in number during experimental states. Their decrease

might be closely related to a hyperfunctional condition of the suprabasal cells. Thevacuolar bodies suggested to be lysosomal in nature in the previous reports (SETOGUTI,

et al., 1970a, b) on the basis of the theses of GORDON et al. (1965), SMITH and FARQUHAR

(1966), HOLTZMAN et al. (1967) and LANE (1968) are thought to be derived from thehomogeneously dense bodies through vesicular or lysosomal type heterogeneously

dense bodies though there are functional differences between both, since there were

transitional forms of the vacuolar bodies and heterogeneously dense bodies, and these

bodies frequently appeared in company with each other.

Different speculations have been made on the mechanism of the extrusion ofsecretory granules from chief cells. Many investigators have described that the limit-

ing membrane of the secretory granule fuses with the plasma membrane (STOECKEL

and PORTS, 1966a; MELSON, 1966; NAKAGAMI, 1967; CAPEN and YOUNG, 1967; TANAKA,1969b; YOUSHAK and CAPEN, 1970). Other workers have considered dense granules in

the connective tissue space and the capillary endothelial cell as secretory granules

extruded from chief cells (MUNGER and BOTH, 1963; CAPEN et al., 1965; MELSON, 1966;FETTER and CAPEN, 1970). Furthermore, STOECKEL and PORTE (1966a, b) and TANAKA

(1969b) have suggested that secretory granules pass into the extracellular space in asoluble form after fusing with the plasma membrane. In the present study, however,the large round homogeneously dense bodies resembling secretory granules which

attach to the plasma membrane and are in the extracellular space, were not observed.

Accordingly, the morphological evidence of the mechanism by which the large round

homogeneously dense bodies are extruded from the suprabasal cells was unconfirm-able.

Lipofuscin-like bodies and lipid droplets were observed mainly in the Golgi fields.

Similar bodies have been reported in the parathyroid glands of several mammals

(LANGE, 1961; MUNGER and ROTH, 1963; NAKAGAMI, 1965; CAPEN et al., 1965; ROHR andKRASSIG, 1968; CAPEN and ROWLAND, 1968; FETTER and CAPEN, 1970). NAKAGAMI (1965)

has supposed that some of such bodies may be lipid and some others lysosomal and

ROHR and KRASSIG (1968) have stated that they may be a hormone-lipoprotein complex

and transformed into lipids after the release of the hormones. FRANK and CHRISTENSEN

(1968) have reported that lipofuscin pigments show acid phosphatase activity in theirmatrix. Accordingly, lipofuscin-like bodies may be lysosomal in nature as described

by STREHLER and MILDVAN (1962), BARKA and ANDERSON (1963), KOENIG (1963), and

SAMORAJSKI et al. (1965). It is presumed, furthermore, that these lipof uscin-like bodies

may be end products of the degeneration of various organelles in the Golgi fields as

it has been reported that lipofuscin pigments are derived from the Golgi bodies

(GATENBY and MOUSSA, 1951; BONDAREFF, 1957) and ultimately they may be changedinto lipid droplets. WILCOX (1959) has suggested that the more physiologically active


neurons may accumulate lipofuscin at a faster rate than metabolically less active

cells. In this research a large number of lipofuscin-like bodies derived from the

Golgi apparatus which were increased in number in the cytoplasm by phosphateadministration were observed in the active suprabasal cells.

Acknowledgement. The authors wish to acknowledge the technical assistance of Mr. T. KATORI.

燐投与のアカハライモリ上皮小体の電子顕微鏡的研究

磯野日出夫, 桜井静子, 藤井寛之, 青木静代

自然冬眠下 (対照) および燐投与のアカハライモリ上皮小体を電子顕微鏡で観察した.

なおミトコンドリア, ゴルジ装置, 均質暗調小体, 不均質暗調小体, 空胞様小体および

リポフスチン様小体の細胞質100μ2あたりの平均値が算出された.

アカハライモリ上皮小体の実質細胞は, フィラメントが多く細胞小器官に乏しい basal

cell (基底細胞) とフィラメントに乏しく細胞小器官の発育良好な suprabasal cell (基

底上細胞) の2種類に区別される.

基底細胞では少数の細胞小器官と封入体には, 対照と燐投与の間に大差はない.

基底上細胞は実質の大部分を占め, 燐投与のものでは細胞質は肥大し, 小胞体は増加

し, グリコゲン顆粒は減少する. ミトコンドリアは対照と燐投与の間にほとんど差は認

められない. ゴルジ装置は対照に比して増加し, 燐投与7日に最高値を示し, ゴルジ野

における小暗調顆粒 (分泌前顆粒と考えられる) も増加する. 均質暗調小体 (分泌顆粒

を含むと考えられる) は対照に比して増加し, 燐投与7日に最高値を示す. 不均質暗調

小体 (小胞型とリゾソーム型に区別) は燐投与14日までは対照に比してやや減少するが,

燐投与21日では増加する. 対照ではその大部分がリゾソーム型からなるが, 燐投与では

小胞型が優勢である. 空胞様小体は燐投与3日に急激に減少し, 以後漸減する. リポフ

スチン様小体は対照に比して漸増する.

以上の所見から燐投与による細胞小器官と封入体の変動は基底上細胞の機能亢進状

態を示すと考えられるが, 封入体の相互関係はさらに検討を要する問題であろう.

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磯野日出夫

〒500岐阜市司町40

岐阜大学医学部

第一解剖学教室

Prof. Hideo ISONODepartment of Anatomy

Gifu University School of Medicine500 Gifu, Japan

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Ultrastructural Change in the Parathyroid Gland of the ... filewere daily given 0.4ml of 1% Na2HPO4 solution (phosphate) intraperitoneally for 3, 7, 14 and 21 days. The parathyroid