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Clarus 500 Gas Chromatograph/Mass Spectrometer
ultimate data integrity
innovative technology
Unmatched accuracy and precision
The Clarus® 500 Gas Chromatograph/Mass Spectrometer (GC/MS) features a wealth of innovative technology to provide the most complete characterization of samples. PerkinElmer Selected Ion and Full Ion (SIFI™) scanning technology and high scan speed increase system throughput and accuracy. The mass spectrometer boasts the fastest, widest-mass-rangequadrupole mass spectrometer available for GC/MS, providing capability to analyze a greater scope of applications. The Clarus 500 GC/MS also includes proven gold technology for enhanced performance.
State-of-the-art electronics process more scans acrosseach peak, resulting in unmatched accuracy and preci-sion and better overall spectral fidelity. Couple the highperformance electronics with unique productivity toolsand you have a system that provides the functionalitynecessary to achieve optimum performance, allowingyou to complete work quickly, easily and efficiently.
The wide mass range and flexible data acquisitionmodes help tackle the most complex applications,while robust instrument design delivers maximumuptime, easy maintenance and lower operating cost.
Combine the Clarus 500 MS with the Clarus 500 GC,market-leading PerkinElmer sample handling acces-sories, flexible user-friendly software and world-classservice and support for an integrated, complete analyticalsolution. Whatever your application – environmental,chemical, flavor and fragrance, food, beverage, forensicor pharmaceutical – PerkinElmer delivers the accuracyand precision you need.
Run fast GC using GC/MS
With faster scan speeds, the Clarus 500 GC/MS opensnew and attractive opportunities in fast GC applications.Extremely narrow chromatographic peaks, previouslyunresolvable with full-scan quadrupole mass spectro-metry, can be easily defined and quantified with theClarus 500 GC/MS (Figure 1). Consider the productivitygains your lab can achieve by applying fast GC/MStechniques, such as in separation of solvent mixtures or semivolatile components.
2
Figure 1. In fast GC/MS, the Clarus 500 GC/MS resolves peaksthat were previously unresolvable with full scan quadrupolemass spectrometry. The top chromatogram shows resolutionwith slow scan speed, while the lower chromatogramdemonstrates the benefit of increasing the number of scans.
the choice for high performance
Take the lead on GC/MS speed
The Clarus 500 GC/MS is the fastest quadrupole GC/MSavailable, acquiring more spectra (up to 60 scans/second)across a GC peak than any other GC/MS system. Morespectra per peak means more accurate and preciseresults (Figure 2) and better spectral fidelity (Figure 3),resulting in the most complete information and thehighest confidence in analytical data reporting.
www.perkinelmer.com 3
Figure 2. Chromatographic signal at fast scan speed (red) compared to slow scan speed (green).
How does fast scan speed improveyour data?
Scan speed is the rate of data acquisition across a
chromatographic peak, measured as scans per second.
Scan speed of a quadrupole mass spectrometer
is critical in ensuring data integrity. Too few scans
across a GC peak lead to poor recorded peak shape,
limiting quantitative accuracy and precision (Figure 2).
A slow scan rate on a fast peak causes spectral
distortion, or “skewing”, reducing spectral fidelity
(Figure 3, top). Faster scanning minimizes the
skewing and gives better overall spectral fidelity
(Figure 3, bottom).
The Clarus 500 GC/MS incorporates fast micro-
processor technology bringing new, high-speed,
state-of-the-art electronics to GC/MS data
processing that maximizes scan speed. Up to 60
scans/second can be achieved, the fastest scan
speed available in quadrupole GC/MS. The result
is the most accurate and precise data ever, easily
beating the generally accepted criteria of at least
8-10 data points per chromatographic peak, for
performance unmatched by other GC/MS systems.
Figure 3. Better spectral fidelity with fast scan speeds,increasing confidence in results. Top and bottom figure withspectra acquired from (1) fronting, (2) apex and (3) tailing pointalong a chromatographic peak.
Slow Scan Speed
Fast Scan Speed
(1)
(2)
(3)
(1)
(2)
(3)
innovative tools bring
productivity to new levels
The Clarus 500 GC/MS features innovative and uniqueproductivity-enhancing tools that help laboratoriesoperate effectively and efficiently.
Selected Ion and Full Ion (SIFI) scanning
The Clarus GC/MS can perform a powerful processcalled Selected Ion and Full Ion (SIFI) scanning in whicha Selected Ion Monitoring (SIM) scan is obtained whilesimultaneously acquiring data in the Full Scan mode.Since SIFI provides the information from both opera-tional modes in one chromatographic run, you receivemore information in less time. With other GC/MS
systems, two or more runs might be required to accom-plish the same task.
With SIFI, labor-intensive pre-concentration and sampleclean-up steps may be reduced or eliminated, saving timeand money on costly solvents. In addition, compoundsdifficult to determine at low levels, such as penta-chlorophenol, can be detected and quantified with greateraccuracy and greater sensitivity. This also reduces thenumber of analyses by combining a wider range ofanalyte responses in a single chromatographic run,improving the throughput of your lab.
4
What is SIFI scanning?
The Clarus 500 GC/MS can perform a Selected Ion
Monitoring (SIM) scan while simultaneously acquiring
data in the Full Scan mode (Figure 4). Called SIFI
scanning, this unique technique provides more
complete sample information in less time.
The SIM mode increases sensitivity by selectively
scanning for individual masses, while weeding out or
ignoring unwanted or irrelevant masses. This powerful
scan mode focuses all the attention on masses of
interest, without wasting effort and efficiency. By using
SIM, detection limits can be extended down to the
femtogram range. In Full Scan mode, low picogram
levels can be detected, allowing acquisition of library-
searchable spectra over a wide concentration range.
SIFI scanning ensures accurate identification, while
simultaneously providing enhanced quantifiable
sensitivity from the selected ion signal.
SIFI is NOT an extracted chromatogram from a full scan
acquisition. Extracted ion chromatograms offer no
improvement in signal strength for the ions of interest –
only noise rejection from the unwanted masses. SIFI
provides at least an order of magnitude improvement
in detection limits.
SIFI is also a powerful way to troubleshoot unknowns
during routine quantification due to its ability to provide
accurate and complete sample information.
Figure 4. SIFI scanning ensures accurate identification, whilesimultaneously providing enhanced quantifiable sensitivityfrom the selected ion signal.
The software uses Microsoft® Windows® 2000, providing a high level of security, network connectivity andoperating stability. In addition, different levels of useraccess can be defined to ensure data integrity by securingfiles from unauthorized modification. Mass spectral datacan be imported from or exported to industry standardAIA format. A variety of mass spectral libraries forcompound identification, including NIST, Wiley andPfleger/Maurer/Weber, are available.
Compound:THC-Acid (deriv)
Retention Time:4.596 min
Concentration:4.038 ng/mL
Pass/Fail:
Pass
Target
Qualifier 1
Qualifier 2
Overlay
4.404.60
4.80
Time
0
100
%
drugs_61
SIR of 6 Channels EI+
371.00
1.51e6
4.60
4.404.60
4.80
Time
0
100
%
drugs_61
SIR of 6 Channels EI+
473.00
6.27e5
4.60
4.404.60
4.80
Time
0
100
%
drugs_61
SIR of 6 Channels EI+
488.00
3.02e5
4.60
Compound 1 name: THC-Acid (deriv)
Correlation coefficient: r = 0.999993, r^2 = 0.999986
Calibration curve: 1.22007 * x + 0.122247
Response type: Internal Std ( Ref 3 ), Area * ( I
S Conc. / IS Area )
Curve type: Linear, Origin: Exclude, Weighting: Null, Axis trans: None
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0Conc
0
6.23
Response
Signature _____________________________________
Date ________________
*** Analyte #1: TH C-Acid (d eriv) E
xpected RT = 4.600
Rt (min)
m/z
Area
Time RangeRatio Range
RatioP/F
ng/mL
4.596371.2
1,855,395.7
4.504 - 4.688
100
100. Pass
4.038
4.596473.3
244,108.5
4.504 - 4.688 10.8 - 16.3
13.1
Pass
4.596488.3
82,158.2
4.504 - 4.688 3.5 - 5
.3
4.4
Pass
4.404.60
4.80
Time
0
100
%
drugs_61
SIR of 6 Channels EI+
1.51e6
4.60
Data File:c:\TurboMass\Drugs031803.PRO\Data\drugs_61.raw
Acquired:18-Mar-2003, 11:39:41 AM
Vial Number: 20
Page:1 of 2
Sample ID:Drug std A1, inj 5
Printed: 18-Mar-2003, 11:42:23 AM
Misc. Info:1@140-270@10, split 50:1
Tune File:031803.ipr
18-Mar-2003, 9:11:12 AM
GC Method:THC07.mth
2-Feb-2003, 4:55:02 PM
MS Method:THC07.exp
2-Feb-2003, 4:56:13 PM
Calibrated:THC180303.cdb
18-Mar-2003, 8:12:22 AM
Quantitation Report
Hamden Forensic Labs
Area % and Norm % Chrom atogram Report
Hamden Drug Labs
Page 1 of 3
26-Aug-1997 + 16:59:50
Unleaded gasoline
2.004.00
6.008.00
10.0012.00
14.0016.00
18.00Time
0
100
%
GAS2
Scan EI+
TIC
8.73e6
1.97;91
1.37
41
7.87
105
7.05
1054.11
912.14
573.81
91
3.13;57
4.82
916.82
915.26
43
9.73
1198.43
105
9.23
119
11.46
12810.85
117
13.16
14212.46
131
14.84
156
#RT
ScanHeight
AreaArea %
Norm %
11.035
7 3,487,782
90,179.4
1.8%23.8%
21.110
22 1,155,053
31,141.7
0.6%8.2%
31.170
34 5,478,535
200,068.5
4.1%52.7%
41.225
45 7,207,080
271,936.8
5.5%71.6%
51.291
58 7,303,899
281,044.8
5.7%74.0%
61.365
73 3,432,642
196,023.3
4.0%51.6%
71.445
89933,112
26,700.0
0.5%
7.0%
81.550
110 2,118,722
159,196.0
3.2%41.9%
91.685
137519,861
16,518.6
0.3%
4.4%
101.770
154 2,573,187
90,637.3
1.8%23.9%
111.836
167 1,490,023
64,787.3
1.3%17.1%
121.966
193 7,845,757
317,822.9
6.4%83.7%
132.026
205 1,931,275
67,752.2
1.4%17.8%
142.136
227 2,265,743
73,585.4
1.5%19.4%
152.296
259454,916
31,789.5
0.6%
8.4%
162.426
285 1,419,400
53,088.1
1.1%14.0%
172.556
311766,284
39,674.5
0.8%
10.4%
182.671
334464,081
17,065.8
0.3%
4.5%
193.132
426701,987
27,957.9
0.6%
7.4%
203.807
561 2,022,641
104,038.7
2.1%27.4%
213.937
587416,862
19,812.4
0.4%
5.2%
224.112
622 4,261,270
379,674.1
7.7%100.0%
234.207
641 1,112,457
62,130.5
1.3%16.4%
244.352
670245,227
27,033.7
0.5%
7.1%
254.818
763 2,451,974
129,953.0
2.6%34.2%
265.258
851439,575
31,690.8
0.6%
8.3%
275.464
892235,164
14,362.5
0.3%
3.8%
285.949
989264,089
10,447.5
0.2%
2.8%
File:c:\TurboMass\Drugs031803.PRO\Data\BNAstd26c3.raw
Acquired: 18-Mar-2003, 11:39:41 AM
Printed: 18-Mar-2003, 11:42:23 AM
Sample ID: Drug std A1, inj 5
Page: 1 of 2
Misc. Info: 1@140-270@10, splits 50:1
Vial Number: 20
Inst(Clarus 500 MS #1) ACQUISITION PARAMETERS:
Oven: Initial Temp 140”C for 1 min, ramp 10.0”C/min to 270”C, hold 1 in, InjAauto=270”C, Volume=1.00 L
Split=50:1, Carrier Gas=Helium, Solvent Delay-2.50 min, Transfer Temp=200”C, Source Temp=150”C
Scan: 40 to 270Da, Column: 25m x 0.25mm x 0.25 m PE-5MS methyl silicone
1: Isopherone
2: Hexachlorocyclopentadiene3: Dimethyl phthalate
4: Acenapthylene
5: 2,6-dinitrotoluene6: 2,4-dinitrotoluene
7: 2-Chlorobiphenyl8: Acenaphthene-d10
9: Diethyl phthalate
10: Fluorene
11: 2,3-Dichlorobiphenyl12: Hexachlorobenzene
13: Pentachlorophenol14: Phenanthrene-d10
15: Phenanthrene
16: 2,4,5-trichlorobiphenyl
17: 2,2’,4,4’-tetrachlorobiphenyl 18: Pyrene
19: Di-n-butyl phthalate20: 2,2’,3’,4,6-pentachlorobiphenyl
21: 2,2’,3,3’,4,4’,6-heptachlorobip... 22: 2,2’,4,4’,5,6’-hexachlorobiph... 23: Benz(a)anthracene24: Anthracene
File:c:\TurboMass\Drugs031803.PRO\Data\BNAstd26c3.raw
Acquired: 18-Mar-2003, 11:39:41 AM
Printed: 18-Mar-2003, 11:42:23 AM
Sample ID: Drug std A1, inj 5
Page: 1 of 2
Misc. Info: 1@140-270@10, splits 50:1
Vial Number: 20
Quantific atio n Ion Integratio n Summary Report
Hamden Enviro nmental L abs
Inst(Clarus 500 MS #1) ACQUISITION PARAMETERS:
Oven: Initial Temp 140”C for 1 min, ramp 10.0”C/min to 270”C, hold 1 in, InjAauto=270”C, Volume=1.00 L
Split=50:1, Carrier Gas=Helium, Solvent Delay-2.50 min, Transfer Temp=200”C, Source Temp=150”C
Scan: 40 to 270Da, Column: 25m x 0.25mm x 0.25 m PE-5MS methyl silicone
0.902.90
4.90Time
0
100
%
BNAstd26c3
Scan EI+
821.78e4
2.92
1.953.95
5.95Time
0
100
%
BNAstd26c3
Scan EI+
2373.10e3
3.97
2.754.75
6.75Time
0
100
%
BNAstd26c3
Scan EI+
1632.06e4
4.77
3.005.00
7.00Time
0
100
%
BNAstd26c3
Scan EI+
1523.14e4
5.00
2.854.85
6.85Time
0
100
%
BNAstd26c3
Scan EI+
1652.00e4
4.88
3.555.55
7.55Time
0
100
%
BNAstd26c3
Scan EI+
892.73e3
5.61
3.305.30
7.30Time
0
100
%
BNAstd26c3
Scan EI+
1882.00e4
5.33
3.205.20
7.20Time
0
100
%
BNAstd26c3
Scan EI+
1641.08e5
5.22
4.056.05
8.05Time
0
100
%
BNAstd26c3
Scan EI+
1491.98e4
6.07
4.256.25
8.25Time
0
100
%
BNAstd26c3
Scan EI+
1662.03e4
6.28
5.507.50
9.50Time
0
100
%
BNAstd26c3
Scan EI+
2228.41e3
7.51
5.507.50
9.50Time
0
100
%
BNAstd26c3
Scan EI+
2845.34e3
7.56
6.108.10
10.10Time
0
100
%
BNAstd26c3
Scan EI+
2665.48e3
8.14
6.608.60
10.60Time
0
100
%
BNAstd26c3
Scan EI+
1881.65e5
8.63
6.708.70
10.70Time
0
100
%
BNAstd26c3
Scan EI+
1783.71e4
8.70
7.259.25
11.25Time
0
100
%
BNAstd26c3
Scan EI+
2587.41e3
9.37
8.9010.90
12.90Time
0
100
%
BNAstd26c3
Scan EI+
2206.34e3
10.91
11.3013.30
15.30Time
0
100
%
BNAstd26c3
Scan EI+
2024.59e4
13.32
8.8010.80
12.80Time
0
100
%
BNAstd26c3
Scan EI+
1494.16e4
10.81
10.4012.40
14.40Time
0
100
%
BNAstd26c3
Scan EI+
3265.59e3
12.38
15.8017.80
19.80Time
0
100
%
BNAstd26c3
Scan EI+
3943.65e3
17.78
12.2014.20
16.20Time
0
100
%
BNAstd26c3
Scan EI+
3606.06e3
14.19
15.8517.85
19.85Time
0
100
%
BNAstd26c3
Scan EI+
2283.73e4
6.858.85
10.85Time
0
100
%
BNAstd26c3
Scan EI+
1783.71e4
8.85
Intuitive software makes analysis easy
The Clarus 500 GC/MS runs on PerkinElmer’s provenTurboMass™ software platform (Figure 5), which offersthe versatility needed to run both routine and complexapplications. TurboMass software for the Clarus 500GC/MS makes report creation both intuitive and quick.The software includes report templates (Figure 6)designed to meet the specific needs of users conductingforensic, clinical or toxicological diagnostics, as well as general chemical analysis.
Easy to learn
The sample-centric graphical user interface, AutoTune,and other data-processing features are powerful yeteasy to master, even for less skilled users. With a shortlearning curve, staff is easily trained and productiveright away. The software includes flexible reports thatcan be generated quickly and easily. Data is easilyaccessible using our powerful Visual Basic™-basedmacro language and can be exported to spreadsheetand database programs.
www.perkinelmer.com 5
Figure 5. Powerful, easy-to-usesoftware with sample-centric graphical user interface.
1: d3-THC-Acid (d eriv ) IS
2: THC-Acid (deriv)
4.30
4.35
4.40
4.45
4.50
4.55
4.60
4.65
4.70
4.75
4.80
4.85
4.90Time
0
100
%
drugs_61
SIR of 6 Channels EI+
TIC7.04e6
4.60
Data File:
c:\TurboMass\Drugs031803.PRO\Data\drugs_61.raw
Acquired:
18-Mar-2003, 11:39:41 AM Vial Number: 20
Page:1 of 2
Sample ID:Drug std A1, inj 5
Printed: 18-Mar-2003, 11:42:23 AM
Misc. Info:1@140-270@10, split 50:1
Tune File:
031803.ipr
18-Mar-2003, 9:11:12 AM
GC Method:THC07.mth
2-Feb-2003, 4:55:02 PM
MS Method:THC07.exp
2-Feb-2003, 4:56:13 PM
Calibrated:THC180303.cdb
18-Mar-2003, 8:12:22 AM
Quantita tio n Report
Hamden Forensic Labs
Signature _____________________________________
Date ________________
4.404.60
4.80 Time
0
100
%
drugs_61SIR of 6 Channels EI+3.99e5
4.59
4.404.60
4.80 Time
0
100
%
drugs_61SIR of 6 Channels EI+1.51e6
4.60
4.404.60
4.80 Time
0
100
%
drugs_61SIR of 6 Channels EI+
374.003.99e5
4.59
4.404.60
4.80 Time
0
100
%
drugs_61SIR of 6 Channels EI+
476.001.79e5
4.59
4.404.60
4.80 Time
0
100
%
drugs_61SIR of 6 Channels EI+
491.008.62e4
4.59
4.404.60
4.80 Time
0
100
%
drugs_61SIR of 6 Channels EI+
371.001.51e6
4.60
4.404.60
4.80 Time
0
100
%
drugs_61SIR of 6 Channels EI+473.006.27e5
4.60
4.404.60
4.80 Time
0
100
%
drugs_61SIR of 6 Channels EI+
488.003.02e5
4.60
** Internal Standa rd of #1: d3-THC-Acid (deriv) IS Expected RT = 4.594
Rt (min)m/z
Area
Time Range
Ratio RangeRatio
P/F ng/mL
4.590374.2
700,371.2
4.498 - 4.682
100
100. Pass
2.013
4.590476.3
93,698.1
4.498 - 4.682
10.7 - 16.0
13.3 Pass
4.590491.3
28,333.0
4.498 - 4.682
3.2 - 4.8
4.0 Pass
*** Analyte #1: THC-Acid (deriv) Expected RT = 4.600
Rt (min)m/z
Area
Time Range
Ratio RangeRatio
P/F ng/mL
4.596371.2
1,855,395.7
4.504 - 4.688
100
100. Pass
4.038
4.596473.3
244,108.5
4.504 - 4.688
10.8 - 16.3
13.1
Pass
4.596488.3
82,158.2
4.504 - 4.688
3.5 - 5.3
4.4
Pass
Figure 6. TurboMass software provides standard and customizedreport templates.
PreVent modes maximize productivity
PreVent accessory
The PerkinElmer exclusive PreVent productivity tool kitoffers five modes of powerful operation for improvingchromatographic performance, minimizing system down-time and enhancing productivity. Only the PerkinElmerPreVent accessory offers all five modes of operation.
■ MSVent™ mode allows changing of columns withoutcooling and venting the Clarus 500 MS, reducinginstrument downtime, offering a significant timesavings. In addition, MSVent facilitates connection of the vent to a second detector for dual-signalcapability, providing greater flexibility and enhancing productivity.
■ Isolation mode eliminates downtime during routineinjector maintenance and can provide an extra 100analytical hours of productivity per system per year.
When running in Isolation mode, there is no need to interrupt the carrier-gas flow when disassemblingthe Clarus 500 GC injectors for routine maintenance(Figure 7), such as changing the glass liner. Time-consuming oven cool-down and vacuum shutdownperiods are eliminated.
■ Enhanced Large Volume Injection (ELVI) modefacilitates manual injection volumes of up to 150 µLand automatic injection volumes up to 50 µL withoutsolvent reaching the column or the detector, mini-mizing contamination. By eliminating solvents, large volume injection makes time-consuming andexpensive pre-concentration steps unnecessary,significantly decreasing the cost of analysis.
6
Feature Benefit
MSVent mode Eliminates downtime during removal and change of columns.
Allows connection of the vent to a second detector for enhanced flexibility and productivity.
Isolation mode Eliminates downtime during routine injector maintenance.
Provides approximately 100 extra analytical hours of productivity per system per year.
ELVI mode Facilitates manual injection volumes up to 150 µL and automatic injection volumes up
to 50 µL without solvent reaching the column or detector.
Offers unparalleled sensitivity and eliminates lengthy sample preparation.
Time-Saver mode Prevents unwanted, high molecular weight material from reaching the detector.
Shortens analysis time, facilitates isothermal chromatography and protects the detector
from contamination.
Allows changing of the analytical column without venting the mass spectrometer.
ProTect mode Eliminates contamination by preventing heavy components in the sample from reaching
the expensive and retentive chromatographic column.
Allows backflushing during a chromatographic run.
PreVent Accessory Benefits
■ Time-Saver mode prevents unwanted, high-boilingmaterial from reaching the detector, thus shorteninganalysis times and increasing throughput. It facilitatesisothermal chromatography and protects the detectorfrom costly and time-consuming contamination.
PreVent in Time-Saver mode allows changing of theanalytical column without venting the mass spectro-meter, a huge productivity gain for any lab.
■ ProTect™ mode makes many difficult separations ofvolatile components in semivolatile samples possible.In addition, this mode prevents heavy components in the sample from reaching the expensive and veryretentive chromatographic column, saving hours ofcleaning and disassembling procedures. ProTect alsoallows backflushing even during chromatographic runs.
Wide mass range meets complex analytical needs
Because of superior design quality, the Clarus 500 GC/MShas the widest mass range (1.0-1200 Da) available in its class. This gives the capability to analyze highermolecular weight compounds (Figure 8), such as highlyhalogenated compounds and drug derivatives. Even forroutine GC/MS analyses (< 800 Da), the Clarus 500 MSruns far from the quadrupole’s limit, ensuring uncom-promised performance over a wide mass range.
www.perkinelmer.com 7
Figure 7. Background signal on the Clarus 500 GC/MS while theinjector is being serviced. PreVent is operating in Isolation mode.
Figure 8. The Clarus 500 GC/MS 1200 Da mass range captures morecomplete sample information. Shaded area illustrates data missed by lower-mass-range analyzers.
AutoBuild method builder
The unique AutoBuild method builder automaticallysearches spectral libraries for chromatogram peaks andimports them into a quantification method. Names,retention times, spectra and quantification ions of therespective peaks are also imported. AutoBuild savestime building complex, multi-component methods.
rugged design for maximum uptime
The Clarus 500 GC/MS is rugged so it delivers reliability,reduced cost and easy maintenance for maximum uptime.The highly specialized gold component technologyenhances overall instrument stability and reducesmaintenance requirements by minimizing contamination.
The inner ion source is easily accessed from the frontof the instrument without removing the cover or ionoptics from the vacuum manifold. This allows a quicksource changeover in less than 15 minutes and reducesthe risk of contaminating the quadrupole analyzer.Because the Electron Ionization (EI) or ChemicalIonization (CI) sources have no wires, routine cleaningis simple. The filament system is rugged, performingconsistently analysis after analysis, with a self-aligningdesign that makes filament replacement quick and easy.
To maximize spectral quality, the instrument ion sourceand transfer-line temperatures are controlled independ-ently, reducing contamination and protecting thermallylabile compounds from uncontrolled fragmentation.The 350 °C upper temperature limit for the GC transferline and both EI and CI ion sources allow high-temper-ature operation for maximum cleanliness and perform-ance with high-boiling compounds. To simplify
maintenance, cleanable RF-only prefilters are located in front of the quadrupole analyzer.
The Clarus 500 GC/MS improves uptime and through-put of the laboratory with an air-cooled 250 L/secturbomolecular pump that allows up to 5 mL/minhelium carrier gas flow (EI mode). The pump-downtime is minimal (under 3 minutes), qualitative stabilityis reached in under 15 minutes and quantitative stabilityis reached in less than 90 minutes. A water-cooled pumpis optional.
The sealed, long-life photomultiplier detector lowers operating costs by eliminating expensive andcontamination-prone electron multipliers that needperiodic replacement. In the event carrier gas pressuredrops, the unique PerkinElmer Programmable PneumaticControl (PPC) pneumatics safeguard the system fromcolumn damage and ion-source contamination by auto-matically turning off the GC oven and transfer line heating.
Also, you will never need to worry about poweroutages because the Clarus 500 MS vent can beattached to a nitrogen purge gas stream. This prohibitsoxygen from entering the system during venting,minimizing contamination.
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Clarus 500 GC/MS Benefits
Feature Benefit
Fastest scan rates Provide unprecedented speed with better chromatographic peak definition,
ensuring better quality results.
Open the use of quadrupole technology to fast GC applications.
Widest mass range Offers uncompromised performance over a wide mass range.
SIFI scanning mode Includes Selected Ion Monitoring (SIM) and full scan in the same run,
resulting in the most complete characterization of the sample.
Eliminates the need to run the sample twice.
Gold component technology Improves stability and reduces maintenance.
Easy-access ion source Allows changeover in less than 15 minutes.
Sealed, long-life photomultiplier Eliminates expensive replacement costs of electron multipliers.
Improves reliability.
flexibility for customized analysis
Depending on your application, the Clarus 500 MS canuse either EI or CI sources in either positive or negativemode (Figure 9). EI produces high-quality classicalspectra that are the basis for compound identificationby library searching. The optional CI mode can be used for any of the following:
• if molecular weight information is needed
• for additional selectivity with complex samples
• when detailed characterization is required
You can run EI mode for rapid screening withoutchanging the CI source, a productivity benefit for your lab.
Further flexibility and creativity with analysis can beachieved through the extensive choice of acquisition-mode options. Full Scan mode is available with Centroidmode and Continuum mode running independently orsimultaneously, while Selected Ion Monitoring modecan run independently or concurrently with the variousFull Scan modes. Overall, there are 32 combinations ofacquisition modes available, giving the greatest amountof information from one sample.
More column choices
Because the Clarus 500 GC/MS allows a carrier-gasflow of up to 5 mL per minute in EI, you have morecolumn choices than with other GC/MS systems.Narrow-, medium- and wide-bore columns can be used, with almost unlimited choice of stationary phase, column diameter and analytical method with the Clarus 500 GC/MS.
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Internal Typical Clarus 500 Column Diameter Flows GC/MS Others
Narrow 0.10 to 0.1 to ✓ ✓bore 0.18 mm ID 0.8 mL/min
Medium 0.25 mm ID 1 to ✓ ✓bore 1.5 mL/min
Wide 0.32 to 3 to ✓bore 0.53 mm ID 5 mL/min
Figure 9. The three ionization modes of EI, CI+ and CI- can determinecomplete molecular information about your sample.
integrated system solutions
The PerkinElmer Headspace Sampler or ThermalDesorber, the industry-leading GC/MS sample handlingaccessories, provide powerful analytical capabilities foryour specific sample matrices.
Headspace Sampler with the Clarus 500 GC/MS
Toxic compounds in blood
Volatile chemicals, used as solvents, are present inpetrochemical derivatives, paint and a variety of otherindustrial products. These airborne compounds cancause severe and chronic cases of toxic exposure ofworkers in occupational areas. Headspace GC/MSanalysis is the ideal technique for specific and sensi-tive determination of these toxic compounds in bloodor urine. The use of a PerkinElmer TurboMatrix™
Headspace Sampler along with the fully automatedClarus 500 GC/MS allows analysis of large numbers of such samples.
TurboMatrix Headspace Sampler models with built-intrapping capability maximize the extraction and transferof headspace vapor into the Clarus 500 GC/MS, loweringdetection limits up to 100 times.
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Headspace Sampling with the Clarus 500 GC/MS
• Volatile organics in soils
• Volatile priority pollutants in water
• Petrochemical pollution in seafood
• Airborne toxic volatile compounds in human tissue and body fluids
• Off-odors and off-flavors due to printed ink solvents in food packaging films
• Residual solvents in pharmaceuticals
Thermal Desorption Systemwith the Clarus 500 GC/MS
Air toxics analysis
PerkinElmer has worked closely with the U.S. Environ-mental Protection Agency (EPA) and similar agenciesaround the world to develop instrumentation andmethodologies to measure Hazardous Air Pollutants(HAPs). The fully integrated Air Toxics Analyzeridentifies and quantifies volatile organic compounds in ambient air per U.S. EPA Method TO-17.
The Air Toxics Analyzer combines automated thermaldesorption sampling, gas-chromatographic separationand detection via mass spectrometry into a singlesystem. The PerkinElmer TurboMatrix Thermal Desorbersystem can process up to 50 sorbent tubes in a fullyautomated fashion.
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Thermal Desorption Sampling with the Clarus 500 GC/MS
• Air Toxics (U.S. EPA Method TO-17)
• Off-odors due to food packaging films
• VOCs in food/pharmaceutical packaging
• Forensics (arson accelerants)
• PCBs in soil
For a complete listing of our global offices, visit www.perkinelmer.com/lasoffices
©2004 PerkinElmer, Inc. All rights reserved. The PerkinElmer logo and design are registered trademarks of PerkinElmer, Inc. TurboMass, ProTect, SIFI and MSVent are trademarks,OneSource is a service mark and PerkinElmer and Clarus are registered trademarks of PerkinElmer, Inc. or its subsidiaries, in the United States and other countries. Visual Basicis a trademark of Microsoft Corporation. All other trademarks not owned by PerkinElmer, Inc. or its subsidiaries that are depicted herein are the property of their respectiveowners. PerkinElmer reserves the right to change this document at any time without notice and disclaims liability for editorial, pictorial or typographical errors.
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