UC Davis/NIH NeuroMab Facility Department of Neurobiology, Physiology and Behavior, UC Davis, Davis CA 95616-8519 http://neuromab.ucdavis.edu neuromab@ucdavis.edu (530) 752-8398

A cooperative venture among the University of California at Davis, the National Institutes of Health, and Antibodies Inc.

Anti-PSD-95 MAGUK scaffold protein, NeuroMab clone K28/43 Immunogen: Fusion protein amino acids 77-299 of human PSD-95/SAP90 (accession number P78352) Rat: 100% identity Mouse=99% identity (221/223 amino acids) No crossreactivity against recombinant Chapsyn-110, SAP97 or SAP102 Monoclonal antibody info: Mouse strain: Balb/C Myeloma cell: SP2/0 Mouse Ig Isotype: IgG2a NeuroMab Applications: Immunoblot, Immunohistochemistry, immuno-gold EM, and Immunoprecipitation Species Reactivity: human, mouse, rat MW: 95-110 kDa (varies with cell background due to phosphorylation) Top: Immunoblot versus adult rat brain membranes (RBM), and adult mouse hippocampal membranes (MHM) from PSD-95 knockout and WT mice (mouse samples courtesy of Dr. Richard Huganir) Middle: Electron micrograph shows K28/43 immunolabelling in hippocampus using a post-embedding immunogold method. Immunoparticles (arrows) were observed in the postsynaptic densities of dendritic spines (s) establishing asymmetrical synapses with axon terminals (b). Scale bar = 200 nm. Courtesy Dr. Rafael Lujan (Universidad de Castilla-La Mancha). Bottom: Cultured rat hippocampal neuron immunofluorescence K28/43 = green; K57/1 (anti-Kv4.2) = red