Upload
ingrid
View
22
Download
3
Tags:
Embed Size (px)
DESCRIPTION
Essential and toxic microelements in grains of Fusarium -infected maize and wheat genotypes: ICP-MS analysis. Zsuzsanna Kolbert, Attila Ördög, Ágnes Vashegyi, László Erdei. Two countries, one goal, joint success!. Materials and methods. Plant material : - PowerPoint PPT Presentation
Citation preview
Two countries, one goal, joint success!
Essential and toxic microelements in grains of Fusarium-infected maize and wheat genotypes:
ICP-MS analysis
Zsuzsanna Kolbert, Attila Ördög, Ágnes Vashegyi, László Erdei
Materials and methods
Two countries, one goal, joint success!
Sample preparation:•500 – 1000 mg from each sample•microwave digestion in nitric acid and hydrogen peroxide•dilution to 200 - 400 X
Measurement:•Microelement analysis (Mn, Fe, Co, Ni, Cu, Zn, As, Se, Mo, Cd, Pb) by ICP-MS device
Plant material:•2 maize genotypes (two sampling times)•13 wheat genotypes
Infection:•Fusarium graminearum•Fusarium culmorum•Fusarium verticillioides
The advantages of an ICP-MS
• More than 1000 samples in one week (up to 250 samples/day)• More than 15 000 data from the analysis • Lot of information in a few seconds (less than a second per
element)• User-friendly software (PlasmaLab), which makes the data
analysis easier and faster
Two countries, one goal, joint success!
Microelement contents in the two maize genotypes
Two countries, one goal, joint success!
GK-Sarolta GK-Boglár
Mn content in maize genotypes
Two countries, one goal, joint success!
Two countries, one goal, joint success!
Fe content in maize genotypes
Two countries, one goal, joint success!
Co content in maize genotypes
Two countries, one goal, joint success!
Ni content in maize genotypes
Two countries, one goal, joint success!
Cu content in maize genotypes
Two countries, one goal, joint success!
Zn content in maize genotypes
Two countries, one goal, joint success!
As content in maize genotypes
Two countries, one goal, joint success!
Se content in maize genotypes
Two countries, one goal, joint success!
Mo content in maize genotypes
Two countries, one goal, joint success!
Cd content in maize genotypes
Two countries, one goal, joint success!
Pb content in maize genotypes
Two countries, one goal, joint success!
Microelement contents in 13 different wheat genotypes
• Manastur - TM• Sanandrei - TM• Faget-TM• Pietroasa- TM• Lovrin -TM• Aldesti -TM• Dudestii Vechi -TM• Horea - Ar• Siria - Ar• Recas - Ar• Semlac - Ar• Paulis - Ar• Barzava - Ar
Mn content in different wheat genotypes
Two countries, one goal, joint success!
Fe content in different wheat genotypes
Two countries, one goal, joint success!
Co content in different wheat genotypes
Two countries, one goal, joint success!
Ni content in different wheat genotypes
Two countries, one goal, joint success!
Cu content in different wheat genotypes
Two countries, one goal, joint success!
Zn content in different wheat genotypes
Two countries, one goal, joint success!
As content in different wheat genotypes
Two countries, one goal, joint success!
Se content in different wheat genotypes
Two countries, one goal, joint success!
Two countries, one goal, joint success!
Mo content in different wheat genotypes
Two countries, one goal, joint success!
Cd content in different wheat genotypes
Two countries, one goal, joint success!
Pb content in different wheat genotypes
Two countries, one goal, joint success!
Pb content in different wheat genotypes
Summary
Maize genotypes:•Infections by all Fusarium strains increased the concentrations of microelements (Mn, Fe, Ni, Cu) and toxic metals (Cd, Pb) in the grains
Wheat genotypes:•Mn and Ni concentration were high in Barzava-Ar•Fe, Co, As content were higher in the chaff compared to grains in all genotypes•In the chaff of Horea-Ar high Pb and As concentrations were measured protection for the grain
Two countries, one goal, joint success!
Two countries, one goal, joint success!
THANK YOU FOR YOUR ATTENTION!