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Turbo-charged biofuel output: Moving beyond the easy sugars to access the
entire plant biomass
The only way to produce biofuel economically is to convert the entire biomass—not just the easy sugars—
of the targeted feedstock to biofuels
The Key to Success is Having the Right Microbe for the Job
Oleaginous microbes for the production of oil as a
feedstock for drop-in fuels
Advantages:• No molecular biology necessary
• Simultaneous growth optimization
• Rapid and robust selection
Experimental Evolution with BioTork Technology
Growth rate
Lipidproduction
Targettemperatures
Toxiccompounds
Carbonsources
Real world biomass
Successfully Evolved Traits
Maximizing energy yield from sweet sorghum
Why sweet sorghum?
• Can be planted in ⅔ of the US
• Tolerates poor soil, salt, dry climates
• 50% less water, nitrogen than corn
• Annual crop, two harvests per year
• Fast growing, short time to harvest
• Large quantities of both “easy sugars” and biomass
Juice•“Easy Sugars”: sucrose, glucose, fructose•Protein, other carbs
Bagasse Solubles•“Easy Sugars”: residual monosaccharides, starch•Protein, other carbs
Bagasse Insolubles•“Hard sugars”: cellulose, hemicellulose•Lignin
Carbon Sources in Sweet Sorghum
Evolved Oleaginous Microbes on Each Component
Juice
Bagasse Solubles/Insolubles
Corn Ethanol
Sweet Sorghum Ethanol
Sweet SorghumGreen Diesel(juice only)
Sweet SorghumGreen Diesel(w/bagasse)
Biofuel Yieldgal/acre 330-424 270-750 193-495 457-841
Energy Yield1000 MJ/acre 29-38 24-67 27-68 64-118
IncrementalValue$/acre
0 (84)-456 117-891 909-1929
Yield and Revenue Potential
• Highest yield for same input feedstock
• Simple fermentation technology
• Reduced waste
• Distributed biorefineries using local feedstock
• Rural development
With BioTork Technology: The right microbe for the job
Opening the door to other agricultural by-products:
Future Developments With BioTork Technology:
• Agricultural residues (stover, straw)• Biodiesel-derived waste glycerol• Woody biomass• Seed cake• Stillage• Fruit culls
Thank You…..