tsn1ts

n2

tsn1

tsn2

WT

TSN2

TSN1

28S

28S

A

B

ColC

3 kb

3 kb

RB LB

RB LB LB RB

tsn2

TSN1

TSN2

Figure 1

TSN

NMT

110kD77kD

D

tsn1

tsn1ts

n2

SN SN SN SN TUDOR SN

A

Roo

t len

gth

(cm

)

Col

tsn1

tsn1

tsn2

TSN

1EC

tsn2 Ws

tsn1

tsn2

TSN

2EC

* ** **

Figure 2.

TSN

NMT

B

**

Figure 3

TSN1

DiSR pics here ( keep GUS for DiSR paper)

TSN

Comassie

Roo

ts (1

0 da

g)

Leav

es (1

0 da

g)

Ros

ette

leav

es (2

1 da

g)

Flow

er b

uds

Ope

n flo

wer

s

Gre

en s

iliqu

es (

5daf

)

Dry

see

ds

A

B

TSN2

rela

tive

fresh

wei

ght (

%)

Col

tsn1

tsn1t

sn2

TSN1

EC

tsn2

Ws

TSN2

EC

B

* *A

Figure 4.

100mM NaCl

TSN1ECtsn1tsn2

tsn1t

sn2

14/28 3/24

2 : 0,009 dans fig legend

*

* *

*150 mM NaCl

Ger

min

atio

n %

Ger

min

atio

n %

ABA (µM)

NaCl (mM)

ABA (µM)

NaCl (mM)

A

****

****

****

(tsn1) *

(tsn2) ** ****

****

Figure 5

C

Coltsn2Wstsn1

TSN1EC

tsn1tsn2TSN2EC

tsn1tsn2

B

DTSN1EC

tsn1tsn2TSN2EC

tsn1tsn2

Coltsn2Wstsn1

Ger

min

atio

n %

Ger

min

atio

n %

0 0.1 1 10 0 0.1 1 10

Figure 6

Col

tsn1

tsn2

Ws

See

d se

t (m

g)

* **

TSN1E

C

TSN2E

C

tsn1ts

n2

tsn1ts

n2

**

Figure 7 light ( copie d’écran)

Predicted signal peptide

Other

Reference mRNA poolN=3468

mRNA downregulated in tsn1tsn2tsn1tsn2 / TSN1EC, N=53

(* Pval < 0.05)

Figure 8

C

tsn1tsn2 / TSN1EC

tsn1tsn2 / TSN2EC

37

16

16

12

tsn1tsn2 / TSN1EC

tsn1tsn2 / TSN2EC

A B

=23

=17

=53

=32 611

Downregulated Upregulated

D17%

83%75%

25%

E

a.u.

a.u.

- T D – T D – T D

- T D – T D – T D

- T D – T D – T D

- T D – T D – T D

At1g54000 At5g28540

At4g29520 At5g42020

AGI Valid TAIR SP CRPDownregulated P r Av. sd

AT3G53980 0,0E+00 -4,3 -4,3 0,8 + protease inhibitor/seed storage/lipid transfer protein (LTP) + CRP4730 AT4G27080 1 0,0E+00 -3,7 -1,2 0,3 - thioredoxin family protein - n.a.

AT2G43510 0,0E+00 -3,6 -3,8 2,1 + trypsin inhibitor, putative + CRP0700AT2G19970 0,0E+00 -3,6 -3,2 0,9 + pathogenesis-related protein, putative + n.a.AT3G58550 0,0E+00 -2,9 -3,2 0,1 + protease inhibitor/seed storage/lipid transfer protein (LTP) + CRP4660AT3G22840 0,0E+00 -2,6 -1,3 0,1 - chlorophyll A-B binding family protein / early light-induced protein (ELIP) - n.a.AT3G04720 0,0E+00 -2,5 -2,8 0,6 + hevein-like protein (HEL) / PR4 + CRP5800AT3G08770 0,0E+00 -2,3 -2,9 1,3 + lipid transfer protein 6 (LTP6) + CRP3860AT2G18370 0,0E+00 -2,3 -2,4 0,4 + protease inhibitor/seed storage/lipid transfer protein (LTP) + CRP4060

AT2G16005 2 0,0E+00 -2,0 -3 1,4 + MD-2-related lipid recognition domain-containing protein + n.a. AT1G73330 2 0,0E+00 -1,7 -2,1 0,2 + protease inhibitor, putative (DR4) + CRP6080Upregulated

AT5G63160 0,0E+00 2,3 1,0 0,7 - speckle-type POZ protein-related - n.a.AT1G19960 0,0E+00 2,3 1,2 0,6 - expressed protein - n.a.AT1G15380 5,8E-12 2,3 0,7 0,8 - lactoylglutathione lyase family protein - n.a.AT4G13770 1,9E-09 2,1 2,9 1,0 + cytochrome P450 family protein (CYP83A1) - n.a.

AT5G23020 2 2,1E-06 1,9 n.a. n.a. ? 2-isopropylmalate synthase 2 (IMS2/MAM3) - n.a.

qPCRChip

Table 1