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Figure S7. SlSERK3A and SlSER3B transcript levels and weight in silenced roots. (A) Transcript levels of VIGS-silenced genes were evaluated using qRT-PCR. Tomato cv. Moneymaker plants treated with TRV empty vector (TRV), TRV-SlSERK3A, TRV-SlSERK3B were evaluated. Expression was normalized against UBI3. A subsample from six different roots was analyzed per construct. Values are average + SE of three technical replicates. *P < 0.05 significant difference from TRV (two- sample t-test). (B) Root weight of RKN infected plants. Results are average (±) SE (n=9) shows no significance difference (ANOVA Tukey HD test).This experiment was repeated once with similar results. TRV TRV-SERK3A TRV-SERK3B 0 10 20 30 40 50 60 70 Weight per root (g) TRV SER K3 A-1 SER K3A-2 SER K3A-3 SER K3A-4 SERK3A-5 SERK3 A-6 SERK3B-1 SER K3 B-2 SER K3B-3 SER K3 B-4 SER K3B-5 SER K3 B-6 0 0.2 0.4 0.6 0.8 1 1.2 1.4 SlSERK3A SlSERK3B Relative amount TRV TRV-SlSERK3A-1 TRV- SlSERK3A-2 TRV- SlSERK3A-3 TRV-SlSERK3B -1 TRV-SlSERK3B-2 TRV-SlSERK3B -3 * TRV-SlSERK3B -4 TRV-SlSERK3A-4 * * * * * TRV B A * * * * TRV-SlSERK3B-5 TRV-SlSERK3B -6 TRV-SlSERK3A-5 TRV-SlSERK3A-6 TRV-SlSERK3B TRV- SlSERK3A

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A. *. *. *. *. *. *. *. *. *. *. TRV. TRV- SlSERK3A -1. TRV- SlSERK3A -2. TRV- SlSERK3B -2. TRV- SlSERK3A -5. TRV- SlSERK3A -6. TRV- SlSERK3B -3. TRV- SlSERK3A -3. TRV- SlSERK3B -1. TRV- SlSERK3B -6. TRV- SlSERK3A -4. TRV- SlSERK3B -5. TRV- SlSERK3B -4. B. TRV. - PowerPoint PPT Presentation

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Page 1: TRV

Figure S7. SlSERK3A and SlSER3B transcript levels and weight in silenced roots. (A) Transcript levels of VIGS-silenced genes were evaluated using qRT-PCR. Tomato cv. Moneymaker plants treated with TRV empty vector (TRV), TRV-SlSERK3A, TRV-SlSERK3B were evaluated. Expression was normalized against UBI3. A subsample from six different roots was analyzed per construct. Values are average + SE of three technical replicates. *P < 0.05 significant difference from TRV (two-sample t-test). (B) Root weight of RKN infected plants. Results are average (±) SE (n=9) shows no significance difference (ANOVA Tukey HD test).This experiment was repeated once with similar results.

TRV TRV-SERK3A TRV-SERK3B0

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