TruTOF HT® High Throughput TOFMS

®

Delivering the Right Results

LECO’s TruTOF HT TOFMS®

High Throughput Time-of-Flight Mass Spectrometer

The TruTOF HT TOFMS combines LECO’s benchtop Time-of-Flight Mass Spectrometer (TOFMS) withChromaTOF software to provide revolutionary Time-Compressed Chromatography, Automated Peak Find,and True Signal Deconvolution in a high throughput, benchtop GCMS. No other benchtop system canmatch the TruTOF, with the reliability you expect from LECO.

®

®

speed and accuracy of

ChromaTOF Workstation• Windows -based platform®

• High-speed processor

• Large data storage capacity

• Remote diagnostics

TruTOF HT TOFMS• Fast spectral acquisition rate (80 Hz)

• Electron ionization (EI) andchemical ionization (CI) sources

• Reliability you expect from LECO

The solution to your analytical challenges.

Patents include no. US4490806, US5175430, US5367162,IT1167562, WO9220435,

EP540720, CA1225155. Other patents pending.US5712480, WO8304326, WO9500236,

The Power of LECO TOFMSFeatures BenefitsBenchtop design Affordable GC-TOF capabilities • High throughput in a space-saving package

Full-range mass sensitivity superior to SIM

Isolated Ion Source (Gate Valve) Plug & play ion source for faster source swapping, automatic source identification and improved productivity

Full-range mass sensitivity 2 pg of Hexachlorobenzene on column produces signal-to-noise ratio >10 at mass 284 (EI)

10 pg of Benzophenone on column produces signal-to-noise ratio >10 at mass 183 (CI)

Accomplish target and unknown analyses in one run

Linear dynamic range of4 orders of magnitude or more

Analyze and quantitate real-life samples of varying concentrations in matrix, reducing the need fortime-consuming dilutions & re-analysis

Electron (EI) and Chemical(CI) Ionization sources

Offering you the option between comparison with classic NIST spectra (EI) and superiorpreservation of the molecular ion (CI)

ChromaTOF software

Integrated control fromChromaTOF software

EPA Reporting Software

Automated Peak Find and True Signal Deconvolution for fast/easy data analysis and clean library searchesCustom report generationComparison feature for fast and easy sample analysis and the ability to quickly determine quality lots

GC unit • Multiple sample-handling accessories available including LEAP, Gerstel, and AgilentRemote start, contact closures for other instrumentation • Total system automation for maximum productivity

CLP-style reports • Fully customizable • Fully integrated within ChromaTOF

High Throughput GCMSHigh throughput is the key to increasedprofitability and faster results. The need forSIM-mode operation and low dynamic rangeassociated with traditional quadrupoles andion traps take precious time and money awayfrom your laboratory’s bottom-line. TheTruTOF HT combines a fast acquisition massspectrometer (80 Hz) with patented data-mining algorithms to produce the speed andresolution necessary to accomplish Time-Compressed Chromatography at anaffordable base price.

• Greater sample throughput byreducing run times

• Less sample degradation

• Faster method development

• No statistical under-sampling(associated with scanning detectors)

• Automated Peak Finding andTrue Signal Deconvolution

Sampling

RoughingPump

SourceChamber

TransferLine

Detector

AnalyzerChamber

VacuumGauge

OrthogonalAccelerator

GasFilter

Reflectron

PowerElectronicsand Control

GateValve

Integrated TransientRecorder

Trigger Out

TurboMolecularPumps

CarrierGas Flow

GasChromatograph

IonSource

TruTOF HT Diagram

World’s Fastest Benchtop Mass SpectrometerUp to 80 full-range mass spectra/second (80 Hz)It is well agreed upon in the scientific community that a minimum of 10 data points across a fully-resolvedchromatographic peak are needed for proper peak definition. However, 18-20 points are required for Automated PeakFind, True Signal Deconvolution, and proper peak identification and quantification in complex samples. Given that theTruTOF TOFMS detector is the only MS detector in its price range capable of collecting more than 50 full-range massspectra/second; it is the ideal detector for . With user-defined collectionrates up to 80 Hz, the TruTOF TOFMS can deliver sufficient data density to accurately characterize GC peaks andincrease productivity.

Routine GC Analysis and High-Speed GC

An inherent advantage of TOFMS with time-arraydetection is the complete absence of ion con-centration biasing (spectral skewing). Withscanning instruments, the concentration in thechromatographic peak, and thus in the MS ionsource, changes for each mass during a scan. Ifthe concentration change is significant, spectraldeconvolution of unknown peaks with significantchromatographic coelution is unreliable. TOFMSovercomes this limitation and provides spectra thatare free of skewing across the entire peak.

TOFMS GC Peak Scanning MS

1 spectra/secondNo peaks identified

20 spectra/second8 peaks identified

40 spectra/secondAll 9 peaks identified

In this example, the ability to acquire data greater than 20 spectra/second (20 Hz) is necessary for automated peakidentification and deconvolution of all 9 pesticides in this mixture.

Acquisition Speed without Spectral Continuity=Limited DeconvolutionSpectral Continuity without Acquisition Speed=Limited Deconvolution

Acquisition Speed and Spectral Continuity=True Signal Deconvolution!

Spectral Continuity

Effect of Acquisition Speed on Automated Peak Find

1000

Rel.

Abu

nd. 750

500

250

0060 80 100 120 140

m/z

1000

Rel.

Abu

nd. 750

500

250

060 80 100 120 140

m/z

m/z

1000

Rel.

Abu

nd. 750

500

250

060 80 100 120 140

m/z

1000

Rel.

Abu

nd. 750

500

250

060 80 100 120 140

m/z

1000

Rel.

Abu

nd. 750

500

250

060 80 100 120 140

m/z

1000

Rel.

Abu

nd. 750

500

250

060 80 100 120 140

Inte

nsity

Time (sec)

8000

6000

4000

2000

012.5 13.0 13.5

Inte

nsity

Time (sec)

8000

6000

4000

2000

012.5 13.0 13.5

Inte

nsity

Time (sec)

8000

6000

4000

2000

012.5 13.0 13.5

Time (seconds) 157 158 159 160 161 Time (seconds) 157 158 159 160 161

Met

olac

hlor

Mal

athi

on

Fent

hion

Cho

rpyr

ifos

DC

PA

Cya

nazi

ne

Isod

rin

Tric

hlor

onat

Time (seconds) 157 158 159 160 161

Met

olac

hlor

Mal

athi

on

Fent

hion

Cho

rpyr

ifos

Para

thio

n

DC

PA

Cya

nazi

ne

Isod

rin

Tric

hlor

onat

Acquisition Speed and Spectral Continuity=True Signal Deconvolution!

Automated Peak Find and True Signal Deconvolution®

Detecting low-level unknown analytes is an extremelydifficult task. Typically, the analyst is left to search the TotalIon Current Chromatogram (TIC), looking for smalldeviations in the baseline. The more complex the samplematrix the greater the background signal encountered inthe TIC, causing the unknown analytes to be quickly lostin the background.

8 peaks are observed in this 13-second interval of the TIC.

Seconds 1 3 5 7 9 11 13

The Peak Find algorithm automatically locates all peaks inthe chromatogram when provided with only an expectedchromatographic peak width and a signal-to-noisethreshhold. Coeluting analytes, peaks buried beneath thebackground of the TIC, and even small peaks buriedbeneath large matrix interferences are automaticallydetected and reported.

Seconds 1 3 5 7 9 11 13

The Peak Find algorithm automatically detects 21 analytesin the same 13-second section of the chromatogram.

Automated Peak Find

True Signal Deconvolution (TSD)

0

Shown for peaks 56 and 58 are the caliper (A), deconvoluted Peak True (B), and corresponding library spectra (C). True Signal Deconvolutionautomatically removes background signal from the caliper spectrum resulting in the deconvoluted Peak True spectrum.The Peak True spectrum is then used for peak identification by automatically comparing against standard libraries (commercially-availableor user-developed). In this spearmint oil sample, deconvolution has allowed for the identification of peaks 56 and 58 though they coelutewith other analytes in the sample. Notice the removal of shared signal in the caliper spectra (marked in red).

79.5 80 80.5 81 81.5 82 82.5 83Time (s)

56 58

121 117 71 57 TIC

50 100 150 200

121937753 154

50 100 150 200

132

9165

50 100 150 200

1000132

9164

Library Hit - similarity 887, "Styrene, 2,6-dimethyl”

117

117

50 100 150 200

121937753 154

Caliper

50 100 150 200

132

Peak True

50 100 150 200

1000132

9164

117

117

50 100 150 200

43 71 93121

154

50 100 150 200

57 85

139

50 100 150 200

43103 144

Library Hit - similarity 821, "2-Methylbutyl 2-methylbut

anoate"

57 85

50 100 150 200

43 71 93121

154

Caliper

50 100 150 200

57 85

139

Peak True

50 100 150 200

43103 144

57 85

A

TIC

A

B B

C C

True Signal Deconvolution (TSD )® ®

After the Automated Peak Find algorithm has located all of the analytes, a mass spectrum for each analyte isautomatically extracted free of interferences from the system background, matrix background, and coeluting analytesusing True Signal Deconvolution. Even ions that are shared between coeluting analytes are accurately proportioned toprovide you with a complete mass spectrum. The resulting spectrum is then used for analyte identification by spectralinterpretation or by library search using any number of commercially available or operator-prepared databases.

171 171.5 172 172.5 173 173.5 174Time (s)

TIC 57 41 107 122

50 100 150 200

1000107

775127

Library Hit - similarity 936, "Phenol, 2,6-dimethyl-"

50 100 150 200

1000122

7739

Peak True

107

122

50 100 150 200

1000122

77

Caliper

57

107

41

50 100 150 200

10005741

69 95

Peak True

50 100 150 200

10005741

70 98

Library Hit - similarity 902, "Nonanal"

50 100 150 200

10005741

69 95

Caliper

122107

5 6

177.5 178 178.5 179 179.5 180Time (s)

TIC 69 83 105 123 151

60 80 100 120 140 160 180 200

500

100069

83

1255717197

Peak True

143

60 80 100 120 140 160 180 200

500

100069

83

5717197

Caliper

143123

60 80 100 120 140 160 180 200

500

1000

151105

Peak True

123

60 80 100 120 140 160 180 200

500

1000

151105

Caliper

123

83695 6

Within the 3-second time window of the above chromatograms, the benefit of True Signal Deconvolution becomes apparent. The samesample analyzed under both EI and CI conditions produced a coelution between peaks 5 and 6. The top chromatogram shows the coelutionunder EI conditions with the unique ions for each component clearly visible in the caliper spectra (A). The Peak True spectra (B) for bothcomponents clearly shows the removal of the coeluting compound’s unique ions (in red) after deconvolution and the resulting match withthe NIST library (C). The bottom chromatogram shows the coelution under CI conditions. Again the unique ions of the coeluting compoundhave been removed by deconvolution as shown in the Peak True spectra (D). Additionally, CI has allowed for the visibility of the protonatedmolecular ions (in blue).

Insufficient dynamic range causes productive laboratoriesto lose their edge by requiring multiple analyses on thesame sample. A wide dynamic range is required to achievequantitative results on samples with widely varyingcompound concentration levels. The TruTOF HT preventsthat loss of productivity by offering a linear dynamic rangeof 4 orders of magnitude or more—decreasing the need fordilution and re-analysis of high concentration levelsamples, and increasing the throughput of your laboratory.The figure to the left shows a linear dynamic range of4 orders of magnitude for HCB (5 pg/uL to 50,000 pg/uL).

A A

B B

C C

D D

E E

EI

CI

Peak 5 Peak 6

Peak 5 Peak 6

Dynamic Range

10000 20000 30000 40000 50000 60000 70000

5000000

10000000

15000000

20000000

25000000

30000000

35000000

40000000

45000000

Concentration (pg/µL)

Peak

Are

a

5,000 pg/µL

10,000 pg/µL

20,000 pg/µL

30,000 pg/µL

50,000 pg/µL

y= +936.582x + 1866.48

r = 0.99974

200 400 600 800 1000

200000

400000

600000

800000

1000000

1,000 pg/µL

500 pg/µL

100 pg/µL10 pg/µL

5 pg/µL

Productivity Optimization

• , , and

• for the reporting of non-calibrated compounds

• Automatic tuning within user-defined Quality Control methods

• Integrated control of a variety of sample handling options fromGerstel, LEAP, and Agilent

• Customizable user interface

• Automatically export data to PDF, CSV, ANDI MS, NETCDF, or Raw file formats

True Signal Deconvolution Automated Peak FindExtended Range Calibration

Semi-Quantitative Analysis

algorithms

• Fully-integrated to meet EPA requirementsEnvironmental Report Designer

ChromaTOF SoftwareChromaTOF software was designed with your laboratory in mind. With outstandingease-of-use, ChromaTOF encapsulates the industry’s most advanced qualitative andquantitative capabilities into one easy-to-use, seamless data-handling system.

ChromaTOF Software®

Form No. 209-187 R2.52-REV2 © 2010 LECO Corporation

LECO Corporation3000 Lakeview Avenue • St. Joseph, MI 49085 • Phone: 800-292-6141 • Fax: 269-982-8977

info@leco.com • www.leco.com • ISO-9001:2000 • No. FM 24045 • LECO is a registered trademark of LECO Corporation.

®

Delivering the Right Results

Life Sciences and Chemical Analysis Solutions

Every day around the world, LECO instruments continuously perform analyses for today's most complex applications.Whether you are analyzing samples in the food, flavor/fragrance, petroleum, environmental, or biotechnology(metabolomics) industries, we have an instrument configuration to meet your needs.

A Commitment to Quality and ServiceLECO instruments are noted for superior precision, speed, and ease-of-use. We are an international company with over25 subsidiaries worldwide. Our global network of sales/support is dedicated to customer service and satisfaction, and ourcommitment to quality is further underscored with ISO-9001:2000 certification. We conform to CE quality and safetyspecifications, fully testing our instruments at our on-site Compliance Testing Center.

Technical Research Center Global Support Center Life Science and Chemical Analysis Centre

GCxGC• Offers you enhanced separating power for complex sample analysis

• Easy-to-use ChromaTOF software

• Classification software feature simplifies component identification

• Flame Ionization Detector (FID) and Electron Capture Detector (ECD)

Pegasus 4D GCxGC-TOFMS®

• Pegasus, with a maximum acquisition rate of 500 spectra/second, offers you the onlyMS detector capable of comprehensive two-dimensional GC (GCxGC)

• ChromaTOF software gives you the ability to take your sample characterizationto the next level

• The ultimate in chromatographic resolution from the pioneer of GCxGC technology

Pegasus HT TOFMS• Acquisition speed of up to 500 spectra/second offers you the ideal MS detector for

unparalleled throughput

• Powerful ChromaTOF software simplifies componentidentification—providing a significant increase in efficiency and productivity

• Key features include automated data mining, chromatogram locking,reverse-library search mode, and data-dependent, user-defined QC method development

• Ability to upgrade to Pegasus 4D GCxGC

• Robust, never-clean ion source