Trichoderma resource collection, identification and

superior individual screening from marine shoal and

wetland ecosystem

Item number: 2014FY120900

Chengyun Li

Biological Institute of Shandong Academy of Sciences

9-9-2014

Introduction of Biological institute of

Shandong Academy of sciences

Beijing

Jinan

LOCATION

分院

Qian Fuo Shan Park

Biological institute of

Shandong Academy of

Sciences

BIOLOGICAL INSTITUTE

Biotechnology building

STAFF

Institute founded in 1983.

In total 104 research scientists and staff, including more than 40

senior scientists.

RESEARCH GROUPS

Key laboratory for biosensors of Shandong

province

Key laboratory for applied microbiology of

Shandong province (KLAMSP)

• Including applied microbiology,

biochemical engineering, seafood

research and drug screening.

Biopesticide, bio-fertilizer

Enzyme preparation (phytase ，glucanase)

Bioenergy microbial（Trichoderma, microalgae）

peppermint microalgae peppermint oil

bio-fertilizer

Application

KLAMSP

Biocontrol strains

KLAMSP

Gliocladium

Lysobacter Pseudomonas

Nematophagous Fungi

Bacillus

Trichoderma

Cotton Verticillium

Wilt

Vegetable Root-knot Nematodes

Wheat Sharp Eyespot

Rice Blast

Tomato Bacterial Wilt

Cucurbits Powdery Mildew

Plant Diseases Biocontrol

KLAMSP

Plant Diseases Biocontrol

Biopesticide products

KLAMSP

Until June 2014, 302 samples have been collected from 51 sites.

Bohai Sea

Yellow Sea

Current sample sites

Tools

Field work

Dongying-offshore area Dongying Tangshan

Tangshan Tianjin Tianjin

Huanghua Huanghua Tianjin

Lianyunguang Lianyunguang Xinqi

Yellow River Delta wetland Yellow River Delta wetland Yellow River Delta wetland

Haiyang Rongcheng Weifang

Trichoderma screening

Projects in process…..

Sampling time

I

II

III IV

V

2014: June, July, September October 2015: April, May

2014: June, July, 2015: September, October 2016: April, May

2014: October，November 2015: April, May 2016: June, July

2014: November, December 2015: April, May 2016: June, July

2015: September, October 2016: April, May

Isolation and Identification

1.Phenotype：Conidiophore (Gams & Bissett (1998))

2.Molecular characterization:

rRNA-ITS1，ITS2 sequence and Translation Elongation Factor tef1-α，phytase gene

phyT, and construction of Phylogenetic tree based on ITS and Tef1-a sequence;

3. DGGE and LAMP

1. Isolation and identification of 700 Trichoderma strains from

marine shoal and wetland ecosystem in whole China.

2. Isolation of ~200 strains from those above:

antagonistic

stress tolerance (salt, low T and hypoxia)

plant growth promoting

other particular properties.

Preliminary results

Mycoparasitism

Phytase-degrading

Trichoderma harzianum salt tolerance ability

Conclusions

• Trichoderma marine shoal and wetland

distribution map in China will be produced.

• Biodiversity of Trichoderma in extreme

environments (salt, low T and hypoxia) is

being studied.

Acknowledgment

• NSFC (National Science Foundation of China)

• Shanghai Jiao Tong University.

• Workshop Organizers (Dr. Ma, Dr. Wu, Ms Jing)