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Transfer of the F factor
Transfer of the Hfr F+ factor
Excision of the F+ factor also occurs
spontaneously at low frequency.
Begin with Hfr cell containing F+.
Small section of host chromosome also
may be excised, creating an F’
plasmid.
F’ plasmid is named for the gene it
carries, e.g., F’ (lac)
Interrupted
mating
experiment
F- bacteria
2 Conjugation F plasmid
Mating Bridge
F+ bacteria
F plasmid
2 Conjugation
F- bacteria
F plasmid
F+ bacteria
F plasmid
Mating Bridge
Genetic map-results of interrupted E. coli mating experiment
F+ factor (fertility factor)- a sophisticated plasmid
OriT, Origin of transfer
insertion sequences,
genes for own replication,
genes for own transfer
Conjugation converts an F- to F+. Original F+ remains F+
Note: No host genes (in blue) transferred from F+ to F-
To transfer host genes, need an Hfr strain
(high frequency)
Homologous
Recombination
here
One cell will have one or another of these
F+ to Hfr relative low frequency (~few %).
X-ray induces Hfrs. Why?,
Using an Hfr for gene transfer