Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus? DO NOW: (5 MINUTES) NOTE:

Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus?

DO NOW: (5 MINUTES) NOTE: READ AND ANSWER THE QUESTIONS BELOW.

Botana curus is a valuable plant because it produces CUROL, a substance used to treat certain type of cancer. Curol is not produced in the laboratory. Botana curus rows very slowly and is on the endangered species List. Species that are most closely related to Botana curus might produce Curol. These 3 similar plant species are SPECIES X, SPECIES Y andSPECIES Z. One of them is closely related to Botana curus and could able to produce curol.

QUESTIONS: 1. Why is Botana curus considered as a valuable plant?2. What is curol?3. What are the problems about Botana curus

AGENDA 03/04/09 DO NOW: • Silent ReadingMINI LESSON:• Gathering structural Evidences to prove relationships• Formulating hypothesis ACTIVITY: • Observing structural characteristics of Botana curus and species XYZ REFLECTIONHOMEWORKCastle Learning

Botana curus is a valuable plant because it produces Curol, a compound used for treating certain kinds of cancer. Curol can not be produced in the laboratory.

Botana curus grows very slowly and is on the endangered species list, so its ability to provide curol in large quantities is limited.

Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus? AGENDA 12/18/0 DO NOW: • Silent ReadingMINI LESSON:• Gathering structural Evidences to prove relationships• Formulating hypothesis ACTIVITY: • Observing structural characteristics of Botana curus and species XYZ REFLECTIONHOMEWORKCastle Learning

MINI LESSON: (10 to 15 mins ) AGENDA 03/04/09 DO NOW: • Silent ReadingMINI LESSON:• Gathering structural Evidences to prove relationships• Formulating hypothesis ACTIVITY: • Observing structural characteristics of Botana curus and species XYZ

REFLECTIONHOMEWORK

Castle Learning


AGENDA 03/04/09 DO NOW: • Silent ReadingMINI LESSON:• Gathering structural Evidences to prove relationships• Formulating hypothesis ACTIVITY: • Observing structural characteristics of Botana curus and species XYZ

REFLECTIONHOMEWORK

Castle Learning

TABLE 1: STRUCTURAL EVIDENCES SPECIES Structural

Characteristics of Plant(color of leaf&petals)

Structural Characteristics of Seeds(color and shape of seeds,)

MicroscopicStem Structure(scattered or circular)

BOTANA CURUS

SPECIES X

SPECIES Y

SPECIES Z



REFLECTIONHOMEWORK

Castle Learning


Structural Characteristic of Plants AGENDA 03/04/09 DO NOW: • Silent ReadingMINI LESSON:• Gathering structural Evidences to prove relationships• Formulating hypothesis ACTIVITY: • Observing structural characteristics of Botana curus and species XYZ

REFLECTIONHOMEWORK

Castle Learning


Structural Characteristic of Seeds AGENDA 03/04/09 DO NOW: • Silent ReadingMINI LESSON:• Gathering structural Evidences to prove relationships• Formulating hypothesis ACTIVITY: • Observing structural characteristics of Botana curus and species XYZ

REFLECTIONHOMEWORK

Castle Learning

Compare Stem Structures

Botana curus

Species X

Species Y Species Z



Microscopic Stem Structure- SCATERRED OR CIRCULAR?


REFLECTIONHOMEWORK

Castle Learning

http://images.google.com/imgres?imgurl=http://www.skidmore.edu/academics/biology/plant_bio/Anatomy-stems,seeds,embryos/Dicot%2520stem-x.s..jpg&imgrefurl=http://www.skidmore.edu/academics/biology/plant_bio/lab12ANATOMY.html&h=403&w=365&sz=58&tbnid=FlepnbMXfyoJ:&tbnh=121&tbnw=109&hl=en&start=1&prev=/images%3Fq%3DDicot%2BStem%26hl%3Den%26lr%3D%26ie%3DUTF-8%26safe%3Doff

http://images.google.com/imgres?imgurl=http://www.mun.ca/biology/singleton/Topic%252011/35-18a-DicotStem.jpg&imgrefurl=http://www.mun.ca/biology/singleton/Topic%252011/kStructure%26GrowthofFloweringPlants.htm&h=530&w=570&sz=86&tbnid=ZM0ChyzGHKkJ:&tbnh=121&tbnw=131&hl=en&start=7&prev=/images%3Fq%3DDicot%2BStem%26hl%3Den%26lr%3D%26ie%3DUTF-8%26safe%3Doff

HYPOTHESIZE: TESTS 1-3

A. Base on your data for structural relationships, which species ( X,Y,Z) would you hypothesize is most likely to produce CUROL? __________

B. Explain how the evidence from your data table supports your hypothesis.



Formulating Hypothesis AGENDA 03/04/09 DO NOW: • Silent ReadingMINI LESSON:• Gathering structural Evidences to prove relationships• Formulating hypothesis ACTIVITY: • Observing structural characteristics of Botana curus and species XYZ

REFLECTIONHOMEWORK

Castle Learning

TABLE 1: MOLECULAR EVIDENCES

SPECIES Paper Chromatography(color)

Test for ENZYME M (present / absent)

Differences in Amino Acid Sequences

Gel Electrophoresis Banding Pattern

B C

SPECIES X

SPECIES Y

SPECIES Z



REFLECTIONHOMEWORK

Castle Learning

TEST # 4: Paper Chromatography



REFLECTIONHOMEWORK

Castle Learning

Testing for “M”

Record the results of your tests for enzyme “M”

(either a Positive or Negative result) in Table 1

Botana curis

Species X

Species Y

Species Z


Base Pair Rule


DNA : GCAT GAA GGA CTCCTT CCT GAG

RNA : GCAU CAC GTG GTA

GUG CAC CAU

Translating DNA Code to Make Proteins

BOTANA CURUS CAC GTG GAC TGA GGA CTC CTC

Sequence of bases in mRna Produced ______________________________

Sequence of amino Acids in the protein _______________________________

GUGCAC CUG ACU CCU GAG GAG

VAL HIS LEU THR PRO GLU GLU



Gel Electrophoresis


Separation of DNA Fragments

GEL ELECTROHORESIS BANDING PATTERN

BC: ATTCCGGATCGATCGCCGGATATACTCCGGTTATATC

5- 12- 11- 9

X : ATTGTACCGGGATCCGGACGTCGCGACTAATATAGCA

8- 7- 22 Y : ACCGGTCCGGGATCGCACCCGGTACTCCTGTAATATC

3 5 12- 17 Z : ATTCCGGATCGATCGCCGGATATACTCCGGTTATATC

5- 12- 11- 9

Documents

Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus? DO NOW: (5 MINUTES) NOTE: