TOPIC: MicroscopeAIM: What are the parts of a microscope?

Microscope • Instrument that produces a larger image of an object (specimen) using lenses

Lens•Piece of curved glass • Causes light rays to come together or spread apart

•Magnify an image

SimpleMicroscope

•Uses 1 lens and light to magnify the specimen

CompoundLightMicroscope

•Uses 2 lenses & light to magnify specimen

•Need stain (dye) to see it

Blood

Cheek Cells 40x

Onion Cells 10x

PARTS OF A MICROSCOPE

1. eyepiece

•Ocular lens (usually 10x)

•Lens you look through

2. Objective

lenses

•Contains lenses •Low, medium, high power

Low Power

High Power

•Low power objective = used to locate the specimen on the slide

•Larger field of view (See more of the entire object)

•High power objective =•More magnification•Smaller field of view(see less

of the entire object)

3.Nosepiece

•Holds & rotates obj. lenses

4. Body Tube

•Connects eyepiece to nosepiece

5. Arm•Supports body tube

6. Base

•Bottom = support

7. Stage

•Where slide is placed

8. Stage Clips

•Hold slide in place

9. Mirror/ light

•Illuminates specimen, provides light

10.

Diaphragm

•Controls the AMOUNT of light used

11. CoarseAdjustmen

t Knob

•Larger focusing knob

•For focusing under LOW POWER ONLY

•If used under high power, objective lens and/or slide can break

12. Fine Adjustmen

tKnob

•Sharpens image•Focusing for High

Power

Mostpowerfulmicroscope

•Electron microscope: magnifies up to 1 million times

•Uses beam of electrons instead of light

Fly

Spider

Termite head

Tapeworm

Blood

SEM •Scanning Electron Microscope

• Produces real, 3D image• Only scans surface• magnifies 20x - to 200,000x

TEM •Transmission Electron Microscope

•Produces 2D image of thinly sliced organism

•magnifies 1,000x -1,000,000x

http://www.nims.go.jp/htm21/MA/tem.jpg

Phase ContrastMicroscope

•View living specimens without staining

Light microscope

Phase contrast microscope