Topic 7: Cellular Genetics.Exercise II Mitosis Cell Cycle

Exp: Cell cycle onion tips

Topic: DNAExercise I: Isolation Analysis Plasmid DNA

Onion root tip.

Onion root tip.

Onion root tip.

Structure of bacteria.

Two strains of bacteria: pBR322 and pUC18

Isolation of Plasmid DNA: 1. Collect bacteria.

Isolation of plasmid DNA:2. Add:

Isolation of Plasmid DNA:3. Precipitate proteins, chromosomal DNA, and some of the RNA.

Isolation of plasmid DNA:Note: Addition of very small quantities of liquids.

Isolation of plasmid DNA:4. DNAse and RNAse treatments.

Agarose Gel.

Agarose Gel.

Electrophoresis:

Electrophoresis:

Isolation of Plasmid DNA.

pBR322 pUC18

ET D R ET D R

pUC18 pBR322 ET D R ET R D