TMP-Bio for converting cellulosic biomass to 2nd ... · PDF fileTMP-Bio for Converting Cellulosic Biomass to 2nd Generation Sugar and Near-native Lignin Changbin Mao, Zhirun Yuan,

TMP-Bio for Converting Cellulosic Biomass to 2nd

Generation Sugar and Near-native Lignin

Changbin Mao, Zhirun Yuan, Luis Fernando Del Rio, Waleed Wafa Al Dajani, David Wong, Thomas Browne

FPInnovations, 570 Boul. Saint-Jean, Pointe-Claire, QC, H9R 3J9 Canada

International Forest Biorefining Conference (IFBC) May 9-11, 2017, Thunder Bay, Ontario, Canada

© 2015 FPInnovations. All rights reserved. Copying and redistribution prohibited. ® FPInnovations, its marks and logos are trademarks of FPInnovations.

Outline

2

Ø Introduction

Ø TMP-Bio Technology§ Unique Biomass Treatment Technology

§ Robust Enzymatic Hydrolysis Technology

Ø TMP-Bio Scaling-up

Ø Products from sugar and H-lignin

Ø Summary


Genome, Fibre attribute, Phytosanitary Bio-Products

New

Tec

hnol

ogie

s

Improved Forestry Inventory

FPInnovations / Upstream

Forest Harvesting SourcingIntegrated Forest Land-Use

Management


Creating Opportunities Beyond Traditional MarketsCr

eatin

g Va

lue

Adde

d èè

Develop New Processes and New Materials èè

Wood Products

Pulp & Paper

Chemicals CNC(NCC)

LigninCF Sugars Energy


Current Commercial Cellulosic Biorefineries

5* Ref. Novozymes 2014

§ Steam explosion (SE) is the predominant pretreatment technology for cellulosic biomass treatment

§ Develop a robust pretreatment technology is critical for cellulosic based biorefineries

M&G and Guozhen 2014 JVAnhui province, CNAgriculture residue ~ 80 MGY

© 2015 FPInnovations. All rights reserved. Copying and redistribution prohibited. ® FPInnovations, its marks and logos are trademarks of FPInnovations. 6

To Overcome The Challenges

Endoglucanase (EGI-V)

Non-reducing end Reducing end

Cellobiose

Exoglucanase CBH-IExoglucanase CBH-II

Glucose

Beta-glucosidase

1. Extensive intra-chain hydrogen bonding

2. Cellulose is embedded in HCs and Lignin matrix


TMP-Bio Process

7

Hydrolysis

1000 kg

500 kg sugars

400 kg

Biomass treatment

Digestible biomass

Bio products- Succinic acid- Lactic acid- Butanol- Ethanol - … …

New products- E-lignin- A-lignin - Activated carbon - Resin- Additives- … …

H-Lignin

Chemical treatment

I II


TMP-Bio is a CTMP/TMP-based Process

§ Further process development allows us to easily incorporate TMP-Bio technology in both CTMP and TMP mills


Pre-treatment and Post-treatment Pilot Trials

9

Hydrolysis conditions: 2% solids, 72 hours.

0

20

40

60

80

100

0 5 10 15 20

Gluc

an c

onve

rsio

n, %

Enzyme loading, FPU/g glucan

BR42A (Pilot post-treatment)BR17 (pilot pre-treatment)Post-treatmentPre-treatment


§ Low chemical, low energy, medium temperature

§ Biomass treatment can be conducted prior or post refiner

§ No need to extract HCs and lignin (Cel/HC/L, ~50/15/23)

§ High sugar conversion yield (90%+ at high solid)

§ Could use existing facilities at a large scale (+1000 ton/d)

Produce highly digestible biomass at large scale

Unique Biomass Treatment Technology


Robust Enzymatic Hydrolysis Technology

§ With the F3 at 20 FPU, E-Hydrolysis bottleneck disappeared with glucan and xylan being simultaneously hydrolyzed

0

20

40

60

80

100

120

0 24 48 72 96 120 144

Suga

r Con

vers

ion

Yiel

d, %

E- Hydrolysis Time, Hrs

Cellulase (CL) at 20 FPU

Xylose

Glucose

Formulation F3 at 20FPU

00 24 48 72 96 120 144

E-hydrolysis Time, Hrs

20

40

60

80

100

Suga

r Con

vers

ion

Yiel

d, %

Xylose

Glucose


Enzyme Formula Optimization (F3)

Estimated Response SurfaceBG=10.0

CtecHtec

gluc

an y

ield

0 10 20 30 40 0204060801000

20406080

100

0

20

40

60

80

100

0 20 40 60 80 100Pre

dic

ted

co

nve

rsio

n y

ield

, %

Observed conversion yield, %

fittedlower 95%upper 95%val-P %


Robust Additives

§ The additives work extremely well at low enzyme dosage (+50% at 5 FPU) § At 5 FPU, sugar conversion yield is +75% (6 days)

0

20

40

60

80

100

0 5 10 15 20 25

Glu

cose

yie

ld, %

Enzyme dosage, (Ctec1, FPU/g glucan)

0.038 0.076 0.114(ml enzyme/g cellulose)

+50%

F3 with add. (3d)

F3 with add. (6d)

Model without add. (3d)R2=98%


Towards High Solids Enzymatic Hydrolysis (2% vs. 20%)

§ Similar hydrolysis efficiency for both low and high solid loadings§ Much high sugar concentration at 20% solid loading

0

30

60

90

120

150

0 2 4 6Suga

r con

cent

ratio

n, g

/LHydrolysis time, days

Total sugar concentration

TSCon-2%

TSCon-20%

0

20

40

60

80

100

0 2 4 6

Ove

rall

Suga

r yie

ld, %

Hydrolysis time, days

Overall sugar yield

Sugar yield-2%

Sugar yield -20%


High solid (20%) Enzymatic Hydrolysis (50L)


High solid (20%) enzymatic hydrolysis (1,000L)

16

D2 10:178 barrels

Total sugar: 113.3g/L

D1 12:084 barrels

D1 14:416 barrels

D1 14:254 barrels

200L

200 OD kg Biomass at 30%


Sugar Lignin Separation

17


Refiner

Latency

Ligninfor drying

LC Reactor

Paddle Reactor

Centrifuge

Totes for shipping

Grinder

Screw press

Sugar

Tank

II

Digester

Biomass

Press Impregnator I

FPInnovations’ small TMP-Bio pilot plant (PC)

TMP-Bio Scale-up


Major Equipment of The Pilot Plant

19

Existing pilot equipment (A)

Installed new pilot equipment (B)

A1 A2 A3 A4

B1 B2 B3 B4


TMP-Bio pilot plant

20


Sugar Properties from Pilot Plant Operation


Sugar Quality from Pilot Plant Operation

22

Fresh sugar Concentrated sugar

Average pH 5,1 5,3

Average density (kg/L) 1,065 1,213

Glucose concentration (g/L) 102 320

Xylose concentration (g/L) 32 97

Furfural (g/L) Not detected 0,031

5-(Hydroxymethyl) furfural (g/L) Not detected 0,023

5-Methyl furfural (g/L) Not detected Not detected

2-Methyl furan Not detected Not detected

Acetic acid (g/L) 4,4 12,2

Hg, As, Pb, Cd (ppm) - <0.5

Fe, Mn, Cu (ppm) - <3.3

Ca (ppm) - 747

P (ppm) - 150

S (ppm) - 192


Lactic Acid Fermentation

§ Direct fermentation without detoxification§ Independent pilot trials confirmed sugar-free fermentation can

be achieved§ Lactic acid yield: 80-93% (w/w)

LA Lab result


H-lignin Powder Product

24

§ Light color, no-sulfur, properties close to native lignin

§ Particle size: 10-300 µm

Kraft ligninH-lignin


Towards Commercialization

25

Treatment Highly

digestible biomass(Cel/HC/L: 50/15/23)

Biomass

Enzymatic hydrolysisYd, +90%

Mixed sugar (con.>10%)Mixed sugar (con.>10%)

Hydrolysis lignin (contains ~60%

lignin)

• HE-lignin• HA-lignin • Resin• Additives• … … New products

• Succinic acid• Lactic acid• Butanol• Ethanol

Engineering Study on 1 ton/batch

Engineering Study on 1 ton/batch

100 ton/d500 ton/d


Summary

26

§ FPInnovations has developed a proprietary TMP-Bio technology for cellulosic biomass conversion (Patented in US, CA, NZ, AU, CN)

§ The unique biomass treatment technology can produce highly digestible biomass at industrial scale (1000ton/day)

§ Robust enzymatic hydrolysis technology can simultaneously convert both glucan and xylan at 90% at 20% solid loading

§ High quality sugar and near native H-lignin are produced in our pilot plant

§ Various valued-added products can be developed from TMP-Bio sugar and H-lignin


Acknowledgment

27

o The financial support from NRCAN, MRNF, partners and our member companies

o Novozymes for providing us Cellic series enzymes

o The ARS National Center for Agricultural Utilization Research, USDA, for providing us the strain for lactic acid

o Our technicians and other staff in FPInnovations, …...

o IFBC committee

www.fpinnovations.ca

Follow us on


Thank you Zhirun YuanResearch manager - Biorefinery [email protected]

Changbin MaoScientist - Biorefinery [email protected]

Documents

TMP-Bio for converting cellulosic biomass to 2nd ... · PDF fileTMP-Bio for Converting Cellulosic Biomass to 2nd Generation Sugar and Near-native Lignin Changbin Mao, Zhirun Yuan,