Upload
madlyn-oneal
View
216
Download
0
Tags:
Embed Size (px)
Citation preview
ISOLATION OF ANTIBIOTIC-PRODUCING
MICROORGANISMS
T.Monirah Alhammadi
There are several ways to isolate microbes
that produce antibiotics from the soil. Below are some methods used to isolate microbes produce antibiotics effective against some microbes
PRINCIPLE
Random Isolation in pure
cultur
crowded-plate
Catrer and lockwood method
Isolation of Antagonistic soil microorganisms
#soil isolation agar plate
pure culture test each culture against Plant Pathogenic
microbes inhibition zone # Demonstrates the effectiveness of
the microbe isolated from the soil against the microbe pathological
1-Random Isolation in pure cultur
1-Random Isolation in pure cultur
#fungus pathological pollination
growth media Incubate plates
#Then sprinkle dishes of soil dilution 1 \ 1000 to 1 \
100000 approximately 0.6 cm Incubate plates
Tset the plate inhibition zone
2 -Catrer and lockwood method
Catrer and lockwood method
]In this experiment , we will use the
crowded-plate technigue for isolation of antibiotic-producing microorganisms from soil
3-crowded-plate
#Soil suspensions(1:500 dilution of soil
sample suspension) #cultures of E.coli, S.aureus, (P.aeruginosa
#test tube #petri dishes
#1-ml and 5-ml pipettes #bunsen burner
#sterile water
Materials
1 -label three test tubes 1,2and3. with apipette , add 5ml of tap water to eash tube.
2 -shake the provided 1:500 soil sample thoroughly for 5 minutes to effect a uniform soil-water suspension.
3 -using a 5-ml pipette , transfer 5 ml from the 1:500 dilution to tube 1 and mix . The final dilution is 1:1000
4-using another pipette , transfer 5 ml from tube 1 to tube 2 and mix.
The final dilution is 1:20005-using another pipette , transfer 5 ml from tube 2
to tube 3 and mix. The final dilution is 1:4000
Methods:
.6-Using separate 1-ml pipettes , transfer 1 ml of the 1:1000, 1:2000
Dilutions to their appropriately labeled petri dilutions
agar ) trypticase soy ) 7- pour one tube of molten
8 -incubate all plates in an inverted position for 2 to 4 days at 25C
Methods:
THE END