Title:Genetic analysis of Soil-borne cereal mosaic virus response in durum wheat: Evidence for the role of the major QTL QSbm.ubo-2BS and of minor QTLs.

Journal: Molecular Breeding

Author’s names:Marco Maccaferri1, Rossella Francia1, Claudio Ratti1, Concepcion Rubies-Autonell1, Chiara Colalongo1, Gianluca Ferrazzano2, Roberto Tuberosa1, Maria Corinna Sanguineti1

Affiliations and addresses:1 Department of Agroenvironmental Science and Technology, University of Bologna, Viale G. Fanin 44, 40127 Bologna, Italy 2 Società Produttori Sementi Bologna S.p.A., Divisione Ricerca, Via Macero 1, 40050 Argelato (BO), Italy

corresponding author: marco.maccaferri@unibo.itPhone: +39 051 2096645Fax: +39 051 2096241

Supplementary material Figure 1. Frequency distributions of the phenotypic traits recorded for the Simeto × Levante (SxL) recombinant inbred line population (180 F6:7 RILs) in two field trials carried out in Cadriano (44°35’N 11°27’E), Northern Italy (Po valley), under high SBCMV-disease pressure during two seasons (2008 and 2009).

The following SBCMV-response traits were measured at the plant growth stages showing the maximum disease symptom intensity, i.e. from mid-end of tillering (Z25 in the Zadoks scale, Zadoks et al., 1974) to first (Z31) and second (Z32) node appearance stages (corresponding to a period from the end of February to April):

• symptom severity (SS), visually scored following a 0-4 scale at three and five scoring/sampling dates in 2008 and 2009, respectively;

• leaf virus concentration (ELISA), determined using DAS-ELISA (reported as absorbance units at 405 nm) on samples collected at two scoring/sampling dates in both 2008 and 2009.

Frequency distributions were obtained using the mean values across scoring/sampling dates of each year for SS, ELISA values, leaf greenness (SPAD) and Normalized Difference Vegetation Index (NDVI). Grain yield (GY) was also considered.Mean values for the RILs (S×L), Simeto (S), Levante (L) and for the highly susceptible check cv. Grazia (G) are indicated with arrows and reported together with the corresponding standard deviation in parenthesis.

Symptom severity (SS), score unit: scale 0-4

RIL

nu

mb

er

SxL RIL SS-2008

0 1 2 3 4

5

10

15

20

25

Virus concentration (ELISA values), A 405 nm

210.5 1.50

10

20

30

SxL RIL ELISA-2008

G (

3.42

± 0

.29)

L (

0.13

± 0

.10)

S (

2.78

± 0

.17)

G (

1.41

± 0

.16)

L (

0.02

± 0

.01)

S (

1.78

± 0

.12)

Leaf greenness (SPAD), SPAD units 4540 5035

10

20

30

40

50

643 52 7 8 9

10

20

30

0.500.45 0.550.40

10

20

30

40

50

Spectral reflectance (NDVI), NDVI units 0.60 0.65 0.70

SxL RIL SPAD-2008 SxL RIL SR-2008

SxL RIL GY-2008

Grain yield (GY), t ha-1

G (

43.0

± 2

.58)

L (

52.2

± 0

.10)

S (

43.7

± 1

.62)

G (

0.58

± 0

.07)

L (

0.61

± 0

.10)

S (

0.54

± 0

.06)

S (

5.41

± 0

.18)

L (

7.74

± 0

.11)

G (

2.17

± 0

.50)

55

RIL

nu

mb

er

SxL RIL SS-2009

0 1 2 3 4

5

10

15

20

25

210.5 1.50

10

20

30

SxL RIL ELISA-2009

G (

3.81

± 0

.25)

L (

0.65

± 0

.35)

S (

2.88

± 0

.17)

G (

1.62

± 0

.08)

L (

0.17

± 0

.06)

S (

1.50

± 0

.15)

Symptom severity (SS), score unit: scale 0-4 Virus concentration (ELISA values), A 405 nm

Leaf greenness (SPAD), SPAD units 3530 4025

10

20

30

40

50

643 52 7 8 9

10

20

30

0.350.30 0.40

10

20

30

Spectral reflectance (NDVI), NDVI units 0.45 0.50 0.55

SxL RIL SPAD-2009 SxL RIL NDVI-2009

SxL RIL GY-2009

Grain yield (GY), t ha-1

45 50 0.60

G (

30.2

± 2

.60)

L (

43.4

± 2

.00)

S (

32.6

± 2

.21)

G (

0.38

± 0

.06)

L (

0.59

± 0

.04)

S (

0.47

± 0

.03)

G (

3.67

± 0

.33)

L (

7.40

± 1

.46)

S (

5.00

± 0

.75)

Supplementary material Figure 2.

Location of the QTLs controlling the SBCMV response in the Simeto × Levante recombinant inbred line (RIL) population, as evaluated in two field trials carried out in 2008 and in 2009. The location of the chromosome regions that were found significant on the basis of the composite interval mapping (CIM) is reported on the right side of the chromosome bars for each trait and for each scoring/sampling date.

QTLs for the following traits are reported: visually estimated symptom severity (SS), virus concentration in the leaves (ELISA) and grain yield (GY). QTLs for GY were reported only when their positions overlapped with those of QTLs for direct SBCMV-response traits. The QTLs are indicated with acronyms including the trait acronym and the scoring/sampling date (indicated with a progressive number from 1 to 5, see materials and methods section for the corresponding dates).

QTLs are indicated with vertical lines spanning the significance intervals (LOD = 2) and with triangles pointing to the QTL peak position. At each QTL region, the parent contributing the favorable allele has been reported within curly brackets (S = Simeto or L = Levante).

gwm33 0.0

wPt-0595 15.2wPt-6280 17.7wPt-1924 17.8

wPt-3870-a 26.3wPt-4177 27.5

wPt-6882 wPt-4735 36.6tPt-5413 39.8

barc158 0.0barc211 2.0

gwm1139 3.0

SxL1A1

wPt-8971 wPt-4726 0.0wPt-3889 0.9

wmc500.1 27.6barc240 28.9

wPt-1374 29.7

wPt-2389 46.7gwm131 48.0

wPt-3451 49.2

wmc664 62.3

barc61 79.9wPt-6690 wPt-8280 81.2

cfd48 82.6

rPt-7906 98.2wPt-1247 99.2wPt-9409 99.4wPt-1818 99.5

wPt-6142 wPt-3950 0.0wPt-5915 wPt-2968 0.3

wmc728 0.9

SxL1B1

wmc667 0.0wmc407 4.0barc212 5.0tPt-2481 12.0

wPt-8925 12.2wPt-0102 wPt-0277wPt-3611 wPt-3744 12.3

tPt-1041 wPt-4533wPt-4197 wPt-5647 17.5

wPt-7049 17.8wPt-6245 18.2tPt-5584 30.3

wPt-9624 wPt-5839wPt-7626 32.3

cfa2201 64.7gwm1198 68.5wPt-7026 71.4wmc453 76.3wmc522 80.6

wmc296 91.3gwm122 91.6

gwm425 gwm339 95.8gwm95 96.4

gwm558 102.9wPt-8826 104.5

cfa2263 104.9gwm372 109.1

barc91 0.0cfa2058 1.0gwm312 6.4gwm47 8.8

gwm294 8.9wPt-2293 wPt-3244 16.0

barc183-a 18.3

tPt-6105 0.0cfd50 2.4

wPt-6064 4.3wPt-8403 4.9wPt-9797 6.2

wPt-9793 wPt-9277 18.4gdm93 20.2

wPt-1108 27.8wPt-1320 31.6

SxL2A1gwm33 0.0

wPt-0595 15.2wPt-6280 17.7wPt-1924 17.8

wPt-3870-a 26.3wPt-4177 27.5

wPt-6882 wPt-4735 36.6tPt-5413 39.8

barc158 0.0barc211 2.0

gwm1139 3.0

SxL1A1

wPt-8971 wPt-4726 0.0wPt-3889 0.9

wmc500.1 27.6barc240 28.9

wPt-1374 29.7

wPt-2389 46.7gwm131 48.0

wPt-3451 49.2

wmc664 62.3

barc61 79.9wPt-6690 wPt-8280 81.2

cfd48 82.6

rPt-7906 98.2wPt-1247 99.2wPt-9409 99.4wPt-1818 99.5

wPt-6142 wPt-3950 0.0wPt-5915 wPt-2968 0.3

wmc728 0.9

SxL1B1

wmc667 0.0wmc407 4.0barc212 5.0tPt-2481 12.0

wPt-8925 12.2wPt-0102 wPt-0277wPt-3611 wPt-3744 12.3

tPt-1041 wPt-4533wPt-4197 wPt-5647 17.5

wPt-7049 17.8wPt-6245 18.2tPt-5584 30.3

wPt-9624 wPt-5839wPt-7626 32.3

cfa2201 64.7gwm1198 68.5wPt-7026 71.4wmc453 76.3wmc522 80.6

wmc296 91.3gwm122 91.6

gwm425 gwm339 95.8gwm95 96.4

gwm558 102.9wPt-8826 104.5

cfa2263 104.9gwm372 109.1

barc91 0.0cfa2058 1.0gwm312 6.4

gwm47 8.8gwm294 8.9

wPt-2293 wPt-3244 16.0barc183-a 18.3

tPt-6105 0.0cfd50 2.4

wPt-6064 4.3wPt-8403 4.9wPt-9797 6.2

wPt-9793 wPt-9277 18.4gdm93 20.2

wPt-1108 27.8wPt-1320 31.6

SxL2A1gwm33 0.0

wPt-0595 15.2wPt-6280 17.7wPt-1924 17.8

wPt-3870-a 26.3wPt-4177 27.5

wPt-6882 wPt-4735 36.6tPt-5413 39.8

barc158 0.0barc211 2.0

gwm1139 3.0

SxL1A1

wPt-8971 wPt-4726 0.0wPt-3889 0.9

wmc500.1 27.6barc240 28.9

wPt-1374 29.7

wPt-2389 46.7gwm131 48.0

wPt-3451 49.2

wmc664 62.3

barc61 79.9wPt-6690 wPt-8280 81.2

cfd48 82.6

rPt-7906 98.2wPt-1247 99.2wPt-9409 99.4wPt-1818 99.5

wPt-6142 wPt-3950 0.0wPt-5915 wPt-2968 0.3

wmc728 0.9

SxL1B1

wmc667 0.0wmc407 4.0barc212 5.0tPt-2481 12.0

wPt-8925 12.2wPt-0102 wPt-0277wPt-3611 wPt-3744 12.3

tPt-1041 wPt-4533wPt-4197 wPt-5647 17.5

wPt-7049 17.8wPt-6245 18.2tPt-5584 30.3

wPt-9624 wPt-5839wPt-7626 32.3

cfa2201 64.7gwm1198 68.5wPt-7026 71.4wmc453 76.3wmc522 80.6

wmc296 91.3gwm122 91.6

gwm425 gwm339 95.8gwm95 96.4

gwm558 102.9wPt-8826 104.5

cfa2263 104.9gwm372 109.1

barc91 0.0cfa2058 1.0gwm312 6.4

gwm47 8.8gwm294 8.9

wPt-2293 wPt-3244 16.0barc183-a 18.3

tPt-6105 0.0cfd50 2.4

wPt-6064 4.3wPt-8403 4.9wPt-9797 6.2

wPt-9793 wPt-9277 18.4gdm93 20.2

wPt-1108 27.8wPt-1320 31.6

SxL2A1

SS

-1-0

8

SS

-2-0

8

SS

-3-0

8

SS

-2-0

9

SS

-1-0

9

SS

-3-0

9

SS

-4-0

9

SS

-5-0

9

EL

ISA

-4-0

9

EL

ISA

-2-0

9

GY

-09

L

Chr. 2A

wmc382 0.0wPt-3517 5.7wPt-8737 6.1wPt-0100 6.5wPt-2106 21.3wPt-9958 24.5wmc154 39.1wmc213 40.6wmc243 41.8tPt-4627 43.6

mag3976 43.7wmc25 wPt-8404 44.7

wPt-7158-b 55.0barc318 57.0

wPt-3561 57.8gwm429 66.6

wPt-6199 66.9wPt-7320-b wPt-2600-b 73.2wPt-7757-b wPt-6192-bwPt-4125-b wPt-5672-b

wPt-5556-b74.7

wmc770 75.6barc183-b 79.7

wPt-0724 wPt-9316 80.1wPt-2314-b 81.7

wmc27 100.2wmc474 103.8barc230 108.7

wPt-2664 111.7gwm55.1 112.1wmc265 113.0

gwm55.2 118.7

wmc332 0.0wPt-3755 2.3wPt-0510 9.4wPt-7004 9.9wPt-5989 12.3wPt-4223 13.4gwm1070 16.6

wmc361 0.0wPt-4426 0.2wPt-3632 0.4

wPt-2929 wPt-2502wPt-4892 1.2

SxL2B1

wPt-4480 0.0wPt-9373 0.1

wPt-3476 wPt-0096wPt-8585 wPt-0546 0.2

wPt-1331 0.3barc57 2.8

wPt-6204 4.1barc294 4.7

wPt-7992 wPt-9369wPt-6854 7.9

barc12 8.6wPt-2938 13.1

wPt-1655 wPt-1111 13.2

barc45 0.0gwm5 2.1

cfa2234 11.6

wPt-5886 33.1wmc428 34.8

wmc264 47.9cfa2262 51.1

wPt-11619 56.1wPt-8362 wPt-1119 57.1

tPt-7209 wPt-6956wPt-5943 67.6

wPt-3133 68.4wPt-3816 wPt-2659

wPt-1562 75.2cfa2076 0.0wPt-5133 wPt-5125

tPt-7492 6.9

gwm1038 9.2

tPt-6376 19.9

barc1113 29.1wPt-8203 29.9

barc1177 wPt-9160wPt-0398 37.0

SxL3A1

barc75 wPt-6973tPt-1366 0.0

wPt-6604 rPt-3056wPt-6235 0.4

wPt-1159 5.0wPt-6945 8.3wPt-5390 16.1

wPt-5939 wPt-9432wPt-9510 17.5

wmc418 gwm685 19.2wmc625 24.1

wPt-11691 barc84 0.0

wPt-3085 17.3wPt-1020 wPt-4412 18.4

wPt-2416 24.6tPt-4541 24.9wmc274 25.0

wPt-6961 wPt-8764wPt-0280 25.1

gwm181 26.1wmc632 27.0

SxL3B1

SS

-1-0

8

SS

-2-0

8

SS

-3-0

8

SS

-2-0

9

SS

-1-0

9

EL

ISA

-3-0

8

EL

ISA

-2-0

8

GY

-08

SS

-3-0

9

SS

-4-0

9

SS

-5-0

9

EL

ISA

-2-0

9

EL

ISA

-4-0

9

GY

-09

L

Chr. 2B

wPt-4480 0.0wPt-9373 0.1

wPt-3476 wPt-0096 wPt-8585wPt-0546 0.2

wPt-1331 0.3barc57 2.8

wPt-6204 4.1barc294 4.7

wPt-7992 wPt-9369 wPt-6854 7.9barc12 8.6

wPt-2938 13.1wPt-1655 wPt-1111 13.2

barc45 0.0gwm5 2.1

cfa2234 11.6

wPt-5886 33.1wmc428 34.8

wmc264 47.9cfa2262 51.1

wPt-11619 56.1wPt-8362 wPt-1119 57.1

tPt-7209 wPt-6956 wPt-5943 67.6wPt-3133 68.4

wPt-3816 wPt-2659 wPt-1562 75.2

cfa2076 0.0

wPt-5133 wPt-5125 tPt-7492 6.9gwm1038 9.2

tPt-6376 19.9

barc1113 29.1wPt-8203 29.9

barc1177 wPt-9160 wPt-0398 37.0

SxL3A1

wPt-6668 0.0wPt-2056 1.2

wPt-7767 wPt-1076 wPt-3836wPt-4172 wPt-6273 1.5

wPt-5092 3.7wPt-0002 5.2wPt-7830 5.7wPt-9496 7.5

wPt-5257 wPt-4993 wPt-4625tPt-6794 wPt-6184 8.0

wPt-5101 8.3wPt-1441 10.4wPt-0393 10.8wPt-3135 11.1

barc70 14.6wPt-6824 15.0wmc479 16.1barc246 44.1

wPt-7188 50.7wPt-3883 wPt-7734 wPt-9796 57.8

wPt-7214 58.2wPt-1163 63.7

wPt-11672 64.1barc154 66.1

wPt-7785 70.2cfa2028 70.6barc222 89.8

wmc405 97.8

wmc488 0.0wPt-6842 4.6wPt-8399 5.0

gwm276 13.8wPt-4877 14.9

wPt-5949 23.5

wPt-7053 33.5

wPt-0961 43.5wPt-4831 43.9

wPt-0745 wPt-7763 0.0wPt-1429 2.2

gwm1061 wPt-8365 2.5cfa2257 3.9

gwm344.2 4.9

SxL7A1

SS

-1-0

8

SS

-2-0

8

SS

-3-0

8

SS

-2-0

9

GY

-08 SS

-3-0

9

EL

ISA

-4-0

9

SS

-1-0

9

SS

-4-0

9

SS

-5-0

9

EL

ISA

-2-0

9 S

Chr. 3A

gwm610 0.0wPt-9037 4.1gwm894 0.0

wPt-8443 2.9wPt-2221 3.3wPt-7355 6.9

barc155 8.6barc236 11.1wmc161 19.2

wPt-2247 34.1wPt-3401 35.9wPt-1550 36.0wPt-1262 36.3wPt-2946 36.4

wPt-7558 wPt-4240 36.5wPt-1584 37.5wPt-7939 41.9wPt-6728 42.9rPt-7285 43.5

wPt-3810 44.1wPt-7926 47.6

cfd31 48.7wmc262 49.9

wPt-3655 50.5barc135 52.4

wPt-0105 wPt-3766wPt-9833 wPt-5749

wPt-621458.1

wPt-4596 63.4wPt-7821 wPt-6502wPt-3506 wPt-4241 74.4

wPt-3796 wPt-2985wPt-8886 wPt-8271wPt-3108 wPt-8167

wPt-2331

78.1

wPt-9196 78.3wPt-3449 78.5

wPt-1155 wPt-4424wPt-9418 88.3

wmc219 88.7wPt-0764 wPt-8657wPt-7876 wPt-9059 89.7

wPt-8171 92.7wmc313 92.9

wPt-2151 95.2wPt-11661 98.3

rPt-0238 wPt-5172 98.4barc52 111.3

SxL4A1

tPt-0517-a 0.0wmc9 5.6

wmc546-1 8.8

gwm368 0.0wmc89 2.1

wmc695 4.6wPt-0872 wPt-4931 8.3tPt-0602 wPt-3908 8.6

gwm495 24.4

gwm192 29.5

wPt-2214 64.7rPt-6847 73.8

cfd39 74.3wPt-8291 78.7

wPt-3255 wPt-11493wPt-9223 80.2

barc60 80.9wPt-8543 81.2

gwm6 84.5wmc47 86.4

cfd54 93.3

SxL4B1

gwm154 0.0barc316 gwm1574 3.2

wmc489 9.9barc186 16.4barc117 19.1gwm129 20.5barc180 21.9

wPt-5751 29.2

gwm156 35.8wPt-2543 wPt-6825

wPt-4248 39.1

barc197 57.9

wPt-7201 85.6tPt-0353 85.9

barc151 97.0

gwm1570 104.8

barc319 120.4cfa2141 121.5

wPt-7111 0.0wPt-7804 0.3

wPt-4043 18.7gwm126 22.4

SxL5A1gwm610 0.0

wPt-9037 4.1gwm894 0.0

wPt-8443 2.9wPt-2221 3.3wPt-7355 6.9

barc155 8.6barc236 11.1wmc161 19.2

wPt-2247 34.1wPt-3401 35.9wPt-1550 36.0wPt-1262 36.3wPt-2946 36.4

wPt-7558 wPt-4240 36.5wPt-1584 37.5wPt-7939 41.9wPt-6728 42.9rPt-7285 43.5

wPt-3810 44.1wPt-7926 47.6

cfd31 48.7wmc262 49.9

wPt-3655 50.5barc135 52.4

wPt-0105 wPt-3766wPt-9833 wPt-5749

wPt-621458.1

wPt-4596 63.4wPt-7821 wPt-6502wPt-3506 wPt-4241 74.4

wPt-3796 wPt-2985wPt-8886 wPt-8271wPt-3108 wPt-8167

wPt-2331

78.1

wPt-9196 78.3wPt-3449 78.5

wPt-1155 wPt-4424wPt-9418 88.3

wmc219 88.7wPt-0764 wPt-8657wPt-7876 wPt-9059 89.7

wPt-8171 92.7wmc313 92.9

wPt-2151 95.2wPt-11661 98.3

rPt-0238 wPt-5172 98.4barc52 111.3

SxL4A1

tPt-0517-a 0.0wmc9 5.6

wmc546-1 8.8

gwm368 0.0wmc89 2.1

wmc695 4.6wPt-0872 wPt-4931 8.3tPt-0602 wPt-3908 8.6

gwm495 24.4

gwm192 29.5

wPt-2214 64.7rPt-6847 73.8

cfd39 74.3wPt-8291 78.7

wPt-3255 wPt-11493wPt-9223 80.2

barc60 80.9wPt-8543 81.2

gwm6 84.5wmc47 86.4

cfd54 93.3

SxL4B1

gwm154 0.0barc316 gwm1574 3.2

wmc489 9.9barc186 16.4barc117 19.1gwm129 20.5barc180 21.9

wPt-5751 29.2

gwm156 35.8wPt-2543 wPt-6825

wPt-4248 39.1

barc197 57.9

wPt-7201 85.6tPt-0353 85.9

barc151 97.0

gwm1570 104.8

barc319 120.4cfa2141 121.5

wPt-7111 0.0wPt-7804 0.3

wPt-4043 18.7gwm126 22.4

SxL5A1

SS

-2-0

8

EL

ISA

-4-0

8

GY

-08

EL

ISA

-4-0

9

GY

-08

SS

-1-0

9

L

L

Chr. 4BChr. 5A

wmc419 0.0gwm132 1.2wmc486 11.5

wPt-0073 wPt-3424 wPt-1725 14.9wPt-5673 18.2

wPt-7343 wPt-2714 19.2wPt-1922 20.6

wPt-8563 wPt-9015 wPt-9990 wPt-3130wPt-5383 wPt-11648 wPt-4720 wPt-6563

wPt-7150 wPt-438620.7

wPt-4031 21.7wPt-6293 26.5wPt-0151 27.6wPt-0882 27.9

wPt-3800 wPt-4678 wPt-8239 28.0wPt-7582 wPt-3116 wPt-1852 wPt-3304wPt-0245 wPt-8336 wPt-2689 wPt-9532

wPt-1547 wPt-7777 wPt-699429.3

wPt-2133 wPt-0351 31.1wPt-8814 wPt-5256 46.1wPt-6017 wPt-8153 48.5

wPt-3309-b 57.7wPt-5333-b 58.0wPt-4742-b 58.4

wPt-7540 61.8wPt-0554 62.2wPt-8783 67.1

barc68 wPt-6247 71.0gwm518 72.3wmc398 87.5

barc198 wmc744 101.3gwm193 102.4

barc354 120.0

wPt-1730 124.7

wPt-8508 wPt-8554 133.0

barc24 139.2

SxL6B

SS

-3-0

9

SS

-4-0

9

wPt-4480 0.0wPt-9373 0.1

wPt-3476 wPt-0096 wPt-8585wPt-0546 0.2

wPt-1331 0.3barc57 2.8

wPt-6204 4.1barc294 4.7

wPt-7992 wPt-9369 wPt-6854 7.9barc12 8.6

wPt-2938 13.1wPt-1655 wPt-1111 13.2

barc45 0.0gwm5 2.1

cfa2234 11.6

wPt-5886 33.1wmc428 34.8

wmc264 47.9cfa2262 51.1

wPt-11619 56.1wPt-8362 wPt-1119 57.1

tPt-7209 wPt-6956 wPt-5943 67.6wPt-3133 68.4

wPt-3816 wPt-2659 wPt-1562 75.2

cfa2076 0.0

wPt-5133 wPt-5125 tPt-7492 6.9gwm1038 9.2

tPt-6376 19.9

barc1113 29.1wPt-8203 29.9

barc1177 wPt-9160 wPt-0398 37.0

SxL3A1

wPt-6668 0.0wPt-2056 1.2

wPt-7767 wPt-1076 wPt-3836wPt-4172 wPt-6273 1.5

wPt-5092 3.7wPt-0002 5.2wPt-7830 5.7wPt-9496 7.5

wPt-5257 wPt-4993 wPt-4625tPt-6794 wPt-6184 8.0

wPt-5101 8.3wPt-1441 10.4wPt-0393 10.8wPt-3135 11.1

barc70 14.6wPt-6824 15.0wmc479 16.1barc246 44.1

wPt-7188 50.7wPt-3883 wPt-7734 wPt-9796 57.8

wPt-7214 58.2wPt-1163 63.7

wPt-11672 64.1barc154 66.1

wPt-7785 70.2cfa2028 70.6barc222 89.8

wmc405 97.8

wmc488 0.0wPt-6842 4.6wPt-8399 5.0

gwm276 13.8wPt-4877 14.9

wPt-5949 23.5

wPt-7053 33.5

wPt-0961 43.5wPt-4831 43.9

wPt-0745 wPt-7763 0.0wPt-1429 2.2

gwm1061 wPt-8365 2.5cfa2257 3.9

gwm344.2 4.9

SxL7A1

SS

-3-0

8

GY

-08

L

S

Chr. 6B Chr. 7A

wPt-8211 wPt-4843 wPt-0837 0.0wPt-5846-a wPt-3147-a wPt-7975-a 3.7

wPt-5283 9.9

gwm400 0.0

wPt-7064 12.1wPt-1723 wPt-8390 wPt-8283 12.4

gwm573 28.7wmc182 29.0

gwm1184 36.0gwm3019 39.9

wPt-2737 44.6

gwm333 59.3wmc396 84.4

wPt-11598 95.3wPt-2305 97.2wPt-3730 99.8

wPt-4025 wPt-8919 104.1wPt-1817 wPt-4323 wPt-0980 112.0

wPt-9665 119.0wPt-2308 127.2wmc517 127.8barc315 132.6

wPt-5922 138.5wPt-5975 143.5wPt-7887 143.8wPt-5892 143.9

wPt-8615 wPt-5343 149.1wPt-2668 157.8

wPt-11688 158.1wPt-1715 wPt-4298 tPt-7362

wPt-7351 wPt-4010 wPt-4869 158.2

wPt-8417 161.6wPt-4814 164.5wPt-4045 169.0

wPt-11630 170.3gwm611 173.8

wPt-3086 wPt-4300 177.7wPt-6156 179.3gwm783 180.1wmc276 187.0cfa2040 188.6

wPt-1957 wPt-5462 190.2barc182 192.1

barc32 193.3wPt-5138 193.6

wPt-8387 wPt-7108 195.6wPt-2449 wPt-0884 203.2

ubw14 204.5mag3433 208.4

wPt-5677 wPt-0126 wPt-0494wPt-3785 wPt-6320 wPt-0465 210.0

wPt-6276 210.9wPt-0477 213.9

wPt-7219 wPt-6869 tPt-3700 215.6wPt-3439 215.8

wPt-5585 wPt-4743 wPt-4038 216.0wPt-7387 216.4gwm146 219.3

gwm344.1 219.6YP-7B1 227.1

SxL7B1

EL

ISA

-2-0

8

GY

-08

L

Chr. 7B