TISSUE CULTURE WITH BIOREACTORTISSUE CULTURE WITH BIOREACTOR

TISSUE CULTURE WITH BIOREACTOR

Tissue culture is the growth of tissues and/or cells separate from the organism. This is typically facilitated via use of a liquid, semi-solid, or solid growth medium, such as broth or agar.

Temporary Immersion System (TIS)

T I i S (TIS) i fTemporary Immersion System (TIS) is a type of bioreactor where plant tissues are immersed in the vessel that contains the artificial liquid medium The whole system iscontains the artificial liquid medium. The whole system is maintained under sterile conditions. This is mostly used for plantlet production of various plant species. There is an p p p pautomatic system by which the growing tissues are immersed in the medium for some time and then they are lifted up. By this

h d h h i h f h dimethod they get the nourishment from the medium.This system is convenient device for the control of the

airflow and the length of liquid immersion The design is toairflow and the length of liquid immersion. The design is to achieve uniform experimental conditions easily. The device can accurately control the air inflow and immersion frequency.

Temporary Immersion System (TIS)

TIS ADVANTAGESTIS ADVANTAGES

1. Periodic medium immersion2. Higher plant multiply3. Space reductionp4. Manpower reduction5. Cost reduction (Liquid medium)( q )6. Decontamination with filter (0.2 µm)7. Gas exchangeableg

TIG - Temporary Immersion Gravity Flask Bioreactor

Ref Request No: 1001001111

The main feature of TIG (Temporary Immersion Gravity Flask Bioreactor) is based on flask which the inner space is connected by o eacto ) s based o as w c t e e space s co ected bystainless tube. When the low pressure air injecting through the inlet, the medium will be pumped up to the culture above. For a meanwhile, the medium goes down to the bottom when the air pressure is released by gravity

TIG ADVANTAGES

Fit f h dlFit for handle

Easy to use, No complicate

Decontamination with close technique

Ph l i i TIG Phalaenopsis in TIG (Temporary Immersion Gravity Flask Bioreactor)

We set experiment for 2 varieties in Phalaenopsis

TIGS lid M diSolid Medium

X Y

- 1 monthCh i di

- 1 monthCh i di

Variety A

- Changing medium 1 time/2 weeks- 6 mins/1 time

6 times/day

- Changing medium 1 time/2 weeks- 6 mins/1 time

4 times/day2 months

- 6 times/day - 4 times/day

- 1 month - 1 month

Variety B

- Changing medium 1 time/2 weeks- 6mins/1 time

6 times/da

- Changing medium 1 time/2 weeks- 6 mins/1 time

4 times/da2 months

- 6 times/day - 4 times/day

Phalaenopsis in TIG (Temporary Immersion Gravity Flask Bioreactor)

TIG

Conclusion: Multiply Rate in Phalaenopsis

TIGSolid Medium

X Y

Variety A 1.4 1.0 0.7Variety A 1.4 1.0 0.7Variety B 1.2 1.0 0.7

TIG Ti C lt i Ph l iTIG Tissue Culture in PhalaenopsisDetail TIG Solid Medium

Volume of container (ml) 700 500

Volume of medium (ml) 100 100( )

Space 1 x 1.5 m 80 96

Time (week) 4 8( )

Cost of medium/liter 70 100

Quantities of initiation 25 25Q

Quantities of multiply 125 75

Factor of TIG efficiency system1. Quantities of Initiation and Volume of Medium2. Number and Time of medium immersion

Ginger in TIG (Temporary Immersion Gravity Flask Bioreactor)

Multiply plant in Transplant 80 – 90 % p y pTIG 6.56 times

p

Banana in TIG (Temporary Immersion Gravity Flask Bioreactor)

M lti l l t iMultiply plant in

TIG 4.05 times

Transplant 80 90 %Transplant 80 – 90 %

TIG Reference

A i f l bAccessories for laboratory

Thank you