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Figure 1. 50 ng/ml NGF. 5 ng/ml NGF. A. NGF + SB203580. Control. NGF + SB202190. NGF. B. 8. 6. p38 MAPK phosphorylation (Fold stimulation). 4. 2. 0. 10. 20. 30. Time (min). P-p38 T-p38. 5 ng/ml NGF. P-p38 T-p38. 50 ng/ml NGF. Time (min). 0. 1. 3. 5. 10. 15. - PowerPoint PPT Presentation
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Time (min)
Time (min) 0 1 3 5 10 15 20 30
50 ng/ml NGF
P-p38T-p38
5 ng/mlNGF
300
100
503010510
NGF (ng/ml)
0 10 20 30
50 ng/ml NGF 5 ng/ml NGF
8
Log [NGF] (ng/ml)
p38
MA
PK
ph
osp
hor
ylat
ion
(Fol
d s
tim
ula
tion
)
p38
MA
PK
p
hos
ph
oryl
atio
n(F
old
sti
mu
lati
on)
0
2
4
6
8
100 10001 10
2
6
4
P-p38T-p38
P-p38T-p38
B
C
NGF
ControlNGF +
SB203580
NGF +SB202190
AFigure 1
C
0
2
4
6Basal 5 ng/ml NGF
PTX
PTX
2
4
6
0
#
**
p38
MA
PK
ph
osp
hor
ylat
ion
(
Fol
d s
tim
ula
tion
)
# #
*
** **
* *
Con
trol
G z
G oA
CI
G i2
/z
G oA
G i2
P-p38T-p38
P-p38T-p38
p38
MA
PK
ph
osp
hor
ylat
ion
(Fol
d s
tim
ula
tion
)
6
4
2
0
PTX
Control 5 ng/ml NGF
50 ng/ml NGF
#
8
* *#
A
P-p38T-p38
Figure 2
PTX
NGF
P-p38T-p38
B
Gi2GTGi2
PTX
2
4
6
0P-p38T-p38
#
2
4
6
0
*
P-p38T-p38
PTXB
p38
MA
PK
ph
osp
hor
ylat
ion
(
Fol
d s
tim
ula
tion
)
A
IgG
Trk
A
IgG
Trk
A
NGF NGF
P-TrkA
Gi2
Gz
Go
WB
IP IP
Lys
ate
Lys
ate
Figure 3
A
0
2
4
6
*
p38
MA
PK
ph
osp
hor
ylat
ion
(F
old
sti
mu
lati
on)
*
#
*
U01
26
Con
trol
NG
F
P-p38T-p38
U01
24
SP
6001
25
BBasal 5 ng/ml NGF 5 ng/ml NGF + inhibitor
MA
PK
ph
osp
hor
ylat
ion
(F
old
sti
mu
lati
on)
*
#048
10*
#
1214
*
P-MAPK
T-MAPK
U01
24U
0126
Con
trol
NG
F
SP
6001
25
Con
trol
NG
F
JNKERK
Figure 4
0
2
4
6
Con
trol
NG
FA
G49
0
SU
6656
PP
1
p38
MA
PK
p
hos
ph
oryl
atio
n
(Fol
d s
tim
ula
tion
)
PP
2P
P3
**
# #
#
**
AG
1295
ABasal 5 ng/ml NGF 5 ng/ml NGF + inhibitor
B
P-p38T-p38
W7
Con
trol
NG
FK
N62
Sta
uro
spor
ine
U73
122
Cal
ph
osti
n C
U73
343
* **
**
* *
Src
ph
osp
hor
ylat
ion
(Fol
d s
tim
ula
tion
)
0
PTX
P-Src
T-Src
1
2 *3
#
C
Figure 5
C3GC3G
Rap1Rap1
RhoARhoA
RhoGEF
RhoGEF
GG
B-RafB-Raf
NGF
Trk A receptor
CrkCrk
GIPC1GIPC1GAIP
GAIP
p38 MAPK
Rac/cdc42
Rac/cdc42
MKK3/6MKK3/6
MLKsMLKs
MKK4MKK4
MEKK1-4MEKK1-4
RasRas
Gi2
Gi2
MEK1/2
Gi2 Src
Src
Figure 6
